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https://www.readbyqxmd.com/read/28911171/sirtuin-3-promotes-adipogenesis-osteoclastogenesis-and-bone-loss-in-aging-male-mice
#1
Linh Ho, Liping Wang, Theresa M Roth, Yong Pan, Eric M Verdin, Edward C Hsiao, Robert A Nissenson
Sirtuin-3 (Sirt3) is an essential metabolic regulatory enzyme that plays an important role in mitochondrial metabolism, but its role in bone marrow and skeletal homeostasis remains largely unknown. In this study, we hypothesize that increased expression of Sirt3 plays a role in skeletal aging. Using mice that overexpress Sirt3 [i.e., Sirt3 transgenic (Sirt3Tg)], we show that Sirt3 is a positive regulator of adipogenesis and osteoclastogenesis and a negative regulator of skeletal homeostasis. Sirt3Tg mice exhibited more adipocytes in the tibia compared with control mice...
September 1, 2017: Endocrinology
https://www.readbyqxmd.com/read/28903624/repair-of-calvarial-bone-defect-using-jarid1a-knockdown-bone-mesenchymal-stem-cells-in-rats
#2
Yuan Deng, Tao Guo, Jipeng Li, Li Guo, Ping Gu, Xianqun Fan
Histone methylation is regarded as an important epigenetic event during stem cell differentiation. Jumonji AT-rich interactive domain 1A (Jarid1a) is a histone demethylase that specifically catalyzes demethylation of dimethyl or trimethyl histone H3K4me3, which is normally associated with transcriptionally active genes. Runt-related transcription factor 2 (Runx2) has been identified as a key transcription factor in the early stage of osteogenesis. A better understanding of this epigenetic mechanism that governs osteogenic differentiation of bone mesenchymal stem cells (BMSCs) can provide new insights into BMSCs based bone tissue engineering...
September 13, 2017: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/28901483/moderate-hypothermia-induces-protein-sumoylation-in-bone-marrow-stromal-cells-and-enhances-their-tolerance-to-hypoxia
#3
Wenbo Ren, Xiaofang Ma, Xiaozhi Liu, Yanxia Li, Zhongmin Jiang, Yujun Zhao, Chen Li, Xin Li
Acute cerebral infarction can progress rapidly, and there are limited specific and effective treatments. Small ubiquitin‑like modifiers (SUMOs) provide an important post‑translational modification of proteins. Following cerebral infarction, multiple proteins can combine with SUMOs to protect nerve cells. Furthermore, moderate hypothermia (core body temperature, 33‑34˚C) can increase the level of SUMOylation on multiple proteins. In the present study, it was examined whether moderate hypothermia increases the survival rate of bone marrow stromal stem cells (BMSCs) implanted in the cerebral ischemic penumbra via SUMOylation of multiple proteins...
September 5, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28901383/endothelial-progenitor-cell%C3%A2-derived-extracellular-vesicle%C3%A2-meditated-cell%C3%A2-to%C3%A2-cell-communication-regulates-the-proliferation-and-osteoblastic-differentiation-of-bone-mesenchymal-stromal-cells
#4
Yunhao Qin, Changqing Zhang
Bone tissue engineering is a promising treatment strategy to increase bone regeneration. Endothelial progenitor cells (EPCs) and bone marrow stromal cells (BMSCs) are commonly used to promote vessel formation and osteoblastic differentiation in tissue engineering. Previous studies have demonstrated that EPCs regulate both proliferation and differentiation of BMSCs. However, the underlying mechanism remains unclear. Understanding this mechanism is critical to developing more effective treatments. The role of extracellular vesicles in cell‑to‑cell communication has attracted substantial attention...
August 31, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28900122/projection-stereolithographic-fabrication-of-bmp-2-gene-activated-matrix-for-bone-tissue-engineering
#5
Hang Lin, Ying Tang, Thomas P Lozito, Nicholas Oyster, Robert B Kang, Madalyn R Fritch, Bing Wang, Rocky S Tuan
Currently, sustained in vivo delivery of active bone morphogenetic protein-2 (BMP-2) protein to responsive target cells, such as bone marrow-derived mesenchymal stem cells (BMSCs), remains challenging. Ex vivo gene transfer method, while efficient, requires additional operation for cell culture and therefore, is not compatible with point-of-care treatment. In this study, two lentiviral gene constructs - (1) Lv-BMP/GFP, containing human BMP-2 and green fluorescent protein (GFP) gene (BMP group); or (2) Lv-GFP, containing GFP gene (GFP group) - were incorporated with human BMSCs into a solution of photocrosslinkable gelatin, which was then subjected to visible light-based projection stereolithographic printing to form a scaffold with desired architectures...
