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https://www.readbyqxmd.com/read/28642327/declining-histone-acetyltransferase-gcn5-represses-bmsc-mediated-angiogenesis-during-osteoporosis
#1
Huan Jing, Li Liao, Xiaoxia Su, Yi Shuai, Xinjing Zhang, Zhihong Deng, Yan Jin
Angiogenesis is disrupted in age-related and postmenopausal osteoporosis. However, the mechanisms of the disorder remain elusive. We confirmed in this study that, in accordance with the decrease of H-type vessels, the proangiogenic potential of bone marrow-derived mesenchymal stem cells (BMSCs) declined during osteoporosis. Screening of the histone acetyltransferase family revealed that GCN5 decreased in BMSCs derived from osteoporotic femur. Further analysis identified that GCN5 plays important roles in regulating the proangiogenic potential of BMSCs...
June 22, 2017: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
https://www.readbyqxmd.com/read/28636974/sema3a-partially-reverses-vegf-effects-through-binding-to-neuropilin-1
#2
Bruna Palodetto, Adriana da Silva Santos Duarte, Matheus Rodrigues Lopes, Flavia Adolfo Corrocher, Fernanda Marconi Roversi, Fernanda Soares Niemann, Karla Priscila Vieira Ferro, Ana Leda Figueiredo Longhini, Paula Melo Campos, Patricia Favaro, Sara Teresinha Olalla Saad
Cross-talk between hematopoietic stem cells (HSCs) and bone marrow stromal cells (BMSCs) is essential for HSCs regulation and leukemogenesis. Studying bone marrow of myelodysplasia patients, a pre-leukemic condition, we found mRNA overexpression of vascular endothelial growth factor A (VEGFA) in CD34(+) HSCs and semaphorin 3A (SEMA3A) in BMSCs. To better understand the role of VEGFA and SEMA3A in leukemogenesis, we recruited 30 myelodysplastic syndrome (MDS) patients, 29 acute myeloid leukemia (6 secondary to MDS) patients and 12 controls...
June 3, 2017: Stem Cell Research
https://www.readbyqxmd.com/read/28635301/stem-cell-mediated-paracrine-signaling-alters-fibroplasia-in-human-vocal-fold-fibroblasts-in-vitro
#3
Nao Hiwatashi, Renjie Bing, Iv Kraja, Ryan C Branski
OBJECTIVES: Interactions between mesenchymal stem cells (MSCs) and native vocal fold fibroblasts (VFFs) have not been described in spite of promising preliminary data regarding the effects of MSCs on vocal fold repair in vivo. The current study employed a conditioned media (CM) model to investigate the paracrine effects of bone marrow-derived mesenchymal stem cells (BMSCs) on VFFs. METHODS: Human VFFs were treated with transforming growth factor-β1 (TGF-β1; 10 ng/mL), CM from human BMSCs following 48 hours of TGF-β1 stimulation, or CM+TGF-β1...
June 1, 2017: Annals of Otology, Rhinology, and Laryngology
https://www.readbyqxmd.com/read/28631613/repairing-a-bone-defect-with-a-three-dimensional-cellular-construct-composed-of-a-multi-layered-cell-sheet-on-electrospun-mesh
#4
Zhiwei Ren, Shiqing Ma, Le Jin, Zihao Liu, Deping Liu, Xu Zhang, Qing Cai, Xiaoping Yang
In addition to providing maneuverability, electrospun nanofibrous meshes can make excellent supports for constructing flexible cell sheets to regulate cell behavior by nanofiber features. With the target of bone regeneration, herein composite nanofibers with two different fiber arrangements (nestlike, random) were electrospun from a blend solution containing poly(l-lactide) (PLLA) and gelatin (1:1 in weight ratio). Unlike the non-woven morphology in a random nanofibrous mesh, PLLA/gelatin composite nanofibers in the nestlike nanofibrous mesh displayed both non-woven and parallel morphologies...
June 20, 2017: Biofabrication
https://www.readbyqxmd.com/read/28629266/polygonatum-sibiricum-polysaccharide-promotes-osteoblastic-differentiation-through-the-erk-gsk-3%C3%AE-%C3%AE-catenin-signaling-pathway-in-vitro
#5
Xiaoming Peng, Jichen He, Jinmin Zhao, Yunle Wu, Xiongzhi Shi, Li Du, Mengni Nong, Shaohui Zong, Gao-Feng Zeng
Natural compound Polygonatum sibiricum polysaccharide (PSP) has a variety of biological actions such as reducing blood fat, antitumor, antioxidant activities, and enhancing immune function. In our previous study has revealed that PSP can promote osteoblastic differentiation in bone mesenchymal stem cells (BMSCs) by increasing nuclear accumulation of β-catenin. This study was designed to investigate that PSP can upregulate nuclear β-catenin, which was prevented by inhibiting glycogen synthase kinase 3β (GSK-3β) activity, by effectively activating Wnt signaling independent of low-density lipoprotein receptor-related protein (LRP5) ...
