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César A Godoy
Immobilization on Glyoxyl-agarose support (Gx) is one of the best strategies to stabilize enzymes. However, the strategy is difficult to apply at neutral pH when most enzymes are stable and, even when possible, produces labile derivatives. This work contributes to overcoming this hurdle through a strategy that combines solid-phase amination, presence of key additives, and derivative basification. To this end, aminated industrial lipases from Candida artarctica (CAL), Thermomyces lunuginosus (TLL), and the recombinant Geobacillus thermocatenulatus (BTL2) were immobilized on Gx for the first time at neutral pH using anthranilic acid (AA) or DTT as additives (immobilization yields >70%; recovered activities 37...
October 12, 2017: International Journal of Molecular Sciences
Paweł Krawczyk, Bożena Jarosz, Tomasz Kucharczyk, Anna Grenda, Katarzyna Reszka, Juliusz Pankowski, Kamila Wojas-Krawczyk, Marcin Nicoś, Justyna Szumiło, Tomasz Trojanowski, Janusz Milanowski
Different immunohistochemical (IHC) assays were approved for PD-L1 expression examination on tumor cells in qualification to immune-checkpoint inhibitors therapy in NSCLC patients. These assays have some similarities, but also very serious differences. We assessed 2 IHC tests for PD-L1 expression evaluation in NSCLC tumors with different pathological diagnoses and genetic abnormalities. We enrolled 48 NSCLC patients (median age: 65 years) with known status of EGFR and ALK genes. We compared the effectiveness of PD-L1 expression examination of two IHC assays with 22C3 (Dako) and SP142 antibodies (Ventana)...
September 8, 2017: Oncotarget
Caroline Cristina Borges, Carlos Estrela, Fabiane Carneiro Lopes, Regina Guenka Palma-Dibb, Jesus Djalma Pecora, Cyntia Rodrigues De Araújo Estrela, Manoel Damião de Sousa-Neto
The objective of this study was to evaluate the antibacterial effect and the ultrastructural alterations of diode laser with different wavelengths (808nm and 970nm) and its association with irrigating solutions (2.5% sodium hypochlorite and 2% chlorhexidine) in root dentin contaminated by a five days biofilm. Thirteen uniradicular teeth were sectioned into 100 dentin intraradicular blocks. Initially, the blocks were immersed for 5min in 17% EDTA and washed with distilled water for 5min, then samples were sterilized for 30min at 120°C...
September 13, 2017: Journal of Photochemistry and Photobiology. B, Biology
Moegi Kuroda, Tsuneyuki Masuda, Mika Ito, Yuki Naoi, Yen Hai Doan, Kei Haga, Shinobu Tsuchiaka, Mai Kishimoto, Kaori Sano, Tsutomu Omatsu, Yukie Katayama, Mami Oba, Hiroshi Aoki, Toru Ichimaru, Fujiko Sunaga, Itsuro Mukono, Hiroshi Yamasato, Junsuke Shirai, Kazuhiko Katayama, Tetsuya Mizutani, Tomoichiro Oka, Makoto Nagai
Sapoviruses (SaV) are enteric viruses infecting humans and animals. SaVs are highly diverse and are divided into multiple genogroups based on structural protein (VP1) sequences. SaVs detected from pigs belong to eight genogroups (GIII, GV, GVI, GVII, GVIII, GIX, GX, and GXI), but little is known about the SaV genogroup distribution in the Japanese pig population. In the present study, 26 nearly complete genome (>6000 nucleotide: nt) and three partial sequences (2429nt, 4364nt, and 4419nt in length, including the entire VP1 coding region) of SaV were obtained from one diarrheic and 15 non-diarrheic porcine feces in Japan via a metagenomics approach...
