keyword
MENU ▼
Read by QxMD icon Read
search

biotin

keyword
https://www.readbyqxmd.com/read/29784356/digital-analysis-with-droplet-based-microfluidic-for-the-ultrasensitive-detection-of-%C3%AE-gal-and-afp
#1
Songbai Tian, Zhen Zhang, Jinyang Chen, Mingyuan Du, Zheng Li, Hai Yang, Xinghu Ji, Zhike He
In this study, we presented a digital analysis with droplet-based microfluidic, which was applied for the detection of β-galactosidase (β-Gal) and AFP. The β-Gal was quantified according to the Poisson Distribution in digital analysis with droplets containing β-Gal and fluorogenic substrate fluorescein di-β-D-galactopyranoside (FDG). In our method, the lowest concentration of β-Gal we could accurately detect was 5 fM. We found that the digital detection could be applied in quantifying protein biomarkers, take AFP for model sample...
August 15, 2018: Talanta
https://www.readbyqxmd.com/read/29783836/a-y-shaped-three-arm-structure-for-probing-bivalent-interactions-between-protein-receptor-ligand-using-afm-and-spr
#2
Subhadip Senapati, Sudipta Biswas, Saikat Manna, Robert Ros, Stuart Lindsay, Peiming Zhang
The goal of this research was to develop linkage chemistry for the study of bivalent interactions between a receptor and its ligand using atomic force microscopy (AFM) and surface plasmon resonance (SPR). We conceived a three-arm structure composed of flexible chains connected to a large rigid core with orthogonal functional groups at their ends for formation and attachment (or immobilization) of bivalent ligands. To demonstrate the principle, we chose the well-known biotin-streptavidin interaction as a model system...
May 21, 2018: Langmuir: the ACS Journal of Surfaces and Colloids
https://www.readbyqxmd.com/read/29779852/treatment-of-progressive-multiple-sclerosis-challenges-and-promising-perspectives
#3
REVIEW
E Maillart
Management of progressive multiple sclerosis (MS) is one of the main challenges of the new century. Based on our knowledge of pathophysiology, three therapeutic strategies are proposed: anti-inflammatory (ocrelizumab, siponimod…); remyelinating (opicinumab); and neuroprotective (high-dose biotin, ibudilast, simvastatin…). Nevertheless, despite recent promising positive clinical trials, new methodological approaches for therapeutic protocols with adaptable outcomes to assess progression are still needed.
May 17, 2018: Revue Neurologique
https://www.readbyqxmd.com/read/29778748/protein-kinases-are-involved-in-the-cardioprotective-effects-activated-by-platelet-glycoprotein-iib-iiia-inhibitor-tirofiban-at-reperfusion-in-rats-in-vivo
#4
Shih-Tai Chang, Ya-Ting Yang, Chi-Ming Chu, Kuo-Li Pan, Jen-Te Hsu, Ju-Feng Hsiao, Yu-Sheng Lin, Chang-Min Chung
The thrombolytic effect of platelet glycoprotein IIb/IIIa inhibitors (GP IIb/IIIa inhibitors) in myocardial infarction has been well established. Nevertheless, data on the mechanism of the cardioprotective effect of GP IIb/IIIa inhibitors in ischemic-reperfusion injury (IR) are lacking. Sprague-Dawley rats received 120min of coronary ischemia and 180min of reperfusion. A GP IIb/IIIa inhibitor was given via continuous intravenous infusion at a rate of 2 ug/kg/min 30min prior to reperfusion with/without inhibitors of PKCε (chelerythrine), PI3 kinase and Akt (wortmannin), p38 MAPK (SB203582), p42/44 MAPK (PD98059) and ERK1/2 (u0126) 15min prior to the GP IIb/IIIa inhibitor...
May 17, 2018: European Journal of Pharmacology
https://www.readbyqxmd.com/read/29772383/a-signal-decreased-electrochemical-immunosensor-for-the-sensitive-detection-of-lag-3-protein-based-on-a-hollow-nanobox-mofs-aupt-alloy
#5
Wei Xu, Zhen Qin, Yutong Hao, Qiang He, Shuai Chen, Zhongshuang Zhang, Dan Peng, Haiyan Wen, Jun Chen, Jingfu Qiu, Chaorui Li
In this work, hollow nanobox metal-organic framework (HNM) nanocomposites were synthesised and utilised for the first time in a signal decreased electrochemical immunosensor for the ultrasensitive quantitative determination of lymphocyte activation gene-3 (LAG-3) protein, which is a newly discovered biomarker. With the aid of signal materials, namely, SiO2 -tagged anti-LAG-3 antibody (SiO2 -Ab2 ) and the biotin-streptavidin system, the sensor can achieve signal amplification. Encapsulation of tin dioxide-functionalised reduced graphene oxide (rGO-SnO2 ) and gold and platinum alloys (AuPt alloys) onto the surface of hollow nanobox metal-organic frameworks (MOFs) was performed to prepare rGO-SnO2 /hollow nanobox-MOFs/AuPt alloys (rGO-SnO2 /HNMs/AuPt) as the matrix...
