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Lingkun Zuo, Huanying Zhao, Ronghui Yang, Liyong Wang, Hui Ma, Xiaoxue Xu, Ping Zhou, Lu Kong
We have previously reported a heterogeneous expression pattern of the nuclear membrane protein lamin A/C in low- and high-Gleason score (GS) prostate cancer (PC) tissues, and we have now found that this change is not associated with LMNA mutations. This expression pattern appears to be similar to the process of epithelial to mesenchymal transition (EMT) or to that of mesenchymal to epithelial transition (MET). The role of lamin A/C in EMT or MET in PC remains unclear. Therefore, we first investigated the expression levels of and the associations between lamin A/C and several common EMT markers, such as E-cadherin, N-cadherin, β-catenin, snail, slug and vimentin in PC tissues with different GS values and in different cell lines with varying invasion abilities...
April 14, 2018: Gene
Kang He, Guoqing Duan, Yanyang Li
Neuroblastoma (NB) is the most predominant extracranial solid tumor of infancy in the world. However, current chemotherapy has limited efficacy for more advanced stages of NB due to acquired chemoresistance or acute toxicity in NB patients. Therefore, effective novel anti-NB drugs are desperately needed. The present study aimed to investigate the effects of dehydroeffusol (DHE), a phenanthrene isolated from J. effuses, on NB cells and its underlying mechanism. The results showed that DHE treatment effectively inhibited NB cell viability in a dose-dependent manner...
April 14, 2018: European Journal of Pharmacology
Dhamotharan Pattarayan, Ayyanar Sivanantham, Ramalingam Bethunaickan, Rajaguru Palanichamy, Subbiah Rajasekaran
In response to tissue injury, fibroblasts migrate into the wound, where they undergo proliferation and differentiation. The persistence of these differentiated fibroblasts (myofibroblasts) is associated with excessive scarring in various organs. We aimed to investigate the effects of Tannic acid (TA) on fibroblast proliferation and differentiation, and found that TA inhibited fibroblast differentiation as assessed by reduced expression of α-smooth muscle actin, N-cadherin, and type-1-collagen. TA also prevented the TGF-β1-induced alteration in the expression of two classes of genes involved in the remodeling of extracellular matrix (ECM) proteins, namely matrix metalloproteinases (Mmp-2 and -9) and tissue inhibitors of metalloproteinases (Timp-1 and -3)...
April 17, 2018: Journal of Cellular Biochemistry
Yi Zhang, Yi Cheng, Cong Chen, Qi Liu, Xinyu Bi, Lei Duan, Jiamin Liu, Mengqi Wan, Lanye Huang, Ke Hu
Tumor metastasis and invasiveness are research hotspots in tumor therapy. Previous research demonstrated that cells expressing high level of CD36 might initiate metastasis in certain human oral carcinomas cell lines. Multicellular spheroids (MCS) culture technique has been extensively studied both in fundamental and applied research. In our previous research, we invented novel 3D anisotropic magnetic hydrogels (AMHs) which were composed of magnetic nanoparticle assemblies and biocompatible hydrogels. We further applied them as cell culture substrate in vitro...
March 1, 2018: Journal of Biomedical Nanotechnology
Fateme Karimi Dermani, Razieh Amini, Massoud Saidijam, Rezvan Najafi
Colorectal cancer (CRC) is the most frequently diagnosed cancer and the most common gastrointestinal cancer worldwide. Due to the presence of populations of cancer stem cells (CSCs) that cause recurrence, the possibility of cancer treatment is very low. The aim of current study was to evaluate the inhibitory role of miR-200c on EMT, CSCs markers and β-catenin in HCT-116 and SW48 cell lines. The expression of miR-200c, EMT-related genes, CSCs markers, and β-catenin were quantified by qRT-PCR. Further, expression of β-catenin and EMT-related proteins, and migration were analyzed by Western blot, and migration assay kit, respectively...
