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Guetchyn Millien, Yuxia Cao, Carl J O'Hara, Jean-Bosco Tagne, Anne Hinds, Mary C Williams, Maria I Ramirez, Hasmeena Kathuria
Distinct members of the Ets family of transcription factors act as positive or negative regulators of genes involved in cellular proliferation, development, and tumorigenesis. In human lung cancer, increased ETS1 expression is associated with poor prognosis and metastasis. We tested whether ETS1 contributes to lung tumorigenesis by binding to Twist1, a gene involved in tumor cell motility and dissemination. We used a mouse lung cancer model with metastasis driven by conditionally activated Kras and concurrent tumor suppressor Lkb1 loss (Kras G12D / Lkb1-/- model) and a similar model of lung cancer that does not metastasize, driven by conditionally activated Kras alone (Kras G12D model)...
June 16, 2018: Clinical & Experimental Metastasis
Ravi Sakthivel, Dicson Sheeja Malar, Kasi Pandima Devi
In the present study, the antiproliferative activity of phytol and its mechanism of action against human lung adenocarcinoma cell line A549 were studied in detail. Results showed that phytol exhibited potent antiproliferative activity against A549 cells in a dose and time-dependent manner with an IC50 value of 70.81 ± 0.32 μM and 60.7 ± 0.47 μM at 24 and 48 h, respectively. Phytol showed no adverse toxic effect in normal human lung cells (L-132), but mild toxic effect was observed when treated with maximum dose (67 and 84 μM)...
June 13, 2018: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
Tingting Lv, Liang Xu, Guolin Wu, Cailong Li, Yibo Wen, Tao Zhang, Haijun Chen, Yu Gao
Chemotherapy is currently one of the promising therapeutic methods for non-small-cell lung cancer (NSCLC), but the emergence of multidrug resistance (MDR) is the greatest obstacle to efficient drug delivery for successful chemotherapy. Nanotechnology-based drug delivery holds great promise to promote intracellular drug delivery to reverse MDR. In this work, we used our previously synthesized ursolic acid (UA) derivative, FZU-03,010 (F3), to prepare nanodrugs of F3 with different architectures and study the role of the structure on the physiochemical properties and the biological effects against A549 and its PTX-resistant A549/PTX lung cancer cells...
June 13, 2018: European Journal of Pharmaceutical Sciences
Foyez Mahmud, Ok-Cheol Jeon, Farzana Alam, Ruby Maharjan, Jeong Uk Choi, Jooho Park, Sojung Lee, Jin Woo Park, Dong Soo Lee, Youngro Byun
There is a growing interest in preclinical research to consider low-dose metronomic chemotherapy as antiangiogenic cancer treatment. Oral metronomic therapy, in particular, has shown much promise with its ease of daily administration and higher therapeutics window. In that regard, we developed oral pemetrexed using the physical complex with the bile acid enhancers (DCK). In a caco-2 permeability study, the oral pemetrexed/DCK complex had significantly higher drug uptake, and inhibited efflux transporter activity as well...
June 13, 2018: Journal of Controlled Release: Official Journal of the Controlled Release Society
Van Hong Nguyen, Nilesh M Meghani, Hardik H Amin, Thao T D Tran, Phuong H L Tran, Chulhun Park, Beom-Jin Lee
Albumin is the most abundant protein in blood, and is the most frequently identified protein in the protein corona of nanoparticles (NPs). Thus, albumin plays an important role in modulating NPs' physicochemical properties and bioavailability. In this study, the effect of bovine serum albumin (BSA) on gelatin-oleic nanoparticles' (GONs) physicochemical properties and cellular uptake were evaluated. Coumarin-6 was used as indicator to track the cellular uptake of GONs. The binding of BSA onto the GON surface increased the size, slightly reduced the negative net charge of the GON, and improved GON stability...
May 28, 2018: Colloids and Surfaces. B, Biointerfaces
Feridun Akkafa, İsmail Koyuncu, Ebru Temiz, Hasan Dagli, Fuat Dïlmec, Halit Akbas
Lung has critic function in gas exchange, supplying oxygen to all cells. Rapid metastasis and the high rate of mortality characterises lung cancer. There are two types of this disease, small cell and non-small cell, which differs from each other according to histopathologic features. To date, many therapeutic approaches have been developed to destroy this deadly type of cancer, which one of them is mRNA targeted therapies through miRNA. miRNAs are 19-25 base paired molecules be able to suppress and destruct mRNA and found to be involved in development and progression of lung cancer...
June 12, 2018: Biochemical and Biophysical Research Communications
Danah S Al-Hattab, Hamza A Safi, Raghu S Nagalingam, Rushita A Bagchi, Matthew Taras Stecy, Michael Paul Czubryt
Numerous physiological and pathological events, from organ development to cancer and fibrosis, are characterized by Epithelial-to-Mesenchymal Transition (EMT), whereby adherent epithelial cells convert to migratory mesenchymal cells. During cardiac development, pro-epicardial organ epithelial cells undergo EMT to generate fibroblasts. Subsequent stress or damage induces further phenotype conversion of fibroblasts to myofibroblasts, causing fibrosis via synthesis of excessive extracellular matrix. We previously showed that the transcription factor scleraxis is both sufficient and necessary for the conversion of cardiac fibroblasts to myofibroblasts, and found that scleraxis knockout reduced cardiac fibroblast number by 50%, possibly via EMT attenuation...
