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Jacqueline Montanaro, Daniela Gruber, Nikolaus Leisch
Many marine biology studies depend on field work on ships or remote sampling locations where sophisticated sample preservation techniques (e.g., high-pressure freezing) are often limited or unavailable. Our aim was to optimize the ultrastructural preservation of marine invertebrates, especially when working in the field. To achieve chemically-fixed material of the highest quality, we compared the resulting ultrastructure of gill tissue of the mussel Mytilus edulis when fixed with differently buffered EM fixatives for marine specimens (seawater, cacodylate and phosphate buffer) and a new fixative formulation with the non-toxic PHEM buffer (PIPES, HEPES, EGTA and MgCl2)...
2016: PeerJ
William H Seligman, Sameer Ganatra, David England, John J M Black
Prehospital emergency medicine (PHEM) is a recently recognised subspecialty of emergency medicine, and anaesthetics, intensive care and acute medicine, in the UK, and yet it receives little to no mention in many undergraduate medical curricula. However, there is growing interest in PHEM among medical students and junior doctors. Several programmes are in existence across the UK that serve to provide teaching and exposure of prehospital care to medical students and junior doctors. However, relatively few students are able to gain significant first-hand experience of treating patients in the prehospital phase...
February 2016: Emergency Medicine Journal: EMJ
Leila Bassir, Linda Dykes, Rob Perry
INTRODUCTION: We noticed that COPD patients travelling long distances to our rural ED arrived in a worse condition than those living nearby. We suspected this might be due to oxygen-driven nebulisers en route: high-flow oxygen during transfer is known to increase COPD mortality. METHODS: We conducted a retrospective chart review of ED patients who arrived by ambulance and were admitted with exacerbations of COPD December 2010-March 2011. ▸ 42/114 lacked ambulance records, leaving 72 cases for analysis...
May 2015: Emergency Medicine Journal: EMJ
Vinicio Carias, Jing Wang, Ryan Toomey
A series of terpolymers were synthesized comprising the following monomers: N-isopropylacrylamide (NIPAAm), a stimuli-responsive structural unit that swells and collapses in response to temperature; methacryloxybenzophenone (MaBP), a photo-cross-linking unit that is activated at a wavelength of 365 nm; and phenacyl methacrylate (PHEm), a photolabile protected carboxyl group that can be deprotected at a wavelength of 254 nm. It is shown that the terpolymers can be photo-cross-linked at long UV wavelength light (λ = 365 nm) to establish surface-attached, cross-linked coatings and subsequently photochemically cleaved at short UV wavelength light (λ = 254 nm), which is found to be consistent with first-order kinetics...
April 15, 2014: Langmuir: the ACS Journal of Surfaces and Colloids
Tzipora Strauss, Naomi Rozenzweig, Nurit Rosenberg, Boris Shenkman, Tami Livnat, Iris Morag, Yariv Fruchtman, Uri Martinowitz, Gili Kenet
BACKGROUND: Pulmonary hemorrhage (PHEM) complicates the hospital course of 3-5% of preterm infants with respiratory distress syndrome (RDS), and bears a high mortality rate. Impaired thrombin generation and poor clot formation in premature neonates affect PHEM severity. OBJECTIVES: We evaluated the impact of surfactant upon in-vitro clot formation in order to assess the role of surfactant in the pathogenesis of PHEM. METHODS: Blood samples were obtained from healthy volunteers for measuring complete blood count, PT, PTT, and platelet function...
November 2013: Thrombosis Research
A Booth, A Steel, J Klein
Major trauma is a leading cause of death and disability in the UK, particularly in the young. Pre-hospital emergency medicine (PHEM) involves provision of immediate medical care to critically ill and injured patients, across all age ranges, often in environments that may be remote and are not only physically challenging but also limited in terms of time and resources. PHEM is now a GMC-recognised subspecialty of anaesthesia or emergency medicine and the first recognised training program in the UK commenced in August 2012...
January 2013: Anaesthesia
Dimitris Platis, Nikolaos E Labrou
Plant molecular pharming is a technology that uses plants as bioreactors to produce recombinant molecules of medical and veterinary importance. In the present study, we evaluated the ability of histamine (HIM), tryptamine (TRM), phenylamine (PHEM) and tyramine (TYRM) coupled to Sepharose CL-4B via a 1,4-butanediol diglycidyl ether spacer to bind and purify human monoclonal anti-HIV antibody 2F5 (mAb 2F5) from spiked maize seed and tobacco leaf extracts. Detailed studies were carried out to determine the factors that affect the chromatographic behaviour of mAb 2F5 and also maize seed and tobacco leaf proteins...
