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https://www.readbyqxmd.com/read/29788550/usp22-acts-as-an-oncoprotein-to-maintain-glioma-malignancy-through-deubiquitinating-bmi1-for-stabilization
#1
Guan-Zhong Qiu, Xiao-Yuan Mao, Yue Ma, Xing-Chun Gao, Zhen Wang, Ming-Zhu Jin, Wei Sun, Yong-Xiang Zou, Jing Lin, Hua-Lin Fu, Wei-Lin Jin
USP22 is a member of "death-from-cancer" signature, which plays a key role in cancer progression. Although previous evidence has shown that USP22 is overexpressed and correlated with poor prognosis in glioma. The effect and mechanism of USP22 in glioma malignancy especially cancer stemness remain elusive. Here, we find USP22 is more enriched in stem-like tumorspheres than differentiated glioma cells. USP22 knockdown inhibits cancer stemness in glioma cell lines. With a cell-penetrating TAT-tag protein, BMI1, a robust glioma stem-cell marker, is found to mediate the effect of USP22 on glioma stemness...
May 22, 2018: Cancer Science
https://www.readbyqxmd.com/read/29775627/acquisition-of-tumorigenic-potential-and-therapeutic-resistance-in-cd133-subpopulation-of-prostate-cancer-cells-exhibiting-stem-cell-like-characteristics
#2
Rajnee Kanwal, Sanjeev Shukla, Ethan Walker, Sanjay Gupta
The role of CD133 (Prominin-1) as a cancer stem cell marker may be useful for therapeutic approaches and prognostication in prostate cancer patients. We investigated the stem-cell-related function and biological features of a subpopulation of CD133+ cells isolated from established primary human prostate cancer cell lines. The CD133+ cells sorted from human prostate cancer 22Rv1 exhibited high clonogenic and tumorigenic capabilities, sphere forming capacity and serially reinitiated transplantable tumors in NOD-SCID mice...
May 15, 2018: Cancer Letters
https://www.readbyqxmd.com/read/29772566/the-epigenetic-factor-kdm2b-regulates-emt-and-small-gtpases-in-colon-tumor-cells
#3
Nefeli Zacharopoulou, Anna Tsapara, Galatea Kallergi, Evi Schmid, Saad Alkahtani, Saud Alarifi, Philip N Tsichlis, Sotirios C Kampranis, Christos Stournaras
BACKGROUND/AIMS: The epigenetic factor KDM2B is a histone demethylase expressed in various tumors. Recently, we have shown that KDM2B regulates actin cytoskeleton organization, small Rho GTPases signaling, cell-cell adhesion and migration of prostate tumor cells. In the present study, we addressed its role in regulating EMT and small GTPases expression in colon tumor cells. METHODS: We used RT-PCR for the transcriptional analysis of various genes, Western blotting for the assessment of protein expression and immunofluorescence microscopy for visualization of fluorescently labeled proteins...
May 14, 2018: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/29753810/dendrosomal-nanocurcumin-and-exogenous-p53-can-act-synergistically-to-elicit-anticancer-effects-on-breast-cancer-cells
#4
Narges Baghi, Babak Bakhshinejad, Reihaneh Keshavarz, Sadegh Babashah, Majid Sadeghizadeh
Triple-negative breast cancer (TNBC) constitutes an important histological subtype of breast cancer with a highly metastatic phenotype. The aim of the current study was to investigate the possible synergism between dendrosomal nanocurcumin (DNC) and exogenously delivered p53 in producing anticancer effects on a TNBC cell line. MTT assay was exploited to determine the viability of MDA-MB-231 cells against DNC and measure the impact of p53 overexpresssion on DNC-related cytotoxicity. Annexin-V/PI staining followed by flow cytometry and wound healing assay were used to evaluate the effects of DNC and exogenous p53, alone and in combination, on apoptosis induction and migratory capacity of MDA-MB-231 cells, respectively...
May 10, 2018: Gene
https://www.readbyqxmd.com/read/29749669/in-silico-analysis-of-single-cell-rna-sequencing-data-from-3-and-7-days-old-mouse-spermatogonial-stem-cells-to-identify-their-differentially-expressed-genes-and-transcriptional-regulators
#5
Sajjad Sisakhtnezhad
Spermatogonial stem cells (SSCs), which are at the basis of spermatogenesis process, are valuable cells with different applications in biotechnology and regenerative medicine. Understanding the molecular basis of SSC self-renewal and differentiation at various developmental stages of the male organism is crucial to find key factors in the SSCs fate and function. Therefore, this study was aimed to use single-cell RNA-sequencing dataset analysis for identification of differentially expressed genes (DEGs) and their regulators in 3 and 7 days old mouse-derived single SSCs (mSSCs)...
