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https://www.readbyqxmd.com/read/28708585/the-neglected-avian-hepatotropic-virus-induces-acute-and-chronic-hepatitis-in-ducks-an-alternative-model-for-hepatology
#1
Xumin Ou, Sai Mao, Jingyu Cao, Yunchao Ma, Guangpeng Ma, Anchun Cheng, Mingshu Wang, Dekang Zhu, Shun Chen, Renyong Jia, Mafeng Liu, Kunfeng Sun, Qiao Yang, Ying Wu, Xiaoyue Chen
Duck Hepatitis A Virus (DHAV) belongs to the Avihepatovirus, which is also classified into Picornaviridae with Hepatovirus, Hepatitis A Virus (HAV). In humans, the pathogenesis of HAV is not well understood because of limited work with animal models. Here, we investigated the progress of duck viral hepatitis caused by DHAV and their potential for dissecting the pathogenesis of HAV. During the course of infection, the duck model had undergone hepatocellular lesions (vacuolation, acidophilic degeneration and steatosis), lymphocytes recruitment (neutrophil granulocytes, heterophilic granulocytes and T cells or plasm cells) and repair (activation of hepatic stellate cells, fibrosis and regeneration)...
July 5, 2017: Oncotarget
https://www.readbyqxmd.com/read/28660427/cholesterol-bilayer-domains-in-the-eye-lens-health-a-review
#2
Justyna Widomska, Witold K Subczynski, Laxman Mainali, Marija Raguz
The most unique biochemical characteristic of the eye lens fiber cell plasma membrane is its extremely high cholesterol content, the need for which is still unclear. It is evident, however, that the disturbance of Chol homeostasis may result in damages associated with cataracts. Electron paramagnetic resonance methods allow discrimination of two types of lipid domains in model membranes overloaded with Chol, namely, phospholipid-cholesterol domains and pure Chol bilayer domains. These domains are also detected in human lens lipid membranes prepared from the total lipids extracted from lens cortices and nuclei of donors from different age groups...
June 29, 2017: Cell Biochemistry and Biophysics
https://www.readbyqxmd.com/read/28655540/cloning-expression-pattern-and-promoter-functional-analysis-of-cyp19a1a-gene-in-miiuy-croaker
#3
Wei Huang, Pan Yang, Zhenming Lv, Changwen Wu, Jianfang Gui, Bao Lou
Gonadal-specific aromatase encoded by cyp19a1a is the important enzyme controlling estrogen biosynthesis in teleosts. In the present study, the cDNA sequence of cyp19a1a was cloned and characterized from miiuy croaker Miichthys miiuy. The cDNA encoded a protein of 519 amino acids with five structural regions. Higher identities of amino acid sequences and conserved structural regions were found between Mmcyp19a1a and other cyp19a1a genes. In addition, Mmcyp19a1a was clustered together with other seawater fishes...
June 24, 2017: Gene
https://www.readbyqxmd.com/read/28632173/gene-expression-profiling-reveals-potential-players-of-left-right-asymmetry-in-female-chicken-gonads
#4
Zhiyi Wan, Yanan Lu, Lei Rui, Xiaoxue Yu, Fang Yang, Chengfang Tu, Zandong Li
Most female birds develop only a left ovary, whereas males develop bilateral testes. The mechanism underlying this process is still not completely understood. Here, we provide a comprehensive transcriptional analysis of female chicken gonads and identify novel candidate side-biased genes. RNA-Seq analysis was carried out on total RNA harvested from the left and right gonads on embryonic day 6 (E6), E12, and post-hatching day 1 (D1). By comparing the gene expression profiles between the left and right gonads, 347 differentially expressed genes (DEGs) were obtained on E6, 3730 were obtained on E12, and 2787 were obtained on D1...
June 20, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28584112/causes-and-evolutionary-consequences-of-primordial-germ-cell-specification-mode-in-metazoans
#5
Carrie A Whittle, Cassandra G Extavour
In animals, primordial germ cells (PGCs) give rise to the germ lines, the cell lineages that produce sperm and eggs. PGCs form in embryogenesis, typically by one of two modes: a likely ancestral mode wherein germ cells are induced during embryogenesis by cell-cell signaling (induction) or a derived mechanism whereby germ cells are specified by using germ plasm-that is, maternally specified germ-line determinants (inheritance). The causes of the shift to germ plasm for PGC specification in some animal clades remain largely unknown, but its repeated convergent evolution raises the question of whether it may result from or confer an innate selective advantage...
