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https://www.readbyqxmd.com/read/29036196/understanding-the-antimicrobial-activity-of-selected-disinfectants-against-methicillin-resistant-staphylococcus-aureus-mrsa
#1
Ebrahim Aboualizadeh, Violet V Bumah, Daniela S Masson-Meyers, Janis T Eells, Carol J Hirschmugl, Chukuka S Enwemeka
Disinfectants and biocidal products have been widely used to combat Methicillin-resistant Staphylococcus aureus (MRSA) infections in homes and healthcare environments. Although disruption of cytoplasmic membrane integrity has been documented as the main bactericidal effect of biocides, little is known about the biochemical alterations induced by these chemical agents. In this study, we used Fourier transform infrared (FT-IR) spectroscopy and chemometric tools as an alternative non-destructive technique to determine the bactericidal effects of commonly used disinfectants against MRSA USA-300...
2017: PloS One
https://www.readbyqxmd.com/read/29034862/ultrastructural-and-microbial-analyses-of-cellulose-degradation-in-leaf-cutter-ant-colonies
#2
Rolando Daniel Moreira-Soto, Ethel Sanchez, Cameron R Currie, Adrian A Pinto-Tomás
Leaf-cutter ants (Atta and Acromyrmex) use fresh leaves to cultivate a mutualistic fungus (Leucoagaricus gongylophorus) for food in underground gardens. A new ant queen propagates the cultivar by taking a small fragment of fungus from her parent colony on her nuptial flight and uses it to begin her own colony. Recent research has shown that the ants' fungus gardens are colonized by symbiotic bacteria that perform important functions related to nitrogen fixation and have been implicated in contributing to plant biomass degradation...
October 16, 2017: Microbiology
https://www.readbyqxmd.com/read/29034439/low-grade-small-intestinal-bacterial-overgrowth-is-common-in-patients-with-non-alcoholic-steatohepatitis-on-quantitative-jejunal-aspirate-culture
#3
Uday C Ghoshal, Chalamalasetty Sreenivasa Baba, Ujjala Ghoshal, George Alexander, Asha Misra, Vivek A Saraswat, Gourdas Choudhuri
BACKGROUND: Though pathogenesis of non-alcoholic steatohepatitis (NASH) is unclear, association with small intestinal bacterial overgrowth [SIBO] and fecal dysbiosis is suggested. We evaluated SIBO in NASH using quantitative jejunal aspirate culture (conventional criteria: ≥ 10(5) colony forming unit (CFU)/mL and newer cutoff ≥ 10(3) CFU/mL) and glucose hydrogen breath test. METHODS: Thirty-eight patients with NASH (age 37.5 years, range 20-54, 9, 24% female), diagnosed by ultrasonography, alanine aminotransferase >1...
October 16, 2017: Indian Journal of Gastroenterology: Official Journal of the Indian Society of Gastroenterology
https://www.readbyqxmd.com/read/29033917/newly-isolated-bacteriophages-from-the-podoviridae-siphoviridae-and-myoviridae-families-have-variable-effects-on-putative-novel-dickeya-spp
#4
Špela Alič, Tina Naglič, Magda Tušek-Žnidarič, Maja Ravnikar, Nejc Rački, Matjaž Peterka, Tanja Dreo
Soft rot pathogenic bacteria from the genus Dickeya cause severe economic losses in orchid nurseries worldwide, and there is no effective control currently available. In the last decade, the genus Dickeya has undergone multiple changes as multiple new taxa have been described, and just recently a new putative Dickeya species was reported. This study reports the isolation of three bacteriophages active against putative novel Dickeya spp. isolates from commercially produced infected orchids that show variable host-range profiles...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29032514/surface-roughness-and-bacterial-adhesion-on-root-dentin-treated-with-diode-laser-and-conventional-desensitizing-agents
#5
Maiza S Cury, Camilla B Silva, Ruchele D Nogueira, Michelle G D Campos, Regina G Palma-Dibb, Vinicius R Geraldo-Martins
The treatments for dentin hypersensitivity (DH) may change the surface roughness of the root dentin, which can lead to biofilm accumulation, increasing the risk of root caries. The aim was to compare the surface roughness of root dentin after different treatments of DH and the biofilm formation on those surfaces. After initial surface roughness (Sa) assessment, 50 bovine root fragments received the following treatments (n = 10): G 1-no treatment; G2-5% sodium fluoride varnish; G3-professional application of a desensitizing dentifrice; G4-toothbrushing with a desensitizing dentifrice; and G5-diode laser application (908 nm; 1...
