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Podocalyxin

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https://www.readbyqxmd.com/read/29677049/urinary-podocyte-associated-molecules-and-albuminuria-in-hypertension
#1
Javier Perez-Hernandez, Maria D Olivares, Elena Solaz, Fernando Martinez, Sergio Martínez-Hervas, Gernot Pichler, Felipe J Chaves, Josep Redon, Raquel Cortes
OBJECTIVE: Hypertension-induced podocyte damage and the relationship with UAE is analyzed in diabetic and nondiabetic participants. PATIENTS AND METHODS: Sixty-four hypertensive patients, 30 diabetics, with glomerular filtration rate (eGFR) greater than 60 ml/min per 1.73 m were included. Urinary albumin excretion was measured in morning urine using a nephelometric immunoassay and expressed as albumin/creatinine ratio. Urinary pellets were obtained from fresh urine and mRNA was assessed by real-time quantitative PCR...
April 19, 2018: Journal of Hypertension
https://www.readbyqxmd.com/read/29649375/anti-podocalyxin-monoclonal-antibody-47-mg-2a-detects-lung-cancers-by-immunohistochemistry
#2
Shinji Yamada, Shunsuke Itai, Mika K Kaneko, Yukinari Kato
Lung cancer is one of the leading causes of cancer-related deaths in the world. Regardless of the advances in lung cancer treatments, the prognosis is still poor. Podocalyxin (PODXL) is a highly glycosylated type I transmembrane protein that is expressed in normal tissues, including the heart, pancreas, and breast. It is also found and used as a diagnostic marker in many cancers, such as renal, brain, breast, oral, and lung cancers. We previously developed specific and sensitive anti-PODXL monoclonal antibodies, PcMab-47 (mouse IgG1 , kappa) and its mouse IgG2a -type (47-mG2a ), both of which were suitable for immunohistochemical analyses of oral cancers...
March 14, 2018: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy
https://www.readbyqxmd.com/read/29629277/weight-loss-has-an-additive-effect-on-the-proteinuria-reduction-of-angiotensin-ii-receptor-blockers-in-hypertensive-patients-with-chronic-kidney-disease
#3
Shin Young Ahn, Dong Ki Kim, Seung Seok Han, Jung Hwan Park, Sung Joon Shin, Sang Ho Lee, Bum Soon Choi, Chun Soo Lim, Suhnggwon Kim, Ho Jun Chin
Background: Weight reduction is a lifestyle intervention that has been introduced for prevention and management of chronic kidney disease (CKD). We investigate the additive anti-proteinuric effect of weight reduction on the usage of angiotensin II receptor blockers (ARBs) and its potential mechanisms in hypertensive CKD patients. Methods: This study is a subanalysis of data from an open-label, randomized, controlled clinical trial. Among the 235 participants, 227 were assigned to subgroups according to changes in body weight...
March 2018: Kidney Research and Clinical Practice
https://www.readbyqxmd.com/read/29604348/leukocyte-endothelial-cell-interaction-is-enhanced-in-podocalyxin-deficient-mice
#4
Gracia Porras, Matilde S Ayuso, Consuelo González-Manchón
The highly sialoglycosylated extracellular domain of podocalyxin (Podxl) is a constituent of the endothelial glycocalyx of most blood vessels but it is unknown if Podxl plays a prominent role in the function of the glycocalyx as a regulator of leukocyte-endothelial adhesion. We have recently found that mice lacking Podxl in the vascular endothelium develop histological lesions compatible with severe vasculitis resulting in organ failure and premature death. In this work, we show that these mice have an increased quantity of resident leukocytes within the peritoneal cavity in both basal and inflammatory conditions...
