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https://www.readbyqxmd.com/read/28088490/microwave-based-treatments-of-wheat-kernels-do-not-abolish-gluten-epitopes-implicated-in-celiac-disease
#1
Carmen Gianfrani, Gianfranco Mamone, Barbara la Gatta, Alessandra Camarca, Luigia Di Stasio, Francesco Maurano, Stefania Picascia, Vito Capozzi, Giuseppe Perna, Gianluca Picariello, Aldo Di Luccia
Microwave based treatment (MWT) of wet wheat kernels induced a striking reduction of gluten, up to <20 ppm as determined by R5-antibodybased ELISA, so that wheat could be labeled as gluten-free. In contrast, analysis of gluten peptides by G12 antibody-based ELISA, mass spectrometry-based proteomics and in vitro assay with T cells of celiac subjects, indicated no difference of antigenicity before and after MWT. SDS-PAGE analysis and Raman spectroscopy demonstrated that MWT simply induced conformational modifications, reducing alcohol solubility of gliadins and altering the access of R5-antibody to the gluten epitopes...
January 11, 2017: Food and Chemical Toxicology
https://www.readbyqxmd.com/read/28086802/brain-metabolic-pattern-analysis-using-a-magnetic-resonance-spectra-classification-software-in-experimental-stroke
#2
Elena Jiménez-Xarrié, Myriam Davila, Ana Paula Candiota, Raquel Delgado-Mederos, Sandra Ortega-Martorell, Margarida Julià-Sapé, Carles Arús, Joan Martí-Fàbregas
BACKGROUND: Magnetic resonance spectroscopy (MRS) provides non-invasive information about the metabolic pattern of the brain parenchyma in vivo. The SpectraClassifier software performs MRS pattern-recognition by determining the spectral features (metabolites) which can be used objectively to classify spectra. Our aim was to develop an Infarct Evolution Classifier and a Brain Regions Classifier in a rat model of focal ischemic stroke using SpectraClassifier. RESULTS: A total of 164 single-voxel proton spectra obtained with a 7 Tesla magnet at an echo time of 12 ms from non-infarcted parenchyma, subventricular zones and infarcted parenchyma were analyzed with SpectraClassifier ( http://gabrmn...
January 13, 2017: BMC Neuroscience
https://www.readbyqxmd.com/read/28081308/exhaled-carbon-monoxide-is-a-marker-of-heavy-nicotine-dependence
#3
Elif Babaoğlu, Ayşegül Karalezli, Mükremin Er, Hatice Canan Hasanoğlu, Derya Öztuna
BACKGROUND/AIM: Exhaled CO level provides an objective measure of a patient's smoking status. The relationship between CO levels and nicotine dependence is controversial. The aim of this study is to evaluate the relationship between exhaled CO levels and nicotine dependence as well as to demonstrate that exhaled CO levels may be used as a marker of nicotine dependence. MATERIALS AND METHODS: Two hundred eighty-nine patients (132 females, 157 males) were included in the study...
December 20, 2016: Turkish Journal of Medical Sciences
https://www.readbyqxmd.com/read/28079647/using-public-health-data-for-soil-pb-hazard-management-in-ohio
#4
John F Obrycki, Tyler Serafini, Darryl B Hood, Chris Alexander, Pam Blais, Nicholas T Basta
OBJECTIVES: To evaluate how frequently elevated soil lead (Pb) hazards (≥400 ppm Pb) were identified in existing blood Pb site investigations in Ohio. DESIGN: This study evaluated 3050 site investigations from the Ohio Department of Health for children with blood Pb levels at or above 10 μg dL that contained bare soil Pb data. SETTING: Data were collected from existing databases maintained by the Ohio Department of Health. PARTICIPANTS: All data were de-identified prior to analysis...
January 11, 2017: Journal of Public Health Management and Practice: JPHMP
https://www.readbyqxmd.com/read/28078415/gc-ms-method-validation-and-levels-of-methyl-eugenol-in-a-diverse-range-of-tea-tree-melaleuca-alternifolia-oils
#5
Carolyn A Raymond, Noel W Davies, Tony Larkman
Tea tree oil distilled from Melaleuca alternifolia has widespread use in the cosmetic industry as an antimicrobial as well as for other functions in topical products. Concerns were first raised by the European Commission's Scientific Committee on Consumer Products in 2004 about the level of the potentially carcinogenic phenylpropanoid compound methyl eugenol in tea tree oil. Limits on oil content in different types of cosmetic products were set based on a reported upper level of 0.9% methyl eugenol in the oil...
