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https://www.readbyqxmd.com/read/27910957/mir-27a-attenuates-adipogenesis-and-promotes-osteogenesis-in-steroid-induced-rat-bmscs-by-targeting-ppar%C3%AE-and-grem1
#1
Chenxi Gu, Yan Xu, Shanfeng Zhang, Hongya Guan, Shi Song, Xiuli Wang, Yisheng Wang, Yuebai Li, Guoqiang Zhao
The imbalance between adipogenic and osteogenic differentiation in bone marrow mesenchymal stem cells (BMSCs) plays a significant role in the pathogenesis of steroid-induced osteonecrosis of the femoral head (ONFH). Several microRNAs (miRNAs) are involved in regulating adipogenesis and osteogenesis. In this study, we established a steroid-induced ONFH rat model to identify the potential relevant miRNAs. We identified 9 up-regulated and 28 down-regulated miRNAs in the ONFH rat model. Of these, miR-27a was down-regulated and negatively correlated with peroxisome proliferator-activated receptor gamma (PPARγ) and gremlin 1 (GREM1) expression...
December 2, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27910940/supportive-angiogenic-and-osteogenic-differentiation-of-mesenchymal-stromal-cells-and-endothelial-cells-in-monolayer-and-co-cultures
#2
Florian Böhrnsen, Henning Schliephake
Sites of implantation with compromised biology may be unable to achieve the required level of angiogenic and osteogenic regeneration. The specific function and contribution of different cell types to the formation of prevascularized, osteogenic networks in co-culture remains unclear. To determine how bone marrow-derived mesenchymal stromal cells (BMSCs) and endothelial cells (ECs) contribute to cellular proangiogenic differentiation, we analysed the differentiation of BMSCs and ECs in standardized monolayer, Transwell and co-cultures...
December 2, 2016: International Journal of Oral Science
https://www.readbyqxmd.com/read/27906439/in-vitro-neural-differentiation-of-bone-marrow-stromal-cells-induced-by-hepatocyte-growth-factor-and-glial-cell-derived-neurotrophic-factor
#3
Q Ma, M Cai, J-W Shang, J Yang, X-Y Gu, W-B Liu, Q Yang
OBJECTIVE: Bone marrow stromal cells (BMSCs) have great potential for cell-based transplantation therapy in treating neurological disease. However, the best combination of various trophic factors to produce full neural differentiation of BMSCs was still unclear. In our study, we aimed to investigate the neural differentiation capacity of rat BMSCs induced by growth factors including hepatocyte growth factor (HGF) and glial cell-derived neurotrophic factor (GDNF). MATERIALS AND METHODS:   Cell counting kit-8 (CCK-8) assay, BrdU cell proliferation assay and flow cytometry were implemented to evaluate whether GDNF and HGF had positive effects on the proliferation of BMSCs...
November 2016: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/27904711/naringin-promotes-osteogenic-differentiation-of-bone-marrow-stromal-cells-by-up-regulating-foxc2-expression-via-the-ihh-signaling-pathway
#4
Fei-Xiang Lin, Shi-Xin Du, De-Zhong Liu, Qin-Xiao Hu, Guo-Yong Yu, Chu-Cheng Wu, Gui-Zhou Zheng, Da Xie, Xue-Dong Li, Bo Chang
Naringin is an active compound extracted from Rhizoma Drynariae, and studies have revealed that naringin can promote proliferation and osteogenic differentiation of bone marrow stromal cells (BMSCs). In this study, we explored whether naringin could promote osteogenic differentiation of BMSCs by upregulating Foxc2 expression via the Indian hedgehog (IHH) signaling pathway. BMSCs were cultured in basal medium, basal medium with naringin, osteogenic induction medium, osteogenic induction medium with naringin and osteogenic induction medium with naringin in the presence of the IHH inhibitor cyclopamine (CPE)...
