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https://www.readbyqxmd.com/read/28736550/probiotic-enterococcus-mundtii-isolate-protects-the-model-insect-tribolium-castaneum-against-bacillus-thuringiensis
#1
Thorben Grau, Andreas Vilcinskas, Gerrit Joop
Enterococcus mundtii strains isolated from the larval feces of the Mediterranean flour moth Ephestia kuehniella show antimicrobial activity against a broad spectrum of Gram-positive and Gram-negative bacteria. The in vitro probiotic characterization of one isolate revealed a high auto-aggregation score, a hydrophilic cell surface, tolerance for low pH, no hemolytic activity, and susceptibility to all tested antibiotics. We used the red flour beetle Tribolium castaneum, an established model organism, for the in vivo characterization of one probiotic E...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28736072/antibacterial-activities-of-natural-lichen-compounds-against-streptococcus-gordonii-and-porphyromonas-gingivalis
#2
Alaa Sweidan, Marylene Chollet-Krugler, Aurelie Sauvager, Pierre van de Weghe, Ali Chokr, Martine Bonnaure-Mallet, Sophie Tomasi, Latifa Bousarghin
The oral bacteria not only infect the mouth and reside there, but also travel through the blood and reach distant body organs. If left untreated, the dental biofilm that can cause destructive inflammation in the oral cavity may result in serious medical complications. In dental biofilm, Streptococcus gordonii, a primary oral colonizer, constitutes the platform on which late pathogenic colonizers like Porphyromonas gingivalis, the causative agent of periodontal diseases, will bind. The aim of this study was to determine the antibacterial activity of eleven natural lichen compounds belonging to different chemical families and spanning from linear into cyclic and aromatic structures to uncover new antibiotics which can fight against the oral bacteria...
July 20, 2017: Fitoterapia
https://www.readbyqxmd.com/read/28736041/construction-of-multilayer-alginate-hydrogel-beads-for-oral-delivery-of-probiotics-cells
#3
Yang Li, Chao Feng, Jing Li, Yuzhi Mu, Ya Liu, Ming Kong, Xiaojie Cheng, Xiguang Chen
Multilayer alginate hydrogel beads (MAHBs), which were prepared by emulsion method, had been fabricated via the ionic crosslinking between calcium ion (Ca(2+)) and carboxylic group of alginate and utilized as encapsulating material for oral delivery of a model probiotic bacteria Bifidobacterium breve. Optical and fluorescence microscopy obviously showed the microorganism cells were encapsulated in the core beads. The viability of B. breve cells encapsulated in MAHBs was significantly enhanced compared with ordinary free cultured ones, the top CFU per mL were (3...
July 20, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28736026/yogurt-feeding-induced-the-prolongation-of-fully-major-histocompatibility-complex-mismatched-murine-cardiac-graft-survival-by-induction-of-cd4-foxp3-cells
#4
M Uchiyama, E Yin, T Yanagisawa, X Jin, M Hara, S Matsuyama, T Imazuru, K Uchida, M Kawamura, M Niimi
Yogurt is a nutrient-rich food and the beneficial effects of yogurt on both health and immunomodulatory effects are well documented. In this pilot study, we investigated the effects of commercially produced yogurt R-1 on alloimmune responses in a murine cardiac transplantation model. The R-1 is produced by Meiji Co., Ltd., and contains live and active lactic acid bacteria (lactobacillus bulgaricus OLL1073R-1) mainly. CBA (H2(k)) mice underwent transplantation of a C57BL/6 (H2(b); B6) heart and received oral administration of 1 mL, 0...
July 2017: Transplantation Proceedings
https://www.readbyqxmd.com/read/28735651/the-effect-of-artesunate-on-short-term-memory-in-lyme-borreliosis
#5
B K Puri, J S Hakkarainen-Smith, J A Monro
Lyme borreliosis is associated with memory deficits. While this may be related to cerebral infection by Borrelia bacteria, it may also be caused by concomitant co-infection by Babesia protozoa. The anti-malarial artemisinin-derivative artesunate has been shown to be effective against a number of Babesia species and to have efficacy against human cerebral malaria. We hypothesised that concomitant administration of artesunate in Lyme borreliosis patients would help alleviate the severity of self-reported short-term memory impairment...
