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Podocalyxin

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https://www.readbyqxmd.com/read/29187370/ste20-like-kinase-slk-a-novel-mediator-of-podocyte-integrity
#1
Andrey V Cybulsky, Joan Papillon, Julie Guillemette, Natalya Belkina, Genaro Patino-Lopez, Elena Torban
SLK is essential for embryonic development, and may play a key role in wound healing, tumor growth and metastasis. Expression and activation of SLK is increased in kidney development and during recovery from ischemic acute kidney injury. Overexpression of SLK in glomerular epithelial cells/podocytes in vivo induces injury and proteinuria. Conversely, reduced SLK expression leads to abnormalities in cell adhesion, spreading, and motility. Tight regulation of SLK expression thus may be critical for normal renal structure and function...
November 29, 2017: American Journal of Physiology. Renal Physiology
https://www.readbyqxmd.com/read/29050536/the-correlation-of-urinary-podocytes-and-podocalyxin-with-histological-features-of-lupus-nephritis
#2
D Ikuma, K Hiromura, H Kajiyama, J Suwa, H Ikeuchi, T Sakairi, Y Kaneko, A Maeshima, H Kurosawa, Y Hirayama, K Yokota, Y Araki, K Sato, Y F Asanuma, Y Akiyama, M Hara, Y Nojima, T Mimura
Objectives The objective of this study was to test the correlation of urinary podocyte number (U-Pod) and urinary podocalyxin levels (U-PCX) with histology of lupus nephritis. Methods This was an observational, cross-sectional study. Sixty-four patients were enrolled: 40 with lupus nephritis and 24 without lupus nephritis (12 lupus nephritis patients in complete remission and 12 systemic lupus erythematosus patients without lupus nephritis). Urine samples were collected before initiating treatment. U-Pod was determined by counting podocalyxin-positive cells, and U-PCX was measured by sandwich ELISA, normalized to urinary creatinine levels (U-Pod/Cr, U-PCX/Cr)...
January 1, 2017: Lupus
https://www.readbyqxmd.com/read/29021382/sustained-activation-of-guanylate-cyclase-a-with-tdt-a-natriuretic-peptide-derivative-exhibits-cardiorenal-protection-in-dahl-salt-sensitive-hypertensive-rats
#3
Shohei Oishi, Naoko Suzuki, Yuri Hasui, Tsuyoshi Homma, Masanori Obana, Takahiro Nagayama, Yasushi Fujio
Heart failure often presents with prognosis-relevant impaired renal function. To investigate whether the chronic activation of guanylate cyclase-A (GC-A) protects both heart and kidney, we examined the effects of TDT, a neprilysin (NEP)-resistant natriuretic peptide (NP) derivative, on cardiac and renal dysfunction in Dahl salt-sensitive hypertensive rats (DS rats). Pretreatment with NEP or NEP inhibitor did not influence GC-A activation by TDT both in vitro and in vivo, resulting in a long-acting profile of TDT compared to native human atrial NP (hANP)...
October 11, 2017: Journal of Pharmacology and Experimental Therapeutics
https://www.readbyqxmd.com/read/28980094/podocalyxin-as-a-major-pluripotent-marker-and-novel-keratan-sulfate-proteoglycan-in-human-embryonic-and-induced-pluripotent-stem-cells
#4
REVIEW
Hidenao Toyoda, Yuko Nagai, Aya Kojima, Akiko Kinoshita-Toyoda
Podocalyxin (PC) was first identified as a heavily sialylated transmembrane protein of glomerular podocytes. Recent studies suggest that PC is a remarkable glycoconjugate that acts as a universal glyco-carrier. The glycoforms of PC are responsible for multiple functions in normal tissue, human cancer cells, human embryonic stem cells (hESCs), and human induced pluripotent stem cells (hiPSCs). PC is employed as a major pluripotent marker of hESCs and hiPSCs. Among the general antibodies for human PC, TRA-1-60 and TRA-1-81 recognize the keratan sulfate (KS)-related structures...
October 5, 2017: Glycoconjugate Journal
https://www.readbyqxmd.com/read/28953590/vascular-endothelium-a-potential-source-of-podocalyxin-in-serum-from-pregnancies-with-preeclampsia
#5
Styliani Goulopoulou
No abstract text is available yet for this article.
November 2017: Journal of Hypertension
https://www.readbyqxmd.com/read/28946619/podocalyxin-like-protein-1-regulates-taz-signaling-and-stemness-properties-in-colon-cancer
#6
Wen-Ying Lee, Chih-Chia Kuo, Bo-Xing Lin, Chia-Hsiung Cheng, Ku-Chung Chen, Cheng-Wei Lin
Colon cancer is the third most common cancer in the world and the second most common cause of cancer-related mortality. Molecular biomarkers for colon cancer have undergone vigorous discovery and validation. Recent studies reported that overexpression of podocalyxin-like protein 1 (PODXL) is associated with distant metastasis and poor prognosis across several types of malignancies. Its role and underlying molecular mechanism, however, are not yet fully understood. In the present study, we revealed that the Hippo transducer, the transcriptional coactivator with PDZ-binding motif (TAZ), acts as a downstream mediator of PODXL in colon cancer...
