keyword
MENU ▼
Read by QxMD icon Read
search

4T1

keyword
https://www.readbyqxmd.com/read/28723583/label-free-high-resolution-3-d-imaging-of-gold-nanoparticles-inside-live-cells-using-optical-diffraction-tomography
#1
Doyeon Kim, Nuri Oh, Kyoohyun Kim, SangYun Lee, Chan-Gi Pack, Ji-Ho Park, YongKeun Park
Delivery of gold nanoparticles (GNPs) into live cells has high potentials, ranging from molecular-specific imaging, photodiagnostics, to photothermal therapy. However, studying the long-term dynamics of cells with GNPs using conventional fluorescence techniques suffers from phototoxicity and photobleaching. Here, we present a method for 3-D imaging of GNPs inside live cells exploiting refractive index (RI) as imaging contrast. Employing optical diffraction tomography, 3-D RI tomograms of live cells with GNPs are precisely measured for an extended period with sub-micrometer resolution...
July 16, 2017: Methods: a Companion to Methods in Enzymology
https://www.readbyqxmd.com/read/28713159/co-delivery-of-docetaxel-and-silibinin-using-ph-sensitive-micelles-improves-therapy-of-metastatic-breast-cancer
#2
Xin-Yue Dong, Tian-Qun Lang, Qi Yin, Peng-Cheng Zhang, Ya-Ping Li
Breast cancer is the most vicious killer for women, and tumor metastasis is one of the leading causes of breast cancer therapy failure. In this study, a new pH-sensitive polymer (polyethylene glycol-block-poly[(1,4-butanediol)-diacrylate-β-N,N-diisopropylethylenediamine], BDP) was synthesized. Based on BDP, docetaxel/silibinin co-delivery micelles (DSMs) was constructed. DSM had a well-defined spherical shape under the transmission electron microscope with average hydrodynamic diameter of 85.3±0.4 nm, and were stable in the bloodstream but could dissociate to release the chemotherapeutic agents in the low pH environment of the endo/lysosomes in the tumor cells...
July 17, 2017: Acta Pharmacologica Sinica
https://www.readbyqxmd.com/read/28703043/acid-sensitive-lipidated-doxorubicin-prodrug-entrapped-in-nanoemulsion-impairs-lung-tumor-metastasis-in-a-breast-cancer-model
#3
Ana Lygia Dos Santos Câmara, Gregor Nagel, Harald R Tschiche, Camila Magalhães Cardador, Luis Alexandre Muehlmann, Daniela Mara de Oliveira, Paula Queiroz Alvim, Ricardo Bentes Azevedo, Marcelo Calderón, João Paulo Figueiró Longo
AIM: To develop an acid-sensitive lipidated, doxorubicin (Dox) prodrug (C16-Dox) to be entrapped in lipid nanoemulsion (NE-C16-Dox) as a nanocarrier to treat breast cancer models (in vitro and in vivo). RESULTS: We report the efficacy of NE-C16-Dox in in vitro experiments, as well as the improved chemotherapeutic index and tumor-control efficacy compared with treatment with free Dox in an in vivo murine 4T1 breast cancer model. In addition, NE-C16-Dox allowed the use of a higher dose of Dox, acceptable biocompatibility and a significant reduction in lung metastasis...
July 13, 2017: Nanomedicine
https://www.readbyqxmd.com/read/28702658/overcoming-multidrug-resistance-using-folate-receptor-targeted-and-ph-responsive-polymeric-nanogels-containing-covalently-entrapped-doxorubicin
#4
Y Chen, O Tezcan, D Li, N Beztsinna, B Lou, T Etrych, K Ulbrich, J M Metselaar, T Lammers, W E Hennink
Multidrug resistance (MDR) contributes to failure of chemotherapy. We here show that biodegradable polymeric nanogels are able to overcome MDR via folic acid targeting. The nanogels are based on hydroxyethyl methacrylamide-oligoglycolates-derivatized poly(hydroxyethyl methacrylamide-co-N-(2-azidoethyl)methacrylamide) (p(HEMAm-co-AzEMAm)-Gly-HEMAm), covalently loaded with the chemotherapeutic drug doxorubicin (DOX) and subsequently decorated with a folic acid-PEG conjugate via copper-free click chemistry. pH-Responsive drug release is achieved via the acid-labile hydrazone bond between DOX and the methacrylamide polymeric network...