August 2017: Medical Hypotheses
https://www.readbyqxmd.com/read/28733908/assessment-of-cultivable-oral-bacterial-flora-from-important-venomous-snakes-of-india-and-their-antibiotic-susceptibilities
#6
Innus K Shaikh, Prashant P Dixit, Balasaheb S Pawade, Mugdha Potnis-Lele, Babasaheb P Kurhe
Snakebite is a common, frequently devastating, occupational, socio-economic hazard, and it has a great impact on the rural population of India. Snakebite is a major cause of the human morbidity and mortality since ancient times, as it not only affects the victim by systemic envenomation but also by wound infections originating from deadly pathogenic microorganisms from the oral cavity of the offending snake. The pathogens from the oral cavity of the snake tend to initiate an infection, resulting in gas gangrene, soft tissue necrosis, and permanent physical disabilities...
July 21, 2017: Current Microbiology
https://www.readbyqxmd.com/read/28733891/consequences-of-bisphenol-a-perinatal-exposure-on-immune-responses-and-gut-barrier-function-in-mice
#7
Yann Malaisé, Sandrine Ménard, Christel Cartier, Corinne Lencina, Caroline Sommer, Eric Gaultier, Eric Houdeau, Laurence Guzylack-Piriou
The potent immunomodulatory effect of the endocrine disruptor bisphenol A during development and consequences during life span are of increasing concern. Particular interests have been raised from animal studies regarding the risk of developing food intolerance and infection. We aimed to identify immune disorders in mice triggered by perinatal exposure to bisphenol A. Gravid mice were orally exposed to bisphenol (50 μg/kg body weight/day) from day 15 of pregnancy until weaning. Gut barrier function, local and systemic immunity were assessed in adult female offspring...
July 21, 2017: Archives of Toxicology
https://www.readbyqxmd.com/read/28733289/an-enterotoxin-like-binary-protein-from-pseudomonas-protegens-with-potent-nematicidal-activity
#8
Jun-Zhi Wei, Daniel L Siehl, Zhenglin Hou, Barbara Rosen, Jarred Oral, Christopher G Taylor, Gusui Wu
Soil microbes are a major food source for free-living soil nematodes. It is known that certain soil bacteria have evolved systems to combat predation. We identified the nematode-antagonistic Pseudomonas protegens strain 15G2 from screening of microbes. Through protein purification we identified a binary protein, designated as Pp-ANP, that is responsible for the nematicidal activity. This binary protein inhibits Caenorhabditis elegans growth and development by arresting larvae at L1 stage and killing older staged worms...
July 21, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/28730918/clinical-and-economic-implications-of-urinary-tract-infections
#9
Samantha N Steiger, Rachel R Comito, David P Nicolau
Urinary tract infections represent one of the most frequent reasons for hospitalization. As a result of their prevalence from community-based origins as well as those which develop in hospital setting, this constellation of infections represents a tremendous burden to the global healthcare system. Areas Covered: Over the last several decades the management of these infections has become more complicated due to the underlying comorbid conditions of the patients as well as escalating antimicrobial resistance to many of the most frequently used oral and parenteral agents...
July 21, 2017: Expert Review of Pharmacoeconomics & Outcomes Research
https://www.readbyqxmd.com/read/28730144/molecular-characterization-of-the-human-stomach-microbiota-in-gastric-cancer-patients
#10
Guoqin Yu, Javier Torres, Nan Hu, Rafael Medrano-Guzman, Roberto Herrera-Goepfert, Michael S Humphrys, Lemin Wang, Chaoyu Wang, Ti Ding, Jacques Ravel, Philip R Taylor, Christian C Abnet, Alisa M Goldstein
Helicobacter pylori (Hp) is the primary cause of gastric cancer but we know little of its relative abundance and other microbes in the stomach, especially at the time of gastric cancer diagnosis. Here we characterized the taxonomic and derived functional profiles of gastric microbiota in two different sets of gastric cancer patients, and compared them with microbial profiles in other body sites. Paired non-malignant and tumor tissues were sampled from 160 gastric cancer patients with 80 from China and 80 from Mexico...
