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https://www.readbyqxmd.com/read/29197602/continuous-flow-perfused-cadaver-model-for-endovascular-training-research-and-development
#1
Amrita Sarkar, Richa Kalsi, Joseph D Ayers, Charles B Drucker, Vaidehi Kaushal, William Sutton, Robert S Crawford
BACKGROUND: Endovascular simulation employing computer, animal, and static models are common and useful adjuncts for teaching endovascular procedures and developing novel, complex endovascular techniques. Unfortunately, these models lack realistic haptic feedback and thus do not faithfully replicate many of the technical challenges associated with clinical endovascular procedures (e.g., arterial calcification, rigidity, and stenosis). We sought to develop a realistic and reproducible perfused cadaver model for endovascular training, device development and research...
November 29, 2017: Annals of Vascular Surgery
https://www.readbyqxmd.com/read/29196770/effect-of-gravity-acceleration-on-choroidal-and-retinal-nerve-fiber-layer-thickness-a-swept-source-optical-coherence-tomography-study
#2
Dong Yoon Kim, Jiho Song, Jin Young Kim, Kyungpil Choi, Sungmin Hyung, Ju Byung Chae
Purpose: The purpose of this study was to evaluate the effect of gravity acceleration on choroidal and retinal nerve fiber layer (RNFL) thickness using swept-source optical coherence tomography (SS-OCT). Methods: Thirteen healthy volunteers who planned to participate in human centrifuge training as part of the flight surgeon selection process enrolled this study. During centrifuge training, gravity was gradually increased up to six times that of sea level. All subjects underwent complete ophthalmologic examination and three-dimensional wide-scanning SS-OCT imaging (DRI OCT-1 Atlantis; Topcon, Tokyo, Japan)...
December 1, 2017: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/29192644/automated-centrifugal-therapeutic-plasma-exchange-option-for-guillain-barre-syndrome-a-report-from-calabar-nigeria
#3
O E Iheanacho, C Chimeziem, B S Sachais, P A Shi
Therapeutic plasma exchange (TPE) is performed frequently and effectively in developed countries, whereas the reverse is the case in developing countries. Guillain-Barre syndrome (GBS), synonymous with acute inflammatory demyelinating polyneuropathy, is an important indication for TPE, but this is rarely administered in the treatment of such patients in Nigeria due to lack of such automated facility, limited expertise, and high cost. This report therefore presents an uncommon case of GBS in which automated TPE was utilized in the management, with the aims of highlighting the current status and challenges of therapeutic apheresis services in Nigeria...
October 2017: Nigerian Journal of Clinical Practice
https://www.readbyqxmd.com/read/29163938/understanding-biological-mechanisms-underlying-adverse-birth-outcomes-in-developing-countries-protocol-for-a-prospective-cohort-amanhi-bio-banking-study
#4
Abdullah H Baqui, Rasheda Khanam, Mohammad Sayedur Rahman, Aziz Ahmed, Hasna Hena Rahman, Mamun Ibne Moin, Salahuddin Ahmed, Fyezah Jehan, Imran Nisar, Atiya Hussain, Muhammad Ilyas, Aneeta Hotwani, Muhammad Sajid, Shahida Qureshi, Anita Zaidi, Sunil Sazawal, Said M Ali, Saikat Deb, Mohammed Hamad Juma, Usha Dhingra, Arup Dutta, Shaali Makame Ame, Caroline Hayward, Igor Rudan, Mike Zangenberg, Donna Russell, Sachiyo Yoshida, Ozren Polašek, Alexander Manu, Rajiv Bahl
Objectives: The AMANHI study aims to seek for biomarkers as predictors of important pregnancy-related outcomes, and establish a biobank in developing countries for future research as new methods and technologies become available. Methods: AMANHI is using harmonised protocols to enrol 3000 women in early pregnancies (8-19 weeks of gestation) for population-based follow-up in pregnancy up to 42 days postpartum in Bangladesh, Pakistan and Tanzania, with collection taking place between August 2014 and June 2016...
December 2017: Journal of Global Health
https://www.readbyqxmd.com/read/29111686/centrifugation-controlled-thermal-convection-and-its-application-to-rapid-microfluidic-polymerase-chain-reaction-devices
#5
Masato Saito, Kazuya Takahashi, Yuichiro Kiriyama, Wilfred Villariza Espulgar, Hiroshi Aso, Tadanobu Sekiya, Yoshikazu Tanaka, Tsuneo Sawazumi, Satoshi Furui, Eiichi Tamiya
Here, we report the developed cyclo olefin polymer (COP) microfluidic chip on a fabricated rotating heater stage that utilizes centrifugation-assisted thermal cycle in a ring-structured microchannel for polymerase chain reaction (PCR). The PCR solution could be driven by thermal convection and continuously exchanged high/low temperatures in a ring structured microchannel without the use of typical syringe pump. More importantly, the flow rate was controlled by the relative gravitational acceleration only. The platform enables amplification within 10 min at 5G and has a detection limit of 70...
