keyword
MENU ▼
Read by QxMD icon Read
search

forskolin

keyword
https://www.readbyqxmd.com/read/28920413/functional-role-of-forskolin-and-pd166285-in-the-development-of-denuded-mouse-oocytes
#1
Hongguo Cao, Yani Bian, Fei Zhang, Yunshu Tang, Caixia Li, Jiemei Chen, Xiaorong Zhang
Objective: cAMP and MPF play critical roles during the maturation of mammalian oocytes. The aim of this study was to produce the offspring from denuded oocytes in mice by regulating cAMP and MPF. Methods: In this study, we used DOs at the germinal vesicle (GV) stage in mice and regulated levels of cAMP and MPF in DOs by adding Forskolin and PD166285 during in vitro maturation (IVM) without FSH and LH respectively. Results: Combined use of 50 μM Forskolin for 3 h and 2...
September 18, 2017: Asian-Australasian Journal of Animal Sciences
https://www.readbyqxmd.com/read/28916831/the-melanocortin-signaling-camp-axis-accelerates-repair-and-reduces-mutagenesis-of-platinum-induced-dna-damage
#2
Stuart G Jarrett, Katharine M Carter, Brent J Shelton, John A D'Orazio
Using primary melanocytes and HEK293 cells, we found that cAMP signaling accelerates repair of bi- and mono-functional platinum-induced DNA damage. Elevating cAMP signaling either by the agonistic MC1R ligand melanocyte stimulating hormone (MSH) or by pharmacologic cAMP induction by forskolin enhanced clearance of intrastrand cisplatin-adducts in melanocytes or MC1R-transfected HEK293 cells. MC1R antagonists human beta-defensin 3 and agouti signaling protein blocked MSH- but not forskolin-mediated enhancement of platinum-induced DNA damage...
September 15, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28912066/the-camp-effectors-pka-and-epac-activate-endothelial-no-synthase-through-pi3k-akt-pathway-in-human-endothelial-cells
#3
Verónica García-Morales, María Luaces-Regueira, Manuel Campos-Toimil
3',5'-cyclic adenosine monophosphate (cAMP) exerts an endothelium-dependent vasorelaxant action by stimulating endothelial NO synthase (eNOS) activity, and the subsequent NO release, through cAMP protein kinase (PKA) and exchange protein directly activated by cAMP (Epac) activation in endothelial cells. We have here investigated the mechanism by which the cAMP-Epac/PKA pathway activates eNOS. cAMP-elevating agents (forskolin and dibutyryl-cAMP) and the joint activation of PKA (6-Bnz-cAMP) and Epac (8-pCPT-2'-O-Me-cAMP) increased cytoplasmic Ca(2+) concentration ([Ca(2+)]c) in ≤ 30% of fura-2-loaded isolated human umbilical vein endothelial cells (HUVEC)...
September 11, 2017: Biochemical Pharmacology
https://www.readbyqxmd.com/read/28901508/a-novel-method-for-obtaining-highly-enriched-schwann-cell-populations-from-mature-monkey-nerves-based-on-in-vitro-pre%C3%A2-degeneration
#4
Gangyang Wang, Zhengwen Ma, Lingling Cao, Guofeng Yan, Yang Wang, Yuqing Jin, Hua Shen, Yiping Zhang, Xiaoming Xu, Xuejin Chen, Zunli Shen
Schwann cells (SCs) are indispensable for cell therapy and tissue engineering of the peripheral nervous system. Easy access to activated, highly proliferative SCs is necessary for clinical applications. The present study developed a fast, efficient method for obtaining highly purified SCs from the peripheral nerve of a mature Rhesus monkey. The common peroneal nerves of 4‑year‑old Rhesus monkeys were harvested and subjected to in vitro pre‑degeneration in a modified SC culture medium (SCCM). The nerve pieces were subsequently treated enzymatically to dissociate the cells and then cultured for 2 days in SCCM...
