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https://www.readbyqxmd.com/read/29439656/genetic-diversity-and-ex-situ-conservation-of-loropetalum-subcordatum-an-endangered-species-endemic-to-china
#1
Bai-Jun Li, Jie-Yu Wang, Zhong-Jian Liu, Xue-Ying Zhuang, Jiu-Xiang Huang
BACKGROUND: Loropetalum subcordatum is an endangered species endemic to China that is characterized by narrow distribution, small population size, and delayed fertilization. However, the genetic diversity of the entire extant natural and ex situ populations has not been assessed to date. In this study, we evaluated the genetic diversity and structure of six natural populations and a single ex situ population (the only known ex situ population of L. subcordatum) using sequence-related amplified polymorphism data...
February 13, 2018: BMC Genetics
https://www.readbyqxmd.com/read/29408208/effect-of-glucocorticoids-on-mirna-expression-spectrum-of-rat-femoral-head-microcirculation-endothelial-cells
#2
Ju'an Yue, Fuyin Wan, Qidong Zhang, Pengfei Wen, Liming Cheng, Ping Li, Wanshou Guo
The study profiled the differential miRNA expression from femoral head bone microvascular endothelial cells (BMECs) between model group and control group to explore the pathogenesis of steroid-induced osteonecrosis of femoral head (ONFH). Twenty 8-week-old Female Sprague-Dawley (SD) rats were randomly divided into control and model groups. Rats in model group received an intraperitoneal injection of 20-μg/kg lipopolysaccharide (LPS) at an interval of 24 h. Then, 24 h later, rats received three doses of 40-mg/kg methylprednisolone by intramuscular injection at intervals of 24 h...
February 2, 2018: Gene
https://www.readbyqxmd.com/read/29389126/quasi-2d-heisenberg-antiferromagnets-cux-pyz-2-bf4-with-x-cl-and-br
#3
Mariusz Kubus, Arianna Lanza, Rebecca Scatena, Leonardo H R Dos Santos, Björn Wehinger, Nicola Casati, Christoph Fiolka, Lukas Keller, Piero Macchi, Christian Rüegg, Karl W Krämer
Two Cu2+ coordination polymers [CuCl(pyz)2](BF4) 1 and [CuBr(pyz)2](BF4) 2 (pyz = pyrazine) were synthesized in the family of quasi two-dimensional (2D) [Cu(pyz)2]2+ magnetic networks. The layer connectivity by monatomic halide ligands results in significantly shorter interlayer distances. Structures were determined by single-crystal X-ray diffraction. Temperature-dependent X-ray diffraction of 1 revealed rigid [Cu(pyz)2]2+ layers that do not expand between 5 K and room temperature, whereas the expansion along the c-axis amounts to 2%...
February 1, 2018: Inorganic Chemistry
https://www.readbyqxmd.com/read/29374817/differential-effects-of-spla2-gv-and-gx-on-cellular-proliferation-and-lipid-accumulation-in-ht29-colon-cancer-cells
#4
Wei Hsum Yap, Su Wen Phang, Nafees Ahmed, Yang Mooi Lim
Secretory phospholipase A2 (sPLA2) group of enzymes have been shown to hydrolyze phospholipids, among which sPLA2 Group V (GV) and Group X (GX) exhibit high selectivity towards phosphatidylcholine-rich cellular plasma membranes. The enzymes have recently emerged as key regulators in lipid droplets formation and it is hypothesized that sPLA2-GV and GX enhanced cell proliferation and lipid droplet accumulation in colon cancer cells (HT29). In this study, cell viability and lipid droplet accumulation were assessed by Resazurin assay and Oil-Red-O staining...
