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https://www.readbyqxmd.com/read/29334159/a-gold-iii-pincer-ligand-scaffold-for-the-synthesis-of-binuclear-and-bioconjugated-complexes-synthesis-and-anticancer-potential
#1
Bertrand Benoît, Maria A O'Connell, Zöe A E Waller, Manfred Bochmann
Cyclometalated (C^N^C)Au(III) complexes bearing functionalized N-heterocyclic carbene (NHC) ligands provide a high-yielding, modular route to bioconjugated and binuclear complexes. That methodology has been applied to the synthesis of bioconjugated complexes presenting biotin and 17α-ethynylestradiol vectors, as well as to the synthesis of bimetallic Au(III)/Au(I) complexes. The in vitro antiproliferative activities of these compounds against various cancer cells lines depend on the linker length, with the longer linker being the most potent...
January 15, 2018: Chemistry: a European Journal
https://www.readbyqxmd.com/read/29331427/water-soluble-mercury-ion-sensing-based-on-the-thymine-hg2-thymine-base-pair-using-retroreflective-janus-particle-as-an-optical-signaling-probe
#2
Hyeong Jin Chun, Saemi Kim, Yong Duk Han, Dong Woo Kim, Ka Ram Kim, Hyo-Sop Kim, Jae-Ho Kim, Hyun C Yoon
Herein, we report an optical sensing platform for mercury ions (Hg2+) in water based on the integration of Hg2+-mediated thymine-thymine (T-T) stabilization, a biotinylated stem-loop DNA probe, and a streptavidin-modified retroreflective Janus particle (SA-RJP). Two oligonucleotide probes, including a stem-loop DNA probe and an assistant DNA probe, were utilized. In the absence of Hg2+, the assistant DNA probe does not hybridize with the stem-loop probe due to their T-T mismatch, so the surface-immobilized stem-loop DNA probe remains a closed hairpin structure...
January 6, 2018: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/29331424/ultra-narrow-surface-lattice-resonances-in-plasmonic-metamaterial-arrays-for-biosensing-applications
#3
Artem Danilov, Gleb Tselikov, Fan Wu, Vasyl G Kravets, Igor Ozerov, Frederic Bedu, Alexander N Grigorenko, Andrei V Kabashin
When excited over a periodic metamaterial lattice of gold nanoparticles (~ 100nm), localized plasmon resonances (LPR) can be coupled by a diffraction wave propagating along the array plane, which leads to a drastic narrowing of plasmon resonance lineshapes (down to a few nm full-width-at-half-maximum) and the generation of singularities of phase of reflected light. These phenomena look very promising for the improvement of performance of plasmonic biosensors, but conditions of implementation of such diffractively coupled plasmonic resonances, also referred to as plasmonic surface lattice resonances (PSLR), are not always compatible with biosensing arrangement implying the placement of the nanoparticles between a glass substrate and a sample medium (air, water)...
December 9, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/29330181/a-novel-l-glutamate-exporter-of-corynebacterium-glutamicum
#4
Yu Wang, Guoqiang Cao, Deyu Xu, Liwen Fan, Xinyang Wu, Xiaomeng Ni, Shuxin Zhao, Ping Zheng, Jibin Sun, Yanhe Ma
Besides metabolic pathways and regulatory networks, transport systems are also pivotal for cellular metabolism and hyper-production of biochemicals using microbial cell factories. Identification and characterization of transporters are therefore of great significance for understanding and engineering of transport reactions. Herein, a novel l-glutamate exporter MscCG2 that extensively exists in Corynebacterium glutamicum strains but is distinct from the only known l-glutamate exporter MscCG was discovered in an industrial l-glutamate producing C...
January 12, 2018: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/29328971/ultrasensitive-electrochemical-sensing-of-hg2-based-on-thymine-hg2-thymine-interaction-and-signal-amplification-of-alkaline-phosphatase-catalyzed-silver-deposition
#5
Aigui Xu, Long Chao, Hongbo Xiao, Yuyun Sui, Jia Liu, Qingji Xie, Shouzhuo Yao
We report an ultrasensitive electrochemical sensor for Hg2+ detection, on the basis of two Hg2+-specific oligonucleotide probes (a thiolated capture probe and a biotinated signal probe), "terminal" signal amplification of alkaline phosphatase catalyzed deposition of silver and in situ microliter-droplet anodic stripping voltammetry (ASV). The thiolated capture probe is immobilized on an Au-plated glassy carbon electrode, and the biotinated signal probe is then attached to the electrode surface through the thymine-Hg2+-thymine interaction in the presence of Hg2+...
