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https://www.readbyqxmd.com/read/28087105/left-ventricular-assist-device-outcomes-based-on-flow-configuration-and-pre-operative-left-ventricular-dimension-an-interagency-registry-for-mechanically-assisted-circulatory-support-analysis
#1
Palak Shah, Sarah Birk, Simon Maltais, John Stulak, Angelo Elmi, Francis D Pagani, Jennifer A Cowger
BACKGROUND: Axial configuration (AC) and centrifugal configuration (CC) left ventricular assist devices (LVAD) have different flow characteristics, and whether the interaction between device flow configuration and the pre-operative left ventricular internal diastolic diameter (LVIDD) mediates adverse events after LVAD implantation is unknown. METHODS: We queried 9,424 continuous-flow LVAD recipients who received LVADs from April 2008 to June of 2015 in the Interagency Registry for Mechanically Assisted Circulatory Support (INTERMACS)...
December 16, 2016: Journal of Heart and Lung Transplantation
https://www.readbyqxmd.com/read/28081264/the-important-role-of-lipid-raft-mediated-attachment-in-the-infection-of-cultured-cells-by-coronavirus-infectious-bronchitis-virus-beaudette-strain
#2
Huichen Guo, Mei Huang, Quan Yuan, Yanquan Wei, Yuan Gao, Lejiao Mao, Lingjun Gu, Yong Wah Tan, Yanxin Zhong, Dingxiang Liu, Shiqi Sun
Lipid raft is an important element for the cellular entry of some viruses, including coronavirus infectious bronchitis virus (IBV). However, the exact role of lipid rafts in the cellular membrane during the entry of IBV into host cells is still unknown. In this study, we biochemically fractionated IBV-infected cells via sucrose density gradient centrifugation after depleting plasma membrane cholesterol with methyl-β-cyclodextrin or Mevastatin. Our results demonstrated that unlike IBV non-structural proteins, IBV structural proteins co-localized with lipid raft marker caveolin-1...
2017: PloS One
https://www.readbyqxmd.com/read/28080032/heteroprotein-complex-formation-of-bovine-lactoferrin-and-pea-protein-isolate-a-multiscale-structural-analysis
#3
Eda Adal, Amin Sadeghpour, Simon D A Connell, Michael Rappolt, Esra Ibanoglu, Anwesha Sarkar
Associative electrostatic interactions between two oppositely charged globular proteins, lactoferrin (LF) and pea protein isolate (PPI), the latter being a mixture of vicilin, legumin and convicilin, was studied with a specific PPI/ LF molar ratio at room temperature. Structural aspects of the electrostatic complexes probed at different length scales were investigated as a function of pH by means of different complementary techniques, namely with dynamic light scattering, small angle X-ray scattering (SAXS), turbidity measurements and atomic force microscopy (AFM)...
January 12, 2017: Biomacromolecules
https://www.readbyqxmd.com/read/28078889/quantification-of-free-polyelectrolytes-present-in-colloidal-suspension-revealing-a-source-of-toxic-responses-for-polyelectrolyte-wrapped-gold-nanoparticles
#4
Tian A Qiu, Marco D Torelli, Ariane M Vartanian, Nathan B Rackstraw, Joseph T Buchman, Lisa M Jacob, Catherine J Murphy, Robert J Hamers, Christy L Haynes
Polyelectrolyte (PE) wrapping of colloidal nanoparticles (NPs) is a standard method to control NP surface chemistry and charge. Because excess polyelectrolytes are usually employed in the surface modification process, it is critical to evaluate different purification strategies to obtain a clean final product and thus avoid ambiguities in the source of effects on biological systems. In this work, 4 nm diameter gold nanoparticles (AuNPs) were wrapped with 15 kDa poly(allylamine hydrochloride) (PAH), and three purification strategies were applied: (a) diafiltration or either (b) one round or (c) two rounds of centrifugation...
