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https://www.readbyqxmd.com/read/28938635/distribution-of-circulating-tumor-dna-in-lung-cancer-analysis-of-the-primary-lung-and-bone-marrow-along-with-the-pulmonary-venous-and-peripheral-blood
#1
Taichiro Goto, Yosuke Hirotsu, Kenji Amemiya, Takahiro Nakagomi, Daichi Shikata, Yujiro Yokoyama, Kenichiro Okimoto, Toshio Oyama, Hitoshi Mochizuki, Masao Omata
Circulating tumor DNA (ctDNA), extracted from plasma, is a non-invasive surrogate biomarker. However, the distribution of ctDNA in the body still remains to be elucidated. In this study, resected lung tumors, with simultaneous blood and bone marrow samples, were analyzed to elucidate the distribution of ctDNA. Rib bone marrow, pulmonary venous blood (Pul.V) and peripheral blood (Peri.B) were obtained from 30 patients. The liquid samples were divided into cell pellets and supernatant by centrifugation; a total of 212 DNA samples were subjected to massively parallel sequencing...
August 29, 2017: Oncotarget
https://www.readbyqxmd.com/read/28938327/adipose-tissue-derived-stromal-vascular-fraction-increases-osteogenesis-in-an-experimental-design-zygomatic-bone-defect-model
#2
Gaye Toplu, Derya Ozcelik, Merdan Serin, Havva Erdem, Arzu Turkseven Topacoglu
INTRODUCTION: Facial bone defects are frequently encountered problems in clinical practice. Bone grafts, flaps, and alloplastic materials are often used in their treatment. This leads to donor site morbidity and prolongation of the operation. The authors have planned this study to examine whether adipose tissue derived stromal vascular fraction (SVF) has an osteogenic effect in the critical sized membranous bone defect of the zygomatic bone. MATERIALS AND METHODS: Twenty male Wistar Albino rats were used...
September 21, 2017: Journal of Craniofacial Surgery
https://www.readbyqxmd.com/read/28938308/sensor-based-physiologic-control-strategy-for-biventricular-support-with-rotary-blood-pumps
#3
Yu Wang, Steven C Koenig, Zhongjun Wu, Mark S Slaughter, Guruprasad A Giridharan
Rotary biventricular assist devices (BiVAD) are becoming a clinically accepted treatment option for end-stage biventricular failure. To improve BiVAD efficacy and safety, we propose a control algorithm to achieve the clinical objectives of maintaining left-right-sided balance, restoring physiologic flows, and preventing ventricular suction. The control algorithm consists of two proportional-integral (PI) controllers for left and right ventricular assist devices (LVAD and RVAD) to maintain differential pump pressure across LVAD (ΔPL) and RVAD (ΔPR) to provide left-right balance and physiologic flow...
September 21, 2017: ASAIO Journal: a Peer-reviewed Journal of the American Society for Artificial Internal Organs
https://www.readbyqxmd.com/read/28937596/synthesis-of-multi-walled-carbon-nanotubes-from-plastic-waste-using-a-stainless-steel-cvd-reactor-as-catalyst
#4
Pranav K Tripathi, Shane Durbach, Neil J Coville
The disposal of non-biodegradable plastic waste without further upgrading/downgrading is not environmentally acceptable and many methods to overcome the problem have been proposed. Herein we indicate a simple method to make high-value nanomaterials from plastic waste as a partial solution to the environmental problem. Laboratory-based waste centrifuge tubes made of polypropylene were chosen as a carbon source to show the process principle. In the process, multi-walled carbon nanotubes (MWCNTs) were synthesized from plastic waste in a two-stage stainless steel 316 (SS 316) metal tube that acted as both reactor vessel and catalyst...
September 22, 2017: Nanomaterials
https://www.readbyqxmd.com/read/28936661/isolation-of-rice-sperm-cells-for-transcriptional-profiling
#5
Scott D Russell, Daniel S Jones, Sarah Anderson, Xinkun Wang, Venkatesan Sundaresan, Xiaoping Gou
The male germline of flowering plants displays unexpectedly divergent transcriptional profiles compared to other cell types and tissues of plants. As these are among the smallest cells, and are harbored within pollen, isolating a pure collection of germline RNA presents unusual challenges. The sperm cells of rice represent a particularly challenging subject for study as the pollen are unusually short lived upon release from the anther, and the marker gene sequences that make FACS possible in Arabidopsis have not yet been introduced into rice...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28935373/human-alpha-a-crystallin-missing-n-terminal-domain-poorly-complexes-with-filensin-and-phakinin
#6
Jose M Chaves, Ratna Gupta, Kiran Srivastava, Om Srivastava
The aim of this study was to determine relative importance of N-terminal domain and C-terminal extension of αA-crystallin during their in vitro complex formation with phakinin and filensin (the two lens-specific intermediate filament [IF] proteins). Cloned phakinin, filensin and vimentin were purified under a denaturing conditions by consecutive DEAE-cellulose-, hydroxyapatite- and Sephadex G-75-column chromatographic methods. WTαA-crystallin, αA-NT (N-terminal domain [residue number 1-63])-deleted and αA-CT (C-terminal terminal extension [residue number 140-173]-deleted), were cloned in pET 100 TOPO vector, expressed in BL-21 (DE3) cells using 1% IPTG, and purified using a Ni(2+)-affinity column...