June 15, 2018: American Journal of Physiology. Heart and Circulatory Physiology
Bei Wang, Tze Hau Lam, Mun Kuen Soh, Zhiyong Ye, Jinmiao Chen, Ee Chee Ren
Human influenza virus (IAV) are among the most common pathogens to cause human respiratory infections. A better understanding on interplay between IAV and host factors may provide clues for disease prevention and control. While many viruses are known to downregulate p53 upon entering the cell to reduce the innate host antiviral response, IAV infection is unusual in that it activates p53. However, it has not been clear whether this process has proviral or antiviral effects. In this study, using human isogenic p53 wild-type and p53null A549 cells generated from the CRISPR/Cas9 technology, we observed that p53null cells exhibit significantly reduced viral propagation when infected with influenza A virus (strain A/Puerto Rico/8/1934 H1N1)...
2018: Frontiers in Immunology
Cian-Song Huang, Ai-Chun Huang, Ping-Hsiu Huang, Diana Lo, Yuh-Tai Wang, Ming-Chang Wu
Cisplatin (DPP), a clinically potent antineoplastic agent, is limited by its severe adverse effects. The aim of this study was to investigate the effect of oligogalacturonides (OGA) and DDP on human lung cancer A549 cells. The combined use of OGA and DDP had a synergistic effect on the growth inhibition of A549 cells, changed the cell cycle distribution, and enhanced apoptotic response, especially in sequential combination treatment group of DDP 12 h + OGA 12 h. Western blot analyses showed that the combination treatment of OGA and DDP upregulated Bax, p53, and Caspase-3 and downregulated Bcl-2 proteins...
June 14, 2018: International Journal of Molecular Sciences
Teng Xue, Xiaoyan Xu, Yanqiu Xia, Ya'nan Ren, Fang Zhou
OBJECTIVE: To explore the role of NADPH oxidase 4( NOX4) in transforming growth factor-β( TGF-β)-induced A549 cells migration. METHODS: The A549 cells were allocated into five groups: TGF-β( stimulation) group, Normal control group, DPI( NOX4 inhibitor) group, TGF-β + DPI group, and DMSO( solvent control)group. The level of ROS were detected by flow cytometry instrument. The level of NOX4, snail and E-cadherin protein were detected by western blot. Use scratches experiment to express the change of A549 cells migration...
July 2017: Wei Sheng Yan Jiu, Journal of Hygiene Research
June Lee, Jong-Su Kang, Le Ba Nam, Ok-Kyung Yoo, Young-Sam Keum
NF-E2-related factor 2 (NRF2) regulates transcription of phase II cytoprotective enzymes to protect normal cells against oxidative stress. However, a high level of NRF2 offers a growth advantage, chemoresistance, and radioresistance in cancer. In the present study, we have identified convallatoxin as a novel inhibitor of NRF2/ARE suppression of NRF2 by convallatoxin was not transcriptionally mediated, but regulated at the level of proteolysis. Convallatoxin activated GSK-3β and suppression of NRF2 by convallatoxin required the Neh6 domain...
June 14, 2018: Free Radical Research
Aderonke Ajibola Adeyemo, Abhijith Shettar, Imtiyaz Ahmad Bhat, Paturu Kondaiah, Partha Sarathi Mukherjee
Coordination-driven self-assembly of organometallic η6-arene ruthenium(ii) supramolecular architectures (MA1-MA4) was carried out by employing dinuclear ruthenium acceptors [Ru2(μ-η4-C2O4)(CH3OH)2(η6-p-cymene)2](CF3SO3)2 (Rua), [Ru2(μ-η4-C6H2O4)(CH3OH)2(η6-p-cymene)2](CF3SO3)2 (Rub), [Ru2(dhnq)(H2O)2(η6-p-cymene)2](CF3SO3)2 (Ruc) and [Ru2(dhtq)(H2O)2(η6-p-cymene)2](CF3SO3)2 (Rud) separately with a new tetratopic donor (TD) in methanol at room temperature [TD = N,N,N',N'-tetra(pyridin-4-yl)-[1,1'-biphenyl]-4,4'-diamine]...
June 14, 2018: Dalton Transactions: An International Journal of Inorganic Chemistry
Kanqiu Jiang, Mingjing Shen, Yongbing Chen, Weihua Xu
Lung cancer (LC), as the most common cause of cancer‑related mortality worldwide, is characterized by difficulties in early detection, a high degree of malignancy, poor sensitivity to radiotherapy and chemotherapy, and a low 5‑year survival rate. MicroRNA (miRNA) is a class of small, non‑coding, endogenously expressed RNA that serves vital roles in RNA silencing and post‑transcriptional regulation of gene expression. Previous studies have shown that abnormal expression of miRNA is relevant to various malignant tumors, including lung cancer...