December 5, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/29046176/dysrhythmias-in-laypersons-during-centrifuge-simulated-suborbital-spaceflight
#6
Rahul Suresh, Rebecca S Blue, Charles H Mathers, Tarah L Castleberry, James M Vanderploeg
INTRODUCTION: There are limited data on cardiac dysrhythmias in laypersons during hypergravity exposure. We report layperson electrocardiograph (ECG) findings and tolerance of dysrhythmias during centrifuge-simulated suborbital spaceflight. METHODS: Volunteers participated in varied-length centrifuge training programs of 2-7 centrifuge runs over 0.5-2 d, culminating in two simulated suborbital spaceflights of combined +Gz and +Gx (peak +4.0 Gz, +6.0 Gx, duration 5 s)...
November 1, 2017: Aerospace Medicine and Human Performance
https://www.readbyqxmd.com/read/29040513/targeted-metabolomics-reveals-reduced-levels-of-polyunsaturated-choline-plasmalogens-and-a-smaller-dimethylarginine-arginine-ratio-in-the-follicular-fluid-of-patients-with-a-diminished-ovarian-reserve
#7
J M Chao de la Barca, T Boueilh, G Simard, L Boucret, V Ferré-L'Hotellier, L Tessier, C Gadras, P E Bouet, P Descamps, V Procaccio, P Reynier, P May-Panloup
STUDY QUESTION: Does the metabolomic profile of the follicular fluid (FF) of patients with a diminished ovarian reserve (DOR) differ from that of patients with a normal ovarian reserve (NOR)? SUMMARY ANSWER: The metabolomic signature of the FF reveals a significant decrease in polyunsaturated choline plasmalogens and methyl arginine transferase activity in DOR patients compared to NOR patients. WHAT IS KNOWN ALREADY: The composition of the FF reflects the exchanges between the oocyte and its microenvironment during its acquisition of gametic competence...
November 1, 2017: Human Reproduction
https://www.readbyqxmd.com/read/29019959/recent-advances-in-nanoparticle-concentration-and-their-application-in-viral-detection-using-integrated-sensors
#8
REVIEW
Brian M Dincau, Yongkuk Lee, Jong-Hoon Kim, Woon-Hong Yeo
Early disease diagnostics require rapid, sensitive, and selective detection methods for target analytes. Specifically, early viral detection in a point-of-care setting is critical in preventing epidemics and the spread of disease. However, conventional methods such as enzyme-linked immunosorbent assays or cell cultures are cumbersome and difficult for field use due to the requirements of extensive lab equipment and highly trained personnel, as well as limited sensitivity. Recent advances in nanoparticle concentration have given rise to many novel detection methodologies, which address the shortcomings in modern clinical assays...
October 11, 2017: Sensors
https://www.readbyqxmd.com/read/28947321/causes-consequences-and-management-of-sample-hemolysis-in-the-clinical-laboratory
#9
REVIEW
Laura Heireman, Pieter Van Geel, Lorenz Musger, Evelien Heylen, Wim Uyttenbroeck, Boris Mahieu
Preanalytical hemolysis of blood samples is a common problem in medical practice, especially in emergency departments. Several potential influences on sample hemolysis have been investigated, including sampling techniques, centrifugation and sample transport. In particular, the use of intravenous catheters and the vacuum sampling technique have often been demonstrated to provoke hemolysis. Other factors playing a role include the use of inappropriate puncture sites, complicated blood sampling, prolonged tourniquet application, underfilling of tubes and excessive shaking of specimens...
September 22, 2017: Clinical Biochemistry
https://www.readbyqxmd.com/read/28720183/g-loc-warning-algorithms-based-on-emg-features-of-the-gastrocnemius-muscle
#10
Sungho Kim, Taehwan Cho, Yongkyun Lee, Hyojin Koo, Booyong Choi, Dongsoo Kim
BACKGROUND: G-induced loss of consciousness (G-LOC) is mainly caused by failure to sustain an oxygenated blood supply to the pilot's brain because of the sudden acceleration in the direction of the +Gz axis, and is considered a critical safety issue. The purpose of this study was to develop G-LOC warning algorithms based on monitoring electromyograms (EMG) of the gastrocnemius muscle on the calf. METHODS: EMG data was retrieved from a total of 67 pilots and pilot trainees of the Korean Air Force during high-G training on a human centrifugal simulator...
