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R Xue, Y Tian, T Hou, D Bao, H Chen, Q Teng, J Yang, X Li, G Wang, Z Li, Q Liu
H9N2 is one of the major subtypes of influenza virus circulating in poultry in China, which has a wide host range from bird to mammals. Two H9N2 viruses were isolated from one mink farm in 2014. Phylogenetic analysis showed that internal genes of the H9N2 viruses have close relationship with those of H7N9 viruses. Interestingly, two H9N2 were separated in phylogenetic trees, indicating that they are introduced to this mink farm in two independent events. And further mice studies showed that one H9N2 caused obvious weight loss and 20% mortality in infected mice, while another virus did not cause any clinical sign in mice infected at the same dose...
January 14, 2018: Transboundary and Emerging Diseases
Ying-Feng Chang, Wen-Hung Wang, Yi-Wei Hong, Ruei-Yu Yuan, Kuan-Hsuan Chen, Yu-Wen Huang, Po-Liang Lu, Yen-Hsu Chen, Yi-Ming Arthur Chen, Li-Chen Su, Sheng-Fan Wang
A new reassortant avian influenza A H7N9 virus have emerged in China since 2013, causing human infection with high mortality. An accurate and timely diagnosis is crucial for controlling the outbreaks of the disease. We therefore propose a simple strategy for rapidly and sensitively detecting the H7N9 virus using an intensity-modulated surface plasmon resonance (IM-SPR) biosensor integrated with a new generated monoclonal antibody. The novel antibody exhibits significant specificity to recognize H7N9 virus compared with other clinical human influenza isolates (p<0...
January 12, 2018: Analytical Chemistry
David S Hui, Nelson Lee, Paul K Chan, John H Beigel
A severe inflammatory immune response with hypercytokinemia occurs in patients hospitalized with severe influenza, such as avian influenza A(H5N1), A(H7N9), and seasonal A(H1N1)pdm09 virus infections. The role of immunomodulatory therapy is unclear as there have been limited published data based on randomized controlled trials (RCTs). Passive immunotherapy such as convalescent plasma and hyperimmune globulin have some studies demonstrating benefit when administered as an adjunctive therapy for severe influenza...
January 8, 2018: Antiviral Research
Liuluan Zhu, Lu Liu, Yue Zhang, Lin Pu, Jingyuan Liu, Xingwang Li, Zhihai Chen, Yu Hao, Beibei Wang, Junyan Han, Guoli Li, Shuntao Liang, Haofeng Xiong, Hong Zheng, Ang Li, Jianqing Xu, Hui Zeng
Background: Most patients with severe infection with influenza A virus (IAV) progress to acute respiratory distress syndrome and even multiple organ dysfunction syndrome (MODS). Neutrophil extracellular traps (NETs) can be induced by pathogens and are responsible for immune tissue damage. We conducted a prospective study on the production and effects of NETs in H7N9 and H1N1 patients. Methods: We investigated NET production in plasma and supernatant of cultured neutrophils by measuring cell-free deoxyribonucleic acid (DNA) and myeloperoxidase (MPO)-DNA complexes with PicoGreen dye and enzyme-linked immunosorbent assay methods, respectively...
January 9, 2018: Journal of Infectious Diseases
Victor Virlogeux, Luzhao Feng, Tim K Tsang, Hui Jiang, Vicky J Fang, Ying Qin, Peng Wu, Xiling Wang, Jiandong Zheng, Eric H Y Lau, Zhibin Peng, Juan Yang, Benjamin J Cowling, Hongjie Yu
A novel avian-origin influenza A(H7N9) virus emerged in China in March 2013 and by 27 September 2017 a total of 1533 laboratory-confirmed cases have been reported. Occurrences of animal-to-human and human-to-human transmission have been previously identified, and the force of human-to-human transmission is an important component of risk assessment. In this study, we constructed an ecological model to evaluate the animal-to-human and human-to-human transmission of H7N9 during the first three epidemic waves in spring 2013, winter/spring 2013-2014 and winter/spring 2014-2015 in China based on 149 laboratory-confirmed urban cases...
January 11, 2018: Scientific Reports
Yingying Cao, Yaowei Huang, Ke Xu, Yuanhua Liu, Xuan Li, Ye Xu, Wu Zhong, Pei Hao
BACKGROUND: Innate immunity provides first line of defense against viral infections. The interactions between hosts and influenza A virus and the response of host innate immunity to viral infection are critical determinants for the pathogenicity or virulence of influenza A viruses. This study was designed to investigate global changes of gene expression and detailed responses of innate immune systems in human and avian hosts during the course of infection with various subtypes of influenza A viruses, using collected and self-generated transcriptome sequencing data from human bronchial epithelial (HBE), human tracheobronchial epithelial (HTBE), and A549 cells infected with influenza A virus subtypes, namely H1N1, H3N2, H5N1 HALo mutant, and H7N9, and from ileum and lung of chicken and quail infected with H5N1, or H5N2...
December 21, 2017: BMC Medical Genomics
David S C Hui, Nelson Lee, Paul K S Chan
No abstract text is available yet for this article.
