keyword
MENU ▼
Read by QxMD icon Read
search

Labeling

keyword
https://www.readbyqxmd.com/read/29471557/prospective-comparison-of-a-metal-free-ceramic-total-knee-arthroplasty-with-an-identical-metal-system
#1
Klemens Trieb, David Ullmann, Katharina Metzinger, Reinhard Kramar, Andreas Mauerer, Stefan Hofstätter, Rainer Fiala
AIMS: The aim of this open-label, prospective, short-term study was to carry out an initial comparison of a completely metal-free ceramic with a geometrically identical metallic arthroplasty over a 1-year follow-up period. METHODS: This study investigates a completely metal-free system using a composite matrix material containing aluminum oxide (Al2 O3 , BPK-S Integration, Peter Brehm GmbH, Weisendorf, Germany) or zirconium oxide (ZrO2 , BPK-S Integration Ceramic, Biolox Delta-CeramTec GmbH, Plochingen, Germany)...
February 2018: Zeitschrift Für Orthopädie und Unfallchirurgie
https://www.readbyqxmd.com/read/29471282/aunps-cnos-swcnts-chitosan-nanocomposite-modified-electrochemical-sensor-for-the-label-free-detection-of-carcinoembryonic-antigen
#2
Mohammad Rizwan, Syazwani Elma, Syazana Abdullah Lim, Minhaz Uddin Ahmed
In this work, a nanocomposite of gold nanoparticles (AuNPs), carbon nano-onions (CNOs), single-walled carbon nanotubes (SWCNTs) and chitosan (CS) (AuNPs/CNOs/SWCNTs/CS) was prepared for the development of highly sensitive electrochemical immunosensor for the detection of carcinoembryonic antigen (CEA), clinical tumor marker. Firstly, layer-by-layer fabrication of the CEA-immunosensors was studied using cyclic voltammetry (CV) and square wave voltammetry (SWV). By combining the advantages of large surface area and electronic properties of AuNPs, CNOs, SWCNTs, and film forming properties of CS, AuNPs/CNOs/SWCNTs/CS-nanocomposite-modified glassy carbon electrode showed a 200% increase in effective surface area and electronic conductivity...
February 14, 2018: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/29471224/quantification-of-desoxyrhapontigenin-4-methoxyresveratrol-in-rat-plasma-by-lc-ms-ms-application-to-pre-clinical-pharmacokinetic-study
#3
Yu Dai, Amanda Li Cheng Tan, Huan Chen, Pei Shi Ong, Xiaoqiang Xiang, Jinzhu Wu, Hai-Shu Lin
Desoxyrhapontigenin (DRG, 4-methoxyresveratrol or trans-3,5-dihydroxy-4'-methoxystilbene) is a naturally occurring resveratrol (RES) derivative with a variety of biological activities. To facilitate its further medicinal exploration, a reliable LC-MS/MS method was developed and validated for the quantification of DRG in rat plasma using heavy isotope labelled RES as an internal standard. The ESI was operated in its negative ion mode while DRG and RES were determined by multiple reaction monitoring (MRM) using precursor-to-product ion transitions of m/z 241...
February 13, 2018: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/29471223/indirect-calculation-of-monoclonal-antibodies-in-nanoparticles-using-the-radiolabeling-process-with-technetium-99-metastable-as-primary-factor-alternative-methodology-for-the-entrapment-efficiency
#4
Edward Helal-Neto, Santiago Sánchez Cabezas, Félix Sancenón, Ramón Martínez-Máñez, Ralph Santos-Oliveira
The use of monoclonal antibodies (Mab) in the current medicine is increasing. Antibody-drug conjugates (ADCs) represents an increasingly and important modality for treating several types of cancer. In this area, the use of Mab associated with nanoparticles is a valuable strategy. However, the methodology used to calculate the Mab entrapment, efficiency and content is extremely expensive. In this study we developed and tested a novel very simple one-step methodology to calculate monoclonal antibody entrapment in mesoporous silica (with magnetic core) nanoparticles using the radiolabeling process as primary methodology...
