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https://www.readbyqxmd.com/read/28432001/in-vivo-and-in-vitro-study-of-osteogenic-potency-of-endothelin-1-on-bone-marrow-derived-mesenchymal-stem-cells
#1
Long-Wei Hu, Xiao Wang, Xin-Qun Jiang, Li-Qun Xu, Hong-Ya Pan
Bone marrow-derived mesenchymal stem cells (BMSCs) are a major source of osteoblasts and are crucial for bone remolding and repair and thus they are widely used for tissue engineering applications. Tissue engineering in combination with gene therapy is considered as a promising approach in new bone regeneration. Endothelin-1(EDN-1)is produced by vascular endothelial cells which plays an important role during bone development. However, its role in BMSCs remains largely unknown. We established EDN-1 overexpressed BMSCs, proliferation ability and osteogenesis differentiation were detected respectively...
April 18, 2017: Experimental Cell Research
https://www.readbyqxmd.com/read/28427135/-effects-of-allogeneic-bone-marrow-mesenchymal-stem-cells-on-polarization-of-peritoneal-macrophages-in-rats-with-sepsis
#2
Y H Zheng, B Xiong, Y Y Deng, W Lai, S Y Zheng, H N Bian, Z A Liu, Z F Huang, C W Sun, H H Li, H M Luo, L H Ma, H X Chen
Objective: To explore the effects of allogeneic bone marrow mesenchymal stem cells (BMSCs) on polarization of peritoneal macrophages isolated from rats with sepsis induced by endotoxin/lipopolysaccharide (LPS). Methods: (1) BMSCs were isolated, cultured and purified from 5 SD rats with whole bone marrow adherent method. The third passage of cells were collected for morphologic observation, detection of expressions of stem cell surface markers CD29, CD44, CD45, and CD90 with flow cytometer, and identification of osteogenic and adipogenic differentiation...
April 20, 2017: Zhonghua Shao Shang za Zhi, Zhonghua Shaoshang Zazhi, Chinese Journal of Burns
https://www.readbyqxmd.com/read/28424584/hif-1%C3%AE-overexpression-improves-transplanted-bone-mesenchymal-stem-cells-survival-in-rat-mcao-stroke-model
#3
Bingke Lv, Feng Li, Jianbang Han, Jie Fang, Limin Xu, Chengmei Sun, Tian Hua, Zhongfei Zhang, Zhiming Feng, Xiaodan Jiang
Bone mesenchymal stem cells (BMSCs) death after transplantation is a serious obstacle impacting on the outcome of cell therapy for cerebral infarction. This study was aimed to investigate whether modification of BMSCs with hypoxia-inducible factor 1α (Hif-1α) could enhance the survival of the implanted BMSCs. BMSCs were isolated from Wistar rats, and were infected with Hif-1α-GFP lentiviral vector or Hif-1α siRNA. The modified BMSCs were exposed to oxygen-glucose deprivation (OGD) condition, cellular viability and apoptosis were then assessed...
2017: Frontiers in Molecular Neuroscience
https://www.readbyqxmd.com/read/28419760/bone-marrow-derived-stem-stromal-cells-and-adipose-tissue-derived-stem-stromal-cells-their-comparative-efficacies-and-synergistic-effects
#4
REVIEW
Gun-Il Im
Mesenchymal stem cells (MSCs) are heterogeneous cell populations that serve as reserves for tissue regeneration in the presence of disease or injury. Although MSCs are found in various tissues, bone marrow-derived stem/stromal cells (BMSCs) and adipose tissue-derived stem/stromal cells (ADSCs) have been most thoroughly investigated. Furthermore, ADSCs have recently emerged as an attractive source of MSCs due to their abundance and availability. BMSCs and ADSCs demonstrate similar morphological characteristics, but their in vitro characteristics and differentiation abilities appear to differ...
