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https://www.readbyqxmd.com/read/27928823/identification-of-differentially-expressed-genes-in-longissimus-muscle-of-pigs-with-high-and-low-intramuscular-fat-content-using-rna-sequencing
#1
K S Lim, K T Lee, J E Park, W H Chung, G W Jang, B H Choi, K C Hong, T H Kim
Intramuscular fat (IMF) content in pork is an important element of consumer preference and is positively correlated with meat quality, including tenderness and juiciness. With advances in RNA sequencing technologies, transcriptome-related differences can be associated with specific traits in animals. The objective of this study was to investigate differentially expressed genes (DEGs) closely related to IMF content in porcine longissimus muscle using RNA sequencing. A total of 107 Berkshire pigs were used for IMF content measurements, and significant differences between extremely high (H, n = 3) and low (L, n = 3) IMF content groups were found (P < 0...
December 7, 2016: Animal Genetics
https://www.readbyqxmd.com/read/27918556/effects-of-fatty-acid-synthase-inhibitors-on-lymphatic-vessels-an-in-vitro-and-in-vivo-study-in-a-melanoma-model
#2
Débora C Bastos, Jenny Paupert, Catherine Maillard, Fabiana Seguin, Marco A Carvalho, Michelle Agostini, Ricardo D Coletta, Agnès Noël, Edgard Graner
Fatty acid synthase (FASN) is responsible for the endogenous production of fatty acids from acetyl-CoA and malonyl-CoA. Its overexpression is associated with poor prognosis in human cancers including melanomas. Our group has previously shown that the inhibition of FASN with orlistat reduces spontaneous lymphatic metastasis in experimental B16-F10 melanomas, which is a consequence, at least in part, of the reduction of proliferation and induction of apoptosis. Here, we sought to investigate the effects of pharmacological FASN inhibition on lymphatic vessels by using cell culture and mouse models...
December 5, 2016: Laboratory Investigation; a Journal of Technical Methods and Pathology
https://www.readbyqxmd.com/read/27903831/dietary-mung-bean-protein-reduces-hepatic-steatosis-fibrosis-and-inflammation-in-male-mice-with-diet-induced-nonalcoholic-fatty-liver-disease
#3
Hitoshi Watanabe, Yuka Inaba, Kumi Kimura, Shun-Ichiro Asahara, Yoshiaki Kido, Michihiro Matsumoto, Takayasu Motoyama, Nobuhiko Tachibana, Shuichi Kaneko, Mitsutaka Kohno, Hiroshi Inoue
BACKGROUND: As the prevalence of nonalcoholic fatty liver disease (NAFLD), including steatosis and nonalcoholic steatohepatitis, is increasing, novel dietary approaches are required for the prevention and treatment of NAFLD. OBJECTIVE: We evaluated the potential of mung bean protein isolate (MuPI) to prevent NAFLD progression. METHODS: In Expts. 1 and 2, the hepatic triglyceride (TG) concentration was compared between 8-wk-old male mice fed a high-fat diet (61% of energy from fat) containing casein, MuPI, and soy protein isolate and an MuPI-constituent amino acid mixture as a source of amino acids (18% of energy) for 4 wk...
November 30, 2016: Journal of Nutrition
https://www.readbyqxmd.com/read/27884752/quantitative-proteomic-analysis-reveals-that-proteins-required-for-fatty-acid-metabolism-may-serve-as-diagnostic-markers-for-gastric-cancer
#4
Zhen Jiang, Hongchun Shen, Bo Tang, Qin Yu, Xingli Ji, Li Wang
BACKGROUND: Gastric cancer is one of the leading causes of cancer-related deaths worldwide. The sensitivities and specificities of current biomarkers for gastric cancer are insufficient for clinical detection, and new diagnostic tests are therefore urgently required. METHODS: A discovery set of gastric cancer and adjacent normal tissues were analyzed for differentially expressed proteins by labeling of peptide digests with isobaric tag for relative and absolute quantitation (iTRAQ) reagents followed by liquid chromatography-electrospray ionization-tandem mass spectrometry...
