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https://www.readbyqxmd.com/read/28439257/high-resolution-recording-of-the-circadian-oscillator-in-primary-mouse-%C3%AE-and-%C3%AE-cell-culture
#1
Volodymyr Petrenko, Yvan Gosmain, Charna Dibner
Circadian clocks have been developed in evolution as an anticipatory mechanism allowing for adaptation to the constantly changing light environment due to rotation of the Earth. This mechanism is functional in all light-sensitive organisms. There is a considerable body of evidence on the tight connection between the circadian clock and most aspects of physiology and metabolism. Clocks, operative in the pancreatic islets, have caught particular attention in the last years due to recent reports on their critical roles in regulation of insulin secretion and etiology of type 2 diabetes...
2017: Frontiers in Endocrinology
https://www.readbyqxmd.com/read/28430754/biomarkers-for-early-acute-kidney-injury-diagnosis-and-severity-prediction-a-pilot-multicenter-canadian-study-of-children-admitted-to-the-icu
#2
Jennifer Palermo, Allison B Dart, Alanna De Mello, Prasad Devarajan, Ronald Gottesman, Gonzalo Garcia Guerra, Greg Hansen, Ari R Joffe, Cherry Mammen, Nick Majesic, Catherine Morgan, Peter Skippen, Michael Pizzi, Ana Palijan, Michael Zappitelli
OBJECTIVE: Acute kidney injury occurs early in PICU admission and increases risks for poor outcomes. We evaluated the feasibility of a multicenter acute kidney injury biomarker urine collection protocol and measured diagnostic characteristics of urine neutrophil gelatinase-associated lipocalin, interleukin-18, and liver fatty acid binding protein to predict acute kidney injury and prolonged acute kidney injury. DESIGN: Prospective observational pilot cohort study...
April 20, 2017: Pediatric Critical Care Medicine
https://www.readbyqxmd.com/read/28430735/sodium-tetradecyl-sulfate-a-review-of-clinical-uses
#3
Helena A Jenkinson, Kelly M Wilmas, Sirunya Silapunt
BACKGROUND: Sodium tetradecyl sulfate (STS) is Food and Drug Administration approved for treatment of varicose veins, but numerous other off-label applications have been reported. OBJECTIVE: To describe the clinical uses of STS, as well as efficacy and adverse effects. METHODS: Review of studies searchable on PubMed from 1938 to 2016 describing clinical uses of STS to determine efficacy and adverse effects associated with various applications...
April 19, 2017: Dermatologic Surgery: Official Publication for American Society for Dermatologic Surgery [et Al.]
https://www.readbyqxmd.com/read/28425501/plant-gum-identification-in-historic-artworks
#4
Clara Granzotto, Julie Arslanoglu, Christian Rolando, Caroline Tokarski
We describe an integrated and straightforward new analytical protocol that identifies plant gums from various sample sources including cultural heritage. Our approach is based on the identification of saccharidic fingerprints using mass spectrometry following controlled enzymatic hydrolysis. We developed an enzyme cocktail suitable for plant gums of unknown composition. Distinctive MS profiles of gums such as arabic, cherry and locust-bean gums were successfully identified. A wide range of oligosaccharidic combinations of pentose, hexose, deoxyhexose and hexuronic acid were accurately identified in gum arabic whereas cherry and locust bean gums showed respectively PentxHexy and Hexn profiles...
April 20, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28425087/peer-connect-for-african-american-breast-cancer-survivors-and-caregivers-a-train-the-trainer-approach-for-peer-support
#5
Marlyn Allicock, Lindsey Haynes-Maslow, La-Shell Johnson, William R Carpenter, Anissa I Vines, Denise G Belle, Ray Phillips, Michele W Cherry
Racial disparities in breast cancer survivorship are a major concern nationally. How survivors cope with cancer and re-frame their lives is a critical part of survivorship. Community-academic research partnerships may facilitate access to much-needed psychosocial support for African American survivors and caregivers in rural areas, but drivers of successful intervention implementation are not well understood. The purpose of this study was to describe the training and evaluation of Community Coaches and Guides (i...
April 19, 2017: Translational Behavioral Medicine
https://www.readbyqxmd.com/read/28420222/how-sensors-might-help-define-the-external-exposome
#6
REVIEW
Miranda Loh, Dimosthenis Sarigiannis, Alberto Gotti, Spyros Karakitsios, Anjoeka Pronk, Eelco Kuijpers, Isabella Annesi-Maesano, Nour Baiz, Joana Madureira, Eduardo Oliveira Fernandes, Michael Jerrett, John W Cherrie
The advent of the exposome concept, the advancement of mobile technology, sensors, and the "internet of things" bring exciting opportunities to exposure science. Smartphone apps, wireless devices, the downsizing of monitoring technologies, along with lower costs for such equipment makes it possible for various aspects of exposure to be measured more easily and frequently. We discuss possibilities and lay out several criteria for using smart technologies for external exposome studies. Smart technologies are evolving quickly, and while they provide great promise for advancing exposure science, many are still in developmental stages and their use in epidemiology and risk studies must be carefully considered...