May 8, 2018: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/29771436/correlations-of-neuronal-apoptosis-with-expressions-of-c-fos-and-c-jun-in-rats-with-post-ischemic-reconditioning-damage
#6
P Xiao, X-W Liu, N-N Zhao, R Fang, Q Wen, K-X Zeng, Y-L Wang
OBJECTIVE: Transcription factors (c-Fos and c-Jun) have been considered to play roles in the initiation of programmed nerve cell death. However, the roles of c-Fos and c-Jun protein expressions in neuronal apoptosis of rats with post-ischemic reconditioning damage were not clarified. Therefore, the aim of this study was to investigate the correlations of protein expressions of c-Fos and c-Jun with neuronal apoptosis of rats with post-ischemic reconditioning damage. MATERIALS AND METHODS: Rat models of post-ischemic reconditioning were established firstly...
May 2018: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/29771132/comparative-diagnostics-of-allergy-using-quantitative-immuno-pcr-and-elisa
#7
Maria A Simonova, Victor D Pivovarov, Dmitry Y Ryazantsev, Anna S Dolgova, Valentina M Berzhets, Sergei K Zavriev, Elena V Svirshchevskaya
AIM: Estimation of specific IgE is essential for the prevention of allergy progression. Quantitative immuno-PCR (qiPCR) can increase the sensitivity of IgE detection. We aimed to develop qiPCR and compare it to the conventional ELISA in identification of IgE to Alt a 1 and Fel d 1 allergens. RESULTS: Single stranded 60-mer DNA conjugated to streptavidin was used to detect antigen-IgE-biotin complex by qiPCR. In semi-logarithmic scale qiPCR data were linear in a full range of serum dilutions resulting in three- to ten-times higher sensitivity of qiPCR in comparison with ELISA in IgE estimation in low titer sera...
May 17, 2018: Bioanalysis
https://www.readbyqxmd.com/read/29770455/development-validation-and-potential-applications-of-biotinylated-red-blood-cells-for-posttransfusion-kinetics-and-other-physiological-studies-evidenced-based-analysis-and-recommendations
#8
REVIEW
Donald M Mock, Demet Nalbant, Svetlana V Kyosseva, Robert L Schmidt, Guohua An, Nell I Matthews, Alexander P J Vlaar, Robin van Bruggen, Dirk de Korte, Ronald G Strauss, José A Cancelas, Robert S Franco, Peter Veng-Pedersen, John A Widness
The current reference method in the United States for measuring in vivo population red blood cell (RBC) kinetics utilizes chromium-51 (51 Cr) RBC labeling for determining RBC volume, 24-hour posttransfusion RBC recovery, and long-term RBC survival. Here we provide evidence supporting adoption of a method for kinetics that uses the biotin-labeled RBCs (BioRBCs) as a superior, versatile method for both regulatory and investigational purposes. RBC kinetic analysis using BioRBCs has important methodologic, analytical, and safety advantages over 51 Cr-labeled RBCs...
May 16, 2018: Transfusion
https://www.readbyqxmd.com/read/29770378/selection-and-characterization-of-a-dna-aptamer-to-crystal-violet
#9
Yang Chen, Jine Wang, Yajie Zhang, Lijun Xu, Tian Gao, Bing Wang, Renjun Pei
Aptamers are short single-stranded DNA or RNA, which can be selected in vitro by systematic evolution of ligands by exponential enrichment (SELEX). In order to develop novel light-up probes to substitute G-quadruplex (G4), we selected a DNA aptamer for crystal violet (CV), a triphenylmethane light-up dye, by a modified affinity chromatography-based SELEX. The ssDNA pool was first coupled on streptavidin-coated agarose beads through a biotin labeled complementary oligonucleotide, and then the aptamer sequences would be released from agarose beads by CV affinity...