November 21, 2016: Clinica Chimica Acta; International Journal of Clinical Chemistry
https://www.readbyqxmd.com/read/27884183/rna-sequencing-for-profiling-goat-milk-transcriptome-in-colostrum-and-mature-milk
#5
Alessandra Crisà, Fabrizio Ferrè, Giovanni Chillemi, Bianca Moioli
BACKGROUND: In this work we aimed at sequencing and assembling the goat milk transcriptome corresponding at colostrum and 120 days of lactation. To reconstruct transcripts we used both the genome as reference, and a de novo assembly approach. Additionally, we aimed at identifying the differentially expressed genes (DEGs) between the two lactation stages and at analyzing the expression of genes involved in oligosaccharides metabolism. RESULTS: A total of 44,635 different transcripts, organized in 33,757 tentative genes, were obtained using the goat genome as reference...
November 25, 2016: BMC Veterinary Research
https://www.readbyqxmd.com/read/27879277/eukaryotic-translation-initiation-factor-5a2-promotes-metabolic-reprogramming-in-hepatocellular-carcinoma-cells
#6
Ting-Ting Cao, Shu-Hai Lin, Li Fu, Zhi Tang, Chi-Ming Che, Li-Yi Zhang, Xiao-Yan Ming, Teng-Fei Liu, Xu-Ming Tang, Bin-Bin Tan, Di Xiang, Feng Li, On-Yee Chan, Dan Xie, Zongwei Cai, Xin-Yuan Guan
Reprogramming of intracellular metabolism is common in liver cancer cells. Understanding the mechanisms of cell metabolic reprogramming may present a new basis for liver cancer treatment. In our previous study we reported that a novel oncogene eukaryotic translation initiation factor 5A2 (EIF5A2) promotes tumorigenesis under hypoxic condition. Here, we aim to investigate the role of EIF5A2 in cell metabolic reprogramming during hepatocellular carcinoma (HCC) development. In this study, we reported that the mRNA level of EIF5A2 was up-regulated in 59/105 (56...
November 22, 2016: Carcinogenesis
https://www.readbyqxmd.com/read/27878829/genetic-association-of-plag1-scd-cyp7b1-and-fasn-snps-and-their-effects-on-carcass-weight-intramuscular-fat-and-fatty-acid-composition-in-hanwoo-steers-korean-cattle
#7
Hyun Joo Kim, Aditi Sharma, Soo Hyun Lee, Doo Ho Lee, Da Jeong Lim, Yong Min Cho, Boh Suk Yang, Seung Hwan Lee
No abstract text is available yet for this article.
November 22, 2016: Animal Genetics
https://www.readbyqxmd.com/read/27873461/human-peripheral-blood-mononuclear-cell-in-vitro-system-to-test-the-efficacy-of-food-bioactive-compounds-effects-of-polyunsaturated-fatty-acids-and-their-relation-with-bmi
#8
Margalida Cifre, Rubén Díaz-Rúa, Rubén Varela-Calviño, Bàrbara Reynés, Jordi Pericás-Beltrán, Andreu Palou, Paula Oliver
SCOPE: To analyse usefulness of isolated human peripheral blood mononuclear cells (PBMC) to rapidly/easily reflect n-3 long-chain polyunsaturated fatty acid (LCPUFA) effects on lipid metabolism/inflammation gene profile, and evaluate if these effects are BMI-dependent. METHODS AND RESULTS: PBMC from normoweight (NW) and overweight/obese (OW/OB) subjects were incubated with physiological doses of docosahexaenoic (DHA), eicosapentaenoic acid (EPA), or their combination...