February 13, 2018: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/29471120/the-4-n-acyl-and-4-n-alkyl-gemcitabine-analogues-with-silicon-fluoride-acceptor-application-to-18-f-radiolabeling
#5
Cesar Gonzalez, Andersson Sanchez, Jeffrey Collins, Ksenia Lisova, Jason T Lee, R Michael van Dam, M Alejandro Barbieri, Cheppail Ramachandran, Stanislaw F Wnuk
The coupling of gemcitabine with functionalized carboxylic acids using peptide coupling conditions afforded 4-N-alkanoyl analogues with a terminal alkyne or azido moiety. Reaction of 4-N-tosylgemcitabine with azidoalkyl amine provided 4-N-alkyl gemcitabine with a terminal azido group. Click reaction with silane building blocks afforded 4-N-alkanoyl or 4-N-alkyl gemcitabine analogues suitable for fluorination. RP-HPLC analysis indicated better chemical stability of 4-N-alkyl gemcitabine analogues versus 4-N-alkanoyl analogues in acidic aqueous conditions...
February 12, 2018: European Journal of Medicinal Chemistry
https://www.readbyqxmd.com/read/29471114/reference-size-matching-whole-genome-amplification-and-fluorescent-labeling-as-a-method-for-chromosomal-microarray-analysis-of-clinically-actionable-copy-number-alterations-in-formalin-fixed-paraffin-embedded-tumor-tissue
#6
Shelly R Gunn, Shailin Govender, Cynthe L Sims, Aditi Khurana, Samuel Koo, Jayne Scoggin, Mathew W Moore, Philip D Cotter
Cancer genome copy number alterations (CNAs) assist clinicians in selecting targeted therapeutics. Solid tumor CNAs are most commonly evaluated in formalin-fixed, paraffin-embedded (FFPE) tissue by fluorescence in situ hybridization. Although fluorescence in situ hybridization is a sensitive and specific assay for interrogating pre-selected genomic regions, it provides no information about co-existing clinically significant copy number changes. Chromosomal microarray analysis is an alternative DNA-based method for interrogating genome-wide CNAs in solid tumors...
February 19, 2018: Journal of Molecular Diagnostics: JMD
https://www.readbyqxmd.com/read/29471101/on-object-selectivity-and-the-anatomy-of-the-human-fusiform-gyrus
#7
Kevin S Weiner, Vaidehi S Natu, Kalanit Grill-Spector
pFs is a functionally-defined region in the human brain that is involved in recognizing objects. A recent trend refers to pFs as the posterior fusiform sulcus, which is a neuroanatomical structure that does not exist. Here, we correct this mistake. To achieve this goal, we first recount the original definitions of pFs and then review the identification of sulci within and surrounding the fusiform gyrus (FG) including the mid-fusiform sulcus (MFS), which is a tertiary sulcus within the FG. We highlight that tertiary sulci, such as the MFS, are often absent from brain atlases, which complicates the accurate localization of functional regions, as well as the understanding of structural-functional relationships in ventral temporal cortex (VTC)...
February 19, 2018: NeuroImage
https://www.readbyqxmd.com/read/29470987/angiotensin-ii-regulates-dosing-time-dependent-intratumoral-accumulation-of-macromolecular-drug-formulations-via-24-h-blood-pressure-rhythm-in-tumor-bearing-mice
#8
Takashi Matsunaga, Naoya Matsunaga, Naoki Kusunose, Eriko Ikeda, Hiroyuki Okazaki, Keisuke Kakimoto, Kengo Hamamura, Satoru Koyanagi, Shigehiro Ohdo
One approach to increasing pharmacotherapy effects is administering drugs at times of day when they are most effective and/or best tolerated. Circadian variation in expression of pharmacokinetics- and pharmacodynamics-related genes was shown to contribute to dosing time-dependent differences in therapeutic effects of small molecule drugs. However, influence of dosing time of day on effects of high molecular weight formulations, such as drugs encapsulated in liposomes, has not been studied in detail. This study demonstrates that blood pressure rhythm affects dosing time-dependent variation in effects of high molecular weight formulations...
