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Histone H4

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https://www.readbyqxmd.com/read/29321313/tip60-complex-inhibits-hbv-transcription
#1
Hironori Nishitsuji, Saneyuki Ujino, Keisuke Harada, Kunitada Shimotohno
Hepatitis B virus (HBV) is a global major health problem with over one million deaths annually caused by chronic liver damage. Understanding host factors that modulate HBV replication may aid the development of anti-HBV therapies. Our recent genome-wide small interfering RNA screen using recombinant HBV demonstrated that TIP60 inhibited HBV infection. Here, we show that TIP60 complex contributes to anti-HBV defense. The TIP60 complex bound to the HBV promoter and suppressed HBV transcription driven by the precore/core promoter...
January 10, 2018: Journal of Virology
https://www.readbyqxmd.com/read/29321206/revealing-the-protein-propionylation-activity-of-the-histone-acetyltransferase-males-absent-on-the-first-mof
#2
Zhen Han, Hong Wu, Sunjoo Kim, Xiangkun Yang, Qianjin Li, He Huang, Houjian Cai, Michael G Bartlett, Aiping Dong, Hong Zeng, Peter J Brown, Xiangjiao Yang, Cheryl H Arrowsmith, Yingming Zhao, Y George Zheng
Short-chain acylation of lysine residues recently emerges as a group of reversible posttranslational modifications in mammalian cells. The diversity of acylation further broadens the landscape and complexity of the proteome. Identification of regulatory enzymes and effector proteins for lysine acylation is critical to understand functions of these novel modifications at the molecular level. Here, we report that the MYST family of lysine acetyltransferases (KATs) possesses strong propionyltransferase activity both in vitro and in cellulo...
January 10, 2018: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/29304031/the-histone-deacetylase-inhibitor-valproic-acid-exerts-a-synergistic-cytotoxicity-with-the-dna-damaging-drug-ellipticine-in-neuroblastoma-cells
#3
Tereza Cerna, Jan Hrabeta, Tomas Eckschlager, Eva Frei, Heinz H Schmeiser, Volker M Arlt, Marie Stiborová
Neuroblastoma (NBL) originates from undifferentiated cells of the sympathetic nervous system. Chemotherapy is judged to be suitable for successful treatment of this disease. Here, the influence of histone deacetylase (HDAC) inhibitor valproate (VPA) combined with DNA-damaging chemotherapeutic, ellipticine, on UKF-NB-4 and SH-SY5Y neuroblastoma cells was investigated. Treatment of these cells with ellipticine in combination with VPA led to the synergism of their anticancer efficacy. The effect is more pronounced in the UKF-NB-4 cell line, the line with N-myc amplification, than in SH-SY5Y cells...
January 5, 2018: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29303701/increased-levels-of-serum-histone-h4-and-activated-protein-c-in-patients-with-active-rheumatoid-arthritis
#4
Wanchan Peng, Xianghui Liang, Yong Zhong, Yaou Zhou, Litao Hu, Wei Wang
OBJECTIVES: We aimed to examine the levels of serum H4 and activated protein C (APC) in rheumatoid arthritis (RA) and other autoimmune conditions, and investigate the associations between H4 or APC levels and disease activity indicators in RA. METHODS: Serum H4 and APC distribution was examined in samples from patients with RA, systemic lupus erythematosus (SLE), polymyositis (PM), and ankylosing spondylitis (AS), as well as in samples from healthy controls, using commercial ELISA kits...
December 27, 2017: Clinical and Experimental Rheumatology
https://www.readbyqxmd.com/read/29300974/the-elongation-factor-spn1-is-a-multi-functional-chromatin-binding-protein
#5
Sha Li, Adam R Almeida, Catherine A Radebaugh, Ling Zhang, Xu Chen, Liangqun Huang, Alison K Thurston, Anna A Kalashnikova, Jeffrey C Hansen, Karolin Luger, Laurie A Stargell
The process of transcriptional elongation by RNA polymerase II (RNAPII) in a chromatin context involves a large number of crucial factors. Spn1 is a highly conserved protein encoded by an essential gene and is known to interact with RNAPII and the histone chaperone Spt6. Spn1 negatively regulates the ability of Spt6 to interact with nucleosomes, but the chromatin binding properties of Spn1 are largely unknown. Here, we demonstrate that full length Spn1 (amino acids 1-410) binds DNA, histones H3-H4, mononucleosomes and nucleosomal arrays, and has weak nucleosome assembly activity...