June 6, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28536148/the-lotus-domain-is-a-conserved-dead-box-rna-helicase-regulator-essential-for-the-recruitment-of-vasa-to-the-germ-plasm-and-nuage
#6
Mandy Jeske, Christoph W Müller, Anne Ephrussi
DEAD-box RNA helicases play important roles in a wide range of metabolic processes. Regulatory proteins can stimulate or block the activity of DEAD-box helicases. Here, we show that LOTUS (Limkain, Oskar, and Tudor containing proteins 5 and 7) domains present in the germline proteins Oskar, TDRD5 (Tudor domain-containing 5), and TDRD7 bind and stimulate the germline-specific DEAD-box RNA helicase Vasa. Our crystal structure of the LOTUS domain of Oskar in complex with the C-terminal RecA-like domain of Vasa reveals that the LOTUS domain occupies a surface on a DEAD-box helicase not implicated previously in the regulation of the enzyme's activity...
May 1, 2017: Genes & Development
https://www.readbyqxmd.com/read/28479482/embryonic-expression-of-festina-lente-fel-a-novel-maternal-gene-in-the-oligochaete-annelid-tubifex-tubifex
#7
Takuma Nakamura, Inori Shiomi, Takashi Shimizu
We have cloned and characterized the expression of a novel maternal gene festina lente (designated Ttu-fel) from the clitellate annelid Tubifex tubifex. Northern blot analyses have shown that Ttu-fel mRNA is approximately 8 kbp in length and that its expression is restricted to oocytes undergoing maturation division and early embryos up to 22-cell stage. Maternal transcripts of Ttu-fel are first detected in oocytes in the ovary of young adults (ca. 40 days after hatching); its expression continues in growing oocytes in the ovisac...
May 4, 2017: Gene Expression Patterns: GEP
https://www.readbyqxmd.com/read/28397605/xenopus-vasa-homolog-xvlg1-is-essential-for-migration-and-survival-of-primordial-germ-cells
#8
Kazumi Shimaoka, Yoshiko Mukumoto, Yoko Tanigawa, Tohru Komiya
Xenopus vasa-like gene 1 (XVLG1), a DEAD-Box Helicase 4 (DDX4) gene identified as a vertebrate vasa homologue, is required for the formation of primordial germ cells (PGCs). However, it remains to be clarified when and how XVLG1 functions in the formation of the germ cells. To gain a better understanding of the molecular mechanisms underlying XVLG1 during PGC development, we injected XVLG1 morpholino oligos into germ-plasm containing blastomeres of 32-cell stage of Xenopus embryos, and traced cell fates of the injected blastomere-derived PGCs...
April 2017: Zoological Science
https://www.readbyqxmd.com/read/28396313/acquired-met-y1248h-and-d1246n-mutations-mediate-resistance-to-met-inhibitors-in-non-small-cell-lung-cancer
#9
An-Na Li, Jinji Yang, Xu-Chao Zhang, Zhou Zhang, Jian Su, Lan-Ying Gou, Yu Bai, Qing Zhou, Zhenfan Yang, Han Han-Zhang, Wenzhao Zhong, Shannon Chuai, Qi Zhang, Zhi Xie, Hong-Fei Gao, Hua-Jun Chen, Zheng Wang, Zhen Wang, Xue-Ning Yang, Bin-Chao Wang, Bin Gan, Zhihong Chen, Ben-Yuan Jiang, Si-Pei Wu, Si-Yang Liu, Chongrui Xu, Yi-Long Wu
Purpose: MET amplification, responsible for 20% of acquired resistance to epidermal growth factor receptor (EGFR) tyrosine kinase inhibitor (TKI) in patients with advanced non-small cell lung cancer (NSCLC), presents an attractive target. Numerous studies have conferred susceptibility of MET mutations and focal amplification to targeted MET-TKIs. However, the mechanism underlying MET-TKIs-induced resistance remains elusive. <p>Experimental Design: We conducted a cohort of 12 patients with advanced NSCLC who developed resistance to a combinatorial therapy consisting of gefitinib and a Type I MET-TKI...
