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https://www.readbyqxmd.com/read/28641681/the-effects-of-training-on-anxiety-and-task-performance-in-simulated-suborbital-spaceflight
#1
Rebecca S Blue, Frederick Bonato, Kimberly Seaton, Andrea Bubka, Johnené L Vardiman, Charles Mathers, Tarah L Castleberry, James M Vanderploeg
INTRODUCTION: In commercial spaceflight, anxiety could become mission-impacting, causing negative experiences or endangering the flight itself. We studied layperson response to four varied-length training programs (ranging from 1 h-2 d of preparation) prior to centrifuge simulation of launch and re-entry acceleration profiles expected during suborbital spaceflight. We examined subject task execution, evaluating performance in high-stress conditions. We sought to identify any trends in demographics, hemodynamics, or similar factors in subjects with the highest anxiety or poorest tolerance of the experience...
July 1, 2017: Aerospace Medicine and Human Performance
https://www.readbyqxmd.com/read/28640584/spectroscopic-evidence-for-a-h-bond-network-at-y356-located-at-the-subunit-interface-of-active-e-coli-ribonucleotide-reductase
#2
Thomas U Nick, Kanchana R Ravichandran, JoAnne Stubbe, Müge Kasanmascheff, Marina Bennati
The reaction catalyzed by E. coli ribonucleotide reductase (RNR) composed of α and β subunits that form an active α2β2 complex is a paradigm for proton-coupled electron transfer (PCET) processes in biological transformations. β2 contains the diferric tyrosyl radical (Y122•) cofactor that initiates a radical transfer (RT) over 35 Å via a specific pathway of amino acids (Y122• ↔ [W48] ↔ Y356 in β2 to Y731 ↔Y730↔ C439 in α2). Experimental evidence exists for co-linear and orthogonal PCET in α2 and β2 respectively...
June 22, 2017: Biochemistry
https://www.readbyqxmd.com/read/28622705/parvus-affects-aluminum-sensitivity-by-modulating-the-structure-of-glucuronoxylan-in-arabidopsis-thaliana
#3
Xiao Fang Zhu, Jiang Xue Wan, Qi Wu, Xu Sheng Zhao, Shao Jian Zheng, Ren Fang Shen
Glucuronoxylan (GX), an important component of hemicellulose in the cell wall, appears to affect aluminum (Al) sensitivity in plants. To investigate the role of GX in cell-wall-localized xylan, we examined the Arabidopsis thaliana parvus mutant in detail. This mutant lacks α-D-glucuronic acid (GlcA) side chains in GX and has greater resistance to Al stress than wild-type (WT) plants. The parvus mutant accumulated lower levels of Al in its roots and cell walls than WT despite having cell wall pectin content and pectin methylesterase (PME) activity similar to those of WT...
June 16, 2017: Plant, Cell & Environment
https://www.readbyqxmd.com/read/28619134/genetic-antigenic-and-pathogenic-characteristics-of-avian-infectious-bronchitis-viruses-genotypically-related-to-793-b-in-china
#4
Zongxi Han, Wenjun Zhao, Yuqiu Chen, Qianqian Xu, Junfeng Sun, Tingting Zhang, Yan Zhao, Shuling Liang, Mengying Gao, Qiuling Wang, Xiangang Kong, Shengwang Liu
In this study, 20 infectious bronchitis virus (IBV) strains, which were genotypically related to 793/B, as assessed by an S1 gene comparison and a complete genomic sequence analysis, were isolated and identified from 2009 to 2014 in China. Phylogenetic analysis, network tree, similarity plot analysis, Recombination Detection Program 4(RDP4) and sequence comparison revealed that 12 of the 20 isolates were likely the reisolated vaccine virus. One isolate, ck/CH/LSD/110857, was shown to have originated from recombination events between H120- and 4/91-like vaccine strains that did not result in changes of antigenicity and pathogenicity...