December 30, 2017: Nucleic Acids Research
https://www.readbyqxmd.com/read/29299126/hdac-inhibition-as-a-treatment-concept-to-combat-temsirolimus-resistant-bladder-cancer-cells
#6
Eva Juengel, Ramin Najafi, Jochen Rutz, Sebastian Maxeiner, Jasmina Makarevic, Frederik Roos, Igor Tsaur, Axel Haferkamp, Roman A Blaheta
Introduction: Although the mechanistic target of rapamycin (mTOR) might be a promising molecular target to treat advanced bladder cancer, resistance develops under chronic exposure to an mTOR inhibitor (everolimus, temsirolimus). Based on earlier studies, we proposed that histone deacetylase (HDAC) blockade might circumvent resistance and investigated whether HDAC inhibition has an impact on growth of bladder cancer cells with acquired resistance towards temsirolimus. Results: The HDAC inhibitor valproic acid (VPA) significantly inhibited growth, proliferation and caused G0/G1 phase arrest in RT112res and UMUC-3res...
December 15, 2017: Oncotarget
https://www.readbyqxmd.com/read/29288791/chromatin-modifications-of-htert-gene-in-htert-immortalized-human-mesenchymal-stem-cells-upon-exposure-to-radiation
#7
Nedime Serakinci, Pınar Mega Tiber, Oya Orun
Regulation of telomerase activity is thought to participate in the cellular response to ionizing radiation. Epigenetic mechanisms play a role in this regulation, as well as other mechanisms such as transcription, phosphorylation, etc. Here, we investigated chromatin modifications in telomerase promoter upon exposure to ionizing radiation in human mesenchymal stem cells (hMSC) and telomerase-immortalized hMSCs (hMSC-telo1) together with a hMSC-telo1 cell line in which TRF2 expression was partially repressed (siTRF2 hMSC-telo1)...
December 27, 2017: European Journal of Medical Genetics
https://www.readbyqxmd.com/read/29274289/epigenetic-regulation-of-integrin-%C3%AE-6-transcription-induced-by-tgf-%C3%AE-1-in-human-oral-squamous-cell-carcinoma-cells
#8
Mingyan Xu, Liqin Yin, Yihuang Cai, Qingwei Hu, Jie Huang, Qing Ji, Yanping Hu, Wenxia Huang, Fan Liu, Songlin Shi, Xiaoling Deng
Overexpression of integrin αvβ6 is believed to play an important role in the invasion and metastasis of oral squamous cell carcinoma (OSCC). However, little is known about the molecular mechanisms leading to αvβ6 upregualtion in OSCC. As the integrin β6 (ITGB6) is the only partner with αv, the expression of αvβ6 is dependent on ITGB6, it is therefore pivotal to investigate the mechanisms underlying ITGB6 overexpression in OSCC. We previously reported the cloning and characterization of human ITGB6 gene...
December 23, 2017: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/29250163/mir-502-mediated-histone-methyltransferase-set8-expression-is-associated-with-clear-cell-renal-cell-carcinoma-risk
#9
Shenglei Zhang, Zhanjun Guo, Jinsheng Xu, Jing Wang, Junxia Zhang, Liwen Cui, Huiran Zhang, Yueping Liu, Yaling Bai
Genetic variants may affect the interactions between microRNAs (miRNAs/miRs) and their target genes by modulating their binding affinity or by creating, or destroying a miRNA-binding site. SET domain containing (lysine methyltransferase) 8 (SET8) is the sole lysine methyltransferase that catalyzes the monomethylation of histone H4 lysine 20, and is associated with tumor growth, invasion and metastasis. In the present study, the rs16917496 polymorphism within the miR-502 binding site of the SET8 mRNA 3' untranslated region (3'UTR) in patients with clear cell renal cell carcinoma (ccRCC) and healthy controls was genotyped...
December 2017: Oncology Letters
https://www.readbyqxmd.com/read/29233865/dicer-and-mmset-catalyzed-h4k20me2-recruits-the-nucleotide-excision-repair-factor-xpa-to-dna-damage-sites
#10
Shalaka Chitale, Holger Richly
Ultraviolet (UV) irradiation triggers the recruitment of DNA repair factors to the lesion sites and the deposition of histone marks as part of the DNA damage response. The major DNA repair pathway removing DNA lesions caused by exposure to UV light is nucleotide excision repair (NER). We have previously demonstrated that the endoribonuclease DICER facilitates chromatin decondensation during lesion recognition in the global-genomic branch of NER. Here, we report that DICER mediates the recruitment of the methyltransferase MMSET to the DNA damage site...
