keyword
MENU ▼
Read by QxMD icon Read
search

Bone ablation

keyword
https://www.readbyqxmd.com/read/28634334/image-guided-transplantation-of-single-cells-in-the-bone-marrow-of-live-animals
#1
Raphaël Turcotte, Clemens Alt, Judith M Runnels, Kyoko Ito, Juwell W Wu, Walid Zaher, Luke J Mortensen, Lev Silberstein, Daniel C Côté, Andrew L Kung, Keisuke Ito, Charles P Lin
Transplantation of a single hematopoietic stem cell is an important method for its functional characterization, but the standard transplantation protocol relies on cell homing to the bone marrow after intravenous injection. Here, we present a method to transplant single cells directly into the bone marrow of live mice. We developed an optical platform that integrates a multiphoton microscope with a laser ablation unit for microsurgery and an optical tweezer for cell micromanipulation. These tools allow image-guided single cell transplantation with high spatial control...
June 20, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28620244/specific-ablation-of-mouse-fam20c-in-cells-expressing-type-i-collagen-leads-to-skeletal-defects-and-hypophosphatemia
#2
Peihong Liu, Su Ma, Hua Zhang, Chao Liu, Yongbo Lu, Li Chen, Chunlin Qin
FAM20C mutations in humans cause Raine syndrome and our previous studies showed that global inactivation of mouse Fam20C led to bone and dental defects. By crossbreeding 2.3  kb Col 1a1-Cre mice with Fam20C (flox/flox) mice, we created 2.3  kb Col 1a1-Cre;Fam20C (foxl/flox) (cKO) mice, in which Fam20C was inactivated in cells expressing Type I collagen. This study showed that the long bones of cKO mice were shorter and had a lower level of mineralization compared to the normal mice. The collagen fibrils in Fam20C-deficient bone were disorganized and thicker while the growth plate cartilage in cKO mice was disorganized and wider compared to the normal mice...
June 15, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28618241/use-of-adjuvant-bisphosphonates-and-other-bone-modifying-agents-in-breast-cancer-a-cancer-care-ontario-and-american-society-of-clinical-oncology-clinical-practice-guideline
#3
Sukhbinder Dhesy-Thind, Glenn G Fletcher, Phillip S Blanchette, Mark J Clemons, Melissa S Dillmon, Elizabeth S Frank, Sonal Gandhi, Rasna Gupta, Mihaela Mates, Beverly Moy, Ted Vandenberg, Catherine H Van Poznak
Purpose To make recommendations regarding the use of bisphosphonates and other bone-modifying agents as adjuvant therapy for patients with breast cancer. Methods Cancer Care Ontario and ASCO convened a Working Group and Expert Panel to develop evidence-based recommendations informed by a systematic review of the literature. Results Adjuvant bisphosphonates were found to reduce bone recurrence and improve survival in postmenopausal patients with nonmetastatic breast cancer. In this guideline, postmenopausal includes patients with natural menopause or that induced by ovarian suppression or ablation...
June 20, 2017: Journal of Clinical Oncology: Official Journal of the American Society of Clinical Oncology
https://www.readbyqxmd.com/read/28615414/loss-of-ip3-receptor-mediated-ca-2-release-in-mouse-b-cells-results-in-abnormal-b-cell-development-and-function
#4
Huayuan Tang, Hong Wang, Qingsong Lin, Feifei Fan, Fei Zhang, Xiaohong Peng, Xi Fang, Jie Liu, Kunfu Ouyang
Intracellular calcium (Ca(2+)) mobilization after engagement of the BCR has been proposed to play an important role in B cell development and function. BCR activation causes an initial Ca(2+) release from the endoplasmic reticulum that is mediated by inositol 1,4,5-trisphosphate receptor (IP3R) and then triggers store-operated Ca(2+) entry once endoplasmic reticulum Ca(2+) store is depleted. Store-operated Ca(2+) entry has been shown to regulate B cell function but is dispensable for B cell development. By contrast, the function of IP3R-mediated Ca(2+) release in B cells remains to be determined...
