Sineadh M Conway, Chao-Cheng Kuo, Woodrow Gardiner, Rui-Ni Wu, Loc V Thang, Graydon B Gereau, John R Cirrito, Carla M Yuede, Jordan G McCall, Ream Al-Hasani
The endogenous opioid peptide systems are critical for analgesia, reward processing, and affect, but research on their release dynamics and function has been challenging. Here, we have developed microimmunoelectrodes (MIEs) for the electrochemical detection of opioid peptides using square-wave voltammetry. Briefly, a voltage is applied to the electrode to cause oxidation of the tyrosine residue on the opioid peptide of interest, which is detected as current. To provide selectivity to these voltammetric measurements, the carbon fiber surface of the MIE is coated with an antiserum selective to the opioid peptide of interest...
February 3, 2023: bioRxiv