Susanne Bentz, Theresa Pesch, Lutz Wolfram, Cheryl de Vallière, Katharina Leucht, Michael Fried, Johannes F Coy, Martin Hausmann, Gerhard Rogler
Transketolase-like (TKTL) 1 indirectly replenishes NADPH preventing damage induced by reactive oxygen species (ROS) formed upon intestinal inflammation. We investigated the function of TKTL1 during murine colitis and ROS detoxification for prevention of tissue damage. Mucosal damage in TKTL1(-/-) and wild-type (WT) mice was assessed by miniendoscopy and histology during dextran sodium sulfate (DSS) colitis. mRNA levels of interferon (IFN)-γ, inducible nitric oxide synthase (iNOS), interleukin (IL)-6, tumor necrosis factor (TNF), transketolase (TKT), and TKTL2 were determined by PCR and/or Western blotting...
April 2011: American Journal of Physiology. Gastrointestinal and Liver Physiology