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https://www.readbyqxmd.com/read/29235434/ige-antibodies-to-alpha-gal-in-foresters-and-forest-workers-from-la-rioja-north-of-spain
#1
M Venturini, T Lobera, A Sebastián, A Portillo, J A Oteo
BACKGROUND AND OBJECTIVE: To investigate the prevalence and associated factors to the presence of alpha-gal-specific IgE in a risk group of foresters and forest workers from La Rioja and in a control group. METHODS: A total of 169 workers and 100 individuals who did not recall tick-bites were selected. A questionnaire including demographic data and number of tick bites per year was administered by a physician. Alpha-gal sIgE was assessed with serum samples that had been taken in 2010 using ImmunoCAP®...
December 13, 2017: Journal of Investigational Allergology & Clinical Immunology
https://www.readbyqxmd.com/read/29161766/identification-of-alpha-gal-sensitivity-in-patients-with-a-diagnosis-of-idiopathic-anaphylaxis
#2
Melody C Carter, Karina N Ruiz-Esteves, Lisa Workman, Philip Lieberman, Thomas A E Platts-Mills, Dean D Metcalfe
IgE antibodies (Ab) specific to galactose-α-1,3-galactose (alpha-gal) are responsible for a delayed form of anaphylaxis that occurs 3 to 6 hours after red meat ingestion. In a unique prospective study of seventy participants referred with a diagnosis of idiopathic anaphylaxis (IA), six (9%) were found to have IgE to alpha-gal. Upon institution of a diet free of red meat, all patients had no further episodes of anaphylaxis. Two of these individuals had indolent systemic mastocytosis (ISM). Those with ISM had more severe clinical reactions but lower specific IgE to alpha-gal and higher serum tryptase levels, reflective of the mast cell burden...
November 21, 2017: Allergy
https://www.readbyqxmd.com/read/29074067/a-tick-acquired-red-meat-allergy-a-case-series
#3
Joe Kevin Khoury, Neil Christian Khoury, Deborah Schaefer, Anup Chitnis, Getaw Worku Hassen
Allergic reaction is a common clinical picture in the Emergency Department (ED). Most allergic reactions are from food or drugs. A detailed history is an integral aspect of determining the causative agent of an allergy. Galactose-alpha-1,3-galactose (alpha-gal) allergy is a tick-acquired red meat allergy that causes delayed-onset allergic reaction or anaphylaxis due to molecular mimicry. Alpha-gal allergy may not be widely known as a cause of allergic reactions. Lack of universal awareness of this phenomenon in the ED and Urgent Care setting could lead to misdiagnosis, or delayed diagnosis...
October 16, 2017: American Journal of Emergency Medicine
https://www.readbyqxmd.com/read/29057501/different-approaches-to-heart-valve-decellularization-a-comprehensive-overview-of-the-past-30%C3%A2-years
#4
REVIEW
Filippo Naso, Alessandro Gandaglia
Xenogeneic decellularized heart valve scaffolds have the potential to overcome the limitations of existing bioprosthetic heart valves that have limited duration due to calcification and tissue degeneration phenomena. This article presents a review of 30 years of decellularization approaches adopted in cardiovascular tissue engineering, with a focus on the use, either individually or in combination, of different detergents. The safety and efficacy of cell-removal procedures are specifically reported and discussed, as well as the structure and biomechanics of the treated extracellular matrix (ECM)...
October 22, 2017: Xenotransplantation
https://www.readbyqxmd.com/read/29053434/alpha-gal-inactivated-heart-valve-bioprostheses-exhibit-an-anti-calcification-propensity-similar-to-knockout-tissues
#5
Filippo Naso, Ugo Stefanelli, Edward Buratto, Giovanna Lazzari, Andrea Perota, Cesare Galli, Alessandro Gandaglia
BACKGROUND: Glutaraldehyde (GLA) has been used to crosslink bioprosthetic heart valve (BHVs) tissues to enhance their stability, besides ensuring a satisfactory degree of immunological tolerance. Unfortunately, GLA fixation does not guarantee a complete tissue biocompatibility of BHVs in currently used devices. The interaction between preformed human anti-alpha-Gal antibody and alpha-Gal antigens promotes the calcification of GLA-treated alpha-Gal-positive tissue. Recently, an alarming correlation between the presence of the alpha-Gal epitope and a premature BHVs degeneracy was reported...
