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https://www.readbyqxmd.com/read/29782890/novel-dental-adhesive-resin-with-crack-self-healing-antimicrobial-and-remineralization-properties
#1
Shichao Yue, Junling Wu, Qiang Zhang, Ke Zhang, Michael D Weir, Satoshi Imazato, Yuxing Bai, Hockin H K Xu
OBJECTIVES: Secondary caries at the tooth-restoration margins is a primary reason for restoration failure. Cracks at the margins lead to leakage which can trap bacteria, producing acids to cause caries. To date, there has been no report on developing an adhesive resin that has self-healing, antibacterial and remineralizing capabilities. The objectives of this study were to: (1) develop the first self-healing adhesive with antimicrobial and remineralizing capabilities, and (2) investigate the effects of incorporating microcapsules, dimethylaminohexadecyl methacrylate (DMAHDM) and nanoparticles of amorphous calcium phosphate (NACP) for the first time...
May 18, 2018: Journal of Dentistry
https://www.readbyqxmd.com/read/29778027/influence-of-gastrointestinal-tract-on-metabolism-of-bisphenol-a-as-determined-by-in-vitro-simulated-system
#2
Yonghua Wang, Min Rui, Yang Nie, Guanghua Lu
Oral exposure is a major route of human bisphenol A (BPA) exposure. However, influence of gastrointestinal tract on BPA metabolism is unavailable. In this study, in vitro simulator of the human intestinal microbial ecosystem (SHIME) was applied to investigate the changes in bioaccessibility and metabolism of BPA in different parts of gastrointestinal tract (stomach, small intestine and colon). Then the human hepatoma cell line HepG2 was employed to compare toxic effects of BPA itself and effluents of SHIME system on hepatic gene expression profiles...
May 7, 2018: Journal of Hazardous Materials
https://www.readbyqxmd.com/read/29777696/sterilized-bifidobacteria-suppressed-fat-accumulation-and-blood-glucose-level
#3
Keita Kikuchi, Mahmoud Ben Othman, Kazuichi Sakamoto
Probiotics such as Lactobacillus and Bifidobacterium improve the balance of intestinal microflora and have various physiological functions beneficial to human health. It is not always known whether the ingested microbial cells are viable- or killed. However, even sterilized bacterial cells are functional. Bacterial cell functions are strain-specific and their modes of action are still poorly understood. The aim of this study was to elucidate the roles of sterilized bifidobacteria in obesity and lipid metabolism...
May 16, 2018: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29776416/rapid-urease-test-rut-for-evaluation-of-urease-activity-in-oral-bacteria-in-vitro-and-in-supragingival-dental-plaque-ex-vivo
#4
Gunnar Dahlén, Haidar Hassan, Susanne Blomqvist, Anette Carlén
BACKGROUND: Urease is an enzyme produced by plaque bacteria hydrolysing urea from saliva and gingival exudate into ammonia in order to regulate the pH in the dental biofilm. The aim of this study was to assess the urease activity among oral bacterial species by using the rapid urease test (RUT) in a micro-plate format and to examine whether this test could be used for measuring the urease activity in site-specific supragingival dental plaque samples ex vivo. METHODS: The RUT test is based on 2% urea in peptone broth solution and with phenol red at pH 6...
May 18, 2018: BMC Oral Health
https://www.readbyqxmd.com/read/29774014/oral-microbiota-community-dynamics-associated-with-oral-squamous-cell-carcinoma-staging
#5
Chia-Yu Yang, Yuan-Ming Yeh, Hai-Ying Yu, Chia-Yin Chin, Chia-Wei Hsu, Hsuan Liu, Po-Jung Huang, Song-Nian Hu, Chun-Ta Liao, Kai-Ping Chang, Yu-Liang Chang
Oral squamous cell carcinoma (OSCC) is a highly aggressive cancer and the fourth leading malignancy among males in Taiwan. Some pathogenic bacteria are associated with periodontitis and oral cancer. However, the comprehensive profile of the oral microbiome during the cancer's progression from the early stage to the late stage is still unclear. We profiled the oral microbiota and identified bacteria biomarkers associated with OSCC. The microbiota of an oral rinse from 51 healthy individuals and 197 OSCC patients at different stages were investigated using 16S rRNA V3V4 amplicon sequencing, followed by bioinformatics and statistical analyses...