September 18, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28935010/choline-chloride-oxalic-acid-as-a-deep-eutectic-solvent-based-innovative-digestion-method-for-the-determination-of-selenium-and-arsenic-in-fish-samples
#7
Abdul Haleem Panhwar, Mustafa Tuzen, Tasneem Gul Kazi
An innovative and effective digestion method based on choline chloride (ChCl)–oxalic acid (Ox) deepeutectic solvent (DES) was proposed for the determination of Se and As in fish samples via electrothermal atomic absorption spectrometry (ETAAS). The impacts of different variables, including the composition and volume of ChCl–Ox, temperature, and acid addition, on analyte recovery were studied for optimization. In this procedure, an 80 mg sample was dissolved in a 1:2 molar ratio of ChCl–Ox at 105°C for 40 min, with the subsequent addition of 4...
September 21, 2017: Journal of AOAC International
https://www.readbyqxmd.com/read/28934999/vortex-assisted-modified-dispersive-liquid-liquid-microextraction-of-trace-levels-of-cadmium-in-surface-water-and-groundwater-samples-of-tharparkar-pakistan-optimized-by-multivariate-technique
#8
Sooraj Nizamani, Tasneem G Kazi, Hassan I Afridi, Sehrish Talpur, Ayaz Lashari, Anjum Lashari, Jamshed Ali
A simple vortex-assisted modified dispersive liquid–liquid microextraction procedure is proposed forthe enrichment of cadmium (Cd(+2)) in surface (stored rainwater) and groundwater of the Tharparkar district in Pakistan, before analysis by flame atomic absorption spectrometry. Ammonium pyrrolidinedithiocarbamate was used as a ligand to make a hydrophobic complex of Cd(+2), which was extracted in an ionic liquid (1-butyl-3-methylimidazolium hexafluorophosphate), and the nonionic surfactant Triton X-114 was applied as a dispersing medium...
September 21, 2017: Journal of AOAC International
https://www.readbyqxmd.com/read/28934465/regulation-of-chromatin-folding-by-conformational-variations-of-nucleosome-linker-dna
#9
Jenna M Buckwalter, Davood Norouzi, Anna Harutyunyan, Victor B Zhurkin, Sergei A Grigoryev
Linker DNA conformational variability has been proposed to direct nucleosome array folding into more or less compact chromatin fibers but direct experimental evidence for such models are lacking. Here, we tested this hypothesis by designing nucleosome arrays with A-tracts at specific locations in the nucleosome linkers to induce inward (AT-IN) and outward (AT-OUT) bending of the linker DNA. Using electron microscopy and analytical centrifugation techniques, we observed spontaneous folding of AT-IN nucleosome arrays into highly compact structures, comparable to those induced by linker histone H1...
September 19, 2017: Nucleic Acids Research
https://www.readbyqxmd.com/read/28934218/prevalence-of-intestinal-parasites-among-inmates-in-midwest-brazil
#10
Larissa Gabrielle Curval, Adriana de Oliveira França, Henrique Jorge Fernandes, Rinaldo Pôncio Mendes, Lídia Raquel de Carvalho, Minoru German Higa, Eduardo de Castro Ferreira, Maria Elizabeth Cavalheiros Dorval
BACKGROUND: Intestinal parasitic infections constitute a public health issue in developing countries, with prevalence rates as high as 90%, a figure set to escalate as the socioeconomic status of affected populations deteriorates. Investigating the occurrence of these infections among inmates is critical, since this group is more vulnerable to the spread of a number of infectious illnesses. METHODS: This cross-sectional, analytical, quantitative study was conducted in July 2015 at prison facilities located in Midwest Brazil to estimate the prevalence of parasitic infection among inmates...