September 5, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28882822/both-nherf3-and-nherf2-are-necessary-for-multiple-aspects-of-acute-regulation-of-nhe3-by-elevated-ca-2-cgmp-and-lysophosphatidic-acid
#5
Leela Rani Avula, Tiane Chen, Olga Kovbasnjuk, Mark Donowitz
The intestinal epithelial brush border Na(+)/H(+) exchanger NHE3 accounts for a large component of intestinal Na absorption. NHE3 is regulated during digestion by signaling complexes on its C-terminus that include the four multi-PDZ domain containing NHERF family proteins. Because the roles of each NHERF appear to vary based on the cell model or intestinal segment studied and because of our recent finding that a NHERF3-NHERF2 heterodimer appears important for NHE3 regulation in Caco-2 cells, we examined the role of NHERF3 and NHERF2 in homozygous C57BL/6 NHERF2 and NHERF3 KO mouse jejunum...
September 7, 2017: American Journal of Physiology. Gastrointestinal and Liver Physiology
https://www.readbyqxmd.com/read/28878120/mdm2-regulates-cardiac-contractility-by-inhibiting-grk2-mediated-desensitization-of-%C3%AE-adrenergic-receptor-signaling
#6
Pierre-Yves Jean-Charles, Samuel Mon-Wei Yu, Dennis Abraham, Reddy Peera Kommaddi, Lan Mao, Ryan T Strachan, Zhu-Shan Zhang, Dawn E Bowles, Leigh Brian, Jonathan A Stiber, Stephen N Jones, Walter J Koch, Howard A Rockman, Sudha K Shenoy
The oncoprotein Mdm2 is a RING domain-containing E3 ubiquitin ligase that ubiquitinates G protein-coupled receptor kinase 2 (GRK2) and β-arrestin2, thereby regulating β-adrenergic receptor (βAR) signaling and endocytosis. Previous studies showed that cardiac Mdm2 expression is critical for controlling p53-dependent apoptosis during early embryonic development, but the role of Mdm2 in the developed adult heart is unknown. We aimed to identify if Mdm2 affects βAR signaling and cardiac function in adult mice...
September 7, 2017: JCI Insight
https://www.readbyqxmd.com/read/28861508/affinity-and-efficacy-studies-of-tetrahydrocannabinolic-acid-a-at-cannabinoid-receptor-types-one-and-two
#7
John M McPartland, Christa MacDonald, Michelle Young, Phillip S Grant, Daniel P Furkert, Michelle Glass
Introduction:Cannabis biosynthesizes Δ(9)-tetrahydrocannabinolic acid (THCA-A), which decarboxylates into Δ(9)-tetrahydrocannabinol (THC). There is growing interest in the therapeutic use of THCA-A, but its clinical application may be hampered by instability. THCA-A lacks cannabimimetic effects; we hypothesize that it has little binding affinity at cannabinoid receptor 1 (CB1). Materials and Methods: Purity of certified reference standards were tested with high performance liquid chromatography (HPLC). Binding affinity of THCA-A and THC at human (h) CB1 and hCB2 was measured in competition binding assays, using transfected HEK cells and [(3)H]CP55,940...
2017: Cannabis and Cannabinoid Research
https://www.readbyqxmd.com/read/28859856/gastrin-induces-nuclear-export-and-proteasome-degradation-of-menin-in-enteric-glial-cells
#8
Sinju Sundaresan, Cameron A Meininger, Anthony J Kang, Amanda L Photenhauer, Michael M Hayes, Nirakar Sahoo, Jolanta Grembecka, Tomasz Cierpicki, Lin Ding, Thomas J Giordano, Tobias Else, David J Madrigal, Malcolm J Low, Fiona Campbell, Ann-Marie Baker, Haoxing Xu, Nicholas A Wright, Juanita L Merchant
BACKGROUND & AIMS: The multiple endocrine neoplasia, type 1 (MEN1) locus encodes the nuclear protein and tumor suppressor menin. MEN1 mutations frequently cause neuroendocrine tumors (NETs) such as gastrinomas, characterized by their predominant duodenal location and local metastasis at time of diagnosis. Diffuse gastrin cell hyperplasia precedes the appearance of MEN1 gastrinomas, which develop within submucosal Brunner's glands. We investigated how menin regulates expression of the gastrin gene and induces generation of submucosal gastrin-expressing cell hyperplasia...