January 27, 2018: Molecular and Cellular Biochemistry
https://www.readbyqxmd.com/read/29355814/development-of-a-high-efficient-biocatalyst-by-oriented-covalent-immobilization-of-a-novel-recombinant-2-n-deoxyribosyltransferase-from-lactobacillus-animalis
#5
Mariana B Méndez, Cintia W Rivero, Fernando López-Gallego, José M Guisán, Jorge A Trelles
The 2'-N-deoxyribosyltransferases [NDT; EC 2.4.2.6] are a group of enzymes widely used as biocatalysts for nucleoside biosynthesis. In this work, the molecular cloning, expression and purification of a novel NDT from Lactobacillus animalis (LaNDT) have been reported. On the other hand, biocatalyst stability has been significantly enhanced by multipoint covalent immobilization using a hetero-functional support activated with nickel-chelates and glyoxyl groups. The immobilized enzyme could be reused for more than 300 h and stored during almost 3 months without activity loss...
January 16, 2018: Journal of Biotechnology
https://www.readbyqxmd.com/read/29348673/quantification-of-massively-parallel-sequencing-libraries-a-comparative-study-of-eight-methods
#6
Christian Hussing, Marie-Louise Kampmann, Helle Smidt Mogensen, Claus Børsting, Niels Morling
Quantification of massively parallel sequencing libraries is important for acquisition of monoclonal beads or clusters prior to clonal amplification and to avoid large variations in library coverage when multiple samples are included in one sequencing analysis. No gold standard for quantification of libraries exists. We assessed eight methods of quantification of libraries by quantifying 54 amplicon, six capture, and six shotgun fragment libraries. Chemically synthesized double-stranded DNA was also quantified...
January 18, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29311306/mechanism-specific-assay-design-facilitates-the-discovery-of-nav1-7-selective-inhibitors
#7
Tania Chernov-Rogan, Tianbo Li, Gang Lu, Henry Verschoof, Kuldip Khakh, Steven W Jones, Maureen H Beresini, Chang Liu, Daniel F Ortwine, Steven J McKerrall, David H Hackos, Daniel Sutherlin, Charles J Cohen, Jun Chen
Many ion channels, including Nav1.7, Cav1.3, and Kv1.3, are linked to human pathologies and are important therapeutic targets. To develop efficacious and safe drugs, subtype-selective modulation is essential, but has been extremely difficult to achieve. We postulate that this challenge is caused by the poor assay design, and investigate the Nav1.7 membrane potential assay, one of the most extensively employed screening assays in modern drug discovery. The assay uses veratridine to activate channels, and compounds are identified based on the inhibition of veratridine-evoked activities...
January 8, 2018: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/29226074/characterization-of-auxiliary-iron-sulfur-clusters-in-a-radical-s-adenosylmethionine-enzyme-pqqe-from-methylobacterium-extorquens-am1
#8
Natsaran Saichana, Katsuyuki Tanizawa, Hiroshi Ueno, Jiří Pechoušek, Petr Novák, Jitka Frébortová
PqqE is a radical S-adenosyl-l-methionine (SAM) enzyme that catalyzes the initial reaction of pyrroloquinoline quinone (PQQ) biosynthesis. PqqE belongs to the SPASM (subtilosin/PQQ/anaerobic sulfatase/mycofactocin maturating enzymes) subfamily of the radical SAM superfamily and contains multiple Fe-S clusters. To characterize the Fe-S clusters in PqqE from Methylobacterium extorquens AM1, Cys residues conserved in the N-terminal signature motif (CX 3 CX 2C) and the C-terminal seven-cysteine motif (CX 9-15 GX 4 CX n CX 2 CX 5 CX 3 CX n C; n = an unspecified number) were individually or simultaneously mutated into Ser...
December 2017: FEBS Open Bio
https://www.readbyqxmd.com/read/29212734/predictors-of-response-and-survival-in-locally-advanced-adenocarcinoma-of-the-pancreas-following-neoadjuvant-gtx-with-or-without-radiation-therapy
#9
William H Sherman, Elizabeth Hecht, David Leung, Kyung Chu
LESSONS LEARNED: There is no presenting parameter that predicts the success of neoadjuvant therapy for pancreatic cancer.Despite the images on scans following neoadjuvant therapy, all patients should be evaluated, because inflammation following radiation therapy (RT) may overstate the extent of tumor and vascular involvement. BACKGROUND: In patients presenting with locally advanced pancreatic adenocarcinoma deemed unresectable by two pancreatic cancer surgeons, we analyzed presenting tumor size, extent of vascular involvement, tumor markers, response to neoadjuvant gemcitabine (G), docetaxel (T), and capecitabine (X) with or without additional chemoradiotherapy with GX on R0 resection rates (≥2 mm margins), and survival...