June 19, 2017: Rejuvenation Research
https://www.readbyqxmd.com/read/28624869/incorporation-of-cerium-oxide-into-hydroxyapatite-coating-protects-bone-marrow-stromal-cells-against-h2o2-induced-inhibition-of-osteogenic-differentiation
#6
Kai Li, Qingyi Shen, Youtao Xie, Mingyu You, Liping Huang, Xuebin Zheng
Oxidative stress exerts a key influence in osteoporosis in part by inhibiting osteogenic differentiation of bone marrow stromal cells (BMSCs). With their unique antioxidant properties and reported biocompatibility, cerium oxide (CeO2) ceramics exhibit promising potential for the treatment of osteoporosis resulting from oxidative stress. In this study, protective effects of CeO2-incorporated hydroxyapatite coatings (HA-10Ce and HA-30Ce) on the viability and osteogenic differentiation of H2O2-treated BMSCs were examined...
June 17, 2017: Biological Trace Element Research
https://www.readbyqxmd.com/read/28624824/effect-of-tnf-%C3%AE-inhibition-on-bone-marrow-derived-mesenchymal-stem-cells-in-neurological-function-recovery-after-spinal-cord-injury-via-the-wnt-signaling-pathway-in-a-rat-model
#7
Ren-Jun Peng, Bing Jiang, Xi-Ping Ding, He Huang, Yi-Wei Liao, Gang Peng, Quan Cheng, Jian Xi
AIM: The present study aimed to examine the effect of tumor necrosis factor-α (TNF-α) inhibition on bone marrow-derived mesenchymal stem cells (BMSCs) in neurological function recovery after spinal cord injury (SCI) via the Wnt signaling pathway in a rat model. METHODS: The rat model of SCI was established using Allen's method. Seventy-two adult male Sprague Dawley (SD) rats were randomly assigned into 4 groups (18 rats in each group): the sham control group, saline control group, BMSCs group (injection with BMSCs at the injured site) and BMSCs + TNF-α group (injection with BMSCs under TNF-α treatment at the injured site)...
June 15, 2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/28623357/adiponectin-regulates-bmsc-osteogenic-differentiation-and-osteogenesis-through-the-wnt-%C3%AE-catenin-pathway
#8
Yiyao Wang, Xiaohui Zhang, Jun Shao, Hanghang Liu, Xian Liu, En Luo
Recent studies have demonstrated the stimulatory effects of adiponectin on bone formation, but the mechanism underlying these effects remains unclear. The Wnt/β-catenin pathway, one of the most important pathways in osteogenesis, has rarely been associated with the osteogenic effects of adiponectin in previous studies. The present study was designed to investigate the effects of adiponectin on bone mesenchymal stem cell (BMSC) osteogenic differentiation and bone formation through the Wnt/β-catenin pathway...
June 16, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28617560/influence-of-vegf-bmp-2-on-the-proliferation-and-osteogenetic-differentiation-of-rat-bone-mesenchymal-stem-cells-on-plga-gelatin-composite-scaffold
#9
G An, W-B Zhang, D-K Ma, B Lu, G-J Wei, Y Guang, C-H Ru, Y-S Wang
OBJECTIVE: To investigate the influence of VEGF/BMP-2 on the proliferation and osteogenic differentiation of rat bone mesenchymal stem cells BMSCs) on PLGA/gelatin composite scaffold. MATERIALS AND METHODS: Randomly-oriented nanofibers with different ratios of Poly Lactic-co-Glycolic Acid (PLGA)/gelatin were produced through electrospinning. The mixture of nanofibers and BMSCs was pipetted onto the surface of the scaffolds, and BMSCs/PLGA/gelatin composite was obtained...
May 2017: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/28616703/preparation-of-a-biphase-composite-scaffold-and-its-application-in-tissue-engineering-for-femoral-osteochondral-defects-in-rabbits
#10
Shi-Qiang Ruan, Ling Yan, Jiang Deng, Wen-Liang Huang, Dian-Ming Jiang
PURPOSE: Three-dimensional bioactive scaffolds are useful tools for stem cell implant in tissue-engineering. For chondral and subchondral repair, the chondroinductive and osteoinductive property of a scaffold is a major challenge. The scaffolds that aim to osteogenic differentiation have been well studied. However, cartilage cells can hardly be induced for osteogenesis, and monophase scaffolds cannot ideally repair both cartilage and subchondral defects at the same time. METHODS: We developed a novel biphase composite scaffold and observe its application osteochondral defects...