2016: American Journal of Translational Research
https://www.readbyqxmd.com/read/27904503/stem-cell-ophthalmology-treatment-study-scots-bone-marrow-derived-stem-cells-in-the-treatment-of-leber-s-hereditary-optic-neuropathy
#5
Jeffrey N Weiss, Steven Levy, Susan C Benes
The Stem Cell Ophthalmology Treatment Study (SCOTS) is currently the largest-scale stem cell ophthalmology trial registered at ClinicalTrials.gov (identifier: NCT01920867). SCOTS utilizes autologous bone marrow-derived stem cells (BMSCs) to treat optic nerve and retinal diseases. Treatment approaches include a combination of retrobulbar, subtenon, intravitreal, intra-optic nerve, subretinal, and intravenous injection of autologous BMSCs according to the nature of the disease, the degree of visual loss, and any risk factors related to the treatments...
October 2016: Neural Regeneration Research
https://www.readbyqxmd.com/read/27894122/b-cell-lymphoma-2-modified-bone-marrow-derived-mesenchymal-stem-cells-transplantation-for-the-treatment-of-diabetes-mellitus-induced-erectile-dysfunction-in-a-rat-model
#6
Xiang Sun, Long-Hua Luo, Liang Feng, Dong-Shui Li, Ke-Zhao Zhong
OBJECTIVE: The study aimed to explore the effects of B cell lymphoma-2 (Bcl-2)-modified bone marrow-derived mesenchymal stem cells (BMSCs) transplantation for the treatment of diabetes mellitus-induced erectile dysfunction (DMED) in a rat model. METHODS: The DMED rat model was successfully established. Thirty-six DMED rats were assigned into the Bcl-2-BMSCs, null-BMSCs, BMSCs and phosphate buffered saline (PBS) groups. Meanwhile, 9 normal rats injected with PBS were taken as the normal control group...
November 29, 2016: Urologia Internationalis
https://www.readbyqxmd.com/read/27891557/exogenous-expression-of-nt-3-and-trkc-genes-in-bone-marrow-stromal-cells-elevated-the-survival-rate-of-the-cells-in-the-course-of-neural-differentiation
#7
Houri Edalat, Zahra Hajebrahimi, Vahid Pirhajati, Mahmoud Tavallaei, Mansoureh Movahedin, Seyed Javad Mowla
Bone marrow stromal cells (BMSCs) are attractive cellular sources for cell therapy of many diseases, specifically neurodegenerative ones. The potential capability of BMSCs could be further augmented by enhancing their neuroprotective property, differentiation potential, and survival rate subsequent to transplantation. Therefore, a concurrent upregulation of neurotrophin-3 (NT-3) and its high affinity receptor, tyrosin kinase C (TrkC), was utilized in our study. BMSCs were cotransfected with pDsRed1-N1-NT-3 and pCMX-TrkC plasmids before induction of neural differentiation...
November 28, 2016: Cellular and Molecular Neurobiology
https://www.readbyqxmd.com/read/27890933/preclinical-activity-of-cpi-0610-a-novel-small-molecule-bromodomain-and-extra-terminal-protein-inhibitor-in-the-therapy-of-multiple-myeloma
#8
K T Siu, J Ramachandran, A J Yee, H Eda, L Santo, C Panaroni, J A Mertz, R J Sims, M R Cooper, N Raje
Inhibition of the bromodomain and extra-terminal (BET) proteins is a promising therapeutic strategy for various hematologic cancers. Previous studies suggest that BET inhibitors constrain tumor cell proliferation and survival mainly through suppression of MYC transcription and activity. However, suppression of the transcription of additional genes also contributes to the anti-tumor activity of BET inhibitors but is less well understood. Here we examined the therapeutic potential of CPI-0610, a potent BET inhibitor currently undergoing Phase I clinical testing, in multiple myeloma (MM)...
