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https://www.readbyqxmd.com/read/28641205/muscilage-characterization-biochemical-and-enzymatic-activities-of-laser-irradiated-lagenaria-siceraria-seedlings
#1
Mazhar Abbas, Muhammad Arshad, Numrah Nisar, Jan Nisar, Abdul Ghaffar, Arif Nazir, M Asif Tahir, Munawar Iqbal
Laser stimulation effect on L. siceraria seed mucilage, biochemicals and enzymatic activities during early growth stages were investigated. The laser density power of 1mW/cm(2) for 3 and 5min treatments were performed and various responses i.e., seedlings mucilage, biochemical and enzymatic activities were studied. Laser treatment of L. siceraria seeds enhanced the biochemical as well as the enzymatic activities. TPC (total phenolic contents),TFC (total flavonoids contents), TSS (total soluble sugar), reducing sugar, proline contents, total soluble protein and nitrogen contents were recorded higher in laser treated groups versus control...
June 10, 2017: Journal of Photochemistry and Photobiology. B, Biology
https://www.readbyqxmd.com/read/28641075/postconditioning-with-intralipid-emulsion-protects-against-reperfusion-injury-in-postinfarct-remodeled-rat-hearts-by-activation-of-ros-akt-erk-signaling
#2
Michael Zaugg, Phing-How Lou, Eliana Lucchinetti, Manoj Gandhi, Alexander S Clanachan
The clinically used lipid emulsion Intralipid (ILE) reduces ischemia reperfusion injury in healthy rodent hearts. We tested whether ILE is cardioprotective in postinfarct remodeled hearts. Postinfarct remodeled and sham Sprague-Dawley rat hearts were perfused in working mode and subjected to ischemia (15 minutes) and reperfusion (30 minutes). Left ventricular (LV) work was measured in hearts that were untreated or that received ILE (1%) postconditioning administered at the onset of reperfusion, or the reactive oxygen species (ROS) scavenger N-(2-mercaptopropionyl)-glycine (10 μM) alone or in combination with ILE...
June 1, 2017: Translational Research: the Journal of Laboratory and Clinical Medicine
https://www.readbyqxmd.com/read/28640975/nac-transcription-factor-jungbrunnen1-enhances-drought-tolerance-in-tomato
#3
Venkatesh P Thirumalaikumar, Vikas Devkar, Nikolay Mehterov, Shawkat Ali, Rengin Ozgur, Ismail Turkan, Bernd Mueller-Roeber, Salma Balazadeh
Water deficit (drought stress) massively restricts plant growth and the yield of crops; reducing the deleterious effects of drought is therefore of high agricultural relevance. Drought triggers diverse cellular processes including the inhibition of photosynthesis, the accumulation of cell-damaging reactive oxygen species, and gene expression reprogramming, besides others. Transcription factors (TF) are central regulators of transcriptional reprogramming and expression of many TF genes is affected by drought, including members of the NAC family...
June 22, 2017: Plant Biotechnology Journal
https://www.readbyqxmd.com/read/28640632/gut-microbiota-nitric-oxide-and-microglia-as-pre-requisites-for-neurodegenerative-disorders
#4
Joyce Ka Yu Tse
Regulating fluctuating endogenous nitric oxide (NO) levels is necessary for proper physiological functions. Aberrant NO pathways are implicated in a number of neurological disorders, including Alzheimer's Disease (AD) and Parkinson's Disease. The mechanism of NO in oxidative and nitrosative stress with pathological consequences involves reactions with reactive oxygen species (e.g. superoxide) to form the highly reactive peroxynitrite, hydrogen peroxide, hypochloride ions and hydroxyl radical. NO levels are typically regulated by endogenous nitric oxide synthases (NOS), and inflammatory iNOS is implicated in the pathogenesis of neurodegenerative diseases, in which elevated NO mediates axonal degeneration and activates cyclooxygenases to provoke neuroinflammation...
June 22, 2017: ACS Chemical Neuroscience
https://www.readbyqxmd.com/read/28640594/biomedical-diagnostics-enabled-by-integrated-organic-and-printed-electronics
#5
Termeh Ahmadraji, Laura Gonzalez-Macia, Tapio Ritvonen, Andreas Willert, Saara Tuurala, Satu Ylimaula, David Donaghy, Mika Suhonen, Dave Smart, Vitaly Efremov, Aoife Morrin, Reinhard R Baumann, Munira Raja, Antti Kemppainen, Anthony J Killard
Organic and printed electronics integration has the potential to revolutionise many technologies, including biomedical diag-nostics. This work demonstrates the successful integration of multiple printed electronic functionalities into a single device capable of the measurement of hydrogen peroxide, and total cholesterol. The single-use device employed printed electrochemical sen-sors for hydrogen peroxide electroreduction integrated with printed electrochromic display and battery. The system was driven by a conventional electronic circuit designed to illustrate the complete integration of silicon ICs via pick and place, or using organic electronic circuits...
June 22, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28637348/hydrogen-peroxide-sensing-based-on-inner-surfaces-modification-of-solid-state-nanopore
#6
Libo Zhu, Dejian Gu, Quanjun Liu
There are many techniques for the detection of molecules. But detection of molecules through solid-state nanopore in a solution is one of the promising, high-throughput, and low-cost technology used these days. In the present investigation, a solid-state nanopore platform was fabricated for the detection of hydrogen peroxide (H2O2), which is not only a label free product but also a significant participant in the redox reaction. We have successfully fabricated silicon nitride (Si3N4) nanopores with diameters of ~50 nm by using a focused Ga ion beam, the inner surface of the nanopore has been modified with horseradish peroxidase (HRP) by employing carbodiimide coupling chemistry...