September 18, 2017: Infection, Genetics and Evolution
Brad E Wasserman, Daniel E Carvajal-Hausdorf, Kenneth Ho, Wendy Wong, Natalie Wu, Victor C Chu, Edwin W Lai, Jodi M Weidler, Michael Bates, Veronique Neumeister, David L Rimm
Historically, mRNA measurements have been tested on several commercially available platforms, but none have gained broad acceptance for assessment of HER2. An mRNA measurement, as a continuous value, has the potential for use in adjudication of the equivocal category. Here we use a real-time quantitative reverse transcription polymerase chain reaction (RT-qPCR) assay in a closed, single-use cartridge, automated system. Multiple cores (1 mm in diameter) were retrospectively collected from 80 formalin-fixed paraffin-embedded (FFPE) tissue blocks with invasive breast cancer seen by Yale Pathology Labs between 1998 and 2011...
September 11, 2017: Laboratory Investigation; a Journal of Technical Methods and Pathology
Ismail M Taban, Jinge Zhu, Hector F DeLuca, Claire Simons
A homology model of human CYP27B1 was built using MOE and was further optimised by molecular dynamics simulations of the hCYP27B1 homology model and a hCYP27B1-SDZ-88357 complex. Docking results from the hCYP27B1-SDZ-88357 complex showed amino acids Arg107, Asn387 and Asp320 have an important role in binding interaction, with Asp320 part of the important acid-alcohol pair situated in the I-helix with the conserved sequence (A/G) GX (E/D) (T/S), which assumes an essential role in the binding of an oxygen molecule for catalysis...
August 22, 2017: Bioorganic & Medicinal Chemistry
C C Ding, S Y Wu, L J Zhang, Y Q Xu, Z H Zhang, M H Wu, B H Teng
Making use of the perturbation formulae for 3d(1) ions (Ti(3+) and V(4+) ) under orthorhombically compressed octahedra, the spin Hamiltonian parameters (SHPs) (g factors: gx , gy , gz and hyperfine structure constants: Ax , Ay , Az ) and local structures of the 3d(1) impurity centres C1 , C2 and C3 in KTP crystals are theoretically analyzed in a consistent way. The remarkable local distortions (i.e., the relative axial compression ratios 11.2%, 7.0% and 5.5% along Z axis and the relative planar bond length variation ratios 15...
September 6, 2017: Magnetic Resonance in Chemistry: MRC
Marita G Pereira, Susana Velasco-Lozano, Sonia Moreno-Perez, Aline M Polizeli, Paulo R Heinen, Fernanda D A Facchini, Ana C Vici, Mariana Cereia, Benevides C Pessela, Gloria Fernandez-Lorente, Jose M Guisan, João A Jorge, Maria de Lourdes T M Polizeli
Enzyme immobilization can promote several advantages for their industrial application. In this work, a lipase from Hypocrea pseudokoningii was efficiently linked to four chemical supports: agarose activated with cyanogen bromide (CNBr), glyoxyl-agarose (GX), MANAE-agarose activated with glutaraldehyde (GA) and GA-crosslinked with glutaraldehyde. Results showed a more stable lipase with both the GA-crosslinked and GA derivatives, compared to the control (CNBr), at 50 °C, 60 °C and 70 °C. Moreover, all derivatives were stabilized when incubated with organic solvents at 50%, such as ethanol, methanol, n-propanol and cyclohexane...
September 4, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
Narihiro Shibukawa, Shohei Ouchi, Shuji Wakamatsu, Yuhei Wakahara, Akira Kaneko
AIM: To investigate predictive markers for metachronous and synchronous gastric cancer (GC), which can develop after endoscopic submucosal dissection (ESD). METHODS: A total of 352 patients underwent ESD for early GC at NTT West Osaka Hospital between June 2006 and February 2016. Exclusion criteria were as follows: Remnant stomach, unknown Helicobacter pylori status, and endoscopic observation of the whole stomach outside our hospital. We analyzed data from 192 patients comprising 109 patients with solitary GC (Group A) and 83 with metachronous and synchronous GC (Group B)...