January 10, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28078589/isolation-of-lipid-rafts-through-discontinuous-sucrose-gradient-centrifugation-and-fas-cd95-death-receptor-localization-in-raft-fractions
#5
Consuelo Gajate, Faustino Mollinedo
Lipid raft domains, enriched in sphingolipids and cholesterol, serve as sorting platforms and hubs for signal transduction proteins, and show resistance to detergent solubilization. Despite rafts have been involved in survival processes, these membrane domains have also been shown to play a major role in the modulation of death receptor signaling. Here, we describe a detailed protocol for isolating lipid rafts from whole cells by taking advantage of the lipid raft resistance to Triton X-100 solubilization at 4 °C, followed by sucrose gradient centrifugation, with subsequent analysis of Fas/CD95 death receptor localization in the raft fractions by immunoblotting...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28078587/organelle-separation-and-cell-signaling
#6
Leandro Silva Da Costa, Damien Arnoult
Recent findings indicate that some signaling hubs coalesce at the surfaces of organelles through the accumulation of ubiquitylated components required for the signal transduction. For instance, ubiquitylated components of the NF-κB pathway accumulated at the endoplasmic reticulum while ubiquitylated components of the IRF3 pathway are found at the Golgi apparatus. Here we describe simple methods to observe and assess these ubiquitylated components by immunoblotting using differential centrifugation and in vitro assays...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28078525/chitosan-ceramide-coating-on-gold-nanorod-to-improve-its-physiological-stability-and-reduce-the-lipid-surface-related-toxicity
#7
Gantumur Battogtokh, Oyuntuya Gotov, Young Tag Ko
Gold nanoparticles are promising materials for many applications that include imaging, drug delivery, and photothermal therapy. However, AuNPs can be unstable and/or toxic. We purposed to improve the stability and reduce toxicity of gold nanorods (AuNR) upon coating with biocompatible polymer, chitosan-ceramide (CS-CE), without replacing the original layer, CTAB. CS-CE-coated AuNR was prepared by simple mixing for 24 h and purified by centrifugation. The coating was confirmed by UV-Vis absorption analysis and surface charge and size measurement...
January 11, 2017: Archives of Pharmacal Research
https://www.readbyqxmd.com/read/28076980/status-of-sperm-born-oocyte-activating-factors-pawp-plc%C3%AE-and-sperm-chromatin-in-uncapacitated-capacitated-and-acrosome-reacted-conditions
#8
Marziyeh Tavalaee, Kazem Parivar, Abdol-Hossein Shahverdi, Kamran Ghaedi, Mohammad Hossein Nasr-Esfahani
The aim of this study was to investigate the value of sperm factors PAWP and PLCζ involved in oocyte activation and sperm chromatin status, as indicators of sperm quality during uncapacitated, capacitated and acrosome-reacted conditions. Semen samples collected from 10 normozoospermic men and sperm factors PAWP, PLCζ, protamine deficiency and DNA fragmentation evaluated in fresh samples, processed sample by DGC (density gradient centrifugation), capacitated and acrosome reacted sperm, using immunofluorescence and flow cytometry...
January 12, 2017: Human Fertility: Journal of the British Fertility Society
https://www.readbyqxmd.com/read/28076459/evaluation-of-phenyl-propanedione-on-yellowing-and-chemical-mechanical-properties-of-experimental-dental-resin-based-materials
#9
Dayane Carvalho Ramos Salles de Oliveira, Eduardo José Souza-Junior, Adam Dobson, Ana Rosa Costa Correr, William Cunha Brandt, Mário Alexandre Coelho Sinhoreti
Objective: To evaluate the influence of phenyl-propanedione on yellowing and chemical-mechanical properties of experimental resin-based materials photoactivated using different light curing units (LCUs). Material and Methods: Experimental resin-based materials with the same organic matrix (60:40 wt% BisGMA:TEGDMA) were mechanically blended using a centrifugal mixing device. To this blend, different photoinitiator systems were added in equimolar concentrations with aliphatic amine doubled by wt%: 0...
November 2016: Journal of Applied Oral Science: Revista FOB
https://www.readbyqxmd.com/read/28075595/adsorption-of-small-cationic-nanoparticles-onto-large-anionic-particles-from-aqueous-solution-a-model-system-for-understanding-pigment-dispersion-and-the-problem-of-effective-particle-density
#10
Shannon M North, Elizabeth R Jones, Gregory N Smith, Oleksandr O Mykhaylyk, Tom Annable, Steven P Armes
This work focuses on the use of copolymer nanoparticles as a dispersant for a model pigment (silica). Reversible addition-fragmentation chain transfer (RAFT) alcoholic dispersion polymerization was used to synthesize sterically stabilized diblock copolymer nanoparticles. The steric stabilizer block was poly(2-dimethylamino)ethyl methacrylate) [PDMA] while the core-forming block was poly(benzyl methacrylate) [PBzMA]. The mean degrees of polymerization for the PDMA and PBzMA blocks were 71 and 100, respectively...
