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https://www.readbyqxmd.com/read/28811490/mammary-tumors-induce-central-pro-inflammatory-cytokine-expression-but-not-behavioral-deficits-in-balb-c-mice
#1
William H Walker Ii, Jeremy C Borniger, Surbhi, Abigail A Zalenski, Stevie L Muscarella, Julie A Fitzgerald, Ning Zhang, Monica M Gaudier-Diaz, A Courtney DeVries
Breast cancer survivors are more likely to develop mood disorders and cognitive deficits than women in the general population. Previous studies suggest that peripheral tumors elicit central pro-inflammatory cytokine production, in turn leading to depression and cognitive deficits. In the current study, two cohorts of female Balb/C mice received bilateral orthotopic injections of syngeneic 67NR, 4T07, or 4T1cells (1 × 10(5) cells per injection) to induce mammary tumors. Approximately three weeks later, learned fear (via fear conditioning) or depressive-like behavior (via tail suspension and forced swim test) was assessed...
August 15, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28811469/rgd-delivery-of-truncated-coagulase-to-tumor-vasculature-affords-local-thrombotic-activity-to-induce-infarction-of-tumors-in-mice
#2
Rana Jahanban-Esfahlan, Khaled Seidi, Hassan Monhemi, Amir Daei Farshchi Adli, Babak Minofar, Peyman Zare, Davoud Farajzadeh, Safar Farajnia, Ramezan Behzadi, Mehran Mesgari Abbasi, Nosratollah Zarghami, Tahereh Javaheri
Induction of thrombosis in tumor vasculature represents an appealing strategy for combating cancer. Herein, we combined unique intrinsic coagulation properties of staphylocoagulase with new acquired functional potentials introduced by genetic engineering, to generate a novel bi-functional fusion protein consisting of truncated coagulase (tCoa) bearing an RGD motif on its C-terminus for cancer therapy. We demonstrated that free coagulase failed to elicit any significant thrombotic activity. Conversely, RGD delivery of coagulase retained coagulase activity and afforded favorable interaction of fusion proteins with prothrombin and αvβ3 endothelial cell receptors, as verified by in silico, in vitro, and in vivo experiments...
August 15, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28799743/reducing-interstitial-fluid-pressure-ifp-and-inhibiting-pulmonary-metastasis-of-breast-cancer-by-gelatin-modified-cationic-lipid-nanoparticles
#3
Xuan Gao, Jun Zhang, Zun Huang, Tiantian Zuo, Qing Lu, Guangyu Wu, Qi Shen
Interstitial fluid pressure (IFP) in tumor is much higher than that in normal tissue and it constitutes a great obstacle for the delivery of anti-tumor drugs, which makes it a potential target for cancer therapy. In this study, cationic nanostructured lipid carriers (NLCs) were modified by gelatin with low molecular weight in order to achieve the desirable reduction of tumor IFP and improve the drug delivery as well as the chemotherapy on tumor proliferation and pulmonary metastasis. The nanoparticles were used to entrap three drugs - docetaxel (DTX), quercetin (Qu) and imatinib (IMA) - with high encapsulation efficiency of 89...
August 11, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28798322/luciferase-expression-allows-bioluminescence-imaging-but-imposes-limitations-on-the-orthotopic-mouse-4t1-model-of-breast-cancer
#4
V P Baklaushev, A Kilpeläinen, S Petkov, M A Abakumov, N F Grinenko, G M Yusubalieva, A A Latanova, I L Gubskiy, F G Zabozlaev, E S Starodubova, T O Abakumova, M G Isaguliants, V P Chekhonin
Implantation of reporter-labeled tumor cells in an immunocompetent host involves a risk of their immune elimination. We have studied this effect in a mouse model of breast cancer after the orthotopic implantation of mammary gland adenocarcinoma 4T1 cells genetically labelled with luciferase (Luc). Mice were implanted with 4T1 cells and two derivative Luc-expressing clones 4T1luc2 and 4T1luc2D6 exhibiting equal in vitro growth rates. In vivo, the daughter 4T1luc2 clone exhibited nearly the same, and 4T1luc2D6, a lower growth rate than the parental cells...
