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https://www.readbyqxmd.com/read/29044153/tripalmitin-nanoparticle-formulations-significantly-enhance-paclitaxel-antitumor-activity-against-breast-and-lung-cancer-cells-in-vitro
#1
María Carmen Leiva, Raúl Ortiz, Rafael Contreras-Cáceres, Gloria Perazzoli, Iryna Mayevych, Juan Manuel López-Romero, Francisco Sarabia, Jose Manuel Baeyens, Consolación Melguizo, Jose Prados
Paclitaxel (PTX) is one of the drugs of choice in the treatment of breast and lung cancer. However, its severe side effects, including mielosuppression, cardiotoxicity and neurotoxicity, frequently cause treatment to be discontinued. Solid lipid nanoparticles (NPs) of glyceril tripalmitate (tripalmitin) loaded with PTX (Tripalm-NPs-PTX) including modifications by the addition of hexa(ethylene glycol), β-cyclodextrin and macelignan were developed. All NPs-PTX formulations displayed excellent hemocompatibility and significantly enhanced PTX antitumor activity in human breast (MCF7, MDAMB231, SKBR3 and T47D) and lung (A549, NCI-H520 and NCI-H460) cancer cells...
October 18, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29043926/efficient-induction-of-apoptosis-in-cancer-cells-by-paclitaxel-loaded-selenium-nanoparticles
#2
Anil Parsram Bidkar, Pallab Sanpui, Siddhartha Sankar Ghosh
AIM: To develop selenium nanoparticles (SeNPs)-based delivery systems for paclitaxel (PTX) and assess their antiproliferative efficacy against cancer cells in vitro with potential mechanistic insight. METHODS: Pluronic F-127 stabilized SeNPs were prepared and characterized. Effects of PTX-loaded SeNPs on lung (A549), breast (MCF7), cervical (HeLa) and colon (HT29) cancer cells were studied by viability assay complemented with flow-cytometric analyses of cell cycle, apoptosis, mitochondrial membrane potential, intracellular reactive oxygen species and caspase activity...
October 18, 2017: Nanomedicine
https://www.readbyqxmd.com/read/29042926/anti-egfr-antibody-conjugated-silica-nanoparticles-as-probes-for-lung-cancer-detection
#3
Jun Wan, Wei Wu, Renquan Zhang, Shandong Liu, Yunlong Huang
A well-designed nanosystem [anti-epidermal growth factor receptor-MB-encapsulated thiol-terminated silica nanoparticles (EGFR/MB-SHSi) complexes] containing silica nanoparticles and near-infrared fluorescence dye (NIRF) methylene blue (MB) was established as a tumor-targeted probe for potential lung cancer detection. The anti-EGFR/MB-SHSi complexes exhibited desirable and homogenous particle size, high bovine serum albumin stability, low hemolytic activity, neutral surface charges and negligible cytotoxicity in vitro...
October 2017: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/29042416/nicotine-prevents-and-reverses-paclitaxel-induced-mechanical-allodynia-in-a-mouse-model-of-cipn
#4
S Lauren Kyte, Wisam Toma, Deniz Bagdas, Julie A Meade, Lesley D Schurman, Aron H Lichtman, Zhi-Jian Chen, Egidio Del Fabbro, Xianjun Fang, John W Bigbee, M Imad Damaj, David A Gewirtz
Chemotherapy-induced peripheral neuropathy (CIPN), a consequence of peripheral nerve fiber dysfunction or degeneration, continues to be a dose-limiting and debilitating side effect during and/or after cancer chemotherapy. Paclitaxel, a taxane commonly used to treat breast, lung, and ovarian cancers, causes CIPN in 59-78% of cancer patients. Novel interventions are needed due to the current lack of effective CIPN treatments. Our studies were designed to investigate if nicotine can prevent and/or reverse paclitaxel-induced peripheral neuropathy in a mouse model of CIPN, while ensuring that nicotine will not stimulate lung tumor cell proliferation or interfere with the anti-tumor properties of paclitaxel...
