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Histone H4

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https://www.readbyqxmd.com/read/28424421/the-soy-derived-peptide-lunasin-inhibits-invasive-potential-of-melanoma-initiating-cells
#1
Chris Shidal, Jun-Ichi Inaba, Kavitha Yaddanapudi, Keith R Davis
Lunasin is a 44 amino acid peptide with multiple functional domains including an aspartic acid tail, an RGD domain, and a chromatin-binding helical domain. We recently showed that Lunasin induced a phenotype switch of cancer initiating cells (CIC) out of the stem compartment by inducing melanocyte-associated differentiation markers while simultaneously reducing stem-cell-associated transcription factors. In the present study, we advance the hypothesis that Lunasin can reduce pools of melanoma cells with stem cell-like properties, and demonstrate that Lunasin treatment effectively inhibits the invasive potential of CICs in vitro as well as in vivo in a mouse experimental metastasis model...
March 10, 2017: Oncotarget
https://www.readbyqxmd.com/read/28421257/histone-deacetylase-inhibitors-reverse-age-related-increases-in-side-effects-of-haloperidol-in-mice
#2
Janitza L Montalvo-Ortiz, Daniel W Fisher, Guadalupe Rodríguez, Deyu Fang, John G Csernansky, Hongxin Dong
BACKGROUND: Older patients can be especially susceptible to antipsychotic-induced side effects, and the pharmacodynamic mechanism underlying this phenomenon remains unclear. We hypothesized that age-related epigenetic alterations lead to decreased expression and functionality of the dopamine D2 receptor (D2R), contributing to this susceptibility. METHODS: In this study, we treated young (2-3 months old) and aged (22-24 months old) C57BL/6 mice with the D2R antagonist haloperidol (HAL) once a day for 14 days to evaluate HAL-induced motor side effects...
April 18, 2017: Psychopharmacology
https://www.readbyqxmd.com/read/28418026/the-cac2-subunit-is-essential-for-productive-histone-binding-and-nucleosome-assembly-in-caf-1
#3
Francesca Mattiroli, Yajie Gu, Jeremy L Balsbaugh, Natalie G Ahn, Karolin Luger
Nucleosome assembly following DNA replication controls epigenome maintenance and genome integrity. Chromatin assembly factor 1 (CAF-1) is the histone chaperone responsible for histone (H3-H4)2 deposition following DNA synthesis. Structural and functional details for this chaperone complex and its interaction with histones are slowly emerging. Using hydrogen-deuterium exchange coupled to mass spectrometry, combined with in vitro and in vivo mutagenesis studies, we identified the regions involved in the direct interaction between the yeast CAF-1 subunits, and mapped the CAF-1 domains responsible for H3-H4 binding...
April 18, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28415063/neuron-restrictive-silencer-factor-mediated-downregulation-of-%C3%AE-opioid-receptor-contributes-to-the-reduced-morphine-analgesia-in-bone-cancer-pain
#4
Chao Zhu, Jun Tang, Tan Ding, Lei Chen, Wei Wang, Xiao-Peng Mei, Xiao-Tao He, Wen Wang, Li-Dong Zhang, Yu-Lin Dong, Zhuo-Jing Luo
Bone cancer pain has been reported to have unique mechanisms and is resistant to morphine treatment. Recent studies have indicated that neuron-restrictive silencer factor (NRSF) plays a crucial role in modulating the expression of the μ-opioid receptor (MOR) gene. The present study elucidates the regulatory mechanisms of MOR and its ability to affect bone cancer pain. Using a sarcoma-inoculated murine model, pain behaviors that represent continuous or breakthrough pain were evaluated. Expression of NRSF in the dorsal root ganglion (DRG) and spinal dorsal horn was quantified at the transcriptional and translational levels, respectively...
May 2017: Pain
https://www.readbyqxmd.com/read/28407300/thrombin-induces-ccl2-expression-in-human-lung-fibroblasts-via-p300-mediated-histone-acetylation-and-nf-kappab-activation
#5
Xiaoling Deng, Xiaoqiong Zhou, Yan Deng, Fan Liu, Xiaofan Feng, Qi Yin, Yinzhen Gu, Songlin Shi, Mingyan Xu
BACKGROUND AND OBJECTIVE: Thrombin has been shown to play a key role in lung diseases such as pulmonary fibrosis via the induction of fibrotic cytokine- chemokine (CC motif) ligand-2 (CCL2) expression. We previously reported that transcription factor nuclear factor-κB (NF-κB) is responsible for thrombin-induced CCL2 expression in human lung fibroblasts (HLFs). Here we extended our study to investigate the epigenetic regulation mechanism for thrombin-induced CCL2 expression in HLF. METHODS: HLFs were cultured in F-12 medium...
