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https://www.readbyqxmd.com/read/29413602/molecular-lego-by-domain-imprinting-of-cytochrome-p450-bm3
#1
K J Jetzschmann, A Yarman, L Rustam, P Kielb, V B Urlacher, A Fischer, I M Weidinger, U Wollenberger, F W Scheller
HYPOTHESIS: Electrosynthesis of the MIP nano-film after binding of the separated domains or holo-cytochrome BM3 via an engineered anchor should result in domain-specific cavities in the polymer layer. EXPERIMENTS: Both the two domains and the holo P450 BM3 have been bound prior polymer deposition via a N-terminal engineered his6-anchor to the electrode surface. Each step of MIP preparation was characterized by cyclic voltammetry of the redox-marker ferricyanide...
January 31, 2018: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/29409880/the-effects-of-graded-intraocular-pressure-challenge-on-the-optic-nerve-head
#2
Nimesh Patel, Faith McAllister, Laura Pardon, Ronald Harwerth
Intraocular pressure (IOP) is an important risk factor for glaucoma, and the response of the ONH and surrounding tissues to elevated IOP are often investigated to better understand pathophysiology. In vivo structure including that of the optic nerve head (ONH) and surrounding tissue of the eye are often assessed using optical coherence tomography (OCT). With advances in OCT technology, both large vessels and capillaries can be imaged non-invasively (OCT Angiography). Because a significant portion of retinal thickness is comprised of vasculature, the purpose of the current study was to investigate OCT structural and vascular changes in healthy non-human primate eyes with systematic graded increases and decreases in IOP...
February 1, 2018: Experimental Eye Research
https://www.readbyqxmd.com/read/29386616/bruch-s-membrane-opening-based-optical-coherence-tomography-of-the-optic-nerve-head-a-useful-diagnostic-tool-to-detect-glaucoma-in-macrodiscs
#3
P Enders, F Schaub, W Adler, M M Hermann, T S Dietlein, C Cursiefen, L M Heindl
PurposeTo compare Bruch's membrane opening (BMO)-based spectral domain optical coherence tomography (SD-OCT) and margin based confocal scanning laser tomography (CSLT) of the optic nerve head (ONH) to visual field function in large optic discs (macrodiscs) and to assess performance for glaucoma detection.MethodsIn a case-control, cross-sectional study, 125 eyes of 125 patients with disc size >2.45 mm2, thereof 44 glaucoma and 11 ocular hypertension (OHT) patients and 70 healthy controls underwent SD-OCT and CSLT examination, visual field testing and clinical evaluation...
February 2018: Eye
https://www.readbyqxmd.com/read/29381729/bmo-mir-275-down-regulates-expression-of-bombyx-mori-sericin-gene-2-in-vitro
#4
Ping Qian, Tao Jiang, Xin Wang, Fei Song, Chen Chen, Xingjia Shen
We hypothesized that bmo-miR-275 has a potential regulatory function regarding the expression of sericin gene 2 (BmSer-2). First, we examined the expression of bmo-miR-275 and its target gene BmSer-2 in seven different tissues from 5th instar day-3 silkworm larvae. The results showed that they were both specifically expressed in the middle silk gland, implying that spatio-temporal conditions are required for bmo-miR-275 to regulate the expression of BmSer-2. To test this hypothesis, we constructed a pri-bmo-miR-275 expressing plasmid pcDNA3...
2018: PloS One
https://www.readbyqxmd.com/read/29367818/some-weighted-inequalities-for-hausdorff-operators-and-commutators
#5
Amjad Hussain, Amna Ajaib
In this paper, we consider the problem of boundedness of Hausdorff operator on weighted central Morrey spaces. In particular, we obtain sharp bounds for Hausdorff operators on power weighted central Morrey spaces. Analogous results for the commutators of Hausdorff operators when the symbol functions belong to weighted central-BMO spaces are obtained as well.
2018: Journal of Inequalities and Applications
https://www.readbyqxmd.com/read/29342153/the-impact-of-optic-nerve-and-related-characteristics-on-disc-area-measurements-derived-from-different-imaging-techniques
#6
Michael Yapp, George Rennie, Michael P Hennessy, Michael Kalloniatis, Barbara Zangerl
PURPOSE: Optic nerve head (ONH) assessment and its interpretation in healthy patients and those with glaucoma remains a pivotal topic specifically considering rapid advancements in imaging technologies. We undertook a large-scale, mixed cohort, comparative study to assess the correlation of optic disc measurements between different imaging modalities and investigated the impact of patient and disc associated parameters. METHODS: ONH sizes were obtained from one randomly selected eye of each of 209 patients using stereophotography, confocal scanning laser ophthalmoscopy and two different optical coherence tomographers (OCT)...