May 11, 2018: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/29749381/effect-of-bmi1-over-expression-on-gene-expression-in-adult-and-embryonic-murine-neural-stem-cells
#6
Mythily Ganapathi, Nathan C Boles, Carol Charniga, Steven Lotz, Melissa Campbell, Sally Temple, Randall H Morse
The ability of isolated neural stem cells (NSCs) to proliferate as neurospheres is indicative of their competence as stem cells, and depends critically on the polycomb group (PcG) member Bmi1: knockdown of Bmi1 results in defective proliferation and self-renewal of isolated NSCs, whereas overexpression of Bmi1 enhances these properties. Here we report genome-wide changes in gene expression in embryonic and adult NSCs (eNSCs and aNSCs) caused by overexpression of Bmi1. We find that genes whose expression is altered by perturbations in Bmi1 levels in NSCs are mostly distinct from those affected in other multipotent stem/progenitor cells, such as those from liver and lung, aside from a small core of common targets that is enriched for genes associated with cell migration and mobility...
May 10, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29744869/isolation-and-identification-of-chemotherapy-enriched-sphere-forming-cells-from-a-patient-with-gastric-cancer
#7
Vahid Bagheri, Bahram Memar, Ramezan Behzadi, Mohsen Aliakbarian, Ali Jangjoo, Mostafa Mehrabi Bahar, Samaneh Talebi, Mehran Gholamin, Mohammad R Abbaszadegan
Gastric cancer (GC) is the third and fifth cause of cancer-associated mortality for men and women throughout the world, respectively. Despite the use of surgery and chemotherapy for GC therapy, there are no efficient therapeutic protocols for it to date. Cancer stem cells (CSCs) due to their pivotal role in tumor initiation, growth, progression, invasion, distant metastasis, recurrence and resistance to anticancer drugs are very appealing targets for cancer therapies. Here, we isolated and identified CSCs from a chemotherapy-treated patient...
May 10, 2018: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/29726283/lactobacillus-plantarum-is-10506-activates-intestinal-stem-cells-in-a-rodent-model
#8
A F Athiyyah, A Darma, R Ranuh, W Riawan, A Endaryanto, F A Rantam, I S Surono, S M Sudarmo
This study investigated the probiotic effect of Lactobacillus plantarum IS-10506 in activating and regenerating leucine-rich repeat-containing G-protein-coupled receptor (Lgr)5- and B lymphoma Moloney murine leukaemia virus insertion region (Bmi)1-expressing intestinal stem cells in rodents following Escherichia coli serotype 055:B5 lipopolysaccharide (LPS) exposure. Male Sprague-Dawley rats (n=64) were randomised into control (KN), LPS (KL), probiotic + LPS (KL-Pr), and sequential probiotic + LPS + probiotic (KPR-7L) groups...
May 4, 2018: Beneficial Microbes
https://www.readbyqxmd.com/read/29721964/the-evaluation-of-bmi1-posttranslational-modifications-during-retinal-degeneration-to-understand-bmi1-action-on-photoreceptor-death-execution
#9
Martial K Mbefo, Yvan Arsenijevic
Retinitis Pigmentosa (RP) is a class of hereditary retinal dystrophy associated with gradual visual failure and a subsequent loss of light-sensitive cells in the retina, leading to blindness. Many mutated genes were found to be causative of this disease. Despite a number of compiling efforts, the process of cell death in photoreceptors remains to be clearly elucidated. We recently reported an abnormal cell cycle reentry in photoreceptors undergoing degeneration in Rd1 mice, a model of RP, and identified the polycomb repressive complex 1 (PRC1) core component BMI1 as a critical molecular factor orchestrating the cell death mechanism...
2018: Advances in Experimental Medicine and Biology
https://www.readbyqxmd.com/read/29700121/genetic-lineage-tracing-of-non-myocyte-population-by-dual-recombinases
#10
Yan Li, Lingjuan He, Xiuzhen Huang, Shirin Issa Bhaloo, Huan Zhao, Shaohua Zhang, Wenjuan Pu, Xueying Tian, Yi Li, Qiaozhen Liu, Wei Yu, Libo Zhang, Xiuxiu Liu, Kuo Liu, Juan Tang, Hui Zhang, Dongqing Cai, Ralf H Adams, Qingbo Xu, Kathy O Lui, Bin Zhou
Background -Whether the adult mammalian heart harbors cardiac stem cells (CSCs) for regeneration of cardiomyocytes is an important yet contentious topic in the field of cardiovascular regeneration. The putative myocyte stem cell populations recognized without specific cell markers such as the cardiosphere-derived cells or with markers such as Sca1+ , Bmi1+ , Isl1+ or Abcg2+ CSCs have been reported. Moreover, it remains unclear whether putative CSCs with unknown or unidentified markers exist and give rise to de novo cardiomyocytes in the adult heart...