April 13, 2017: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/28404794/neprosin-a-selective-prolyl-endoprotease-for-bottom-up-proteomics-and-histone-mapping
#6
Christoph U Schraeder, Linda Lee, Martial Rey, Vladimir Sarpe, Petr Man, Seema Sharma, Vlad Zabrouskov, Brett Larsen, David C Schriemer
Trypsin dominates bottom-up proteomics, but there are reasons to consider alternative enzymes. Improving sequence coverage, exposing proteomic dark matter and clustering post-translational modifications in different ways and with higher-order drive the pursuit of reagents complementary to trypsin. Additionally, enzymes that are easy to use and generate larger peptides that capitalize upon newer fragmentation technologies should have a place in proteomics. We expressed and characterized recombinant neprosin, a novel prolyl endoprotease of the DUF239 family, which preferentially cleaves C-terminal to proline residues under highly acidic conditions...
April 12, 2017: Molecular & Cellular Proteomics: MCP
https://www.readbyqxmd.com/read/28402630/benzo-isoquinoline-diones-as-potent-and-selective-inhibitors-of-brpf2-and-taf1-taf1l-bromodomains
#7
Léa Bouché, Clara D Christ, Stephan Siegel, Amaury E Fernández-Montalván, Simon J Holton, Oleg Fedorov, Antonius Ter Laak, Tatsuo Sugawara, Detlef Stöckigt, Cynthia Tallant, James M Bennett, Octovia P Monteiro, Laura Díaz-Sáez, Paulina Siejka, Julia Meier, Vera Puetter, Joerg Weiske, Susanne Müller, Kilian Veit Marcus Huber, Ingo V Hartung, Bernard Haendler
Bromodomains (BD) are readers of lysine acetylation marks present in numerous proteins associated with chromatin. Here we describe a dual inhibitor of the bromodomain and PHD finger (BRPF) family member BRPF2 and the TATA box binding protein-associated factors TAF1 and TAF1L. These proteins are found in large chromatin complexes and play important roles in transcription regulation. The substituted benzo-isoquinoline-dione series was identified by high-througput screening and subsequent structure-activity relationship optimization allowed generation of low nanomolar BRPF2 BD inhibitors with strong selectivity against BRPF1 and BRPF3 BDs...
April 12, 2017: Journal of Medicinal Chemistry
https://www.readbyqxmd.com/read/28396698/internal-modifications-in-the-cenp-a-nucleosome-modulate-centromeric-dynamics
#8
Minh Bui, Mary Pitman, Arthur Nuccio, Serene Roque, Paul Gregory Donlin-Asp, Aleksandra Nita-Lazar, Garegin A Papoian, Yamini Dalal
BACKGROUND: Posttranslational modifications of core histones are correlated with changes in transcriptional status, chromatin fiber folding, and nucleosome dynamics. However, within the centromere-specific histone H3 variant CENP-A, few modifications have been reported, and their functions remain largely unexplored. In this multidisciplinary report, we utilize in silico computational and in vivo approaches to dissect lysine 124 of human CENP-A, which was previously reported to be acetylated in advance of replication...
2017: Epigenetics & Chromatin
https://www.readbyqxmd.com/read/28392790/hist1h1c-regulates-interferon-%C3%AE-and-inhibits-influenza-virus-replication-by-interacting-with-irf3
#9
Xiaokun Liu, Cha Yang, Yong Hu, Erming Lei, Xian Lin, Lianzhong Zhao, Zhong Zou, Anding Zhang, Hongbo Zhou, Huanchun Chen, Ping Qian, Meilin Jin
Influenza virus NS2 is well known for its role in viral ribonucleoprotein nuclear export; however, its function has not been fully understood. A recent study showed that NS2 might interact with HIST1H1C (H1C, H1.2). Histones have been found to affect influenza virus replication, such as the H2A, H2B, H3, and H4, but H1 has not been detected. Here, we found that H1C interacts with NS2 via its C-terminal in the nucleus and that H1C affects influenza virus replication. The H1N1 influenza virus replicates better in H1C knockout A549 cells compared to wild-type A549 cells, primarily because of the regulation of H1C on interferon-β (IFN-β)...
2017: Frontiers in Immunology
https://www.readbyqxmd.com/read/28389581/tssk6-is-required-for-%C3%AE-h2ax-formation-and-the-histone-to-protamine-transition-during-spermiogenesis
#10
Kula N Jha, Swamy K Tripurani, Gibbes R Johnson
Spermiogenesis includes transcriptional silencing, chromatin condensation and extensive morphological changes as spermatids transform into sperm. Chromatin condensation involves histone hyperacetylation, transitory DNA breaks, histone H2AX Ser139 phosphorylation (γH2AX), and replacement of histones by protamines. Previously, we reported that the spermatid protein kinase TSSK6 is essential for fertility in mice, but its specific role in spermiogenesis is unknown. Here, we show that TSSK6 expression is spatiotemporally coincident with γH2AX formation in nuclei of developing mouse spermatids...