2018: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29771975/development-and-characterization-of-an-oral-multispecies-biofilm-implant-flow-chamber-model
#6
Nadine Kommerein, Katharina Doll, Nico S Stumpp, Meike Stiesch
Peri-implant infections are the most common cause of implant failure in modern dental implantology. These are caused by the formation of biofilms on the implant surface and consist of oral commensal and pathogenic bacteria, which harm adjacent soft and hard tissues and may ultimately lead to implant loss. In order to improve the clinical situation, there has to be a better understanding of biofilm formation on abiotic surfaces. Therefore, we successfully developed a system to cultivate an oral multispecies biofilm model in a flow chamber system, optimized for the evaluation of biofilm formation on solid materials by direct microscopic investigation...
2018: PloS One
https://www.readbyqxmd.com/read/29769762/polymicrobial-synergy-and-dysbiosis-an-overview
#7
REVIEW
Hawaabi Faqeer Mohd Shaikh, Suvarna Hanmantgouda Patil, Tanvi Shyamsundar Pangam, Khushboo Vijaysinh Rathod
The oral fissure is immensely inhabited with a number of polymicrobial colonies similar to the intestinal system. Periodontitis is a dysbiotic disease resulting from deviation in subgingival Gram-positive bacteria to Gram-negative bacteria shift from Gram-positive bacteria. The development of periodontal dysbiosis occurs over a broadened timeframe, which slowly turns the symbiotic association of host and microbe to pathogenic. This review highlights a recent paradigm of periodontitis progression has been postulated which challenges the traditional concept of periodontitis being induced by few particular periopathogens such as belonging to red complex, but by a more comprehensive dysbiotic-synergistic community...
March 2018: Journal of Indian Society of Periodontology
https://www.readbyqxmd.com/read/29769376/-lactobacillus-mucosal-vaccine-vectors-immune-responses-against-bacterial-and-viral-antigens
#8
REVIEW
Jonathan S LeCureux, Gregg A Dean
Lactic acid bacteria (LAB) have been utilized since the 1990s for therapeutic heterologous gene expression. The ability of LAB to elicit an immune response against expressed foreign antigens has led to their exploration as potential mucosal vaccine candidates. LAB vaccine vectors offer many attractive advantages: simple, noninvasive administration (usually oral or intranasal), the acceptance and stability of genetic modifications, relatively low cost, and the highest level of safety possible. Experimentation using LAB of the genus Lactobacillus has become popular in recent years due to their ability to elicit strong systemic and mucosal immune responses...
June 27, 2018: MSphere
https://www.readbyqxmd.com/read/29768968/susceptibility-of-gut-indigenous-lactic-acid-bacteria-in-balb-c-mice-to-oral-administered-lactobacillus-plantarum
#9
Takashi Kuda, Yasushi Yokota, Yutaka Haraguchi, Hajime Takahashi, Bon Kimura
Cells of Lactobacillus plantarum strains AN1 and Tennozu-SU2 exert anti-inflammatory responses in ICR mouse models of inflammatory bowel disease and protective effects against S. Typhimurium infection in BALB/c mice, respectively. To clarify the existence of L. plantarum-susceptible gut indigenous bacteria, AN1 and Tennozu-SU2 cells were administered to BALB/c mice via drinking water. Gene amplicon sequencing of 16S rRNA of caecal content revealed that the AN1 and Tennozu-SU2 cells affected the abundance of caecal indigenous lactobacilli, but the effect on the dominant Clostridiales and Bacteroidales was not clear...
May 16, 2018: International Journal of Food Sciences and Nutrition
https://www.readbyqxmd.com/read/29767123/influence-of-feeding-crimped-kernel-maize-silage-on-the-course-of-subclinical-necrotic-enteritis-in-a-broiler-disease-model
#10
Nisha Sharma, Samir Ranjitkar, Nishchal K Sharma, Ricarda M Engberg
This experiment was carried out with 375 male broilers (Ross 308) from days 1 to 28 to evaluate the influence of crimped kernel maize silage (CKMS) on the manifestation of subclinical necrotic enteritis, microbiota counts, organic acid production and relative weights of gastrointestinal segments. A necrotic enteritis disease model was applied. Birds were allocated into 3 different dietary treatments: a maize-based feed (MBF, control diet), and 2 diets supplemented with 15% (CKMS15) or 30% (CKMS30) of crimped ensiled kernel maize...