April 17, 2017: Journal of Biomedical Materials Research. Part A
https://www.readbyqxmd.com/read/28418864/bone-marrow-derived-mesenchymal-stem-cells-overexpressing-mir-21-efficiently-repair-myocardial-damage-in-rats
#5
Yan-Ling Zeng, Hao Zheng, Qiu-Ru Chen, Xiao-Hong Yuan, Jin-Hua Ren, Xiao-Feng Luo, Ping Chen, Zhe-Yao Lin, Shao-Zhen Chen, Xue-Qiong Wu, Min Xiao, Yong-Quan Chen, Zhi-Zhe Chen, Jian-Da Hu, Ting Yang
OBJECTIVE: We investigated the ability of bone marrow derived mesenchymal stem cells (BMSCs) overexpressing microRNA-21 (miR-21) to repair cardiac damage induced by anthracyclines in rats. METHODS: Sprague-Dawley (SD) rats of 2~3 weeks old were selected to isolate and culture BMSCs. A lentivirus harboring pLVX-miR-21 was generated and transfected into rat BMSCs. The rats were assigned into an untreated negative control group, and groups injected with adriamycin alone or with adriamycin followed by BMSCs, pLVX-BMSCs or pLVX-miR-21-BMSCs (n = 10 each)...
March 16, 2017: Oncotarget
https://www.readbyqxmd.com/read/28415701/wogonin-induces-retinal-neuron-like-differentiation-of-bone-marrow-stem-cells-by-inhibiting-notch-1-signaling
#6
Qinmeng Shu, Hong Zhuang, Jiawen Fan, Xin Wang, Gezhi Xu
Age-related macular degeneration and retinitis pigmentosa are major causes of irreversible vision loss in the elderly and, despite sustained efforts, current treatments are largely ineffective. Wogonin is a bioactive plant flavonoid possessing a range of beneficial properties, including neuroprotective effects. We investigated the ability of wogonin to promote retinal neuron-like differentiation of bone marrow stem cells (BMSCs) and assessed the involvement of Notch-1 signaling in this process. Cultured mouse BMSCs were left untreated or exposed to neurotrophic factors in the presence or absence of wogonin, and western blotting, RT-PCR and immunofluorescence were used to identify changes in molecular markers of stemness and neuroretinal differentiation...
March 10, 2017: Oncotarget
https://www.readbyqxmd.com/read/28415572/inhibition-of-iron-overload-induced-apoptosis-and-necrosis-of-bone-marrow-mesenchymal-stem-cells-by-melatonin
#7
Fan Yang, Yuan Li, Gege Yan, Tianyi Liu, Chao Feng, Rui Gong, Ye Yuan, Fengzhi Ding, Lai Zhang, Elina Idiiatullina, Valentin Pavlov, Zhenbo Han, Wenya Ma, Qi Huang, Ying Yu, Zhengyi Bao, Xiuxiu Wang, Bingjie Hua, Zhimin Du, Benzhi Cai, Lei Yang
Iron overload induces severe damage to several vital organs such as the liver, heart and bone, and thus contributes to the dysfunction of these organs. The aim of this study is to investigate whether iron overload causes the apoptosis and necrosis of bone marrow mesenchymal stem cells (BMSCs) and melatonin may prevent its toxicity. Perls' Prussion blue staining showed that exposure to increased concentrations of ferric ammonium citrate (FAC) induced a gradual increase of intracellular iron level in BMSCs. Trypan blue staining demonstrated that FAC decreased the viability of BMSCs in a concentration-dependent manner...
March 19, 2017: Oncotarget
https://www.readbyqxmd.com/read/28415442/small-intestinal-submucosa-a-potential-osteoconductive-and-osteoinductive-biomaterial-for-bone-tissue-engineering
#8
Mei Li, Chi Zhang, Mengjie Cheng, Qiaoqiao Gu, Jiyuan Zhao
SIS is an acellular, naturally occurring collagenous extracellular matrix (ECM) material with various bioactive factors, which broadly applied in tissue engineering in clinic. Several studies have applied SIS in bone tissue engineering to enhance bone regeneration in animal models. However, the mechanism was rarely investigated. The aim of the current study was to investigate the osteoconductivity and osteoinductivity of SIS scaffold to bone regeneration systematically and the potential mechanism. Our results showed that SIS scaffold with excellent biocompatibility was beneficial for cell attachment, proliferation, migration and osteogenic differentiation of various cells contributing to bone repair...