May 17, 2018: Photochemical & Photobiological Sciences
https://www.readbyqxmd.com/read/29770345/biotin-thiamine-responsive-basal-ganglia-disease-case-report-and-follow-up-of-a-patient-with-poor-compliance
#10
Muneera A Alabdulqader, Sumayah Al Hajjaj
Background: Biotin-thiamine-responsive basal ganglia disease (BTBGD) is a rare treatable autosomal recessive neurometabolic disorder characterized by progressive encephalopathy that eventually leads to severe disability and death if not treated with biotin and thiamine supplements. Objectives: We aimed to determine the optimal management of BTBGD presenting in acute encephalopathic episodes. Method: Case report. Results: An 8-year-old girl born to consanguineous parents was diagnosed with BTBGD at the age of 3 years after presenting with acute encephalopathy and ataxia...
2018: Child Neurology Open
https://www.readbyqxmd.com/read/29769518/a-cell-penetrating-artificial-metalloenzyme-regulates-a-gene-switch-in-a-designer-mammalian-cell
#11
Yasunori Okamoto, Ryosuke Kojima, Fabian Schwizer, Eline Bartolami, Tillmann Heinisch, Stefan Matile, Martin Fussenegger, Thomas R Ward
Complementing enzymes in their native environment with either homogeneous or heterogeneous catalysts is challenging due to the sea of functionalities present within a cell. To supplement these efforts, artificial metalloenzymes are drawing attention as they combine attractive features of both homogeneous catalysts and enzymes. Herein we show that such hybrid catalysts consisting of a metal cofactor, a cell-penetrating module, and a protein scaffold are taken up into HEK-293T cells where they catalyze the uncaging of a hormone...
May 16, 2018: Nature Communications
https://www.readbyqxmd.com/read/29769406/spindle-assembly-disruption-and-cancer-cell-apoptosis-with-a-cltc-binding-compound
#12
Michael J Bond, Marina Bleiler, Lauren E Harrison, Eric W Scocchera, Masako Nakanishi, Narendran G-Dayanandan, Santosh Keshipeddy, Daniel W Rosenberg, Dennis L Wright, Charles Giardina
AK3 compounds are mitotic-arrest agents that induce high levels of γH2AX during mitosis and apoptosis following release from arrest. We synthesized a potent AK3 derivative, AK306, that induced arrest and apoptosis of the HCT116 colon cancer cell line with an EC50 of ~50 nM. AK306 was active on a broad spectrum of cancer cell lines with total growth inhibition values ranging from ~25 nM to 25 µM. Using biotin and BODIPY-linked derivatives of AK306, binding to clathrin heavy chain (CLTC/CHC) was observed, a protein with roles in endocytosis and mitosis...
May 16, 2018: Molecular Cancer Research: MCR
https://www.readbyqxmd.com/read/29768434/plasma-membrane-insertion-of-kca2-3-sk3-is-dependent-upon-the-snare-proteins-syntaxin-4-and-snap23
#13
Claudia A Bertuccio, Tony T Wang, Kirk L Hamilton, Diego J Rodriguez-Gil, Steven B Condliffe, Daniel C Devor
We previously demonstrated endocytosis of KCa2.3 is caveolin-1-, dynamin II- and Rab5-dependent. KCa2.3 then enters Rab35/EPI64C- and RME-1-containing recycling endosomes and is returned to the plasma membrane (PM). Herein, we report on the mechanism by which KCa2.3 is inserted into the PM during recycling and following exit from the Golgi. We demonstrate KCa2.3 colocalizes with SNAP-23 and Syntaxin-4 in the PM of HEK and endothelial cells by confocal immunofluorescence microscopy. We further show KCa2.3 can be co-immunoprecipitated with SNAP-23 and Syntaxin-4...
2018: PloS One
https://www.readbyqxmd.com/read/29768001/crystal-structures-of-the-mango-ii-rna-aptamer-reveal-heterogeneous-fluorophore-binding-and-guide-engineering-of-variants-with-improved-selectivity-and-brightness
#14
Robert Trachman, Amir Abdolahzadeh, Alessio Andreoni, Razvan Cojocaru, Jay R Knutson, Michaël Ryckelynck, Peter John Unrau, Adrian Ferré-D'Amaré
Several RNA aptamers that bind small molecules and enhance their fluorescence have been successfully used to tag and track RNAs in vivo, but these genetically encodable tags have not yet achieved single-fluorophore resolution. Recently, Mango-II, an RNA that binds TO1-Biotin with ~1 nM affinity and enhances its fluorescence by >1,500-fold was isolated by fluorescence selection from the pool that yielded the original RNA Mango. We determined the crystal structures of Mango-II in complex with two fluorophores, TO1-Biotin and TO3-Biotin, and found that despite their high affinity, the ligands adopt multiple distinct conformations, indicative of a binding pocket with modest stereoselectivity...