December 2017: Journal of Thoracic Disease
Linna Huang, Wei Zhang, Yi Yang, Wenjuan Wu, Weihua Lu, Han Xue, Hongsheng Zhao, Yunfu Wu, Jia Shang, Lihua Cai, Long Liu, Donglin Liu, Yeming Wang, Bin Cao, Qingyuan Zhan, Chen Wang
No abstract text is available yet for this article.
January 8, 2018: BMC Infectious Diseases
Ling Wang, Xiaobin Fang, Yongling Yang, Zhengping Zhang, Jianlin Zhou, Jingsong Yang, Kaifeng Liu, Zhenhua Wang
OBJECTIVE: To design bundle treatment plan in the early stage for severe human infection by avian influenza H7N9, and explore its clinical efficacy and application value. METHODS: Fifteen patients with severe human infection by avian influenza H7N9 in Guizhou Province from December 29th, 2016 to June 7th, 2017 were enrolled. Patients admitted from March 6th, 2017 to June 7th, 2017 served as a prospective observation period (bundle treatment group), and those from December 29th, 2016 to March 5th, 2017 were selected as a historical control period (conventional treatment group)...
January 2018: Zhonghua Wei Zhong Bing Ji Jiu Yi Xue
Liqi Liu, Jian Lu, Zi Li, Jianfang Zhou, Junfeng Guo, Xiyan Li, Jia Liu, Yuelong Shu, Dayan Wang
Since the first confirmed case of H7N9 infection was reported in China, there have been five epidemic waves of human H7N9 infections between 2013 and 2017. The fifth wave differed from the previous four waves in that highly pathogenic avian influenza (HPAI) H7N9 viruses with multiple basic amino acids at the cleavage site were detected in humans, poultry and environmental samples. The HPAI H7N9 viruses were genetically and antigenically distinct from previous H7N9 viruses. Therefore, a new candidate vaccine virus(CVV) derived from a HPAI A/Guangdong/17SF003/2016-like virus was proposed by the World Health Organization(WHO)...
January 4, 2018: Human Vaccines & Immunotherapeutics
T Liu, G H Zhu, B Zhang, T Song, M Kang, J Lu, Y Q Zhao, Z Huang, Y L Huang, X J Wang, X Y Yang, W J Ma
Since March 2013, China had experienced five seasonal epidemics related to Avian influenza A (H7N9). An unprecedented outbreak of H7N9 epidemic started from September 2016, with 730 cases reported till June 30(th) 2017, in mainland China that caused profound influences on both social development and health of the people. As an emerging infectious disease, information on pathogenic characteristics, transmission patterns and other epidemiological features of H7N9 virus somehow remained unclear. Data from previous studies suggested that the live poultry market (LPM) seemed to have served as main places where H7N9 virus got originated, mutated, spread and thus infected the human beings...
December 10, 2017: Zhonghua Liu Xing Bing Xue za Zhi, Zhonghua Liuxingbingxue Zazhi
Jean Artois, Hui Jiang, Xiling Wang, Ying Qin, Morgan Pearcy, Shengjie Lai, Yujing Shi, Juanjuan Zhang, Zhibin Peng, Jiandong Zheng, Yangni He, Madhur S Dhingra, Sophie von Dobschuetz, Fusheng Guo, Vincent Martin, Wantanee Kalpravidh, Filip Claes, Timothy Robinson, Simon I Hay, Xiangming Xiao, Luzhao Feng, Marius Gilbert, Hongjie Yu
The fifth epidemic wave of avian influenza A(H7N9) virus in China during 2016-2017 demonstrated a geographic range expansion and caused more human cases than any previous wave. The factors that may explain the recent range expansion and surge in incidence remain unknown. We investigated the effect of anthropogenic, poultry, and wetland variables on all epidemic waves. Poultry predictor variables became much more important in the last 2 epidemic waves than they were previously, supporting the assumption of much wider H7N9 transmission in the chicken reservoir...
January 2018: Emerging Infectious Diseases
Jessica A Belser, Nicole Brock, Xiangjie Sun, Joyce Jones, Natosha Zanders, Erin Hodges, Joanna A Pulit-Penaloza, David Wentworth, Terrence M Tumpey, Todd Davis, Taronna R Maines
Infections with low pathogenicity and highly pathogenic avian influenza A(H7N9) viruses affected poultry in 4 states in the southeastern United States in 2017. We evaluated pathogenicity and transmission of representative viruses in mouse and ferret models and examined replication kinetics in human respiratory tract cells. These viruses can cause respiratory infections in mammalian models.
January 2018: Emerging Infectious Diseases
Zhen Wu, Wen-Jing Guo, Yi-Yan Bai, Li Zhang, Jiao Hu, Dai-Wen Pang, Zhi-Ling Zhang
Electrochemistry has been widely used to explore fundamental properties of single molecules due to its fast response and high specificity. However, the lack of efficient signal amplification strategies and quantitative method limit its clinical application. Here, we proposed a digital single virus electrochemical enzyme-linked immunoassay (digital ELISA) for H7N9 avian influenza virus (H7N9 AIV) counting by integration of digital analysis, bifunctional fluorescence magnetic nanospheres (bi-FMNs) with monolayer gold nanoparticles (Au NPs) modified microelectrode array (MA)...
