keyword
MENU ▼
Read by QxMD icon Read
search

Phea

keyword
https://www.readbyqxmd.com/read/29143777/margination-of-fluorescent-polylactic-acid-polyaspartamide-based-nanoparticles-in-microcapillaries-in-vitro-the-effect-of-hematocrit-and-pressure
#1
Emanuela Fabiola Craparo, Rosa D'Apolito, Gaetano Giammona, Gennara Cavallaro, Giovanna Tomaiuolo
The last decade has seen the emergence of vascular-targeted drug delivery systems as a promising approach for the treatment of many diseases, such as cardiovascular diseases and cancer. In this field, one of the major challenges is carrier margination propensity (i.e., particle migration from blood flow to vessel walls); indeed, binding of these particles to targeted cells and tissues is only possible if there is direct carrier-wall interaction. Here, a microfluidic system mimicking the hydrodynamic conditions of human microcirculation in vitro is used to investigate the effect of red blood cells (RBCs) on a carrier margination in relation to RBC concentration (hematocrit) and pressure drop...
October 28, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/29035554/star-polymers-reduce-iapp-toxicity-via-accelerated-amyloid-aggregation
#2
Emily H Pilkington, May Lai, Xinwei Ge, William J Stanley, Bo Wang, Miaoyi Wang, Aleksandr Kakinen, Marc-Antoine Sani, Michael R Whittaker, Esteban N Gurzov, Feng Ding, John F Quinn, Thomas P Davis, Pu Chun Ke
Protein aggregation into amyloid fibrils is a ubiquitous phenomenon across the spectrum of neurodegenerative disorders and type 2 diabetes. A common strategy against amyloidogenesis is to minimize the populations of toxic oligomers and protofibrils by inhibiting protein aggregation with small molecules or nanoparticles. However, melanin synthesis in nature is realized by accelerated protein fibrillation to circumvent accumulation of toxic intermediates. Accordingly, we designed and demonstrated the use of star-shaped poly(2-hydroxyethyl acrylate) (PHEA) nanostructures for promoting the aggregation while ameliorating the toxicity of human islet amyloid polypeptide (IAPP), the peptide involved in glycemic control and the pathology of type 2 diabetes...
October 16, 2017: Biomacromolecules
https://www.readbyqxmd.com/read/28974694/phea-g-pmma-well-defined-graft-copolymer-atrp-synthesis-self-assembly-and-synchronous-encapsulation-of-both-hydrophobic-and-hydrophilic-guest-molecules
#3
Aishun Ding, Jie Xu, Guangxin Gu, Guolin Lu, Xiaoyu Huang
A series of well-defined amphiphilic graft copolymer bearing a hydrophilic poly(2-hydroxyethyl acrylate) (PHEA) backbone and hydrophobic poly(methyl methacrylate) (PMMA) side chains was synthesized by successive reversible addition-fragmentation chain transfer (RAFT) polymerization and atom transfer radical polymerization (ATRP) through the grafting-from strategy. A well-defined PHEA-based backbone with Cl-containing ATRP initiating group in every repeated unit (M w/M n = 1.08), poly(2-hydroxyethyl 2-((2-chloropropanoyloxy)methyl)acrylate) (PHECPMA), was first prepared by RAFT homopolymerization of 2-hydroxyethyl 2-((2-chloropropanoyloxy)methyl)acrylate (HECPMA), a Cl-containing trifunctional acrylate...
October 3, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28917533/nanoparticles-of-a-polyaspartamide-based-brush-copolymer-for-modified-release-of-sorafenib-in-vitro-and-in-vivo-evaluation
#4
Melchiorre Cervello, Giovanna Pitarresi, Antonella Bavuso Volpe, Barbara Porsio, Daniele Balasus, Maria Rita Emma, Antonina Azzolina, Roberto Puleio, Guido Ruggero Loria, Stefano Puleo, Gaetano Giammona
In this paper, we describe the preparation of polymeric nanoparticles (NPs) loaded with sorafenib for the treatment of hepatocellular carcinoma (HCC). A synthetic brush copolymer, named PHEA-BIB-ButMA (PBB), was synthesized by Atom Trasnfer Radical Polymerization (ATRP) starting from the α-poly(N-2-hydroxyethyl)-d,l-aspartamide (PHEA) and poly butyl methacrylate (ButMA). Empty and sorafenib loaded PBB NPs were, then, produced by using a dialysis method and showed spherical morphology, colloidal size, negative ζ potential and the ability to allow a sustained sorafenib release in physiological environment...