April 10, 2017: Clinical Cancer Research: An Official Journal of the American Association for Cancer Research
https://www.readbyqxmd.com/read/28122234/germ-cell-less-promotes-centrosome-segregation-to-induce-germ-cell-formation
#10
Dorothy A Lerit, Conrad W Shebelut, Kristen J Lawlor, Nasser M Rusan, Elizabeth R Gavis, Paul Schedl, Girish Deshpande
The primordial germ cells (PGCs) specified during embryogenesis serve as progenitors to the adult germline stem cells. In Drosophila, the proper specification and formation of PGCs require both centrosomes and germ plasm, which contains the germline determinants. Centrosomes are microtubule (MT)-organizing centers that ensure the faithful segregation of germ plasm into PGCs. To date, mechanisms that modulate centrosome behavior to engineer PGC development have remained elusive. Only one germ plasm component, Germ cell-less (Gcl), is known to play a role in PGC formation...
January 24, 2017: Cell Reports
https://www.readbyqxmd.com/read/28115159/dnd-protein-functions-as-a-translation-repressor-during-zebrafish-embryogenesis
#11
Manami Kobayashi, Saori Tani-Matsuhana, Yasuka Ohkawa, Hiroshi Sakamoto, Kunio Inoue
Germline and somatic cell distinction is regulated through a combination of microRNA and germ cell-specific RNA-binding proteins in zebrafish. An RNA-binding protein, DND, has been reported to relieve the miR-430-mediated repression of some germ plasm mRNAs such as nanos3 and tdrd7 in primordial germ cells (PGCs). Here, we showed that miR-430-mediated repression is not counteracted by the overexpression of DND protein in somatic cells. Using a λN-box B tethering assay in the embryo, we found that tethering of DND to reporter mRNA results in translation repression without affecting mRNA stability...
March 4, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28087205/elevated-fasting-glucose-and-albuminuria-may-be-a-marker-for-all-cause-mortality-in-indigenous-adults-in-north-queensland-a-follow-up-study-1998-2006
#12
Ming Li, Robyn McDermott
AIMS: To document risk factors of all-cause mortality in a cohort of indigenous Australians from 23 communities of North Queensland during 1998-2006. METHODS: Among 2787 indigenous adults, baseline weight, waist circumference, blood pressure, fasting glucose, lipids, gamma-glutamyl transferase, urine albumin creatinine ratio, smoking, alcohol intake and physical activity were measured in 1998-2000. Deaths were ascertained from State Registry of Deaths, hospitalization and clinical records till 2006...
April 2017: Journal of Diabetes and its Complications
https://www.readbyqxmd.com/read/28065608/quantitative-differences-in-a-single-maternal-factor-determine-survival-probabilities-among-drosophila-germ-cells
#13
Maija Slaidina, Ruth Lehmann
Germ cell death occurs in many species [1-3] and has been proposed as a mechanism by which the fittest, strongest, or least damaged germ cells are selected for transmission to the next generation. However, little is known about how the choice is made between germ cell survival and death. Here, we focus on the mechanisms that regulate germ cell survival during embryonic development in Drosophila. We find that the decision to die is a germ cell-intrinsic process linked to quantitative differences in germ plasm inheritance, such that higher germ plasm inheritance correlates with higher primordial germ cell (PGC) survival probability...
January 23, 2017: Current Biology: CB
https://www.readbyqxmd.com/read/28013179/simultaneous-quantification-and-semi-quantification-of-ginkgolic-acids-and-their-metabolites-in-rat-plasma-by-uhplc-ltq-orbitrap-ms-and-its-application-to-pharmacokinetics-study
#14
Yiyun Qian, Zhenhua Zhu, Jin-Ao Duan, Sheng Guo, Erxin Shang, Jinhua Tao, Shulan Su, Jianming Guo
A highly sensitive method using ultra-high-pressure liquid chromatography coupled with linear ion trap-Orbitrap tandem mass spectrometry (UHPLC-LTQ-Orbitrap-MS) has been developed and validated for the simultaneous identification and quantification of ginkgolic acids and semi-quantification of their metabolites in rat plasma. For the five selected ginkgolic acids, the method was found to be with good linearities (r>0.9991), good intra- and inter-day precisions (RSD<15%), and good accuracies (RE, from -10...
January 15, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/28009314/hurtle-cells-immunohistochemical-activities-in-hashimoto-thyroiditis-parenchyma
#15
Z Tsagareli, T Kvachadze, E Melikadze, L Metreveli, E Nikobadze, L Gogiashvili
The present study was designed to evaluate the participation and utility of Hǘrtle cells morphological requirment and transformation under Hashimoto autoimmune thyroiditis versus Riedel´s struma. Several markers have been evaluated to detect induced activities of Hǘrtle cells. Study subject - specimens (tissue fragments) collected from TG surgery (thyroidectomy) for mollecular (receptor) diagnosis of Hǘrtle cells activities using routine histological and immunohistochemical samples. 89 cases were selected in Hashimoto thyroiditis diagnosis with Hǘrtle cells history (adenoma and adenomatous grouth of oncocytes)...