April 16, 2018: Journal of Cellular Biochemistry
Kazuma Fujikake, Masato Sawada, Takao Hikita, Yayoi Seto, Naoko Kaneko, Vicente Herranz-Pérez, Natsuki Dohi, Natsumi Homma, Satoshi Osaga, Yuchio Yanagawa, Toshihiro Akaike, Jose Manuel García-Verdugo, Mitsuharu Hattori, Kazuya Sobue, Kazunobu Sawamoto
In the rodent olfactory system, neuroblasts produced in the ventricular-subventricular zone of the postnatal brain migrate tangentially in chain-like cell aggregates toward the olfactory bulb (OB) through the rostral migratory stream (RMS). After reaching the OB, the chains are dissociated and the neuroblasts migrate individually and radially toward their final destination. The cellular and molecular mechanisms controlling cell-cell adhesion during this detachment remain unclear. Here we report that Fyn, a non-receptor tyrosine kinase, regulates the detachment of neuroblasts from chains in the male and female mouse OB...
April 16, 2018: Journal of Neuroscience: the Official Journal of the Society for Neuroscience
Tianxiao Xu, Meng Ma, Zhihong Chi, Lu Si, Xinan Sheng, Chuanliang Cui, Jie Dai, Sifan Yu, Junya Yan, Huan Yu, Xiaowen Wu, Huan Tang, Jiayi Yu, Yan Kong, Jun Guo
G-2 and S-phase expressed 1 (GTSE1) regulates cell cycle progression in human cancers. However, its significance and mechanism of action in acral melanoma (AM) remain unknown. In this study, we found that GTSE1 expression was upregulated in advanced stage/metastatic AM tissues and metastatic cell lines, and correlated with higher stage (P = 0.028) and poor disease-free survival (DFS) in patients with AM (P = 0.003). Cox regression assays validated GTSE1 expression to be an independent prognostic factor of DFS for patients with AM (P = 0...
April 16, 2018: Cancer Science
Kun Qian, Laiyu Sun, Guoqing Zhou, Haixia Ge, Yue Meng, Jingfen Li, Xiao Li, Xinqiang Fang
Sodium phenylbutyrate (SPB) as a salt of 4-phenylbutyric acid (4-PBA) has been reported to be an ammonia scavenger, histone deacetylase inhibitor, and an endoplasmic reticulum stress inhibitor in various diseases, including neurological diseases, inflammatory disorders, and carcinogenesis. Although phenylbutyrate showed effective antitumor properties in many cancers, its role in oral squamous cell carcinoma (OSCC) remains further characterized. Thus, the OSCC cell lines CAL27, HSC3, and SCC4 were treated with a series of doses of SPB for different times...
April 16, 2018: Cancer Biotherapy & Radiopharmaceuticals
Zhongbo Chen, Jianqing Zhu, Yiming Zhu, Junjian Wang
MicroRNAs (miRNAs), a group of short (~20 nt) non‑coding RNAs, play critical roles in the development and progression of ovarian cancer (OC). The role of miR‑616, a recently identified cancer-associated miRNA, has never been examined in OC before. The present study demonstrated that the level of miR‑616 was increased in OC tissues. A high miR‑616 level was associated with poor tumor differentiation and advanced tumor-node-metastasis (TNM) stage. Survival analysis revealed that an elevated level of miR‑616 was associated with poor prognosis of OC patients as demonstrated by decreased overall survival (OS) and disease‑free survival (DFS)...
April 12, 2018: Oncology Reports
Qianfeng Zhuang, Weiping Luo, Mingran Zhang, Min Fan, Hao Lu, Renfang Xu, Xiaozhou He
Calpain small subunit 1 (Capn4) has been shown to correlate with the metastasis/invasion of clear cell renal cell carcinoma (ccRCC). This study aimed to further elucidate the molecular mechanisms underlying Capn4-mediated ccRCC progression. The messenger RNA expression levels in ccRCC cells were measured by quantitative real-time PCR. The effects of Capn4 on cell adhesion, invasion, and migration were examined by cell adhesion assay, cell invasion assay, and wound-healing assay, respectively. The protein levels were detected by western blot analysis...