September 14, 2017: Journal of Controlled Release: Official Journal of the Controlled Release Society
https://www.readbyqxmd.com/read/28872702/enhancement-of%C3%A2-l-phenylalanine-production-in-escherichia-coli-by-heterologous-expression-of-vitreoscilla-hemoglobin
#5
Wei-Bin Wu, Xiao-Lei Guo, Ming-Liang Zhang, Qing-Gen Huang, Feng Qi, Jian-Zhong Huang
l-Phenylalanine is an important amino acid that is widely used in the production of food flavors and pharmaceuticals. Generally, l-phenylalanine production by engineered Escherichia coli requires a high rate of oxygen supply. However, the coexpression of Vitreoscilla hemoglobin gene (vgb), driven bya tac promoter, with the genes encoding 3-deoxy-d-arabinoheptulosonate-7-phosphate synthetase (aroF) and feedback-resistant chorismate mutase/prephenate dehydratase (pheA(fbr) ), led to increased productivity and decreased demand for aeration by E...
September 5, 2017: Biotechnology and Applied Biochemistry
https://www.readbyqxmd.com/read/28805713/polyaspartamide-based-nanoparticles-loaded-with-fluticasone-propionate-and-the-in-vitro-evaluation-towards-cigarette-smoke-effects
#6
Emanuela Fabiola Craparo, Maria Ferraro, Elisabetta Pace, Maria Luisa Bondì, Gaetano Giammona, Gennara Cavallaro
This paper describes the evaluation of polymeric nanoparticles (NPs) as a potential carrier for lung administration of fluticasone propionate (FP). The chosen polymeric material to produce NPs was a copolymer based on α,β-poly(N-2-hydroxyethyl)-d,l-aspartamide (PHEA) whose backbone was derivatised with different molecules, such as poly(lactic acid) (PLA) and polyethylenglycol (PEG). The chosen method to produce NPs from PHEA-PLA-PEG2000 was the method based on high-pressure homogenization and subsequent solvent evaporation by adding Pluronic F68 during the process and trehalose before lyophilisation...
August 13, 2017: Nanomaterials
https://www.readbyqxmd.com/read/28666356/modphea-model-organism-phenotype-enrichment-analysis-of-eukaryotic-gene-sets
#7
Meng-Pin Weng, Ben-Yang Liao
Motivation: Genome-scale phenotypic data are available for many model organisms, yet existing tools to functionally interpret gene sets from these phenotypic data are largely based on mutagenesis-derived phenotypes observed in mouse or human. Results: Data from both mutagenesis and knockdown experiments are incorporated into modPhEA to allow users to perform enrichment analyses based on phenotypes observed in budding yeast (Saccharomyces cerevisiae), roundworm (Caenorhabditis elegans), fruit fly (Drosophila melanogaster), zebrafish (Danio rerio), mouse (Mus musculus) and humans (Homo sapiens)...
November 1, 2017: Bioinformatics
https://www.readbyqxmd.com/read/28623473/increasing-the-bile-acid-sequestration-performance-of-cationic-hydrogels-by-using-an-advanced-controlled-polymerization-technique
#8
Patrícia V Mendonça, André Matos, Andreia F Sousa, Arménio C Serra, Sérgio Simões, Jorge F J Coelho
PURPOSE: To investigate the influence of the polymerization technique and the content of hydroxyl groups on the performance of new bile acid sequestrants based on PAMPMTA-co-PHEA (PAMPTMA: poly((3-acrylamidopropyl)trimethylammonium chloride); PHEA: poly(2-hydroxyethyl acrylate)) hydrogels. METHODS: PAMPMTA-co-PHEA hydrogels were prepared using either free radical polymerization or supplemental activator and reducing agent atom transfer radical polymerization. The chemical structure and composition of the hydrogels was confirmed by both FTIR and ssNMR...
