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https://www.readbyqxmd.com/read/28380688/fabrication-and-characterization-of-a-decellularized-bovine-tendon-sheet-for-tendon-reconstruction
#1
Liang-Ju Ning, Yan-Lin Jiang, Cheng-Hao Zhang, Yi Zhang, Jie-Liang Yang, Jing Cui, Yan-Jing Zhang, Xuan Yao, Jing-Cong Luo, Ting-Wu Qin
Obtaining a performing decellularized tendon scaffold with proper dimensions and adequate availability is highly desirable. However, the combined study of complete decellularization and detailed characterization of native tendon extracellular matrix (ECM) from large animals is still lacking. In the present study, we developed a new decellularization protocol, including physical methods and enzymatic solutions for processing bovine Achilles tendons, and produced a decellularized bovine tendon sheet (DBTS) scaffold for tendon reconstruction...
April 5, 2017: Journal of Biomedical Materials Research. Part A
https://www.readbyqxmd.com/read/28360401/mutational-analysis-of-the-gla-gene-in-mexican-families-with-fabry-disease
#2
Bianca Ethel Gutiérrez-Amavizca, Andreas Gal, Rocío Ortíz-Orozco, Ulrich Orth, Ernesto Prado Montes De Oca, Jaime Paul Gutiérrez-Amavizca, Luis E Figuera
Fabry disease (FD) is a lysosomal storage disorder, which develops due to a deficiency in the hydrolytic enzyme, α-galactosidase A (α-Gal A). Alpha-Gal A hydrolyzes glycosphingolipid globotriaosylceramide (Gb3), and an α-Gal A deficiency leads to Gb3 accumulation in tissues and cells in the body. This pathology is likely to involve multiple systems, but it is generally considered to affect primarily vascular endothelium. In this study, we investigated mutations in the GLA gene, which encodes α-Gal A, in Mexican families with FD...
March 2017: Journal of Genetics
https://www.readbyqxmd.com/read/28294930/-the-alpha-gal-syndrome-an-allergic-reaction-to-mammalian-meat-secondary-to-a-tick-bite
#3
M A Berends, J N G Oude Elberink
Here we present the case of a 68-year-old patient with alpha-gal syndrome. This is a delayed-onset allergic reaction, characteristically occurring 2-6 hours after ingestion of mammalian meat products. The reaction occurs because the patient has developed IgE antibodies to a mammalian oligosaccharide epitope, galactose-α-1,3-galactose (alpha-gal); tick bites induce this IgE antibody response. Presentation varies from chronic urticaria to life-threatening anaphylaxis. The alpha-gal syndrome is usually self-limiting as long as there are no new tick bites...
2017: Nederlands Tijdschrift Voor Geneeskunde
https://www.readbyqxmd.com/read/28273338/prevalence-of-type-i-sensitization-to-alpha-gal-in-forest-service-employees-and-hunters
#4
Jörg Fischer, Elena Lupberger, Johanna Hebsaker, Gunnar Blumenstock, Elisabeth Aichinger, Amir S Yazdi, Doris Reick, Rainer Oehme, Tilo Biedermann
BACKGROUND: The production of IgE molecules specific to the carbohydrate galactose-α-1,3-galactose (alpha-gal) is known to induce delayed anaphylaxis against mammalian meat. Tick bites constitute the primary sensitization source, as ticks transfer alpha-gal in their saliva to a host during a bite. The reported prevalence of alpha-gal-specific IgE (alpha-gal-sIgE) positivity varies between different populations from diverse geographic regions. OBJECTIVE: To investigate the prevalence of alpha-gal-sIgE positivity in a population of forest service employees who are highly exposed to ticks in comparison with a residential population and a historic sample...
March 8, 2017: Allergy
https://www.readbyqxmd.com/read/28238108/enhanced-vascular-biocompatibility-of-decellularized-xeno-allogeneic-matrices-in-a-rodent-model
#5
M van Steenberghe, T Schubert, Y Guiot, C Bouzin, X Bollen, P Gianello
Glutaraldehyde preservation is the gold standard for cardiovascular biological prosthesis. However, secondary calcifications and the absence of tissue growth remain major limitations. Our study assessed in vitro and in vivo the biocompatibility of human (fascia lata, pericardium) and porcine tissues (pericardium, peritoneum) treated with a physicochemical procedure for decellularization and non-conventional pathogens inactivation. Biopsies were performed before and after treatment to assess decellularization (HE/Dapi staining/DNA quantification/MHC I/alpha gal immunostaining) and mechanical integrity...