June 14, 2017: International Orthopaedics
https://www.readbyqxmd.com/read/28608614/solanum-muricatum-promotes-osteogenic-differentiation-of-rat-bone-marrow-stromal-cells
#11
Nan Wang, Feng Wang, Youshui Gao, Zubin Zhou, Wei Liu, Chenhao Pan, Peipei Yin, Mingjie Tang, Xiaowei Yu
Solanum muricatum (SM), also known as pepino, is known for its antioxidative and anti-inflammatory effects. The aim of this study was to evaluate the effects of SM extract in promoting osteogenic differentiation and regulating the Wnt and bone morphogenetic protein (BMP) signaling pathways. Ingredients of pepino were extracted and identified. SM extracts were used to treat rat bone marrow stromal cells (BMSCs), followed by evaluating alkaline phosphatase activities and mineralization levels. The mRNA levels of osteogenic biomarkers, including OPN and Collagen I, were also evaluated with real-time polymerase chain reaction...
June 13, 2017: Journal of Food Science
https://www.readbyqxmd.com/read/28602124/composite-scaffolds-of-mineralized-natural-extracellular-matrix-on-true-bone-ceramic-induce-bone-regeneration-via-smad1-5-8-and-erk1-2-pathways
#12
Tingfang Sun, Sheng Yao, Man Liu, Yushi Yang, Yanhui Ji, Wei Cui, Yanzhen Qu, Xiao-Dong Guo
A stem cell-derived mineralized extracellular matrix (ECM) may be a good strategy to endow scaffolds with a bone microenvironment, thus inducing bone regeneration. However, it also faces some challenges, such as limited cell number, additional wound for autologous cell isolation, and time consumption of cell expansion. In this study, we designed a novel tissue-derived ECM scaffold fabricated by depositing porcine small intestinal submucosa (SIS) ECM on true bone ceramic (TBC), which was followed by mineralization treatment (mSIS/TBC)...
June 10, 2017: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/28596582/cell-sheets-of-co-cultured-endothelial-progenitor-cells-and-mesenchymal-stromal-cells-promote-osseointegration-in-irradiated-rat-bone
#13
Huan Liu, Wei Zhou, Nan Ren, Zhihong Feng, Yan Dong, Shizhu Bai, Yang Jiao, Zhongshan Wang, Yimin Zhao
Irradiated bone has a greater risk of implant failure than nonirradiated bone. The purpose of this study was to investigate the influence of cell sheets composed of co-cultured bone marrow mesenchymal stromal cells (BMSCs) and endothelial progenitor cells (EPCs) on implant osseointegration in irradiated bone. Cell sheets (EPCs, BMSCs or co-cultured EPCs and BMSCs) were wrapped around titanium implants to make cell sheet-implant complexes. The co-cultured group showed the highest osteogenic differentiation potential in vitro, as indicated by the extracellular matrix mineralization and the expression of osteogenesis related genes at both mRNA and protein levels...
June 8, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28587355/morphological-and-functional-changes-in-bone-marrow-mesenchymal-stem-cells-in-rats-with-heart-failure
#14
Xiuli Wang, Chunmei Li, Haibin Gong
The changes in bone marrow-derived mesenchymal stem cells (BMSCs), in terms of cell morphology and protein expression in rats with heart failure, were studied. Pressure overload chronic heart failure rat model was induced with partial constriction of the abdominal aorta. BMSCs from the model and the sham operation groups were isolated and cultured (cell density, 10(8) cells/l), and supernatant was collected after 72 h. Enzyme-linked immunosorbent assay was used to measure HGF, IGF-1, PDGF, SCF, FGF and VEGF levels in the supernatant...
June 2017: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/28587336/effects-of-polyvinylpyrrolidone-iodine-on-tendon-bone-healing-in-a-rabbit-extra-articular-model
#15
Peng Zhang, Yunlong Zhi, Hongwei Fang, Ziying Wu, Tianwu Chen, Jia Jiang, Shiyi Chen
Polyvinylpyrrolidone-iodine (PVP-I) is a broad-spectrum antimicrobial agent, but its effects on tendon-bone healing are unclear. The purpose of this study was to investigate the effects of PVP-I on bone marrow mesenchymal stem cells (BMSCs) in vitro and on tendon-bone healing in vivo. In this study, following investigation of the concentration-dependent effects of PVP-I on the viability and osteogenic differentiation of BMSCs, the appropriate concentration of PVP-I was selected for animal experiments. New Zealand white rabbits received autologous tendon transplantation with and without PVP-I treatment of the graft tendon...