August 28, 2017: Gastroenterology
https://www.readbyqxmd.com/read/28857544/identification-of-5-1-methyl-5-trifluoromethyl-1h-pyrazol-3-yl-thiophene-2-carboxamides-as-novel-and-selective-monoamine-oxidase-b-inhibitors-used-to-improve-memory-and-cognition
#9
Alan P Kaplan, Terence Keenan, Roderick Scott, Xianbo Zhou, Rusiko Bourchouladze, Andrew J McRiner, Mark E Wilson, Darlene Romashko, Regina Miller, Matthew Bletsch, Gary Anderson, Jennifer Stanley, Adia Zhang, Dong Lee, John Nikpur
Initial work in Drosophila and mice demonstrated that the transcription factor cyclic adenosine monophosphate (cAMP) response element binding protein (CREB) is a master control gene for memory formation. The relationship between CREB and memory has also been found to be true in other species, including aplysia and rats. It is thus well-established that CREB activation plays a central role in memory enhancement and that CREB is activated during memory formation. On the basis of these findings, a phenotypic high-throughput screening campaign utilizing a CRE-luciferase (CRE-Luci) SK-N-MC cell line was performed to identify compounds that enhance transcriptional activation of the CRE promoter with a suboptimal dose of forskolin...
September 20, 2017: ACS Chemical Neuroscience
https://www.readbyqxmd.com/read/28855436/effects-of-sorafenib-and-an-adenylyl-cyclase-activator-on-in-vitro-growth-of-well-differentiated-thyroid-cancer-cells
#10
Aya Sawa, Tomohiro Chiba, Jun Ishii, Hiroyuki Yamamoto, Hisato Hara, Hiroshi Kamma
Well-differentiated thyroid carcinomas have driver mutations involving growth factor receptor-tyrosine kinases (RTKs) or their intracellular signaling pathway, that is, the mitogen-activated protein kinase (MAPK) pathway. Sorafenib is a multikinase inhibitor of RTKs and the MAPK pathway and has recently been used for the treatment of unresectable well-differentiated thyroid carcinoma. In normal thyroid follicular cells, stimulation of the thyroid-stimulating hormone (TSH) receptor activates the cyclic adenosine monophosphate (cAMP) pathway and promotes cell growth as well as hormonal secretion...
August 31, 2017: Endocrine Journal
https://www.readbyqxmd.com/read/28851815/epac-rap1-axis-mediated-switch-in-the-response-of-primary-and-metastatic-melanoma-to-cyclic-amp
#11
Carlos I Rodriguez, Edgardo Castro-Perez, Kirthana Prabhakar, Laura Block, B Jack Longley, Jaclyn A Wisinski, Michelle E Kimple, Vijayasaradhi Setaluri
Cyclic AMP (cAMP) is an important second messenger that regulates a wide range of physiological processes. In mammalian cutaneous melanocytes, cAMP-mediated signaling pathways activated by G-protein coupled receptors (GPCRs), like melanocortin 1 receptor (MC1R), play critical roles in melanocyte homeostasis including cell survival, proliferation and pigment synthesis. Impaired cAMP signaling is associated with increased risk of cutaneous melanoma. Whereas mutations in mitogen activated protein kinase (MAPK) pathway components are the most frequent oncogenic drivers of melanoma, the role of cAMP in melanoma is not well understood...
August 29, 2017: Molecular Cancer Research: MCR
https://www.readbyqxmd.com/read/28839011/eye-and-heart-morphogenesis-are-dependent-on-the-melatonin-signaling-in-chick-embryos
#12
Renato C Nogueira, Lucia de Fatima S Sampaio
Calmodulin is vital for chick embryos morphogenesis in the incubation time 48 h to 66 h when the rudimentary C-shaped heart attains an S-shaped pattern and the optic vesicles develops to the optic cups. Melatonin is in the extraembryonic yolk sac of the avian egg. Melatonin binds calmodulin. The aim of this study was to investigate the melatonin functions in the formation of the chick embryo optic cups and S-shaped heart, by pharmacology and immunoassays methods. Mel1a melatonin receptor immunofluorescence was distributed in the optic cups and rudimentary hearts...
