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https://www.readbyqxmd.com/read/29458146/enhanced-anticancer-effects-of-scutellaria-barbata-d-don-in-combination-with-traditional-chinese-medicine-components-on-non-small-cell-lung-cancer-cells
#1
Qian Wang, Narayan Acharya, Zhongwei Liu, Xianmei Zhou, Meghan Cromie, Jia Zhu, Weimin Gao
ETHNOPHARMACOLOGICAL RELEVANCE: Experience-based herbal medicine as a complementary to modern western medicine has triggered an array of studies in quest of novel anticancer drugs. Scutellaria barbata D. Don (SB) is commonly used to treat different types of cancers, but its molecular mechanism of action is not clearly understood. In this study, we attempted to elucidate the mode of action of a traditional Chinese medicine prescription with a total of 14 components, named Lian-Jia-San-Jie-Fang (LJSJF, in Chinese), where SB works as the "principle" against non-small cell lung cancer (NSCLC) cells...
February 16, 2018: Journal of Ethnopharmacology
https://www.readbyqxmd.com/read/29457907/deletion-of-adipose-triglyceride-lipase-links-triacylglycerol-accumulation-to-a-more-aggressive-phenotype-in-a549-lung-carcinoma-cells
#2
Tamara Tomin, Katarina Fritz, Juergen Gindlhuber, Linda Waldherr, Bettina Pucher, Gerhard G Thallinger, Daniel K Nomura, Matthias Schittmayer, Ruth Birner-Gruenberger
Adipose triglyceride lipase (ATGL) catalyzes the rate limiting step in triacylglycerol breakdown in adipocytes but is expressed in most tissues. The enzyme was shown to be lost in many human tumors, and its loss may play a role in early stages of cancer development. Here we report that loss of ATGL supports a more aggressive cancer phenotype in a model system where ATGL was deleted in A549 lung cancer cells by CRISPR/Cas9. We observed that loss of ATGL led to triacylglycerol accumulation in lipid droplets, and higher levels of cellular phospholipid and bioactive lipid species (lyso- and ether-phospholipids)...
February 19, 2018: Journal of Proteome Research
https://www.readbyqxmd.com/read/29455917/respiratory-hazard-assessment-of-combined-exposure-to-complete-gasoline-exhaust-and-respirable-volcanic-ash-in-a-multicellular-human-lung-model-at-the-air-liquid-interface
#3
Ines Tomašek, Claire J Horwell, Christoph Bisig, David E Damby, Pierre Comte, Jan Czerwinski, Alke Petri-Fink, Martin J D Clift, Barbara Drasler, Barbara Rothen-Rutishauser
Communities resident in urban areas located near active volcanoes can experience volcanic ash exposures during, and following, an eruption, in addition to sustained exposures to high concentrations of anthropogenic air pollutants (e.g., vehicle exhaust emissions). Inhalation of anthropogenic pollution is known to cause the onset of, or exacerbate, respiratory and cardiovascular diseases. It is further postulated similar exposure to volcanic ash can also affect such disease states. Understanding of the impact of combined exposure of volcanic ash and anthropogenic pollution to human health, however, remains limited...
February 15, 2018: Environmental Pollution
https://www.readbyqxmd.com/read/29454996/apo-10-lycopenoic-acid-inhibits-cancer-cell-migration-and-angiogenesis-and-induces-peroxisome-proliferator-activated-receptor-%C3%AE
#4
Junrui Cheng, Benchun Miao, Kang-Quan Hu, Xueyan Fu, Xiang-Dong Wang
SCOPE: We have previously shown that apo-10'-lycopenoic acid (ALA), a derivative of lycopene through cleavage by carotene-9',10'-oxygenase, inhibits tumor progression and metastasis in both liver and lung cancer animal models. The underlying mechanism remains unknown. We hypothesized that ALA inhibits cancer cell motility and angiogenesis by up-regulating peroxisome proliferator-activated receptor γ (PPARγ) which is involved in controlling angiogenesis, tumor progression and metastasis...