March 28, 2018: International Journal of Biochemistry & Cell Biology
https://www.readbyqxmd.com/read/29588598/measurement-of-serum-podxl-concentration-for-detection-of-pancreatic-cancer
#5
Keisuke Taniuchi, Makiko Tsuboi, Masahiko Sakaguchi, Toshiji Saibara
Background: The aim of this study was to investigate the use of podocalyxin (PODXL) and secretoglobin family 1D, member 2 (SCGB1D2) expressions in whole blood as diagnostic biomarkers to distinguish between patients with pancreatic cancer and control participants, in comparison with serum cancer antigen 19-9 (CA19-9), which is the current clinical standard. Patients and methods: Flow cytometric analysis was performed to determine the expressions of PODXL and SCGB1D2 on the surface of cultured pancreatic cancer cells...
2018: OncoTargets and Therapy
https://www.readbyqxmd.com/read/29567337/biomarkers-of-glomerular-dysfunction-in-pre-eclampsia-a-systematic-review
#6
REVIEW
Robert N Kerley, Cathal McCarthy
BACKGROUND: Early detection of pre-eclampsia remains one of the major focuses of antenatal obstetric care. There is often a delay in the diagnosis, mainly due to the non-specific nature of the condition. Podocytes which play a pivotal role in glomerular function become injured in pre-eclampsia leading to subsequent proteinuria. Our aim was to review available studies to determine the clinical utility of biomarkers of podocyte injury in pre-eclampsia. METHODS: We used QUADAS (Quality Assessment of Diagnostic Accuracy Studies) criteria to perform a systematic review of the literature to determine the clinical utility of podocyte injury biomarkers in predicting pre-eclampsia...
March 10, 2018: Pregnancy Hypertension
https://www.readbyqxmd.com/read/29554266/integration-of-molecules-to-construct-the-processes-of-conceptus-implantation-to-the-maternal-endometrium
#7
K Imakawa, R Bai, K Kusama
During the peri-implantation period, ruminant conceptuses go through rapid elongation, followed by their attachment to the uterine endometrial epithelial cells, during which interferon-tau (IFNT), a trophectodermal cytokine required for the process of maternal recognition of pregnancy, is expressed in a temporal and spatial manner. On d 22 (d 0 = day of estrus), 2 to 3 d after the initiation of bovine conceptus attachment to the uterine epithelium, when IFNT production begins to subside, the expression of molecules related to epithelial-mesenchymal transition, zinc finger E-box binding homeobox 1, snail family transcriptional repressor 2, N-cadherin and vimentin was found in the trophectoderm...
March 15, 2018: Journal of Animal Science
https://www.readbyqxmd.com/read/29540766/loss-of-ezrin-expression-reduced-the-susceptibility-to-the-glomerular-injury-in-mice
#8
Ryo Hatano, Ai Takeda, Yukiko Abe, Kotoku Kawaguchi, Itsuro Kazama, Mitsunobu Matsubara, Shinji Asano
Ezrin is highly expressed in glomerular podocytes and is reported to form a multi-protein complex with scaffold protein Na+ /H+ exchanger regulatory factor 2 (NHERF2) and podocalyxin, a major sialoprotein. Podocalyxin-knockout mice died within 24 h of birth with anuric renal failure, whereas NHERF2-knockout mice show no apparent changes in the glomerular functions. However, the physiological roles of ezrin in glomerular podocytes remain unclear. Here, we investigated the importance of ezrin in the regulation of glomerular podocyte function using ezrin-knockdown mice (Vil2kd/kd )...
March 14, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29463042/promotion-of-vascular-morphogenesis-of-endothelial-cells-co-cultured-with-human-adipose-derived-mesenchymal-stem-cells-using-polycaprolactone-gelatin-nanofibrous-scaffolds
#9
Yun-Min Kook, Hyerim Kim, Sujin Kim, Chan Yeong Heo, Min Hee Park, Kangwon Lee, Won-Gun Koh
New blood vessel formation is essential for tissue regeneration to deliver oxygen and nutrients and to maintain tissue metabolism. In the field of tissue engineering, in vitro fabrication of new artificial vessels has been a longstanding challenge. Here we developed a technique to reconstruct a microvascular system using a polycaprolactone (PCL)/gelatin nanofibrous structure and a co-culture system. Using a simple electrospinning process, we fabricated three-dimensional mesh scaffolds to support the sprouting of human umbilical vein endothelial cells (HUVECs) along the electrospun nanofiber...