2018: PloS One
https://www.readbyqxmd.com/read/29332249/correlation-of-flow-density-as-measured-using-optical-coherence-tomography-angiography-with-structural-and-functional-parameters-in-glaucoma-patients
#7
Maged Alnawaiseh, Larissa Lahme, Viktoria Müller, André Rosentreter, Nicole Eter
PURPOSE: To evaluate the correlation between flow density, as measured by optical coherence tomography angiography (OCTA), and structural and functional parameters in patients with open-angle glaucoma. METHODS: Thirty-four eyes of 34 patients with open-angle glaucoma and 35 eyes of 35 healthy subjects were prospectively included in this study. OCTA was performed using RTVue XR Avanti with AngioVue. The macula was imaged with a 3 × 3 mm scan and the optic nerve head (ONH) with a 4...
January 13, 2018: Graefe's Archive for Clinical and Experimental Ophthalmology
https://www.readbyqxmd.com/read/29329141/precision-of-optic-nerve-head-and-retinal-nerve-fiber-layer-parameter-measurements-by-spectral-domain-optical-coherence-tomography
#8
Laura M Schrems-Hoesl, Wolfgang A Schrems, Robert Laemmer, Friedrich E Kruse, Christian Y Mardin
PURPOSE: The aim of this study was to assess the repeatability and reproducibility (R&R) of Bruch's Membrane Opening - based on Minimum Rim Width (BMO-MRW), Minimum Rim Area (BMO-MRA) and peripapillary retinal nerve fiber layer thickness (RNFLT) with the Spectralis OCT (Heidelberg Engineering) for normal and glaucoma subjects. Precise measurement of these parameters can support detection of structural glaucomatous damage and progression. METHODS: This cross-sectional study included 16 healthy controls and 16 patients with glaucoma...
January 11, 2018: Journal of Glaucoma
https://www.readbyqxmd.com/read/29327305/five-year-postoperative-outcomes-of-modified-staged-canal-wall-up-tympanoplasty-for-primary-acquired-cholesteatoma
#9
Masahiro Komori, Naoaki Yanagihara, Jun Hyodo, Ryosei Minoda, Yasuyuki Hinohira
OBJECTIVE: For successful canal wall up tympanoplasty (CWUT) for the treatment of cholesteatoma, the restoration of stable middle ear aeration is also important; however, little is known about the dynamics of such aeration or the optimal surgical procedure. In this study, alternative additional surgical procedure was selected based on the grade of middle ear aeration during the second-stage operation. SUBJECTS AND METHODS: Patients included in this study underwent staged CWUT surgeries with mastoid cortex plasty (MCP) for well-aerated ears (grade 3) and bony mastoid obliteration (BMO) for poorly aerated ears (grade 2-0)...
January 11, 2018: European Archives of Oto-rhino-laryngology
https://www.readbyqxmd.com/read/29327060/optical-coherence-tomography-analysis-of-the-optic-nerve-head-and-surrounding-structures-in-long-duration-international-space-station-astronauts
#10
Nimesh Patel, Anastas Pass, Sara Mason, Charles R Gibson, Christian Otto
Importance: After long-duration spaceflight, morphological changes in the optic nerve head (ONH) and surrounding tissues have been reported. Objective: To develop methods to quantify ONH and surrounding tissue changes using preflight and postflight optical coherence tomographic scans of the ONH region. Design, Setting, and Participants: Two separate analyses were done on retrospective data, with the first comparing a preflight group with a control group, followed by preflight to postflight analysis...
January 11, 2018: JAMA Ophthalmology
https://www.readbyqxmd.com/read/29305731/association-of-bruch-s-membrane-opening-and-optic-disc-morphology-to-axial-length-and-visual-field-defects-in-eyes-with-primary-open-angle-glaucoma
#11
Hideo Nakanishi, Kenji Suda, Munemitsu Yoshikawa, Tadamichi Akagi, Takanori Kameda, Hanako Ohashi Ikeda, Satoshi Yokota, Yasuo Kurimoto, Akitaka Tsujikawa
PURPOSE: To examine the morphology of Bruch's membrane opening (BMO), optic disc, and peripapillary atrophy (PPA) by scanning laser ophthalmoscopy (SLO) and spectral-domain optical coherence tomography (SD-OCT), and to determine their association with the axial length and visual field defects. METHODS: This was a cross-sectional study of 94 eyes of 56 subjects; 77 eyes were diagnosed with primary open-angle glaucoma and 17 eyes as normal. The margins of the optic disc were determined in the SLO images, and that of the BMO in the SD-OCT images...