September 12, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28890164/directed-differentiation-of-human-bone-marrow-stromal-cells-to-fate-committed-schwann-cells
#6
Sa Cai, Yat-Ping Tsui, Kin-Wai Tam, Graham Ka-Hon Shea, Richard Shek-Kwan Chang, Qiang Ao, Daisy Kwok-Yan Shum, Ying-Shing Chan
Our ultimate goal of in vitro derivation of Schwann cells (SCs) from adult bone marrow stromal cells (BMSCs) is such that they may be used autologously to assist post-traumatic nerve regeneration. Existing protocols for derivation of SC-like cells from BMSCs fall short in the stability of the acquired phenotype and the functional capacity to myelinate axons. Our experiments indicated that neuro-ectodermal progenitor cells among the human hBMSCs could be selectively expanded and then induced to differentiate into SC-like cells...
August 29, 2017: Stem Cell Reports
https://www.readbyqxmd.com/read/28888020/biocompatible-%C3%AE-srhpo4-clusters-with-dandelion-like-structure-as-an-alternative-drug-carrier
#7
Haishan Shi, Tingting Wu, Jing Zhang, Xiaoling Ye, Shenghui Zeng, Xu Liu, Tao Yu, Jiandong Ye, Changren Zhou
Recent researches about calcium phosphate (CaP) biomaterials used as drug delivery systems are focusing on the better understanding of the microenvironment around the implant-host tissue interface, with the aim to provide a bone response in pathological ones. Towards the improvement of the osteogenic potential of CaP drug carriers, dandelion-like β-SrHPO4 clusters (Φ10-20μm) has been prepared by a homogeneous precipitation method under the hydrolysis of carbamide. Adhesion, spreading, proliferation, osteogenic differentiation and mRNA expression of bone mesenchymal stem cells (BMSCs) mediated by β-SrHPO4 clusters were investigated...
December 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28887585/biomechanical-properties-and-cellular-biocompatibility-of-3d-printed-tracheal-graft
#8
Yibo Shan, Yao Wang, Jianfeng Li, Hongcan Shi, Yiwei Fan, Junfeng Yang, Weidong Ren, Xi Yu
The goals of our study were to evaluate the biomechanical properties and cellular biocompatibility of 3D printed tracheal graft fabricated by polycaprolactone (PCL). Compared with native tracheal patch, there was a significant increase in maximum stress and elastic modulus for 3DP tracheal graft (p < 0.05). BMSCs were co-cultured under four different conditions to investigate cytotoxicity of the graft: (1) co-cultured with normal culture medium, as blank control; (2) co-cultured with perfluoropropylene, as negative control; (3) co-cultured with 3DP tracheal graft; and (4) co-cultured with polyvinyl chloride, as positive control...
September 8, 2017: Bioprocess and Biosystems Engineering
https://www.readbyqxmd.com/read/28886699/research-on-advanced-intervention-using-novel-bone-marrow-stem-cell-rainbow-a-study-protocol-for-a-phase-i-open-label-uncontrolled-dose-response-trial-of-autologous-bone-marrow-stromal-cell-transplantation-in-patients-with-acute-ischemic-stroke
#9
Hideo Shichinohe, Masahito Kawabori, Hiroaki Iijima, Tuyoshi Teramoto, Takeo Abumiya, Naoki Nakayama, Ken Kazumata, Shunsuke Terasaka, Teruyo Arato, Kiyohiro Houkin
BACKGROUND: Stroke is a leading cause of death and disability, and despite intensive research, few treatment options exist. However, a recent breakthrough in cell therapy is expected to reverse the neurological sequelae of stroke. Although some pioneer studies on the use of cell therapy for treating stroke have been reported, certain problems remain unsolved. Recent studies have demonstrated that bone marrow stromal cells (BMSCs) have therapeutic potential against stroke. We investigated the use of autologous BMSC transplantation as a next-generation cell therapy for treating stroke...
September 8, 2017: BMC Neurology
https://www.readbyqxmd.com/read/28884494/multilayered-pore-closed-plga-microsphere-delivering-ogp-and-bmp-2-in-sequential-release-patterns-for-the-facilitation-of-bmscs-osteogenic-differentiation
#10
Bing-Jun Zhang, Zhi-Wei Han, Ke Duan, Yan-Dong Mu, Jie Weng
Bone tissue regeneration may be more effectively administrated by controlled release of multiple biofactors, given that bone healing comprises a cascade of biological events controlled by numerous cytokines and growth factors (GFs). Here, we propose a novel microcarrier with the capability to sequentially deliver dual biofactors for better controlling the bone regeneration process. Firstly, osteogenic growth peptide (OGP) was incorporated in porous poly (lactic-co-glycolic) acid (PLGA) microspheres by a simple solution dipping method and subsequent pore-closing treatment...