November 2016: Georgian Medical News
https://www.readbyqxmd.com/read/27975276/mechanisms-of-vertebrate-germ-cell-determination
#16
REVIEW
Tristan Aguero, Susannah Kassmer, Ramiro Alberio, Andrew Johnson, Mary Lou King
Two unique characteristics of the germ line are the ability to persist from generation to generation and to retain full developmental potential while differentiating into gametes. How the germ line is specified that allows it to retain these characteristics within the context of a developing embryo remains unknown and is one focus of current research. Germ cell specification proceeds through one of two basic mechanisms: cell autonomous or inductive. Here, we discuss how germ plasm driven germ cell specification (cell autonomous) occurs in both zebrafish and the frog Xenopus...
2017: Advances in Experimental Medicine and Biology
https://www.readbyqxmd.com/read/27836552/rhoa-rock-pathway-activity-is-essential-for-the-correct-localization-of-the-germ-plasm-mrnas-in-zebrafish-embryos
#17
Jerónimo Roberto Miranda-Rodríguez, Enrique Salas-Vidal, Hilda Lomelí, Mario Zurita, Denhi Schnabel
Zebrafish germ plasm is composed of mRNAs such as vasa and nanos and of proteins such as Bucky ball, all of which localize symmetrically in four aggregates at the distal region of the first two cleavage furrows. The coordination of actin microfilaments, microtubules and kinesin is essential for the correct localization of the germ plasm. Rho-GTPases, through their effectors, coordinate cytoskeletal dynamics. We address the participation of RhoA and its effector ROCK in germ plasm localization during the transition from two- to eight-cell embryos...
January 1, 2017: Developmental Biology
https://www.readbyqxmd.com/read/27802898/oxymatrine-attenuated-isoproterenol-induced-heart-failure-in-rats-via-regulation-of-cox-2-pgi2-pathway
#18
Ru Zhou, Qingbin Xu, Yehua Xu, Aiqin Xiong, Yang Wang, Ping Ma
Oxymatrine (OMT) is an active constituent of traditional Chinese herb Sophora japonica Ait which has been shown to exert potent anti-inflammatory,anti-oxidant and anti-fibrosis properties. Our previous studies have demonstrated that OMT has protective effects on isoproterenol-induced heart failure in rats through regulation of DDAH/ADMA metabolism pathway.In this study,we further investigated whether OMT could attenuate isoproterenol-induced heart failure through the regulation of COX-2/PGI2 pathway. Heart failure was induced in Sprague-Dawley rats by 5mg/kg isoproterenol subcutaneous injection for 7days...
December 2016: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/27801632/the-mrna-transportome-of-the-bicd-egl-transport-machinery
#19
Paula Vazquez-Pianzola, Bogdan Schaller, Martino Colombo, Dirk Beuchle, Samuel Neuenschwander, Anne Marcil, Rémy Bruggmann, Beat Suter
mRNA (mRNA) transport focuses the expression of encoded proteins to specific regions within cells providing them with the means to assume specific functions and even identities. BicD and the mRNA binding protein Egl interact with the microtubule motor dynein to localize mRNAs in Drosophila. Because relatively few mRNA cargos were known, we isolated and identified Egl::GFP associated mRNAs. The top candidates were validated by qPCR, in situ hybridization and genetically by showing that their localization requires BicD...
January 2, 2017: RNA Biology
https://www.readbyqxmd.com/read/27784263/multiplicity-of-buc-copies-in-atlantic-salmon-contrasts-with-loss-of-the-germ-cell-determinant-in-primates-rodents-and-axolotl
#20
Adrijana Škugor, Helge Tveiten, Hanne Johnsen, Øivind Andersen
BACKGROUND: The primordial germ cells (PGCs) giving rise to gametes are determined by two different mechanisms in vertebrates. While the germ cell fate in mammals and salamanders is induced by zygotic signals, maternally delivered germ cell determinants specify the PGCs in birds, frogs and teleost fish. Assembly of the germ plasm in the oocyte is organized by the single Buc in zebrafish, named Velo1 in Xenopus, and by Oskar in Drosophila. Secondary loss of oskar in several insect lineages coincides with changes in germline determination strategies, while the presence of buc in mammals suggests functions not associated with germline formation...
October 26, 2016: BMC Evolutionary Biology
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