December 2017: Animal nutrition
https://www.readbyqxmd.com/read/29766819/synthesis-in-silico-molecular-docking-and-pharmacokinetic-studies-in-vitro-antimycobacterial-and-antimicrobial-studies-of-new-imidozolones-clubbed-with-thiazolidinedione
#11
Imran H Khan, Navin B Patel, Vatsal M Patel
BACKGROUND: A series of (E)-5-(4-((Z)-4-substitutedbenzylidene/2-thienylmethylene-5-oxo-2-phenyl-4,5-dihydro-1H-imidazol-1-yl) benzylidene)thiazolidine-2,4-dione were synthesized and evaluated for antimycobacterial and antimicrobial activity. All these ligands were docked against protein (InhA) Enoyl-ACP reductase of the type II fatty acid syntase (FAS-II) system, (PDB ID: 4COD). OBJECTIVE: In this report we have designed and synthesized azole scaffolds with good antitubercular activities as there is a real need to develop new candidates with less toxicity and more efficient toward pathogen...
May 15, 2018: Current Computer-aided Drug Design
https://www.readbyqxmd.com/read/29766652/mucosal-microflora-in-head-and-neck-cancer-patients
#12
A Almståhl, C Finizia, A Carlén, B Fagerberg-Mohlin, T Alstad
OBJECTIVE: To analyse the tongue and buccal microflora prospectively in head and neck cancer patients treated with radiation therapy (RT). METHODS: In 33 dentate patients, microbial samples from the tongue and buccal mucosa were collected pretreatment, during treatment, and 6 months, 1 year and 2 years post-treatment. Microorganisms associated with oral health and oral disorders were analysed using cultivation technique. Oral mucositis was scored at the appointment during treatment...
May 15, 2018: International Journal of Dental Hygiene
https://www.readbyqxmd.com/read/29766378/2-methacryloyloxyethyl-phosphorylcholine-mpc-polymer-suppresses-an-increase-of-oral-bacteria-a-single-blind-crossover-clinical-trial
#13
Natsumi Fujiwara, Hiromichi Yumoto, Koji Miyamoto, Katsuhiko Hirota, Hiromi Nakae, Saya Tanaka, Keiji Murakami, Yasusei Kudo, Kazumi Ozaki, Yoichiro Miyake
OBJECTIVES: The biocompatible 2-methacryloyloxyethyl phosphorylcholine (MPC)-polymers, which mimic a biomembrane, reduce protein adsorption and bacterial adhesion and inhibit cell attachment. The aim of this study is to clarify whether MPC-polymer can suppress the bacterial adherence in oral cavity by a crossover design. We also investigated the number of Fusobacterium nucleatum, which is the key bacterium forming dental plaque, in clinical samples. MATERIALS AND METHODS: This study was a randomized, placebo-controlled, single-blind, crossover study, with two treatment periods separated by a 2-week washout period...
May 16, 2018: Clinical Oral Investigations
https://www.readbyqxmd.com/read/29764751/needle-wash-solution-cultures-following-ebus-tbna-with-or-without-endobronchial-intubation
#14
Daisuke Minami, Nagio Takigawa, Masahide Oki, Hideo Saka, Takuo Shibayama, Katsuyuki Kiura
BACKGROUND: Endobronchial ultrasound-guided transbronchial needle aspiration (EBUS-TBNA) is a minimally invasive procedure with a high diagnostic yield in lesions adjacent to the airways. However, complications associated with EBUS-TBNA, such as mediastinitis, have recently been reported. Oral bacteria contamination in punctured lymph nodes can cause severe infections. In the current study, we investigated whether endobronchial intubation using EBUS-TBNA can prevent oral bacterial contamination of punctured lymph nodes...
May 10, 2018: Respiratory Investigation
https://www.readbyqxmd.com/read/29764197/re-thinking-the-chicken-campylobacter-jejuni-interaction-a-review
#15
Wageha A Awad, Claudia Hess, Michael Hess
Chickens are recognized as an imperative source of thermophilic Campylobacter spp., carrying this microorganism in high numbers in their intestinal tract. For long time, C. jejuni has been considered as a commensal microorganism which colonizes its primary host rather than infecting it, in the absence of any obvious clinical signs. However, recent studies question this and argue for a deeper understanding of the host-bacteria interaction. Following oral uptake it was demonstrated that C. jejuni interacts intimately with the gut epithelium and influences cellular functions of the host, with consequences on nutrient absorption...