September 7, 2017: Journal of Biomedical Materials Research. Part A
https://www.readbyqxmd.com/read/28881756/mm-bmscs-induce-na%C3%A3-ve-cd4-t-lymphocytes-dysfunction-through-fibroblast-activation-protein-%C3%AE
#11
Xiaofei Wu, Yadan Wang, Jian Xu, Ting Luo, Jun Deng, Yu Hu
BACKGROUND: The tumor microenvironment plays a major role in multiple myelomas (MM). MM-BMSCs (bone marrow mesenchymal stromal cells) can support tumor growth and immune surveillance escape. On the other hand, fibroblast activation protein α, expressed by cancer stroma cells including BMSCs, has been shown to potentiate epithelial cancers growth and immune suppression. RESULTS: MM-BMSC inhibited proliferation of T cells (P = 0.0138), promoted senescence of T cells (P < 0...
August 8, 2017: Oncotarget
https://www.readbyqxmd.com/read/28880271/matrine-derivate-masm-uncovers-a-novel-function-for-ribosomal-protein-s5-in-osteoclastogenesis-and-postmenopausal-osteoporosis
#12
Xiao Chen, Xin Zhi, Liehu Cao, Weizong Weng, Panpan Pan, Honggang Hu, Chao Liu, Qingjie Zhao, Qirong Zhou, Jin Cui, Jiacan Su
Postmenopausal osteoporosis (POMP) is a public health problem characterized by decreased bone density and increased fracture risk. Over-activated osteoclastogenesis plays a vital role in POMP. Here we developed a novel bioactive compound MASM (M19) based on sophocarpine. Although it showed no significant effects on osteogenesis and adipogenesis for bone marrow-derived mesenchymal stem cells (BMSCs) in vitro, it could significantly inhibit RANKL/M-CSF induced osteoclastogenesis through suppressing NF-κB, MAPKs and PI3K/Akt pathways in vitro and ameliorate bone loss in ovariectomized mice in vivo...
September 7, 2017: Cell Death & Disease
https://www.readbyqxmd.com/read/28877384/exosomes-secreted-from-mutant-hif-1%C3%AE-modified-bone-marrow-derived-mesenchymal-stem-cells-attenuate-early-steroid-induced-avascular-necrosis-of-femoral-head-in-rabbit
#13
Haile Li, Danping Liu, Chen Li, Shanjian Zhou, Dachuan Tian, Dawei Xiao, Huan Zhang, Feng Gao, Jianhua Huang
Mesenchymal stem cells (MSCs)-derived exosomes exhibit protective effects on damaged or diseased tissues. Hypoxia-inducible factor 1α (HIF-1α) plays a critical role in bone development. However, HIF-1α is easily biodegradable under normoxic conditions. The bone-marrow-derived mesenchymal stem cells (BMSCs) were transfected with adenovirus carrying triple point-mutations (amino acids 402, 564, and 803) in the HIF-1α coding sequence (CDS). The mutant HIF-1α can efficiently express functional proteins under normoxic conditions...
September 6, 2017: Cell Biology International
https://www.readbyqxmd.com/read/28872083/-repair-of-articular-cartilage-defects-by-autologous-bone-mesenchymal-stem-cells-and-allogeneic-costal-chondrocytes-in-the-knee-of-wuzhishan-miniature-pigs
#14
Cheng Yang, Jiangdong Ni, Shou Zhang, Zhongcheng Fan
To investigate the feasibility of construction of tissue engineered cartilage by co-culture of bone marrow mesenchymal stem cells (BMSCs) and costal chondrocytes (CCs), and to provide theoretical basis and experimental basis for clinical repair of articular cartilage defects by Wuzhishan miniature pig knee cartilage defects with co-cultured cells.
 Methods: Density gradient centrifugation method was used to isolate BMSCs from Wuzhishan miniature pig. The double enzyme digestion method was used to isolate CCs...
August 28, 2017: Zhong Nan da Xue Xue Bao. Yi Xue Ban, Journal of Central South University. Medical Sciences
https://www.readbyqxmd.com/read/28871264/neuroinflammation-bone-marrow-stem-cells-and-chronic-pain
#15
REVIEW
Yul Huh, Ru-Rong Ji, Gang Chen
Current treatments for chronic pain, such as inflammatory pain, neuropathic pain, and cancer pain are insufficient and cause severe side effects. Mounting evidence suggests that neuroinflammation in the peripheral and central nervous system (PNS and CNS) plays a pivotal role in the genesis and maintenance of chronic pain. Characteristic features of neuroinflammation in chronic pain conditions include infiltration of immune cells into the PNS [e.g., the sciatic nerve and dorsal root ganglion (DRG)], activation of glial cells such as microglia and astrocytes in the CNS (spinal cord and brain), and production and secretion of pro-inflammatory cytokines and chemokines [TNF, interleukin (IL)-1β, IL-6, CCL2, and CXCL1]...