September 23, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28905451/gene-edited-human-kidney-organoids-reveal-mechanisms-of-disease-in-podocyte-development
#7
Yong Kyun Kim, Ido Refaeli, Craig R Brooks, Peifeng Jing, Ramila E Gulieva, Michael R Hughes, Nelly M Cruz, Yannan Liu, Angela J Churchill, Yuliang Wang, Hongxia Fu, Jeffrey W Pippin, Lih Y Lin, Stuart J Shankland, A Wayne Vogl, Kelly M McNagny, Benjamin S Freedman
A critical event during kidney organogenesis is the differentiation of podocytes, specialized epithelial cells that filter blood plasma to form urine. Podocytes derived from human pluripotent stem cells (hPSC-podocytes) have recently been generated in nephron-like kidney organoids, but the developmental stage of these cells and their capacity to reveal disease mechanisms remains unclear. Here, we show that hPSC-podocytes phenocopy mammalian podocytes at the capillary loop stage (CLS), recapitulating key features of ultrastructure, gene expression, and mutant phenotype...
December 2017: Stem Cells
https://www.readbyqxmd.com/read/28881743/prognostic-role-of-podocalyxin-like-protein-expression-in-various-cancers-a-systematic-review-and-meta-analysis
#8
Jing Wang, Yongzhao Zhao, Ruizhao Qi, Xiaoning Zhu, Chenshen Huang, Sijin Cheng, Shengzhi Wang, Xiaolong Qi
Several studies were conducted to explore the prognostic significance of podocalyxin-like protein (PODXL) expression in various cancers, with contradictory. This study aims to summarize the prognostic significance of PODXL expression in cancers. PubMed, the Cochrane Library and Embase were completely retrieved. The prospective or retrospective studies focusing on the prognostic role of PODXL expression in cancers were eligible. The endpoints were overall survival (OS), disease-specific survival (DSS) and disease-free survival (DFS)...
August 8, 2017: Oncotarget
https://www.readbyqxmd.com/read/28873000/immunohistochemical-analysis-using-antipodocalyxin-monoclonal-antibody-pcmab-47-demonstrates-podocalyxin-expression-in-oral-squamous-cell-carcinomas
#9
Shunsuke Itai, Shinji Yamada, Mika K Kaneko, Hiroyuki Harada, Yukinari Kato
Podocalyxin is a CD34-related type I transmembrane protein that is highly glycosylated with N-glycan, O-glycan, and keratan sulfate. Podocalyxin was originally found in the podocytes of rat kidney and is reportedly expressed in many types of tumors, including brain tumors, colorectal cancers, and breast cancers. Overexpression of podocalyxin is an independent predictor of progression, metastasis, and poor outcome. We recently immunized mice with recombinant human podocalyxin, which was produced using LN229 glioblastoma cells, and produced a novel antipodocalyxin monoclonal antibody (mAb), PcMab-47, which reacts with endogenous podocalyxin-expressing cancer cell lines and normal cell lines independent of glycosylation in Western blot, flow cytometry, and immunohistochemical analyses...
October 2017: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy
https://www.readbyqxmd.com/read/28871938/-shendi-granules-regulates-the-balance-of-t-cell-subsets-and-down-regulates-podocalyxin-in-rats-with-mesangial-proliferative-glomerulonephritis
#10
Yiping Wang, Lei Zhang, Dong Wang, Ling Wei, Hua Jin, Xuelian Zhang, Lan Hu
Objective To observe the effect of Shendi granules on T cell subsets and podocyte marker protein in rats with mesangial proliferative glomerulonephritis (MsPGN), and study possible mechanism. Methods Totally 40 SD rats were randomly divided into the model group, valsartan group, Shendi granule group and normal group. The Shendi granule group were given 4 g/(kg.d) of Shendi granule by gavage; the valsartan group were given 10.3 mg/(kg.d) of valsartan by gavage; the model group and normal group were given the same amount of saline per day by gavage...