June 14, 2017: Journal of Immunology: Official Journal of the American Association of Immunologists
https://www.readbyqxmd.com/read/28597707/ct-guided-radiofrequency-ablation-of-spinal-osteoblastoma-treatment-and-long-term-follow-up
#5
Francesco Arrigoni, Antonio Barile, Luigi Zugaro, Eva Fascetti, Marcello Zappia, Luca Brunese, Carlo Masciocchi
OBJECTIVE: Osteoblastoma (OB) is a painful, rare, benign bone tumour usually observed in young populations, and this condition involves the spine in up to one-third of cases. We sought to focus on the minimally invasive treatment of spinal OB with radiofrequency ablation (RFA) under computed tomography (CT) guidance. When performed near the spinal cord, surgery can lead to instability of the spine, sometimes requiring additional interventions to stabilise the segments involved, and can cause the precocious onset of arthrosis or other degenerative diseases...
June 9, 2017: International Journal of Hyperthermia
https://www.readbyqxmd.com/read/28597402/metastatic-bone-disease-from-breast-cancer-a-review-of-minimally-invasive-techniques-for-diagnosis-and-treatment
#6
REVIEW
Dimitrios Filippiadis, Andreas F Mavrogenis, Argyro Mazioti, Konstantinos Palialexis, Panayiotis D Megaloikonomos, Panayiotis J Papagelopoulos, Alexis Kelekis
Skeletal-related events in patients with metastatic bone disease include intractable severe pain, pathologic fracture, spinal cord and nerve compression, hypercalcemia and bone marrow aplasia. In patients with breast cancer, the skeleton is the most frequent site for metastases. Treatment options for metastatic bone disease in these patients include bisphosphonates, chemotherapeutic agents, opioids, hormonal therapy, minimally invasive/interventional and surgical techniques. Interventional oncology techniques for breast cancer patients with bone metastases include diagnostic (biopsy) and therapeutic (palliative and curative) approaches...
June 8, 2017: European Journal of Orthopaedic Surgery & Traumatology: Orthopédie Traumatologie
https://www.readbyqxmd.com/read/28596553/hematopoietic-id-deletion-triggers-endomyocardial-fibrotic-and-vascular-defects-in-the-adult-heart
#7
Corey Chang, Qingshi Zhao, J Patrick Gonzalez, Jung H Kim, Kamal Alzahrani, Dominic Del Re, Diego Fraidenraich
Inhibitor of DNA binding (Id) proteins play important roles in regulating cardiac development via paracrine signaling. Id1/Id3 knockout mice die at mid-gestation with multiple cardiac defects. Single Id knockout studies have not reported cardiomyopathies. To bypass embryonic lethality we used Tie2CRE-mediated recombination to conditionally delete Id1 against global Id3 ablation (Id cDKOs), which develops adult-onset dilated cardiomyopathy. We confirm upregulation of thrombospondin-1 (TSP1) in Id cDKO hearts...
June 8, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28589100/microrna-21-limits-uptake-of-listeria-monocytogenes-by-macrophages-to-reduce-the-intracellular-niche-and-control-infection
#8
Daniel G W Johnston, Jay Kearney, Zbigniew Zasłona, Michelle A Williams, Luke A J O'Neill, Sinéad C Corr
MiRNAs are important post-transcriptional regulators of gene expression. MiRNA expression is a crucial part of host responses to bacterial infection, however there is limited knowledge of their impact on the outcome of infections. We investigated the influence of miR-21 on macrophage responses during infection with Listeria monocytogenes, which establishes an intracellular niche within macrophages. MiR-21 is induced following infection of bone marrow-derived macrophages (BMDMs) with Listeria. MiR-21(-/-) macrophages display an increased bacterial burden with Listeria at 30 min and 2 h post-infection...