January 11, 2017: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/28077780/exposure-to-crvi-during-early-pregnancy-increases-oxidative-stress-and-disrupts-the-expression-of-antioxidant-proteins-in-placental-compartments
#6
Sakhila K Banu, Jone A Stanley, Kirthiram K Sivakumar, Robert J Taylor, Joe A Arosh, Robert C Burghardt
Epidemiologic studies document relationships between chromium VI (CrVI) exposure and increased risk of spontaneous abortion, stillbirth, preterm birth, and neonatal death in pregnant women. Environmental contamination with CrVI is a growing problem both in the United States and developing countries. CrVI is widely used in numerous industries. This study was designed to understand the mechanism of CrVI toxicity on placental oxidative stress and antioxidant (AOX) machinery. Pregnant mother rats were treated with or without CrVI (50 ppm K2Cr2O7) through drinking water from gestational day (GD) 9...
November 15, 2016: Toxicological Sciences: An Official Journal of the Society of Toxicology
https://www.readbyqxmd.com/read/28077733/identification-of-potential-plasma-biomarkers-for-nonalcoholic-fatty-liver-disease-by-integrating-transcriptomics-and-proteomics-in-laying-hens
#7
Meng-Tsz Tsai, Yu-Jen Chen, Ching-Yi Chen, Mong-Hsun Tsai, Chia-Li Han, Yu-Ju Chen, Harry J Mersmann, Shih-Torng Ding
BACKGROUND: Prevalent worldwide obesity is associated with increased incidence of nonalcoholic fatty liver disease (NAFLD) and metabolic syndrome. The identification of noninvasive biomarkers for NAFLD is of recent interest. Because primary de novo lipogenesis occurs in chicken liver as in human liver, adult chickens with age-associated steatosis resembling human NAFLD is an appealing animal model. OBJECTIVE: The objective of this study was to screen potential biomarkers in the chicken model for NAFLD by transcriptomic and proteomic analysis...
January 11, 2017: Journal of Nutrition
https://www.readbyqxmd.com/read/28077163/the-efficacy-of-varenicline-in-achieving-abstinence-among-waterpipe-tobacco-smokers-study-protocol-for-a-randomized-controlled-trial
#8
Raana Zahid, Omara Dogar, Sonia Mansoor, Amina Khan, Mona Kanaan, Mohammed Jawad, Jasjit S Ahluwalia, Kamran Siddiqi
BACKGROUND: Waterpipe tobacco smoking has increased among youth across the globe including in the US, and it continues as a common and traditional form of smoking tobacco in Pakistan. A range of behavioral and pharmacological therapies are available to support people in quitting cigarette smoking; however, little evidence exists for the efficacy of these therapies in achieving abstinence among waterpipe tobacco smokers. The objective of this study is to assess the efficacy of varenicline when added to behavioral support for waterpipe tobacco smoking cessation, by measuring biochemically validated continuous abstinence in waterpipe tobacco smokers...
January 11, 2017: Trials
https://www.readbyqxmd.com/read/28076495/antimicrobial-activity-of-ozone-and-naf-chlorhexidine-on-early-childhood-caries
#9
Marcos Ximenes, Mariane Cardoso, Fernando Astorga, Roland Arnold, Luiz André Pimenta, Ricardo de Sousa Viera
An early childhood carie (ECC) is an extremely destructive form of tooth decay. The aim of this study was to investigate the action of ozone (O3), and the association of sodium fluoride (NaF) with chlorhexidine (CHX) on bacteria related to ECC. Overnight culture of the bacteria was performed. On exponential phase the suspension was adjusted (101-108 CFU/mL). A drop (10μL) of each concentration of bacteria was applied on sheep blood agar plates and treated with O3 (2, 20, 200, and 2,000 ppm); after 18 hours, recovery analysis of CFU verified the reduction of bacterial activity...
January 5, 2017: Brazilian Oral Research
https://www.readbyqxmd.com/read/28073988/posteromedial-papillary-muscle-rupture-due-to-squeezing-of-the-left-anterior-descending-coronary-artery
#10
Kaoru Hattori, Kozo Fukui, Nobuaki Suzuki, Kazuo Ito
An unusual case of posteromedial papillary muscle (PPM) rupture due to isolated left anterior descending (LAD) artery ischaemia, associated with severe myocardial bridge contraction, is presented. The unusual blood supply to the PPM was associated with its apical origin and apex-forming LAD.