February 25, 2017: Cell and Tissue Banking
https://www.readbyqxmd.com/read/28224342/galactose-%C3%AE-1-3-galactose-atypical-food-allergen-or-model-ige-hypersensitivity
#6
REVIEW
Jeffrey M Wilson, Alexander J Schuyler, Nikhila Schroeder, Thomas A E Platts-Mills
PURPOSE OF REVIEW: Galactose-α-1,3-galactose (α-gal) is a carbohydrate allergen with several unique characteristics. In this article, we discuss some recent advances in our understanding of the 'alpha-gal syndrome,' highlight data supporting the role of ticks in pathogenesis, and speculate on immune mechanisms that lead to sensitization. RECENT FINDINGS: First described as the target of IgE in individuals suffering immediate hypersensitivity reactions to the novel anti-EGF monoclonal antibody cetuximab, it is now clear that α-gal sensitization is associated with mammalian meat allergy as well as reactions to other mammalian products...
January 2017: Current Allergy and Asthma Reports
https://www.readbyqxmd.com/read/28124083/-alpha-gal-in-therapeutics-more-relevant-than-thought
#7
B Wedi
No abstract text is available yet for this article.
January 25, 2017: Der Hautarzt; Zeitschrift Für Dermatologie, Venerologie, und Verwandte Gebiete
https://www.readbyqxmd.com/read/27986511/anaphylaxis-after-zoster-vaccine-implicating-alpha-gal-allergy-as-a-possible-mechanism
#8
Cosby A Stone, Jonathan A Hemler, Scott P Commins, Alexander J Schuyler, Elizabeth J Phillips, R Stokes Peebles, John M Fahrenholz
No abstract text is available yet for this article.
December 14, 2016: Journal of Allergy and Clinical Immunology
https://www.readbyqxmd.com/read/27873733/food-allergy-to-the-carbohydrate-galactose-alpha-1-3-galactose-alpha-gal-four-case-reports-and-a-review
#9
Andreas J Bircher, Kathrin Scherer Hofmeier, Susanne Link, Ingmar Heijnen
Until recently, food allergies to mammalian meats have been considered to be very rare. The observation that patients not previously exposed to the monoclonal chimeric antibody cetuximab suffered from severe anaphylaxis upon first exposure, led to the identification of galactose-alpha-1,3-galactose as a new relevant carbohydrate allergen. These patients later often suffered from anaphylactic reactions to red meat. Epidemiological data indicated that bites by the tick Amblyomma americanum in the USA, later also by Ixodes species in other continents, resulted in sensitisation to alpha-gal...
February 1, 2017: European Journal of Dermatology: EJD
https://www.readbyqxmd.com/read/27834588/generation-of-ggta1-mutant-pigs-by-direct-pronuclear-microinjection-of-crispr-cas9-plasmid-vectors
#10
Chin-Kai Chuang, Chien-Hong Chen, Chung-Ling Huang, Yu-Hsiu Su, Shu-Hui Peng, Tai-Yun Lin, Hao-Chih Tai, Tien-Shuh Yang, Ching-Fu Tu
This study was conducted to confirm that 1-site and 4-site ppU6-GGTA1-gRNA CRISPR vectors together with the pCX-Flag2-NLS1-Cas9-NLS2 plasmid can both generate KO pigs by direct pronuclear microinjection. In total, 41 and 53 fertilized eggs were microinjected on 1-site and 4-site strategies, respectively. The 1-site construction generated a litter of 8 piglets, and 2 were mono-allelic mutant (mMt). The injection of 4-site constructions resulted in one biallelic mutant (bMt) and one mMt piglet in a litter of 7...