February 19, 2018: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29470970/comparisons-between-histology-and-optical-coherence-tomography-angiography-of-the-periarterial-capillary-free-zone
#9
Chandrakumar Balaratnasingam, Dong An, Yoichi Sakurada, Cecilia S Lee, Aaron Y Lee, Ian L McAllister, K Bailey Freund, Marinko Sarunic, Dao-Yi Yu
PURPOSE: To use the capillary-free zone along retinal arteries, a physiologic area of superficial avascularization, as an anatomic paradigm to investigate the reliability of optical coherence tomographic angiography (OCTA) for visualizing the deep retinal circulation. DESIGN: Validity analysis and laboratory investigation. METHODS: Five normal human donor eyes (mean age 69.8 years) were perfusion-labelled with endothelial antibodies and the capillary networks of the perifovea were visualized using confocal scanning laser microscopy...
February 19, 2018: American Journal of Ophthalmology
https://www.readbyqxmd.com/read/29470933/fluorescein-labeled-leukocytes-for-in-vivo-imaging-of-retinal-vascular-inflammation-and-infiltrating-leukocytes-in-laser-induced-choroidal-neovascularization-model
#10
Rupesh Agrawal, Sai Bo Bo Tun, Praveen Kumar Balne, Hong-Yuan Zhu, Neha Khandelwal, Veluchamy A Barathi
PURPOSE: To study the effect of anti-VEGF treatment on retinal inflammation in a laser-induced CNV rodent model. METHODS: Leukocytes labeled with 1% sodium fluorescein were injected into the laser-induced CNV (wild type C57BL/6) mice at days 4 (baseline), 7, 14, and 19. At baseline intravitreally 3 mice received 1× PBS, and 3 mice received anti-VEGF. FFA, OCT, and SLO were performed at each time point to assess the CNV pathophysiology and inflammatory response...
February 22, 2018: Ocular Immunology and Inflammation
https://www.readbyqxmd.com/read/29470856/leishmania-donovani-inhibits-ferroportin-translation-by-modulating-fbxl5-irp2-axis-for-its-growth-within-host-macrophages
#11
Nupur Kanti Das, Sandhya Sandhya, G Vishnu Vivek, Rajiv Kumar, Amit Kumar Singh, Saswat Kumar Bal, Sanju Kumari, Chinmay K Mukhopadhyay
Hepcidin mediated ferroportin (Fpn) degradation in macrophages is a well adopted strategy to limit iron availability towards invading pathogens. Leishmania donovani (LD), a protozoan parasite, resides within macrophage and competes with host for availing iron. Using in vitro and in vivo model of infection we reveal that LD decreases Fpn abundance in host macrophages by hepcidin independent mechanism. Unaffected level of Fpn-FLAG in LD infected J774 macrophage confirms that Fpn down-regulation is not due its degradation...
February 22, 2018: Cellular Microbiology
https://www.readbyqxmd.com/read/29470839/embryonic-neurogenesis-in-echinoderms
#12
REVIEW
Veronica F Hinman, Robert D Burke
The phylogenetic position of echinoderms is well suited to revealing shared features of deuterostomes that distinguish them from other bilaterians. Although echinoderm neurobiology remains understudied, genomic resources, molecular methods, and systems approaches have enabled progress in understanding mechanisms of embryonic neurogenesis. Even though the morphology of echinoderm larvae is diverse, larval nervous systems, which arise during gastrulation, have numerous similarities in their organization. Diverse neural subtypes and specialized sensory neurons have been identified and details of neuroanatomy using neuron-specific labels provide hypotheses for neural function...