December 6, 2017: Oncologist
https://www.readbyqxmd.com/read/29172486/a-stable-isotope-dilution-nanoflow-liquid-chromatography-tandem-mass-spectrometry-assay-for-the-simultaneous-detection-and-quantification-of-glyoxal-induced-dna-cross-linked-adducts-in-leukocytes-from-diabetic-patients
#10
Hauh-Jyun Candy Chen, Ya-Lang Chang, Yi-Chun Teng, Chiung-Fong Hsiao, Tsai-Shiuan Lin
Glyoxal is a bifunctional electrophile capable of cross-linking DNA. Although it is present in foods and from the environment, endogenous formation of glyoxal occurs through metabolism of carbohydratesand oxidation of lipids and nucleic acids. Plasma concentrations of glyoxal are elevatedin in diabetes mellitus patients compared to non-diabetics. The most abundant 2'-deoxyribonucleoside adducts cross-linked by glyoxal are dG-gx-dC, dG-gx-dA, and dG-gx-dG. These DNA cross-links can be mutagenic by damaging the integrity of the DNA structure...
November 25, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/29168858/ferromagnetic-coupling-in-copper-benzimidazole-chloride-structural-mass-spectrometry-magnetism-and-dft-studies
#11
Xing-Xing Shi, Yuexing Zhang, Qiu-Jie Chen, Zheng Yin, Xue-Li Chen, Zhenxing Wang, Zhong-Wen Ouyang, Mohamedally Kurmoo, Ming-Hua Zeng
Herein, quasi-square planar Cu(II)(Hmbm)Cl2 (CBC, Hmbm = (1-methyl-1H-benzo[d]imidazol-2-yl)methanol) was arranged in a pseudo orthogonal way to form Cl-bridged chains, and further ππ interactions resulted in distorted hexagonal layers. DFT calculations reveal a bond strength order of Cu-Cl > Cu-O/N ≫ CuCl. ESI-MS data reveal several small fragments from CBC, but oligomeric [Cu], [Cu], and [Cu] for non-zero in-source energies; MS data indicates the occurrence of several chemical processes, viz. splitting of the ligand, oligomerization, and redox reaction of alcohol to aldehyde and Cu(II) to Cu(I)...
November 23, 2017: Dalton Transactions: An International Journal of Inorganic Chemistry
https://www.readbyqxmd.com/read/29127731/endoscopic-submucosal-dissection-of-early-gastric-neoplasms-in-the-fornix-using-the-newly-developed-scissors-type-sb-knife-gx
#12
Yuki Sumida, Toshio Kuwai, Sauid Ishaq
Endoscopic submucosal dissection (ESD) using a scissors-type knife, the Stag-Beetle knife (SB knife, Sumitomo Bakelite, Tokyo, Japan), is considered technically easy and safe due to its ability to grasp the target tissue to allow controlled resection without the complex endoscopic tip control required with conventional ESD devices. There are two types of SB knife (short-type and Jr-type), used mainly for esophageal and colorectal neoplasms (1-3). Recently, a new type, SB knife GX, has been developed for gastric ESD...