2017: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/28585695/the-rhogap-myo9b-promotes-bone-growth-by-mediating-osteoblastic-responsiveness-to-igf-1
#9
Brooke K McMichael, Yong-Hoon Jeong, Justin A Auerbach, Cheol-Min Han, Ryan Sedlar, Vikram Shettigar, Martin Bähler, Sudha Agarwal, Do-Gyoon Kim, Beth S Lee
The RhoA subfamily of Rho GTPases regulates actin-based cellular functions in bone such as differentiation, migration, and mechanotransduction. Polymorphisms or genetic ablation of RHOA and some of its regulatory guanine exchange factors (GEFs) have been linked to poor bone health in humans and mice, but the effects of RhoA-specific GTPase-activating proteins (GAPs) on bone quality have not yet been identified. Therefore, we examined the consequences of RhoGAP Myo9b gene knockout on bone growth, phenotype and cellular activity...
June 6, 2017: Journal of Bone and Mineral Research: the Official Journal of the American Society for Bone and Mineral Research
https://www.readbyqxmd.com/read/28585302/rictor-mtorc2-promotes-macrophage-activation-and-kidney-fibrosis
#10
Jiafa Ren, Jianzhong Li, Ye Feng, Bingyan Shu, Yuan Gui, Wei Wei, Weichun He, Junwei Yang, Chunsun Dai
Mammalian target of rapamycin (mTOR) signaling controls many essential cellular functions. However, the role for Rictor/mTORC2 in regulating macrophage activation and kidney fibrosis remains largely unknown. We report here that Rictor/mTORC2 was activated in macrophages from the fibrotic kidneys of mice. Ablation of Rictor in macrophages diminished kidney fibrosis, inflammatory cell accumulation, macrophage proliferation and polarization after unilateral ureter obstruction (UUO) or ischemia/reperfusion injury (IRI)...
June 6, 2017: Journal of Pathology
https://www.readbyqxmd.com/read/28581352/interferon-%C3%AE-mediates-the-immunosuppression-of-bone-marrow-mesenchymal-stem-cells-on-t-lymphocytes-in-vitro
#11
Chen Liang, Erlie Jiang, Jianfeng Yao, Mei Wang, Shulian Chen, Zheng Zhou, Weihua Zhai, Qiaoling Ma, Sizhou Feng, Mingzhe Han
OBJECTIVES: In the present study, we first confirmed the suppressive function of MSCs in allogeneic T cell proliferation and then examined the underlying mechanisms for MSCs' immunomodulation and the role of the pro-inflammatory cytokine interferon (IFN)-γ. METHODS: Human MSCs were cultured in the presence or absence of IFN-γ. The expression level of prostaglandin E2 (PGE2), hepatocyte growth factor (HGF), transforming growth factor (TGF)-β1 and indoleamine 2,3-dioxygenase (IDO) by MSCs were measured...
June 5, 2017: Hematology (Amsterdam, Netherlands)
https://www.readbyqxmd.com/read/28579680/decision-making-osteoplasty-ablation-or-combined-therapy-for-spinal-metastases
#12
REVIEW
William Lea, Sean Tutton
Metastatic disease involving the spine and pelvis is common, often resulting in significant pain and disability. Several percutaneous interventions have been described, including osteoplasty, ablation, and screw fixation, that when used alone or in combination can significantly reduce pain and disability from metastatic bone disease. Although it is possible to make a significant impact in patient care with basic principles and techniques, certain advanced techniques can extend the application of percutaneous interventions while minimizing morbidity...