April 10, 2018: Acta Biochimica et Biophysica Sinica
Sadia Afrin, Francesca Giampieri, Massimiliano Gasparrini, Tamara Y Forbes-Hernández, Danila Cianciosi, Patricia Reboredo-Rodriguez, Piera Pia Manna, Jiaojiao Zhang, Josè L Quiles, Maurizio Battino
Despite its high content of phenolic compounds, the chemopreventive activity of Manuka honey (MH) is still elusive. The aim of the present work was to evaluate the effects of MH on oxidative stress, antioxidant enzymes, cellular metabolism and the metastatic ability in HCT-116 and LoVo cells, paying particular attention to the molecular mechanisms involved. We observed a strong induction of oxidative stress after MH treatment since it augmented the accumulation of reactive oxygen species and increased the damage to proteins, lipids and DNA...
April 12, 2018: Food & Function
Z-G Gu, G-H Shen, J-H Lang, W-X Huang, Z-H Qian, J Qiu
OBJECTIVE: To investigate the effect of long non-coding RNA URHC on the proliferation, apoptosis and invasion of colorectal cancer cells. PATIENTS AND METHODS: The expression of lncRNA-URHC in tissues and cells was tested by Real-time quantitative PCR. The expression of lncRNA-URHC was down-regulated by RNA interference (siRNA). The Real-time quantitative polymerase chain reaction (PCR) method was used to detect the interference efficiency. Cell counting kit-8 (CCK-8), flow cytometry, and transwell were used to detect the effect of lncRNA-URHC on the proliferation, apoptosis and invasion of colorectal cancer cells...
March 2018: European Review for Medical and Pharmacological Sciences
Naoyuki Chosa, Akira Ishisaki
Mesenchymal stem cells (MSCs) retain the ability to self-renew and differentiate into mesenchymal cells. Therefore, human MSCs are suitable candidates for use in regenerative medicine and cell therapies. Upon activation by tissue damage, MSCs contribute to tissue repair through a multitude of processes such as self-renewal, migration, and differentiation. However, loss of self-renewal and multi-lineage differentiation potential occurs at a high rate during cell doubling. Effective MSC therapies require the establishment of new techniques that preserve MSC multipotency after lengthy cell expansions...
February 2018: Japanese Dental Science Review
Hongdan Li, Haoqi Wang, Zhen Ren
BACKGROUND/AIMS: This study aims to explore the effects of microRNA-214-5p (miR-214-5p) on the invasion and migration of Hepatocellular Carcinoma cells (HCC). METHODS: Hepatocellular Carcinoma tissues and adjacent normal tissues from 44 hepatocellular carcinoma patients were prepared for this study. The HepG2 and BEL-7402 cells were transfected with miR-214-5p mimic and inhibitor. qRT-PCR was performed to detect the expressions of miR-214-5p. Transwell assays were used to detect the invasion and migration assays in HepG2 and BEL-7402 cells...
March 29, 2018: Cellular Physiology and Biochemistry
Shasha Wu, Feixiang Wu, Zheng Jiang
Colorectal cancer (CRC) is one of the most common types of tumor worldwide. The morbidity and mortality rates of CRC have increased significantly in adults <50 years of age. In the present study, the effects of homeobox A6 (HOXA6) on the proliferation, apoptosis, migration and invasion of CRC cells were investigated. The results of reverse transcription-quantitative polymerase chain reaction (RT-qPCR) analysis demonstrated that the expression of HOXA6 in CRC tumor tissue was higher than that in adjacent normal tissue...