November 11, 2017: Digestive Endoscopy: Official Journal of the Japan Gastroenterological Endoscopy Society
https://www.readbyqxmd.com/read/29119984/catalytic-fixation-of-atmospheric-carbon-dioxide-by-copper-ii-complexes-of-bidentate-ligands
#13
Sethuraman Muthuramalingam, Themmila Khamrang, Marappan Velusamy, Ramasamy Mayilmurugan
New copper(ii) complexes, [Cu(L1)2(H2O)](ClO4)2, 1 [L1 = 2-pyridin-2-yl-quinoline], [Cu(L2)2(H2O)](ClO4)2, 2 [L2 = 2-pyridin-2-yl-quinoxaline], [Cu(L3)2(H2O)](ClO4)2, 3 [L3 = 6,7-dimethyl-2-pyridin-2-yl-quinoxaline], [Cu(L4)2(H2O)](ClO4)2, 4 [L4 = 4-phenyl-2-pyridin-2-yl-quinoline] and [Cu(L5)2(H2O)](ClO4)2, 5 [L5 = 4-phenyl-2-pyridin-2-yl-quinazoline], were synthesized and characterized as catalysts for selective fixation of atmospheric CO2. The molecular structure of 2 was determined by single-crystal X-ray studies and shown to have an unusual trigonal bipyramid geometry (τ, 0...
November 9, 2017: Dalton Transactions: An International Journal of Inorganic Chemistry
https://www.readbyqxmd.com/read/29107627/optimal-13-c-labeling-of-glycerol-carbon-source-for-precise-flux-estimation-in-escherichia-coli
#14
Yoshihiro Toya, Shugo Ohashi, Hiroshi Shimizu
Glycerol is a promising carbon source for bio-production and is particularly attractive because it is produced in excess as a biodiesel byproduct. Elucidating the flux distribution of glycerol catabolism would greatly aid metabolic engineering, but (13)C-labeling of glycerol has not yet been optimized for precise flux estimations. In this study, an Escherichia coli wild type strain was aerobically cultured using glycerol as the sole carbon source. [1,3-(13)C], [2-(13)C], and [U-(13)C] glycerols were independently mixed with an equal amount of naturally labeled glycerol; these mixtures were used as (13)C-labeled substrates, and flux distributions during exponential growth were estimated based on (13)C-enrichment of proteinogenic amino acids...
October 26, 2017: Journal of Bioscience and Bioengineering
https://www.readbyqxmd.com/read/29094805/synthesis-of-naphthyridine-dimers-with-conformational-restriction-and-binding-to-dna-and-rna
#15
Kazuhiko Nakatani, Nozomi Natsuhara, Yuki Mori, Sanjukta Mukherjee, Bimolendu Das, Asako Murata
One of the important determinants in the efficiency of a molecular interaction is the necessity for conformational changes in host and/or guest molecules upon binding. In small-molecule interactions with nucleic acids, conformational changes on both molecules are often involved, especially in intercalating binding. Mismatch binding ligands (MBLs) we described here consist of two heterocycles that predominantly exist in one conformation, so it is of interest to determine if such molecules can bind to any DNA and RNA structures...
November 2, 2017: Chemistry, An Asian Journal
https://www.readbyqxmd.com/read/29046176/dysrhythmias-in-laypersons-during-centrifuge-simulated-suborbital-spaceflight
#16
Rahul Suresh, Rebecca S Blue, Charles H Mathers, Tarah L Castleberry, James M Vanderploeg
INTRODUCTION: There are limited data on cardiac dysrhythmias in laypersons during hypergravity exposure. We report layperson electrocardiograph (ECG) findings and tolerance of dysrhythmias during centrifuge-simulated suborbital spaceflight. METHODS: Volunteers participated in varied-length centrifuge training programs of 2-7 centrifuge runs over 0.5-2 d, culminating in two simulated suborbital spaceflights of combined +Gz and +Gx (peak +4.0 Gz, +6.0 Gx, duration 5 s)...
November 1, 2017: Aerospace Medicine and Human Performance
https://www.readbyqxmd.com/read/29023423/new-strategy-for-the-immobilization-of-lipases-on-glyoxyl-agarose-supports-production-of-robust-biocatalysts-for-natural-oil-transformation
#17
César A Godoy
Immobilization on Glyoxyl-agarose support (Gx) is one of the best strategies to stabilize enzymes. However, the strategy is difficult to apply at neutral pH when most enzymes are stable and, even when possible, produces labile derivatives. This work contributes to overcoming this hurdle through a strategy that combines solid-phase amination, presence of key additives, and derivative basification. To this end, aminated industrial lipases from Candida artarctica (CAL), Thermomyces lunuginosus (TLL), and the recombinant Geobacillus thermocatenulatus (BTL2) were immobilized on Gx for the first time at neutral pH using anthranilic acid (AA) or DTT as additives (immobilization yields >70%; recovered activities 37...