August 2017: Xi Bao Yu Fen Zi Mian Yi Xue za Zhi, Chinese Journal of Cellular and Molecular Immunology
https://www.readbyqxmd.com/read/28842487/loss-of-mucin-type-o-glycans-impairs-the-integrity-of-the-glomerular-filtration-barrier-in-the-mouse-kidney
#11
Kai Song, Jianxin Fu, Jianhua Song, Brett H Herzog, Kirk Bergstrom, Yuji Kondo, J Michael McDaniel, Samuel McGee, Robert Silasi-Mansat, Florea Lupu, Hong Chen, Harini Bagavant, Lijun Xia
The kidney's filtration activity is essential for removing toxins and waste products from the body. The vascular endothelial cells of the glomerulus are fenestrated, flattened, and surrounded by podocytes, specialized cells that support glomerular endothelial cells. Mucin-type core 1-derived O-glycans (O-glycans) are highly expressed on both glomerular capillary endothelial cells and their supporting podocytes, but their biological role is unclear. Biosynthesis of core 1-derived O-glycans is catalyzed by the glycosyltransferase core 1 β1,3-galactosyltransferase (C1galt1)...
October 6, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28757276/value-of-immunohistochemical-expression-of-podocalyxin-in-active-lupus-nephritis
#12
Maha Abdelmoneim Behairy, Marwa Mosaad Shakweer, Tamer Wahid El Said, Nermin Hassan ElGharbawy
Podocalyxin is an electronegative sialoglycoprotein that prevents the podocyte foot process from collapsing. The aim of this study was to detect an association between the glomerular immunohistochemical (IHC) expression of podocalyxin and the degree of podocyte effacement detected by electron microscopy, and to evaluate the role of podocalyxin IHC expression as a novel marker for disease activity in lupus nephritis (LN). METHODS: Thirty-two renal biopsies of active lupus nephritis patients were studied...
July 27, 2017: Nefrología: Publicación Oficial de la Sociedad Española Nefrologia
https://www.readbyqxmd.com/read/28747404/galectin-8-regulates-targeting-of-gp135-podocalyxin-and-lumen-formation-at-the-apical-surface-of-renal-epithelial-cells
#13
HooiCheng Lim, Chun-Ying Yu, Tzuu-Shuh Jou
Establishment of apical-basal polarity, through correct targeting of polarity determinants to distinct domains of the plasma membrane, is a fundamental process for the development of functioning epithelial tubules. Here we report that galectin (Gal)-8 regulates apical-basal polarity of Madin-Darby canine kidney (MDCK) cells via apical targeting of 135-kDa glycoprotein (Gp135). Gal-8 interacts with newly synthesized Gp135 in a glycan-dependent manner. Gal-8 knockdown induces aberrant lumens at the lateral domain and mistargeting of Gp135 to this structure, thus disrupting the kidney epithelial polarity of MDCK cells, which organize lumens at the apical surface...
July 26, 2017: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
https://www.readbyqxmd.com/read/28665887/serum-podocalyxin-is-significantly-increased-in-early-onset-preeclampsia-and-may-represent-a-novel-marker-of-maternal-endothelial-cell-dysfunction
#14
Qi Chen, Yao Wang, Ying Li, Min Zhao, Guiying Nie
OBJECTIVE: Podocalyxin is a glomerular podocyte protein and increased in urine of preeclampsia. However, podocalyxin is also expressed in endothelial cells of other organs. Here we investigated whether podocalyxin is detectable in pregnant serum and whether the levels are altered in preeclampsia. METHODS: Podocalyxin was determined by ELISA in sera collected from normal pregnancy across gestation (n = 44) and from preeclamptic pregnancies at diagnosis (n = 34) with gestation-age-matched controls (n = 68)...
November 2017: Journal of Hypertension
https://www.readbyqxmd.com/read/28665782/antipodocalyxin-antibody-chpcmab-47-exerts-antitumor-activity-in-mouse-xenograft-models-of-colorectal-adenocarcinomas
#15
Mika K Kaneko, Akiko Kunita, Shinji Yamada, Takuro Nakamura, Miyuki Yanaka, Noriko Saidoh, Yao-Wen Chang, Saori Handa, Satoshi Ogasawara, Tomokazu Ohishi, Shinji Abe, Shunsuke Itai, Hiroyuki Harada, Manabu Kawada, Yasuhiko Nishioka, Masashi Fukayama, Yukinari Kato
Podocalyxin (PODXL) is expressed in several cancers, including brain tumors and colorectal cancers. PODXL overexpression is an independent predictor of progression, metastasis, and poor outcome. We recently immunized mice with recombinant human PODXL, which was produced using LN229 glioblastoma cells, and produced a clone PcMab-47 that could be used for investigating PODXL expression by flow cytometry and immunohistochemical analysis. Herein, we produced a human-mouse chimeric PcMab-47 (chPcMab-47) and investigated its antitumor activity against PODXL-expressing tumors...