February 18, 2018: Nanomaterials
https://www.readbyqxmd.com/read/29436620/highglucose-induces-podocyteepithelial%C3%A2-to%C3%A2-mesenchymal-transition-by-demethylation%C3%A2-mediated-enhancement-of-mmp9-expression
#10
Li Ling, Libo Chen, Changning Zhang, Shuyan Gui, Haiyan Zhao, Zhengzhang Li
Abnormal expression of matrix metalloproteinase 9 (MMP9) is correlated with podocyte epithelial-to---mesenchymal transition (EMT) in diabetic nephropathy (DN). However, the mechanisms underlying this process are not well defined. Site‑specific demethylation may sustain high expression levels of target genes. In the present study, in order to investigate the association between DNA demethylation of MMP9 promoter and podocyte EMT in DN, human podocytes were cultured in high‑glucose (HG) medium and a rat model of DN was established by intraperitoneal injection of streptozotocin (STZ) to determine whether site‑specific demethylation of the MMP9 promoter was involved in regulating podocyte EMT in DN...
February 2, 2018: Molecular Medicine Reports
https://www.readbyqxmd.com/read/29436579/long-noncoding-rna-ensrnog00000037522-is-involved-in-the-podocyte-epithelial%C3%A2-mesenchymal-transition-in-diabetic-rats
#11
Li Ling, Zhen Tan, Changning Zhang, Shuyan Gui, Yuanyuan Hu, Libo Chen
Diabetic nephropathy (DN) is one of the most common complications associated with type I and II diabetes mellitus. Long noncoding RNAs (lncRNAs) have been implicated in various physiological and pathological processes, and recent evidence has demonstrated that they are involved in the process of the epithelial‑mesenchymal transition (EMT). In the present study, the potential functions of lncRNA ENSRNOG00000037522 during the EMT process in DN were investigated. The results identified that the level of the lncRNA ENSRNOG00000037522 was significantly increased in kidney tissues collected from rats with streptozocin (STZ)‑induced DN accompanied by impairment of the glomerular podocytes...
February 5, 2018: International Journal of Molecular Medicine
https://www.readbyqxmd.com/read/29362381/gene-expression-of-human-endometrial-l-selectin-ligand-in-relation-to-the-phases-of-the-natural-menstrual-cycle
#12
Tsung-Hsuan Lai, Fung-Wei Chang, Jun-Jie Lin, Qing-Dong Ling
This study investigates peptide components of L-selectin ligand (LSL) and their gene expressions in human endometrium during the natural menstrual cycle. We recruited 41 endometrial samples from reproductive-aged women with leiomyoma and undergoing hysterectomy and 11 endometrial samples from menopausal women as controls. Immunohistochemistry revealed strong MECA-79 expression from the early through the mid-secretory phase and low expression in menopausal endometrium. Five peptide components of LSL were detected in reproductive and menopausal endometrium by one-step quantitative RT-PCR: podocalyxin, endomucin, nepmucin, GlyCAM-1, and CD34...
January 23, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29328453/aldosterone-is-involved-in-the-pathogenesis-of-obesity-related-glomerulopathy-through-activation-of-wnt-%C3%AE-catenin-signaling-in-podocytes
#13
Jia-Jia Zhu, Yi-Pu Chen, Min Yang, Bao-Li Liu, Jing Dong, Hong-Rui Dong, Hong-Liang Rui, Hong Cheng
Obesity-related glomerulopathy (ORG) is morphologically characterized by glomerulomegaly with or without observable focal segmental glomerulosclerosis under light microscope, with decreased podocyte density and number, and with increased foot‑process width observed under electron microscope. The severity of podocyte injury is correlated with the degree of proteinuria and renal dysfunction. However, the pathogenesis of ORG is not well understood. The aim of the present study was to explore the possible pathogenic role of aldosterone (ALDO) in ORG...