October 2017: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/28972138/photorhabdus-luminescens-lectin-a-plla-a-new-probe-for-detecting-%C3%AE-galactoside-terminating-glycoconjugates
#6
Ghamdan Beshr, Asfandyar Sikandar, Eva-Maria Jemiller, Nikolai Klymiuk, Dirk Hauck, Stefanie Wagner, Eckhard Wolf, Jesko Koehnke, Alexander Titz
Lectins play important roles in infections by pathogenic bacteria, for example, in host colonization, persistence and biofilm formation. The Gram-negative entomopathogenic bacterium Photorhabdus luminescens symbiotically lives in insect-infecting Heterorhabditis nematodes and kills the insect host upon invasion by the nematode. The P. luminescens genome harbors the gene plu2096 coding for a novel lectin that we named PllA. We analyzed the binding properties of purified PllA with a glycan array and a binding assay in solution...
September 28, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28921428/production-of-recombinant-factor-viii-in-human-cell-lines
#7
Daianne Maciely Carvalho Fantacini, Virgínia Picanço-Castro
Human cell lines can produce recombinant proteins much more similar to their natural counterpart, compared to other mammalian cell lines, reducing potential immunogenic reactions. Recombinant proteins produced in nonhuman cells can have in its structure glycan epitopes, such as Galα1,3-Gal (alpha-Gal) and N-glycolylneuraminic acid (Neu5Gc) residues, that are antigenic to humans and can potentially affect the efficacy of the recombinant product. Therefore, the production of recombinant factor VIII (rFVIII) in human cell lines is a new approach to avoid nonhuman glycosylation...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28815010/development-and-characterisation-of-a-low-concentration-sodium-dodecyl-sulphate-decellularised-porcine-dermis
#8
Jack A Helliwell, Daniel S Thomas, Vaia Papathanasiou, Shervanthi Homer-Vanniasinkam, Amisha Desai, Louise M Jennings, Paul Rooney, John N Kearney, Eileen Ingham
The aim of this study was to adapt a proprietary decellularisation process for human dermis for use with porcine skin. Porcine skin was subject to: sodium chloride (1 M) to detach the epidermis, trypsin paste to remove hair follicles, peracetic acid (0.1% v/v) disinfection, washed in hypotonic buffer and 0.1% (w/v) sodium dodecyl sulphate in the presence of proteinase inhibitors followed by nuclease treatment. Cellular porcine skin, decellularised porcine and human dermis were compared using histology, immunohistochemistry, GSL-1 lectin (alpha-gal epitope) staining, biochemical assays, uniaxial tensile and in vitro cytotoxicity tests...
January 2017: Journal of Tissue Engineering
https://www.readbyqxmd.com/read/28752723/red-meat-allergy-induced-by-tick-bites-a-norwegian-case-report
#9
G Arslan Lied
Food allergies, especially delayed hypersensitivity reactions, are often challenging for both patients and clinicians. Here, we report the case of a 64-year-old man who had allergic reactions six hours after eating a meal containing red meat. He reported that he had several tick bites in months before the reaction. High serum specific IgE levels of alpha-gal confirmed the diagnosis of alpha-gal allergic reaction with delayed onset after red meat ingestion caused by tick bite.
July 2017: European Annals of Allergy and Clinical Immunology
https://www.readbyqxmd.com/read/28670695/meat-allergy-associated-with-%C3%AE-gal-closing-diagnostic-gaps-by-anti-%C3%AE-gal-ige-immune-profiling
#10
U Jappe, S Minge, B Kreft, A Ludwig, B Przybilla, A Walker, R Varga, P Seidel, T Biedermann, W Anemüller, A Kromminga, F Ruëff, H Merk, N Wagner, R Treudler, M Worm, I Waldmann, J Saloga, W M Becker, T Goldmann, T A Platts-Mills, A Homann
BACKGROUND: Glycoproteins and glycolipids of some mammalian species contain the disaccharide galactosyl-α-(1,3)-galactose (α-Gal). It is known that α-Gal is immunogenic in humans and causes glycan-specific IgG and also IgE responses with clinical relevance. α-Gal is part of the IgE-reactive monoclonal therapeutic antibody cetuximab and is associated with delayed anaphylaxis to red meat. In this study, different alpha-Gal-containing analytes are examined in singleplex and multiplex assays to resolve individual sensitization patterns with IgE against α-Gal...