September 2017: Pharmaceutical Research
https://www.readbyqxmd.com/read/28553303/phenylpyruvate-contributes-to-the-synthesis-of-fragrant-benzenoid-phenylpropanoids-in-petunia-%C3%A3-hybrida-flowers
#9
Moran Oliva, Einat Bar, Rinat Ovadia, Avichai Perl, Gad Galili, Efraim Lewinsohn, Michal Oren-Shamir
Phenylalanine (Phe) is a precursor for a large group of plant specialized metabolites, including the fragrant volatile benzenoid-phenylpropanoids (BPs). In plants, the main pathway leading to production of Phe is via arogenate, while the pathway via phenylpyruvate (PPY) is considered merely an alternative route. Unlike plants, in most microorganisms the only pathway leading to the synthesis of Phe is via PPY. Here we studied the effect of increased PPY production in petunia on the formation of BPs volatiles and other specialized metabolites originating from Phe both in flowers and leaves...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28530096/engineering-eschericha-coli-for-enhanced-tyrosol-production
#10
Yuxiang Xue, Xianzhong Chen, Cui Yang, Junzhuang Chang, Wei Shen, You Fan
Tyrosol is a phenolic compound found in olive oil and wines. The health benefits of tyrosol have attracted considerable attention. Because the tyrosol extraction from plants poses a major obstacle, biosynthesizing this compound using microbial hosts is of interest. In this study, the phenylpyruvate decarboxylase gene ARO10 and the aromatic amino acid aminotransferase gene ARO8 were introduced into Escherichia coli to generate two recombinant tyrosol producers. Deleting the prephenate dehydratase gene pheA and the phenylacetaldehyde dehydrogenase gene feaB improved the tyrosol production...
June 14, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28511681/chromosome-engineering-of-escherichia-coli-for-constitutive-production-of-salvianic-acid-a
#11
Liang Zhou, Qi Ding, Guo-Zhen Jiang, Zhen-Ning Liu, Hai-Yan Wang, Guang-Rong Zhao
BACKGROUND: Salvianic acid A (SAA), a valuable natural product from herbal plant Salvia miltiorrhiza, exhibits excellent antioxidant activities on food industries and efficacious therapeutic potential on cardiovascular diseases. Recently, production of SAA in engineered Escherichia coli was established via the artificial biosynthetic pathway of SAA on the multiple plasmids in our previous work. However, the plasmid-mediated system required to supplement expensive inducers and antibiotics during the fermentation process, restricting scale-up production of SAA...
May 16, 2017: Microbial Cell Factories
https://www.readbyqxmd.com/read/28482592/photocrosslinkable-polyaspartamide-polylactide-copolymer-and-its-porous-scaffolds-for-chondrocytes
#12
Calogero Fiorica, Fabio Salvatore Palumbo, Giovanna Pitarresi, Gaetano Giammona
With the aim to produce, by a simple and reproducible technique, porous scaffolds potentially employable for tissue engineering purposes, in this work, we have synthesized a methacrylate (MA) copolymer of α,β-poly(N-2-hydroxyethyl)-dl-aspartamide (PHEA) and polylactic acid (PLA). PHEA-PLA-MA has been dissolved in organic solvent at different concentrations in the presence of NaCl particles with different granulometry, and through UV irradiation and further salt leaching technique, various porous scaffolds have been prepared...