July 12, 2017: Nanoscale
https://www.readbyqxmd.com/read/28700822/laser-desorption-postionization-mass-spectrometry-imaging-of-folic-acid-molecules-in-tumor-tissue
#5
Qiao Lu, Yongjun Hu, Jiaxin Chen, Shan Jin
Mass spectrometry imaging (MSI) is an innovative and powerful tool in biomedical research. It is well known that folic acid (FA) has a high affinity for folic acid receptor (FR) which is overexpressing in epithelial cancer. Herein, we propose a novel method to diagnose cancer through direct mapping of the label-free FA spatial distribution in tissue sections by state-of-the-art laser desorption postionization mass spectrometry imaging (LDPI-MSI). Compared with other tumor imaging methods, such as fluorescence imaging, photoacoustic imaging (PAI), magnetic resonance imaging (MRI) and micro-SPECT/CT, complicated synthesis and labeling processes are not required...
July 12, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28700135/a-hybrid-clustering-algorithm-for-multiple-source-resolving-in-bioluminescence-tomography
#6
Hongbo Guo, Jingjing Yu, Zhenhua Hu, Huangjian Yi, Yuqing Hou, Xiaowei He
Bioluminescence tomography is a preclinical imaging modality to locate and quantify internal bioluminescent sources from surface measurements, which experienced rapid growth in the last ten years. However, multiple-source resolving remains a challenging issue in BLT. In this paper, it is treated as an unsupervised pattern recognition problem based on the reconstruction result, and a novel hybrid clustering algorithm combining the advantages of affinity propagation (AP) and K-means is developed to identify multiple sources automatically...
July 12, 2017: Journal of Biophotonics
https://www.readbyqxmd.com/read/28690055/antitumor-effect-of-ferula-assa-foetida-oleo-gum-resin-against-breast-cancer-induced-by-4t1-cells-in-balb-c-mice
#7
Seyyed Majid Bagheri, Amir Abdian-Asl, Mahin Taheri Moghadam, Maryam Yadegari, Aghdas Mirjalili, Fatemeh Zare-Mohazabieh, Haniyeh Momeni
BACKGROUND: Ferula assa foetida commonly consumed as a healthy beverage has been demonstrated to have various biological activities, including antioxidation, anti-obesity and anti-cancer. OBJECTIVE: Our study aims to investigate the antitumor effect of asafoetida in vivo using mouse mammary carcinoma 4T1 cells. MATERIALS AND METHODS: In the study, female BALB/c mice were divided into two groups (n = 6), which were control (untreated) and other group of mice with breast cancer treated with 100 mg/kg of asafoetida, respectively, by oral gavage...
July 6, 2017: Journal of Ayurveda and Integrative Medicine
https://www.readbyqxmd.com/read/28687620/kindlin-2-regulates-the-growth-of-breast-cancer-tumors-by-activating-csf-1-mediated-macrophage-infiltration
#8
Khalid Sossey-Alaoui, Elzbieta Pluskota, Katarzyna Bialkowska, Dorota Szpak, Yvonne Parker, Chevaun Morrison, Daniel J Lindner, William P Schiemann, Edward F Plow
Interplay between tumor cells and host cells in the tumor microenvironment dictates the development of all cancers. In breast cancer, malignant cells educate host macrophages to adopt a pro-tumorigenic phenotype. In this study, we show how the integrin regulatory protein kindlin-2 (FERMT2) promotes metastatic progression of breast cancer through the recruitment and subversion of host macrophages. Kindlin-2 expression was elevated in BC biopsy tissues where its levels correlated with reduced patient survival...