October 14, 2017: Lasers in Medical Science
https://www.readbyqxmd.com/read/29032472/application-of-proteotyping-strain-solution%C3%A2-ver-2-software-and-theoretically-calculated-mass-database-in-maldi-tof-ms-typing-of-salmonella-serotype
#6
Teruyo Ojima-Kato, Naomi Yamamoto, Satomi Nagai, Keisuke Shima, Yumi Akiyama, Junji Ota, Hiroto Tamura
Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS)-based microbial identification is a popular analytical method. Strain Solution proteotyping software available for MALDI-TOF MS has great potential for the precise and detailed discrimination of microorganisms at serotype- or strain-level, beyond the conventional mass fingerprinting approaches. Here, we constructed a theoretically calculated mass database of Salmonella enterica subspecies enterica consisting of 12 biomarker proteins: ribosomal proteins S8, L15, L17, L21, L25, and S7, Mn-cofactor-containing superoxide dismutase (SodA), peptidyl-prolyl cis-trans isomerase C (PPIase C), and protein Gns, and uncharacterized proteins YibT, YaiA, and YciF, that can allow serotyping of Salmonella...
October 14, 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/29032227/effectiveness-of-photodynamic-therapy-associated-with-irrigants-over-two-biofilm-models
#7
Ricardo Abreu da Rosa, Manuela Favarin Santini, José Antônio Poli de Figueiredo, Fernanda Visioli, Jefferson Ricardo Pereira, Rodrigo Ricci Vivan, Francisco Montagner, Marcus Vinícius Reis Só
BACKGROUND: This study aimed to evaluate the antibacterial effect and the biofilm disruption promoted by antimicrobial photodynamic therapy (aPDT) associated with sodium hypochlorite (NaOCl) and chlorexidine (CHX) over monospecies and multispecies biofilms. METHODS: In monospecies model, forty-six premolars were inoculated with Enterococcus faecalis for 21days and divided into three groups: saline, CHX and NaOCl. After irrigation, aPDT was performed. Samples were collected at baseline (S1) and after irrigation (S2) and aPDT (S3)...
October 12, 2017: Photodiagnosis and Photodynamic Therapy
https://www.readbyqxmd.com/read/29029221/analysis-of-clostridium-difficile-biofilms-imaging-and-antimicrobial-treatment
#8
Garth A James, Laurent Chesnel, Laura Boegli, Elinor deLancey Pulcini, Steve Fisher, Philip S Stewart
Background: Clostridium difficile, a spore-forming Gram-positive anaerobic bacillus, is the most common causative agent of healthcare-associated diarrhoea. Formation of biofilms may protect C. difficile against antibiotics, potentially leading to treatment failure. Furthermore, bacterial spores or vegetative cells may linger in biofilms in the gut causing C. difficile infection recurrence. Objectives: In this study, we evaluated and compared the efficacy of four antibiotics (fidaxomicin, surotomycin, vancomycin and metronidazole) in penetrating C...
October 6, 2017: Journal of Antimicrobial Chemotherapy
https://www.readbyqxmd.com/read/29029218/dose-dependent-effects-on-replication-and-persistence-of-salmonella-enterica-serovar-typhimurium-in-house-flies-diptera-muscidae
#9
Rabecca Chifanzwa, Dana Nayduch
Adult house flies (Musca domestica L.) ingest variable numbers of bacteria when they encounter microbe-rich substrates. Bacterial abundance may affect replication within the fly gut, which subsequently impacts vector potential. This study investigated the dose-dependent replication of GFP-expressing Salmonella enterica serovar Typhimurium (ex Kauffmann and Edwards1952) Le Minor and Popoff 1987, (Enterobacteriales: Enterobacteriaceae) (GFP S. Typhimurium) within the fly alimentary canal. Adult house flies were fed two doses (colony forming units, CFU) of GFP S...
October 4, 2017: Journal of Medical Entomology
https://www.readbyqxmd.com/read/29028576/bioelectrochromic-hydrogel-for-fast-antibiotic-susceptibility-testing
#10
Ferran Pujol-Vila, Jiri Dietvorst, Laura Gall-Mas, María Díaz-González, Núria Vigués, Jordi Mas, Xavier Muñoz-Berbel
Materials science offers new perspectives in the clinical analysis of antimicrobial sensitivity. However, a biomaterial with the capacity to respond to living bacteria has not been developed to date. We present an electrochromic iron(III)-complexed alginate hydrogel sensitive to bacterial metabolism, here applied to fast antibiotic-susceptibility determination. Bacteria under evaluation are entrapped -and pre-concentrated- in the hydrogel matrix by oxidation of iron (II) ions to iron (III) and in situ formation of the alginate hydrogel in less than 2min and in soft experimental conditions (i...