January 10, 2017: Interactive Cardiovascular and Thoracic Surgery
https://www.readbyqxmd.com/read/28073887/perioperative-predictors-of-permanent-pacing-and-long-term-dependence-following-tricuspid-valve-surgery-a-multicentre-analysis
#11
Philip L Mar, Christopher R Angus, Rajesh Kabra, Christopher K Migliore, Rohan Goswami, Leah A John, Yixi Tu, Rakesh Gopinathannair
AIMS: Permanent pacemaker placement (PPM) is often required after valvular surgery and is especially common following tricuspid valve surgery [tricuspid valve repair or replacement (TVR)]. Literature suggests that surgical intervention for isolated tricuspid valve disease is becoming more prevalent. Predictors of PPM dependency following TVR are currently unknown and would be clinically useful from a prognostication standpoint. METHODS AND RESULTS: We conducted a multicentre, retrospective study to assess perioperative factors of TVR that predispose to PPM placement and long-term PPM dependency from 2008 to 2014...
January 10, 2017: Europace: European Pacing, Arrhythmias, and Cardiac Electrophysiology
https://www.readbyqxmd.com/read/28072890/candida-albicans-survival-growth-and-biofilm-formation-are-differently-affected-by-mouthwashes-an-in-vitro-study
#12
Simona Paulone, Giulia Malavasi, Andrea Ardizzoni, Carlotta Francesca Orsi, Samuele Peppoloni, Rachele Giovanna Neglia, Elisabetta Blasi
Candida albicans is the most common cause of oral mycoses. The aim of the present study was to investigate in vitro the susceptibility of C. albicans to mouthwashes, in terms of growth, survival and biofilm formation. Candida albicans, laboratory strain SC5314, and 7 commercial mouthwashes were employed: 3 with 0.2% chlorhexidine digluconate; 1 with 0.06% chlorhexidine digluconate and 250 ppm F- sodium fluoride; 3 with fluorine-containing molecules. None of the mouthwashes contained ethanol in their formulations...
January 9, 2017: New Microbiologica
https://www.readbyqxmd.com/read/28070315/evaluation-of-analytical-methods-for-the-determination-of-the-physicochemical-properties-of-fermented-granulated-and-roasted-cassava-pulp-gari
#13
Cornelia Felber, Yaovi Ouézou Azouma, Marcus Reppich
Simple but reliable methods for the determination of the physicochemical properties of gari were evaluated for the parameters, such as grain size, bulk density, swelling index, moisture content, gross calorific value, cyanide content, and acidity content. The grain sizes were between 525 and 928 μm (weighted means), the bulk densities between 0.541 and 0.699 g/cm³, and the swelling indices between 3.21 and 4.33. The moisture contents ranged from 4.30 to 9.19%. The gross calorific values were found between 15...
January 2017: Food Science & Nutrition
https://www.readbyqxmd.com/read/28070311/optimization-of-production-and-quality-evaluation-of-maize-based-snack-supplemented-with-soybean-and-tiger-nut-cyperus-esculenta-flour
#14
Olugbenga O Awolu, Olufunmilayo S Omoba, Olumide Olawoye, Modupe Dairo
Optimization of the production and evaluation of the quality of maize-based snack supplemented with soy and tigernut flour was carried out. Experimental design for the composite flour was carried out using optimal design model of response surface methodology. The variables were toasted maize flour (75-85%), soy flour (10-20%), and tigernut flour (5-10%); while the responses were proximate composition and mineral contents. Three blends were selected from optimization results; runs 2, 7, and 11. The functional properties, pasting properties, antioxidant, antinutritional, and amino acid profile of the three blends were evaluated...
January 2017: Food Science & Nutrition
https://www.readbyqxmd.com/read/28070102/altered-susceptibility-of-an-obese-rat-model-to-13-week-subchronic-toxicity-induced-by-3-monochloropropane-1-2-diol
#15
Takeshi Toyoda, Young-Man Cho, Jun-Ichi Akagi, Yasuko Mizuta, Kohei Matsushita, Akiyoshi Nishikawa, Katsumi Imaida, Kumiko Ogawa
3-Monochloropropane-1,2-diol (3-MCPD) is a heat-induced food contaminant that has been shown to be a nongenotoxic renal carcinogen. Although the toxicity of 3-MCPD has been widely investigated for decades, there is a further concern that 3-MCPD might exert more potent toxicity in high-risk population with underlying diseases such as hyperlipidemia associated with obesity. In the present study, we performed a 13-week subchronic toxicity study for 3-MCPD using an obesity rat model to investigate the differences in susceptibility between obese and normal individuals...