April 26, 2018: Circulation
https://www.readbyqxmd.com/read/29685168/role-of-bmi1-in-epithelial-ovarian-cancer-investigated-via-the-crispr-cas9-system-and-rna-sequencing
#11
Qianying Zhao, Qiuhong Qian, Dongyan Cao, Jiaxin Yang, Ting Gui, Keng Shen
BACKGROUND: B-cell-specific Moloney murine leukemia virus integration site 1 (BMI1) might be an appropriate biomarker in the management of epithelial ovarian cancer (EOC). However, the biological role of BMI1 and its relevant molecular mechanism needs further elaboration. Clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 system is an excellent genome-editing tool and is scarcely used in EOC studies. METHODS: We first applied CRISPR/Cas9 technique to silence BMI1 in EOC cells; thereafter we accomplished various in vivo and in vitro experiments to detect biological behaviors of ovarian cancer cells, including MTT, flow cytometry, Transwell, real-time polymerase chain reaction and western blotting assays, etc...
April 23, 2018: Journal of Ovarian Research
https://www.readbyqxmd.com/read/29669323/long-intergenic-noncoding-rna-00152-promotes-glioma-cell-proliferation-and-invasion-by-interacting-with-mir-16
#12
Xin Chen, Deheng Li, Yang Gao, Wei Tang, Lao Iw, Yiqun Cao, Bin Hao
BACKGROUND/AIMS: Long noncoding RNAs (lncRNAs) are a novel class of protein-noncoding transcripts that are aberrantly expressed in multiple diseases including cancers. LINC00152 has been identified as an oncogene involved in many kinds of cancer; however, its expression pattern and function in human glioma remain unclear. METHODS: Quantitative real-time polymerase chain reaction was carried out to measure LINC00152 expression in human glioma cell lines and tissues...
April 13, 2018: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/29666061/deptor-is-a-novel-target-of-wnt-%C3%AE-catenin-c-myc-and-contributes-to-colorectal-cancer-cell-growth
#13
Qingding Wang, Yuning Zhou, Piotr Rychahou, Jennifer W Harris, Yekaterina Y Zaytseva, Jinpeng Liu, Chi Wang, Heidi L Weiss, Chunming Liu, Eun Y Lee, B Mark Evers
Activation of the Wnt/β-catenin signaling pathway drives colorectal cancer (CRC) growth by deregulating expression of downstream target genes including the c-Myc proto-oncogene. The critical targets that mediate the functions of oncogenic c-Myc in CRC have yet to be fully elucidated. Previously, we showed that activation of PI3K/Akt/mTOR contributes to CRC growth and metastasis. Here we show that Deptor, a suppressor of mTOR, is a direct target of Wnt/β-catenin/c-Myc signaling in CRC cells. Inhibition of Wnt/β-catenin or knockdown of c-Myc decreased, while activation of Wnt/β-catenin or overexpression of c-Myc increased, the expression of Deptor...
April 17, 2018: Cancer Research
https://www.readbyqxmd.com/read/29626519/polycomb-complex-protein-bmi1-confers-resistance-to-tamoxifen-in-estrogen-receptor-positive-breast-cancer
#14
Diane Ojo, Xiaozeng Lin, Ying Wu, Jessica Cockburn, Anita Bane, Damu Tang
We report that BMI1 promotes tamoxifen resistance in estrogen receptor (ER)-positive breast cancer (BC). BMI1 overexpression conferred MCF7 and TD47 cells resistance to tamoxifen; BMI1 knockdown sensitized the process. In MCF7-derived tamoxifen resistant cells, BMI1 expression was upregulated and BMI1 knockdown reduced the resistance. BMI1 is an oncogene; its oncogenic activity is attributed to BMI1-stimulated E3 ubiquitin ligase activity, a process that requires BMI1's ring finger (RF) domain. However, a BMI1 mutant without RF conferred tamoxifen resistance...
April 4, 2018: Cancer Letters
https://www.readbyqxmd.com/read/29607565/prognostic-implications-of-cd44-nanog-oct4-and-bmi1-expression-in-tongue-squamous-cell-carcinoma
#15
Maria Fernanda Setúbal Destro Rodrigues, Flávia Caló de Aquino Xavier, Nathália Paiva Andrade, Camila Lopes, Lucyene Miguita Luiz, Bruno Tavares Sedassari, Ana Melissa Ccopa Ibarra, Rossana Verónica Mendoza López, Claudia Kliemann Schmerling, Raquel Ajub Moyses, Eloiza Elena Tajara da Silva, Fabio Daumas Nunes
BACKGROUND: Tongue squamous cell carcinoma (SCC) contains a cell subpopulation referred to as cancer stem cells (CSCs), which are responsible for tumor growth, metastasis, and resistance to chemotherapy and radiotherapy. The CSC markers have been used to isolate these cells and as biomarkers to predict overall survival. METHODS: The CSC markers CD44, NANOG, OCT4, and BMI1 were investigated using reverse transcriptase-quantitative polymerase chain reaction (RT-qPCR) and immunohistochemistry and correlated with clinicopathological parameters...