September 6, 2017: Journal of Colloid and Interface Science
https://www.readbyqxmd.com/read/29026702/apical-extrusion-of-intracanal-bacteria-with-single-file-and-multifile-rotary-instrumentation-systems
#11
Eshaghali Saberi, Shahram Shahraki Zahedani, Sediqe Ebrahimipour
AIMS AND OBJECTIVES: Instrumentation techniques may cause extrusion of microorganisms and their products into the periapical region resulting inflammation and treatment failure. The aim of this ex vivo study was comparing the apical bacterial extrusion in canals prepared with single file versus multiple file rotary systems. MATERIALS AND METHODS: Ninety-two human single-rooted mandibular first premolars were used. Endodontic access cavities were prepared, and root canals were contaminated with an Enterococcus faecalis (E...
September 2017: Journal of International Society of Preventive & Community Dentistry
https://www.readbyqxmd.com/read/29026620/streptococcus-suis-serotype-9-endocarditis-and-subsequent-severe-meningitis-in-a-growing-pig-despite-specific-bactericidal-humoral-immunity
#12
Karoline Rieckmann, Kristin Müller, Annette Moter, Christoph G Baums, Anna Seydel
INTRODUCTION: . Meningitis and endocarditis are common pathologies of Streptococcussuis infections in pigs and humans. S. suis serotype 9 strains contribute substantially to health problems in European pig production, and immune prophylaxis against this serotype is very difficult. CASE PRESENTATION: . We report the clinical course and histopathological picture of a 10-week-old growing pig following experimental intravenous infection with S. suis serotype 9. The piglet showed rapid onset of severe clinical signs of meningitis 11 days post-intravenous challenge following prime-booster vaccination...
May 2017: JMM Case Reports
https://www.readbyqxmd.com/read/29022742/culture-based-quantification-with-molecular-characterization-of-non-o157-and-o157-enterohemorrhagic-escherichia-coli-isolates-from-rectoanal-mucosal-swabs-of-feedlot-cattle
#13
Zachary R Stromberg, Gentry L Lewis, Liesel G Schneider, Galen E Erickson, Isha R Patel, David R Smith, Rodney A Moxley
Enterohemorrhagic Escherichia coli (EHEC) strains are foodborne pathogens carried in the intestinal tracts of ruminants and shed in the feces. High concentrations (≥10(4) colony-forming units [CFU]/g) of EHEC in cattle feces are associated with contamination of hides, and subsequently, carcasses and beef. Several studies using agar media have quantified O157 but few have quantified non-O157 EHEC in samples from cattle. Thus, the objective of this study was to determine the concentration of O157 and non-O157 EHEC in cattle, and to characterize the associated EHEC isolates for their virulence potential...
October 12, 2017: Foodborne Pathogens and Disease
https://www.readbyqxmd.com/read/29021709/subacute-oral-toxicity-and-bacterial-mutagenicity-study-of-korean-red-ginseng-oil
#14
Hwi Won Seo, Jae Hyun Suh, Seung-Ho So, Jong-Soo Kyung, Yong-Soon Kim, Chang-Kyun Han
BACKGROUND: Red ginseng oil (RGO) is produced by supercritical CO2 extraction of secondary products derived from Korean Red Ginseng extract. As the use of RGO has increased, product safety concerns have become more important. METHODS: In the present study, the subacute oral toxicity and bacterial reverse mutagenicity of RGO were evaluated. Sprague-Dawley rats were orally administered with RGO for 28 d by gavage. Daily RGO dose concentrations were 0 mg/kg body weight (bw), 500 mg/kg bw, 1,000 mg/kg bw, or 2,000 mg/kg bw per day...
October 2017: Journal of Ginseng Research
https://www.readbyqxmd.com/read/29021487/severe-infection-of-pseudomonas-aeruginosa-during-eculizumab-therapy-for-paroxysmal-nocturnal-hemoglobinuria
#15
Toru Kawakami, Hideyuki Nakazawa, Yukifumi Kurasawa, Hitoshi Sakai, Sayaka Nishina, Noriko Senoo, Yasushi Senoo, Fumihiro Ishida
Eculizumab is the complement inhibitor administered to ameliorate intravascular hemolysis in paroxysmal nocturnal hemoglobinuria (PNH). Whether or not the inhibitory mechanism may also increase the susceptibility to non-Neisserial infection is unclear. A 73-year old woman presented with bacteremia, cholecystitis and liver abscess with Pseudomonas aeruginosa. Although she had been neutropenic for 21 years, she had no history of severe infection before eculizumab had been administered. The infection with P. aeruginosa was successfully controlled with antibiotics, granulocyte colony-stimulating factor and cholecystectomy...