June 12, 2018: Oncology Reports
Fang Li, Tengjiao Zhu, Yao Yue, Xiang Zhu, Jiadong Wang, Li Liang
Non‑small cell lung cancer (NSCLC) is one of the most severe malignant tumor types worldwide. Recent studies have reported an important role of PD‑L1 in mediating immune evasion in the tumor microenvironment. In addition, increasing research has indicated that the expression of PD‑L1 is related to the epithelial‑mesenchymal transition (EMT) process, but the related mechanisms remain to be explored. In the present study, we explored the molecular mechanisms underlying the regulation of PD‑L1 expression during the EMT process in NSCLC cells treated with transforming growth factor‑β1 (TGF‑β1) and fibroblast growth factor 2 (FGF2)...
June 5, 2018: Oncology Reports
Dan Jin, Yan Wu, Cuijie Shao, Yong Gao, Deqiang Wang, Jiwei Guo
Non‑small cell lung cancer (NSCLC) accounts for >80% of all lung cancer cases, which are the leading cause of cancer‑related mortality worldwide. The clinical efficacy of available therapies for NSCLC is often limited due to the development of resistance to anticancer drugs, particularly to cisplatin (DDP). Norcantharidin (NCTD) is a traditional Chinese medicine used in the treatment of many types of cancer, to which patients do not develop resistance. The aim of the present study was to examine the potential synergistic effects of NCTD and DPP on the viability of the the DDP‑resistant NSCLC cell line, A549/DDP...
June 12, 2018: Oncology Reports
Yu Jiang, Gang Liu, Li Zhang, Sheng Cheng, Chun Luo, Yang Liao, Shuliang Guo
The aim of the present study was to investigate the effects of combination therapy of LY294002, a specific inhibitor of phosphatidylinositol 3‑kinase (PI3K), with hydrogen‑rich saline on the proliferation and apoptosis of the non‑small cell lung cancer (NSCLC) A549 cell line and the mechanisms underpinning this. Excessive production of reactive oxygen species (ROS) may induce DNA mutations, DNA damage, genomic instability and cell proliferation, and ROS are involved in several types of cancer, particularly lung cancer...
June 14, 2018: Molecular Medicine Reports
Ying Ji, Jinquan Jiang, Lihua Huang, Wei Feng, Zhang Zhang, Longyu Jin, Xiaowei Xing
Lung cancer is the most common cause of cancer‑related deaths, and early diagnosis and targeted therapy are extremely important in the treatment of this disease. Sperm‑associated antigen 4 (SPAG4) was recently found to be a novel cancer biomarker. In the present study, the expression of SPAG4 was revealed to be high in lung adenocarcinoma tissues as determined by western blotting and immunohistochemistry. SPAG4 knockdown by RNAi efficiently reduced the migration of the lung cancer A549 cells in vitro...
June 4, 2018: Oncology Reports
Arunkumar Naik Bukke, Fathima Nazneen Hadi, K Suresh Babu, P Chandramati Shankar
This article contains data on in vitro cytotoxicity activity of chloroform, methanolic and water extracts of leaf and heartwood of Caesalpinia sappan L. a medicinal plant against Breast cancer (MCF-7) and Lung cancer (A-549) cells. This data shows that Brazilin A, a natural bioactive compound in heartwood of Caesalpinia sappan L. induced cell death in breast cancer (MCF-7) cells. The therapeutic property was further proved by docking the Brazilin A molecule against BCL-2 protein (an apoptotic inhibitor) using auto dock tools...
August 2018: Data in Brief
Xiaoying Ye, Brian T Luke, Bih-Rong Wei, Jan A Kaczmarczyk, Jadranka Loncarek, Jennifer E Dwyer, Donald J Johann, Richard G Saul, Dwight V Nissley, Frank McCormick, Gordon R Whiteley, Josip Blonder
The most widely used cancer animal model is the human-murine tumor xenograft. Unbiased molecular dissection of tumor parenchyma versus stroma in human-murine xenografts is critical for elucidating dysregulated protein networks/pathways and developing therapeutics that may target these two functionally codependent compartments. Although antibody-reliant technologies (e.g., immunohistochemistry, imaging mass cytometry) are capable of distinguishing tumor-proper versus stromal proteins, the breadth or extent of targets is limited...
May 29, 2018: Oncotarget
Desirè Di Silvio, Alessandro Silvestri, Luigi Lay, Laura Polito, Sergio E Moya
Biological fate and toxicity of nanoparticles (NPs) are connected to the interaction between NPs and the protein corona (PC) spontaneously forming around NPs in biological matrixes. PC is a dynamic entity that confers biological identity to NPs. In this work, fluorescence cross-correlation spectroscopy (FCCS) is used to study the impact of specific interactions between the NP surface and proteins on the intracellular fate of PC. The stability of the PC formed around glucosamide-functionalized Au-NPs from ConcanavalinA (ConA) or Bovine Serum Albumin (BSA) is characterized by FCCS...
June 13, 2018: Scientific Reports
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