October 17, 2017: Journal of Pharmacology and Experimental Therapeutics
https://www.readbyqxmd.com/read/29040016/centella-asiatica-fraction-3-suppresses-the-nuclear-factor-erythroid-2-related-factor-2-anti-oxidant-pathway-and-enhances-reactive-oxygen-species-mediated-cell-death-in-cancerous-lung-a549-cells
#5
Dhaneshree Bestinee Naidoo, Alisa Phulukdaree, Krishnan Anand, Vikash Sewram, Anil Amichund Chuturgoon
Centella asiatica is a tropical medicinal plant that is commonly used in traditional medicine. Medicinal properties of C. asiatica include anti-oxidant, anti-inflammatory, and anti-cancer activity. We investigated the anti-oxidant and anti-proliferative/cytotoxic effects of a semi-purified fraction of C. asiatica ethanolic leaf extract (C3) in cancerous lung A549 cells. C3 was obtained by silica column fractionation and identified by using thin-layer chromatography and gas chromatography mass spectrometry. Cytotoxicity of C3 in A549 cells was evaluated (cell viability assay-WST-1; 24 h; [0...
October 2017: Journal of Medicinal Food
https://www.readbyqxmd.com/read/29039734/computational-analysis-of-a-species-d-human-adenovirus-provides-evidence-of-a-novel-virus
#6
Emilio E Espínola, Julio C Barrios, Graciela Russomando, Santiago Mirazo, Juan Arbiza
A human adenovirus (HAdV) species D, was isolated from a hospitalised child with severe lower respiratory infection. It was initially detected in the nasopharyngeal aspirate of the child followed by conventional PCR amplification of the hexon, penton base, and fibre genes. Sanger DNA sequencing and phylogenetic analyses showed characteristics of a recombinant genome not described before. Next Generation Sequencing analysis was performed to reconstruct its complete DNA genome after viral isolation in adenocarcinoma human cell line (A549)...
October 17, 2017: Journal of General Virology
https://www.readbyqxmd.com/read/29039643/exploring-the-ph-sensitivity-of-poly-allylamine-phosphate-supramolecular-nanocarriers-for-intracellular-sirna-delivery
#7
Patrizia Andreozzi, Eleftheria Diamanti, Karen Rapp Py-Daniel, Paolin Rocio Cáceres-Vélez, Chiara Martinelli, Nikolaos Politakos, Ane Escobar, Marco Muzi Falconi, Ricardo Bentes Azevedo, Sergio Moya
Silencing RNA (siRNA) technologies emerge as a promising therapeutic tool for the treatment of multiple diseases. An ideal nanocarrier (NC) for siRNAs should be stable at physiological pH and release siRNAs in acidic endosomal pH, fulfilling siRNA delivery only inside cells. Here, we show a novel application of polyamine phosphate NCs (PANs) based on their capacity to load negatively charged nucleic acids and their pH stability. PANs are fabricated by complexation of phosphate anions from phosphate buffer solution (PB) with the amine groups of poly(allylamine) hydrochloride as carriers for siRNAs...
October 17, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/29039563/inhibition-of-human-lung-cancer-proliferation-through-targeting-stromal-fibroblasts-by-dihydromyricetin
#8
Kai-Jie Fan, Bo Yang, Yang Liu, Xiao-Dong Tian, Bo Wang
In the present study, the effects of dihydromyricetin on the proliferative potential of fibroblasts and lung carcinoma cells were investigated. Markedly higher expression levels of smooth muscle actin and platelet derived growth factors (PDGFs) were observed in the fibroblasts using reverse transcription-polymerase chain reaction analysis. The expression levels of PDGF-A and PDGF-B were also higher in the lung cancer cells. Western blot analysis revealed higher expression levels of the receptor for platelet-derived growth factor (PDGFRβ) in the lysates from fibroblasts obtained from normal tissues and carcinoma tissues...