May 15, 2018: Avian Pathology: Journal of the W.V.P.A
https://www.readbyqxmd.com/read/29761905/stress-related-salivary-proteins-affect-the-production-of-volatile-sulfur-compounds-by-oral-bacteria
#16
Patricia Oliveira de Lima, Bruno Dias Nani, Barbara Almeida, Fernanda Klein Marcondes, Francisco Carlos Groppo, Antonio Bento Alves de Moraes, Michelle Franz-Montan, Karina Cogo-Müller
OBJECTIVE: To determine whether stress-related substances and sex hormones influence the growth and in vitro production of volatile sulfur compounds (VSCs) by Solobacterium moorei and Fusobacterium nucleatum. MATERIALS AND METHODS: Bacteria growth and VSCs production were evaluated in the presence of alpha-amylase, beta-defensin-2, mucin, estradiol, and progesterone. Growth was evaluated by colony counting, and the production of the VSCs hydrogen sulfide (H2 S) and methyl mercaptan (CH3 SH) was measured using the Oral Chroma™ instrument...
May 15, 2018: Oral Diseases
https://www.readbyqxmd.com/read/29760169/dysbiosis-of-maternal-and-neonatal-microbiota-associated-with-gestational-diabetes-mellitus
#17
Jinfeng Wang, Jiayong Zheng, Wenyu Shi, Nan Du, Xiaomin Xu, Yanming Zhang, Peifeng Ji, Fengyi Zhang, Zhen Jia, Yeping Wang, Zhi Zheng, Hongping Zhang, Fangqing Zhao
OBJECTIVE: The initial colonisation of the human microbiota and the impact of maternal health on neonatal microbiota at birth remain largely unknown. The aim of our study is to investigate the possible dysbiosis of maternal and neonatal microbiota associated with gestational diabetes mellitus (GDM) and to estimate the potential risks of the microbial shift to neonates. DESIGN: Pregnant women and neonates suffering from GDM were enrolled and 581 maternal (oral, intestinal and vaginal) and 248 neonatal (oral, pharyngeal, meconium and amniotic fluid) samples were collected...
May 14, 2018: Gut
https://www.readbyqxmd.com/read/29756986/-dietetic-treatment-with-fructose-in-a-5-year-old-girl-with-recurrent-d-lactic-acidosis
#18
Lourdes Travieso Suárez, Pilar Quijada Fraile, Consuelo Pedrón Giner
D-lactic acidosis is an infrequent complication, mainly reported in patients with short bowel syndrome. It is characterized by recurrent episodes of encephalopathy with elevated serum D-lactic acid, usually associating metabolic acidosis. The presence of D-lactate-producing bacteria is necessary for the development of this complication. Other factors, such as the ingestion of large amounts of carbohydrates or reduced intestinal motility, contribute to D-lactic acidosis. We report a case of recurrent D-lactic acidosis in a 5-year-old girl with short bowel syndrome, due to a midgut volvulus...
March 1, 2018: Nutrición Hospitalaria: Organo Oficial de la Sociedad Española de Nutrición Parenteral y Enteral
https://www.readbyqxmd.com/read/29756653/the-effect-of-photodynamic-therapy-on-pathogenic-bacteria-around-peri-implant-sulcus-and-in-the-cavity-between-abutment-and-implant-after-healing-phase-a-prospective-clinical-study
#19
Lin-Yi Zhou, Jun-Yu Shi, Yu Zhu, Shu-Jiao Qian, Hong-Chang Lai, Ying-Xin Gu
OBJECTIVES: To compare levels of pathogens from peri-implant sulcus versus abutment screw cavities after photodynamic therapy. MATERIAL AND METHODS: Twenty patients were included. Photodynamic therapy (PDT) was applied both in sulcus and cavities after sampling following suprastructures loading, and repeated after 2 weeks. Two samples each containing four paper points were collected for each implant at baseline, 2 weeks, 3 months: (i) peri-implant sulcus and (ii) abutment screw cavities...
May 14, 2018: Lasers in Surgery and Medicine
https://www.readbyqxmd.com/read/29756429/bacterial-osteomyelitis-microbiological-clinical-therapeutic-and-evolutive-characteristics-of-344-episodes
#20
E García Del Pozo, J Collazos, J A Cartón, D Camporro, V Asensi
OBJECTIVE: Osteomyelitis is a difficult-to-cure infection, with high relapse rate despite adequate therapy. Large published osteomyelitis series in adults are rare. METHODS: A total of 344 adult osteomyelitis patients were studied and followed > 12 months after hospital discharge. Demographic, microbiological, clinical, therapeutic and outcome data were analyzed. RESULTS: Mean age was 52.5 ± 18.3 years and 233 (67.7%) were male. Main osteomyelitis types were post-surgical (31...
May 11, 2018: Revista Española de Quimioterapia: Publicación Oficial de la Sociedad Española de Quimioterapia
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