January 5, 2018: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/29327918/zeptomole-detection-scheme-based-on-levitation-coordinate-measurements-of-a-single-microparticle-in-a-coupled-acoustic-gravitational-field
#6
Akihisa Miayagawa, Makoto Harada, Tetsuo Okada
We present a novel analytical principle in which an analyte (according to its concentration) induces a change in the density of a microparticle, which is measured as a vertical coordinate in a coupled acoustic-gravitational (CAG) field. The density change is caused by the binding of gold nanoparticles (AuNPs) on a polystyrene (PS) microparticle through avidin-biotin association. The density of a 10-m PS particle increases by 2 % when 500 100-nm AuNPs are bound to the PS. The CAG can detect this density change as a 5-10-m shift of the levitation coordinate of the PS...
January 12, 2018: Analytical Chemistry
https://www.readbyqxmd.com/read/29325213/a-new-colorimetric-platform-for-protein-detection-based-on-recognition-induced-cascade-of-polymeric-nanoparticles-disassembly
#7
Fang Fang, Jin Liu, Yushu Li, Junjiao Yang, Jing Yang
Despite great progress, it is still of high interest to explore new homogeneous assays for simple, visual, and selective protein detection. Herein, one new colorimetric sensor has been developed for visual detection of protein by using polymeric micelles as a sensing scaffold and the molecular recognition between protein and the ligand on the surface of the polymeric micelles as the driving force to trigger the readout of the detection signal. The polymeric micelles formed via the self-assembly of the amphiphilic block polymer biotin-labeled poly(ethylene glycol)-block-poly(3-acryl aminophenylboronic acid) are endowed with colorful feature by incorporation of alizarin red S (ARS) into the hydrophobic core...
January 11, 2018: Macromolecular Bioscience
https://www.readbyqxmd.com/read/29324648/lspr-and-interferometric-sensor-modalities-combined-using-a-double-clad-optical-fiber
#8
Harald Ian Muri, Andon Bano, Dag Roar Hjelme
We report on characterization of an optical fiber-based multi-parameter sensor concept combining localized surface plasmon resonance (LSPR) signal and interferometric sensing using a double-clad optical fiber. The sensor consists of a micro-Fabry-Perot in the form of a hemispherical stimuli-responsive hydrogel with immobilized gold nanorods on the facet of a cleaved double-clad optical fiber. The swelling degree of the hydrogel is measured interferometrically using the single-mode inner core, while the LSPR signal is measured using the multi-mode inner cladding...
January 11, 2018: Sensors
https://www.readbyqxmd.com/read/29324341/biotin-conjugated-organic-molecules-and-proteins-for-cancer-therapy-a-review
#9
Santanu Maiti, Priyankar Paira
The main transporter for biotin is sodium dependent multivitamin transporter (SMVT), which is overexpressed in various aggressive cancer cell lines such as ovarian (OV 2008, ID8), leukemia (L1210FR), mastocytoma (P815), colon (Colo-26), breast (4T1, JC, MMT06056), renal (RENCA, RD0995), and lung (M109) cancer cell lines. Furthermore, its overexpression was found higher to that of folate receptor. Therefore, biotin demand in the rapidly growing tumors is higher than normal tissues. Several biotin conjugated organic molecules has been reported here for selective delivery of the drug in cancer cell...
January 2, 2018: European Journal of Medicinal Chemistry
https://www.readbyqxmd.com/read/29323388/a-novel-thermal-detection-method-based-on-molecularly-imprinted-nanoparticles-as-recognition-elements
#10
Francesco Canfarotta, J Czulak, K Betlem, A Sachdeva, K Eersels, B van Grinsven, T J Cleij, M Peeters
Molecularly Imprinted Polymers (MIPs) are synthetic receptors that are able to selectively bind their target molecule and, for this reason, they are currently employed as recognition elements in sensors. In this work, MIP nanoparticles (nanoMIPs) are produced by solid-phase synthesis for a range of templates with different sizes, including a small molecule (biotin), two peptides (one derived from the epithelial growth factor receptor and vancomycin) and a protein (trypsin). NanoMIPs are then dipcoated on the surface of thermocouples that measure the temperature inside a liquid flow cell...
January 11, 2018: Nanoscale
https://www.readbyqxmd.com/read/29318873/a-mass-producible-and-versatile-sensing-system-localized-surface-plasmon-resonance-excited-by-individual-waveguide-modes
#11
Zhutian Ding, James M Stubbs, Danielle McRae, Johanna M Blacquiere, François Lagugné-Labarthet, Silvia Mittler
A plasmonic sensing system that allows the excitation of localized surface plasmon resonance (LSPR) by individual waveguide modes is presented conceptually and experimentally. Any change in the local environment of the gold nanoparticles (AuNPs) alters the degree of coupling between LSPR and a polymer slab waveguide, which then modulates the transmission-output signal. In comparison to conventional LSPR sensors, this system is less susceptible to optical noise and positional variation of signals. Moreover, it enables more freedom in the exploitation of plasmonic hot spots with both transverse electric (TE) and transverse magnetic (TM) modes...