October 17, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/29039552/prospective-lncrna-mirna-mrna-regulatory-network-of-long-non-coding-rna-linc00968-in-non-small-cell-lung-cancer-a549-cells-a-mirna-microarray-and-bioinformatics-investigation
#9
Dong-Yao Li, Wen-Jie Chen, Lei Luo, Yong-Kun Wang, Jun Shang, Yu Zhang, Gang Chen, Shi-Kang Li
Accumulating evidence suggests that the dysregulation of long non-coding RNAs (lncRNAs) serves vital roles in the incidence and progression of lung cancer. However, the molecular mechanisms of LINC00968, a recently identified lncRNA, remain unknown. The objective of present study was to investigate the role of a prospective lncRNA-miRNA‑mRNA network regulated by LINC00968 in non-small cell lung cancer cells. Following the transfection of lentiviruses carrying LINC00968 into A549 cells, the microRNA (miRNA) expression profile of the cells in response to the overexpression of LINC00968 was detected using an miRNA microarray...
October 12, 2017: International Journal of Molecular Medicine
https://www.readbyqxmd.com/read/29039549/downregulation-of-caspase%C3%A2-3-alleviates-mycoplasma%C3%A2-pneumoniae%C3%A2-induced-apoptosis-in-alveolar-epithelial-cells
#10
Shan Shi, Xiaolei Liu, Haibo Li
Mycoplasma pneumoniae (M. pneumoniae) infection is closely associated with pneumonia in children. Apoptosis of alveolar epithelial cells is involved in the development of pneumonia in children. The present study aimed to examine how caspase‑3 influences apoptosis rates in M. pneumoniae‑infected alveolar epithelial cells. A549 alveolar epithelial cells were treated with M. pneumoniae, and cells and culture supernatant were collected at different time points. Alterations in apoptosis rates and caspase‑3 mRNA and protein levels were measured for each treatment group...
October 12, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/29039479/mdig-suppresses-epithelial-mesenchymal-transition-and-inhibits-the-invasion-and-metastasis-of-non%C3%A2-small-cell-lung-cancer-via-regulating-gsk-3%C3%AE-%C3%AE-catenin-signaling
#11
Feng Geng, Zhanshi Jiang, Xiaogang Song, Haomin Zhou, Hongwen Zhao
Mineral dust-induced gene (mdig) can inhibit the invasion and metastasis of A549 cells. The main purpose of this study was to explore the molecular mechanism underlying the inhibitory effect of mdig on cell invasion and metastasis. Mdig-knockdown and mdig-overexpressing A549 cells and an mdig-overexpressing human umbilical vein endothelial cell (HUVEC) line were constructed using lentiviral vectors, and western blot analysis was performed to verify the silencing and overexpression of the mdig protein. A Transwell invasion assay was used to detect the invasive abilities of each experimental group, and Transwell migration and scratch assays were used to detect cell migration ability...
October 12, 2017: International Journal of Oncology
https://www.readbyqxmd.com/read/29037838/expression-and-epigenetic-regulation-of-cystatin-b-in-lung-cancer-and-colorectal-cancer
#12
Yunxia Ma, Yuan Chen, Iver Petersen
AIMS: Dysregulated expression of cystatin B (CSTB) has been implicated in various cancers. The aims of this study were to analyze the CSTB expression and investigate the epigenetic regulation of CSTB in lung and colon cancer cell lines, and also evaluate the clinical outcome of CSTB in primary lung and colorectal tumors. METHODS: CSTB expression in lung and colon cancer cell lines was analyzed by real-time RT-PCR and western blotting. Epigenetic regulation of CSTB was examined by demethylation, deacetylation tests and bisulfite sequencing (BS)...