November 28, 2016: Leukemia: Official Journal of the Leukemia Society of America, Leukemia Research Fund, U.K
https://www.readbyqxmd.com/read/27890010/biological-function-and-mechanism-of-bone-marrow-mesenchymal-stem-cells-packed-poly-3-4-ethylenedioxythiophene-pedot-scaffolds-for-peripheral-nerve-injury-the-involvement-of-mir-21-notch-signaling-pathway
#9
Wenliang Wu, Shijun Zhang, Yunzhen Chen, Haichun Liu
Ununited peripheral nerves represent attractive site for connectivity with neuroprostheses because of their predictable internal topography allows for precise sorting of motor and sensory signals. Also transplantation of bone marrow mesenchymal stem cells (BMSCs) is increasingly recognized as an effective method of restore the peripheral nervous system injury due to its neuron-directed differentiation potential. This study was to evaluate the in vivo performance of BMSCs-packed Poly(3,4-ethylenedioxythiophene) (PEDOT) scaffolds across a critical nerve conduction gap and examine the potential mechanism by which BMSCs-packed PEDOT scaffolds mediates peripheral nerve regeneration in rat model of recurrent laryngeal nerve (RLN) deletion...
November 23, 2016: Current Neurovascular Research
https://www.readbyqxmd.com/read/27888418/suppression-of-mtor-signaling-pathway-promotes-bone-marrow-mesenchymal-stem-cells-differentiation-into-osteoblast-in-degenerative-scoliosis-in-vivo-and-in-vitro
#10
Yu Wang, Xiao-Dong Yi, Chun-De Li
To investigate the role of mTOR signaling pathway in bone marrow mesenchymal stem cells (BMSCs) differentiation into osteoblast in degenerative scoliosis (DS). The rat model of DS was established. Thirty-two Sprague-Dawley (SD) rats were selected and divided into the normal control group, the positive control group (normal rats injected with rapamycin), the negative control group (DS rats injected with PBS) and the experiment group (DS rats injected with rapamycin). H&E staining was performed to observe the osteogenesis of scoliosis...
November 25, 2016: Molecular Biology Reports
https://www.readbyqxmd.com/read/27885959/bone-marrow-stromal-cell-sheets-may-promote-axonal-regeneration-and-functional-recovery-with-suppression-of-glial-scar-formation-after-spinal-cord-transection-injury-in-rats
#11
Akinori Okuda, Noriko Horii-Hayashi, Takayo Sasagawa, Takamasa Shimizu, Hideki Shigematsu, Eiichiro Iwata, Yasuhiko Morimoto, Keisuke Masuda, Munehisa Koizumi, Manabu Akahane, Mayumi Nishi, Yasuhito Tanaka
OBJECTIVE Transplantation of bone marrow stromal cells (BMSCs) is a theoretical potential as a therapeutic strategy in the treatment of spinal cord injury (SCI). Although a scaffold is sometimes used for retaining transplanted cells in damaged tissue, it is also known to induce redundant immunoreactions during the degradation processes. In this study, the authors prepared cell sheets made of BMSCs, which are transplantable without a scaffold, and investigated their effects on axonal regeneration, glial scar formation, and functional recovery in a completely transected SCI model in rats...
November 25, 2016: Journal of Neurosurgery. Spine
https://www.readbyqxmd.com/read/27882117/glycitin-regulates-osteoblasts-through-tgf-%C3%AE-or-akt-signaling-pathways-in-bone-marrow-stem-cells
#12
Liyan Zhang, Jiying Chen, Wei Chai, Min Ni, Xin Sun, Dan Tian
The aim of the present study was to examine the effect of glycitin on the regulation of osteoblasts from bone marrow stem cells (BMSCs) through transforming growth factor (TGF)-β or protein kinase B (AKT) signaling pathways. BMSCs were extracted from New Zealand white rabbits and used to analyze the effect of glycitin on BMSCs. BMSCs were cleared using xylene and observed via light microscopy. BMSCs were subsequently induced with glycitin (0.01, 0.5, 1, 5 and 10 µM) for 7 days, and stained with Oil Red O...