December 12, 2017: Journal of Cell Biology
https://www.readbyqxmd.com/read/29228701/a-systematic-assessment-of-chemical-genetic-and-epigenetic-factors-influencing-the-activity-of-anticancer-drug-kp1019-ffc14a
#11
Upendarrao Golla, Swati Swagatika, Sakshi Chauhan, Raghuvir Singh Tomar
KP1019 ([trans-RuCl4(1H-indazole)2]; FFC14A) is one of the promising ruthenium-based anticancer drugs undergoing clinical trials. Despite the pre-clinical and clinical success of KP1019, the mode of action and various factors capable of modulating its effects are largely unknown. Here, we used transcriptomics and genetic screening approaches in budding yeast model and deciphered various genetic targets and plethora of cellular pathways including cellular signaling, metal homeostasis, vacuolar transport, and lipid homeostasis that are primarily targeted by KP1019...
November 17, 2017: Oncotarget
https://www.readbyqxmd.com/read/29228602/fbxw8-dependent-degradation-of-mrfap1-in-anaphase-controls-mitotic-cell-death
#12
Duan-Zhuo Li, Shun-Fang Liu, Lan Zhu, Yu-Xing Wang, Yi-Xiang Chen, Jie Liu, Gang Hu, Xin Yu, Jian Li, Jin Zhang, Zhi-Xiang Wu, Han Lu, Wei Liu, Bin Liu
Mof4 family associated protein 1 (MRFAP1) is a 14 kDa nuclear protein, which involves in maintaining normal histone modification levels by negatively regulating recruitment of the NuA4 (nucleosome acetyltransferase of H4) histone acetyltransferase complex to chromatin. MRFAP1 has been identified as one of the most up-regulated proteins after NEDD8 (neural precursor cell expressed developmentally down-regulated 8) inhibition in multiple human cell lines. However, the biological function of MRFAP1 and the E3 ligase that targets MRFAP1 for destruction remain mysterious...
November 14, 2017: Oncotarget
https://www.readbyqxmd.com/read/29198113/characterization-of-chlamydomonas-reinhardtii-core-histones-by-top-down-mass-spectrometry-reveals-unique-algae-specific-variants-and-post-translational-modifications
#13
Aliyya Khan, Carlo K Eikani, Hana Khan, Anthony T Iavarone, James J Pesavento
The unicellular microalga Chlamydomonas reinhardtii has played an instrumental role in the development of many new fields (bio-products, biofuels, etc.) as well as the advancement of basic science (photosynthetic apparati, flagellar function, etc.). Chlamydomonas' versatility ultimately derives from the genes encoded in its genome and the way that the expression of these genes is regulated, which is largely influenced by a family of DNA binding proteins called histones. In this study, we characterize C. reinhardtii core histones, both variants and their post-translational modifications, by chromatographic separation followed by top-down mass spectrometry (TDMS)...
December 3, 2017: Journal of Proteome Research
https://www.readbyqxmd.com/read/29192002/ms-western-a-method-of-multiplexed-absolute-protein-quantification-is-a-practical-alternative-to-western-blotting
#14
Mukesh Kumar, Shai R Joseph, Martina Augsburg, Aliona Bogdanova, David Drechsel, Nadine L Vastenhouw, Frank Buchholz, Marc Gentzel, Andrej Shevchenko
Absolute quantification of proteins elucidates the molecular composition, regulation and dynamics of multiprotein assemblies and networks. Here we report on a method termed MS Western that accurately determines the molar abundance of dozens of user-selected proteins at the sub-femtomole level in whole cell or tissue lysates without metabolic or chemical labelling and without using specific antibodies. MS Western relies upon GeLC-MS/MS and quantifies proteins by in-gel co-digestion with an isotopically labelled QconCAT protein chimera composed of concatenated proteotypic peptides...
November 30, 2017: Molecular & Cellular Proteomics: MCP
https://www.readbyqxmd.com/read/29187567/linking-phytochrome-interacting-factor-to-histone-modification-in-plant-shade-avoidance
#15
Maolin Peng, Zepeng Li, Nana Zhou, Mengmeng Ma, Yupei Jiang, Aiwu Dong, Wen-Hui Shen, Lin Li
Shade avoidance syndrome (SAS) allows a plant grown in densely populated environment to maximize opportunities to access to sunlight. While it is well established that SAS is accompanied by gene expression changes, the underlying molecular mechanism needs to be elucidated. Here, we identify the H3K4me3/H3K36me3-binding proteins, Morf Related Gene (MRG) group proteins MRG1 and MRG2, as positive regulators of shade-induced hypocotyl elongation in Arabidopsis thaliana. MRG2 binds PHYTOCHROME- INTERACTING FACTOR 7 (PIF7) and regulates the expression of several common downstream target genes, including YUCCA8 and IAA19 involved in the auxin biosynthesis or response pathway and PRE1 involved in brassinosteroid regulation of cell elongation...