June 6, 2017: Pathology, Research and Practice
https://www.readbyqxmd.com/read/29035746/the-exploration-of-endocytic-mechanisms-of-pla-peg-nanoparticles-prepared-by-coaxialtri-capillary-electrospray-template-removal-method
#13
Jiaming Chen, Lihua Cao, Yuecheng Cui, Kehua Tu, Hongjun Wang, Li-Qun Wang
The nano-sized poly(lactic acid)-poly(ethylene glycol) (PLA-PEG) particles with core-shell structure were efficiently prepared by using coaxial tri-capillary electrospray-template removal method. The cellular uptake mechanism, intracellular distribution and exocytosis in A549 cell model of electrosprayed PLA-PEG nanoparticles were systemically studied. The drug release behavior of electrosprayed PLA-PEG nanoparticles were also investigated. Our results showed that PLA-PEG nanoparticles can be endocytosed quickly by A549 cells...
October 2, 2017: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/29035560/cytotoxic-dibohemamines-d-f-from-a-streptomyces-species
#14
Bingya Jiang, Wei Zhao, Shufen Li, Hongyu Liu, Liyan Yu, Yixuan Zhang, Hongwei He, Linzhuan Wu
Three dimeric analogues of bohemamines, dibohemamines D-F (1-3), together with dibohemamine A (4), were isolated from Streptomyces sp. CPCC 200497. Their structures were solved using a combination of mass spectrometry, 1D and 2D NMR spectroscopy, and CD. Dibohemamines D and E were new dimeric analogues of bohemamines, and dibohemamine F was a known compound obtained previously by semisynthesis. Dibohemamine F displayed potent cytotoxicity against cancer cell lines A549 and HepG2 with IC50 values of 1.1 and 0...
October 16, 2017: Journal of Natural Products
https://www.readbyqxmd.com/read/29035132/hybrid-lipid-polymer-nanoparticles-for-pulmonary-delivery-of-sirna-development-and-fate-upon-in-vitro-deposition-on-the-human-epithelial-airway-barrier
#15
Ivana d'Angelo, Gabriella Costabile, Estelle Durantie, Paola Brocca, Valeria Rondelli, Annapina Russo, Giulia Russo, Agnese Miro, Fabiana Quaglia, Alke Petri-Fink, Barbara Rothen-Rutishauser, Francesca Ungaro
BACKGROUND: Nowadays, the downregulation of genes involved in the pathogenesis of severe lung diseases through local siRNA delivery appears an interesting therapeutic approach. In this study, we propose novel hybrid lipid-polymer nanoparticles (hNPs) consisting of poly(lactic-co-glycolic) acid (PLGA) and dipalmitoyl phosphatidylcholine (DPPC) as siRNA inhalation system. METHODS: A panel of DPPC/PLGA hNPs was prepared by emulsion/solvent diffusion and fully characterized...
October 16, 2017: Journal of Aerosol Medicine and Pulmonary Drug Delivery
https://www.readbyqxmd.com/read/29034629/-comparison-of-cellular-localization-of-ns1-from-different-subtypes-of-influenza-a-virus
#16
Meng Yu, Shuaishuai Cao, Weinan Zheng, Xiaoxiao Jia, Jing Li, Wenjun Liu
The non-structural (NS1) protein is a multifunctional molecular protein encoded by influenza A virus genome. NS1 plays an important role in inhibition of host immune responses. In order to assess the cellular localization of NS1 in different influenza A virus subtypes, we performed the immunofluorescence assay to observe the cellular location of NS1 after infection with influenza A virus WSN (H1N1), PR8 (H1N1), CA04 (H1N1), SD (H9N2) and AH01 (H7N9) in A549 cells and MDCK cells respectively. According to the results, NS1-WSN and NS1-PR8 accumulated mainly in cytoplasm at 24 h post infection, while the NS1-CA04 and NS1-SD appeared major in the nucleus...