August 15, 2017: World Journal of Gastrointestinal Oncology
Fatmah A S Alasmary, Fatima S Alnahdi, Abir Ben Bacha, Amr M El-Araby, Nadine Moubayed, Ahmed M Alafeefy, Moustafa E El-Araby
Elevated blood glucose and increased activities of secreted phospholipase A2 (sPLA2) are strongly linked to coronary heart disease. In this report, our goal was to develop small heterocyclic compound that inhibit sPLA2. The title compounds were also tested against α-glucosidase and α-amylase. This array of enzymes was selected due to their implication in blood glucose regulation and diabetic cardiovascular complications. Therefore, two distinct series of quinoxalinone derivatives were synthesised; 3-[N'-(substituted-benzylidene)-hydrazino]-1H-quinoxalin-2-ones 3a-f and 1-(substituted-phenyl)-5H-[1,2,4]triazolo[4,3-a]quinoxalin-4-ones 4a-f...
December 2017: Journal of Enzyme Inhibition and Medicinal Chemistry
Albert H Alberts, Gadi Rothenberg
We recount here the story of the discovery and invention of a family of thermoset resins that are fully biodegradable and plant-based. The resin is prepared by polymerising glycerol, the simplest trialcohol, with citric acid, the simplest abundantly available triacid. Mixing these two chemicals at moderate temperatures begins a multi-step esterification that results in a network-like resin foam. The hardness, brittleness, and toughness of this resin can be controlled by changing the alcohol : acid ratio, the temperature, and the presence of additives...
September 21, 2017: Faraday Discussions
Zeng Wang, Huanliang Yang, Yan Chen, Shiyu Tao, Liling Liu, Huihui Kong, Shujie Ma, Fei Meng, Yasuo Suzuki, Chuanling Qiao, Hualan Chen
Efficient transmission from human to human is the prerequisite for an influenza virus to cause a pandemic; however, the molecular determinants of influenza virus transmission are still largely unknown. In this study, we explored the molecular basis for transmission of Eurasian avian-like H1N1 (EAH1N1) swine influenza viruses by comparing two viruses that are genetically similar but differ in their transmissibility in guinea pigs: the A/swine/Guangxi/18/2011 virus (GX/18) is highly transmissible by respiratory droplet in guinea pigs, whereas the A/swine/Heilongjiang/27/2012 virus (HLJ/27) does not transmit in this animal model...
November 1, 2017: Journal of Virology
Lei Jiang, Wenjun Zhao, Zongxi Han, Yuqiu Chen, Yan Zhao, Junfeng Sun, Huixin Li, Yuhao Shao, Liangliang Liu, Shengwang Liu
In 2014, three infectious bronchitis virus (IBV) strains, designated as γCoV/ck/China/I0111/14, γCoV/ck/China/I0114/14 and γCoV/ck/China/I0118/14, were isolated and identified from chickens suspected to be infected with IBV in Guangxi province, China. Based upon data arising from S1 sequence and phylogenetic analyses, the three IBV isolates were genetically different from other known IBV types, which represented a novel genotype (GI-29). Virus cross-neutralization tests, using γCoV/ck/China/I0111/14 as a representative, showed that genotype GI-29 was antigenically different from all other known IBV types, thus representing a novel serotype...
August 8, 2017: Infection, Genetics and Evolution
Rahul Suresh, Rebecca S Blue, Charles Mathers, Tarah L Castleberry, James M Vanderploeg
INTRODUCTION: Hypergravitational exposures during human centrifugation are known to provoke dysrhythmias, including sinus dysrhythmias/tachycardias, premature atrial/ventricular contractions, and even atrial fibrillations or flutter patterns. However, events are generally short-lived and resolve rapidly after cessation of acceleration. This case report describes a prolonged ectopic ventricular rhythm in response to high G exposure. CASE REPORT: A previously healthy 30-yr-old man voluntarily participated in centrifuge trials as a part of a larger study, experiencing a total of 7 centrifuge runs over 48 h...
