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met-enkephalin

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https://www.readbyqxmd.com/read/27916729/enkephalin-and-neuropeptide-y-interaction-in-the-intergeniculate-leaflet-network-a-part-of-the-mammalian-biological-clock
#1
K Palus, L Chrobok, M Kepczynski, M H Lewandowski
The intergeniculate leaflet (IGL) is a flat thalamic nucleus implicated in the modulation of circadian rhythmicity. In rat, two main GABAergic subpopulations can be distinguished in the IGL: neurons synthesizing neuropeptide Y (NPY), which directly innervates the suprachiasmatic nuclei, and enkephalinergic cells, which connect contralaterally located leaflets. The aim of this study was to evaluate possible effects of inner IGL neurotransmitters on the spontaneous and synaptic activity of IGL neurons. The data presented in this article provide evidence that enkephalin, and not NPY, could act upon the majority of IGL neurons...
December 2, 2016: Neuroscience
https://www.readbyqxmd.com/read/27825215/comparing-generalized-ensemble-methods-for-sampling-of-systems-with-many-degrees-of-freedom
#2
James Lincoff, Sukanya Sasmal, Teresa Head-Gordon
We compare two standard replica exchange methods using temperature and dielectric constant as the scaling variables for independent replicas against two new corresponding enhanced sampling methods based on non-equilibrium statistical cooling (temperature) or descreening (dielectric). We test the four methods on a rough 1D potential as well as for alanine dipeptide in water, for which their relatively small phase space allows for the ability to define quantitative convergence metrics. We show that both dielectric methods are inferior to the temperature enhanced sampling methods, and in turn show that temperature cool walking (TCW) systematically outperforms the standard temperature replica exchange (TREx) method...
November 7, 2016: Journal of Chemical Physics
https://www.readbyqxmd.com/read/27759693/giant-basal-cell-carcinomas-express-neuroactive-mediators-and-show-a-high-growth-rate-a-case-control-study-and-meta-analysis-of-etiopathogenic-and-prognostic-factors
#3
Mohammad-Ali Yazdani Abyaneh, Peter Engel, Andrzej Slominski, Bruce Ragsdale, Richard Agag, Daniel Cramer, J Andrew Carlson
BACKGROUND: Giant basal cell carcinomas (GBCCs), (BCC ≥ 5 cm), are often painless, destructive tumors resulting from poorly understood patient neglect. OBJECTIVES: To elucidate etiopathogenic factors distinguishing GBCC from basal cell carcinoma (BCC) and identify predictors for disease-specific death (DSD). METHODS: Case-control study examining clinicopathologic and neuroactive factors (β-endorphin, met-enkephalin, serotonin, adrenocorticotropic hormone, and neurofilament expression) in GBCC and BCC...
October 18, 2016: American Journal of Dermatopathology
https://www.readbyqxmd.com/read/27744679/identification-of-the-first-marine-derived-opioid-receptor-balanced-agonist-with-a-signaling-profile-that-resembles-the-endorphins
#4
Tyler A Johnson, Laura Milan-Lobo, Tao Che, Madeline Ferwerda, Eptisam Lambo, Nicole L McIntosh, Fei Li, Li He, Nicholas Lorig-Roach, Phillip Crews, Jennifer Lynne Whistler
Opioid therapeutics are excellent analgesics, whose utility is compromised by dependence. Morphine (1) and its clinically relevant derivatives such as OxyContin® (4), Vicodin® (5) and Dilaudid® (6) are "biased" agonists at the µ opioid receptor (OR), wherein they engage G-protein signaling but poorly engage β-arrestin and the endocytic machinery. In contrast, the endorphins, met-enkephalin (14) and β-endorphin (15), endogenous peptide agonists for ORs, are more potent analgesics then 1, show reduced liability for tolerance and dependence, and engage both G-protein and β-arrestin pathways as "balanced" agonists...
October 17, 2016: ACS Chemical Neuroscience
https://www.readbyqxmd.com/read/27717401/adoptive-transfer-of-m2-macrophages-reduces-neuropathic-pain-via-opioid-peptides
#5
Maria Pannell, Dominika Labuz, Melih Ö Celik, Jacqueline Keye, Arvind Batra, Britta Siegmund, Halina Machelska
BACKGROUND: During the inflammation which occurs following nerve damage, macrophages are recruited to the site of injury. Phenotypic diversity is a hallmark of the macrophage lineage and includes pro-inflammatory M1 and anti-inflammatory M2 populations. Our aim in this study was to investigate the ability of polarized M0, M1, and M2 macrophages to secrete opioid peptides and to examine their relative contribution to the modulation of neuropathic pain. METHODS: Mouse bone marrow-derived cells were cultured as unstimulated M0 macrophages or were stimulated into an M1 phenotype using lipopolysaccharide and interferon-γ or into an M2 phenotype using interleukin-4...