October 12, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28969070/immunohistochemical-assays-incorporating-sp142-and-22c3-monoclonal-antibodies-for-detection-of-pd-l1-expression-in-nsclc-patients-with-known-status-of-egfr-and-alk-genes
#18
Paweł Krawczyk, Bożena Jarosz, Tomasz Kucharczyk, Anna Grenda, Katarzyna Reszka, Juliusz Pankowski, Kamila Wojas-Krawczyk, Marcin Nicoś, Justyna Szumiło, Tomasz Trojanowski, Janusz Milanowski
Different immunohistochemical (IHC) assays were approved for PD-L1 expression examination on tumor cells in qualification to immune-checkpoint inhibitors therapy in NSCLC patients. These assays have some similarities, but also very serious differences. We assessed 2 IHC tests for PD-L1 expression evaluation in NSCLC tumors with different pathological diagnoses and genetic abnormalities. We enrolled 48 NSCLC patients (median age: 65 years) with known status of EGFR and ALK genes. We compared the effectiveness of PD-L1 expression examination of two IHC assays with 22C3 (Dako) and SP142 antibodies (Ventana)...
September 8, 2017: Oncotarget
https://www.readbyqxmd.com/read/28938202/effect-of-different-diode-laser-wavelengths-on-root-dentin-decontamination-infected-with-enterococcus-faecalis
#19
Caroline Cristina Borges, Carlos Estrela, Fabiane Carneiro Lopes, Regina Guenka Palma-Dibb, Jesus Djalma Pecora, Cyntia Rodrigues De Araújo Estrela, Manoel Damião de Sousa-Neto
The objective of this study was to evaluate the antibacterial effect and the ultrastructural alterations of diode laser with different wavelengths (808nm and 970nm) and its association with irrigating solutions (2.5% sodium hypochlorite and 2% chlorhexidine) in root dentin contaminated by a five days biofilm. Thirteen uniradicular teeth were sectioned into 100 dentin intraradicular blocks. Initially, the blocks were immersed for 5min in 17% EDTA and washed with distilled water for 5min, then samples were sterilized for 30min at 120°C...
November 2017: Journal of Photochemistry and Photobiology. B, Biology
https://www.readbyqxmd.com/read/28923281/genetic-diversity-and-intergenogroup-recombination-events-of-sapoviruses-detected-from-feces-of-pigs-in-japan
#20
Moegi Kuroda, Tsuneyuki Masuda, Mika Ito, Yuki Naoi, Yen Hai Doan, Kei Haga, Shinobu Tsuchiaka, Mai Kishimoto, Kaori Sano, Tsutomu Omatsu, Yukie Katayama, Mami Oba, Hiroshi Aoki, Toru Ichimaru, Fujiko Sunaga, Itsuro Mukono, Hiroshi Yamasato, Junsuke Shirai, Kazuhiko Katayama, Tetsuya Mizutani, Tomoichiro Oka, Makoto Nagai
Sapoviruses (SaV) are enteric viruses infecting humans and animals. SaVs are highly diverse and are divided into multiple genogroups based on structural protein (VP1) sequences. SaVs detected from pigs belong to eight genogroups (GIII, GV, GVI, GVII, GVIII, GIX, GX, and GXI), but little is known about the SaV genogroup distribution in the Japanese pig population. In the present study, 26 nearly complete genome (>6000 nucleotide: nt) and three partial sequences (2429nt, 4364nt, and 4419nt in length, including the entire VP1 coding region) of SaV were obtained from one diarrheic and 15 non-diarrheic porcine feces in Japan via a metagenomics approach...
September 18, 2017: Infection, Genetics and Evolution
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