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https://www.readbyqxmd.com/read/29150274/neuroprotective-potential-of-high-dose-biotin
#1
Mark F McCarty, James J DiNicolantonio
A recent controlled trial has established that high-dose biotin supplementation - 100 mg, three times daily - has a stabilizing effect on progression of multiple sclerosis (MS). Although this effect has been attributed to an optimization of biotin's essential cofactor role in the brain, a case can be made that direct stimulation of soluble guanylate cyclase (sGC) by pharmacological concentrations of biotin plays a key role in this regard. The utility of high-dose biotin in MS might reflect an anti-inflammatory effect of cGMP on the cerebral microvasculature, as well on oligodendrocyte differentiation and on Schwann cell production of neurotrophic factors thought to have potential for managing MS...
November 2017: Medical Hypotheses
https://www.readbyqxmd.com/read/29148722/acoustic-wave-driven-functionalized-particles-for-aptamer-based-target-biomolecule-separation
#2
Raheel Ahmad, Ghulam Destgeer, Muhammad Afzal, Jinsoo Park, Husnain Ahmed, Jin Ho Jung, Kwangseok Park, Tae-Sung Yoon, Hyung Jin Sung
We developed a hybrid microfluidic device that utilized acoustic waves to drive functionalized microparticles inside a continuous flow microchannel and to separate particle-conjugated target proteins from a complex fluid. The acoustofluidic device comprised of an interdigitated transducer (IDT) that produced high-frequency surface acoustic waves (SAW) in a polydimethylsiloxane (PDMS) microfluidic channel. The SAW interacted with the sample fluid flow inside the microchannel and deflected particles from their original streamlines to achieve separation...
November 17, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/29148720/a-dual-signal-readout-nanosphere-for-rapid-point-of-care-detection-of-ebola-virus-glycoprotein
#3
Jiao Hu, Yong-Zhong Jiang, Ling-Ling Wu, Zhen Wu, Yuhai Bi, Gary Wong, Xiang-Guo Qiu, Jian-Jun Chen, Dai-Wen Pang, Zhi-Ling Zhang
Rapid detection of highly contagious pathogens is the key to increasing the probability of survival and reducing infection rates. We developed a sensitive and quantitative lateral flow assay for detection of EBOV glycoprotein with a novel multifunctional nanosphere (RNs@Au) as a reporter. Each RNs@Au contains hundreds of quantum dots and dozens of Au nanoparticles and can achieve enhanced dual-signal readout (fluorescence signal for quantitative detection and colorimetric signal for visual detection). Antibody (Ab) and streptavidin (SA) were simultaneously modified onto the RNs@Au to label the target as well as to act as the signal enhancer...
November 17, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/29147710/step-by-step-build-up-of-covalent-poly-ethylene-oxide-nanogel-films
#4
S Zahouani, L Hurman, M De Giorgi, C Vigier-Carrière, F Boulmedais, B Senger, B Frisch, P Schaaf, P Lavalle, L Jierry
Hydrogels based on poly(ethylene glycol) (PEG) are commonly used for studies related to cell fate and tissue engineering. Here we present a new covalent layer-by-layer build-up process leading to PEG coatings of nanometer size called "nanogel films". Compared to macroscopic hydrogels, such nanogels should provide a fine control over the structure and the thickness of the coating. Alternated deposition of bifunctional and tetra functional PEG molecules reacting through thiol/maleimide click chemistry is evaluated by quartz crystal microbalance...
November 17, 2017: Nanoscale
https://www.readbyqxmd.com/read/29146181/proximity-labeling-of-cis-ligands-of-cd22-siglec-2-reveals-stepwise-%C3%AE-2-6-sialic-acid-dependent-and-independent-interactions
#5
Amin Alborzian Deh Sheikh, Chizuru Akatsu, Akihiro Imamura, Hajjaj H M Abdu-Allah, Hiromu Takematsu, Hiromune Ando, Hideharu Ishida, Takeshi Tsubata
Lectins expressed on the cell surface are often bound and regulated by the membrane molecules containing the glycan ligands on the same cell (cis-ligands). However, molecular nature and function of cis-ligands are generally poorly understood partly because of weak interaction between lectins and glycan ligands. Cis-ligands are most extensively studied in CD22 (also known as Siglec-2), an inhibitory B lymphocyte receptor specifically recognizing α2,6 sialic acids. CD22, CD45 and IgM are suggested to be ligands of CD22...