August 10, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28791524/histological-analysis-of-anti-cancer-drug-loaded-targeted-mn-zns-quantum-dots-in-metastatic-lesions-of-4t1-challenged-mice
#5
Ibrahim Birma Bwatanglang, Faruq Mohammad, Nor Azah Yusof, Nurul Elyani Mohammed, Nadiah Abu, Noorjahan Banu Alitheen, Jaafar Abdullah, Mohd Zubir Hussein, Yusuf Abba, Noraini Nordin, Nur Rizi Zamberi
5-Fluororaucil (5-FU) as anti-cancer drug was reported to induce thymidine synthase (TS) overexpression and cancer cell resistance. To improve its therapeutic efficacy and selective targeting, here we developed a targeted delivery system mediated by the active ligand-folate receptor chemistry to deliver the 5-FU drug selectively into the tumor microenvironment. The preparation was achieved by exploring chitosan (CS)-biopolymer based system with folic acid (FA)-conjugation. The 5-FU@FACS-Mn:ZnS quantum dots (QDs) based on the histological assessment conducted in the 4T1 challenged mice showed an improved tumor remission in the liver, spleen and lungs...
August 8, 2017: Journal of Materials Science. Materials in Medicine
https://www.readbyqxmd.com/read/28790175/antibody-mediated-blockade-of-jmjd6-interaction-with-collagen-i-exerts-antifibrotic-and-antimetastatic-activities
#6
Silvia Miotti, Alessandro Gulino, Renata Ferri, Mariella Parenza, Agnieszka Chronowska, Daniele Lecis, Sabina Sangaletti, Elda Tagliabue, Claudio Tripodo, Mario P Colombo
JMJD6 is known to localize in the nucleus exerting histone arginine demethylase and lysyl hydroxylase activities. A novel localization of JMJD6 in the extracellular matrix, resulting from its secretion as a soluble protein, was unveiled by a new anti-JMJD6 mAb called P4E11 which was developed to identify new targets in the stroma. Recombinant JMJD6 binds with collagen type I (Coll-I), and distinct JMJD6 peptides interfere with collagen fibrillogenesis, collagen-fibronectin interaction, and adhesion of human tumor cells to the collagen substrate...
August 8, 2017: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
https://www.readbyqxmd.com/read/28789518/theranostic-iron-oxide-gold-ion-nanoprobes-for-mr-imaging-and-non-invasive-rf-hyperthermia
#7
Sajid Fazal, Bindhu Paul-Prasanth, Shantikumar Nair, Deepthy Menon
This work focuses on the development of a nanoparticulate system that can be used for MR imaging and E-field non-invasive radiofrequency (RF) hyperthermia. For this purpose, an amine-functional gold ion complex, (GIC) [Au(III)(diethylenetriamine)Cl]Cl2 which generates heat upon RF exposure, was conjugated to carboxyl functional polyacrylic acid capped iron-oxide nanoparticles (IO-PAA NPs) to form IO-GIC NPs of size ~100 nm. The multimodal superparamagnetic IO-GIC NPs produced T2-contrast on MR imaging and unlike IO-PAA NPs, generated heat on RF exposure...
August 9, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28775257/sers-investigation-of-cancer-cells-treated-with-pdt-quantification-of-cell-survival-and-follow-up
#8
A B Veloso, J P F Longo, L A Muehlmann, B F Tollstadius, P E N Souza, R B Azevedo, P C Morais, S W da Silva
In this study Surface Enhanced Raman Spectroscopy (SERS) data recorded from mouse mammary glands cancer cells (4T1 cell line) was used to assess information regarding differences between control, death and viable cells after Photodynamic Therapy (PDT) treatment. The treatment used nanoemulsions (NE/PS) loaded with different chloroaluminumphthalocyanine (ClAlP) photosensitizer (PS) contents (5 and 10 µmol × L(-1)) and illumination (660 nm wavelength) at 10 J × cm(-2) (10 minutes). The SERS data revealed significant molecular alterations in proteins and lipids due to the PDT treatment...