January 31, 2018: Journal of Nutritional Biochemistry
https://www.readbyqxmd.com/read/29454912/anticancer-effect-of-petroleum-ether-extract-from-bidens-pilosa-l-and-its-constituent-s-analysis-by-gc-ms
#5
Yiwei Shen, Zhouliang Sun, Peiying Shi, Gang Wang, Youjia Wu, Shaoguang Li, Yanjie Zheng, Liying Huang, Liqing Lin, Xinhua Lin, Hong Yao
ETHNOPHARMACOLOGICAL RELEVANCE: Bidens pilosa L, belonging to the family of Acanthaceae, has been used as an anticancer medicine in folk in China. In our preliminary experiments, the petroleum ether extract from B. pilosa showed good cytotoxic activity to human lung cancer A549 cell. However, to date, it's lack of the further study on antitumor effect, mechanism and active substances composition of the petroleum ether extract of B. pilosa. AIM OF THE STUDY: The study aimed to evaluate the anti-lung cancer efficacy of the petroleum ether extract from B...
February 15, 2018: Journal of Ethnopharmacology
https://www.readbyqxmd.com/read/29454903/estrogen-receptor-alpha-isoforms-are-the-main-estrogen-receptors-expressed-in-non-small-cell-lung-carcinoma
#6
Vasiliki Pelekanou, Eleftheria Anastasiou, Efstathia Bakogeorgou, George Notas, Marilena Kampa, Rolando Garcia-Milian, Katerina Lavredaki, Eleni Moustou, Georgia Chinari, Petroula Arapantoni, Anthony O'Grady, Vassilios Georgoulias, Andreas Tsapis, Efstathios N Stathopoulos, Elias Castanas
The expression profile of estrogen receptors (ER) in Non-Small Cell Lung Carcinoma (NSCLC) remains contradictory. Here we investigated protein and transcriptome expression of ERα wild type and variants. Tissue Micro-Arrays of 200 cases of NSCLC (paired tumor/non-tumor) were assayed by immunohistochemistry using a panel of ERα antibodies targeting different epitopes (HC20, 6F11, 1D5, ERα36 and ERα17p). ERβ epitopes were also examined for comparison. In parallel we conducted a probe-set mapping (Affymetrix HGU133 plus 2 chip) meta-analysis of 12 NSCLC tumor public transcriptomic studies (1418 cases) and 39 NSCLC cell lines...
February 15, 2018: Steroids
https://www.readbyqxmd.com/read/29454829/bioactive-triterpenoids-from-twigs-of-betula-schmidtii
#7
Kyoung Jin Park, Lalita Subedi, Sun Yeou Kim, Sang Un Choi, Kang Ro Lee
Investigation of the MeOH extract of Betula schmidtii twigs resulted in the isolation and identification of three new triterpenoids (1-3), along with ten known ones (4-13). The structures of new compounds (1-3) were elucidated by spectroscopic methods, including 1D, 2D NMR (1 H and13 C NMR, COSY, HSQC, HMBC, and NOESY), HR-MS, and chemical methods. All the isolated compounds were evaluated for their cytotoxicity against A549, SK-OV-3, SK-MEL-2, and HCT-15 cell lines. Compound 11 exhibited potent cytotoxic activities against four cell lines, and compounds 5 and 13 significantly induced nerve growth factor secretion in a C6 rat glioma cell line...
February 10, 2018: Bioorganic Chemistry
https://www.readbyqxmd.com/read/29453833/ketamine-induces-apoptosis-in-lung-adenocarcinoma-cells-by-regulating-the-expression-of-cd69
#8
Xuhui Zhou, Peihong Zhang, Wei Luo, Lei Zhang, Rong Hu, Yu Sun, Hong Jiang
Ketamine, an anesthetic, analgesic, or sedative, is widely used for the treatment of cancer pain. Recently, ketamine has been also reported to be tumor repressor for inhibiting proliferation, invasion, and migration, and inducing apoptosis in many cancers. However, whether ketamine can induce the apoptosis of lung adenocarcinoma (LUAD) and which downstream molecular mediates its function remain largely unknown. A LUAD cell line A549 was incubated with ketamine at 0, 1, 10, and 100 μmol/L for 24 h. Trypan blue staining was used to detect the cell viability...