August 1, 2017: Aerospace Medicine and Human Performance
https://www.readbyqxmd.com/read/28641681/the-effects-of-training-on-anxiety-and-task-performance-in-simulated-suborbital-spaceflight
#11
Rebecca S Blue, Frederick Bonato, Kimberly Seaton, Andrea Bubka, Johnené L Vardiman, Charles Mathers, Tarah L Castleberry, James M Vanderploeg
INTRODUCTION: In commercial spaceflight, anxiety could become mission-impacting, causing negative experiences or endangering the flight itself. We studied layperson response to four varied-length training programs (ranging from 1 h-2 d of preparation) prior to centrifuge simulation of launch and re-entry acceleration profiles expected during suborbital spaceflight. We examined subject task execution, evaluating performance in high-stress conditions. We sought to identify any trends in demographics, hemodynamics, or similar factors in subjects with the highest anxiety or poorest tolerance of the experience...
July 1, 2017: Aerospace Medicine and Human Performance
https://www.readbyqxmd.com/read/28555125/determinants-of-motion-sickness-in-tilting-trains-coriolis-cross-coupling-stimuli-and-tilt-delay
#12
Giovanni Bertolini, Meek Angela Durmaz, Kim Ferrari, Alexander Küffer, Charlotte Lambert, Dominik Straumann
Faster trains require tilting of the cars to counterbalance the centrifugal forces during curves. Motion sensitive passengers, however, complain of discomfort and overt motion sickness. A recent study comparing different control systems in a tilting train, suggested that the delay of car tilts relative to the curve of the track contributes to motion sickness. Other aspects of the motion stimuli, like the lateral accelerations and the car jitters, differed between the tested conditions and prevented a final conclusion on the role of tilt delay...
2017: Frontiers in Neurology
https://www.readbyqxmd.com/read/28539143/incidence-of-g-induced-loss-of-consciousness-and-almost-loss-of-consciousness-in-the-royal-air-force
#13
Ellen Slungaard, Judith McLeod, Nicholas D C Green, Amit Kiran, Di J Newham, Stephen D R Harridge
INTRODUCTION: Exposure to sustained +Gz acceleration with inadequate G protection can result in G-induced loss of consciousness (G-LOC) or almost loss of consciousness (A-LOC). The UK Royal Air Force (RAF) last conducted a survey of G-LOC within their military aircrew in 2005 with interventions subsequently introduced. The aim of this study was to repeat the 2005 survey in order to evaluate the impact of those interventions. METHODS: An anonymous questionnaire requesting details of G-LOC and A-LOC events was mailed to all RAF pilots (N = 1878) and weapons systems operators (WSOs) (N = 473), irrespective of aircraft currently flown...
June 1, 2017: Aerospace Medicine and Human Performance
https://www.readbyqxmd.com/read/28459050/assessing-volumetric-absorptive-microsampling-coupled-with-stable-isotope-dilution-assay-and-liquid-chromatography-tandem-mass-spectrometry-as-potential-diagnostic-tool-for-whole-blood-5-methyltetrahydrofolic-acid
#14
Markus Kopp, Michael Rychlik
Volumetric absorptive microsamplers (VAMS) have been developed recently as a promising tool for clinical blood sampling. Compared to dried blood spot samples analyzed by accurate stable isotope dilution assays (SIDAs), the new technique could provide further substantial miniaturizing of folate assays by eliminating hematocrit effects and uneven analyte distribution within the sample. Herein, we present a miniaturized SIDA coupled with LC-MS/MS measurement of 5-methyltetrahydrofolic acid as main folate vitamer in whole blood (WB) using [(13)C5]-5-methyltetrahydrofolic acid as internal standard...
2017: Frontiers in Nutrition
https://www.readbyqxmd.com/read/28262856/resting-serum-concentration-of-high-sensitivity-c-reactive-protein-hs-crp-in-sportsmen-and-untrained-male-adults
#15
COMPARATIVE STUDY
F A Niyi-Odumosu, O A Bello, S A Biliaminu, B V Owoyele, T O Abu, O L Dominic
There is an inverse relationship between regular physical activity and concentration of serum inflammatory markers, with variations in resting CRP in trained and untrained subjects. The effect of acute and prolonged exercises has been studied on inflammatory markers with dearth of information and controversies on the resting serum values of high sensitivity CRP (hs-CRP). Therefore, this study sought to identify and compare variations that occur in serum levels of high sensitivity CRP in groups of sportsmen (6) and physically active untrained subjects...