April 7, 2017: Journal of Cell Science
https://www.readbyqxmd.com/read/28389464/chromatin-determinants-impart-camptothecin-sensitivity
#11
Fabio Puddu, Israel Salguero, Mareike Herzog, Nicola J Geisler, Vincenzo Costanzo, Stephen P Jackson
Camptothecin-induced locking of topoisomerase 1 on DNA generates a physical barrier to replication fork progression and creates topological stress. By allowing replisome rotation, absence of the Tof1/Csm3 complex promotes the conversion of impending topological stress to DNA catenation and causes camptothecin hypersensitivity. Through synthetic viability screening, we discovered that histone H4 K16 deacetylation drives the sensitivity of yeast cells to camptothecin and that inactivation of this pathway by mutating H4 K16 or the genes SIR1-4 suppresses much of the hypersensitivity of tof1∆ strains towards this agent...
April 7, 2017: EMBO Reports
https://www.readbyqxmd.com/read/28381790/the-wd40-domain-of-hira-is-essential-for-ri-nucleosome-assembly-in-xenopus-egg-extracts
#12
Ruibin Zhu, Mari Iwabuchi, Keita Ohsumi
Histone chaperones are a group of histone-binding proteins that facilitate the assembly of nucleosomes, the fundamental structural units of chromatin in eukaryotes. In nucleosome assembly, deposition of a histone H3-H4 tetramer onto DNA is the first and critical step, which is mediated by the histone chaperones HIRA and CAF-1. HIRA and CAF-1 are reportedly involved in DNA replication independent (RI) and replication coupled nucleosome assembly, respectively. However, the mechanisms by which they mediate histone deposition remain unclear...
2017: Cell Structure and Function
https://www.readbyqxmd.com/read/28380503/histone-deacetylase-inhibitor-m344-significantly-improves-nuclear-reprogramming-blastocyst-quality-and-in-vitro-developmental-capacity-of-cloned-pig-embryos
#13
L Jin, Q Guo, H-Y Zhu, X-X Xing, G-L Zhang, M-F Xuan, Q-R Luo, Z-B Luo, J-X Wang, H-M Choe, H-J Paek, X-J Yin, J-D Kang
M344 is a novel histone deacetylase inhibitor. There is no report on the effect of M344 treatment on the development of pig embryos after somatic cell nuclear transfer (SCNT). In the present study, we investigated the effect of M344 on the blastocyst formation rate in cloned embryos, acetylation level of histone H4 lysine 12 (AcH4K12), and the expression of pluripotency-related genes , , and . Our results indicated that treatment with 5 µ M344 for 6 h improved the development of porcine embryos, in comparison with the untreated group (25...
March 2017: Journal of Animal Science
https://www.readbyqxmd.com/read/28379497/the-elongation-factor-spt4-5-regulates-rna-polymerase-ii-transcription-through-the-nucleosome
#14
John B Crickard, Jaehyoun Lee, Tae-Hee Lee, Joseph C Reese
RNA polymerase II (RNAPII) passes through the nucleosome in a coordinated manner, generating several intermediate nucleosomal states as it breaks and then reforms histone-DNA contacts ahead of and behind it, respectively. Several studies have defined transcription-induced nucleosome intermediates using only RNA Polymerase. However, RNAPII is decorated with elongation factors as it transcribes the genome. One such factor, Spt4/5, becomes an integral component of the elongation complex, making direct contact with the 'jaws' of RNAPII and nucleic acids in the transcription scaffold...
April 3, 2017: Nucleic Acids Research
https://www.readbyqxmd.com/read/28379447/wdr5-in-porcine-preimplantation-embryos-expression-regulation-of-epigenetic-modifications-and-requirement-for-early-development1
#15
Biao Ding, Zubing Cao, Renyun Hong, Hui Li, Xiaoyuan Zuo, Lei Luo, Yunsheng Li, Weiping Huang, Wenyong Li, Kun Zhang, Yunhai Zhang
WDR5, a member of conserved WD40 protein family, is an essential component of the mixed lineage leukemia (MLL) complexes, which are crucial for numerous key biological processes including methylation of histone H3 lysine 4 (H3K4), self-renewal of embryonic stem cells, and formation of induced pluripotent stem cells. The expression pattern and functional role of WDR5 during porcine preimplantation embryonic development, however, remain unknown. Our results showed that the transcripts and protein of WDR5 exhibited stage-specific expression pattern in porcine early embryos...