February 17, 2018: Cancer Medicine
https://www.readbyqxmd.com/read/29453377/optimized-peptide-based-inhibitors-targeting-the-dihydrofolate-reductase-pathway-in-cancer
#9
Amrinder Singh, Neha Deshpande, Nilkamal Pramanik, Siddharth Jhunjhunwala, Annapoorni Rangarajan, Hanudatta S Atreya
We report the first peptide based hDHFR inhibitors designed on the basis of structural analysis of dihydrofolate reductase (DHFR). A set of peptides were rationally designed and synthesized using solid phase peptide synthesis and characterized using nuclear magnetic resonance and enzyme immunoassays. The best candidate among them, a tetrapeptide, was chosen based on molecular mechanics calculations and evaluated in human lung adenocarcinoma cell line A549. It showed a significant reduction of cell proliferation and an IC 50 of 82 µM was obtained...
February 16, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29453279/il-12-il-18-cosignaling-in-human-macrophages-and-lung-epithelial-cells-activates-cathelicidin-and-autophagy-inhibiting-intracellular-mycobacterial-growth
#10
Rui Yang, Enzhuo Yang, Ling Shen, Robert L Modlin, Hongbo Shen, Zheng W Chen
The ability of Mycobacterium tuberculosis to block host antimicrobial responses in infected cells provides a key mechanism for disease pathogenesis. The immune system has evolved to overcome this blockade to restrict the infection, but it is not clear whether two key innate cytokines (IL-12/IL-18) involved in host defense can enhance antimycobacterial mechanisms. In this study, we demonstrated that the combination of IL-12 and IL-18 triggered an antimicrobial response against mycobacteria in infected macrophages (THP-1 and human primary monocyte-derived macrophages) and pulmonary epithelial A549 cells...
February 16, 2018: Journal of Immunology: Official Journal of the American Association of Immunologists
https://www.readbyqxmd.com/read/29453076/development-of-novel-synthesized-phthalazinone-based-parp-1-inhibitors-with-apoptosis-inducing-mechanism-in-lung-cancer
#11
Hadia Almahli, Elie Hadchity, Maiy Y Jaballah, Racha Daher, Hazem A Ghabbour, Maha M Kabil, Nasser S Al-Shakliah, Wagdy M Eldehna
Herein we report the synthesis of two series of 4-phenylphthalazin-1-ones 11a-i and 4- benzylphthalazin-1-ones 16a-h as anti-lung adenocarcinoma agents with potential inhibitory activity against PARP-1. All the newly synthesized phthalazinones were evaluated for their anti-proliferative activity against A549 lung carcinoma cell line. Phthalazinones 11c-i and 16b, c showed significant cytotoxic activity against A549 cells at different concentrations (0.1, 1 and 10 μM) for two time intervals (24 h and 48 h)...
February 7, 2018: Bioorganic Chemistry
https://www.readbyqxmd.com/read/29452882/synthesis-characterisation-and-dna-intercalation-studies-of-regioisomers-of-ruthenium-ii-polypyridyl-complexes
#12
Laura Perdisatt, Samar Moqadasi, Luke O'Neill, Gary Hessman, Alessandra Ghion, Muhammad Qasim Mushtaq Warraich, Alan Casey, Christine O'Connor
Regioisomers of the functional group of the main ligand (L) on a series of [Ru(phen) 2 L] 2+ and [Ru(bpy) 2 L] 2+ complexes, where phen is 1,10 phenanthroline and bpy is 2,2'-bipyridine, were synthesised to investigate the interaction with deoxyribonucleic acid (DNA) as potential therapeutics. UV-Vis binding titrations, thermal denaturation and circular dichroism were used to evaluate their interaction with DNA. The conclusions indicated the significance of the auxiliary ligand; especially 1,10-phenanthroline has on the binding constants (K b )...