August 1, 2017: Aerospace Medicine and Human Performance
H Zhang, W Wen, G Hao, Y Hu, H Chen, P Qian, X Li
Atypical porcine pestivirus (APPV) has been considered a novel pestivirus and causative agent of congenital tremor type A-II. An APPV CH-GX2016 strain was characterized from newly born piglets with clinical symptoms of congenital tremor in Guangxi, China. The genome of APPV CH-GX 2016 strain was 11,475 bp in length and encoded a polyprotein composed of the 3,635 amino acids. This genome sequence exhibited 88.0% to 90.8% nucleotide sequence homology with other APPV reference sequences in GenBank. Phylogenetic analysis further showed that APPV CH-GX is a novel pestivirus compared with previously described classical pestivirus strains...
July 14, 2017: Transboundary and Emerging Diseases
Krzysztof Tadyszak, Czesław Rudowicz, Hitoshi Ohta, Takahiro Sakurai
The spin Hamiltonian (SH) parameters experimentally determined by EMR (EPR) may be corroborated or otherwise using various theoretical modeling approaches. To this end semiempirical modeling is carried out for high-spin (S=2) manganese (III) 3d(4) ions in complex of tetraphenylporphyrinato manganese (III) chloride (MnTPPCl). This modeling utilizes the microscopic spin Hamiltonians (MSH) approach developed for the 3d(4) and 3d(6) ions with spin S=2 at orthorhombic and tetragonal symmetry sites in crystals, which exhibit an orbital singlet ground state...
June 28, 2017: Journal of Inorganic Biochemistry
Yibin Qin, Bingxia Lu, Ying He, Bin Li, Qunpeng Duan, Jiaxing Liang, Zhongwei Chen, Qianlian Su, Bingfen Bi, Wu Zhao
We report here the complete genome sequence of porcine epidemic diarrhea virus (PEDV) strain CH/GX/2015/750A (750A), which was isolated from a suckling piglet with watery diarrhea in Guangxi, China. The isolate is genetically close to other recent Chinese variant PEDVs and distinct from the classical PEDVs.
July 6, 2017: Genome Announcements
Rebecca S Blue, Frederick Bonato, Kimberly Seaton, Andrea Bubka, Johnené L Vardiman, Charles Mathers, Tarah L Castleberry, James M Vanderploeg
INTRODUCTION: In commercial spaceflight, anxiety could become mission-impacting, causing negative experiences or endangering the flight itself. We studied layperson response to four varied-length training programs (ranging from 1 h-2 d of preparation) prior to centrifuge simulation of launch and re-entry acceleration profiles expected during suborbital spaceflight. We examined subject task execution, evaluating performance in high-stress conditions. We sought to identify any trends in demographics, hemodynamics, or similar factors in subjects with the highest anxiety or poorest tolerance of the experience...
July 1, 2017: Aerospace Medicine and Human Performance
Thomas U Nick, Kanchana R Ravichandran, JoAnne Stubbe, Müge Kasanmascheff, Marina Bennati
The reaction catalyzed by E. coli ribonucleotide reductase (RNR) composed of α and β subunits that form an active α2β2 complex is a paradigm for proton-coupled electron transfer (PCET) processes in biological transformations. β2 contains the diferric tyrosyl radical (Y122·) cofactor that initiates radical transfer (RT) over 35 Å via a specific pathway of amino acids (Y122· ⇆ [W48] ⇆ Y356 in β2 to Y731 ⇆ Y730 ⇆ C439 in α2). Experimental evidence exists for colinear and orthogonal PCET in α2 and β2, respectively...
July 18, 2017: Biochemistry
Xiao Fang Zhu, Jiang Xue Wan, Qi Wu, Xu Sheng Zhao, Shao Jian Zheng, Ren Fang Shen
Glucuronoxylan (GX), an important component of hemicellulose in the cell wall, appears to affect aluminium (Al) sensitivity in plants. To investigate the role of GX in cell-wall-localized xylan, we examined the Arabidopsis thaliana parvus mutant in detail. This mutant lacks α-D-glucuronic acid (GlcA) side chains in GX and has greater resistance to Al stress than wild-type (WT) plants. The parvus mutant accumulated lower levels of Al in its roots and cell walls than WT despite having cell wall pectin content and pectin methylesterase (PME) activity similar to those of WT...
June 16, 2017: Plant, Cell & Environment
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