July 7, 2017: Cancer Research
https://www.readbyqxmd.com/read/28686414/stimuli-responsive-cluster-bomb-for-programmed-tumor-therapy
#9
Qi Lei, Shi-Bo Wang, Jing-Jing Hu, Yi-Xiong Lin, Cheng-Hui Zhu, Lei Rong, Xian-Zheng Zhang
In this paper, mesoporous silica nanoparticle (MSN) loaded with doxorubicin (DOX) and capped with tumor homing/penetrating peptide tLyP-1-modified tungsten disulfide quantum dots (WS2-HP) was designed and applied as a stimuli-responsive "Cluster Bomb" for high-performance tumor suppression. The peptide tLyP-1 on the surface can both facilitate the homing of DOX@MSN-WS2-HP to 4T1 tumor and greatly enhance the penetration of WS2-HP in tumor. The benzoic-imine bonds as the linkers between "bomblets" and "dispenser" are stable under normal physical conditions and quite labile at pH 6...
July 7, 2017: ACS Nano
https://www.readbyqxmd.com/read/28683649/in-vitro-and-in-vivo-delivery-of-artemisinin-loaded-pcl-peg-pcl-micelles-and-its-pharmacokinetic-study
#10
Hamidreza Kheiri Manjili, Hojjat Malvandi, Mir Sajjad Mousavi, Elahe Attari, Hossein Danafar
Artemisinin (ART) is a natural anti-malarial sesquiterpene lactone with anticancer properties, but its application is limited because of its low water solubility. To increase the bioavailability and water solubility of ART, we synthesized three series of poly (ɛ-caprolactone)-poly (ethylene glycol)-poly (ɛ-caprolactone) (PCL-PEG-PCL) tri-block copolymers. The structure of the copolymers was characterized by HNMR, FTIR, DSC and GPC techniques. ART was encapsulated inside micelles by a nanoprecipitation method which leading to the formation of ART/PCL-PEG-PCL micelles...
July 7, 2017: Artificial Cells, Nanomedicine, and Biotechnology
https://www.readbyqxmd.com/read/28680747/arginase-inhibition-suppresses-lung-metastasis-in-the-4t1-breast-cancer-model-independently-of-the-immunomodulatory-and-anti-metastatic-effects-of-vegfr-2-blockade
#11
Chiara Secondini, Oriana Coquoz, Lorenzo Spagnuolo, Thibaud Spinetti, Sanam Peyvandi, Laura Ciarloni, Francesca Botta, Carole Bourquin, Curzio Rüegg
Tumor angiogenesis promotes tumor growth and metastasis. Anti-angiogenic therapy in combination with chemotherapy is used for the treatment of metastatic cancers, including breast cancer but therapeutic benefits are limited. Mobilization and accumulation of myeloid-derived suppressor cells (MDSC) during tumor progression and therapy have been implicated in metastasis formation and resistance to anti-angiogenic treatments. Here, we used the 4T1 orthotopic syngenic mouse model of mammary adenocarcinoma to investigate the effect of VEGF/VEGFR-2 axis inhibition on lung metastasis, MDSC and regulatory T cells (Tregs)...
2017: Oncoimmunology
https://www.readbyqxmd.com/read/28677607/design-synthesis-crystal-structure-and-in-vitro-cytotoxic-properties-of-a-novel-schiff-base-derived-from-indole-and-biphenyl
#12
Fan Zhi Bu, Xue Jie Tan, Dian Xiang Xing, Chao Wang
A novel and potentially active dihydroorotate dehydrogenase (DHODH) inhibitor, namely 3-({(E)-[(E)-1-(biphenyl-4-yl)ethylidene]hydrazinylidene}methyl)-1H-indole (BEHI) acetonitrile disolvate, C23H19N3·2CH3CN, has been designed and synthesized. The structure of BEHI was characterized by elemental analysis, Q-TOF (quadrupole time-of-flight) MS, NMR, UV-Vis and single-crystal X-ray diffraction. The antitumour activity of the target molecule was evaluated by the MTT method. Results indicated that BEHI exhibited rather potent cytotoxic activity against human A549 (IC50 = 20...