January 10, 2018: ACS Sensors
https://www.readbyqxmd.com/read/29318541/biotin-chasing-assay-to-evaluate-upar-stability-and-cleavage-on-the%C3%A2-surface-of-cells
#12
Vladimir Leksa, Herbert B Schiller, Hannes Stockinger
The plasminogen activation system, i.e., the fibrinolytic system, is one of the major plasma proteolytic pathways. The proteolytic conversion of the zymogen plasminogen to the active serine protease plasmin is on the cell surface catalyzed by the serine protease urokinase-type plasminogen activator (urokinase, uPA). Upon binding to the urokinase receptor (uPAR, CD87), single-chain pro-uPA is processed to double-chain uPA which in turn specifically converts cell-bound plasminogen to plasmin. Plasmin is harnessed in many physiological processes, e...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29314108/high-dose-biotin-in-infants-mimics-biochemical-hyperthyroidism-with-some-commercial-assays
#13
Ved Bhushan Arya, Michal Ajzensztejn, Gayle Appleby, Sue Oddy, David Halsall, Krishna Chatterjee, Carla Moran, Tony Hulse
Biotin is a water-soluble B-complex vitamin, which is an essential coenzyme for four carboxylases enzymes involved in intermediary metabolism. Although the recommended daily intake (RDI) is 30 micrograms, doses as high as 100 times the RDI are widely used for dietary supplementation, therapy of several inherited metabolic diseases (biotin-thiamine responsive basal ganglia disease and biotinidase deficiency) and supportive treatment in patients with disorders of mitochondrial energy metabolism. This article is protected by copyright...
January 5, 2018: Clinical Endocrinology
https://www.readbyqxmd.com/read/29313597/vitamin-d-and-vitamin-d-receptor-in-patients-with-ophthalmic-pterygium
#14
Cristina Maxia, Daniela Murtas, Michela Corrias, Ignazio Zucca, Luigi Minerba, Franca Piras, Cristiana Marinelli, Maria Teresa Perra
Pterygium, an ultraviolet radiation (UV)-related disease, is a relatively benign process, but since it displays tumor-like features, it has been proposed to be a neoplastic- like growth disorder. Vitamin D performs a number of functions in addition to calcium homeostasis, as inhibition of cell proliferation, activation of apoptotic pathways, and inhibition of angiogenesis. Since the antitumor actions of vitamin D are mediated primarily through the nuclear vitamin D receptor (VDR), the aim of the present study was to investigate vitamin D status in patients with pterygium and in control subjects, and VDR immunohistochemical expression in samples of pterygium and normal conjunctiva in order to evaluate a possible role of vitamin D pathway in the pathogenesis of the disease...
October 30, 2017: European Journal of Histochemistry: EJH
https://www.readbyqxmd.com/read/29311743/tdp-43-pathology-disrupts-nuclear-pore-complexes-and-nucleocytoplasmic-transport-in-als-ftd
#15
Ching-Chieh Chou, Yi Zhang, Mfon E Umoh, Spencer W Vaughan, Ileana Lorenzini, Feilin Liu, Melissa Sayegh, Paul G Donlin-Asp, Yu Han Chen, Duc M Duong, Nicholas T Seyfried, Maureen A Powers, Thomas Kukar, Chadwick M Hales, Marla Gearing, Nigel J Cairns, Kevin B Boylan, Dennis W Dickson, Rosa Rademakers, Yong-Jie Zhang, Leonard Petrucelli, Rita Sattler, Daniela C Zarnescu, Jonathan D Glass, Wilfried Rossoll
The cytoplasmic mislocalization and aggregation of TAR DNA-binding protein-43 (TDP-43) is a common histopathological hallmark of the amyotrophic lateral sclerosis and frontotemporal dementia disease spectrum (ALS/FTD). However, the composition of aggregates and their contribution to the disease process remain unknown. Here we used proximity-dependent biotin identification (BioID) to interrogate the interactome of detergent-insoluble TDP-43 aggregates and found them enriched for components of the nuclear pore complex and nucleocytoplasmic transport machinery...