2017: Frontiers in Immunology
https://www.readbyqxmd.com/read/28867364/cell-based-and-pharmacological-neurorestorative-therapies-for-ischemic-stroke
#16
REVIEW
Poornima Venkat, Yi Shen, Michael Chopp, Jieli Chen
Ischemic stroke remains one of most common causes of death and disability worldwide. Stroke triggers a cascade of events leading to rapid neuronal damage and death. Neuroprotective agents that showed promise in preclinical experiments have failed to translate to the clinic. Even after decades of research, tPA remains the only FDA approved drug for stroke treatment. However, tPA is effective when administered 3-4.5 h after stroke onset and the vast majority of stroke patients do not receive tPA therapy. Therefore, there is a pressing need for novel therapies for ischemic stroke...
September 1, 2017: Neuropharmacology
https://www.readbyqxmd.com/read/28861713/lncrna-zeb1-as1-contributes-to-stat3-activation-by-associating-with-il-11-in-b-lymphoblastic-leukemia
#17
Qing Wang, Xiaoli Du, Min Yang, Shishan Xiao, Jun Cao, Jun Song, Linhui Wang
OBJECTIVE: To investigate the role of lncRNA ZEB1-AS1 in B-lineage acute lymphoblastic leukemia (B-ALL). RESULTS: ZEB1-AS1 levels were aberrantly up-regulated in B-ALL. All correlated with STAT3 activation and IL-11 production. Moreover, a high level of ZEB1-AS1 predicted poor prognosis of B-ALL patients. Mechanistically, ZEB1-AS1 could bind to IL-11 and promote IL-11 stability. Down-regulation of ZEB1-AS1 decreased IL-11 production of human bone marrow stromal cells (BMSCs), which led to suppressed proliferation and inhibited IL-11/STAT3 pathway in BALL-1 cells...
August 31, 2017: Biotechnology Letters
https://www.readbyqxmd.com/read/28861163/image-guided-stem-cells-with-functionalized-self-assembling-peptide-nanofibers-for-treatment-of-acute-myocardial-infarction-in-a-mouse-model
#18
Xiao Li, Ying-Ying Chen, Xiu-Mei Wang, Kai Gao, Yun-Zhou Gao, Jian Cao, Zhuo-Li Zhang, Jing Lei, Zheng-Yu Jin, Yi-Ning Wang
AIM: To investigate the survival of bone marrow mesenchymal stem cells (BMSCs) and the therapeutic effect for acute myocardial infarction (AMI) after co-transplantation with the functionalized self-assembling peptide nanofiber RAD/PRG or RAD/KLT. METHODS: AMI of balb/c mice was induced. Mice were randomly divided into four groups, and received treatment of phosphate buffered saline (PBS) (Group A), GFP/Fluc-BMSCs (Group B), GFP/Fluc-BMSCs + RAD/PRG (Group C), and GFP/Fluc-BMSCs + RAD/KLT (Group D), respectively...
2017: American Journal of Translational Research
https://www.readbyqxmd.com/read/28860501/in-vivo-immune-interactions-of-multipotent-stromal-cells-underlie-their-long-lasting-pain-relieving-effect
#19
Wei Guo, Satoshi Imai, Jia-Le Yang, Shiping Zou, Mineo Watanabe, Yu-Xia Chu, Zaid Mohammad, Huakun Xu, Kamal D Moudgil, Feng Wei, Ronald Dubner, Ke Ren
Systemic infusion of bone marrow stromal cells (BMSCs), a major type of multipotent stromal cells, produces pain relief (antihyperalgesia) that lasts for months. However, studies have shown that the majority of BMSCs are trapped in the lungs immediately after intravenous infusion and their survival time in the host is inconsistent with their lengthy antihyperalgesia. Here we show that long-lasting antihyperalgesia produced by BMSCs required their chemotactic factors such as CCL4 and CCR2, the integrations with the monocytes/macrophages population, and BMSC-induced monocyte CXCL1...
August 31, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28859160/igfbp2-enhances-adipogenic-differentiation-potentials-of-mesenchymal-stem-cells-from-wharton-s-jelly-of-the-umbilical-cord-via-jnk-and-akt-signaling-pathways
#20
Yuejun Wang, Yunsong Liu, Zhipeng Fan, Dayong Liu, Fu Wang, Yongsheng Zhou
Mesenchymal stem cell (MSC)-mediated tissue engineering represents a promising strategy to address adipose tissue defects. MSCs derived from Wharton's jelly of the umbilical cord (WJCMSCs) may serve as an ideal source for adipose tissue engineering due to their abundance, safety profile, and accessibility. How to activate the directed differentiation potentials of WJCMSCs is the core point for their clinical applications. A thorough investigation of mechanisms involved in WJCMSC adipogenic differentiation is necessary to support their application in adipose tissue engineering and address shortcomings...
2017: PloS One
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