October 7, 2016: Journal of Neuroinflammation
https://www.readbyqxmd.com/read/27605249/distinct-roles-of-exogenous-opioid-agonists-and-endogenous-opioid-peptides-in-the-peripheral-control-of-neuropathy-triggered-heat-pain
#6
Dominika Labuz, Melih Ö Celik, Andreas Zimmer, Halina Machelska
Neuropathic pain often results from peripheral nerve damage, which can involve immune response. Local leukocyte-derived opioid peptides or exogenous opioid agonists inhibit neuropathy-induced mechanical hypersensitivity in animal models. Since neuropathic pain can also be augmented by heat, in this study we investigated the role of opioids in the modulation of neuropathy-evoked heat hypersensitivity. We used a chronic constriction injury of the sciatic nerve in wild-type and opioid peptide-knockout mice, and tested opioid effects in heat and mechanical hypersensitivity using Hargreaves and von Frey tests, respectively...
2016: Scientific Reports
https://www.readbyqxmd.com/read/27543451/histologic-and-immunohistochemical-characterization-of-pheochromocytomas-in-20-clouded-leopards-neofelis-nebulosa
#7
S Corner, T Walsh, L Padilla, A MacNeill, M Wallig, M Kiupel, K Terio
Clouded leopards in North American zoological institutions have a high frequency of pheochromocytomas and were identified in 32 of 70 (45%) animals necropsied. Archival sections of adrenal gland from 20 adult clouded leopards with unilateral or bilateral pheochromocytomas collected between 1984 and 2011 were examined by light microscopy and immunohistochemistry, and case demographics were reviewed. Affected leopards were older than 10 years of age (mean, 16 years; range, 11-19 years), and males were overrepresented (12 males, 8 females)...
August 19, 2016: Veterinary Pathology
https://www.readbyqxmd.com/read/27498137/%C3%AE-opioid-receptors-pivotal-role-in-intermittent-hypoxia-augmentation-of-cardiac-parasympathetic-control-and-plasticity
#8
Juan A Estrada, Mathew A Barlow, Darice Yoshishige, Arthur G Williams, H Fred Downey, Robert T Mallet, James L Caffrey
BACKGROUND: Intermittent hypoxia training (IHT) produces robust myocardial protection against ischemia-reperfusion induced infarction and arrhythmias. Blockade of this cardioprotection by antagonism of either β1-adrenergic or δ-opioid receptors (δ-OR) suggests autonomic and/or opioidergic adaptations. PURPOSE: To test the hypothesis that IHT shifts cardiac autonomic balance toward greater cholinergic and opioidergic influence. METHODS: Mongrel dogs completed 20d IHT, non-hypoxic sham training, or IHT with the δ-OR antagonist naltrindole (200μg/kgsc)...
July 2016: Autonomic Neuroscience: Basic & Clinical
https://www.readbyqxmd.com/read/27434150/synthesis-of-met-enkephalin-by-solution-phase-peptide-synthesis-methodology-utilizing-para-toluene-sulfonic-acid-as-n-terminal-masking-of-l-methionine-amino-acid
#9
Riaz A Khan
The Met-enkephalin, Tyr-Gly-Gly-Phe-Met, was synthesized by the solution phase synthesis (SPS) methodology employing -OBzl group as carboxyls protection while the t-Boc groups were employed for the N-terminal α-amines protection for the majority of the amino acids of the pentapeptide sequence. The L-methionine (L-Met) amino acid was used as PTSA.Met-OBzl obtained from the simultaneous protection of the α-amino, and carboxyl group with para-toluene sulfonic acid (PTSA) and as benzyl alcohol ester respectively, in a C-terminal start of the 2+2+1 fragments condensation convergent synthetic approach...
July 19, 2016: Chemical Biology & Drug Design
https://www.readbyqxmd.com/read/27409457/smart-polymeric-nanocarriers-of-met-enkephalin
#10
Roza Szweda, Barbara Trzebicka, Andrzej Dworak, Lukasz Otulakowski, Dominik Kosowski, Justyna Hertlein, Emi Haladjova, Stanislav Rangelov, Dawid Szweda
This study describes a novel approach to polymeric nanocarriers of the therapeutic peptide met-enkephalin based on the aggregation of thermoresponsive polymers. Thermoresponsive bioconjugate poly((di(ethylene glycol) monomethyl ether methacrylate)-ran-(oligo(ethylene glycol) monomethyl ether methacrylate) is synthesized by AGET ATRP using modified met-enkephalin as a macroinitiator. The abrupt heating of bioconjugate water solution leads to the self-assembly of bioconjugate chains and the formation of mesoglobules of controlled sizes...