November 13, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29142190/detection-of-atherosclerotic-plaques-in-the-rabbit-aorta-using-ultrasound-microbubbles-conjugated-to-interleukin-18-antibodies
#6
Zhen-Zhen Jiang, Xia-Tian Liu, Cai-Ye Ma, Cong He, Xing-Yun Li, Chuan-Lin Hou, Zu-Sheng Cheng, Guo-Yuan Xia
BACKGROUND The purpose of the study was to investigate the ability of microbubbles (MBs) targeting interleukin-18 (IL-18) to detect plaques in a rabbit atherosclerotic plaque model. MATERIAL AND METHODS A rabbit atherosclerotic plaque model was established. The locations of the atherosclerotic plaques were verified by two-dimensional scanning and color Doppler flow imaging. An IL-18 antibody was conjugated to naked MBs (MBc) using the biotin-streptavidin conjugation method, resulting in the formation of MBIL-18...
November 16, 2017: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
https://www.readbyqxmd.com/read/29141796/high-dose-biotin-as-treatment-for-progressive-multiple-sclerosis
#7
Gary Birnbaum, Jessica Stulc
BACKGROUND: Published data suggested high dose biotin improved patients with progressive MS. We wished to determine benefits and side effects of administering daily high dose biotin to patients with progressive multiple sclerosis in a large MS specialty clinic. METHODS: Forty-three patients with progressive multiple scleroses were prescribed pharmaceutical grade biotin as a single daily dose of 300mg/day. Brain MRIs were performed at baseline and after one year on biotin...
November 2017: Multiple Sclerosis and related Disorders
https://www.readbyqxmd.com/read/29141120/biotinylated-photopolymers-for-3d-printed-unibody-lab-on-a-chip-optical-platforms
#8
Caterina Credi, Gianmarco Griffini, Marinella Levi, Stefano Turri
The present work reports the first demonstration of straightforward fabrication of monolithic unibody lab-on-a-chip (ULOCs) integrating bioactive micrometric 3D scaffolds by means of multimaterial stereolithography (SL). To this end, a novel biotin-conjugated photopolymer is successfully synthesized and optimally formulated to achieve high-performance SL-printing resolution, as demonstrated by the SL-fabrication of biotinylated structures smaller than 100 µm. By optimizing a multimaterial single-run SL-based 3D-printing process, such biotinylated microstructures are incorporated within perfusion microchambers whose excellent optical transparency enables real-time optical microscopy analyses...
November 15, 2017: Small
https://www.readbyqxmd.com/read/29140695/rna-g-quadruplexes-in-kirsten-ras-kras-oncogene-as-targets-for-small-molecules-inhibiting-translation
#9
Giulia Miglietta, Susanna Cogoi, Jessica Marinello, Giovanni Capranico, Alexander S Tikhomirov, Andrey E Shchekotikhin, Luigi E Xodo
The human KRAS transcript contains a G-rich 5'-UTR sequence (77 % GC) harbouring several G4 motifs capable to form stable RNA G-quadruplex (RG4) structures that can serve as targets for small molecules. A biotin-streptavidin pull-down assay showed that 4,11-bis(2-aminoethylamino)anthra[2,3-b]furan-5,10-dione (2a) binds to RG4s in the KRAS transcript under low-abundance cellular conditions. Dual-luciferase assays demonstrated that 2a and its analogue 4,11-bis(2-aminoethylamino)anthra[2,3-b]thiophene-5,10-dione 2b) repress translation in a dose-dependent manner...
November 15, 2017: Journal of Medicinal Chemistry
https://www.readbyqxmd.com/read/29140078/molecular-recognition-and-immobilization-of-ligand-conjugated-redox-responsive-polymer-nanocontainers
#10
Wilke De Vries, Matthias Tesch, Armido Studer, Bart Jan Ravoo
We present the preparation of ligand conjugated redox-responsive polymer nanocontainers by the supramolecular decoration of cyclodextrin vesicles with a thin redox-cleavable polymer shell that displays molecular recognition units on its surface. Two widely different recognition motifs (mannose-Concanavalin A and biotin-streptavidin) are compared and the impact of ligand density is studied using aggregation assays, dynamic light scattering, and a fluorometric quantification of ligands. Microcontact printing is used to prepare streptavidin-patterned surfaces and the specific immobilization of biotin conjugated nanocontainers is demonstrated...
