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Tubular albumin transcytosis

Fang-Fang He, Yi Gong, Zhen-Qiong Li, Liang Wu, Hua-Jun Jiang, Hua Su, Chun Zhang, Yu-Mei Wang
Transcytosis is an important intracellular transport process by which multicellular organisms selectively move cargoes from apical to basolateral membranes without disrupting cellular homeostasis. In kidney, macromolecular components in the serum, such as albumin, low-density lipoprotein and immunoglobulins, pass through the glomerular filtration barrier (GFB) and proximal tubular cells (PTCs) by transcytosis. Protein transcytosis plays a vital role in the pathology of albuminuria, which causes progressive destruction of the GFB structure and function...
June 15, 2018: Cellular Physiology and Biochemistry
Takahito Moriyama, Kazunori Karasawa, Kosaku Nitta
BACKGROUND: In the traditional theory of albuminuria, small amounts of albumin pass through the fenestrae in glomerular endothelial cells, then through the slit membrane in the gaps between foot processes of glomerular epithelial cells. In the novel theory, large amounts of albumin pass through glomerular capillaries and are taken up by megalin and cubilin receptors on tubular epithelial cells. These etiologies of urinary albumin excretion are still controversial, and the details of albumin passage through the three layers of glomerular capillaries (glomerular endothelial cells, basement membrane, and epithelial cells) have never been entirely elucidated...
2018: Contributions to Nephrology
Erik I Christensen, Henrik Birn
In a recent study using transgenic mice with inducible podocyte-specific expression of tagged albumin, Tenten and colleagues report transtubular transport of albumin, possibly mediated by the neonatal Fc receptor. This study raises several questions about the physiological importance of this potential pathway and the implications for albuminuria in renal disease.
December 2013: Nature Reviews. Nephrology
Verena Tenten, Sylvia Menzel, Uta Kunter, Eva-Maria Sicking, Claudia R C van Roeyen, Silja K Sanden, Michaela Kaldenbach, Peter Boor, Astrid Fuss, Sandra Uhlig, Regina Lanzmich, Brigith Willemsen, Henry Dijkman, Martin Grepl, Klemens Wild, Wilhelm Kriz, Bart Smeets, Jürgen Floege, Marcus J Moeller
Under physiologic conditions, significant amounts of plasma protein pass the renal filter and are reabsorbed by proximal tubular cells, but it is not clear whether the endocytosed protein, particularly albumin, is degraded in lysosomes or returned to the circulatory system intact. To resolve this question, a transgenic mouse with podocyte-specific expression of doxycycline-inducible tagged murine albumin was developed. To assess potential glomerular backfiltration, two types of albumin with different charges were expressed...
December 2013: Journal of the American Society of Nephrology: JASN
Ruben M Sandoval, Bruce A Molitoris
Kidney diseases involving urinary loss of large essential macromolecules, such as serum albumin, have long been thought to be caused by alterations in the permeability barrier comprised of podocytes, vascular endothelial cells, and a basement membrane working in unison. Data from our laboratory using intravital 2-photon microscopy revealed a more permeable glomerular filtration barrier (GFB) than previously thought under physiologic conditions, with retrieval of filtered albumin occurring in an early subset of cells called proximal tubule cells (PTC)(1,2,3)...
2013: Journal of Visualized Experiments: JoVE
Ruben M Sandoval, Mark C Wagner, Monica Patel, Silvia B Campos-Bilderback, George J Rhodes, Exing Wang, Sarah E Wean, Sherry S Clendenon, Bruce A Molitoris
Different laboratories recently reported incongruous results describing the quantification of albumin filtration using two-photon microscopy. We investigated the factors that influence the glomerular sieving coefficient for albumin (GSC(A)) in an effort to explain these discordant reports and to develop standard operating procedures for determining GSC(A). Multiple factors influenced GSC(A), including the kidney depth of image acquisition (10-20 μm was appropriate), the selection of fluorophore (probes emitting longer wavelengths were superior), the selection of plasma regions for fluorescence measurements, the size and molecular dispersion characteristics of dextran polymers if used, dietary status, and the genetic strain of rat...
March 2012: Journal of the American Society of Nephrology: JASN
Jakub Gburek, Krzysztof Gołąb, Katarzyna Juszczyńska
Albumin is the main protein of blood plasma, lymph, cerebrospinal fluid and interstitial fluid. The protein assists in many important body functions, including maintenance of proper colloidal osmotic pressure, transport of important metabolites and antioxidant action. Synthesis of albumin takes place mainly in the liver, and its catabolism occurs mostly in vascular endothelium of muscle, skin and liver as well as in the kidney tubular epithelium. Renal catabolism of albumin consists of glomerular filtration and tubular reabsorption...
2011: Postȩpy Higieny i Medycyny Doświadczalnej
L Megias, C Guerri, E Fornas, I Azorin, E Bendala, M Sancho-Tello, J M Durán, M Tomás, M J Gomez-Lechon, J Renau-Piqueras
Endocytosis constitutes an essential process in the regulation of the expression of cell surface molecules and receptors and, therefore, could participate in the neural-glial interactions occurring during brain development. However, the relationship between endocytic pathways in astroglial cells under physiological and pathological conditions remains poorly understood. We analyzed the endocytosis and transcytosis processes in growing astrocytes and the possible effect of ethanol on these processes. Evidence demonstrates that ethanol affects endocytosis in the liver and we showed that ethanol exposure during brain development alters astroglial development changing plasma membrane receptors and surface glycoprotein composition...
February 2000: International Journal of Developmental Biology
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