January 11, 2017: Langmuir: the ACS Journal of Surfaces and Colloids
https://www.readbyqxmd.com/read/28074943/the-influence-of-flaxseed-gum-on-the-microrheological-properties-and-physicochemical-stability-of-whey-protein-stabilized-%C3%AE-carotene-emulsions
#11
Duoxia Xu, Yameng Qi, Xu Wang, Xin Li, Shaojia Wang, Yanping Cao, Chengtao Wang, Baoguo Sun, Eric Decker, Atikorn Panya
The impact of flaxseed gum (FG) on the microrheological properties and physicochemical stability of whey protein isolate (WPI) stabilized β-carotene emulsions at pH 3.0 was studied. A layer-by-layer electrostatic deposition method was used to prepare multilayered β-carotene emulsions with interfacial membranes consisting of WPI and FG. The microrheological behavior of the multilayered β-carotene emulsions was measured through the diffusive wave spectroscopy technique. WPI alone and WPI-FG (concentration of FG = 0...
January 11, 2017: Food & Function
https://www.readbyqxmd.com/read/28074941/enhancing-the-detection-of-dysmorphic-red-blood-cells-and-renal-tubular-epithelial-cells-with-a-modified-urinalysis-protocol
#12
Yu Chu-Su, Kenichi Shukuya, Takashi Yokoyama, Wei-Chou Lin, Chih-Kang Chiang, Chii-Wann Lin
Urinary sediment is used to evaluate patients with possible urinary tract diseases. Currently, numerous protocols are applied to detect dysmorphic red blood cells (RBCs) and renal tubular epithelial cells (RTECs) in urinary sediment. However, distinct protocols are used by nephrologists and medical technologists for specimen concentration and observation, which leads to major discrepancies in the differential counts of formed elements such as dysmorphic RBCs and RTECs and might interfere with an accurate clinical diagnosis...
January 11, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28073193/analysis-of-myo-inositol-effect-on-spermatozoa-motility-in-hyper-viscous-ejaculates-and-in-patients-with-grades-ii-and-iii-varicocele
#13
Filomena Scarselli, Anna Maria Lobascio, Mario Terribile, Valentina Casciani, Pierfrancesco Greco, Giorgio Franco, Maria Giulia Minasi, Ermanno Greco
The goal of this study is to evaluate MYOInositol effects on spermatozoa motility, in patients' ejaculates with severe varicocele or hyper viscosity. The study included normal viscosity ejaculate from 30 patients affected by varicocele and hyper viscosity ejaculate from 33 patients without any testicular pathologies. All selected samples showed sperm concentration > 2 million/ml and progressive motility < 32%. In both groups, the pellet obtained after centrifugation in buffered medium, was divided in two aliquots, both incubated for 15 minutes at 37°C: one with MYO-Inositol and the other one, as control, only in phosphate buffered saline (PBS)...
December 30, 2016: Archivio Italiano di Urologia, Andrologia
https://www.readbyqxmd.com/read/28071001/purified-isolation-of-vacuoles-from-sedum-alfredii-leaf-derived-protoplasts
#14
Xiao-Yu Gao, Xing-Cheng Liao, Ruo-Lai Wu, Ting Liu, Hai-Xing Wang, Ling-Li Lu
This study aims to develop a method for isolating and purifying protoplasts/vacuoles from fresh leaves of the Cd hyperaccumulator plant species, Sedum alfredii. The results revealed that preheating cellulase and macerozyme at 50 °C for 5 min significantly accelerated the cell wall degradation. For the most optimal conditions for mesophyll protoplast isolation, the mixture of fresh leaves and cell lysates was followed by a 2-h-long vibration. The protoplast lysate for vacuole isolation was diluted, and 0.675 mmol/L was identified as the most appropriate 3-[(3-cholamidopropyl) dimethylammonio]-1-propanesulfonic acid (CHAPS) level, in which S...
2017: Journal of Zhejiang University. Science. B
https://www.readbyqxmd.com/read/28070222/scale-up-and-process-integration-of-sugar-production-by-acidolysis-of-municipal-solid-waste-corn-stover-blends-in-ionic-liquids
#15
Chenlin Li, Ling Liang, Ning Sun, Vicki S Thompson, Feng Xu, Akash Narani, Qian He, Deepti Tanjore, Todd R Pray, Blake A Simmons, Seema Singh
BACKGROUND: Lignocellulosic biorefineries have tonnage and throughput requirements that must be met year round and there is no single feedstock available in any given region that is capable of meeting the price and availability demands of the biorefineries scheduled for deployment. Significant attention has been historically given to agriculturally derived feedstocks; however, a diverse range of wastes, including municipal solid wastes (MSW), also have the potential to serve as feedstocks for the production of advanced biofuels and have not been extensively studied...