August 8, 2016: Biomacromolecules
https://www.readbyqxmd.com/read/27194431/pheochromocytoma-in-old-world-primates-macaca-mulatta-and-chlorocebus-aethiops
#11
L M A Colgin, D J Schwahn, F Castillo-Alcala, M Kiupel, A D Lewis
Pheochromocytoma, a rarely reported adrenal gland neoplasm in Old World primates, was diagnosed in 5 rhesus macaques (Macaca mulatta) and 2 African green monkeys (Chlorocebus aethiops) from 3 research institutions. Age and sex were available for 6 primates. Two males and 4 females were affected, ranging in age from 9 to 31 years. All neoplasms were unilateral and, in the cases reporting the affected gland, 4 involved the right adrenal gland and 2 involved the left. Diagnosis was established by characteristic histologic features...
November 2016: Veterinary Pathology
https://www.readbyqxmd.com/read/27194343/dopamine-d1-like-receptors-regulate-constitutive-%C3%AE-opioid-receptor-mediated-repression-of-use-dependent-synaptic-plasticity-in-dorsal-horn-neurons-more-harm-than-good
#12
Zigor Aira, Teresa Barrenetxea, Itsaso Buesa, Gontzal García Del Caño, Jon Jatsu Azkue
UNLABELLED: The current study reports on a synaptic mechanism through which D1-like receptors (D1LRs) modulate spinal nociception and plasticity by regulating activation of the μ-opioid receptor (MOR).D1LR stimulation with agonist SKF 38393 concentration-dependently depressed C-fiber-evoked potentials in rats receiving spinal nerve ligation (SNL), but not in uninjured rats. Depression was prevented by MOR- but not GABA-receptor blockade. Neurons expressing the D1 subtype were immunopositive for met-enkephalin and vesicular glutamate transporter VGLUT2, but not for GABAergic marker vGAT...
May 18, 2016: Journal of Neuroscience: the Official Journal of the Society for Neuroscience
https://www.readbyqxmd.com/read/27139929/leukocyte-opioid-receptors-mediate-analgesia-via-ca-2-regulated-release-of-opioid-peptides
#13
Melih Ö Celik, Dominika Labuz, Karen Henning, Melanie Busch-Dienstfertig, Claire Gaveriaux-Ruff, Brigitte L Kieffer, Andreas Zimmer, Halina Machelska
Opioids are the most powerful analgesics. As pain is driven by sensory transmission and opioid receptors couple to inhibitory G proteins, according to the classical concept, opioids alleviate pain by activating receptors on neurons and blocking the release of excitatory mediators (e.g., substance P). Here we show that analgesia can be mediated by opioid receptors in immune cells. We propose that activation of leukocyte opioid receptors leads to the secretion of opioid peptides Met-enkephalin, β-endorphin and dynorphin A (1-17), which subsequently act at local neuronal receptors, to relieve pain...
October 2016: Brain, Behavior, and Immunity
https://www.readbyqxmd.com/read/27070761/taboo-search-algorithm-with-a-modified-inverse-histogram-for-reproducing-biologically-relevant-rare-events-of-proteins
#14
Ryuhei Harada, Yu Takano, Yasuteru Shigeta
The TaBoo SeArch (TBSA) algorithm [ Harada et al. J. Comput. Chem. 2015 , 36 , 763 - 772 and Harada et al. Chem. Phys. Lett. 2015 , 630 , 68 - 75 ] was recently proposed as an enhanced conformational sampling method for reproducing biologically relevant rare events of a given protein. In TBSA, an inverse histogram of the original distribution, mapped onto a set of reaction coordinates, is constructed from trajectories obtained by multiple short-time molecular dynamics (MD) simulations. Rarely occurring states of a given protein are statistically selected as new initial states based on the inverse histogram, and resampling is performed by restarting the MD simulations from the new initial states to promote the conformational transition...
May 10, 2016: Journal of Chemical Theory and Computation
https://www.readbyqxmd.com/read/27007059/minimizing-postsampling-degradation-of-peptides-by-a-thermal-benchtop-tissue-stabilization-method
#15
Lova Segerström, Jenny Gustavsson, Ingrid Nylander
Enzymatic degradation is a major concern in peptide analysis. Postmortem metabolism in biological samples entails considerable risk for measurements misrepresentative of true in vivo concentrations. It is therefore vital to find reliable, reproducible, and easy-to-use procedures to inhibit enzymatic activity in fresh tissues before subjecting them to qualitative and quantitative analyses. The aim of this study was to test a benchtop thermal stabilization method to optimize measurement of endogenous opioids in brain tissue...