November 22, 2016: Molecular Nutrition & Food Research
https://www.readbyqxmd.com/read/27869741/isoliquiritigenin-attenuates-atherogenesis-in-apolipoprotein-e-deficient-mice
#9
Fen Du, Quzhen Gesang, Jia Cao, Mei Qian, Li Ma, Dongfang Wu, Hong Yu
Isoliquiritigenin (ISL) exhibits antioxidation and anti-inflammation activity. We sought to investigate the effects and mechanism of ISL on the development of atherosclerotic lesions in apolipoprotein E-deficient (apoE(-/-)) mice. Firstly, we determined that ISL reduced the mRNA levels of inflammatory factors interleukin 6 (IL-6), tumor necrosis factor α (TNF-α), and monocyte chemotactic protein-1 (MCP-1), while it increased the expression of several lipoprotein-related genes in peritoneal macrophages treated with lipopolysaccharide (LPS)...
November 18, 2016: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/27868245/the-influence-of-sex-and-gonadectomy-on-hepatic-and-brain-fatty-acid-composition-lipogenesis-and-%C3%AE-oxidation
#10
K Starčević, N Filipović, M Šperanda, M Đidara, T Mašek
The aim of this study was to investigate the influence of sex and castration of rats on liver and brain fatty acid profile and liver mRNA expression of genes involved in lipogenesis and β-oxidation. Castration significantly increased body weight and liver index and decreased serum triglyceride content in the female rats. The fatty acid composition of the liver tissue was influenced by sex and castration. Male rats had higher content of C16:0, C18:1n7, C18:2n6 and C22:5n3, while female rats had higher content of C18:0, C20:4n6 and C22:6n3...
November 21, 2016: Journal of Animal Physiology and Animal Nutrition
https://www.readbyqxmd.com/read/27865496/peroxisome-proliferator-activated-receptor-delta-facilitates-lipid-secretion-and-catabolism-of-fatty-acids-in-dairy-goat-mammary-epithelial-cells
#11
H B Shi, C H Zhang, W Zhao, J Luo, J J Loor
In rodents, peroxisome proliferator-activated receptor delta (PPARD) is associated primarily with catabolism of fatty acids. However, the role of PPARD in regulating lipid metabolism in ruminant mammary gland remains unknown. In the present study, we assessed the mRNA abundance of PPARD at 3 stages of lactation in goat mammary tissue. Results revealed that PPARD had lower expression at peak lactation than in the nonlactating period. Luciferase assays revealed that GW0742 (GW), a specific PPARD ligand, enhanced the activity of the PPARD response element in goat mammary epithelial cells...
November 16, 2016: Journal of Dairy Science
https://www.readbyqxmd.com/read/27832864/impaired-adipose-tissue-lipid-storage-but-not-altered-lipolysis-contributes-to-elevated-levels-of-nefa-in-type-2-diabetes-degree-of-hyperglycemia-and-adiposity-are-important-factors
#12
Maria J Pereira, Stanko Skrtic, Petros Katsogiannos, Niclas Abrahamsson, Cherno O Sidibeh, Santosh Dahgam, Marianne Månsson, Ulf Risérus, Joel Kullberg, Jan W Eriksson
BACKGROUND: Elevated levels of circulating non-esterified fatty acids (NEFA) mediate many adverse metabolic effects. In this work we aim to determine the impact of type 2 diabetes (T2D), glycemic control and obesity on lipolysis regulation. DESIGN AND PARTICIPANTS: 20 control and 20 metformin-treated T2D subjects were matched for sex (10M/10 F), age (58±11 vs 58±9 y) and BMI (30.8±4.6 vs 30.7±4.9kg/m(2)). In vivo lipolysis was assessed during a 3h-OGTT with plasma glycerol and NEFA levels...