June 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28414206/wenshen-zhuanggu-formula-effectively-suppresses-breast-cancer-bone-metastases-in-a-mouse-xenograft-model
#9
Jia-Jia Li, Wei-Ling Chen, Jian-Yi Wang, Qian-Wen Hu, Zhen-Ping Sun, Shuai Zhang, Sheng Liu, Xiang-Hui Han
Wenshen Zhuanggu formula (WSZG) is a traditional Chinese medicine used as an adjuvant for the prevention of bone metastases in breast cancer patients. In this study we investigated the efficacy of WSZG in preventing bone metastases and the potential mechanisms in a mouse xenograft model of breast cancer bone metastases. This model was established by injection of human MDA-MB-231BO-Luc breast cancer cells alone or a mixture of the cancer cells with bone marrow-derived mesenchymal stem cells (BMSCs) into left ventricle of the heart in female nude mice...
April 17, 2017: Acta Pharmacologica Sinica
https://www.readbyqxmd.com/read/28411439/neuropeptide-y-enhances-proliferation-and-prevents-apoptosis-in-rat-bone-marrow-stromal-cells-in-association-with-activation-of-the-wnt-%C3%AE-catenin-pathway-in-vitro
#10
Jianqun Wu, Song Liu, Huan Meng, Tianyu Qu, Su Fu, Zhao Wang, Jianguo Yang, Dan Jin, Bin Yu
Neuropeptide Y (NPY) exhibits a critical but poorly understood regulatory signaling function and has been shown to promote proliferation, vascularization and migration in several types of cells and tissues. However, little is known about the specific role of NPY in the proliferation and apoptosis of bone marrow stromal cells (also known as bone marrow-derived mesenchymal stem cells, BMSCs), which contain a subpopulation of multipotent skeletal stem cells. Based on BrdU incorporation tests, Cell Counting Kit-8, flow cytometry, quantitative polymerase chain reaction and western blotting, we showed that NPY significantly promoted the proliferation of BMSCs in a concentration-dependent manner, with a maximal effect observed at a concentration of 10(-10)M for pro-proliferative and 10(-12)M for anti-apoptotic activities...
April 5, 2017: Stem Cell Research
https://www.readbyqxmd.com/read/28409921/mesenchymal-stem-cells-seeded-regenerated-silk-fibroin-complex-matrices-for-liver-regeneration-in-an-animal-model-of-acute-liver-failure
#11
Lijuan Xu, Shufang Wang, Xiang Sui, Yu Wang, Yinan Su, Li Huang, Yunwei Zhang, Zheng Chen, Qianqian Chen, Haitao Du, Yaopeng Zhang, Li Yan
The shortage of organ donation is the main limitation to liver transplantation as a treatment for the end-stage liver disease or acute liver failure. In order to develop an alternative therapy for acute liver failure, mesenchymal stem cells (MSCs) seeded regenerated silk fibroin (RSF) matrices were evaluated in vitro and in vivo. Adipose-derived mesenchymal stem cells (ADSCs) and bone marrow-derived mesenchymal stem cells (BMSCs) were planted and grown on RSF scaffolds to form scaffolds complex, respectively...
April 14, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28408732/glial-cell-line-derived-neurotrophic-factor-transfected-placenta-derived-versus-bone-marrow-derived-mesenchymal-cells-for-treating-spinal-cord-injury
#12
Yao Lu, Hui Gao, Man Zhang, Bing Chen, Huilin Yang
BACKGROUND Placenta-derived mesenchymal stem cells (PMSCs) were isolated from placenta and had differentiation and self-renewal potential. We transfected PMSCs with glial cell line-derived neurotrophic factor (GDNF) and compared their effect for repairing spinal cord injury (SCI) with that of GDNF-transfected bone marrow-derived mesenchymal stem cell (BMSC). MATERIAL AND METHODS The PMSCs were isolated from Sprague-Dawley rat placenta; BMSCs were isolated from Sprague-Dawley rat thigh bone marrow. Primary cultured BMSCs and PMSCs were uniformly spindle-shaped...