July 1, 2017: Acta Crystallographica. Section C, Structural Chemistry
https://www.readbyqxmd.com/read/28672849/synergy-of-iron-chelators-and-therapeutic-peptide-sequences-delivered-via-a-magnetic-nanocarrier
#13
Gayani S Abayaweera, Hongwang Wang, Tej B Shrestha, Jing Yu, Kyle Angle, Prem Thapa, Aruni P Malalasekera, Leila Maurmann, Deryl L Troyer, Stefan H Bossmann
Here, we report the synthesis, characterization, and efficacy study of Fe/Fe₃O₄-nanoparticles that were co-labeled with a tumor-homing and membrane-disrupting oligopeptide and the iron-chelator Dp44mT, which belongs to the group of the thiosemicarbazones. Dp44mT and the peptide sequence PLFAERL(D[KLAKLAKKLAKLAK])CGKRK were tethered to the surface of Fe/Fe₃O₄ core/shell nanoparticles by utilizing dopamine-anchors. The 26-mer contains two important sequences, which are the tumor targeting peptide CGKRK, and D[KLAKLAK]₂, known to disrupt the mitochondrial cell walls and to initiate programmed cell death (apoptosis)...
June 26, 2017: Journal of Functional Biomaterials
https://www.readbyqxmd.com/read/28661654/matrix-metalloproteinase-responsive-multifunctional-peptide-linked-amphiphilic-block-copolymers-for-intelligent-systemic-anticancer-drug-delivery
#14
Wendong Ke, Zengshi Zha, Jean Felix Mukerabigwi, Weijian Chen, Yuheng Wang, Chuanxin He, Zhishen Ge
The amphiphilic block copolymer anticancer drug nanocarriers clinically used or in the progress of clinical trials frequently suffer from the modest final therapeutic efficacy due to lack of intelligent features. For example, the biodegradable amphiphilic block copolymer, poly(ethylene glycol)-b-poly(D,L-lactide) (PEG-PDLLA), has been approved for clinical applications as a paclitaxel (PTX) nanocarrier (Genexol-PM) due to the optimized pharmacokinetics and biodistribution, however, lack of intelligent features limits the intracellular delivery in tumor tissue...
June 29, 2017: Bioconjugate Chemistry
https://www.readbyqxmd.com/read/28659032/epimedin-c-promotes-vascularization-during-bmp2-induced-osteogenesis-and-tumor-associated-angiogenesis
#15
Yan-Mei Shui, Gui-Yuan Lv, Le-Tian Shan, Chun-Lei Fan, Nan Tian, Li Zhang, Tong-Chuan He, Jian-Li Gao
Epimedin C is one of the chemical markers and major flavonoids in Herba Epimedii (Yinyanghuo), which is traditionally used to treat bone diseases and gonadal dysfunction in China. Our previous study indicated that epimedin C could induce endothelial-like, but not osteogenic differentiation of C3H/10T1/2 cells in vitro. As vasculogenesis plays a pivotal role in bone formation, this study used the bone morphogenetic protein 2 (BMP2) induced ectopic bone formation model and mice 4T1 breast cancer cells co-implanted with luciferase labeled C3H/10T1/2 cells (4T1 [Formula: see text] C3H/10T1/2-Luc) model to examine the in vivo effects of Epimedin C on vasculogenesis...
June 28, 2017: American Journal of Chinese Medicine
https://www.readbyqxmd.com/read/28656751/gadolinium-labeled-biodegradable-dendron-hyaluronic-acid-hybrid-and-its-subsequent-application-as-a-safe-and-efficient-magnetic-resonance-imaging-contrast-agent
#16
Chunhua Guo, Ling Sun, Hao Cai, Zhenyu Duan, Shiyong Zhang, Qiyong Gong, Kui Luo, Zhongwei Gu
Novel magnetic resonance imaging (MRI) contrast agents with high sensitivity and good biocompatibility are required for the diagnosis of cancer. Herein, we prepared and characterized the gadolinium [Gd(III)]-labeled peptide dendron-hyaluronic acid (HA) conjugate-based hybrid (dendronized-HA-DOTA-Gd) by combining the advantages of HA and the peptide dendron. The dendronized-HA-DOTA-Gd hybrid with 3.8% Gd(III) as weight percentage showed a negative zeta potential (-35 mV). The in vitro degradation results indicated that the dendronized-HA-DOTA-Gd hybrid degraded into products with low molecular weights in the presence of hyaluronidase...