December 2016: Metabolism: Clinical and Experimental
https://www.readbyqxmd.com/read/27827939/effects-of-di-2-ethylhexyl-phthalate-on-lipid-metabolism-by-the-jak-stat-pathway-in-rats
#13
Yiyang Jia, Te Liu, Liting Zhou, Jian Zhu, Juan Wu, Di Sun, Jin Xu, Qi Wang, Huaiji Chen, Feng Xu, Yuezhu Zhang, Tianrong Zhang, Hongbo Liu, Lin Ye
The most widely used plasticizer, di-(2-ethylhexyl) phthalate (DEHP), is known to affect lipid metabolism and adipogenesis. We studied the effects of dietary DEHP exposure on metabolism in rats as well as the role of the JAK/STAT pathway in this process. Eighty rats were exposed to DEHP (0, 5, 50 and 500 mg/kg/d) through dietary intake for 4 weeks. We then collected blood samples, liver, and adipose tissues to detect modifications in the levels of serum lipids, leptin, adiponectin and insulin. JAK3, STAT5a and PPARγ expression were detected at both the gene and protein levels...
November 4, 2016: International Journal of Environmental Research and Public Health
https://www.readbyqxmd.com/read/27814245/effects-of-periconception-cadmium-and-mercury-co-administration-to-mice-on-indices-of-chronic-disease-in-male-offspring-at-maturity
#14
Cagri Camsari, Joseph K Folger, Devin McGee, Steven J Bursian, Hongbing Wang, Jason G Knott, George W Smith
BACKGROUND: Long-term exposure to the heavy metals cadmium (Cd) and mercury (Hg) is known to increase risk of chronic diseases. However, to our knowledge, exposure to Cd and Hg beginning at the periconception period has not been studied to date. OBJECTIVE: The effect of co-administration of Cd and Hg during early development on indices of chronic diseases at adulthood was examined. METHODS: Adult female CD1 mice were subcutaneously administered a combination of cadmium chloride (CdCl2) and methylmercury (II) chloride (CH3HgCl; 0, 0...
November 4, 2016: Environmental Health Perspectives
https://www.readbyqxmd.com/read/27813492/long-non-coding-rna-pvt1-promotes-osteosarcoma-development-by-acting-as-a-molecular-sponge-to-regulate-mir-195
#15
Quan Zhou, Fengli Chen, Jiali Zhao, Baojun Li, Yong Liang, Wei Pan, Shaoxian Zhang, Xinhong Wang, Donghui Zheng
A growing body of evidence has indicated that long non-coding RNAs (lncRNAs) serve as competing endogenous RNAs (ceRNAs) during oncogenesis. In this study, the qRT-PCR results indicated that the lncRNA PVT1 is overexpressed in osteosarcoma and decreased the survival rate of osteosarcoma patients. MTT and clonal colony formation assays were used to detect the effect of PVT1 on proliferation, and flow cytometry was performed to assess apoptosis and the cell cycle. A Transwell assay was used to analyze migration and invasion...
November 2, 2016: Oncotarget
https://www.readbyqxmd.com/read/27806377/fatty-acid-synthesis-configures-the-plasma-membrane-for-inflammation-in-diabetes
#16
Xiaochao Wei, Haowei Song, Li Yin, Michael G Rizzo, Rohini Sidhu, Douglas F Covey, Daniel S Ory, Clay F Semenkovich
Dietary fat promotes pathological insulin resistance through chronic inflammation. The inactivation of inflammatory proteins produced by macrophages improves diet-induced diabetes, but how nutrient-dense diets induce diabetes is unknown. Membrane lipids affect the innate immune response, which requires domains that influence high-fat-diet-induced chronic inflammation and alter cell function based on phospholipid composition. Endogenous fatty acid synthesis, mediated by fatty acid synthase (FAS), affects membrane composition...