April 2016: Biopreservation and Biobanking
https://www.readbyqxmd.com/read/26835559/light-and-electron-microscopic-analysis-of-enkephalin-like-immunoreactivity-in-the-basolateral-amygdala-including-evidence-for-convergence-of-enkephalin-containing-axon-terminals-and-norepinephrine-transporter-containing-axon-terminals-onto-common-targets
#16
Jingyi Zhang, Alexander J McDonald
Modulatory interactions of opioids and norepinephrine (NE) in the anterior subdivision of the basolateral nucleus of the amygdala (BLa) are critical for the consolidation of memories of emotionally arousing experiences. Although there have been several studies of the noradrenergic system in the amygdalar basolateral nuclear complex (BLC), little is known about the chemical neuroanatomy of opioid systems in this region. To address this knowledge gap the present study first examined the distribution of met-enkephalin-like immunoreactivity (ENK-ir) in the BLC at the light microscopic level, and then utilized dual-labeling immunocytochemistry combined with electron microscopy to investigate the extent of convergence of NE and ENK terminals onto common structures in the BLa...
April 1, 2016: Brain Research
https://www.readbyqxmd.com/read/26802437/npyfa-a-chimeric-peptide-of-met-enkephalin-and-npff-induces-tolerance-free-analgesia
#17
Annu Mudgal, Krishan Kumar, Catherine Mollereau, Santosh Pasha
Methionine-enkephalin-Arg-Phe is an endogenous amphiactive analgesic peptide. Neuropeptide FF, on the other hand, is reported for its role in opioid modulation and tolerance development. Based on these reports, in the present study we designed a chimeric peptide NPYFa (YGGFMKKKPQRFamide), having the Met-enkephalin (opioid) and PQRFamide sequence of neuropeptide FF, which can then target both the opioid and neuropeptide FF receptors. We hypothesized that the chimeric peptide so designed would have both analgesic properties and further aid in understanding of the role of neuropeptide FF in the development of opiate tolerance...
June 2016: Chemical Biology & Drug Design
https://www.readbyqxmd.com/read/26721148/-anti-inflammatory-and-synovial-opioid-system-effects-of-electroacupuncture-intervention-on-chronic-pain-in-arthritic-rats
#18
Yongliang Jiang, Xiaofen He, Xiaohu Yin, Yafang Shen, Jianqiao Fang
OBJECTIVE: To observe the analgesic effect of electroacupuncture (EA) on collagen-induced arthritis (CIA) rats and its regulating effect on inflammation reaction and the endogenous opioid system of synovial tissues. Methods A total of 30 healthy male Wistar rats were randomly divided into a control group, a model group and an EA group, 10 rats in each one. The chronic pain model of CIA rats was made by cattle type-II collagen in the model group and EA group. Rats in the EA group were treated with EA at "Zusanli" (ST 36) and "Kunlun" (BL 60) for 30 min from 16th day after model establishment, once a day for consecutive 10 days...
September 2015: Zhongguo Zhen Jiu, Chinese Acupuncture & Moxibustion
https://www.readbyqxmd.com/read/26663709/the-opioid-peptide-beta-endorphin-stimulates-acrosome-reaction-in-human-spermatozoa
#19
I Urizar-Arenaza, H Estomba, I Muñoa-Hoyos, R Matorras, A Esposito, L Candenas, F M Pinto, A Valdivia, J Irazusta, N Subirán
The acrosome reaction occurs in vivo following sperm capacitation and is essential for the acquisition of sperm fertilization ability. However, little is known about the molecular identity of the physiological acrosome reaction regulators. In addition to progesterone, which is produced by cumulus oophorus cells and known to regulate acrosome reaction by activating the specific calcium channel CatSper, endogenous opioid peptides such as beta-endorphin and met-enkephalin are present at high concentrations in the follicular fluid suggesting that the opioid system may be involved in the mechanisms regulating the acrosome reaction in humans...
January 2016: Andrology
https://www.readbyqxmd.com/read/26634308/endogenous-opioids-contribute-to-insensitivity-to-pain-in-humans-and-mice-lacking-sodium-channel-nav1-7
#20
Michael S Minett, Vanessa Pereira, Shafaq Sikandar, Ayako Matsuyama, Stéphane Lolignier, Alexandros H Kanellopoulos, Flavia Mancini, Gian D Iannetti, Yury D Bogdanov, Sonia Santana-Varela, Queensta Millet, Giorgios Baskozos, Raymond MacAllister, James J Cox, Jing Zhao, John N Wood
Loss-of-function mutations in the SCN9A gene encoding voltage-gated sodium channel Nav1.7 cause congenital insensitivity to pain in humans and mice. Surprisingly, many potent selective antagonists of Nav1.7 are weak analgesics. We investigated whether Nav1.7, as well as contributing to electrical signalling, may have additional functions. Here we report that Nav1.7 deletion has profound effects on gene expression, leading to an upregulation of enkephalin precursor Penk mRNA and met-enkephalin protein in sensory neurons...
December 4, 2015: Nature Communications
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