June 2017: Seminars in Interventional Radiology
https://www.readbyqxmd.com/read/28573314/reduced-toxicity-myeloablative-hla-haploidentical-hematopoietic-stem-cell-transplantation-with-post-transplantation-cyclophosphamide-for-sickle-cell-disease
#13
Volker Wiebking, Sebastian Hütker, Irene Schmid, Stefanie Immler, Tobias Feuchtinger, Michael H Albert
Allogeneic hematopoietic stem cell transplantation (HSCT) offers the possibility of cure for sickle cell disease (SCD) patients. Unfortunately, the probability of finding an HLA-matched donor for SCD patients is low. HSCT from HLA-haploidentical donors using reduced intensity conditioning, unmanipulated bone marrow and post-transplantation cyclophosphamide (ptCy) has resulted in negligible toxicity but high rates of graft rejection. We hypothesized that combining ptCy with a myeloablative reduced toxicity conditioning including serotherapy to increase immune ablation would allow for better engraftment...
June 1, 2017: Annals of Hematology
https://www.readbyqxmd.com/read/28551764/autofluorescence-spectroscopy-for-nerve-sparing-laser-surgery-of-the-head-and-neck-the-influence-of-laser-tissue-interaction
#14
Florian Stelzle, Maximilian Rohde, Max Riemann, Nicolai Oetter, Werner Adler, Katja Tangermann-Gerk, Michael Schmidt, Christian Knipfer
The use of remote optical feedback systems represents a promising approach for minimally invasive, nerve-sparing laser surgery. Autofluorescence properties can be exploited for a fast, robust identification of nervous tissue. With regard to the crucial step towards clinical application, the impact of laser ablation on optical properties in the vicinity of structures of the head and neck has not been investigated up to now. We acquired 24,298 autofluorescence spectra from 135 tissue samples (nine ex vivo tissue types from 15 bisected pig heads) both before and after ER:YAG laser ablation...
May 27, 2017: Lasers in Medical Science
https://www.readbyqxmd.com/read/28546545/extensive-phenotypic-characterization-of-a-new-transgenic-mouse-reveals-pleiotropic-perturbations-in-physiology-due-to-mesenchymal-hgh-minigene-expression
#15
Aimilios Kaklamanos, Jan Rozman, Manolis Roulis, Niki Karagianni, Maria Armaka, Moya Wu, Laura Brachthäuser, Julia Calzada-Wack, Marion Horsch, Johannes Beckers, Birgit Rathkolb, Thure Adler, Frauke Neff, Eckhard Wolf, Valerie Gailus-Durner, Helmut Fuchs, Martin Hrabe de Angelis, George Kollias
The human growth hormone (hGH) minigene used for transgene stabilization in mice has been recently identified to be locally expressed in the tissues where transgenes are active and associated with phenotypic alterations. Here we extend these findings by analyzing the effect of the hGH minigene in TgC6hp55 transgenic mice which express the human TNFR1 under the control of the mesenchymal cell-specific CollagenVI promoter. These mice displayed a fully penetrant phenotype characterized by growth enhancement accompanied by perturbations in metabolic, skeletal, histological and other physiological parameters...
May 25, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28542246/a-drastic-superoxide-dependent-oxidative-stress-is-prerequisite-for-the-down-regulation-of-irp1-insights-from-studies-on-sod1-deficient-mice-and-macrophages-treated-with-paraquat
#16
Anna Milczarek, Rafał R Starzyński, Agnieszka Styś, Aneta Jończy, Robert Staroń, Agnieszka Grzelak, Paweł Lipiński
Iron regulatory protein 1 (IRP1) is a cytosolic bifunctional [4Fe-4S] protein which exhibits aconitase activity or binds iron responsive elements (IREs) in untranslated regions of specific mRNA encoding proteins involved in cellular iron metabolism. Superoxide radical (O2.-) converts IRP1 from a [4Fe-4S] aconitase to a [3Fe-4S] "null" form possessing neither aconitase nor trans-regulatory activity. Genetic ablation of superoxide dismutase 1 (SOD1), an antioxidant enzyme that acts to reduce O2.- concentration, revealed a new O2...