November 2016: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/27878265/synergistic-effects-of-overexpression-of-bmp%C3%A2-2-and-tgf%C3%A2-%C3%AE-3-on-osteogenic-differentiation-of-bone-marrow-mesenchymal-stem-cells
#13
Yilin Wang, Tian He, Jie Liu, Hongzhi Liu, Lugang Zhou, Wei Hao, Yujie Sun, Xin Wang
Bone morphogenetic protein 2 (BMP-2) and transforming growth factor β (TGF-β) isoforms are important in advancing bone regeneration. The aim of the present study was to investigate the positive and reciprocal effect of TGF‑β3, one of the three TGF‑β isoforms, on BMP‑2 in promoting osteogenic differentiation. Exogenous BMP‑2 and TGF‑β3 genes were separately, and in combination, overexpressed in rabbit bone marrow‑derived mesenchymal stem cells (rBMSCs). Expression levels of BMP‑2 and TGF‑β3 were evaluated using reverse‑transcription‑polymerase chain reaction (RT‑PCR) and Western blotting assays...
November 22, 2016: Molecular Medicine Reports
https://www.readbyqxmd.com/read/27877865/three-dimensional-printed-macroporous-polylactic-acid-hydroxyapatite-composite-scaffolds-for-promoting-bone-formation-in-a-critical-size-rat-calvarial-defect-model
#14
Haifeng Zhang, Xiyuan Mao, Zijing Du, Wenbo Jiang, Xiuguo Han, Danyang Zhao, Dong Han, Qingfeng Li
We have explored the applicability of printed scaffold by comparing osteogenic ability and biodegradation property of three resorbable biomaterials. A polylactic acid/hydroxyapatite (PLA/HA) composite with a pore size of 500 μm and 60% porosity was fabricated by three-dimensional printing. Three-dimensional printed PLA/HA, β-tricalcium phosphate (β-TCP) and partially demineralized bone matrix (DBM) seeded with bone marrow stromal cells (BMSCs) were evaluated by cell adhesion, proliferation, alkaline phosphatase activity and osteogenic gene expression of osteopontin (OPN) and collagen type I (COL-1)...
2016: Science and Technology of Advanced Materials
https://www.readbyqxmd.com/read/27871114/bone-marrow-stromal-stem-cells-in-tissue-engineering-and-regenerative-medicine
#15
A Polymeri, W V Giannobile, D Kaigler
Bone marrow stromal stem cells (BMSCs) are adult multipotent cells, which have the potential to differentiate into cell types of mesodermal origin, namely osteocytes, adipocytes, and chondrocytes. Due to their accessibility and expansion potential, BMSCs have historically held therapeutic promise in tissue engineering and regenerative medicine applications. More recently, it has been demonstrated that not only can bone marrow stromal stem cells directly participate in tissue regeneration, but they also have the capacity to migrate to distant sites of tissue injury, where they can participate in tissue repair either directly through their differentiation or indirectly through paracrine mechanisms...
November 2016: Hormone and Metabolic Research, Hormon- und Stoffwechselforschung, Hormones et Métabolisme
https://www.readbyqxmd.com/read/27865878/bone-marrow-mesenchymal-stem-cells-decrease-chop-expression-and-neuronal-apoptosis-after-spinal-cord-injury
#16
Chuanlong Gu, Heyangzi Li, Chao Wang, Xinghui Song, Yuemin Ding, Mingzhi Zheng, Wei Liu, Yingying Chen, Xiaoming Zhang, Linlin Wang
Spinal cord injury (SCI) leads to irreversible neuronal loss and ultimately leads to paralysis. Bone marrow derived mesenchymal stem cells (BMSCs) have been demonstrated to be an effective approach to treat SCI. The present study was designed to investigate the role of BMSCs in rats with spinal cord injury and in oxygen-glucose deprivation (OGD) treated motor neurons. The results demonstrated that BMSCs could improve locomotor function and decrease expression of pro-apoptotic transcription factor C/EBP homologous protein (CHOP) and apoptosis after SCI...