May 16, 2018: Biochemistry
https://www.readbyqxmd.com/read/29767664/biochemical-and-molecular-characterization-of-3-methylcrotonylglycinuria-in-an-italian-asymptomatic-girl
#15
Carla Cozzolino, Guglielmo Rd Villani, Giulia Frisso, Emanuela Scolamiero, Lucia Albano, Giovanna Gallo, Roberta Romanelli, Margherita Ruoppolo
3-Methylcrotonylglycinuria is an organic aciduria resulting from deficiency of 3-methylcrotonyl-CoA carboxylase (3-MCC), a biotin-dependent mitochondrial enzym carboxylating 3-methylcrotonyl-CoA to 3-methylglutaconyl-CoA during leucine catabolism. Its deficiency, due to mutations on MCCC1 and MCCC2 genes, leads to accumulation of 3-methylcrotonyl-CoA metabolites in blood and/or urine, primarily 3-hydroxyisovaleryl-carnitine (C5-OH) in plasma and 3-methylcrotonyl-glycine (3-MCG) and 3-hydroxyisovaleric acid (3-HIVA) in the urine...
May 14, 2018: Genetics and Molecular Biology
https://www.readbyqxmd.com/read/29766001/production-of-recombinant-streptavidin-and-optimization-of-refolding-conditions-for-recovery-of-biological-activity
#16
Raoufe Modanloo Jouybari, Abdorrahim Sadeghi, Behzad Khansarinejad, Shabnam Sadoogh Abbasian, Hamid Abtahi
Background: Streptavidin is a protein produced by Streptomyces avidinii with strong biotin-binding ability. The non-covalent, yet strong bond between these two molecules has made it a preferable option in biological detection systems. Due to its extensive use, considerable attention is focused on streptavidin production by recombinant methods. Methods: In this study, streptavidin was expressed in Escherichia coli (E. coli) BL21 (DE3) pLysS cells and purified by affinity chromatography...
April 2018: Reports of Biochemistry & Molecular Biology
https://www.readbyqxmd.com/read/29762848/cxcr3-mediates-chondrocyte-injury-through-regulating-nitric-oxide
#17
Z-C Yin, W-H Xiong, Q-J Pang
OBJECTIVE: As a common joint disease, osteoarthritis exhibits increasing trend in recent years. C-X-C motif chemokine receptor 3 (CXCR3) is a kind of chemokine with the characteristic of recruiting inflammatory cells. Its function in osteoarthritis has not been clarified. This study aims to explore the role of CXCR3 in cartilage injury by affecting unfolded protein response (UPR) pathway. PATIENTS AND METHODS: The sample was obtained from osteoarthritis patients to test CXCR3 expression by Real-time polymerase chain reaction (PCR)...
April 2018: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/29760774/rich-biotin-content-in-lignocellulose-biomass-plays-the-key-role-in-determining-cellulosic-glutamic-acid-accumulation-by-corynebacterium-glutamicum
#18
Jingbai Wen, Yanqiu Xiao, Ting Liu, Qiuqiang Gao, Jie Bao
Background: Lignocellulose is one of the most promising alternative feedstocks for glutamic acid production as commodity building block chemical, but the efforts by the dominant industrial fermentation strain Corynebacterium glutamicum failed for accumulating glutamic acid using lignocellulose feedstock. Results: We identified the existence of surprisingly high biotin concentration in corn stover hydrolysate as the determining factor for the failure of glutamic acid accumulation by Corynebacterium glutamicum ...
2018: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/29760259/biotin-interference-underrecognized-patient-safety-risk-in-laboratory-testing
#19
Jessica L Gifford, S M Hossein Sadrzadeh, Christopher Naugler
No abstract text is available yet for this article.
May 2018: Canadian Family Physician Médecin de Famille Canadien
https://www.readbyqxmd.com/read/29759175/gold-nanoparticle-aggregation-colorimetric-detection-of-the-interactions-between-avidin-and-biotin
#20
Dongmin Shi, Feifan Sheng, Xiaojun Zhang, Guangfeng Wang
This paper reported a novel colorimetric assay strategy for avidin and biotin interactions based on terminal protection of the biotinylated single-stranded DNA and the surface plasmon resonance adsorption of gold nanoparticles (AuNPs). In this assay, it was firstly found that biotin-ssDNA specifically bound to the target protein avidin with strong affinity could be protected from hydrolysis by exonuclease I (Exo I). Furthermore, a colorimetric strategy was designed for the detection of avidin and biotin interactions...
August 1, 2018: Talanta
keyword
keyword
37252
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"