November 25, 2016: Sheng Wu Gong Cheng Xue Bao, Chinese Journal of Biotechnology
https://www.readbyqxmd.com/read/29032986/mycobacterium-tuberculosis-strains-induce-strain-specific-cytokine-and-chemokine-response-in-pulmonary-epithelial-cells
#17
Nontobeko E Mvubu, Balakrishna Pillay, Lyle R McKinnon, Manormoney Pillay
M. tuberculosis F15/LAM4/KZN has been associated with high transmission rates of drug resistant tuberculosis in the KwaZulu-Natal province of South Africa. The current study elucidated the cytokine/chemokine responses induced by representatives of the F15/LAM4/KZN and other dominant strain families in pulmonary epithelial cells. Multiplex cytokine analyses were performed at 24, 48 and 72h post infection of the A549 pulmonary epithelial cell line with the F15/LAM4/KZN, F28, F11, Beijing, Unique and H37Rv strains at an MOI of ∼10:1...
October 9, 2017: Cytokine
https://www.readbyqxmd.com/read/29032339/synthesis-and-evaluation-of-anticancer-activity-of-boc26p-an-ortho-aryl-chalcone-sodium-phosphate-as-water-soluble-prodrugs-in-vitro-and-in-vivo
#18
Cuige Zhu, Ruimin Wang, Weichao Zheng, Daoyuan Chen, Xin Yue, Yingnan Cao, Wenjing Qin, Haixia Sun, Youqiao Wang, Ziyi Liu, Baojian Li, Jun Du, Xianzhang Bu, Binhua Zhou
Major limitations of chalcones as clinical anticancer agents are water insolubility and poor bioavailability, which may be improved by a classic phosphate prodrug strategy that targets non-specific alkaline phosphatase (ALP) for releasing the parent drug in vivo. In this study, we found that BOC26P, a phosphate prodrug of chalcone OC26, exhibits excellent water solubility and improved plasma concentration in vivo by either i.v. or p.o. compared with the parent drug. In pace with decreased inhibitory activity of BOC26P against microtubule polymerization in vitro and in cells, the antiproliferative activity of BOC26P is attenuated in A549 and HLF cells...
October 12, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/29032193/selenium-functionalized-liposomes-for-systemic-delivery-of-doxorubicin-with-enhanced-pharmacokinetics-and-anticancer-effect
#19
Qian Xie, Wenji Deng, Xue Yuan, Huan Wang, Zhiguo Ma, Baojian Wu, Xingwang Zhang
Liposomes have shown to be an excellent drug delivery system, but the short in vivo fate discourages their popularity. This work aimed to develop selenium-functionalized liposomes (SeLPs) for doxorubicin (Dox) delivery to prolong the systemic circulation of liposomes by in situ selenium coating and enhance the anticancer effect via the synergy between Dox and selenium. Dox-loaded SeLPs (Dox-SeLPs) were prepared by film hydration/active loading/in situ reduction technique and characterized by particle size, entrapment efficiency and morphology...
October 12, 2017: European Journal of Pharmaceutics and Biopharmaceutics
https://www.readbyqxmd.com/read/29031922/catechin-loaded-plga-submicron-sized-fibers-reduce-levels-of-reactive-oxygen-species-induced-by-mwcnt-in-vitro
#20
Roxana E Ghitescu, Ana Maria Popa, Angela Schipanski, Cordula Hirsch, Gökce Yazgan, Valentin I Popa, René M Rossi, Katharina Maniura-Weber, Giuseppino Fortunato
Reactive oxygen species (ROS) are common products of normal aerobic cellular metabolism, but high levels of ROS lead to oxidative stress and cellular damage. Therefore, effective antioxidant therapies are needed to prevent ROS overproduction. This study reports the development of poly(l-lactide-co-glycolide) (PLGA) bicomponent fibers loaded with selected amounts of the natural polyphenolic antioxidant catechin. Thereby a novel route based on emulsion electrospinning is investigated to obtain tailored and sustained release rates for chatechin...
October 11, 2017: European Journal of Pharmaceutics and Biopharmaceutics
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