August 2017: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy
https://www.readbyqxmd.com/read/28664547/vitamin-d3-ameliorates-podocyte-injury-through-the-nephrin-signalling-pathway
#16
Ourania Trohatou, Effie-Fotini Tsilibary, Aristidis Charonis, Christos Iatrou, Garyfalia Drossopoulou
Renal podocytes form the main filtration barrier possessing unique phenotype maintained by proteins including podocalyxin and nephrin, which are modulated in pathological conditions. In diabetic nephropathy (DN), podocytes become structurally and functionally compromised. Nephrin, a structural backbone protein of the slit diaphragm, acts as regulator of podocyte intracellular signalling with renoprotective role. Vitamin D3 through its receptor, VDR, provides renal protection in DN but limited data exist about its effect on podocytes...
October 2017: Journal of Cellular and Molecular Medicine
https://www.readbyqxmd.com/read/28439668/urinary-podocalyxin-as-a-possible-novel-marker-of-intrauterine-nephrogenesis-and-extrauterine-podocyte-injury
#17
Taihei Hayashi, Shuko Tokuriki, Takashi Okuno, Genrei Ohta, Aiko Igarashi, Yusei Ohshima
BACKGROUND: The number of nephrons at birth is determined during fetal development and is modulated thereafter by postnatal podocyte injury. Hyperfiltration, caused by a reduced number of nephrons, is a risk factor for chronic kidney disease. It is therefore important to monitor the formation of nephrons. METHODS: Urine samples were collected from infants within 1-2 days of birth, with follow-up sampling for preterm infants at 37-39 weeks of corrected age. Urinary levels of podocalyxin (PCX), β2-microglobulin (β2MG), N-acetyl-ß-D-glucosaminidase (NAG), total protein (TP), microalbumin (mAlb) and creatinine were measured and the relationship between these markers evaluated...
April 25, 2017: Pediatric Nephrology: Journal of the International Pediatric Nephrology Association
https://www.readbyqxmd.com/read/28408238/novel-roles-for-podocalyxin-in-regulating-stress-myelopoiesis-rap1a-and-neutrophil-migration
#18
Pan Li, Aldona A Karaczyn, Rose McGlauflin, Amanda J Favreau-Lessard, Edward Jachimowicz, Calvin P Vary, Kailin Xu, Don M Wojchowski, Pradeep Sathyanarayana
Podocalyxin (Podxl) is a CD34 orthologue and cell surface sialomucin reported to have roles in renal podocyte diaphragm slit development; vascular cell integrity; and the progression of blood, breast, and prostate cancers. Roles for Podxl during nonmalignant hematopoiesis, however, are largely undefined. We have developed a Vav-Cre Podxl knockout (KO) mouse model, and report on novel roles for Podxl in governing stress myelopoiesis. At steady state, Podxl expression among hematopoietic progenitor cells was low level but was induced by granulocyte colony-stimulating factor (G-CSF) in myeloid progenitors and by thrombopoietin in human stem cells...
June 2017: Experimental Hematology
https://www.readbyqxmd.com/read/28396819/rab11-fip1-phosphorylation-by-mark2-regulates-polarity-in-mdck-cells
#19
Rebecca McRae, Lynne A Lapierre, Elizabeth H Manning, James R Goldenring
MARK2/Par1b/EMK1, a serine/threonine kinase, is required for correct apical/basolateral membrane polarization in epithelial cells. However, the specific substrates mediating MARK2 action are less well understood. We have now found that MARK2 phosphorylates Rab11-FIP1B/C at serine 234 in a consensus site similar to that previously identified in Rab11-FIP2. In MDCK cells undergoing repolarization after a calcium switch, antibodies specific for pS234-Rab11-FIP1 or pS227-Rab11-FIP2 demonstrate that the spatial and temporal activation of Rab11-FIP1 phosphorylation is distinct from that for Rab11-FIP2...
2017: Cellular Logistics
https://www.readbyqxmd.com/read/28384052/pcmab-47-novel-antihuman-podocalyxin-monoclonal-antibody-for-immunohistochemistry
#20
Satoshi Ogasawara, Mika K Kaneko, Shinji Yamada, Ryusuke Honma, Takuro Nakamura, Noriko Saidoh, Miyuki Yanaka, Kanae Yoshida, Yuki Fujii, Yukinari Kato
Podocalyxin (PODXL) is a CD34-related sialomucin and a well-known marker of embryonic stem cells. PODXL is expressed in many types of tumors including colorectal cancers, breast cancers, and brain tumors. Overexpression of PODXL is an independent predictor of progression, metastasis, and poor outcome. PODXL is also expressed in many normal cells such as renal podocytes and endothelial cells (ECs). However, high-sensitive and high-specific anti-PODXL monoclonal antibodies (mAbs) have not been established. Herein, we immunized mice with recombinant human PODXL, which was produced using LN229 glioblastoma cells...
April 2017: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy
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