April 2, 2018: International Journal of Oncology
Xiaowei Fu, Yaqiong Zhu, Bingbing Zheng, Yeqing Zou, Chao Wang, Peng Wu, Jun Wang, Haimin Chen, Pengcheng Du, Bo Liang, Lu Fang
Kinesin family member C1 (KIFC1, also known as HSET) is a minus end-directed motor protein, which is critical in centrosome clustering. The present study investigated the expression of KIFC1 in paired hepatocellular carcinoma (HCC) tissues and adjacent non-cancerous tissues from 91 patients by immunohistochemical analysis; clinical data were concomitantly collected. KIFC1 was expressed at high levels in HCC tissues, compared with that in peritumoral tissues (54.9 vs. 14.3%; P<0.01), and its expression correlated with tumor emboli, metastasis, recurrence and time of recurrence...
March 29, 2018: International Journal of Oncology
Yu Zhang, Shujin He, Renmei Mei, Yurong Kang, Jing Duan, Ran Wei, Chuqi Xiang, Yemeng Wu, Xiangtong Lu, Zhenyu Cai, Lixia Xiong
IL‑13 is a proinflammatory cytokine associated with multiple pathological conditions and the promotion of metastasis in lung cancer. Previous studies have demonstrated that IL‑13 and YY1 are associated with PI3K/AKT signaling. In addition, miR‑29a has been found to play a critical role in cell invasion in lung cancer. However, the molecular mechanism of miR‑29a underlying its involvement in IL‑13‑induced lung cancer cell invasion remains largely unknown. In the present study, we aimed to investigate the role of miR‑29a in cell invasion mediated by IL‑13 in lung cancer...
April 4, 2018: Oncology Reports
Dazhong Yan, Shishan Deng, Weigang Gan, Sijun Li, Yuanquan Li
The current study aimed to identify the effect and primary mechanism of Curcumol on the migration of nasopharyngeal carcinoma (NPC) cells in vitro and in vivo. Curcumol was dissolved in absolute ethyl alcohol and the experiment was performed in NPC 5‑8F cells in vitro and in vivo. The effect of different concentrations of Curcumol on cell migration was determined using wound healing and Transwell assays. A cell counting kit‑8 (CCK‑8) assay was also performed in order to determine cell viability. Flow cytometry was used to detect the effect of Curcumol on apoptosis...
March 28, 2018: Molecular Medicine Reports
Yichun Xu, Hui Yao, Pei Li, Wenbin Xu, Junbin Zhang, Lulu Lv, Haijun Teng, Zhiliang Guo, Huiqing Zhao, Gang Hou
BACKGROUND/AIMS: An adequate matrix production of nucleus pulposus (NP) cells is an important tissue engineering-based strategy to regenerate degenerative discs. Here, we mainly aimed to investigate the effects and mechanism of mechanical compression (i.e., static compression vs. dynamic compression) on the matrix synthesis of three-dimensional (3D) cultured NP cells in vitro. METHODS: Rat NP cells seeded on small intestinal submucosa (SIS) cryogel scaffolds were cultured in the chambers of a self-developed, mechanically active bioreactor for 10 days...
March 26, 2018: Cellular Physiology and Biochemistry
A Mazurkevych, M Malyuk, N Bezdieniezhnykh, L Starodub, Y Kharkevych, A Jakubczak, M Gryzinska
The aim of this study was to establish the immunophenotypic profile and karyotypic stability of bone marrow mesenchymal stem cells (MSCs) of rabbits at the early passages in vitro following the application of different methods of dissociation of cellular material. MSCs were obtained from the femur bone marrow of three clinically healthy rabbits under general anaesthesia. Bone marrow aspirate was seeded in Petri dishes and cultured in a CO2 incubator with 5% CO2 at 37.0oC using a standard procedure. Immunohistochemical detection of nuclear proteins, cytoskeletal proteins and cell adhesion were performed by immunohistochemical analysis and karyotype analysis of MSCs following the enzyme and chelating methods of dissociation of the cell monolayer...
December 2017: Polish Journal of Veterinary Sciences
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