July 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28457379/electrospun-phea-pla-pcl-scaffold-for-vascular-regeneration-a-preliminary-in%C3%A2-vivo-evaluation
#13
S Buscemi, V D Palumbo, A Maffongelli, S Fazzotta, F S Palumbo, M Licciardi, C Fiorica, R Puleio, G Cassata, L Fiorello, G Buscemi, A I Lo Monte
BACKGROUND: There is increasing interest in the development of vessel substitutes, and many studies are currently focusing on the development of biodegradable scaffolds capable of fostering vascular regeneration. We tested a new biocompatible and biodegradable material with mechanical properties similar to those of blood vessels. METHODS: The material used comprises a mixture of α,β-poly(N-2-hydroxyethyl)-d,l-aspartamide (PHEA) and polylactic acid (PLA), combined with polycaprolactone (PCL) by means of electrospinning technique...
May 2017: Transplantation Proceedings
https://www.readbyqxmd.com/read/28457378/electrospun-polyhydroxyethyl-aspartamide-polylactic-acid-scaffold-for-biliary-duct-repair-a-preliminary-in%C3%A2-vivo-evaluation
#14
S Buscemi, G Damiano, S Fazzotta, A Maffongelli, V D Palumbo, S Ficarella, C Fiorica, G Cassata, M Licciardi, F S Palumbo, L Gulotta, G Buscemi, A I Lo Monte
Tissue engineering has emerged as a new approach with the potential to overcome the limitations of traditional therapies. The objective of this study was to test whether our polymeric scaffold is able to resist the corrosive action of bile and to support a cell's infiltration and neoangiogenesis with the aim of using it as a biodegradable tissue substitute for serious bile duct injuries. In particular, a resorbable electrospun polyhydroxyethyl-aspartamide-polylactic acid (90 mol% PHEA, 10 mol% PLA)/polycaprolactone (50:50 w/w) plate scaffold was implanted into rabbit gallbladder to assess the in vivo effects of the lytic action of the bile on the scaffold structure and then as a tubular scaffold to create a biliary-digestive anastomosis as well...
May 2017: Transplantation Proceedings
https://www.readbyqxmd.com/read/28416401/polymeric-nanoparticles-for-sirna-delivery-production-and-applications
#15
Gennara Cavallaro, Carla Sardo, Emanuela Fabiola Craparo, Barbara Porsio, Gaetano Giammona
Gene therapy through the use of siRNA and a polymeric carrier are becoming an efficient therapeutic option to conventional pharmaceutical formulations for the treatment of deadly diseases, such as cancer, pulmonary, ocular and neurodegenerative diseases. However, several considerations regarding the stability, formulation, and efficacy have to be faced up until these systems could be considered to be a marketable pharmaceutical products for to extend siRNA application to clinical practice. This review is focused on the key challenges of siRNA therapeutics, with special attention on the faced obstacles and on the formulation-related difficulties, providing a list of requirements needed for obtaining an ideal carrier for siRNA...
April 14, 2017: International Journal of Pharmaceutics
https://www.readbyqxmd.com/read/28397883/chemical-and-mechanical-modulation-of-polymeric-micelle-assembly
#16
Nicholas E Clay, Joseph J Whittenberg, Jiayu Leong, Vivek Kumar, Jinrong Chen, Insil Choi, Evangelos Liamas, Jeremy M Schieferstein, Jae Hyun Jeong, Dong Hyun Kim, Zhenyu Jason Zhang, Paul J A Kenis, Il Won Kim, Hyunjoon Kong
Recently, polymeric micelles self-assembled from amphiphilic polymers have been studied for various industrial and biomedical applications. This nanoparticle self-assembly typically occurs in a solvent-exchange process. In this process, the quality of the resulting particles is uncontrollably mediated by polymeric solubility and mixing conditions. Here, we hypothesized that improving the solubility of an amphiphilic polymer in an organic solvent via chemical modification while controlling the mixing rate of organic and aqueous phases would enhance control over particle morphology and size...