August 24, 2017: Journal of Experimental Biology
https://www.readbyqxmd.com/read/28838808/characterization-of-structurally-novel-g-protein-biased-cb1-agonists-implications-for-drug-development
#13
Benjamin M Ford, Lirit N Franks, Sherrica Tai, William E Fantegrossi, Edward L Stahl, Michael D Berquist, Christian V Cabanlong, Catheryn D Wilson, Narsimha R Penthala, Peter A Crooks, Paul L Prather
The human cannabinoid subtype 1 receptor (hCB1R) is highly expressed in the CNS and serves as a therapeutic target for endogenous ligands as well as plant-derived and synthetic cannabinoids. Unfortunately, acute use of hCB1R agonists produces unwanted psychotropic effects and chronic administration results in development of tolerance and dependence, limiting the potential clinical use of these ligands. Studies in β-arrestin knockout mice suggest that interaction of certain GPCRs, including μ-, δ-, κ-opioid and hCB1Rs, with β-arrestins might be responsible for several adverse effects produced by agonists acting at these receptors...
August 21, 2017: Pharmacological Research: the Official Journal of the Italian Pharmacological Society
https://www.readbyqxmd.com/read/28838692/synthesis-of-novel-forskolin-isoxazole-derivatives-with-potent-anti-cancer-activity-against-breast-cancer-cell-lines
#14
Srinivas Burra, Vani Voora, Ch Prasad Rao, P Vijay Kumar, Rama Krishna Kancha, G L David Krupadanam
Forskolin C1-isoxazole derivatives (3,5-regioisomers) (11a-e, 14, 15a-h and 15, 16a-g) were synthesized regioselectively by adopting 1,3-dipolar cycloadditions. These derivatives were tested using estrogen receptor positive breast cancer cell lines MCF-7 and BT-474. Majority of the compounds exhibited activity against the p53-positive MCF-7 breast cancer cells but not against the p53-negative BT-474 breast cancer cells. Among forskolin derivatives, compounds 11a, 11c, 14a, 14f, 14g, 14h, 15b, 16g and 17b exhibited higher anti-cancer activity against MCF-7 cell line with an IC50≤1µM...
September 15, 2017: Bioorganic & Medicinal Chemistry Letters
https://www.readbyqxmd.com/read/28835591/rasd1-is-an-estrogen-responsive-immediate-early-gene-and-modulates-expression-of-late-genes-in-rat-anterior-pituitary-cells
#15
Linghong Wang, Tetsuo Mitsui, Maho Ishida, Michi Izawa, Jun Arita
Dexamethasone-induced Ras-related protein 1 (Rasd1) is a member of the Ras superfamily of monomeric G proteins that have a regulatory function in signal transduction. Here we investigated the role of Rasd1 in regulating estrogen-induced gene expression in primary cultures of rat anterior pituitary cells. Rasd1 mRNA expression in anterior pituitary cells decreased after treatment with forskolin or serum and increased after treatment with 17β-estradiol (E2). Increases in Rasd1 mRNA expression occurred as early as 0...
August 23, 2017: Endocrine Journal
https://www.readbyqxmd.com/read/28826686/cofilin-is-a-camp-effector-in-mediating-actin-cytoskeleton-reorganization-and-steroidogenesis-in-mouse-and-human-adrenocortical-tumor-cells
#16
E Peverelli, R Catalano, E Giardino, D Treppiedi, V Morelli, C L Ronchi, A Vaczlavik, N Fusco, S Ferrero, J Bertherat, F Beuschlein, I Chiodini, M Arosio, A Spada, G Mantovani
cAMP pathway plays a major role in the pathogenesis of cortisol-producing adrenocortical adenomas (CPA). cAMP-induced steroidogenesis is preceded by actin cytoskeleton reorganization, a process regulated by cofilin activity. In this study we investigated cofilin role in mediating cAMP effects on cell morphology and steroidogenesis in adrenocortical tumor cells. We demonstrated that forskolin induced cell rounding and strongly reduced phosphorylated (P)-cofilin/total cofilin ratio in Y1 (-52 ± 16%, p < 0...