November 29, 2017: Plant Physiology
https://www.readbyqxmd.com/read/29186677/the-methyltransferase-setd8-is-essential-for-erythroblast-survival-and-maturation
#16
Jeffrey Malik, Jacquelyn A Lillis, Tyler Couch, Michael Getman, Laurie A Steiner
Erythropoiesis is a highly regulated process that generates enucleate red blood cells from committed erythroid progenitors. Chromatin condensation culminating in enucleation is a defining feature of this process. Setd8 is the sole enzyme that can mono-methylate histone H4, lysine 20 and is highly expressed in erythroblasts compared to most other cell types. Erythroid Setd8 deletion results in embryonic lethality from severe anemia due to impaired erythroblast survival and proliferation. Setd8 protein levels are also uniquely regulated in erythroblasts, suggesting a cell-type-specific role for Setd8 during terminal maturation...
November 28, 2017: Cell Reports
https://www.readbyqxmd.com/read/29186293/early-histone-h4-acetylation-during-chromatin-remodeling-in-equine-spermatogenesis
#17
Chelsea C Ketchum, Casey D Larsen, Alexis McNeil, Mirella L Meyer-Ficca, Ralph G Meyer
Chromatin remodeling during spermatogenesis culminates in the exchange of nucleosomes for transition proteins and protamines as an important part of spermatid development to give rise to healthy sperm. Comparative immunofluorescence analyses of equine and murine testis histological sections were used to characterize nucleoprotein exchange in the stallion. Histone H4 hyperacetylation is considered a key event of histone removal during the nucleoprotein transition to a protamine-based sperm chromatin structure...
November 25, 2017: Biology of Reproduction
https://www.readbyqxmd.com/read/29184082/identification-of-a-histone-family-gene-signature-for-predicting-the-prognosis-of-cervical-cancer-patients
#18
Xiaofang Li, Run Tian, Hugh Gao, Yongkang Yang, Bryan R G Williams, Michael P Gantier, Nigel A J McMillan, Dakang Xu, Yiqun Hu, Yan'e Gao
Heterogeneity in terms of tumor characteristics, prognosis, and survival among cancer patients is an unsolved issue. Here, we systematically analyzed the aberrant expression patterns of cervical cancer using RNA-Seq data from The Cancer Genome Atlas (TCGA). We incorporated gene profiling, molecular signatures, functional and pathway information with gene set enrichment and protein-protein interaction (PPI) network analysis, to identify sub-networks of genes. Those identified genes relating to DNA replication and DNA repair-mediated signaling pathways associated with systemic lupus erythematosus (SLE)...
November 28, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29180474/vitamin-c-sensitizes-melanoma-to-bet-inhibitors
#19
Gaofeng Wang, Sushmita Mustafi, Vladimir Camarena, Claude-Henry Volmar, Tyler C Huff, David W Sant, Shaun P Brothers, Zhao-Jun Liu, Claes Wahlestedt
Bromodomain and extra-terminal inhibitors (BETi) are promising cancer therapies, yet prominent side effects of BETi at effective doses have been reported in Phase I clinical trials. Here we screened a panel of small molecules targeting epigenetic modulators against human metastatic melanoma cells. Cells were pretreated with or without ascorbate (vitamin C), which promotes DNA demethylation and subsequently changes the sensitivity to drugs. Top hits were structurally unrelated BETi including JQ1, I-BET151, CPI-203, and BI-2536...
November 27, 2017: Cancer Research
https://www.readbyqxmd.com/read/29179736/phylogenetic-analysis-of-the-core-histone-doublet-and-dna-topo-ii-genes-of-marseilleviridae-evidence-of-proto-eukaryotic-provenance
#20
Albert J Erives
BACKGROUND: While the genomes of eukaryotes and Archaea both encode the histone-fold domain, only eukaryotes encode the core histone paralogs H2A, H2B, H3, and H4. With DNA, these core histones assemble into the nucleosomal octamer underlying eukaryotic chromatin. Importantly, core histones for H2A and H3 are maintained as neofunctionalized paralogs adapted for general bulk chromatin (canonical H2 and H3) or specialized chromatin (H2A.Z enriched at gene promoters and cenH3s enriched at centromeres)...
November 28, 2017: Epigenetics & Chromatin
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