2017: Journal of Toxicological Sciences
https://www.readbyqxmd.com/read/28070083/facile-and-sensitive-detection-of-carbofuran-carbamate-pesticide-in-rice-and-soybean-using-coupling-reaction-based-surface-enhanced-raman-scattering
#16
Thanyada Sukmanee, Kanet Wongravee, Sanong Ekgasit, Chuchaat Thammacharoen, Prompong Pienpinijtham
In this research, a sensitive and selective method for detecting one of the most toxic insecticides, "carbofuran", in rice and soybean is presented. This method is based on the coupling reaction of diazonium ion combined with a surface-enhanced Raman scattering technique. Diazonium ion produced from p-aminothiophenol reacts specifically with carbofuran phenol from the hydrolysis of carbofuran. The generated azo compounds attach to the surface of silver nanoparticles via the Ag-S bond. Therefore, a strong Raman intensity can be obtained...
2017: Analytical Sciences: the International Journal of the Japan Society for Analytical Chemistry
https://www.readbyqxmd.com/read/28069835/multielectrode-vs-point-by-point-mapping-for-ventricular-tachycardia-substrate-ablation-a-randomized-study
#17
Juan Acosta, Diego Penela, David Andreu, Mario Cabrera, Alicia Carlosena, Francesca Vassanelli, Francisco Alarcón, David Soto-Iglesias, Viatcheslav Korshunov, Roger Borras, Markus Linhart, Mikel Martínez, Juan Fernández-Armenta, Lluis Mont, Antonio Berruezo
AIMS: Ventricular tachycardia (VT) substrate ablation is based on detailed electroanatomical maps (EAM). This study analyses whether high-density multielectrode mapping (MEM) is superior to conventional point-by-point mapping (PPM) in guiding VT substrate ablation procedures. METHODS AND RESULTS: This was a randomized controlled study (NCT02083016). Twenty consecutive ischemic patients undergoing VT substrate ablation were randomized to either group A [n = 10; substrate mapping performed first by PPM (Navistar) and secondly by MEM (PentaRay) ablation guided by PPM] or group B [n = 10; substrate mapping performed first by MEM and second by PPM ablation guided by MEM]...
January 8, 2017: Europace: European Pacing, Arrhythmias, and Cardiac Electrophysiology
https://www.readbyqxmd.com/read/28068894/strain-differences-in-a-murine-model-of-air-pollutant-induced-nonatopic-asthma-and-rhinitis
#18
Jack R Harkema, Lucas A Hotchkiss, Nicholas A Vetter, Daven N Jackson-Humbles, Ryan P Lewandowski, James G Wagner
Ozone is an irritating gas found in photochemical smog. Epidemiological associations have been made between the onset of asthma and childhood exposures to increasing levels of ambient ozone (i.e., air pollutant-induced nonatopic asthma). Individuals, however, vary in their susceptibility to this outdoor air pollutant, which may be due, in part, to their genetic makeup. The present study was designed to test the hypothesis that there are murine strain-dependent differences in pulmonary and nasal pathologic responses to repeated ozone exposures...
January 2017: Toxicologic Pathology
https://www.readbyqxmd.com/read/28068841/listeria-monocytogenes-behaviour-and-quality-attributes-during-sausage-storage-affected-by-sodium-nitrite-sodium-lactate-and-thyme-essential-oil
#19
Carla María Blanco-Lizarazo, Rubén Betancourt-Cortés, Angélica Lombana, Katerine Carrillo-Castro, Indira Sotelo-Díaz
The effects of the addition of nitrite at 200 ppm (N), sodium lactate 1.5% (L) and thyme essential oil at 100 ppm (T1) on Listeria monocytogenes behaviour and ATPase activity inhibition were evaluated, as well as lipid oxidation through the quantification of malonaldehydes, in sausage stored at 8 ℃ for 41 days and at 30 ℃ for 14 days. The changes in the colour profile were performed during storage time at 8 ℃. Quantitative descriptive sensory analyses were performed after two days at 4 ℃...
January 1, 2017: Food Science and Technology International, Ciencia y Tecnología de Los Alimentos Internacional
https://www.readbyqxmd.com/read/28068433/molecular-and-functional-characterization-of-gr2-r1-event-based-backcross-derived-lines-of-golden-rice-in-the-genetic-background-of-a-mega-rice-variety-swarna
#20
Haritha Bollinedi, Gopala Krishnan S, Kumble Vinod Prabhu, Nagendra Kumar Singh, Sushma Mishra, Jitendra P Khurana, Ashok Kumar Singh
Homozygous Golden Rice lines developed in the background of Swarna through marker assisted backcross breeding (MABB) using transgenic GR2-R1 event as a donor for the provitamin A trait have high levels of provitamin A (up to 20 ppm) but are dwarf with pale green leaves and drastically reduced panicle size, grain number and yield as compared to the recurrent parent, Swarna. In this study, we carried out detailed morphological, biochemical and molecular characterization of these lines in a quest to identify the probable reasons for their abnormal phenotype...
2017: PloS One
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