August 3, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28773010/synthesis-of-au-np-mos%C3%A2-quantum-dots-core-shell-nanocomposites-for-sers-bio-analysis-and-label-free-bio-imaging
#9
Xixi Fei, Zhiming Liu, Yuqing Hou, Yi Li, Guangcun Yang, Chengkang Su, Zhen Wang, Huiqing Zhong, Zhengfei Zhuang, Zhouyi Guo
In this work, we report a facile method using MoS₂ quantum dots (QDs) as reducers to directly react with HAuCl₄ for the synthesis of Au nanoparticle@MoS₂ quantum dots (Au NP@MoS₂ QDs) core@shell nanocomposites with an ultrathin shell of ca. 1 nm. The prepared Au NP@MoS₂ QDs reveal high surface enhanced Raman scattering (SERS) performance regarding sensitivity as well as the satisfactory SERS reproducibility and stability. The limit of detection of the hybrids for crystal violet can reach 0.5 nM with a reasonable linear response range from 0...
June 13, 2017: Materials
https://www.readbyqxmd.com/read/28772282/non-conventional-role-of-haemoglobin-beta-in-breast-malignancy
#10
Marco Ponzetti, Mattia Capulli, Adriano Angelucci, Luca Ventura, Simona Delle Monache, Cinzia Mercurio, Alessia Calgani, Patrizia Sanità, Anna Teti, Nadia Rucci
BACKGROUND: Besides its role as oxygen transporter, recent findings suggest that haemoglobin beta (HBB) may have roles in other contexts. METHODS: We evaluated the impact of HBB expression in primary human breast cancers, and in breast cancer cell lines overexpressing HBB by in vitro and in vivo studies. Publicly available microarray databases were used to perform multivariate survival analyses. RESULTS: A significantly higher expression of HBB was observed in invasive carcinoma histotypes vs in situ counterparts, along with a positive correlation between HBB and the Ki67 proliferation marker...
August 3, 2017: British Journal of Cancer
https://www.readbyqxmd.com/read/28765708/folate-modified-annonaceous-acetogenins-nanosuspensions-and-their-improved-antitumor-efficacy
#11
Jingyi Hong, Zhonghao Sun, Yijing Li, Yifei Guo, Yonghong Liao, Meifeng Liu, Xiangtao Wang
Annonaceous acetogenins (ACGs) are a large family of fatty acid derived natural products that are exclusively isolated from the Annonaceae species. Many members of this diverse family have a broad spectrum of biological activities, the most impressive of which is anticancer activity. However, their poor solubility and severe toxicity restrict their clinical application, and their complicated composition hinders their formulation and drug delivery. In this study, β-cyclodextrin was modified with folic acid (FA) and then combined with soybean lecithin to prepare FA-modified ACGs nanosuspensions (FA-ACGs-NSps)...
2017: International Journal of Nanomedicine
https://www.readbyqxmd.com/read/28758755/inflammatory-monocytes-loading-protease-sensitive-nanoparticles-enable-lung-metastasis-targeting-and-intelligent-drug-release-for-anti-metastasis-therapy
#12
Xinyu He, Haiqiang Cao, Hong Wang, Tao Tan, Haijun Yu, Pengcheng Zhang, Qi Yin, Zhiwen Zhang, Yaping Li
Metastasis causes high mortality of breast cancer, and the inability of drug delivery to metastatic sites remains a crucial challenge for antimetastasis therapy. Herein, we report that inflammatory monocytes loading legumain-activated nanoparticles can actively target lung metastases and initiate metastasis-specific intelligent drug release for antimetastasis therapy. The cytotoxic mertansine is conjugated to poly(styrene-co-maleic anhydride) with a legumain-sensitive peptide and self-assembled into nanoparticles (SMNs), and then loaded into inflammatory monocytes to prepare the SMNs-loaded monocytes delivery system (M-SMNs)...