November 2, 2016: Nature
https://www.readbyqxmd.com/read/27800008/metabolic-gene-expression-profile-in-circulating-mononuclear-cells-reflects-obesity-associated-metabolic-inflexibility
#17
Sonia Baig, Ehsan Parvaresh Rizi, Muhammad Shabeer, Vanna Chhay, Shao Feng Mok, Tze Ping Loh, Faidon Magkos, Antonio Vidal-Puig, E Shyong Tai, Chin Meng Khoo, Sue-Anne Toh
BACKGROUND: Obesity is associated with an impaired ability to switch from fatty acid to glucose oxidation during the fasted to fed transition, particularly in skeletal muscle. However, whether such metabolic inflexibility is reflected at the gene transcription level in circulatory mononuclear cells (MNC) is not known. METHODS: The whole-body respiratory quotient (RQ) and transcriptional regulation of genes involved in carbohydrate and lipid metabolism in MNC were measured during fasting and in response (up to 6 h) to high-carbohydrate and high-fat meals in nine lean insulin-sensitive and nine obese insulin-resistant men...
2016: Nutrition & Metabolism
https://www.readbyqxmd.com/read/27791122/fasn-regulates-cellular-response-to-genotoxic-treatments-by-increasing-parp-1-expression-and-dna-repair-activity-via-nf-%C3%AE%C2%BAb-and-sp1
#18
Xi Wu, Zizheng Dong, Chao J Wang, Lincoln James Barlow, Valerie Fako, Moises A Serrano, Yue Zou, Jing-Yuan Liu, Jian-Ting Zhang
Fatty acid synthase (FASN), the sole cytosolic mammalian enzyme for de novo lipid synthesis, is crucial for cancer cell survival and associates with poor prognosis. FASN overexpression has been found to cause resistance to genotoxic insults. Here we tested the hypothesis that FASN regulates DNA repair to facilitate survival against genotoxic insults and found that FASN suppresses NF-κB but increases specificity protein 1 (SP1) expression. NF-κB and SP1 bind to a composite element in the poly(ADP-ribose) polymerase 1 (PARP-1) promoter in a mutually exclusive manner and regulate PARP-1 expression...
October 24, 2016: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/27771090/effects-of-nutrient-intake-level-on-mammary-parenchyma-growth-and-gene-expression-in-crossbred-holstein-%C3%A3-gyr-prepubertal-heifers
#19
M M D C A Weller, Ronan L Albino, M I Marcondes, W Silva, K M Daniels, M M Campos, M S Duarte, M L Mescouto, F F Silva, S E F Guimarães
This study investigated the effects of increased nutrient intake levels on prepubertal mammary parenchyma development in crossbreed (Holstein × Gyr) dairy heifers. Eighteen heifers age 3 to 4 mo were fed 1 of 3 nutrient intake levels (n = 6 per treatment) designed to sustain an average daily gain of 0.0 kg/d (maintenance, MA), 0.5 kg/d (low gain, LG), or 1.0 kg/d (high gain, HG). Serum blood samples collected on d 42 and 84 after a 12-h fast were analyzed for triglycerides, leptin, insulin, and insulin-like growth factor 1 (IGF-1)...
October 19, 2016: Journal of Dairy Science
https://www.readbyqxmd.com/read/27769711/esculetin-prevents-non-alcoholic-fatty-liver-in-diabetic-mice-fed-high-fat-diet
#20
Ra-Yeong Choi, Ju Ri Ham, Mi-Kyung Lee
This study investigated the effects and mechanism of esculetin (6,7-dihydroxycoumarin) on non-alcoholic fatty liver in diabetic mice fed high-fat diet (HFD). The diabetic mice model was induced by injection of streptozotocin, after which they were fed HFD diet with or without esculetin for 11 weeks. Non-diabetic mice were provided a normal diet. Diabetes induced hepatic hypertrophy, lipid accumulation and droplets; however, esculetin reversed these changes. Esculetin treatment in diabetic mice fed HFD significantly down-regulated expression of lipid synthesis genes (Fasn, Dgat2 and Plpp2) and inflammation genes (Tlr4, Myd88, Nfkb, Tnfα and Il6)...
December 25, 2016: Chemico-biological Interactions
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