July 19, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28649130/dopamine-d2-receptor-antagonist-sulpiride-enhances-dexamethasone-responses-in-the-treatment-of-drug-resistant-and-metastatic-breast-cancer
#17
Jian Li, Qing-Yu Yao, Jun-Sheng Xue, Li-Jie Wang, Yin Yuan, Xiu-Yun Tian, Hong Su, Si-Yuan Wang, Wen-Jun Chen, Wei Lu, Tian-Yan Zhou
Recent evidence shows that dopamine D2-like receptor (D2DR) antagonists, such as trifluoperazine and thioridazine, are effective for cancer therapy and inhibition of cancer stem-like cells (CSCs). In this study, we investigated the anti-cancer effects of combination therapy of dexamethasone (DEX) and sulpiride (SUL), an atypical antipsychotic, against drug-resistant and metastatic breast cancers and further explored the underlying mechanisms. Oral administration of SUL (25, 100 mg·kg(-1)·d(-1)) alone did not inhibit the tumor growth in human breast cancer MCF-7/Adr xenograft model, but dose-dependently decreased the proportion of CSCs in vitro and in vivo...
June 26, 2017: Acta Pharmacologica Sinica
https://www.readbyqxmd.com/read/28642204/co-delivery-of-paclitaxel-and-anti-survivin-sirna-via-redox-sensitive-oligopeptide-liposomes-for-the-synergistic-treatment-of-breast-cancer-and-metastasis
#18
Xinyan Chen, Yidi Zhang, Chunming Tang, Chunli Tian, Qiong Sun, Zhigui Su, Lingjing Xue, Yifan Yin, Caoyun Ju, Can Zhang
The overexpression of survivin in breast cancer cells is an important factor of paclitaxel (PTX) resistance in breast cancer. To overcome PTX resistance and improve the antitumor effect of PTX, we developed a novel liposome-based nanosystem (PTX/siRNA/SS-L), composed of a redox-sensitive cationic oligopeptide lipid (LHSSG2C14) with a proton sponge effect, natural soybean phosphatidylcholine (SPC), and cholesterol for co-delivery of PTX and anti-survivin siRNA, which could specifically downregulate survivin overexpression...
June 19, 2017: International Journal of Pharmaceutics
https://www.readbyqxmd.com/read/28640347/psychotria-viridis-chemical-constituents-from-leaves-and-biological-properties
#19
Débora B S Soares, Lucienir P Duarte, André D Cavalcanti, Fernando C Silva, Ariadne D Braga, Miriam T P Lopes, Jacqueline A Takahashi, Sidney A Vieira-Filho
The phytochemical study of hexane, chloroform and methanol extracts from leaves of Psychotria viridis resulted in the identification of: the pentacyclic triterpenes, ursolic and oleanolic acid; the steroids, 24-methylene-cycloartanol, stigmasterol and β-sitosterol; the glycosylated steroids 3-O-β-D-glucosyl-β-sitosterol and 3-O-β-D-glucosyl-stigmasterol; a polyunsaturated triterpene, squalene; the esters of glycerol, 1-palmitoylglycerol and triacylglycerol; a mixture of long chain hydrocarbons; the aldehyde nonacosanal; the long chain fat acids hentriacontanoic, hexadecanoic and heptadenoic acid; the ester methyl heptadecanoate; the 4-methyl-epi-quinate and two indole alkaloids, N,N-dimethyltryptamine (DMT) and N-methyltryptamine...
April 2017: Anais da Academia Brasileira de Ciências
https://www.readbyqxmd.com/read/28638724/calcium-electroporation-induces-tumor-eradication-long-lasting-immunity-and-cytokine-responses-in-the-ct26-colon-cancer-mouse-model
#20
Hanne Falk, Patrick F Forde, Marie Lund Bay, Uma Maheswari Mangalanathan, Pernille Hojman, Declan M Soden, Julie Gehl
Electroporation is used in cancer treatment because of its ability to increase local cytotoxicity of e.g. bleomycin (electrochemotherapy) and calcium (calcium electroporation). Calcium electroporation is a novel anticancer treatment that selectively kills cancer cells by necrosis, a cell death pathway that stimulates the immune system due to high release of antigens and "danger signals." In this exploratory study, we aimed to investigate whether calcium electroporation could initiate an anticancer immune response similar to electrochemotherapy...
2017: Oncoimmunology
keyword
keyword
37232
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"