April 18, 2017: International Journal of Environmental Research and Public Health
https://www.readbyqxmd.com/read/28420063/patients-experiences-of-lupus-related-foot-problems-a-qualitative-investigation
#7
A E Williams, A Blake, L Cherry, B Alcacer-Pitarch, C J Edwards, N Hopkinson, E M J Vital, L S Teh
Background Systemic lupus erythematosus (SLE) can present with a variety of symptoms. Previous research has shown there is a high prevalence of lower limb and foot problems in patients with SLE associated with the musculoskeletal, vascular and neurological changes. Furthermore, there is a high prevalence of infections affecting the feet and a range of common skin and nail problems. However, it is not known how these foot problems impact upon people's lives. Therefore, we aimed to explore this using a qualitative approach...
January 1, 2017: Lupus
https://www.readbyqxmd.com/read/28419331/an-apoplastic-%C3%AE-glucosidase-is-essential-for-the-degradation-of-flavonol-3-o-%C3%AE-glucoside-7-o-%C3%AE-rhamnosides-in-arabidopsis
#8
Jonathon Roepke, Harley O W Gordon, Kevin J A Neil, Satinder Gidda, Robert T Mullen, José A Freixas Coutin, Delaney Bray-Stone, Gale G Bozzo
Flavonol bisglycosides accumulate in plant vegetative tissues in response to abiotic stress, including simultaneous environmental perturbations (i.e., nitrogen deficiency and low temperature, NDLT), but disappear with recovery from NDLT. Previously, we determined that a recombinant Arabidopsis β-glucosidase (BGLU), BGLU15, hydrolyzes flavonol 3-O-β-glucoside-7-O-α-rhamnosides and flavonol 3-O-β-glucosides, forming flavonol 7-O-α-rhamnosides and flavonol aglycones, respectively. In this study, the transient expression of a BGLU15-Cherry fusion protein in onion epidermal cells demonstrated that BGLU15 was localized to the apoplast...
April 13, 2017: Plant & Cell Physiology
https://www.readbyqxmd.com/read/28418524/evaluating-testing-strategies-for-identifying-youths-with-hiv-infection-and-linking-youths-to-biomedical-and-other-prevention-services
#9
Robin Lin Miller, Cherrie B Boyer, Danielle Chiaramonte, Peter Lindeman, Kate Chutuape, Bendu Cooper-Walker, Bill G Kapogiannis, Craig M Wilson, J Dennis Fortenberry
Importance: Most human immunodeficiency virus (HIV)-infected youths are unaware of their serostatus (approximately 60%) and therefore not linked to HIV medical or prevention services. The need to identify promising and scalable approaches to promote uptake of HIV testing among youths at risk is critical. Objective: To evaluate a multisite HIV testing program designed to encourage localized HIV testing programs focused on self-identified sexual minority males and to link youths to appropriate prevention services after receipt of their test results...
April 17, 2017: JAMA Pediatrics
https://www.readbyqxmd.com/read/28417450/exploring-the-biological-activity-of-condensed-tannins-and-nutritional-value-of-tree-and-shrub-leaves-from-native-species-of-the-argentinean-dry-chaco
#10
E M García, N Cherry, B D Lambert, J P Muir, M A Nazareno, J I Arroquy
BACKGROUND: Tropical tree or shrub leaves are an important source of nutrients for ruminants and a potential source of biologically active compounds that may affect ruminal metabolism of nutrients. Therefore, eight woody species from the native flora of Argentinean Dry Chaco - rich in secondary compounds such as condensed tannins (CT), were assessed for their nutritional value, CT fractions, and in vitro true digestibility of dry matter as well as biological activity (BA). RESULTS: Differences among species were found in contents of total phenol, protein-precipitating phenols (PPP), bound proteins to PPP (BP) and BP/PPP (P<0...
April 18, 2017: Journal of the Science of Food and Agriculture
https://www.readbyqxmd.com/read/28417072/atypical-juvenile-presentation-of-gm2-gangliosidosis-ab-in-a-patient-compound-heterozygote-for-c-259g%C3%A2-%C3%A2-t-and-c-164c%C3%A2-%C3%A2-t-mutations-in-the-gm2a-gene
#11
Carla Martins, Catherine Brunel-Guitton, Anne Lortie, France Gauvin, Carlos R Morales, Grant A Mitchell, Alexey V Pshezhetsky
GM2-gangliosidosis, AB variant is an extremely rare autosomal recessive inherited disorder caused by mutations in the GM2A gene that encodes GM2 ganglioside activator protein (GM2AP). GM2AP is necessary for solubilisation of GM2 ganglioside in endolysosomes and its presentation to β-hexosaminidase A. Conversely GM2AP deficiency impairs lysosomal catabolism of GM2 ganglioside, leading to its storage in cells and tissues. We describe a 9-year-old child with an unusual juvenile clinical onset of GM2-gangliosidosis AB...