2017: PloS One
https://www.readbyqxmd.com/read/28540809/percutaneous-ct-guided-radiofrequency-ablation-for-patients-with-extrahepatic-oligometastases-of-hepatocellular-carcinoma-long-term-results
#17
Luwen Mu, Lin Sun, Tao Pan, Ning Lyu, Shaolong Li, Xishan Li, Jianpeng Wang, Qiankun Xie, Haijing Deng, Lie Zheng, Jianhong Peng, Lujun Shen, Weijun Fan, Peihong Wu, Ming Zhao
BACKGROUND: The aim of this study was to evaluate the therapeutic outcome of percutaneous computed tomography (CT)-guided radiofrequency ablation (RFA) for extrahepatic oligometastases of hepatocellular carcinoma (HCC). METHODS: Institutional review board approval was obtained for this retrospective study, and all patients provided written informed consent. Between April 2004 and December 2015, 116 oligometastases (diameter, 5-50 mm; 20.3 ± 10.4) in 79 consecutive HCC patients (73 men and 6 women; average age, 50...
May 2, 2017: International Journal of Hyperthermia
https://www.readbyqxmd.com/read/28540807/technical-aspects-of-osteoid-osteoma-ablation-in-children-using-mr-guided-high-intensity-focussed-ultrasound
#18
Pavel S Yarmolenko, Avinash Eranki, Ari Partanen, Haydar Celik, AeRang Kim, Matthew Oetgen, Viktoriya Beskin, Domiciano Santos, Janish Patel, Peter C W Kim, Karun Sharma
BACKGROUND: Osteoid osteoma (OO) is a painful bone tumour occurring in children and young adults. Magnetic resonance imaging-guided high intensity focussed ultrasound (MR-HIFU) allows non-invasive treatment without ionising radiation exposure, in contrast to the current standard of care treatment with radiofrequency ablation (RFA). This report describes technical aspects of MR-HIFU ablation in the first 8 paediatric OO patients treated in a safety and feasibility clinical trial (total enrolment of up to 12 patients)...
April 24, 2017: International Journal of Hyperthermia
https://www.readbyqxmd.com/read/28540776/computed-tomography-guided-radiofrequency-ablation-of-intra-articular-osteoid-osteoma-a-single-centre-s-experience
#19
D K Filippiadis, G Velonakis, C Kostantos, V Kouloulias, E Brountzos, N Kelekis, A Kelekis
OBJECTIVE: To evaluate safety and efficacy of radiofrequency ablation (RFA) in the treatment of painful intra-articular osteoid osteoma. MATERIALS AND METHODS: During the last 3 years, 15 patients underwent computed tomography (CT)-guided biopsy and RFA of symptomatic intra-articular osteoid osteoma. In order to assess and sample the nidus, a coaxial bone biopsy system was used. Biopsy was performed and followed by ablation session with osteoid osteoma protocol in all cases...
March 1, 2017: International Journal of Hyperthermia
https://www.readbyqxmd.com/read/28532400/oligometastases-from-prostate-cancer-local-treatment-with-stereotactic-body-radiotherapy-sbrt
#20
Gregor Habl, Christoph Straube, Kilian Schiller, Marciana Nona Duma, Markus Oechsner, Kerstin A Kessel, Matthias Eiber, Markus Schwaiger, Hubert Kübler, Jürgen E Gschwend, Stephanie E Combs
BACKGROUND: The impact of local tumor ablative therapy in oligometastasized prostate cancer (PC) is still under debate. To gain data for this approach, we evaluated oligometastasized PC patients receiving stereotactic body radiotherapy (SBRT) to bone metastases. METHODS: In this retrospective study, 15 oligometastasized PC patients with a total of 20 bone metastases were evaluated regarding biochemical progression-free survival (PSA-PFS), time to initiation of ADT, and local control rate (LCR)...
May 22, 2017: BMC Cancer
keyword
keyword
37116
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"