April 20, 2017: Nanoscale
https://www.readbyqxmd.com/read/28383273/near-infrared-light-responsive-folate-targeted-gold-nanorods-for-combined-photothermal-chemotherapy-of-osteosarcoma
#17
Anna Li Volsi, Cinzia Scialabba, Valeria Vetri, Gennara Cavallaro, Mariano Licciardi, Gaetano Giammona
Folate-targeted gold nanorods (GNRs) are proposed as selective theranostic agents for osteosarcoma treatment. An amphiphilic polysaccharide based graft-copolymer (INU-LA-PEG-FA) and an amino derivative of the α,β-poly(N-2-hydroxyethyl)-d,l-aspartamide functionalized with folic acid (PHEA-EDA-FA), have been synthesized to act as coating agents for GNRs. The obtained polymer-coated GNRs were characterized in terms of size, shape, zeta potential, chemical composition, and aqueous stability. They protected the anticancer drug nutlin-3 and were able to deliver it efficiently in different physiological media...
April 14, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28119125/galactosylated-polyaspartamide-copolymers-for-sirna-targeted-delivery-to-hepatocellular-carcinoma-cells
#18
Gennara Cavallaro, Rossella Farra, Emanuela Fabiola Craparo, Carla Sardo, Barbara Porsio, Gaetano Giammona, Francesca Perrone, Mario Grassi, Gabriele Pozzato, Gabriele Grassi, Barbara Dapas
The limited efficacy of available treatments for hepatocellular carcinoma (HCC) requires the development of novel therapeutic approaches. We synthesized a novel cationic polymer based on α,β-poly-(N-2-hydroxyethyl)-d,L-aspartamide (PHEA) for drug delivery to HCC cells. The copolymer was synthesized by subsequent derivatization of PHEA with diethylene triamine (DETA) and with a polyethylene glycol (PEG) derivative bearing galactose (GAL) molecules, obtaining the cationic derivative PHEA-DETA-PEG-GAL. PHEA-DETA-PEG-GAL has suitable chemical-physical characteristics for a potential systemic use and can effectively deliver a siRNA (siE2F1) targeted against the transcription factor E2F1, a gene product involved in HCC...
January 21, 2017: International Journal of Pharmaceutics
https://www.readbyqxmd.com/read/28093325/gold-nanostar-polymer-hybrids-for-sirna-delivery-polymer-design-towards-colloidal-stability-and-in-vitro-studies-on-breast-cancer-cells
#19
Carla Sardo, Barbara Bassi, Emanuela F Craparo, Cinzia Scialabba, Elisa Cabrini, Giacomo Dacarro, Agnese D'Agostino, Angelo Taglietti, Gaetano Giammona, Piersandro Pallavicini, Gennara Cavallaro
To overcome the low bioavailability of siRNA (small interfering RNA) and to improve their transfection efficiency, the use of non-viral delivery carriers is today a feasible approach to transform the discovery of these incredibly potent and versatile drugs into clinical practice. Polymer-modified gold nanoconstructs (AuNCs) are currently viewed as efficient and safe intracellular delivery carriers for siRNA, as they have the possibility to conjugate the ability to stably entrap and deliver siRNAs inside cells with the advantages of gold nanoparticles, which can act as theranostic agents and radiotherapy enhancers through laser-induced hyperthermia...
March 15, 2017: International Journal of Pharmaceutics
https://www.readbyqxmd.com/read/28049530/metabolic-engineering-of-indole-pyruvic-acid-biosynthesis-in-escherichia-coli-with-tdid
#20
Yelin Zhu, Yan Hua, Biao Zhang, Lianhong Sun, Wenjie Li, Xin Kong, Jiong Hong
BACKGROUND: Indole pyruvic acid (IPA) is a versatile platform intermediate and building block for a number of high-value products in the pharmaceutical and food industries. It also has a wide range of applications, such as drugs for the nervous system, cosmetics, and luminophores. Chemical synthesis of IPA is a complicated and costly process. Moreover, through the biosynthesis route employing L-amino acid oxidase, the byproduct hydrogen peroxide leads the degradation of IPA. TdiD, identified as a specific tryptophan aminotransferase, could be an alternative solution for efficient IPA biosynthesis...
January 3, 2017: Microbial Cell Factories
keyword
keyword
37195
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"