December 20, 2017: Analytical Chemistry
Hai-Feng Lu, Ang Li, Ting Zhang, Zhi-Gang Ren, Kang-Xin He, Hua Zhang, Jie-Zuan Yang, Qi-Xia Luo, Kai Zhou, Chun-Lei Chen, Xia-Liang Chen, Zhong-Wen Wu, Lan-Juan Li
Secondary bacterial lung infection (SBLI) is a serious complication in patients with H7N9 virus infection, and increases disease severity. The oropharyngeal (OP) microbiome helps prevent colonisation of respiratory pathogens. We aimed to investigate the OP microbiome of H7N9 patients with/without secondary bacterial pneumonia using 16S rRNA gene sequencing. OP swab samples were collected from 51 H7N9 patients (21 with SBLI and 30 without) and 30 matched healthy controls (HCs) and used for comparative composition, diversity and richness analyses of microbial communities...
December 20, 2017: Emerging Microbes & Infections
Seiya Yamayoshi, Mutsumi Ito, Ryuta Uraki, Tadahiro Sasaki, Kazuyoshi Ikuta, Yoshihiro Kawaoka
OBJECTIVES: Broadly reactive human monoclonal antibodies against the HA stem of influenza A virus are being developed as therapeutic agents as well as to understand the epitopes that are essential for a universal influenza virus vaccine. METHODS: We isolated and characterized two hetero-reactive human monoclonal antibodies from an H3N2 virus-infected human. RESULTS: These antibodies, which are predominantly bound to the HA stem of group 2 HAs, used IGHV3-66 and IGHV4-38-2 germline genes, respectively...
December 14, 2017: Journal of Infection
Shuangsheng Wu, Jianting Su, Peng Yang, Haiyan Zhang, Hongjun Li, Yanhui Chu, Weiyu Hua, Chao Li, Yaqing Tang, Quanyi Wang
BACKGROUND: The present study aimed to estimate residents' willingness to accept a future H7N9 vaccine and its determinants in the general adult population in Beijing, China. METHODS: We conducted a multi-stage sampling, cross-sectional survey using self-administered anonymous questionnaires from May to June, in 2014. The main outcome variable was residents' willingness to accept a future H7N9 vaccine. Logistic regression was used to identify the predictors of vaccination willingness...
December 12, 2017: Vaccine
Jose M Trevejo, Mohammed Asmal, Johan Vingerhoets, Ramon Polo, Sarah Robertson, Ying Jiang, Tara L Kieffer, Lorant Leopold
BACKGROUND: JNJ-63623872 is a novel, non-nucleoside polymerase complex inhibitor with in vitro activity against influenza A virus, including pandemic 2009 H1N1, H7N9, H5N1 strains as well as neuraminidase-and amantadine-resistant strains. METHODS: Randomized, double-blind, placebo-controlled, Phase 2a study. Healthy volunteers (N = 104) were inoculated with an influenza A/Wisconsin/67/2005 (H3N2) challenge virus. 72 received JNJ-63623872 and 32 placebo. JNJ-63623872 was dosed for 5 days once daily from 24 hours after viral inoculation at four dose levels: 100 mg, 400 mg, loading dose 900/600 mg and loading dose 1200/600 mg...
December 15, 2017: Antiviral Therapy
Wei Cheng, Xiaoxiao Wang, Ye Shen, Zhao Yu, Shelan Liu, Jian Cai, Enfu Chen
BACKGROUND: The sudden increase of the number of human cases infected with avian influenza A(H7N9) virus after September, 2016 raised global concern. OBJECTIVES: To assess the changes of epidemiological characteristics of H7N9 cases since the massive closure of live poultry markets (LPMs)in the main urban areas in Zhejiang province. METHODS: We used descriptive statistics to compare epidemiological characteristics of the three distinct waves of H7N9 cases in Zhejiang province...
December 15, 2017: Influenza and Other Respiratory Viruses
Daniel Stadlbauer, Arvind Rajabhathor, Fatima Amanat, Daniel Kaplan, Abusaleh Masud, John J Treanor, Ruvim Izikson, Manon M Cox, Raffael Nachbagauer, Florian Krammer
Human influenza virus infections with avian subtype H7N9 viruses are a major public health concern and have encouraged the development of effective H7 prepandemic vaccines. In this study, baseline and postvaccination serum samples of individuals aged 18 years and older who received a recombinant H7 hemagglutinin vaccine with and without an oil-in-water emulsion (SE) adjuvant were analyzed using a panel of serological assays. While only a small proportion of individuals seroconverted to H7N9 as measured by the conventional hemagglutination inhibition assay, our data show strong induction of anti-H7 hemagglutinin antibodies as measured by an enzyme-linked immunosorbent assay (ELISA)...
November 2017: MSphere
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