2017: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/28729722/structure-based-discovery-of-small-molecule-inhibitors-of-cariogenic-virulence
#11
Qiong Zhang, Bhavitavya Nijampatnam, Zhang Hua, Thao Nguyen, Jing Zou, Xia Cai, Suzanne M Michalek, Sadanandan E Velu, Hui Wu
Streptococcus mutans employs a key virulence factor, three glucosyltransferase (GtfBCD) enzymes to establish cariogenic biofilms. Therefore, the inhibition of GtfBCD would provide anti-virulence therapeutics. Here a small molecule library of 500,000 small molecule compounds was screened in silico against the available crystal structure of the GtfC catalytic domain. Based on the predicted binding affinities and drug-like properties, small molecules were selected and evaluated for their ability to reduce S. mutans biofilms, as well as inhibit the activity of Gtfs...
July 20, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28728145/extracellular-dna-contributes-to-dental-biofilm-stability
#12
Sebastian Schlafer, Rikke L Meyer, Irene Dige, Viduthalai R Regina
Extracellular DNA (eDNA) is a major matrix component of many bacterial biofilms. While the presence of eDNA and its role in biofilm stability have been demonstrated for several laboratory biofilms of oral bacteria, there is no data available on the presence and function of eDNA in in vivo grown dental biofilms. This study aimed to determine whether eDNA was part of the matrix in biofilms grown in situ in the absence of sucrose and whether treatment with DNase dispersed biofilms grown for 2.5, 5, 7.5, 16.5, or 24 h...
July 21, 2017: Caries Research
https://www.readbyqxmd.com/read/28727999/drug-ion-co-delivery-multi-functional-nanocarrier-to-regenerate-infected-tissue-defect
#13
Jung-Hwan Lee, Ahmed El-Fiqi, Nandin Mandakhbayar, Hae-Hyoung Lee, Hae-Won Kim
Regeneration of infected tissues is a globally challenging issue in medicine and dentistry. Common clinical therapies involving a complete removal of infected areas together with a treatment of antimicrobial drugs are often suboptimal. Biomaterials with anti-bacterial and pro-regenerative potential can offer a solution to this. Here we design a novel nanocarrier based on a mesoporous silicate-calcium glass by doping with Ag ions and simultaneously loading antimicrobial drugs onto mesopores. The nanocarriers could controllably release multiple ions (silver, calcium, and silicate) and drugs (tetracycline or chlorohexidine) to levels therapeutically relevant, and effectively internalize to human dental stem cells (∼90%) with excellent viability, ultimately stimulating odontogenic differentiation...
July 12, 2017: Biomaterials
https://www.readbyqxmd.com/read/28725299/role-of-streptococcus-mutans-two-component-systems-in-antimicrobial-peptide-resistance-in-the-oral-cavity
#14
REVIEW
Miki Kawada-Matsuo, Hitoshi Komatsuzawa
Approximately 100 trillion microorganisms exist in the oral cavity. For the commensal bacteria of the oral cavity, it is important to adapt to environmental stimuli, including human- or bacteria-derived antimicrobial agents. Recently, bacterial-specific signal transduction regulatory systems, called two-component systems (TCSs), which appear to be focused on sensing and adapting to the environment, were discovered. Streptococcus mutans is an oral commensal bacteria and is also known as a cariogenic bacteria...
August 2017: Japanese Dental Science Review
https://www.readbyqxmd.com/read/28724921/saliva-and-tooth-biofilm-bacterial-microbiota-in-adolescents-in-a-low-caries-community
#15
Linda Eriksson, Pernilla Lif Holgerson, Ingegerd Johansson
The oral cavity harbours a complex microbiome that is linked to dental diseases and serves as a route to other parts of the body. Here, the aims were to characterize the oral microbiota by deep sequencing in a low-caries population with regular dental care since childhood and search for association with caries prevalence and incidence. Saliva and tooth biofilm from 17-year-olds and mock bacteria communities were analysed using 16S rDNA Illumina MiSeq (v3-v4) and PacBio SMRT (v1-v8) sequencing including validity and reliability estimates...