November 15, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/29135238/elucidating-protein-ligand-recognition-with-combined-surface-plasmon-resonance-and-surface-enhanced-raman-spectroscopy
#11
Ju-Young Kim, Zhi-Cong Zeng, Lifu Xiao, Zachary D Schultz
The ability to distinguish between specific and non-specific binding is important for assessing the interactions between protein receptors and ligands. Surface plasmon resonance (SPR) spectroscopy is an advanced tool to measure the binding events, yet the ability to distinguish between specific and non-specific binding remains a limitation. To address this problem, we use SPR spectroscopy correlated with surface enhanced Raman scattering (SERS). The chemical information present in SERS spectra provides insight into the molecular interactions between functionalized nanoparticles and proteins, which are not detectable by SPR alone...
November 14, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/29135114/expression-and-role-of-p53-in-oral-lichen-planus-patients
#12
Milos Hadzi-Mihailovic, Renata Petrovic, Helene Raybaud, Dragan Stanimirovic, Meltem Ozar Koray
PURPOSE: Oral lichen planus (OLP) is an autoimmune skin and mucosal disorder. The range of malignant transformation in OLP varies between 0.1-3%. p53 is a tumor suppressor protein. Defective p53 could allow abnormal cells to proliferate, resulting in cancer. p53 plays an important role in cell cycle control and apoptosis and loss of p53 function has been demonstrated in about half of all human cancers. The purpose of the study was to investigate the malignant potential of OLP on the basis of p53 expression and to correlate p53 expression with clinical and histopathological features of OLP...
September 2017: Journal of B.U.ON.: Official Journal of the Balkan Union of Oncology
https://www.readbyqxmd.com/read/29134656/long-term-dynamic-synaptic-reorganization-after-gabaergic-precursor-cell-transplantation-into-adult-mouse-spinal-cord
#13
Ida J Llewellyn-Smith, Allan I Basbaum, João M Bráz
Transplanting embryonic precursors of GABAergic neurons from the medial ganglionic eminence (MGE) into adult mouse spinal cord ameliorates mechanical and thermal hypersensitivity in peripheral nerve injury models of neuropathic pain. Although Fos and transneuronal tracing studies strongly suggest that integration of MGE-derived neurons into host spinal cord circuits underlies recovery of function, the extent to which there is synaptic integration of the transplanted cells has not been established. Here, we used electron microscopic immunocytochemistry to assess directly integration of GFP-expressing MGE-derived neuronal precursors into dorsal horn circuitry in intact, adult mice with short- (5-6 weeks) or long-term (4-6 months) transplants...
November 13, 2017: Journal of Comparative Neurology
https://www.readbyqxmd.com/read/29133077/-chronic-kidney-failure-and-biotin-a-combination-inducing-unusual-results-in-thyroid-and-parathyroid-investigations-report-of-2-cases
#14
Miora Koloina Ranaivosoa, Sébastien Ganel, Arnaud Agin, Sarah Romain, Xavier Parent, Nathalie Reix
In clinical chemistry, many immunoassays apply biotin and streptavidin in the assay principle. Presence of high levels of biotin in patient samples can produce negative or positive interference depending on the assay format. In this study, we describe 2 clinical cases with chronic kidney failure and with unusual thyroid and parathyroid function test results due to biotin interference. We studied the impact of biotin levels on thyroid stimulating hormone (TSH), free thyroxine (T4L) and parathormone (PTH) results with a pool of sera loaded with several concentrations of biotin...
November 10, 2017: Néphrologie & Thérapeutique
https://www.readbyqxmd.com/read/29131575/halogenation-of-biotin-protein-ligase-inhibitors-improves-whole-cell-activity-against-staphylococcus-aureus
#15
Ashleigh S Paparella, Kwang Jun Lee, Andrew J Hayes, Jiage Feng, Zikai Feng, Danielle Cini, Sonali Deshmukh, Grant W Booker, Matthew C J Wilce, Steven W Polyak, Andrew D Abell
We report the synthesis and evaluation of 5-halogenated-1,2,3-triazoles as inhibitors of biotin protein ligase from Staphylococcus aureus. The halogenated compounds exhibit significantly improved antibacterial activity over their nonhalogenated counterparts. Importantly, the 5-fluoro-1,2,3-triazole compound 4c displays antibacterial activity against S. aureus ATCC49775 with a minimum inhibitory concentration (MIC) of 8 μg/mL.