March 2008: Journal of Separation Science
Nobuko Koshiba, Joji Ando, Xian Chen, Toshiaki Hisada
Atherosclerosis localizes at a bend andor bifurcation of an artery, and low density lipoproteins (LDL) accumulate in the intima. Hemodynamic factors are known to affect this localization and LDL accumulation, but the details of the process remain unknown. It is thought that the LDL concentration will be affected by the filtration flow, and that the velocity of this flow will be affected by deformation of the arterial wall. Thus, a coupled model of a blood flow and a deformable arterial wall with filtration flow would be invaluable for simulation of the flow field and concentration field in sequence...
June 2007: Journal of Biomechanical Engineering
A T Barnett, W Segree, A Matthews
A Pre-hospital Emergency Medical Service (PHEMS) is a vital component of a country's health service because it provides early medical care to critically ill and injured persons in the field There is evidence to show that early care reduces mortality and morbidity and offers the patient the best chance of survival and improved quality of life. Caribbean territories have been developing their PHEMS as part of a programme of health sector reform. In a study of PHEMS in 12 Caribbean countries, the Pan American Health Organization reported that there were no clear guidelines with respect to the roles and responsibilities of the physician in PHEMS in the majority of countries...
January 2006: West Indian Medical Journal
Toshihiro Nagai, Hiroaki Yokomori, Kazunori Yoshimura, Kayo Fujimaki, Masahiko Nomura, Toshifumi Hibi, Masaya Oda
The presence of microfilaments in the vicinity of sinusoidal endothelial fenestrae (SEF) suggests that the cytoskeleton of liver sinusoidal endothelial cells (LSEC) plays an important role in the modulation of SEF. In this study, we investigated actin filaments around SEF in LSECs. Monolayers of LSEC culture were established by infusing a rat liver with collagenase for 30 min and then culturing in RMPI medium for 24 h. Cells were reacted with 0.1% Triton X for 5 s and 15% glycerinated PHEM buffer (60 mM PIPES, 25 mM HEPES, 10 mM EGTA, 2 mM MgCl, pH 6...
December 2004: Medical Electron Microscopy: Official Journal of the Clinical Electron Microscopy Society of Japan
Célia M M Carnevalli, Cristina Pacheco Soares, Renato Amaro Zângaro, Antonio L B Pinheiro, Newton Soares Silva
OBJECTIVE: The present study investigated the effects of low-level laser therapy (LLLT) on the mitochondria, nucleus, and cytoskeleton of CHO K-1 cells by the use of specific fluorescent probes. BACKGROUND DATA: The use of LLLT has been recommended by several authors for acceleration of the healing process. The literature on the effects of LLLT in this process is highly contradictory because of difficulties in identifying its effects on cells. MATERIALS AND METHODS: CHO K-1 cells were cultivated using MEM containing 5% FBS and were irradiated or not with a semiconductor laser (lambda = 830 nm; phi approximately 0...
August 2003: Journal of Clinical Laser Medicine & Surgery
J G Oliveira-Lima, J R Mineo, A A Santos, G L Ferreira, E A Ferro, C A Oliveira
To evaluate an improved method for identifying the presence of the structural elements of the cytoskeleton of Toxoplasma gondii and their influence on invasion of the parasite in host cells, copper grids coated with plastic film were used for adhesion of whole parasites. Tachyzoites were incubated with 0.5% Triton X-100 in PHEM buffer containing protease inhibitors, post-fixed in 1% glutaraldehyde, stained with uranyl acetate and submitted to critical point drying. In order to analyze the presence of the structural elements of the cytoskeleton, immunolocalization was carried out using colloidal gold...
April 2001: Parasitology Research
E Leisch, M Tsiknakis, S C Orphanoudakis
In this paper, a Pre-hospital Health Emergency Management System (PHEMS) is presented, which is being developed on the basis of a common reference architecture that has been defined at a European level by partners from ten EU member states in the course of the ongoing HECTOR project. The PHEMS, which is implemented as an autonomous system, will be integrated as an added-value service into the Regional Health Telematics Network of Crete. The PHEMS architecture is based on a 'perception-cognition-action' paradigm...
1997: Studies in Health Technology and Informatics
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