February 22, 2018: Wiley Interdisciplinary Reviews. Developmental Biology
https://www.readbyqxmd.com/read/29470731/sacral-neuromodulation-versus-personalized-conservative-treatment-in-patients-with-idiopathic-slow-transit-constipation-study-protocol-of-the-no-2-trial-a-multicenter-open-label-randomized-controlled-trial-and-cost-effectiveness-analysis
#13
S C M Heemskerk, A H Rotteveel, M A Benninga, C I M Baeten, A A M Masclee, J Melenhorst, S M J van Kuijk, C D Dirksen, S O Breukink
PURPOSE: The evidence regarding the (cost-)effectiveness of sacral neuromodulation (SNM) in patients with therapy-resistant idiopathic slow-transit constipation is of suboptimal quality. The Dutch Ministry of Health, Welfare and Sports has granted conditional reimbursement for SNM treatment. The objective is to assess the effectiveness, cost-effectiveness, and budget impact of SNM compared to personalized conservative treatment (PCT) in patients with idiopathic slow-transit constipation refractory to conservative treatment...
February 22, 2018: International Journal of Colorectal Disease
https://www.readbyqxmd.com/read/29470705/the-effects-of-high-dose-calcitriol-and-individualized-exercise-on-bone-metabolism-in-breast-cancer-survivors-on-hormonal-therapy-a-phase-ii-feasibility-trial
#14
Luke J Peppone, Marilyn Ling, Alissa J Huston, Mary E Reid, Michelle C Janelsins, J Edward Puzas, Charles Kamen, Auro Del Giglio, Matthew Asare, Anita R Peoples, Karen M Mustian
INTRODUCTION: Cancer treatment-induced bone loss (CTIBL) is a long-term side effect of breast cancer therapy. Both calcitriol and weight-bearing exercise improve bone metabolism for osteoporotic patients, but are unproven in a breast cancer population. We used a novel high-dose calcitriol regimen with an individualized exercise intervention to improve bone metabolism in breast cancer survivors. METHODS: We accrued 41 subjects to this open label, 2 × 2 factorial, randomized feasibility trial...
February 22, 2018: Supportive Care in Cancer: Official Journal of the Multinational Association of Supportive Care in Cancer
https://www.readbyqxmd.com/read/29470663/miniaturized-dispersive-liquid-liquid-microextraction-and-maldi-ms-using-ionic-liquid-matrices-for-the-detection-of-bacterial-communication-molecules-and-virulence-factors
#15
Jan Leipert, Ingrid Bobis, Sabine Schubert, Helmut Fickenscher, Matthias Leippe, Andreas Tholey
The identification and quantification of molecules involved in bacterial communication are major prerequisites for the understanding of interspecies interactions at the molecular level. We developed a procedure allowing the determination of 2-heptyl-4(1H)-quinolone (HHQ) and 2-heptyl-3-hydroxy-4(1H)-quinolone (PQS) and the virulence factor pyocyanin (PYO) formed by the Gram-negative bacterium Pseudomonas aeruginosa. The method is based on dispersive liquid-liquid microextraction from small supernatant volumes (below 10 μL) followed by quantitative matrix-assisted laser desorption/ionization (MALDI) mass spectrometry (MS)...
February 22, 2018: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/29470308/efficacy-and-safety-outcomes-of-intravitreal-aflibercept-focusing-on-patients-with-diabetic-macular-edema-from-japan
#16
Hiroko Terasaki, Kunihiko Shiraki, Masahito Ohji, Carola Metzig, Thomas Schmelter, Oliver Zeitz, Olaf Sowade, Masato Kobayashi, Robert Vitti, Alyson Berliner, Fumio Shiraga
PURPOSE: To evaluate the efficacy and safety of intravitreal aflibercept injection (IAI) in Japanese patients with diabetic macular edema (DME). METHODS: VIVID-DME was a Phase 3 study comprising patients with DME randomized 1:1:1 to IAI 2 mg every 4 weeks (2q4), IAI 2 mg every 4 weeks until Week 16 then 8-week dosing (2q8), and laser. A total of 403 patients (76 Japanese) were included in this study. VIVID-Japan (72; all Japanese patients) was a nonrandomized, open-label study comprising Japanese patients with DME receiving IAI 2q4 until Week 16, then 2q8...