May 2017: Veterinary Microbiology
https://www.readbyqxmd.com/read/28581288/microporous-lanthanide-metal-organic-framework-constructed-from-lanthanide-metalloligand-for-selective-separation-of-c2h2-co2-and-c2h2-ch4-at-room-temperature
#5
Jing-Xin Ma, Jiarui Guo, Hailong Wang, Bin Li, Tianlin Yang, Banglin Chen
A novel pillar-layer porous lanthanide metal-organic framework [Tb3(ODA)3(BPDC)3Na2]n·Gx (UTSA-222, G = guest molecules) was constructed from an organic ligand [1,1'-biphenyl]-4,4'-dicarboxylate (BPDC(2-)) and a lanthanide metalloligand [Tb(ODA)](+) (H2ODA = oxydiacetic acid). The UTSA-222 contains two-dimensional intersecting channels with the Brunauer-Emmett-Teller surface area and pore volume of 703 m(2) g(-1) and 0.344 cm(3) g(-1), respectively, for the activated sample. It shows moderately high adsorption selectivity for C2H2/CO2 and C2H2/CH4 separations at 1 atm and room temperature...
June 5, 2017: Inorganic Chemistry
https://www.readbyqxmd.com/read/28543855/theoretical-studies-of-the-copper-gyromagnetic-factors-for-three-novel-cu-2-coordination-polymers-with-bi-triazole-ligand
#6
Li-Na Wu, Shao-Yi Wu, Li-Juan Zhang, Xu-Sheng Liu, Li Peng
The copper electron paramagnetic resonance (EPR) gyromagnetic factors are theoretically studied for three novel Cu(2+) coordination polymers [Cu(XL)(NO3 )2 ]n (1), {[Cu(XL)(4,4'-bpy)(NO3 )2 ]•CH3 CN}n (1a) and {[Cu(XL)3 ](NO3 )2 •3.5H2 O}n (2) with bi-triazole ligand (XL)=N,N'-bicyclo[2.2.2]oct-7-ene-2,3,5,6-tetracarboxdiimide bi(1,2,4-triazole) from the high order perturbation calculations of the g factors for a rhombically elongated octahedral 3d(9) group. The order (1 ≤ 1a < 2) of gz can be illustrated by the dominant second-order perturbation term roughly proportional to the square of the covalency factor N...
May 25, 2017: Magnetic Resonance in Chemistry: MRC
https://www.readbyqxmd.com/read/28537544/high-prevalence-of-four-novel-astrovirus-genotype-species-identified-from-rodents-in-china
#7
Kelvin K W To, Wan-Mui Chan, Kenneth S M Li, Carol S F Lam, Zhiwei Chen, Herman Tse, Susanna K P Lau, Patrick C Y Woo, Kwok-Yung Yuen
Astroviruses cause gastrointestinal and neurological infections in humans and animals. Since astrovirus is genetically diverse and different astrovirus genotypes can be found in the same animal species, astrovirus is a potential zoonotic threat to humans. In this study, we screened for astroviruses in rodents from Hong Kong, Hunan and Guangxi. Astrovirus was detected in 11.9 % (67/562) of rectal swab specimens. Phylogenetic analysis of the ORF1b region, which encodes the RdRp, showed that there were four distinct clusters (clusters A, B, C and D)...
May 25, 2017: Journal of General Virology
https://www.readbyqxmd.com/read/28534584/composition-dependent-electronic-energy-relaxation-dynamics-of-metal-domains-as-revealed-by-bimetallic-au144-xagx-sc8h9-60-monolayer-protected-clusters
#8
Hongjun Zheng, Marcus A Tofanelli, Christopher J Ackerson, Kenneth L Knappenberger
We examined the electronic relaxation dynamics for mono and bimetallic Au144-xAgx(SC8H9)60 monolayer-protected clusters (MPCs) using femtosecond time-resolved transient extinction spectroscopy. MPCs provide compositionally well-defined model systems for structure-specific determination of nanoscale electronic properties. Based on pulse-energy-dependent transient extinction data, we quantified electron-phonon coupling constants for three distinct Au144-xAgx(SC8H9)60 MPC samples, where x = 0, 0 < x < 30, and x ∼ 30, as Gx=0 = (1...