March 31, 2017: Biology of Reproduction
https://www.readbyqxmd.com/read/28378802/histones-from-avian-erythrocytes-exhibit-antibiofilm-activity-against-methicillin-sensitive-and-methicillin-resistant-staphylococcus-aureus
#16
Megan Rose-Martel, Garima Kulshreshtha, Nahom Ahferom Berhane, Joelle Jodoin, Maxwell T Hincke
Staphylococcus aureus, a human pathogen associated with many illnesses and post-surgical infections, can resist treatment due to the emergence of antibiotic-resistant strains and through biofilm formation. The current treatments for chronic biofilm infections are antibiotics and/or surgical removal of the contaminated medical device. Due to higher morbidity and mortality rates associated with overuse/misuse of antibiotics, alternate treatments are essential. This study reports the antibiofilm activity of avian erythrocyte histones against methicillin-sensitive Staphylococcus aureus (MSSA) and methicillin-resistant Staphylococcus aureus (MRSA)...
April 5, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28375564/effects-of-histone-deacetylase-inhibitors-on-regenerative-cell-responses-in-human-dental-pulp-cells
#17
Z Luo, Z Wang, X He, N Liu, B Liu, L Sun, J Wang, F Ma, H Duncan, W He, P Cooper
AIM: To investigate the growth, migratory and adhesive effects of Trichostatin A (TSA) and Valproic acid (VPA), two HDACis, on hDPSCs. METHODOLOGY: To verify that TSA or VPA functions as an HDAC inhibitor, the expressions of histone H3 and H4 were examined using Western blotting analysis. hDPSC growth and metabolic activity was evaluated by MTT viability analysis at different time-points and by cell count experiments. The expression of cell cycle regulatory and the apoptosis associated proteins were examined by Western blot analysis...
April 4, 2017: International Endodontic Journal
https://www.readbyqxmd.com/read/28374988/crystal-structure-and-characterization-of-novel-human-histone-h3-variants-h3-6-h3-7-and-h3-8
#18
Hiroyuki Taguchi, Yan Xie, Naoki Horikoshi, Kazumitsu Maehara, Akihito Harada, Jumpei Nogami, Koichi Sato, Yasuhiro Arimura, Akihisa Osakabe, Tomoya Kujirai, Takeshi Iwasaki, Yuichiro Semba, Taro Tachibana, Hiroshi Kimura, Yasuyuki Ohkawa, Hitoshi Kurumizaka
Non-allelic histone variants are considered as epigenetic factors that regulate genomic DNA functions in eukaryotic chromosomes. In the present study, we identified three new human histone H3 variants (named H3.6, H3.7, and H3.8), which were previously annotated as pseudo-genes. H3.6 and H3.8 conserve the H3.3-specific amino acid residues, but H3.7 shares the specific amino acid residues with H3.1. We successfully reconstituted the nucleosome containing H3.6 in vitro, and determined its crystal structure. In the H3...
April 4, 2017: Biochemistry
https://www.readbyqxmd.com/read/28363874/label-free-protein-quantification-of-sodium-butyrate-treated-cho-cells-by-esi-uhr-tof-ms
#19
B Müller, C Heinrich, W Jabs, S Kaspar, A Schmidt, N Rodrigues de Carvalho, S P Albaum, C Baessmann, T Noll, R Hoffrogge
Effects of butyrate on CHO producer cells are contradictory, promoting productivity and at the same time repressing proliferation. Though in previous omics studies the background of butyrate impact on producer cells has been investigated, the knowledge about the mechanism is still very limited. As previous proteomic results on this field are mainly based on 2DE-gels, we conducted a label-free MS quantification, based on fast high resolution ESI-MS and a straight forward software solution, to gain insight in shifted cellular processes of CHO cells 25h after butyrate treatment...
March 28, 2017: Journal of Biotechnology
https://www.readbyqxmd.com/read/28360947/a-novel-method-for-isolation-of-histones-from-serum-and-its-implications-in-therapeutics-and-prognosis-of-solid-tumours
#20
Divya Reddy, Bharat Khade, Riddhi Pandya, Sanjay Gupta
BACKGROUND: Dysregulation in post-translational modifications of histones and their modifiers are now well-recognized as a hallmark of cancer and can be used as biomarkers and potential therapeutic targets for disease progression and prognosis. In most solid tumours, a biopsy is challenging, costly, painful or potentially risky for the patient. Therefore, non-invasive methods like 'liquid biopsy' for analysis of histone modifications and their modifiers if possible will be helpful in the better clinical management of cancer patients...
2017: Clinical Epigenetics
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