January 5, 2018: Graefe's Archive for Clinical and Experimental Ophthalmology
https://www.readbyqxmd.com/read/29230422/novel-semiquantitative-bone-marrow-oedema-score-and-fracture-score-for-the-magnetic-resonance-imaging-assessment-of-the-active-charcot-foot-in-diabetes
#12
L Meacock, N L Petrova, Ana Donaldson, A Isaac, A Briody, R Ramnarine, M E Edmonds, D A Elias
There are no accepted methods to grade bone marrow oedema (BMO) and fracture on magnetic resonance imaging (MRI) scans in Charcot osteoarthropathy. The aim was to devise semiquantitative BMO and fracture scores on foot and ankle MRI scans in diabetic patients with active osteoarthropathy and to assess the agreement in using these scores. Three radiologists assessed 45 scans (Siemens Avanto 1.5T, dedicated foot and ankle coil) and scored independently twenty-two bones (proximal phalanges, medial and lateral sesamoids, metatarsals, tarsals, distal tibial plafond, and medial and lateral malleoli) for BMO (0-no oedema, 1-oedema < 50% of bone volume, and 2-oedema > 50% of bone volume) and fracture (0-no fracture, 1-fracture, and 2-collapse/fragmentation)...
2017: Journal of Diabetes Research
https://www.readbyqxmd.com/read/29203329/bruch-s-membrane-opening-on-optical-coherence-tomography-in-pediatric-papilledema-and-pseudopapilledema
#13
Atalie C Thompson, M Tariq Bhatti, Mays A El-Dairi
PURPOSE: To determine whether the diameter of Bruch's membrane opening (BMO) can distinguish mild papilledema from pseudopapilledema using optical coherence tomography (OCT). METHODS: The medical records of pediatric patients with pseudopapilledma due to optic nerve head (ONH) drusen, patients with papilledema, and normal control subjects were retrospectively reviewed. All eyes underwent OCT imaging of the BMO and retinal nerve fiber layer (RNFL). Transverse horizontal diameter of the BMO and papillary height were measured...
December 1, 2017: Journal of AAPOS: the Official Publication of the American Association for Pediatric Ophthalmology and Strabismus
https://www.readbyqxmd.com/read/29177702/-bruch-s-membrane-opening-minimum-rim-width-correlation-and-diagnostic-accuracy-in-comparison-to-peripapillary-retinal-nerve-fiber-layer-thickness
#14
M Awe, S Khalili-Amiri, I R Volkmann, B Junker, C Framme, K Hufendiek
BACKGROUND: Glaucoma is one of the main causes of blindness in the Western hemisphere. Because the disease often painlessly progresses it remains unnoticed until major optic nerve head damage occurs in many cases. That is why new, more sensitive diagnostic methods are needed. Bruch's membrane opening minimum rim width (BMO-MRW), measured with the new glaucoma module premium edition (GMPE) was recently introduced as a more accurate tool to detect glaucomatous changes. The purpose of this study was to assess the correlation of SPECTRALIS® spectral domain optical coherence tomography (SD-OCT) and the GMPE anatomic positioning module (APS module) for retinal nerve fiber layer thickness (RNFLT) measurements...
November 24, 2017: Der Ophthalmologe: Zeitschrift der Deutschen Ophthalmologischen Gesellschaft
https://www.readbyqxmd.com/read/29157363/detecting-structural-progression-in-glaucoma-with-optical-coherence-tomography
#15
REVIEW
Andrew J Tatham, Felipe A Medeiros
Optical coherence tomography (OCT) is increasingly used to obtain objective measurements of the retinal nerve fiber layer (RNFL), optic nerve head, and macula for assessing glaucoma progression. Although OCT has been adopted widely in clinical practice, uncertainty remains concerning its optimal role. Questions include: What is the best structure to measure? What quantity of change is significant? Are structural changes relevant to the patient? How are longitudinal measurements affected by aging? How can changes resulting from aging be differentiated from true progression? How best should OCT be used alongside visual fields, and how often should OCT be performed? Recent studies have addressed some of these questions...