2017: PloS One
https://www.readbyqxmd.com/read/28934031/simultaneous-detection-of-15-antibiotic-growth-promoters-in-bovine-muscle%C3%AF-blood-and-urine-by-uplc-ms-ms
#11
Zhi Wang, Zongwei Shi, Cunxian Xi, Guomin Wang, Shurui Cao, Lei Zhang, Bobin Tang, Zhaode Mu
An analytical method was established for the rapid detection of antibiotic growth promoters (AGPs) in bovine muscle, and bovine blood and bovine urine, using ultra high performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS). After the addition of an aqueous solution of EDTA-Na2, the pH of bovine urine samples was directly adjusted to 5.2 by acetic acid-ammonium acetate and purified by HLB solid-phase extraction cartridge; bovine muscle and bovine blood samples processing were extracted with acetonitrile (ACN) and ACN-water (90:10; v/v) without any purification step...
September 21, 2017: Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment
https://www.readbyqxmd.com/read/28933146/self-assembled-ti-4-biospore-microspheres-for-sensitive-dna-analysis
#12
RuiHua Fei, Chen Tan, Yue Huang, Huanchun Chen, Aizhen Guo, Hailin Wang, Yonggang Hu
Ti(4+) can be chemically adsorbed and assembled on the surface of the modified spore to form highly monodispersed Ti(4+)@spore microspheres. Moreover, we for the first time found that these bio-microspheres exhibit differential affinities toward ssDNA and dsDNA. As a principle-of-proof, we exploited the self-assembled Ti(4+)@spore microspheres for a hybridization analysis. Interestingly, in hybridization analysis, residual ssDNA probes are selectively adsorbed on Ti(4+)@spore microspheres at pH 5.0 and then removed via centrifugation...
September 21, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28931396/il-4-induced-caveolin-1-containing-lipid-rafts-aggregation-contributes-to-muc5ac-synthesis-in-bronchial-epithelial-cells
#13
Yu Xia, Peng-Cheng Cai, Fan Yu, Liang Xiong, Xin-Liang He, Shan-Shan Rao, Feng Chen, Xiang-Ping Yang, Wan-Li Ma, Hong Ye
BACKGROUND: Mucus overproduction is an important feature of asthma. Interleukin (IL)-4 is required for allergen-induced airway inflammation and mucus production. MUC5AC gene expression is regulated by transcript factors NF-κB. The intracellular Ca(2+) ([Ca(2+)]i) signal is required for activation of NF-κB. The transient receptor potential canonical 1 (TRPC1) channel has been shown to contribute for agonist-stimulated Ca(2+) influx in some types of cells. However, the relationships among IL-4, TRPC1 and mucus overproduction in bronchial epithelial cells (BECs) in asthma are poorly understood...
September 20, 2017: Respiratory Research
https://www.readbyqxmd.com/read/28930982/development-of-a-human-preclinical-model-of-osteoclastogenesis-from-peripheral-blood-monocytes-co-cultured-with-breast-cancer-cell-lines
#14
Laura Mercatali, Chiara Spadazzi, Giacomo Miserocchi, Chiara Liverani, Alessandro De Vita, Alberto Bongiovanni, Federica Recine, Dino Amadori, Toni Ibrahim
The crosstalk between tumor cells and bone cells in the bone microenvironment is crucial to understanding the mechanism of bone metastasis formation. We developed an in vitro fully human preclinical model of a co-culture of breast cancer cells and monocytes undergoing differentiation towards osteoclasts. We optimized a model of osteoclastogenesis starting from a sample of peripheral blood collected from healthy donors. Peripheral blood mononuclear cells (PBMCs) were first separated by density gradient centrifugation, seeded at a high density and induced to differentiate by adding two growth factors (GFs): receptor activator of nuclear factor-κB ligand (RANKL) and macrophage colony-stimulating factor (MCSF)...
September 13, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/28930082/cryptosporidium-spp-and-giardia-sp-in-aquatic-mammals-in-northern-and-northeastern-brazil
#15
João Carlos Borges, Danielle Dos Lima, Edson Moura da Silva, André Lucas de Oliveira Moreira, Miriam Marmontel, Vitor Luz Carvalho, Rodrigo de Amaral, Stella Maris Lazzarini, Leucio Câmara Alves
Cryptosporidium and Giardia are protozoans that can infect humans and wild and domestic animals. Due to the growing importance of diseases caused by protozoan parasites in aquatic species, we aimed to evaluate the frequency of infection by Cryptosporidium spp. and Giardia sp. in aquatic and marine mammals in the northern and northeastern regions of Brazil. We collected 553 fecal samples from 15 species of wild-ranging and captive aquatic mammals in northern and northeastern Brazil. All samples were analyzed by the Kinyoun technique for identification of Cryptosporidium spp...