August 2, 2017: Nano Letters
https://www.readbyqxmd.com/read/28756536/therapeutic-blockade-of-foxp3-in-experimental-breast-cancer-models
#13
Mariela A Moreno Ayala, María Florencia Gottardo, Mercedes Imsen, Antonela S Asad, Elisa Bal de Kier Joffé, Noelia Casares, Juan José Lasarte, Adriana Seilicovich, Marianela Candolfi
PURPOSE: Regulatory T cells (Tregs) impair the clinical benefit of cancer immunotherapy. To optimize the antitumor efficacy of therapeutic dendritic cell (DC) vaccines, we aimed to inhibit Foxp3, a transcription factor required for Treg function. METHODS: Mice bearing established syngeneic LM3 and 4T1 breast tumors were treated with antitumor DC vaccines and a synthetic peptide (P60) that has been shown to inhibit Foxp3. RESULTS: Treatment with P60 improved the therapeutic efficacy of DC vaccines in these experimental models...
July 29, 2017: Breast Cancer Research and Treatment
https://www.readbyqxmd.com/read/28754875/cbp501-suppresses-macrophage-induced-cancer-stem-cell-like-features-and-metastases
#14
Naoki Mine, Sayaka Yamamoto, Naoya Saito, Takuji Sato, Keiichi Sakakibara, Donald W Kufe, Daniel D VonHoff, Takumi Kawabe
CBP501 is an anti-cancer drug candidate which has been shown to increase cis-diamminedichloro-platinum (II) (CDDP) uptake into cancer cell through calmodulin (CaM) inhibition. However, the effects of CBP501 on the cells in the tumor microenvironment have not been addressed. Here, we investigated new aspects of the potential anti-tumor mechanism of action of CBP501 by examining its effects on the macrophages.Macrophages contribute to cancer-related inflammation and sequential production of cytokines such as IL-6 and TNF-α which cause various biological processes that promote tumor initiation, growth and metastasis (1)...
July 17, 2017: Oncotarget
https://www.readbyqxmd.com/read/28751740/2-dodecyl-6-methoxycyclohexa-2-5-diene-1-4-dione-inhibits-the-growth-and-metastasis-of-breast-carcinoma-in-mice
#15
Chunxia Chen, Zhihuan Nong, Qiuqiao Xie, Junhui He, Wene Cai, Xiuneng Tang, Xiaoyu Chen, Renbin Huang, Ying Gao
Metastasis causes approximately 90% of breast cancer-related deaths in women. Previously, we have demonstrated that 2-dodecyl-6-methoxycyclohexa-2,5-diene- 1,4-dione (DMDD) remarkably inhibited the growth of human breast cancer cells with little toxicity. In this study, we investigated the toxicity and efficacy of DMDD to treat metastatic breast tumors using an in vivo mouse model of the 4T1 mammary carcinoma. DMDD caused no observable toxicity and significantly extended the survival of 4T1 tumor-bearing mice...
July 27, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28750038/low-calcium-diet-increases-4t1-mammary-tumor-carcinoma-cell-burden-and-bone-pathology-in-mice
#16
Wendan Wang, Jody L Gordon, Kenneth A Philbrick, Xujuan Yang, Adam J Branscum, Christiane V Löhr, Wanda M Haschek, Russell T Turner, Urszula T Iwaniec, William G Helferich
Breast cancer metastasizes to bone in the majority of patients with advanced disease. We investigated the effects of inadequate dietary calcium (Ca) on bone turnover, tumor growth, and bone response to tumor in tibia inoculated with 4T1 mammary carcinoma cells. Nine-month-old female Balb/c mice were placed on an adequate Ca (5 g/kg diet, n = 30) or low Ca (80 mg/kg diet, n = 31) diet for 14 days, then injected intratibially with 1,000 4T1 cells (transfected with luciferase for bioluminescence imaging), and sacrificed at 5, 10, or 21 days post-inoculation (n = 7-10 mice/group)...