April 14, 2017: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
https://www.readbyqxmd.com/read/28404942/a-novel-bioactive-osteogenesis-scaffold-delivers-ascorbic-acid-%C3%AE-glycerophosphate-and-dexamethasone-in-vivo-to-promote-bone-regeneration
#13
Chao Wang, Xuecheng Cao, Yongxian Zhang
Ascorbic acid, β-glycerophosphate, and dexamethasone have been used in osteogenesis differentiation medium for in vitro cell culture, nothing is known for delivering these three bioactive compounds in vivo. In this study, we synthesized a novel bioactive scaffold by combining these three compounds with a lysine diisocyanate-based polyurethane. These bioactive compounds were released from the scaffold during the degradation process. The cell culture showed that the sponge-like structure in the scaffold was critical in providing a large surface area to support cell growth and all degradation products of the polymer were non-toxic...
February 28, 2017: Oncotarget
https://www.readbyqxmd.com/read/28400986/combining-injectable-plasma-scaffold-with-mesenchymal-stem-stromal-cells-for-repairing-infarct-cavity-after-ischemic-stroke
#14
Hongxia Zhang, Fen Sun, Jixian Wang, Luokun Xie, Chenqi Yang, Mengxiong Pan, Bei Shao, Guo-Yuan Yang, Shao-Hua Yang, Qichuan ZhuGe, Kunlin Jin
Stroke survivors are typically left with structural brain damage and associated functional impairment in the chronic phase of injury, for which few therapeutic options exist. We reported previously that transplantation of human embryonic stem cell (hESC)-derived neural stem cells together with Matrigel scaffolding into the brains of rats after focal ischemia reduced infarct volume and improved neurobehavioral performance. Matrigel is a gelatinous protein mixture extracted from mouse sarcoma cells, thus would not be approved for use as a scaffold clinically...
April 2017: Aging and Disease
https://www.readbyqxmd.com/read/28392353/decreased-nuclear-stiffness-via-fak-erk1-2-signaling-is-necessary-for-osteopontin-promoted-migration-of-bone-marrow-derived-mesenchymal-stem-cells
#15
Lingling Liu, Qing Luo, Jinghui Sun, Aoli Wang, Yisong Shi, Yang Ju, Yasuyuki Morita, Guanbin Song
Migration of bone marrow-derived mesenchymal stem cells (BMSCs) plays an important role in many physiological and pathological settings, including wound healing. During the migration of BMSCs through interstitial tissues, the movement of the nucleus must be coordinated with the cytoskeletal dynamics, which in turn affects the cell migration efficiency. Our previous study indicated that osteopontin (OPN) significantly promotes the migration of rat BMSCs. However, the nuclear behaviors and involved molecular mechanisms in OPN-mediated BMSC migration are largely unclear...
April 6, 2017: Experimental Cell Research
https://www.readbyqxmd.com/read/28386345/tgf-%C3%AE-mapk-signaling-mediates-the-effects-of-bone-marrow-mesenchymal-stem-cells-on-urinary-control-and-interstitial-cystitis-after-urinary-bladder-transplantation
#16
Ya Xiao, Ya-Jun Song, Bo Song, Chi-Bing Huang, Qing Ling, Xiao Yu
OBJECTIVE: This study aimed to explore the role of the transforming growth factor-β/mitogen activated protein kinase (TGF-β/MAPK) signaling pathway in the effects of bone marrow mesenchymal stem cells (BMSCs) on urinary control and interstitial cystitis in a rat model of urinary bladder transplantation. METHODS: A urinary bladder transplantation model was established using Sprague-Dawley rats. Rats were assigned to normal (blank control), negative control (phosphate-buffered saline injection), BMSCs (BMSC injection), sp600125 (MAPK inhibitor injection), or protamine sulfate (protamine sulfate injection) groups...