June 2017: Molecular Genetics and Metabolism Reports
https://www.readbyqxmd.com/read/28416865/folic-acid-content-and-antioxidant-activity-of-different-types-of-beers-available-in-hungarian-retail
#12
Dániel Koren, Csaba Orbán, Nóra Galló, Szilárd Kun, Beáta Vecseri-Hegyes, Gabriella Kun-Farkas
In this study 40 Hungarian retail beers were evaluated for folic acid content, antioxidant profile and physicochemical parameters. The physicochemical parameters, folic acid content and antioxidant activity of alcohol-free beers were the lowest. Folic acid content of beers aged with sour cherries showed high values, more than 0.4 mg/l and an alcohol-free beer-based mixed drink made with lemon juice contained more than 0.2 mg/l of folic acid. Dark beers and beers aged with sour cherries had the highest antioxidant activity probably owing to their high extract content, components released from the fruits and special malts...
April 2017: Journal of Food Science and Technology
https://www.readbyqxmd.com/read/28416490/preclinical-characterization-of-bet-family-bromodomain-inhibitor-abbv-075-suggests-combination-therapeutic-strategies
#13
Mai H Bui, Xiaoyu Lin, Daniel H Albert, Leiming Li, Lloyd T Lam, Emily J Faivre, Scott Warder, Xiaoli Huang, Denise Wilcox, Cherrie K Donawho, George S Sheppard, Le Wang, Steve Fidanze, John K Pratt, Dachun Liu, Lisa Hasvold, Tamar Uziel, Xin Lu, Fred Kohlhapp, Guowei Fang, Steven W Elmore, Saul H Rosenberg, Keith F McDaniel, Warren M Kati, Yu Shen
ABBV-075 is a potent and selective BET family bromodomain inhibitor that recently entered Phase I clinical trials. Comprehensive preclinical characterization of ABBV-075 demonstrated broad activity across cell lines and tumor models representing a variety of hematological malignancies and solid tumor indications. In most cancer cell lines derived from solid tumors, ABBV-075 triggers prominent G1 cell cycle arrest without extensive apoptosis. In this study, we show that ABBV-075 efficiently triggers apoptosis in acute myeloid leukemia (AML), non-Hodgkin's lymphoma (NHL) and multiple myeloma (MM) cells...
April 17, 2017: Cancer Research
https://www.readbyqxmd.com/read/28412858/profile-of-lipophilic-antioxidants-in-the-by-products-recovered-from-six-cultivars-of-sour-cherry-prunus-cerasus-l
#14
Vitalijs Radenkovs, Daina Feldmane
The content of tocochromanols and carotenoids in kernels recovered from fruit pits of six sour cherry (Prunus cerasus L.) cultivars was studied. The highest concentration was noted for γ-T (23.50-52.76 mg/100 g dry weight basis (dw)). Considerably, lower amounts compared to γ-T were detected for α-T, δ-T and α-T3 (5.74-13.33, 2.74-4.54 and 0.30-1.16 mg/100 g dw, respectively). The β-T and γ-T3 were quantified only in minor levels. The total amount of tocochromanols was in the range 41.69-63.48 mg/100 g dw...
April 17, 2017: Natural Product Research
https://www.readbyqxmd.com/read/28411365/analysis-of-glycosylated-flavonoids-extracted-from-sweet-cherry-stems-as-antibacterial-agents-against-pathogenic-escherichia-coli-isolates
#15
Alfredo Aires, Carla Dias, Rosa Carvalho, Maria José Saavedra
The aim of this study was to evaluate the bioactivity of flavonoids extracted from sweet-cherry stems which are often used by a traditional system of medicine to treat gastro-intestinal and urinary tract infections but lacking any consistent scientific evidence; moreover the information about the class of phenolics, their content and the potential bioactivity of such material is very scarce. Thus, in this context, we have set a research study in which we evaluated the profile and content of phenolics extracted from sweet-cherry stems through a conventional (70ºC and 20 min) and ultrasound assisted extraction (40 kHz, room temperature and 20 min)...