July 3, 2017: Allergy
https://www.readbyqxmd.com/read/28497612/accurate-assessment-of-alpha-gal-syndrome-using-cetuximab-and-bovine-thyroglobulin-specific-ige
#11
Da Woon Sim, Jong Sun Lee, Kyung Hee Park, Kyoung Yong Jeong, Young-Min Ye, Jae-Hyun Lee, Jung-Won Park
SCOPE: IgE against galactose-α-1,3-galactose (α-Gal) causes alpha-gal syndrome. Bovine thyroglobulin (BTG) and cetuximab share this epitope. We aimed to determine the utility of specific IgE (sIgE) against cetuximab as compared to BTG for diagnosing alpha-gal syndrome. METHODS AND RESULTS: Twelve patients with alpha-gal syndrome, 11 patients with immediate beef or pork allergy, 18 asymptomatic individuals with meat sensitization, and 10 non-atopic subjects were enrolled...
May 12, 2017: Molecular Nutrition & Food Research
https://www.readbyqxmd.com/read/28472163/covalent-decoration-of-adenovirus-vector-capsids-with-the-carbohydrate-epitope-%C3%AE-gal-does-not-improve-vector-immunogenicity-but-allows-to-study-the-in-vivo-fate-of-adenovirus-immunocomplexes
#12
Ramona F Kratzer, Sigrid Espenlaub, Andrea Hoffmeister, Matthias W Kron, Florian Kreppel
Adenovirus-based vectors are promising tools for genetic vaccination. However, several obstacles have to be overcome prior to a routine clinical application of adenovirus-based vectors as efficacious vectored vaccines. The linear trisaccharide epitope αGal (alpha-Gal) with the carbohydrate sequence galactose-α-1,3-galactosyl-β-1,4-N-acetylglucosamine has been described as a potent adjuvant for recombinant or attenuated vaccines. Humans and α-1,3-galactosyltransferase knockout mice do not express this epitope...
2017: PloS One
https://www.readbyqxmd.com/read/28447914/diagnosis-of-life-threatening-alpha-gal-food-allergy-appears-to-be-patient-driven
#13
Mary Grace Flaherty, Samantha Jan Kaplan, Maya R Jerath
OBJECTIVE: Patients exhibiting life-threatening symptoms associated with the alpha-gal food allergy (delayed urticaria or anaphylaxis due to mammalian meat) are frequently undiagnosed, causing unnecessary emergency department (ED) and health care visits, and extensive pain and suffering. This study aimed to determine the path to diagnosis experienced by alpha-gal patients. METHODS: Semistructured interviews were conducted from March to June 2016 with a chronological systematic sample of approximately 10% of patients diagnosed with alpha-gal and treated by the University of North Carolina Allergy and Immunology Clinic (n = 28)...
April 1, 2017: Journal of Primary Care & Community Health
https://www.readbyqxmd.com/read/28380688/fabrication-and-characterization-of-a-decellularized-bovine-tendon-sheet-for-tendon-reconstruction
#14
Liang-Ju Ning, Yan-Lin Jiang, Cheng-Hao Zhang, Yi Zhang, Jie-Liang Yang, Jing Cui, Yan-Jing Zhang, Xuan Yao, Jing-Cong Luo, Ting-Wu Qin
Obtaining a performing decellularized tendon scaffold with proper dimensions and adequate availability is highly desirable. However, the combined study of complete decellularization and detailed characterization of native tendon extracellular matrix (ECM) from large animals is still lacking. In the present study, we developed a new decellularization protocol, including physical methods and enzymatic solutions for processing bovine Achilles tendons, and produced a decellularized bovine tendon sheet (DBTS) scaffold for tendon reconstruction...
April 5, 2017: Journal of Biomedical Materials Research. Part A
https://www.readbyqxmd.com/read/28360401/mutational-analysis-of-the-gla-gene-in-mexican-families-with-fabry-disease
#15
Bianca Ethel Gutiérrez-Amavizca, Andreas Gal, Rocío Ortíz-Orozco, Ulrich Orth, Ernesto Prado Montes De Oca, Jaime Paul Gutiérrez-Amavizca, Luis E Figuera
Fabry disease (FD) is a lysosomal storage disorder, which develops due to a deficiency in the hydrolytic enzyme, α-galactosidase A (α-Gal A). Alpha-Gal A hydrolyzes glycosphingolipid globotriaosylceramide (Gb3), and an α-Gal A deficiency leads to Gb3 accumulation in tissues and cells in the body. This pathology is likely to involve multiple systems, but it is generally considered to affect primarily vascular endothelium. In this study, we investigated mutations in the GLA gene, which encodes α-Gal A, in Mexican families with FD...