November 11, 2016: Animal Biotechnology
https://www.readbyqxmd.com/read/27806678/decellularization-and-characterization-of-porcine-superflexor-tendon-a-potential-anterior-cruciate-ligament-replacement
#11
Gemma Jones, Anthony Herbert, Helen Berry, Jennifer Helen Edwards, John Fisher, Eileen Ingham
The porcine superflexor tendon (SFT) was identified as having appropriate structure and properties for development of a decellularized device for use in anterior cruciate ligament reconstruction. SFTs were decellularized using a combination of freeze-thaw and washes in hypotonic buffer and 0.1% (w/v) sodium dodecyl sulfate in hypotonic buffer plus proteinase inhibitors, followed by nuclease treatment and sterilization using peracetic acid. The decellularized biological scaffold was devoid of cells and cell remnants and contained only 13 ng/mg (dry weight) residual total DNA...
February 2017: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/27775867/alpha-gal-is-a-possible-target-of-ige-mediated-reactivity-to-antivenom
#12
J Fischer, B Eberlein, C Hilger, F Eyer, S Eyerich, M Ollert, T Biedermann
BACKGROUND: Antivenoms are mammalian immunoglobulins with the ability to neutralize snake venom components and to mitigate the progression of toxic effects. Immediate hypersensitivity to antivenoms often occurs during the first administration of these heterologous antibodies. A comparable clinical situation occurred after introduction of cetuximab, a chimeric mouse-human antibody, for cancer treatment. The carbohydrate epitope galactose-alpha-1,3-galactose, located on the Fab region of cetuximab, was identified as the target responsible for IgE reactivity...
October 24, 2016: Allergy
https://www.readbyqxmd.com/read/27735906/-the-neurological-manifestations-of-fabry-disease-a-review
#13
K V Firsov, A S Kotov
Fabry disease (Anderson-Fabry disease) is an X-linked recessive lysosomal storage disorder resulting from deficient activity of lysosomal hydrolase, alpha-galactosidase A (alpha-Gal A), which leads to progressive accumulation of globotriaosylceramide (Gb3) in various cells, predominantly endothelial and vascular smooth muscle cells, with clinical manifestations affecting major organs including the central nervous system. Manifestations of Fabry disease include progressive renal and cardiac insufficiency, neuropathic pain, stroke and cerebral disease, skin and gastrointestinal symptoms...
2016: Zhurnal Nevrologii i Psikhiatrii Imeni S.S. Korsakova
https://www.readbyqxmd.com/read/27520937/invited-commentary-alpha-gal-allergy-tip-of-the-iceberg-to-a-pivotal-immune-response
#14
REVIEW
Scott P Commins
The syndrome of delayed allergic reactions to the carbohydrate galactose-alpha-1,3-galactose ("alpha-gal") has become increasingly recognized in allergy and immunology clinics regionally throughout the southeastern USA. Due to the increasing awareness of this unique food allergy, cases have been identified in the northeastern and central USA as well as in Central and South America, Europe, Asia, Scandinavia, and Australia. Clinically, alpha-gal allergy is characterized by reactions to non-primate mammalian meat (e...
September 2016: Current Allergy and Asthma Reports
https://www.readbyqxmd.com/read/27367933/the-cd25-binding-antibody-daclizumab-high-yield-process-has-a-distinct-glycosylation-pattern-and-reduced-antibody-dependent-cell-mediated-cytotoxicity-in-comparison-to-zenapax%C3%A2
#15
Bishu Ganguly, Balaji Balasa, Lyubov Efros, Paul R Hinton, Stephen Hartman, Archana Thakur, Joanna M Xiong, Brian Schmidt, Randy R Robinson, Thierry Sornasse, Vladimir Vexler, James P Sheridan
The CD25-binding antibody daclizumab high-yield process (DAC HYP) is an interleukin (IL)-2 signal modulating antibody that shares primary amino acid sequence and CD25 binding affinity with Zenapax®, a distinct form of daclizumab, which was approved for the prevention of acute organ rejection in patients receiving renal transplants as part of an immunosuppressive regimen that includes cyclosporine and corticosteroids. Comparison of the physicochemical properties of the two antibody forms revealed the glycosylation profile of DAC HYP differs from Zenapax in both glycan distribution and the types of oligosaccharides, most notably high-mannose, galactosylated and galactose-alpha-1,3-galactose (alpha-Gal) oligosaccharides, resulting in a DAC HYP antibody material that is structurally distinct from Zenapax...