April 14, 2017: Acta Biochimica Polonica
https://www.readbyqxmd.com/read/28407914/effects-of-hybrid-microwave-convectional-and-convectional-drying-on-drying-kinetics-total-phenolics-antioxidant-capacity-vitamin-c-color-and-rehydration-capacity-of-sour-cherries
#16
Erhan Horuz, Hüseyin Bozkurt, Haluk Karataş, Medeni Maskan
Sour cherries were dried by convectional (CD) at 50, 60, and 70°C and by hybrid (HD; microwave-convectional) drying at 120, 150, and 180W coupled with hot air at 50, 60, and 70°C. In HD, microwave power and hot air were performed simultaneously during drying process. Total phenolic content (TPC), antioxidant capacity (AOC), vitamin C content, color and rehydration capacity were followed. The HD technique reduced drying time and increased drying rate compared to CD. Drying data were well correlated with Verma and modified Logistic models which gave the best fitting for CD and HD, respectively...
September 1, 2017: Food Chemistry
https://www.readbyqxmd.com/read/28407490/co2-effects-on-the-waterlogging-response-of-gisela-5-and-gisela-6-prunus-cerasusxprunus-canescens-sweet-cherry-prunus-avium-rootstocks
#17
Margarita Pérez-Jiménez, María Hernández-Munuera, M Carmen Piñero, Gregorio López-Ortega, Francisco M Del Amor
Climate change is submitting countries of the Mediterranean arc to periods of drought alternating with heavy rain and waterlogging. Eventual floods along with the rising CO2 in the atmosphere present an unpredictable scenario that affects crop survival. The effect of both stresses combined has been studied in sweet cherry plants. 'Gisela 5' and 'Gisela 6' were evaluated as rootstocks of the sweet cherry cultivar 'Burlat'. Plants were placed in a controlled-climate chamber for 7days, then they were submitted to waterlogging for another 7days and the response to this stress and the subsequent recovery were studied (7 more days)...
March 28, 2017: Journal of Plant Physiology
https://www.readbyqxmd.com/read/28403240/snovault-and-encoded-a-novel-object-based-storage-system-and-applications-to-encode-metadata
#18
Benjamin C Hitz, Laurence D Rowe, Nikhil R Podduturi, David I Glick, Ulugbek K Baymuradov, Venkat S Malladi, Esther T Chan, Jean M Davidson, Idan Gabdank, Aditi K Narayana, Kathrina C Onate, Jason Hilton, Marcus C Ho, Brian T Lee, Stuart R Miyasato, Timothy R Dreszer, Cricket A Sloan, J Seth Strattan, Forrest Y Tanaka, Eurie L Hong, J Michael Cherry
The Encyclopedia of DNA elements (ENCODE) project is an ongoing collaborative effort to create a comprehensive catalog of functional elements initiated shortly after the completion of the Human Genome Project. The current database exceeds 6500 experiments across more than 450 cell lines and tissues using a wide array of experimental techniques to study the chromatin structure, regulatory and transcriptional landscape of the H. sapiens and M. musculus genomes. All ENCODE experimental data, metadata, and associated computational analyses are submitted to the ENCODE Data Coordination Center (DCC) for validation, tracking, storage, unified processing, and distribution to community resources and the scientific community...
2017: PloS One
https://www.readbyqxmd.com/read/28399993/oregano-essential-oil-based-natural-antimicrobial-packaging-film-to-inactivate-salmonella-enterica-and-yeasts-molds-in-the-atmosphere-surrounding-cherry-tomatoes
#19
Sang-Jo Kwon, Yoonjee Chang, Jaejoon Han
This study investigated the effectiveness of a polyvinyl alcohol (PVA) film containing the natural antimicrobial oregano essential oil (OEO) as an active packaging application for decreasing the microbial growth. The film exerted an antimicrobial effect via the atmosphere surrounding the food rather than direct contact, thereby preserving the quality of cherry tomatoes. A packaging film containing microencapsulated OEO was developed. The loading content increased gradually (104.29-234.29 μg OEO/mg film) with the amount of OEO incorporated (1%, 2%, and 3%), where the PVA films containing 2% OEO had the highest loading efficiency (91...
August 2017: Food Microbiology
https://www.readbyqxmd.com/read/28399120/sotos-syndrome-associated-with-hirschsprung-s-disease-a-new-case-and-exome-sequencing-analysis
#20
Cherry Ann Sio, Kyuwhan Jung, Jeong-Hyun Kim, Hyun Sub Cheong, Eun Shin, Hyejin Jang, Miok Yoon, Huijeong Jang, Hyoung Doo Shin
BACKGROUND: Sotos syndrome (SoS) is an overgrowth disorder with various congenital anomalies and is usually accompanied by other clinical problems. However, anorectal malformations have not been documented as part of the SoS entity. Our objective is to report on a case of SoS associated with Hirschsprung's disease (HSCR) and subsequent genetic analysis. METHODS: A 2 year old male with SoS experienced constipation since infancy and ultimately showed an aganglionic segment in the histopathologic examination and was followed by exome sequencing analysis...
April 11, 2017: Pediatric Research
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