November 16, 2017: ACS Infectious Diseases
https://www.readbyqxmd.com/read/29131281/immunohistochemical-study-on-roe-deer-haemal-nodes
#16
Yeşim Akaydın Bozkurt, Ebru Karadağ Sarı, Murat Kabak
The aim of this study was to investigate the immunohistochemical characteristics of the haemal nodes located in the abdominal and the thoracal cavity in roe deer (Capreolus capreolus). In this study, two adult male and two adult female roe deers in addition to two roe deer foetuses at the late fetal stages were used. The avidin-biotin-peroxidase technique was applied to anti-CD3, anti-CD79αcy, anti-macrophage, anti-S100 primary antibodies. Positive results were gained for all the antibodies used. Many CD3 positive T lymphocytes were seen in the lymphoid tissue areas of the fetal haemal nodes, in the germinal center of the lymph follicles of the adult haemal nodes and in their lymphatic cords...
November 13, 2017: Folia Morphologica (Warsz)
https://www.readbyqxmd.com/read/29129284/the-b-vitamin-nutrition-of-insects-the-contributions-of-diet-microbiome-and-horizontally-acquired-genes
#17
REVIEW
Angela E Douglas
Insects generally cannot synthesize eight B vitamins that function as co-enzymes in various required enzymatic reactions. Most insects derive their B vitamin requirements from the diet, microbial symbionts, or a combination of these complementary sources. Exceptionally, the genomes of a few insects bear genes in vitamin B5 (pantothenate) and B7 (biotin) synthesis, horizontally acquired from bacteria. Biomarkers of B vitamin deficiency (e.g. vitamin titers, activity of vitamin-dependent enzymes) offer routes to investigate the incidence and the physiological and fitness consequences of B vitamin deficiency in laboratory and field populations of insects...
October 2017: Current Opinion in Insect Science
https://www.readbyqxmd.com/read/29128715/fabrication-of-ordered-metallic-glass-nanotube-arrays-for-label-free-biosensing-with-diffractive-reflectance
#18
Wei-Ting Chen, Shao-Sian Li, Jinn P Chu, Kuei Chih Feng, Jem-Kun Chen
In this study, a photoresist template with well-defined contact hole array was fabricated, to which radio frequency magnetron sputtering process was then applied to deposit an alloyed Zr55Cu30Al10Ni5 target, and finally resulted in ordered metallic glass nanotube (MGNT) arrays after removal of the photoresist template. The thickness of the MGNT walls increased from 98 to 126nm upon increasing the deposition time from 225 to 675s. The wall thickness of the MGNT arrays also increased while the dimensions of MGNT reduced under the same deposition condition...
October 18, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/29127901/cerium-dioxide-doped-carboxyl-fullerene-as-novel-nanoprobe-and-catalyst-in-electrochemical-biosensor-for-amperometric-detection-of-the-cyp2c19-2-allele-in-human-serum
#19
Chengli Zhang, Junlin He, Yuchan Zhang, Jun Chen, Yilin Zhao, Yazhen Niu, Chao Yu
The disposition dose of clopidogrel is different in CYP2C19*2 gene carriers and non-carriers. High-dose clopidogrel has been recommended to overcome a low-responsiveness to clopidogrel in patients with the CYP2C19*2 gene. To guide the choice of clopidogrel dosage and catalyse a development in the field of personalized therapy, we developed an ultrasensitive electrochemical biosensor to detect CYP2C19*2 gene. We constructed a novel assay based on cerium dioxide (CeO2)-functionalized carboxyl fullerene (c-C60) supported by Pt nanoparticles (c-C60/CeO2/PtNPs) for signal amplification...
November 8, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/29127899/robust-immunosensing-system-based-on-biotin-streptavidin-coupling-for-spatially-localized-femtogram-ml-1-level-detection-of-interleukin-6
#20
Kaixin Zhang, Guozhen Liu, Ewa M Goldys
Detection of a very low amount of cytokines such as interleukin-6 (IL-6) in clinical fluids is important in biomedical research and clinical applications. Here, we demonstrate spatially-localized ultrasensitive (femtogram mL(-1)) level detection of IL-6 in serum and in cell culture media. Our approach is based on a sandwich immunosensor fabricated on the surface of an optical fibre. Firstly, the biotinylated IL-6 capture antibody was immobilized on the fibre surface by biotin-streptavidin coupling. Then the fabricated fibre was used for capturing IL-6 followed by exposure to detection antibody which was labeled with the fluorescent magnetic nanoparticles to report the signal...
November 6, 2017: Biosensors & Bioelectronics
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