March 2018: Molecular Medicine Reports
https://www.readbyqxmd.com/read/29321582/serum-podocalyxin-levels-correlate-with-carotid-intima-media-thickness-implicating-its-role-as-a-novel-biomarker-for-atherosclerosis
#14
Mayumi Shoji, Minoru Takemoto, Kazuki Kobayashi, Toshihiro Shoji, Satoka Mori, Jun-Ichi Sagara, Hiroyuki Kurosawa, Yoshiaki Hirayama, Kenichi Sakamoto, Takahiro Ishikawa, Masaya Koshizaka, Yoshiro Maezawa, Koutaro Yokote
Podocalyxin is a cell surface sialomucin, which is expressed in not only glomerular podocytes but also vascular endothelial cells. Urinary podocalyxin is used as a marker for glomerular disease. However, there are no reports describing serum podocalyxin (s-Podxl) levels. Therefore, the association between s-Podxl levels and clinical parameters were examined with 52 patients. s-Podxl level was evaluated using enzyme-linked immunosorbent assay. The median s-Podxl level was 14.2 ng/dL (interquartile range: 10...
January 10, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29279420/deregulated-profiles-of-urinary-micrornas-may-explain-podocyte-injury-and-proximal-tubule-dysfunction-in-normoalbuminuric-patients-with-type-2-diabetes-mellitus
#15
Oana Milas, Florica Gadalean, Adrian Vlad, Victor Dumitrascu, Cristina Gluhovschi, Gheorghe Gluhovschi, Silvia Velciov, Roxana Popescu, Flaviu Bob, Petru Matusz, Agneta-Maria Pusztai, Octavian M Cretu, Alina Secara, Anca Simulescu, Sorin Ursoniu, Daliborca Vlad, Ligia Petrica
MicroRNAs (miRNAs) are short non-coding RNA species that are important post-transcriptional regulators of gene expression. The aim of the study was to establish a potential explanation of podocyte damage and proximal tubule (PT) dysfunction induced by deregulated miRNAs expression in the course of type 2 diabetes mellitus (DM). A total of 68 patients with type 2 DM and 11 healthy subjects were enrolled in a cross-sectional study and assessed concerning urinary albumin:creatinine ratio (UACR), urinary N -acetyl-β-D-glucosamininidase (NAG), urinary kidney injury molecule-1, urinary nephrin, podocalyxin, synaptopodin, estimated glomerular filtration rate (eGFR), urinary miRNA21, miRNA124, and miRNA192...
December 25, 2017: Journal of Investigative Medicine: the Official Publication of the American Federation for Clinical Research
https://www.readbyqxmd.com/read/29247494/podocyte-and-endothelial-cell-injury-lead-to-nephrotic-syndrome-in-proliferative-lupus-nephritis
#16
Aya Nawata, Satoshi Hisano, Shohei Shimajiri, Ke-Yong Wang, Yoshiya Tanaka, Toshiyuki Nakayama
AIMS: Nephrotic syndrome (NS) is a major manifestation of lupus nephritis (LN). The dysregulation of podocytes, the glomerular basement membrane (GBM) and endothelial cells (ECs) results in proteinuria in glomerular diseases. The aim of our study was to clarify whether the dysregulation of these barriers is associated with NS in proliferative LN and membranous LN. METHODS AND RESULTS: Fifty-six patients with NS, including minimal change NS in 15, primary membranous nephropathy (PMN) in 13, class III/IV LN in 15, and class V LN in 13, were enrolled in this study...
