Wei-Ju Chen, Wei-Kai Huang, Sarshan R Pather, Wei-Fang Chang, Li-Ying Sung, Han-Chung Wu, Mei-Ying Liao, Chi-Chiu Lee, Hsuan-Hui Wu, Chung-Yi Wu, Kuo-Shiang Liao, Chun-Yu Lin, Shang-Chih Yang, Hsuan Lin, Pei-Lun Lai, Chi-Hou Ng, Chun-Mei Hu, I-Chih Chen, Chi-Hsuan Chuang, Chien-Ying Lai, Po-Yu Lin, Yueh-Chang Lee, Scott C Schuyler, Axel Schambach, Frank Leigh Lu, Jean Lu
Deciphering signaling mechanisms critical for the extended pluripotent stem cell (EPSC) state and primed pluripotency is necessary for understanding embryonic development. Here, a membrane protein, podocalyxin-like protein 1 (PODXL) as being essential for extended and primed pluripotency, is identified. Alteration of PODXL expression levels affects self-renewal, protein expression of c-MYC and telomerase, and induced pluripotent stem cell (iPSC) and EPSC colony formation. PODXL is the first membrane protein reported to regulate de novo cholesterol biosynthesis, and human pluripotent stem cells (hPSCs) are more sensitive to cholesterol depletion than fibroblasts...
November 14, 2022: Advanced Science (Weinheim, Baden-Wurttemberg, Germany)