2017: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/28068467/exodisc-for-rapid-size-selective-and-efficient-isolation-and-analysis-of-nanoscale-extracellular-vesicles-from-biological-samples
#16
Hyun-Kyung Woo, Vijaya Sunkara, Juhee Park, Tae-Hyeong Kim, Ja-Ryoung Han, Chi-Ju Kim, Hyun-Il Choi, Yoon-Keun Kim, Yoon-Kyoung Cho
Extracellular vesicles (EVs) are cell-derived, nanoscale vesicles that carry nucleic acids and proteins from their cells of origin and show great potential as biomarkers for many diseases, including cancer. Efficient isolation and detection methods are prerequisites for exploiting their use in clinical settings and understanding their physiological functions. Here, we presented a rapid, label-free, and highly sensitive method for EV isolation and quantification using a lab-on-a-disc integrated with two nanofilters (Exodisc)...
January 9, 2017: ACS Nano
https://www.readbyqxmd.com/read/28064225/influence-of-mechanical-force-on-bone-matrix-proteins-in-ovariectomised-mice-and-osteoblast-like-mc3t3-e1-cells
#17
Meng Zhang, Shintaro Ishikawa, Tomoko Inagawa, Hideshi Ikemoto, Shiyu Guo, Masataka Sunagawa, Tadashi Hisamitsu
AIM: To investigate the effect of mechanical stress on periostin and semaphorin-3A expression in a murine model of postmenopausal osteoporosis and in osteoblast-like MC3T3-E1 cells. MATERIALS AND METHODS: Female mice were divided into three groups and treated with a sham operation, ovariectomy (OVX) or OVX plus treadmill training (OVX+Run). After 10 weeks, tibias were used for histological analysis. MC3T3-E1 cells were burdened by mechanical stress using a centrifuge or were treated with periostin, and the production of biologically-active semaphorin-3A was examined in vitro...
2, 2017: In Vivo
https://www.readbyqxmd.com/read/28063146/pretreatment-optimization-of-the-biomass-of-microcystis-aeruginosa-for-efficient-bioethanol-production
#18
Muhammad Imran Khan, Moon Geon Lee, Jin Hyuk Shin, Jong Deog Kim
Microalgae are considered to be the future promising sources of biofuels and bio products. The algal carbohydrates can be fermented to bioethanol after pretreatment process. Efficient pretreatment of the biomass is one of the major requirements for commercialization of the algal based biofuels. In present study the microalga, M. aeruginsa was used for pretreatment optimization and bioethanol production. Treatment of algal biomass with CaO before acid and/or enzymatic hydrolysis enhanced the degradation of algal cells...
December 2017: AMB Express
https://www.readbyqxmd.com/read/28060313/generation-of-human-monocyte-derived-dendritic-cells-from-whole-blood
#19
Wilfried Posch, Cornelia Lass-Flörl, Doris Wilflingseder
Dendritic cells (DCs) recognize foreign structures of different pathogens, such as viruses, bacteria, and fungi, via a variety of pattern recognition receptors (PRRs) expressed on their cell surface and thereby activate and regulate immunity. The major function of DCs is the induction of adaptive immunity in the lymph nodes by presenting antigens via MHC I and MHC II molecules to naïve T lymphocytes. Therefore, DCs have to migrate from the periphery to the lymph nodes after the recognition of pathogens at the sites of infection...
December 24, 2016: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/28060312/preparation-of-liquid-exfoliated-transition-metal-dichalcogenide-nanosheets-with-controlled-size-and-thickness-a-state-of-the-art-protocol
#20
Claudia Backes, Damien Hanlon, Beata M Szydlowska, Andrew Harvey, Ronan J Smith, Thomas M Higgins, Jonathan N Coleman
We summarize recent advances in the production of liquid-exfoliated transition metal dichalcogenide (TMD) nanosheets with controlled size and thickness. Layered crystals of molybdenum disulphide (MoS2) and tungsten disulphide (WS2) are exfoliated in aqueous surfactant solution by sonication. This yields highly polydisperse mixtures containing nanosheets with broad size and thickness distributions. However, for most purposes, specific sizes (in terms of both lateral dimension and thickness) are required. For example, large and thin nanosheets are desired for (opto) electronic applications, while laterally small nanosheets are interesting for catalytic applications...
December 20, 2016: Journal of Visualized Experiments: JoVE
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