June 7, 2017: Physical Chemistry Chemical Physics: PCCP
https://www.readbyqxmd.com/read/28521490/transcript-levels-of-ten-eleven-translocation-type-1-3-in-cervical-cancer-and-non-cancerous-cervical-tissues
#9
Dorota Ewa Bronowicka-Kłys, Andrzej Roszak, Piotr Pawlik, Stefan Sajdak, Anna Sowińska, Paweł Piotr Jagodziński
Decreased expression of ten-eleven translocation (TET1, TET2 and TET3) proteins has been reported in various types of cancer. However, the expression levels of TET proteins in cervical cancer (CC) remain to be elucidated. The present study determined the levels of TET1, TET2 and TET3 transcripts in cancerous (n=80) and non-cancerous cervical tissues (n=41). The results revealed a significant reduction in TET1 transcripts (P=0.0000001) in cervical tissue samples from patients with primary CC compared with samples from control patients...
May 2017: Oncology Letters
https://www.readbyqxmd.com/read/28508481/a-comparison-of-identity-by-descent-and-identity-by-state-matrices-that-are-used-for-genetic-evaluation-and-estimation-of-variance-components
#10
R L Fernando, H Cheng, X Sun, D J Garrick
The genetic covariance matrix conditional on pedigree is proportional to the pedigree-based additive relationship matrix (PARM), which is twice the matrix of identity-by-descent (IBD) probabilities. In genomic prediction, IBD probabilities in the PARM, which are expected genetic similarities between relatives that are derived from the pedigree, are substituted by realized similarities that are derived from genotypes to obtain a genomic additive relationship matrix (GARM). Different definitions of similarity lead to different GARMs, and two commonly used GARMS are the matrix G, which is based on an allele substitution effect model, and the matrix T, which is based on an allele effect model...
June 2017: Journal of Animal Breeding and Genetics, Zeitschrift Für Tierzüchtung und Züchtungsbiologie
https://www.readbyqxmd.com/read/28459004/immobilization-of-calb-on-activated-chitosan-application-to-enzymatic-synthesis-in-supercritical-and-near-critical-carbon-dioxide
#11
José C S Dos Santos, Horacio L Bonazza, Leonardo J B L de Matos, Elizabete A Carneiro, Oveimar Barbosa, Roberto Fernandez-Lafuente, Luciana R B Gonçalves, Hosiberto B de Sant' Ana, Rílvia S Santiago-Aguiar
The objective of this new paper was to evaluate the enzymatic esterification reaction conducted in supercritical or near-critical CO2, catalyzed by immobilized lipase B from Candida antarctica (CALB). The biocatalyst was prepared through the immobilization of CALB by covalent attachment using chitosan sequentially activated with Glycidol, ethylenediamine (EDA) and glutaraldehyde as support. In order to determine the best operational conditions of the esterification reaction (1: 1 (alcohol-acid); biocatalyst content, 10% (by substrate mass); 45 °C), an experimental design (2(3)) was conducted to evaluate the effects of the following parameters: alcohol to oil molar ratios, reaction time and temperature...
March 2017: Biotechnology Reports
https://www.readbyqxmd.com/read/28452738/development-of-lc-ms-ms-method-for-determination-of-the-olanzapine-in-human-plasma
#12
K Sivsivadze, M Jokhadze, P Tushurashvili, T Murtazashvili, N Imnadze
The study aim was to develop a validated selective, reproducible and sensitive Liquid Chromatography - Tandem Mass Spectrometry (LC-MS/MS) method for qualitative and quantitative determination of olanzapin (OLZ ) in human plasma. For isolation was used solid phase extraction with the Gilson GX-271 ASPEC Solid phase extraction system, cartridge Supelclean LC-18 SPE 500mg. Elution solvent contained 40mL of isopropanol, 4mL of concentrated ammonium hydroxide and 156mL of freshly prepared methylene chloride. Samples containing OLZ and IS were chromatographed on AGILENT TECHNOLOGIES 1290 Infinity AGILENT TECHNOLOGIES 6460 Triple quad LC-MS/MS system...