January 8, 2018: Nature Neuroscience
https://www.readbyqxmd.com/read/29306770/development-of-a-new-enzyme-immunoassay-for-improved-detection-of-rotavirus-in-fecal-specimens-of-vaccinated-infants
#16
Houping Wang, Merry Liu, Ken Sugata, Yuhuan Wang, Jennifer Hull, Kimberly Foytich, Baoming Jiang
BACKGROUND: Group A rotavirus is the most common cause of acute diarrhea in young children worldwide. A simple and rapid enzyme immunoassay (EIA) has been commonly used to detect rotavirus infection and evaluate rotavirus vaccines. Currently licensed commercial EIA kits have low sensitivity. A more sensitive detection of rotavirus can improve rotavirus diagnostics and vaccine efficacy studies. OBJECTIVE: A biotin-avidin based sandwich EIA was developed and compared with commercial EIA kits for improved detection of viral shedding in fecal samples from infants who received human rotavirus vaccine Rotarix in Mexico...
December 26, 2017: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://www.readbyqxmd.com/read/29306572/novel-biotin-linker-with-alkyne-and-amino-groups-for-chemical-labelling-of-a-target-protein-of-a-bioactive-small-molecule
#17
Tomoaki Anabuki, Miu Tsukahara, Masanori Okamoto, Hideyuki Matsuura, Kosaku Takahashi
We synthesized a novel linker (1) with biotin, alkyne and amino groups for the identification of target proteins using a small molecule that contains an azide group (azide probe). The alkyne in the linker bound the azide probe via an azide-alkyne Huisgen cycloaddition. A protein cross-linker effectively bound the conjugate of the linker and an azide probe with a target protein. The covalently bound complex was detected by western blotting. Linker 1 was applied to a model system using an abscisic acid receptor, RCAR/PYR/PYL (PYL)...
December 26, 2017: Bioorganic & Medicinal Chemistry Letters
https://www.readbyqxmd.com/read/29305188/biotin-decorated-anti-cancer-nucleotide-theranostic-conjugate-of-human-serum-albumin-where-the-seed-meets-the-soil
#18
Tatyana V Popova, Hamda Khan, Alexey S Chubarov, Vladimir A Lisitskiy, Natalya M Antonova, Andrey E Akulov, Oleg B Shevelev, Evgenii L Zavjalov, Vladimir N Silnikov, Saheem Ahmad, Tatyana S Godovikova
Human serum albumin is playing an increasing role as a drug carrier in clinical settings. Biotin molecules are often used as suitable tags in targeted anti-tumor drug delivery systems. We report on the synthesis and properties of a new multimodal theranostic conjugate based on an anti-cancer fluorinated nucleotide conjugated with a biotinylated dual-labeled albumin. Interestingly, in vitro and in vivo study revealed stronger anti-tumor activity of the non-tagged theranostic conjugate than that of the biotin-tagged conjugate, which can be explained by decreased binding of the biotin-tagged conjugate to cellular receptors...
December 26, 2017: Bioorganic & Medicinal Chemistry Letters
https://www.readbyqxmd.com/read/29303768/detection-of-autoantibodies-to-the-p200-epitope-of-ssa-ro52-antigen-a-comparison-of-two-laboratory-assays
#19
Elena Mattia, Ariela Hoxha, Marta Tonello, Maria Favaro, Teresa Del Ross, Antonia Calligaro, Anna Ghirardello, Amelia Ruffatti
BACKGROUND: Anti-p200 antibodies have been receiving growing interest in view of findings associating their presence to risk of fetal autoimmune congenital heart block (CHB). The study compares and evaluates the performance of two assays currently being used for their detection. METHODS: One hundred and sixteen pregnant women positive for anti-SSA/Ro52 antibodies were considered as the study population. Fifty women negative for anti-SSA/Ro52 antibodies were considered as the control population...
January 5, 2018: Clinical Chemistry and Laboratory Medicine: CCLM
https://www.readbyqxmd.com/read/29303200/a-modular-approach-for-multifunctional-polymersomes-with-controlled-adhesive-properties
#20
Julien Petit, Laura Thomi, Jennifer Schultze, Marcin Makowski, Inka Negwer, Kaloian Koynov, Stephan Herminghaus, Frederik R Wurm, Oliver Bäumchen, Katharina Landfester
The bottom-up approach in synthetic biology involves the engineering of synthetic cells by designing biological and chemical building blocks, which can be combined in order to mimic cellular functions. The first step for mimicking a living cell is the design of an appropriate compartment featuring a multifunctional membrane. This is of particular interest since it allows for the selective attachment of different groups or molecules to the membrane. In this context, we report on a modular approach for polymeric vesicles, so-called polymersomes, with a multifunctional surface, namely hydroxyl, alkyne and acrylate groups...
January 5, 2018: Soft Matter
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