2017: American Journal of Translational Research
https://www.readbyqxmd.com/read/28386334/are-sertoli-cells-a-kind-of-mesenchymal-stem-cells
#17
Daoyuan Gong, Chunfu Zhang, Tao Li, Jiahui Zhang, Nannan Zhang, Zehua Tao, Wei Zhu, Xiaochun Sun
OBJECTIVE: Sertoli cells (SCs) are a major component of testis which secrete a variety of cytokines and immunosuppressive factors, providing nutritional support and immune protection for sperm growth and development. The purpose of this study was to investigate the relationship between SCs and bone marrow mesenchymal stem cells (BMSCs) in order to provide a theoretical basis for better application of SCs. METHODS: We used the adherence method to isolate Sprague-Dawley rat SCs and BMSCs...
2017: American Journal of Translational Research
https://www.readbyqxmd.com/read/28386176/derivation-and-characterization-of-sheep-bone-marrow-derived-mesenchymal-stem-cells-induced-with-telomerase-reverse-transcriptase
#18
Xuemin Zhu, Zongzheng Liu, Wen Deng, Ziqiang Zhang, Yumei Liu, Lan Wei, Yuling Zhang, Liutao Zhou, Yuzhu Wang
Bone marrow mesenchymal stem cells (BMSCs) are a type of adult stem cells with a wide range of potential applications. However, BMSCs have a limited life cycle under normal culturing conditions, which has hindered further study and application. Many studies have confirmed that cells modified by telomerase reverse transcriptase (TERT) can maintain the ability to proliferate in vitro over a long period of time. In this study, we constructed a gene expression vector to transfer TERT into sheep BMSCs, and evaluated whether the TERT cell strain was successfully transferred...
March 2017: Saudi Journal of Biological Sciences
https://www.readbyqxmd.com/read/28373667/bone-marrow-derived-mesenchymal-stem-cells-bmscs-repair-acute-necrotized-pancreatitis-by-secreting-microrna-9-to-target-the-nf-%C3%AE%C2%BAb1-p50-gene-in-rats
#19
Daohai Qian, Ge Wei, Chenglei Xu, Zhigang He, Jie Hua, Jian Li, Qili Hu, Shengping Lin, Jian Gong, Hongbo Meng, Bo Zhou, Hongfei Teng, Zhenshun Song
Acute pancreatitis (AP) is a common acute abdominal disease, 10-20% of which can evolve into severe AP (SAP) causing significant morbidity and mortality. Bone marrow-derived mesenchymal stem cells (BMSCs) have the potential of repairing SAP, but the detailed mechanism remains unknown. We demonstrate here that microRNA-9 (miR-9) modified BMSCs (pri-miR-9-BMSCs) can significantly reduce the pancreatic edema, infiltration, hemorrhage, necrosis, the release of amylase and lipase. Meanwhile, decreased local/systemic inflammatory response (TNF-α↓, IL-1β↓, IL-6↓, HMGB1↓, MPO↓, CD68↓, IL-4↑, IL-10↑, and TGF-β↑) and enhanced regeneration of damaged pancreas (Reg4↑, PTF1↑, and PDX1↑) are also promoted...
April 3, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28370348/microvesicles-shuttled-mir-200b-attenuate-experimental-colitis-associated-intestinal-fibrosis-by-inhibiting-the-development-of-emt
#20
Jia Yang, Cheng-Zhi Zhou, Rui Zhu, Heng Fan, Xing-Xing Liu, Xue-Yun Duan, Qing Tang, Zhe-Xing Shou, Dong-Mei Zuo
BACKGROUND: Epithelial-mesenchymal transition (EMT), characterized by the decrease of E-Cad and increase of vimentin and α-SMA, was demonstrated to participate in inflammatory bowel disease (IBD) related fibrosis. MiR-200b plays an anti-fibrosis role in inhibiting EMT by targeting ZEB1 and ZEB2. But the stability of exogenous miR-200b in blood limits its application. Microvesicles (MVs), which can transfer miRNAs among cells and prevent them from degradation, may provide an excellent transport system for the delivery of miR-200b in the treatment of fibrosis...
March 31, 2017: Journal of Gastroenterology and Hepatology
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