September 20, 2017: Diseases of Aquatic Organisms
https://www.readbyqxmd.com/read/28929253/positive-rheotaxis-extended-drop-a-one-step-procedure-to-select-and-recover-sperm-with-mature-chromatin-for-intracytoplasmic-sperm-injection
#16
Hamilton De Martin, Marcello S Cocuzza, Bruno C Tiseo, Guilherme J A Wood, Eduardo P Miranda, Pedro A A Monteleone, José Maria Soares, Paulo C Serafini, Miguel Srougi, Edmund C Baracat
PURPOSE: The purpose of this study was to develop a novel one-step ICSI approach to select sperm with better chromatin maturity than the conventional method. METHODS: This was a pilot diagnostic study, which prospectively recruited men during a 6-month period in a University-affiliated infertility centre. Forty consecutive semen samples were provided for analysis. The positive rheotaxis extended drop (PRED) was set up creating a pressure and viscosity gradient. Each semen sample was divided into four aliquots: one aliquot for density gradient centrifugation (DGC), two aliquots for PRED (fresh semen (PRED-FS) and processed semen (PRED-DGC)), and one aliquot as the control (FS)...
September 19, 2017: Journal of Assisted Reproduction and Genetics
https://www.readbyqxmd.com/read/28929229/a-novel-method-for-concentration-of-porcine-reproductive-and-respiratory-syndrome-virus-from-the-environmental-samples-using-self-aggregating-peptide-tagged-cd151-binding-capture
#17
Yang Zong, Xiaoyin Zong, Wenlong Xia, Zhi Wu, Guangya Li, Yangyang Li, Xinyu Zhang, Xiaoli Xia, Huaichang Sun
Environmental surveillance of porcine reproductive and respiratory syndrome virus (PRRSV) represents a key issue in control of the disease. CD151 has recently been recognized as one of several receptors for PRRSV. We describe here a novel method for concentration of PRRSV from the environmental samples by CD151-binding capture. After fusion to self-aggregating peptide ELK16, the large extracellular loop (LEL) of porcine CD151 and its two segments (namely N63 and C63) were expressed in E. coli as protein aggregates...
September 19, 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28928845/exosomes-derived-from-bone-marrow-stromal-cells-decrease-the-sensitivity-of-leukemic-cells-to-etoposide
#18
Jianling Wang, Dong Li, Yong Zhuang, Jinqiu Fu, Xue Li, Qing Shi, Xiuli Ju
The aim of the study was to investigate the effect of exosomes derived from bone marrow stromal cells (BM-SCs) on the chemoresistant characteristics of nalm-6 cells treated with etoposide (VP16). The present study isolated exosomes from BM-SC-conditioned medium by using standard differential centrifugation steps and detected the expression of 70 kilodalton heat shock proteins (HSP70) and lysosomal-associated membrane protein 3 (CD63) in exosomes by western blot analysis. Nalm-6 cells were co-cultured with exosomes in the presence of VP16...
September 2017: Oncology Letters
https://www.readbyqxmd.com/read/28927700/the-art-of-separation-and-adsorption-historical-review-of-apheresis-in-japan
#19
REVIEW
Hidenori Matsuo
Apheresis therapy was first introduced into Japan from the United State as plasmapheresis by a centrifuge method. However, the invention of hollow fiber has subsequently lead to a membrane plasma separation. Selective removal of the plasma or cell component has been improved and matured in clinical application. Therapeutic apheresis has progressed and diversified with the development of technology for membrane separation by hollow fiber and adsorption with a physicochemical adsorbent in Japan.
September 5, 2017: Transfusion and Apheresis Science
https://www.readbyqxmd.com/read/28927487/ultrasonically-dispersed-ionic-liquid-based-microextraction-of-lead-in-biological-samples-of-malnourished-children-prior-to-analysis-by-flame-atomic-absorption-spectrometry
#20
Sehrish Talpur, Tasneem Gul Kazi, Hassan Imran Afridi, Farah Naz Talpur, Sooraj Nizamani, Anjum Lashari, Asma Akhtar, Mustafa Khan
In the present study, a simple ultrasonically dispersed modified liquid-phase microextraction method was developed for the extraction of lead (Pb) from blood and scalp hair samples of malnourished children (MNC). The complexation of Pb was executed by means of the complexing agent, ammonium pyrrolidinedithiocarbamate (APDC), whereas extraction was carried out through the ionic liquid (IL), 1-butyl-3-methylimidazolium hexafluorophosphate. Ultrasound energy was used for the dispersion and extraction of the metal complex into an IL because it enhances the extraction of the metal complex into infinite IL drops at a temperature range of 40–80°C for 1–5 min...
September 19, 2017: Journal of AOAC International
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