2017: PloS One
https://www.readbyqxmd.com/read/28747336/an-innovative-intermittent-hypoxia-model-in-cell-cultures-allowing-fast-po2-oscillations-with-minimal-gas-consumption
#17
Mélanie Minoves, Jessica Morand, Frédéric Perriot, Morgane Chatard, Brigitte Gonthier, Emeline Lemarié, Jean-Baptiste Menut, Jan Polak, Jean-Louis Pepin, Diane Godin-Ribuot, Anne Briancon-Marjollet
Performing hypoxia-reoxygenation cycles in cell culture with a cycle duration accurately reflecting what occurs in obstructive sleep apnea (OSA) patients is a difficult but crucial technical challenge. Our goal was to develop a novel device to expose multiple cell culture dishes to intermittent hypoxia (IH) cycles relevant to OSA with limited gas consumption. With gas flows as low as 200 mL/min, our combination of plate holders with gas-permeable cultureware generates rapid normoxia-hypoxia cycles. Cycles alternating 1 minute at 20% O2 followed by 1 minute at 2% O2 result in pO2 values ranging from 124 to 44mmHg...
July 26, 2017: American Journal of Physiology. Cell Physiology
https://www.readbyqxmd.com/read/28744322/visualization-of-tumor-immune-interaction-target-specific-imaging-of-s100a8-a9-reveals-pre-metastatic-niche-establishment
#18
Michel Eisenblaetter, Fabian Flores-Borja, Jae Jin Lee, Christina Wefers, Hannah Smith, Rebekka Hueting, Margaret S Cooper, Philip J Blower, Dominic Patel, Manuel Rodriguez-Justo, Hanna Milewicz, Thomas Vogl, Johannes Roth, Andrew Tutt, Tobias Schaeffter, Tony Ng
Background Systemic cancer spread is preceded by the establishment of a permissive microenvironment in the target tissue of metastasis - the premetastatic niche. As crucial players in establishment of the pre-metastatic niche, myeloid derived suppressor cells (MDSC) release S100A8/A9, an exosomal protein that contributes to metastasis, angiogenesis, and immune suppression. We report the application of antibody-based single-photon emission computed tomography (SPECT) for detection of S100A8/A9 in vivo as an imaging marker for pre-metastatic tissue priming...
2017: Theranostics
https://www.readbyqxmd.com/read/28743887/tumor-derived-exosomes-induce-the-formation-of-neutrophil-extracellular-traps-implications-for-the-establishment-of-cancer-associated-thrombosis
#19
Ana C Leal, Daniella M Mizurini, Tainá Gomes, Natalia C Rochael, Elvira M Saraiva, Marcos S Dias, Claudio C Werneck, Micheli S Sielski, Cristina P Vicente, Robson Q Monteiro
Cancer patients are at an increased risk of developing thromboembolic complications. Several mechanisms have been proposed to explain cancer-associated thrombosis including the release of tumor-derived extracellular vesicles and the activation of host vascular cells. It was proposed that neutrophil extracellular traps (NETs) contribute to the prothrombotic phenotype in cancer. In this study, we evaluated the possible cooperation between tumor-derived exosomes and NETs in cancer-associated thrombosis. Female BALB/c mice were orthotopically injected with 4T1 breast cancer cells...
July 25, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28740236/reduced-expression-of-the-murine-hla-g-homolog-qa-2-is-associated-with-malignancy-epithelial-mesenchymal-transition-and-stemness-in-breast-cancer-cells
#20
Istéfani L da Silva, Lucía Montero-Montero, Ester Martín-Villar, Jorge Martin-Pérez, Bruno Sainz, Jaime Renart, Renata Toscano Simões, Émerson Soares Veloso, Cláudia Salviano Teixeira, Mônica C de Oliveira, Enio Ferreira, Miguel Quintanilla
Qa-2 is believed to mediate a protective immune response against cancer; however, little is known about the role of Qa-2 in tumorigenesis. Here, we used 4T1 breast cancer cells to study the involvement of Qa-2 in tumor progression in a syngeneic host. Qa-2 expression was reduced during in vivo tumor growth and in cell lines derived from 4T1-induced tumors. Tumor-derived cells elicited an epithelial-mesenchymal transition associated with upregulation of Zeb1 and Twist1/2 and enhanced tumor initiating and invasive capacities...
July 24, 2017: Scientific Reports
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