June 2017: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/28585319/structurally-and-functionally-optimized-silk-fibroin-gelatin-scaffold-using-3d-printing-to-repair-cartilage-injury-in-vitro-and-in-vivo
#16
Weili Shi, Muyang Sun, Xiaoqing Hu, Bo Ren, Jin Cheng, Chenxi Li, Xiaoning Duan, Xin Fu, Jiying Zhang, Haifeng Chen, Yingfang Ao
Articular cartilage repair remains a great challenge for clinicians and researchers. Recently, there emerges a promising way to achieve one-step cartilage repair in situ by combining endogenic bone marrow stem cells (BMSCs) with suitable biomaterials using a tissue engineering technique. To meet the increasing demand for cartilage tissue engineering, a structurally and functionally optimized scaffold is designed, by integrating silk fibroin with gelatin in combination with BMSC-specific-affinity peptide using 3D printing (3DP) technology...
June 6, 2017: Advanced Materials
https://www.readbyqxmd.com/read/28584529/htert-immortalized-bone-mesenchymal-stromal-cells-expressing-rat-galanin-via-a-single-tetracycline-inducible-lentivirus-system
#17
Ke An, Hui-Ping Liu, Xiao-Long Zhong, David Y B Deng, Jing-Jun Zhang, Zhi-Heng Liu
The use of human telomerase reverse transcriptase-immortalized bone marrow mesenchymal stromal cells (hTERT-BMSCs) as vehicles to deliver antinociceptive galanin (GAL) molecules into pain-processing centers represents a novel cell therapy strategy for pain management. Here, an hTERT-BMSCs/Tet-on/GAL cell line was constructed using a single Tet-on-inducible lentivirus system, and subsequent experiments demonstrated that the secretion of rat GAL from hTERT-BMSCs/Tet-on/GAL was switched on and off under the control of an inducer in a dose-dependent manner...
2017: Stem Cells International
https://www.readbyqxmd.com/read/28583105/characteristics-and-potentials-of-stem-cells-derived-from-human-degenerated-nucleus-pulposus-potential-for-regeneration-of-the-intervertebral-disc
#18
Xiao-Chuan Li, Yong Tang, Jian-Hong Wu, Pu-Shan Yang, De-Li Wang, Di-Ke Ruan
BACKGROUND: Eliminating the symptoms during treatment of intervertebral disc degeneration (IVDD) is only a temporary solution that does not cure the underlying cause. A biological method to treat this disorder may be possible by the newly discovered nucleus pulposus derived stem cells (NPDCs). However, the uncertain characteristics and potential of NPDCs calls for a comprehensive study. METHODS: In the present study, nucleus pulposus samples were obtained from 5 patients with IVDD undergoing discectomy procedure and NPDCs were harvested using fluorescence activated cell sorting (FACS) by the co-expression of GD2(+) and Tie2(+)...
June 5, 2017: BMC Musculoskeletal Disorders
https://www.readbyqxmd.com/read/28580186/mash1-dependent-notch-signaling-pathway-regulates-gabaergic-neuron-like-differentiation-from-bone-marrow-derived-mesenchymal-stem-cells
#19
Qianfa Long, Qiang Luo, Kai Wang, Adrian Bates, Ashok K Shetty
GABAergic neuronal cell grafting has promise for treating a multitude of neurological disorders including epilepsy, age-related memory dysfunction, Alzheimer's disease and schizophrenia. However, identification of an unlimited source of GABAergic cells is critical for advancing such therapies. Our previous study implied that reprogramming of bone marrow-derived mesenchymal stem cells (BMSCs) through overexpression of the Achaete-scute homolog 1 (Ascl1, also called Mash1) could generate GABAergic neuron-like cells...
May 2017: Aging and Disease
https://www.readbyqxmd.com/read/28579894/osteogenic-differentiation-of-bone-marrow-mesenchymal-stem-cells-by-magnetic-nanoparticle-composite-scaffolds-under-a-pulsed-electromagnetic-field
#20
Jianghong Huang, Daming Wang, Jielin Chen, Wei Liu, Li Duan, Wei You, Weimin Zhu, Jianyi Xiong, Daping Wang
This study was conducted to investigate the effect of magnetic nanoparticle composite scaffold under a pulsed electromagnetic field on bone marrow mesenchymal stem cells (BMSCs), which was achieved by examining the biological behaviors of cell adhesion, proliferation and differentiation on the surface of the scaffolds. This may provide some experimental evidence for the use of magnetic nanoparticles in medical application. The magnetic nanoparticle composite scaffolds were evaluated and characterized by the following indexes: the cell proliferation was detected by the CCK-8 method, the alkaline phosphatase (ALP) activity was examined by a detection kit, and the expression of type I collagen and osteocalcin gene were evaluated by RT-PCR...
May 2017: Saudi Pharmaceutical Journal: SPJ: the Official Publication of the Saudi Pharmaceutical Society
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