February 2017: Georgian Medical News
https://www.readbyqxmd.com/read/28335440/profile-of-polyphenol-compounds-of-five-muscadine-grapes-cultivated-in-the-united-states-and-in-newly-adapted-locations-in-china
#13
Zheng Wei, Jianming Luo, Yu Huang, Wenfeng Guo, Yali Zhang, Huan Guan, Changmou Xu, Jiang Lu
Polyphenol compositions and concentrations in skins and seeds of five muscadine grapes (cv. "Noble", "Alachua", "Carlos", "Fry", and "Granny Val") cultivated in the United States (Tallahassee-Florida, TA-FL) and South China (Nanning-Guangxi, NN-GX and Pu'er-Yunnan, PE-YN) were investigated, using ultra performance liquid chromatography tandem triple quadrupole time-of-flight mass spectrometry (UPLC Triple TOF MS/MS). Fourteen ellagitannins were newly identified in these muscadine grapes. The grapes grown in NN-GX accumulated higher levels of ellagic acid, methyl brevifolin carboxylate, and ellagic acid glucoside in skins, and penta-O-galloyl-glucose in seeds...
March 14, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28300877/mast-cell-concentration-and-skin-wound-contraction-in-rats-treated-with-ximenia-americana-l
#14
José de Castro Souza Junior Neto, Lígia Reis de Moura Estevão, Liriane Baratella-Evêncio, Marcela Gabriela Feitosa Vieira, Ricardo Santos Simões, Rinaldo Florencio-Silva, Luís Evêncio-Luz, Joaquim Evêncio-Neto
PURPOSE: To evaluate wound contraction and the concentration of mast cells in skin wounds treated with wild plum (Ximenia americana) essential oil-based ointment in rats. METHODS: Sixty rats were submitted to two cutaneous wounds in the thoracic region, on the right and left antimeres. Thereon, they were divided into three groups: GX (wounds treated once a day with hydro alcoholic branch extract of Ximenia americana), GP (wounds that received vehicle), and GC (wounds without product application)...
February 2017: Acta Cirúrgica Brasileira
https://www.readbyqxmd.com/read/28273352/multifactorial-study-and-kinetics-of-signal-development-in-ferrous-methylthymol-blue-gelatin-gel-dosimeters
#15
Kalin I Penev, Kibret Mequanint
PURPOSE: To develop and characterize a ferrous-methylthymol blue-gelatin gel dosimeter with low optical background and appropriate additives for reduced rate of auto-oxidation and diffusion. METHODS: A mixed-level multifactorial design of experiments was used to test the effects of the concentrations of sulfuric acid, 5-nitro-1,10-phenanthroline (Nn), and glyoxal (Gx) on the background absorbance, dose sensitivity, and auto-oxidation of the tested gel dosimeter...
March 8, 2017: Medical Physics
https://www.readbyqxmd.com/read/28260382/fast-separation-and-sensitive-quantitation-of-polymethoxylated-flavonoids-in-the-peels-of-citrus-using-uplc-q-tof-ms
#16
Tian Tian Xing, Xi Juan Zhao, Yi Dan Zhang, Yuan Fang Li
A rapid, sensitive, and efficient ultraperformance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MS) method has been developed to analyze polymethoxylated flavonoids (PMFs) in 14 Citrus peels, including 7 Citrus reticulata (C. reticulata) and 7 Citrus sinensis (C. sinensis). In this study, fast separation can be achieved within 12 min and 42 PMFs have been identified including 33 flavones and 9 flavanones. Most C. reticulata were shown to contain more than 20 PMFs, except Guangxihongpisuanju (GX) containing only 12 PMFs, while most C...