November 16, 2016: Neuroscience Letters
https://www.readbyqxmd.com/read/27864663/mesenchymal-stem-cells-attenuate-hydrogen-peroxide-induced-oxidative-stress-and-enhance-neuroprotective-effects-in-retinal-ganglion-cells
#17
Yi Cui, Nuo Xu, Wei Xu, Guoxing Xu
The apoptosis of retinal ganglion cells leads to visual impairment and blindness in ocular neurodegenerative diseases, especially in diabetic retinopathy (DR). Mounting evidence suggests that oxidative stress contributes to the pathogenesis of DR. In the present study, we investigated whether bone mesenchymal stem cells (BMSCs) have protective ability to relieve hydrogen peroxide (H2O2)-induced injury on retinal ganglion cells in vitro. An immortalized retinal ganglion cells, RGC-5 cells, were exposed to an indicated concentration of H2O2 for 24 h...
November 18, 2016: In Vitro Cellular & Developmental Biology. Animal
https://www.readbyqxmd.com/read/27862699/microrna-503-5p-inhibits-stretch-induced-osteogenic-differentiation-and-bone-formation
#18
Lu Liu, Mengjun Liu, Rongrong Li, Hong Liu, Liling Du, Hong Chen, Yan Zhang, Shijie Zhang, Dongxu Liu
Cyclical stretch-induced bone formation during orthodontic treatment is a complex biological process modulated by various factors including miRNAs and their targeted-gene network. However, the miRNA expression profile and their roles in osteogenic differentiation of bone mesenchymal stem cells (BMSCs) exposed to mechanical stretch remains unclear. Here, we use the miRNA microarray assay to screen for mechano-sensitive miRNAs during stretch-induced osteogenic differentiation of BMSCs and identified that nine miRNAs were differentially expressed between stretched and control BMSCs...
November 11, 2016: Cell Biology International
https://www.readbyqxmd.com/read/27859871/antheraea-pernyi-silk-sericin-mediating-biomimetic-nucleation-and-growth-of-hydroxylapatite-crystals-promoting-bone-matrix-formation
#19
Zhuang Jiayao, Zhou Guanshan, Zhang Jinchi, Chen Yuyin, Zhu Yongqiang
Bone biomineralization is well-regulated processes mediated by extracellular matrix proteins. The materials that can direct nucleation of hydroxylapatite (HAp) crystals and assembly of well-structured material-minerals complex are the key to mimicking the natural mineralization. This study used sericin from Antheraea pernyi (A.pernyi), non-mulberry silkworm cocoon as template to mediate nucleation of HAp crystals. Here we find out that AS (Antheraea pernyi sericin) can nucleate the formation HAp crystals in simulated body fluid verified by XRD and FTIR observations...
November 18, 2016: Microscopy Research and Technique
https://www.readbyqxmd.com/read/27859595/recipient-bone-marrow-derived-stromal-cells-prolong-graft-survival-in-a-rat-hind-limb-allotransplantation-model
#20
Ryosuke Ikeguchi, Ryosuke Kakinoki, Souichi Ohta, Hiroki Oda, Hirofumi Yurie, Yukitoshi Kaizawa, Hiroto Mitsui, Tomoki Aoyama, Junya Toguchida, Shuichi Matsuda
BACKGROUND: Recent studies have indicated that bone marrow-derived stromal cells (BMSCs) have immunomodulatory properties that suppress the T cell responses that cause graft rejection. The purpose of this study is to evaluate the effect of recipient BMSCs intravenous infusion for immunomodulation in a rat vascularized composite allotransplantation model. METHODS: A total of nine Wistar (WIS) rats and thirty Lewis (LEW) rats were used. BMSCs were harvested from three LEW rats...
November 17, 2016: Microsurgery
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