December 2017: Ophthalmology
https://www.readbyqxmd.com/read/29119022/bruch-s-membrane-opening-minimum-rim-width-measurement-with-sd-oct-a-method-to-correct-for-the-opening-size-of-bruch-s-membrane
#16
Robert Kromer, Martin Stephan Spitzer
A precise evaluation of the retinal nerve fiber layer thickness (RNFLT) is key for diagnosing and monitoring glaucoma. The Bruch's membrane opening minimum rim width (BMO-MRW) has been proposed as a reproducible assessment of the optic nerve. The BMO-MRW measures the minimum distance from the BMO to the internal limiting membrane. We propose an approach to correct the BMO-MRW using the BMO size for increased accuracy in interindividual comparisons in future studies. Eighty-one healthy patients received SPECTRALIS spectral domain optical coherence tomography measurements for the peripapillary RNFLT and BMO-MRW...
2017: Journal of Ophthalmology
https://www.readbyqxmd.com/read/29080488/synergistic-effects-of-biogenic-manganese-oxide-and-mn-ii-oxidizing-bacterium-pseudomonas-putida-strain-mnb1-on-the-degradation-of-17-%C3%AE-ethinylestradiol
#17
Thi Nhung Tran, Do-Gun Kim, Seok-Oh Ko
While biogenic manganese oxide (BMO) generated via the oxidation of Mn(II) by the Mn-oxidizing bacteria (MOB) have received attention, the relative roles of biological activity by MOB themselves were not clearly investigated. In this study, the synergistic effects of BMO and MOB Pseudomonas putida strain MnB1 on the degradation of 17α-ethinylestradiol (EE2) was investigated. Experiments with BMO in the presence of P. putida MnB1 showed 15-fold higher removal than that with BMO alone, suggesting that EE2 degradation was mediated by the biological activity of MOB as well as abiotic reaction by BMO...
October 23, 2017: Journal of Hazardous Materials
https://www.readbyqxmd.com/read/29066838/optimization-strategies-for-bruch-s-membrane-opening-minimum-rim-area-calculation-sequential-versus-simultaneous-minimization
#18
Philip Enders, Werner Adler, Friederike Schaub, Manuel M Hermann, Michael Diestelhorst, Thomas Dietlein, Claus Cursiefen, Ludwig M Heindl
To compare a simultaneously optimized continuous minimum rim surface parameter between Bruch's membrane opening (BMO) and the internal limiting membrane to the standard sequential minimization used for calculating the BMO minimum rim area in spectral domain optical coherence tomography (SD-OCT). In this case-control, cross-sectional study, 704 eyes of 445 participants underwent SD-OCT of the optic nerve head (ONH), visual field testing, and clinical examination. Globally and clock-hour sector-wise optimized BMO-based minimum rim area was calculated independently...
October 24, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29062772/structure-function-relationship-comparison-between-retinal-nerve-fibre-layer-and-bruch-s-membrane-opening-minimum-rim-width-in-glaucoma
#19
Lukas Reznicek, Sophie Burzer, Andrea Laubichler, Ali Nasseri, Chris P Lohmann, Nikolaus Feucht, Michael Ulbig, Mathias Maier
AIM: To evaluate and compare structural optical coherence tomography (OCT)-based parameters, such as Bruch's membrane opening-minimum rim width (BMO-MRW), and retinal nerve fiber layer (RNFL) thickness in glaucoma patients with visual field (VF) defects, and to correlate both to mean deviation (MD) values of obtained standard achromatic perimetry (SAP) examinations. METHODS: Patients with glaucoma and glaucomatous VF defects were enrolled in this prospective study and compared to age-matched healthy individuals...
2017: International Journal of Ophthalmology
https://www.readbyqxmd.com/read/29049719/intraday-repeatability-of-bruch-s-membrane-opening-based-neuroretinal-rim-measurements
#20
Philip Enders, Andreas Bremen, Friederike Schaub, Manuel M Hermann, Michael Diestelhorst, Thomas Dietlein, Claus Cursiefen, Ludwig M Heindl
Purpose: To assess possible intraday variability in Bruch's membrane opening-based (BMO) assessment of neuroretinal rim by spectral-domain optical coherence tomography (SD-OCT) of the optic nerve head (ONH) as well as to evaluate its independence from intraindividual IOP changes. Methods: In this noninterventional, prospective cohort study, 51 consecutively enrolled patients with glaucoma or ocular hypertension underwent standardized SD-OCT of the ONH and IOP assessment at two different time points with a time gap of 5 hours on the same day...
October 1, 2017: Investigative Ophthalmology & Visual Science
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