February 9, 2018: Journal of Inorganic Biochemistry
https://www.readbyqxmd.com/read/29452569/up-regulation-of-sftpb-expression-and-attenuation-of-acute-lung-injury-by-pulmonary-epithelial-cell-specific-nampt-knockdown
#13
Guangliang Bi, Lei Wu, Peixin Huang, Shamima Islam, Daniel P Heruth, Li Qin Zhang, Ding-You Li, Venkatesh Sampath, Weimin Huang, Brett A Simon, Ronald Blaine Easley, Shui Qing Ye
Although a deficiency of surfactant protein B (SFTPB) has been associated with lung injury, SFTPB expression has not yet been linked with nicotinamide phosphoribosyltransferase (NAMPT), a potential biomarker of acute lung injury (ALI). The effects of Nampt in the pulmonary epithelial cell on both SFTPB expression and lung inflammation were investigated in a LPS-induced ALI mouse model. Pulmonary epithelial cell-specific knockdown of Nampt gene expression, achieved by the crossing of Nampt gene exon 2 floxed mice with mice expressing epithelial-specific transgene Cre or by the use of epithelial-specific expression of anti-Nampt antibody cDNA, significantly attenuated LPS-induced ALI...
February 8, 2018: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
https://www.readbyqxmd.com/read/29451710/salvisertin-a-a-new-hexacyclic-triterpenoid-and-other-bioactive-terpenes-from-salvia-deserta-root
#14
Yin-Ru Wang, Yun Yu, Shu-Ming Li, Wei Liu, Wei Li, Susan L Morris-Natschke, Masuo Goto, Kuo-Hsiung Lee, Xue-Feng Huang
Using various chromatographic methods, a new hexacyclic triterpenoid, 2β, 3β, 24β-trihydroxy-12, 13-cyclo-taraxer-l4-en-28oic acid (1), together with ten known compounds, 2α, 3α, 23- trihydroxyurs-12, 20(30)-dien-28oic acid (2), 6, 7-dehydroroyleanone (3), horminone (4), 7-O-methylhorminone (5), sugiol (6), demethylcryptojaponol (7), 14-deoxycoleon U (8), 5, 6-didehydro-7-hydroxy-taxodone (9), ferruginol (10) and dichroanone (11), were isolated from the roots of Salvia deserta. Their structures were identified on the basis of spectroscopic analysis and comparison with the reported data...
February 16, 2018: Chemistry & Biodiversity
https://www.readbyqxmd.com/read/29450740/perfluorocarbon-induces-alveolar-epithelial-cell-response-through-structural-and-mechanical-remodeling
#15
Sofia André Dias, Emmanuelle Planus, Christelle Angely, Luc Lotteau, Renaud Tissier, Marcel Filoche, Bruno Louis, Gabriel Pelle, Daniel Isabey
During total liquid ventilation, lung cells are exposed to perfluorocarbon (PFC) whose chemophysical properties highly differ from standard aqueous cell feeding medium (DMEM). We herein perform a systematic study of structural and mechanical properties of A549 alveolar epithelial cells in order to characterize their response to PFC exposure, using DMEM as control condition. Changes in F-actin structure, focal adhesion density and glycocalyx distribution are evaluated by confocal fluorescent microscopy. Changes in cell mechanics and adhesion are measured by multiscale magnetic twisting cytometry (MTC)...
February 15, 2018: Biomechanics and Modeling in Mechanobiology
https://www.readbyqxmd.com/read/29450633/mid1-pp2a-complex-functions-as-new-insights-in-human-lung-adenocarcinoma
#16
Lin Zhang, Junyao Li, Xuejiao Lv, Tingting Guo, Wei Li, Jie Zhang
PURPOSE: MID1 is an E3 ubiquitin ligase that was first found in Opitz G/BBB syndrome, but there has been little research into its role in lung diseases. We have found an accumulating evidence that indicates the MID1-PP2A complex plays a role in asthma and contributes to inflammation, but its roles in lung adenocarcinoma are unclear. This study aimed at evaluating the function of MID1-PP2A complex in human lung adenocarcinoma. METHODS: We used western blot, ELISA and real-time quantitative PCR to detect the protein and mRNA levels of MID1 and PP2A in A549, H1975, and H1650 lung adenocarcinoma cell lines compared with the human bronchial epithelial cell line BEAS-2B...