October 10, 2017: Cancer Letters
https://www.readbyqxmd.com/read/28813646/antihelminthic-drug-niclosamide-inhibits-cip2a-and-reactivates-tumor-suppressor-protein-phosphatase-2a-in-non-small-cell-lung-cancer-cells
#17
Myeong-Ok Kim, Min Ho Choe, Yi Na Yoon, Jiyeon Ahn, Minjin Yoo, Kwan-Young Jung, Sungkwan An, Sang-Gu Hwang, Jeong Su Oh, Jae-Sung Kim
Protein phosphatase 2A (PP2A) is a critical tumor suppressor complex responsible for the inactivation of various oncogenes. Recently, PP2A reactivation has emerged as an anticancer strategy. Cancerous inhibitor of protein phosphatase 2A (CIP2A), an endogenous inhibitor of PP2A, is upregulated in many cancer cells, including non-small cell lung cancer (NSCLC) cells. We demonstrated that the antihelminthic drug niclosamide inhibited the expression of CIP2A and reactivated the tumor suppressor PP2A in NSCLC cells...
August 13, 2017: Biochemical Pharmacology
https://www.readbyqxmd.com/read/28808448/relevance-of-the-nr4a-sub-family-of-nuclear-orphan-receptors-in-trophoblastic-bewo-cell-differentiation
#18
Sudha Saryu Malhotra, Satish Kumar Gupta
Nur-77, a member of the NR4A sub-family of nuclear orphan receptors, is downregulated in the placentae of pre-eclamptic women. Here, we investigate the relevance of Nor-1, Nurr-1 and Nur-77 in trophoblastic cell differentiation. Their transcript levels were found to be significantly upregulated in BeWo cells treated with forskolin. The maximum increase was observed after 2 h, with a second peak in the expression levels after 48 h. The expression of NR4A sub-family members was also found to be upregulated in BeWo cells after treatment with hCG and GnRH...
2017: Cellular & Molecular Biology Letters
https://www.readbyqxmd.com/read/28800037/primary-cilia-deficiency-induces-intracranial-aneurysm
#19
Min Liu, Jizong Zhao, Qian Zhou, Yong Peng, Yu Zhou, Yugang Jiang
BACKGROUND: Intracranial aneurysm (IA) rupture is life-threatening. However, the mechanisms underlying IA initiation, progression, and rupture remain poorly understood. In the present study, we examined the role of primary cilia in IA development. RESULTS: IA was experimentally induced in mice with elastase and angiotensin II treatment. The number of cells with primary cilia was determined in both IA and peri-IA regions. The role of primary cilia in IA development was assessed through knocking out or manipulating the expression of important components of primary cilia...
August 9, 2017: Shock
https://www.readbyqxmd.com/read/28798775/maintenance-of-a-schwann-like-phenotype-in-differentiated-adipose-derived-stem-cells-requires-the-synergistic-action-of-multiple-growth-factors
#20
Alice E Mortimer, Alessandro Faroni, Mahmut A Kilic, Adam J Reid
Differentiating human adipose-derived stem cells (ASCs) towards Schwann cells produces an unstable phenotype when stimulating factors are withdrawn. Here, we set out to examine the role of glial growth factor 2 (GGF-2) in the maintenance of Schwann-like cells. Following ASC differentiation to Schwann-like cells, stimulating factors were withdrawn such that cells either remained in media supplemented with all stimulating factors, GGF-2 alone, or underwent complete withdrawal of all factors. Furthermore, each stimulating factor was also removed from the growth medium individually...
2017: Stem Cells International
keyword
keyword
37253
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"