December 15, 2017: Histopathology
https://www.readbyqxmd.com/read/29245936/podocalyxin-promotes-proliferation-and-survival-in-mature-b-cell-non-hodgkin-lymphoma-cells
#17
Estíbaliz Tamayo-Orbegozo, Laura Amo, Marta Riñón, Naiara Nieto, Elena Amutio, Natalia Maruri, Miren Solaun, Arantza Arrieta, Susana Larrucea
Podocalyxin (PCLP1) is a CD34-related sialomucin expressed by some normal cells and a variety of malignant tumors, including leukemia, and associated with the most aggressive cancers and poor clinical outcome. PCLP1 increases breast tumor growth, migration and invasion; however, its role in hematologic malignancies still remains undetermined. The purpose of this study was to investigate the expression and function of PCLP1 in mature B-cell lymphoma cells. We found that overexpression of PCLP1 significantly increases proliferation, cell-to-cell interaction, clonogenicity, and migration of B-cell lymphoma cells...
November 21, 2017: Oncotarget
https://www.readbyqxmd.com/read/29244787/loss-of-podocalyxin-causes-a-novel-syndromic-type-of-congenital-nephrotic-syndrome
#18
Hee Gyung Kang, Moses Lee, Kyoung Boon Lee, Michael Hughes, Bo Sang Kwon, Sangmoon Lee, Kelly M McNagny, Yo Han Ahn, Jung Min Ko, Il-Soo Ha, Murim Choi, Hae Il Cheong
Many cellular structures directly imply specific biological functions. For example, normal slit diaphragm structures that extend from podocyte foot processes ensure the filtering function of renal glomeruli. These slits are covered by a number of surface proteins, such as nephrin, podocin, podocalyxin and CD2AP. Here we report a human patient presenting with congenital nephrotic syndrome, omphalocele and microcoria due to two loss-of-function mutations in PODXL, which encodes podocalyxin, inherited from each parent...
December 15, 2017: Experimental & Molecular Medicine
https://www.readbyqxmd.com/read/29187370/ste20-like-kinase-slk-a-novel-mediator-of-podocyte-integrity
#19
Andrey V Cybulsky, Joan Papillon, Julie Guillemette, Natalya Belkina, Genaro Patino-Lopez, Elena Torban
SLK is essential for embryonic development, and may play a key role in wound healing, tumor growth and metastasis. Expression and activation of SLK is increased in kidney development and during recovery from ischemic acute kidney injury. Overexpression of SLK in glomerular epithelial cells/podocytes in vivo induces injury and proteinuria. Conversely, reduced SLK expression leads to abnormalities in cell adhesion, spreading, and motility. Tight regulation of SLK expression thus may be critical for normal renal structure and function...
November 29, 2017: American Journal of Physiology. Renal Physiology
https://www.readbyqxmd.com/read/29050536/the-correlation-of-urinary-podocytes-and-podocalyxin-with-histological-features-of-lupus-nephritis
#20
D Ikuma, K Hiromura, H Kajiyama, J Suwa, H Ikeuchi, T Sakairi, Y Kaneko, A Maeshima, H Kurosawa, Y Hirayama, K Yokota, Y Araki, K Sato, Y F Asanuma, Y Akiyama, M Hara, Y Nojima, T Mimura
Objectives The objective of this study was to test the correlation of urinary podocyte number (U-Pod) and urinary podocalyxin levels (U-PCX) with histology of lupus nephritis. Methods This was an observational, cross-sectional study. Sixty-four patients were enrolled: 40 with lupus nephritis and 24 without lupus nephritis (12 lupus nephritis patients in complete remission and 12 systemic lupus erythematosus patients without lupus nephritis). Urine samples were collected before initiating treatment. U-Pod was determined by counting podocalyxin-positive cells, and U-PCX was measured by sandwich ELISA, normalized to urinary creatinine levels (U-Pod/Cr, U-PCX/Cr)...
March 2018: Lupus
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