February 21, 2018: Retina
https://www.readbyqxmd.com/read/29470192/sensitivity-of-a-reduced-eeg-montage-for-seizure-detection-in-the-neurocritical-care-setting
#17
Brandy B Ma, Emily L Johnson, Eva K Ritzl
INTRODUCTION: Neurocritical care units commonly implement the double-distance reduced EEG montage in postoperative neurosurgic patients who have structural barriers that hinder the placement of a standard 10-20 system array. Despite its widespread use, its sensitivity has not been adequately addressed. We evaluated the sensitivity and specificity of this montage for seizure detection. METHODS: One hundred fifty-five full-montage continuous EEGs (cEEGs) completed in the Johns Hopkins University neurocritical care unit containing unequivocal electrographic seizures, status epilepticus, or other abnormalities were selected, comprising 73 ictal and 82 nonictal EEGs...
February 21, 2018: Journal of Clinical Neurophysiology: Official Publication of the American Electroencephalographic Society
https://www.readbyqxmd.com/read/29470171/hyperspectral-image-classification-with-markov-random-fields-and-a-convolutional-neural-network
#18
Xiangyong Cao, Feng Zhou, Lin Xu, Deyu Meng, Zongben Xu, John Paisley
This paper presents a new supervised classification algorithm for remotely sensed hyperspectral image (HSI) which integrates spectral and spatial information in a unified Bayesian framework. First, we formulate the HSI classification problem from a Bayesian perspective. Then, we adopt a convolutional neural network (CNN) to learn the posterior class distributions using a patch-wise training strategy to better use the spatial information. Next, spatial information is further considered by placing a spatial smoothness prior on the labels...
May 2018: IEEE Transactions on Image Processing: a Publication of the IEEE Signal Processing Society
https://www.readbyqxmd.com/read/29470159/central-metatarsalgia-and-walking-on-pebbles-beyond-morton-neuroma
#19
Akash Ganguly, Joanne Warner, Hifz Aniq
OBJECTIVE: Central metatarsalgia relates to abnormalities of the second, third, and fourth metatarsals and their respective metatarsophalangeal joints. A variety of disorders present with central forefoot pain; they range from traumatic lesions (acute or chronic repetitive), inflammatory and infective disorders, nonneoplastic soft-tissue lesions, and benign tumors to malignant lesions. Patients often present with symptoms of localized pain in the forefoot that worsens on weight bearing (walking or running), which can be sharp or dull and often is perceived as a lump felt inside or underneath the foot and described as walking on a marble or pebbles...
February 22, 2018: AJR. American Journal of Roentgenology
https://www.readbyqxmd.com/read/29470156/journal-club-evaluation-of-diffusion-kurtosis-imaging-of-stroke-lesion-with-hemodynamic-and-metabolic-mri-in-a-rodent-model-of-acute-stroke
#20
Dongshuang Lu, Yinghua Jiang, Yang Ji, Iris Yuwen Zhou, Emiri Mandeville, Eng H Lo, Xiaoying Wang, Phillip Zhe Sun
OBJECTIVE: Diffusion kurtosis imaging (DKI) has emerged as a new acute stroke imaging approach, augmenting routine DWI. Although it has been shown that a diffusion lesion without kurtosis abnormality is more likely to recover after reperfusion, whereas a kurtosis lesion shows poor response, little is known about the underlying pathophysiologic profile of the kurtosis lesion versus the kurtosis lesion-diffusion lesion mismatch. MATERIALS AND METHODS: We performed multiparametric MRI, including arterial spin labeling, pH-sensitive amide proton transfer, and DKI, in a rodent model of acute stroke caused by embolic middle cerebral artery occlusion...
February 22, 2018: AJR. American Journal of Roentgenology
keyword
keyword
37579
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"