March 2017: Journal of Genetics
https://www.readbyqxmd.com/read/28294930/-the-alpha-gal-syndrome-an-allergic-reaction-to-mammalian-meat-secondary-to-a-tick-bite
#16
M A Berends, J N G Oude Elberink
Here we present the case of a 68-year-old patient with alpha-gal syndrome. This is a delayed-onset allergic reaction, characteristically occurring 2-6 hours after ingestion of mammalian meat products. The reaction occurs because the patient has developed IgE antibodies to a mammalian oligosaccharide epitope, galactose-α-1,3-galactose (alpha-gal); tick bites induce this IgE antibody response. Presentation varies from chronic urticaria to life-threatening anaphylaxis. The alpha-gal syndrome is usually self-limiting as long as there are no new tick bites...
2017: Nederlands Tijdschrift Voor Geneeskunde
https://www.readbyqxmd.com/read/28273338/prevalence-of-type-i-sensitization-to-alpha-gal-in-forest-service-employees-and-hunters
#17
J Fischer, E Lupberger, J Hebsaker, G Blumenstock, E Aichinger, A S Yazdi, D Reick, R Oehme, T Biedermann
BACKGROUND: The production of IgE molecules specific to the carbohydrate galactose-α-1,3-galactose (alpha-gal) is known to induce delayed anaphylaxis against mammalian meat. Tick bites constitute the primary sensitization source, as ticks transfer alpha-gal in their saliva to a host during a bite. The reported prevalence of alpha-gal-specific IgE (alpha-gal-sIgE) positivity varies between different populations from diverse geographic regions. OBJECTIVE: To investigate the prevalence of alpha-gal-sIgE positivity in a population of forest service employees who are highly exposed to ticks in comparison with a residential population and a historic sample...
October 2017: Allergy
https://www.readbyqxmd.com/read/28238108/enhanced-vascular-biocompatibility-of-decellularized-xeno-allogeneic-matrices-in-a-rodent-model
#18
M van Steenberghe, T Schubert, Y Guiot, C Bouzin, X Bollen, P Gianello
Glutaraldehyde preservation is the gold standard for cardiovascular biological prosthesis. However, secondary calcifications and the absence of tissue growth remain major limitations. Our study assessed in vitro and in vivo the biocompatibility of human (fascia lata, pericardium) and porcine tissues (pericardium, peritoneum) treated with a physicochemical procedure for decellularization and non-conventional pathogens inactivation. Biopsies were performed before and after treatment to assess decellularization (HE/Dapi staining/DNA quantification/MHC I/alpha gal immunostaining) and mechanical integrity...
June 2017: Cell and Tissue Banking
https://www.readbyqxmd.com/read/28224342/galactose-%C3%AE-1-3-galactose-atypical-food-allergen-or-model-ige-hypersensitivity
#19
REVIEW
Jeffrey M Wilson, Alexander J Schuyler, Nikhila Schroeder, Thomas A E Platts-Mills
PURPOSE OF REVIEW: Galactose-α-1,3-galactose (α-gal) is a carbohydrate allergen with several unique characteristics. In this article, we discuss some recent advances in our understanding of the 'alpha-gal syndrome,' highlight data supporting the role of ticks in pathogenesis, and speculate on immune mechanisms that lead to sensitization. RECENT FINDINGS: First described as the target of IgE in individuals suffering immediate hypersensitivity reactions to the novel anti-EGF monoclonal antibody cetuximab, it is now clear that α-gal sensitization is associated with mammalian meat allergy as well as reactions to other mammalian products...
January 2017: Current Allergy and Asthma Reports
https://www.readbyqxmd.com/read/28124083/-alpha-gal-in-therapeutics-more-relevant-than-thought
#20
B Wedi
No abstract text is available yet for this article.
March 2017: Der Hautarzt; Zeitschrift Für Dermatologie, Venerologie, und Verwandte Gebiete
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