February 15, 2018: Journal of Cancer Research and Clinical Oncology
https://www.readbyqxmd.com/read/29449540/s100a4-promotes-lung-tumor-development-through-%C3%AE-catenin-pathway-mediated-autophagy-inhibition
#17
Shasha Hou, Tian Tian, Dianwen Qi, Kaiji Sun, Qi Yuan, Ziling Wang, Zhihai Qin, Zhenlong Wu, Zhinan Chen, Jinhua Zhang
Autophagy has emerged as a critical pathway in tumor development. S100A4 plays important roles in tumor metastasis, but its role in regulating autophagy has not been well characterized. In this study, we found that S100A4 was significantly upregulated in lung adenocarcinoma tissues. Clinical investigation demonstrated that high expression level of S100A4 was associated with tumor size and advanced tumor grades of lung adenocarcinoma patients. Moreover, our results revealed that extracellular S100A4 or overexpression of S100A4 inhibited starvation-induced autophagy and promoted cell proliferation in lung cancer cells in vitro; whereas small interfering RNA (siRNA)-mediated suppression of S100A4 increased autophagy and reduced cell viability in both A549 and LLC cells...
February 15, 2018: Cell Death & Disease
https://www.readbyqxmd.com/read/29448866/multifunctional-mesoporous-silica-nanoparticles-as-efficient-transporters-of-doxorubicin-and-chlorin-e6-for-chemo-photodynamic-combinatorial-cancer-therapy
#18
Jing-Hua Sun, Wei Zhang, Dong-Yang Zhang, Jianliang Shen, Cai-Ping Tan, Liang-Nian Ji, Zong-Wan Mao
A multimodal nanocarrier based on mesoporous silica nanoparticles (MSNs) is developed to co-delivery photosensitizer chlorin e6 (Ce6) and chemotherapeutic agent doxorubicin (Dox) for cancer combination therapy. Ce6 was covalently conjugated with mesoporous silica nanoparticles, which could increase the loading efficiency, and allowed for photodynamic therapy. Doxorubicin was loaded into the pores of mesoporous silica nanoparticles to afford the dual drug delivery system Dox@MSNs-Ce6. These hybrid nanoparticles have an average diameter of about 100 nm and slightly negative charge of about -17 mV...
January 1, 2018: Journal of Biomaterials Applications
https://www.readbyqxmd.com/read/29448627/polysaccharide-functionalized-single-walled-carbon-nanotubes-as-nanocarriers-for-delivery-of-curcumin-in-lung-cancer-cells
#19
Neha Singh, Abhay Sachdev, P Gopinath
Nanoscale drug delivery systems have emerged as promising alternatives to overcome the problems associated with by conventional chemotherapy for cancer treatment such as poor drug stability and bio-distribution. Herein, we report a single walled carbon nanotubes (SWCNTs) based drug delivery system functionalized with polysaccharides such as alginate (ALG) and chitosan (CHI), which can be loaded with an anticancer drug curcumin (CUR). Modification of SWCNTs renders high drug loading efficiency and sustained drug release, imperative for drug activity...
March 1, 2018: Journal of Nanoscience and Nanotechnology
https://www.readbyqxmd.com/read/29448106/folic-acid-prevents-methotrexate-induced-epithelial-mesenchymal-transition-via-suppression-of-secreted-factors-from-the-human-alveolar-epithelial-cell-line-a549
#20
Masashi Kawami, Rika Harabayashi, Risako Harada, Yohei Yamagami, Ryoko Yumoto, Mikihisa Takano
Methotrexate (MTX) often induces serious lung diseases such as pulmonary fibrosis. Although MTX is known to be a folic acid (FA) antagonist, the effect of FA on MTX-induced lung injury remains unclear. Recent studies indicate that epithelial-mesenchymal transition (EMT) is involved in pulmonary fibrosis. Here, we aimed to clarify the effect of FA on MTX-induced EMT in human alveolar epithelial cell line A549 using conditioned medium (CM). CM was prepared from the supernatants of A549 cells treated with MTX in the absence (CMM) or presence (CMMF) of FA...
February 12, 2018: Biochemical and Biophysical Research Communications
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