March 15, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28252967/hyperfine-sublevel-correlation-spectroscopy-studies-of-iron-sulfur-cluster-in-rieske-protein-from-green-sulfur-bacterium-chlorobaculum-tepidum
#17
Hiroki Nagashima, Hiraku Kishimoto, Risa Mutoh, Naotaka Terashima, Hirozo Oh-Oka, Genji Kurisu, Hiroyuki Mino
The magnetic properties of the Rieske protein purified from Chlorobaculum tepidum were investigated using electron paramagnetic resonance and hyperfine sublevel correlation spectroscopy (HYSCORE). The g-values of the Fe2S2 center were gx = 1.81, gy = 1.90, and gz = 2.03. Four classes of nitrogen signals were obtained by HYSCORE. Nitrogens 1 and 2 had relatively strong magnetic hyperfine couplings and were assigned as the nitrogen directly ligated to Fe. Nitrogens 3 and 4 had relatively weak magnetic hyperfine couplings and were assigned as the other nitrogen of the His ligands and peptide nitrogen connected to the sulfur atom via hydrogen bonding, respectively...
March 17, 2017: Journal of Physical Chemistry. B
https://www.readbyqxmd.com/read/28093423/medical-and-psychosocial-factors-associated-with-antibiotic-prescribing-in-primary-care-survey-questionnaire-and-factor-analysis
#18
Tau-Hong Lee, Joshua Gx Wong, David Cb Lye, Mark Ic Chen, Victor Wk Loh, Yee-Sin Leo, Linda K Lee, Angela Lp Chow
BACKGROUND: Acute upper respiratory infections (AURI) are the leading causes of antibiotic prescribing in primary care although antibiotics are often not indicated. AIM: To gain an understanding of the knowledge, attitudes, and practices (KAP) of GPs in Singapore and the associated latent factors to guide the implementation of an effective programme to reduce antibiotic use in primary care. DESIGN AND SETTING: An anonymous survey on the KAP of antibiotic use in AURI of GPs in Singapore...
March 2017: British Journal of General Practice: the Journal of the Royal College of General Practitioners
https://www.readbyqxmd.com/read/28081476/electron-spin-relaxation-of-a-boron-containing-heterocyclic-radical
#19
Sandra S Eaton, Kirby Huber, Hanan Elajaili, Joseph McPeak, Gareth R Eaton, Lauren E Longobardi, Douglas W Stephan
Preparation of the stable boron-containing heterocyclic phenanthrenedione radical, (C6F5)2B(O2C14H8), by frustrated Lewis pair chemistry has been reported recently. Electron paramagnetic resonance measurements of this radical were made at X-band in toluene:dichloromethane (9:1) from 10 to 293K, in toluene from 180 to 293K and at Q-band at 80K. In well-deoxygenated 0.1mM toluene solution at room temperature hyperfine splittings from (11)B, four pairs of (1)H, and 5 pairs of (19)F contribute to an EPR spectrum with many resolved lines...
December 30, 2016: Journal of Magnetic Resonance
https://www.readbyqxmd.com/read/28028556/effect-of-intravenous-gadoxetate-disodium-and-flip-angle-on-hepatic-proton-density-fat-fraction-estimation-with-six-echo-gradient-recalled-echo-magnitude-based-mr-imaging-at-3t
#20
Charlie C Park, Gavin Hamilton, Ajinkya Desai, Kevin A Zand, Tanya Wolfson, Jonathan C Hooker, Eduardo Costa, Elhamy Heba, Lisa Clark, Anthony Gamst, Rohit Loomba, Michael S Middleton, Claude B Sirlin
PURPOSE: The aim of the study was to determine in patients undergoing gadoxetate disodium (Gx)-enhanced MR exams whether proton density fat fraction (PDFF) estimation accuracy of magnitude-based multi-gradient-echo MRI (MRI-M) could be improved by using high flip angle (FA) on post-contrast images. MATERIALS AND METHODS: Thirty-one adults with known or suspected hepatic steatosis undergoing 3T clinical Gx-enhanced liver MRI were enrolled prospectively. MR spectroscopy (MRS), the reference standard, was performed before Gx to measure MRS-PDFF...
April 2017: Abdominal Radiology
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