July 1, 2016: MAbs
https://www.readbyqxmd.com/read/27356581/the-level-of-igg-antibodies-reactive-to-tf-tn-and-alpha-gal-polyacrylamide-glycoconjugates-in-breast-cancer-patients-relation-to-survival
#16
E P Smorodin, B L Sergeyev
BACKGROUND: The serum levels of IgG antibodies reactive to glycoconjugates (TF, Tn and αGal) were found to be associated with prognosis of gastrointestinal cancer patients. AIM: To study the relation between the levels of serum antibodies to TF-pAA, Tn-PAA and αGal-PAA polyacrylamide-based glycoconjugates and survival in breast cancer. MATERIALS AND METHODS: The preoperative level of IgG antibodies was analysed in the serum of patients (n = 59) using ELISA with polyacrylamide-glycoconjugates namely, TF-pAA (amide-type), and ethanolamide-conjugates Tn-PAA and αGal-PAA...
June 2016: Experimental Oncology
https://www.readbyqxmd.com/read/27310581/comparative-decellularization-and-recellularization-of-wild-type-and-alpha-1-3-galactosyltransferase-knockout-pig-lungs-a-model-for-ex-vivo-xenogeneic-lung-bioengineering-and-transplantation
#17
Joseph Platz, Nicholas R Bonenfant, Franziska E Uhl, Amy L Coffey, Tristan McKnight, Charles Parsons, Dino Sokocevic, Zachary D Borg, Ying-Wai Lam, Bin Deng, Julia G Fields, Michael DeSarno, Roberto Loi, Andrew M Hoffman, John Bianchi, Brian Dacken, Thomas Petersen, Darcy E Wagner, Daniel J Weiss
BACKGROUND: A novel potential approach for lung transplantation could be to utilize xenogeneic decellularized pig lung scaffolds that are recellularized with human lung cells. However, pig tissues express several immunogenic proteins, notably galactosylated cell surface glycoproteins resulting from alpha 1,3 galactosyltransferase (α-gal) activity, that could conceivably prevent effective use. Use of lungs from α-gal knock out (α-gal KO) pigs presents a potential alternative and thus comparative de- and recellularization of wild-type and α-gal KO pig lungs was assessed...
August 2016: Tissue Engineering. Part C, Methods
https://www.readbyqxmd.com/read/27264001/ige-in-the-diagnosis-and-treatment-of-allergic-disease
#18
Thomas A E Platts-Mills, Alexander J Schuyler, Elizabeth A Erwin, Scott P Commins, Judith A Woodfolk
Traditionally, the concept of allergy implied an abnormal response to an otherwise benign agent (eg, pollen or food), with an easily identifiable relationship between exposure and disease. However, there are syndromes in which the relationship between exposure to the relevant allergen and the "allergic" disease is not clear. In these cases the presence of specific IgE antibodies can play an important role in identifying the relevant allergen and provide a guide to therapy. Good examples include chronic asthma and exposure to perennial indoor allergens and asthma related to fungal infection...
June 2016: Journal of Allergy and Clinical Immunology
https://www.readbyqxmd.com/read/27238083/premature-bioprosthetic-aortic-valve-degeneration-associated-with-allergy-to-galactose-alpha-1-3-galactose
#19
Robert B Hawkins, Helena L Frischtak, Irving L Kron, Ravi K Ghanta
We present the cases of two patients with bioprosthetic aortic valves who developed an allergy to alpha-gal. Each had premature degeneration of their bioprosthesis and demonstrated rapidly increasing transvalvular gradients after development of their allergy. Each underwent successful replacement with a mechanical aortic valve within 1-2 years of symptom onset. doi: 10.1111/jocs.12764 (J Card Surg 2016;31:446-448).
July 2016: Journal of Cardiac Surgery
https://www.readbyqxmd.com/read/27164637/successful-desensitization-to-cetuximab-in-a-patient-with-a-positive-skin-test-to-cetuximab-and-specific-ige-to-alpha-gal
#20
J M García-Menaya, C Cordobés-Durán, J Gómez-Ulla, M A Zambonino, A C Mahecha, G M Chiarella, N Giangrande, P Bobadilla-González
No abstract text is available yet for this article.
2016: Journal of Investigational Allergology & Clinical Immunology
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