July 19, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28724342/periodontal-disease-and-intra-amniotic-complications-in-women-with-preterm-prelabor-rupture-of-membranes
#16
Vladimira Radochova, Ivana Kacerovska Musilova, Martin Stepan, Peter Vescicik, Radovan Slezak, Bo Jacobsson, Marian Kacerovsky
OBJECTIVE: Periodontal disease is frequently suggested as a possible causal factor for preterm delivery. The link between periodontal disease and preterm delivery is a possible translocation of periopathogenic bacteria to the placenta and amniotic fluid as well as a systemic response to this chronic inflammatory disease. However, there is a lack of information on whether there is an association between clinical periodontal status in women with preterm prelabor rupture of membranes (PPROM) and the presence of microbial invasion of the amniotic cavity (MIAC) and intra-amniotic inflammation (IAI)...
July 20, 2017: Journal of Maternal-fetal & Neonatal Medicine
https://www.readbyqxmd.com/read/28724047/correlation-between-virulence-markers-of-helicobacter-pylori-in-the-oral-cavity-and-gastric-biopsies
#17
Myriam Lucrecia Medina, Marcelo Gabriel Medina, Luis Antonio Merino
BACKGROUND: The clinical outcome of Helicobacter pylori infection has been associated with virulence factors. The presence of these factors is useful as molecular markers in the identification of the high risk for developing severe gastric pathologies. OBJECTIVE: To correlate the presence of virulence markers cagA and bab2A of H. pylori in oral and gastric biopsy samples. METHODS: An observational, prospective, descriptive, and cross-sectional study was carried out between September 2011 and September 2012...
July 13, 2017: Arquivos de Gastroenterologia
https://www.readbyqxmd.com/read/28721820/epigenetic-regulations-in-the-pathogenesis-of-periodontitis
#18
Yu Luo, Xian Peng, Dingyu Duan, Chengcheng Liu, Xin Xu, Xuedong Zhou
Periodontitis is a multifactorial infectious disease that affects a large population worldwide. It involves progressive alveolar bone loss, and if left untreated, can lead to the loosening and subsequent loss of teeth. Oral microorganisms and susceptible host have been proposed to be the prerequisite for the development of periodontitis. Aberrant host immune response is the key factor that leads to the destruction of periodontal tissues. Recently, the involvement of epigenetic modifications in the development of periodontitis has attracted increasing attentions...
July 18, 2017: Current Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/28721668/quantitative-analysis-of-s-mutans-lactobacillus-and-bifidobacterium-found-in-initial-and-mature-plaques-in-thai-children-with-early-childhood-caries
#19
K Mitrakul, S Chanvitan, A Jeamset, K Vongsawan
AIMS: To quantify Streptococcus mutans, lactobacillus and bifidobacterium in initial and mature plaque collected from children with severe early childhood caries (S-ECC) and caries-free (CF) groups and to analyse the association between these bacteria and caries-related factors in each group. STUDY DESIGN: A collection of 120 initial and overnight supra-gingival plaques were collected from Thai children aged 2-5 years-old (S-ECC = 60, CF = 60). Plaque, gingival indices and decayed, missing, filled tooth (dmft) scores were recorded...
July 18, 2017: European Archives of Paediatric Dentistry: Official Journal of the European Academy of Paediatric Dentistry
https://www.readbyqxmd.com/read/28721297/potential-application-of-bacillus-subtilis-spb1-lipopeptides-in-toothpaste-formulation
#20
Mouna Bouassida, Nada Fourati, Fatma Krichen, Raida Zouari, Semia Ellouz-Chaabouni, Dhouha Ghribi
Toothpaste is a gel dentifrice used with a toothbrush as an accessory to clean, keep and promote oral hygiene. The literature review suggests that there are many different formulations of toothpastes and that each of their individual components present specific functions. The concentration of the toothpaste ingredients must be appropriately chosen taking into account the purposes of the toothpaste. Biosurfactants are considered as suitable molecules for application in many formulations such as in toothpaste one...
July 2017: Journal of Advanced Research
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