October 11, 2017: Internal Medicine
https://www.readbyqxmd.com/read/29021155/effects-of-clinically-meaningful-concentrations-of-anti-pseudomonal-%C3%AE-lactams-on-time-to-detection-and-organism-growth-in-blood-culture-bottles
#16
Mordechai Grupper, David P Nicolau, Jaber Aslanzadeh, Linda K Tanner, Joseph L Kuti
The effectiveness of antimicrobial binding resins present in blood culture (BC) bottles in removing meropenem, ceftolozane/tazobactam, and ceftazidime/avibactam is unknown. We assessed the time to detection (TTD) and growth of 2 Pseudomonas aeruginosa isolates in the presence of clinically meaningful concentrations of these antibiotics. BACTEC Plus Aerobic/F and BacT/ALERT FA Plus BC bottles were inoculated with one of two isolates (1 meropenem susceptible, 1 resistant), followed by fresh whole blood containing the peak, midpoint, or trough plasma concentrations for meropenem, ceftolozane/tazobactam, and ceftazidime/avibactam...
October 11, 2017: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/29020098/treatment-with-high-dose-antidepressants-severely-exacerbates-the-pathological-outcome-of-experimental-escherichia-coli-infections-in-poultry
#17
Sofie Kromann, Egle Kudirkiene, Lili Li, Ida Thoefner, Elisabeth Daldorph, Jens Peter Christensen, Hecheng Meng, Rikke Heidemann Olsen
There is an urgent need for novel antibiotics as the current antibiotics are losing their value due to increased resistance among clinically important bacteria. Sertraline, an on-marked anti-depressive drug, has been shown to modify bacterial activity in vitro, including increasing the susceptibility of Escherichia coli to antibiotics. The aim of the present study was to investigate if the antimicrobial activity of sertraline could be documented under clinical settings, hereunder if sertraline could potentiate the effect of tetracycline in treatment of an experimentally induced ascending infection in poultry...
2017: PloS One
https://www.readbyqxmd.com/read/29018421/himar1-transposon-for-efficient-random-mutagenesis-in-aggregatibacter-actinomycetemcomitans
#18
Qinfeng Ding, Kai Soo Tan
Aggregatibacter actinomycetemcomitans is the primary etiological agent of aggressive periodontal disease. Identification of novel virulence factors at the genome-wide level is hindered by lack of efficient genetic tools to perform mutagenesis in this organism. The Himar1 mariner transposon is known to yield a random distribution of insertions in an organism's genome with requirement for only a TA dinucleotide target and is independent of host-specific factors. However, the utility of this system in A. actinomycetemcomitans is unknown...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29016585/isolation-of-multidrug-resistant-bacteria-from-patients-medical-charts
#19
Roshan Thapa, Narayan Dutt Pant, Uday Narayan Yadav, Eliza Thapa, Anjana Singh, Bharat Mani Pokhrel, Upendra Devkota
BACKGROUND: Patient's medical charts in hospitals are potentially contaminated by pathogenic bacteria and might act as vehicles for transmission of bacterial infections.This study was aimed to determine the rate of contamination of medical charts by multidrug resistant bacteria. METHODS: Sampling of total 250 patient's medical charts from different wards was done with the help of cotton swabs soaked in sterile normal saline. The swabs thus collected were cultured using standard microbiological procedures...
September 8, 2017: Journal of Nepal Health Research Council
https://www.readbyqxmd.com/read/28994811/precise-high-throughput-analysis-of-bacterial-growth
#20
Masaomi Kurokawa, Bei-Wen Ying
Bacterial growth is a central concept in the development of modern microbial physiology, as well as in the investigation of cellular dynamics at the systems level. Recent studies have reported correlations between bacterial growth and genome-wide events, such as genome reduction and transcriptome reorganization. Correctly analyzing bacterial growth is crucial for understanding the growth-dependent coordination of gene functions and cellular components. Accordingly, the precise quantitative evaluation of bacterial growth in a high-throughput manner is required...
September 19, 2017: Journal of Visualized Experiments: JoVE
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