April 1, 2018: Head & Neck
https://www.readbyqxmd.com/read/29601474/lgr5-and-bmi1-increase-pig-intestinal-epithelial-cell-proliferation-by-stimulating-wnt-%C3%AE-catenin-signaling
#16
Xiang-Guang Li, Zhe Wang, Rong-Qiang Chen, Hou-Long Fu, Chun-Qi Gao, Hui-Chao Yan, Guang-Xu Xing, Xiu-Qi Wang
Leucine-rich repeat-containing G protein-coupled receptor 5 (LGR5) and B-cell-specific Moloney murine leukemia virus insertion site 1 (BMI1) are markers of fast-cycling and quiescent intestinal stem cells, respectively. To determine the functions of these proteins in large animals, we investigated their effects on the proliferation of intestinal epithelial cells from pigs. Our results indicated that LGR5 and BMI1 are highly conserved proteins and that the pig proteins have greater homology with the human proteins than do mouse proteins...
March 30, 2018: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29574252/targeting-long-noncoding-rna-hmmr-as1-suppresses-and-radiosensitizes-glioblastoma
#17
Junyang Li, Xiangjun Ji, Handong Wang
Emergent evidences revealed that long noncoding RNAs (lncRNAs) participate in neoplastic progression. HMMR is an oncogene that is highly expressed in glioblastoma (GBM) and supports GBM growth. Whether lncRNAs regulate HMMR in GBM remains unknown. Herein, we identify that an HMMR antisense lncRNA, HMMR-AS1, is hyperexpressed in GBM cell lines and stabilizes HMMR mRNA. Knockdown of HMMR-AS1 reduces HMMR expression; inhibits cell migration, invasion, and mesenchymal phenotypes; and suppresses GBM cell growth both in vitro and in vivo...
March 22, 2018: Neoplasia: An International Journal for Oncology Research
https://www.readbyqxmd.com/read/29559852/-bmi1-deficient-mice-exhibit-male-infertility
#18
Xiuliang Dai, Qian Zhang, Zhenzhen Yu, Weiwei Sun, Rong Wang, Dengshun Miao
Previous studies have demonstrated that the polycomb repressor Bmi1 is universally expressed in all types of testicular cells and might regulate the spermatogonia proliferation, however, it is unclear whether Bmi1 plays a critical role in maintaining normal male fertility in vivo. To answer this question, we first confirmed that Bmi1 is universally expressed in all types of testicular cells and found that the gene relative expression levels of Bmi1 in testis were the highest relative to other organs. Then we investigated the role of Bmi1 in maintaining normal male fertility using Bmi1 knockout male mouse model...
2018: International Journal of Biological Sciences
https://www.readbyqxmd.com/read/29559833/downregulation-of-mir-203a-disinhibits-bmi1-and-promotes-growth-and-proliferation-of-keratinocytes-in-cholesteatoma
#19
Jian Zang, Lian Hui, Ning Yang, Bo Yang, Xuejun Jiang
Background: Keratinocytes are the predominant cell type in a cholesteatoma, and microRNA (miR)-203a has been shown to be essential for the growth and differentiation of keratinocytes. The regulatory mechanisms of miR-203a and Bmi1-the predicted target of miR-203a that is associated with cholesteatoma-have not been clarified. Methods: Real-time PCR and western blot were carried out for the detection of miRNAs, mRNAs, and proteins, including miR-203a, Bmi1, and phosphorylated (p-)Akt. Immunohistochemical staining was applied to observe the expression and distribution of Bmi1 and of p-Akt in cholesteatoma and in control retroauricular skin...
2018: International Journal of Medical Sciences
https://www.readbyqxmd.com/read/29528145/timosaponin-a-iii-inhibits-oncogenic-phenotype-via-regulation-of-pcg-protein-bmi1-in-breast-cancer-cells
#20
Joseph E Gergely, Armond E Dorsey, Goberdhan P Dimri, Manjari Dimri
Polycomb group (PcG) protein BMI1 is an important regulator of oncogenic phenotype and is often overexpressed in several human malignancies including breast cancer. Aberrant expression of BMI1 is associated with metastasis and poor prognosis in cancer patients. At present, therapy reagents that can efficiently inhibit the expression of BMI1 are not very well known. Here, we report that Timosaponin A-III (TA-III), a steroidal saponin obtained from the rhizomes of an herb, Anemarrhena asphodeloides, strongly inhibits expression of BMI1 in breast cancer cells...
March 12, 2018: Molecular Carcinogenesis
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