March 6, 2017: Nigerian Journal of Physiological Sciences: Official Publication of the Physiological Society of Nigeria
https://www.readbyqxmd.com/read/28182858/effect-of-exercise-induced-dehydration-on-circulatory-markers-of-oxidative-damage-and-antioxidant-capacity
#16
Vincent P Georgescu, Tacito P de Souza Junior, Christian Behrens, Marcelo P Barros, Carlos Alves Bueno, Alan C Utter, Lisa S McAnulty, Steven R McAnulty
Dehydration is a common event associated with exercise. However, few studies have examined the effects of dehydration on plasma redox status in humans. Eighty-two athletes were recruited and baseline anthropometrics and blood samples were obtained. Athletes then engaged in a dehydration protocol, training until 3% of preweight body mass was lost. Athletes returned to the lab and had postdehydration blood collected. Athletes then consumed an isotonic drink until pre-exercise body weight was reestablished. Blood was then recollected (1 h post full rehydration (PFR))...
February 9, 2017: Applied Physiology, Nutrition, and Metabolism, Physiologie Appliquée, Nutrition et Métabolisme
https://www.readbyqxmd.com/read/28107395/prevalence-of-pre-analytical-errors-in-clinical-chemistry-diagnostic-labs-in-sulaimani-city-of-iraqi-kurdistan
#17
Dereen Najat
BACKGROUND: Laboratory testing is roughly divided into three phases: a pre-analytical phase, an analytical phase and a post-analytical phase. Most analytical errors have been attributed to the analytical phase. However, recent studies have shown that up to 70% of analytical errors reflect the pre-analytical phase. The pre-analytical phase comprises all processes from the time a laboratory request is made by a physician until the specimen is analyzed at the lab. Generally, the pre-analytical phase includes patient preparation, specimen transportation, specimen collection and storage...
2017: PloS One
https://www.readbyqxmd.com/read/28095952/neck-and-shoulder-muscle-activation-among-experienced-and-inexperienced-pilots-in-gz-exposure
#18
Tuomas Honkanen, Juha Oksa, Matti J Mäntysaari, Heikki Kyröläinen, Janne Avela
BACKGROUND: The aim of the present study was to compare differences in electromyography (EMG) activation of the neck and shoulder muscles between groups of inexperienced and experienced pilots during controlled +Gz exposure in a centrifuge. METHODS: The subjects were volunteer cadets (inexperienced group) and lieutenants (experienced group) undergoing their first centrifuge training. The first group did not have any high performance aircraft (HPA) experience, while the latter one had a 1-yr experience of intense flying of HPA...
February 1, 2017: Aerospace Medicine and Human Performance
https://www.readbyqxmd.com/read/28064225/influence-of-mechanical-force-on-bone-matrix-proteins-in-ovariectomised-mice-and-osteoblast-like-mc3t3-e1-cells
#19
Meng Zhang, Shintaro Ishikawa, Tomoko Inagawa, Hideshi Ikemoto, Shiyu Guo, Masataka Sunagawa, Tadashi Hisamitsu
AIM: To investigate the effect of mechanical stress on periostin and semaphorin-3A expression in a murine model of postmenopausal osteoporosis and in osteoblast-like MC3T3-E1 cells. MATERIALS AND METHODS: Female mice were divided into three groups and treated with a sham operation, ovariectomy (OVX) or OVX plus treadmill training (OVX+Run). After 10 weeks, tibias were used for histological analysis. MC3T3-E1 cells were burdened by mechanical stress using a centrifuge or were treated with periostin, and the production of biologically-active semaphorin-3A was examined in vitro...
January 2, 2017: In Vivo
https://www.readbyqxmd.com/read/27956775/relationship-between-hepcidin-and-oxidant-antioxidant-status-in-calves-with-suspected-neonatal-septicemia
#20
E E Erkilic, H M Erdogan, M Ogun, A H Kirmizigul, E Gokce, M Kuru, A Kukurt
AIM: This study has been conducted for the purpose of determining serum hepcidin, total antioxidant status (TAS), total oxidant status (TOS), and Fe levels in calves with suspected neonatal septicemia before and after treatment and the clinical significance of hepcidin in calves with suspected neonatal septicemia. MATERIALS AND METHODS: The study material consisted of 15 calves of different ages and sexes brought to the Training, Research and Application Center at the Kafkas University Faculty of Veterinary Medicine with suspected neonatal septicemia...
November 2016: Veterinary World
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