December 2017: Nanoscale Research Letters
https://www.readbyqxmd.com/read/28636451/assessing-hydrogen-peroxide-vapor-exposure-from-hospital-sterilizers
#7
Robert Cornelia, P Richard Warburton
This study examined the hydrogen peroxide concentrations measured near four models of hydrogen peroxide sterilizers, from two manufacturers, monitored in seven hospitals across the US and Canada over several years. The results showed that the majority of sterilizers do not emit hazardous levels of hydrogen peroxide and none of them exceeded the OSHA PEL of 1 ppm (8 hour time weighted average (TWA)), however several of the sterilizers exceeded 3 ppm, the short term exposure limit in a couple of states, Washington and Hawaii...
June 21, 2017: Journal of Occupational and Environmental Hygiene
https://www.readbyqxmd.com/read/28634849/characterization-of-new-recombinant-3-ketosteroid-%C3%AE-1-dehydrogenases-for-the-biotransformation-of-steroids
#8
Xiaojun Wang, Jinhui Feng, Dalong Zhang, Qiaqing Wu, Dunming Zhu, Yanhe Ma
3-Ketosteroid-Δ(1)-dehydrogenases (KstDs [EC 1.3.99.4]) catalyze the Δ(1)-dehydrogenation of steroids and are a class of important enzymes for steroid biotransformations. In this study, we cloned 12 putative KstD-encoding (kstd) genes from both fungal and Gram-positive microorganisms and attempted to overproduce the recombinant proteins in E. coli BL21(DE3). Five successful recombinant enzymes catalyzed the Δ(1)-desaturation of a variety of steroidal compounds such as 4-androstene-3,17-dione (AD), 9α-hydroxy-4-androstene-3,17-dione (9-OH-AD), hydrocortisone, cortisone, and cortexolone...
June 20, 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28634477/metabolomic-analysis-and-mode-of-action-of-metabolites-of-tea-tree-oil-involved-in-the-suppression-of-botrytis-cinerea
#9
Jiayu Xu, Xingfeng Shao, Yonghua Li, Yingying Wei, Feng Xu, Hongfei Wang
Tea tree oil (TTO), a volatile essential oil, has been widely used as an antimicrobial agent. However, the mechanism underlying TTO antifungal activity is not fully understood. In this study, a comprehensive metabolomics survey was undertaken to identify changes in metabolite production in Botrytis cinerea cells treated with TTO. Significant differences in 91 metabolites were observed, including 8 upregulated and 83 downregulated metabolites in TTO-treated cells. The results indicate that TTO inhibits primary metabolic pathways through the suppression of the tricarboxylic acid (TCA) cycle and fatty acid metabolism...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28633841/novel-approaches-based-on-ultrasound-for-treatment-of-wastewater-containing-potassium-ferrocyanide
#10
Rajashree H Jawale, Akash Tandale, Parag R Gogate
Industrial wastewaters containing biorefractory compounds like cyanide offer significant environmental problems attributed to the fact that the conventional methods have limited effectiveness and hence developing efficient treatment approaches is an important requirement. The present work investigates the use of novel treatment approach of ultrasound (US) combined with advanced oxidation techniques for the degradation of potassium ferrocyanide (KFC) for the first time. An ultrasonic bath equipped with longitudinal horn (1kW rated power and 25kHz frequency) has been used...
September 2017: Ultrasonics Sonochemistry
https://www.readbyqxmd.com/read/28633507/digenic-duox1-and-duox2-mutations-in-cases-with-congenital-hypothyroidism
#11
Zehra Aycan, Hakan Cangul, Marina Muzza, Veysel N Bas, Laura Fugazzola, V Krishna Chatterjee, Luca Persani, Nadia Schoenmakers
Context: The DUOX2 enzyme generates hydrogen peroxide (H2O2), a crucial electron acceptor for the TPO-catalyzed iodination and coupling reactions mediating thyroid hormone biosynthesis. DUOX2 mutations result in dyshormonogenetic Congenital Hypothyroidism (CH) which may be phenotypically heterogeneous, leading to the hypothesis that CH severity may be influenced by environmental factors (eg dietary iodine) and oligogenic modifiers (eg variants in the homologous NADPH-oxidase DUOX1). However, loss of function mutations in DUOX1 have not hitherto been described and its role in thyroid biology remains undefined...
June 16, 2017: Journal of Clinical Endocrinology and Metabolism
https://www.readbyqxmd.com/read/28633086/beneficial-effects-of-melatonin-in-overcoming-drought-stress-in-wheat-seedlings
#12
Guibin Cui, Xiaoxiao Zhao, Shudong Liu, Fengli Sun, Chao Zhang, Yajun Xi
Melatonin plays an important role in abiotic stress in plant, but its role in wheat drought tolerance is less known. To verify its role, wheat seedlings (Triticum aestivum L. 'Yan 995') at 60% and 40% of field capacity were treated with 500 μM melatonin in this study. Melatonin treatment significantly enhanced the drought tolerance of wheat seedlings, as demonstrated by decreased membrane damage, more intact grana lamella of chloroplast, higher photosynthetic rate, and maximum efficiency of photosystem II, as well as higher cell turgor and water holding capacity in melatonin-treated seedlings...
June 15, 2017: Plant Physiology and Biochemistry: PPB
https://www.readbyqxmd.com/read/28631857/pd-catalyzed-hydroxylation-of-aryl-boronic-acids-using-in-situ-generated-hydrogen-peroxide
#13
Hong Yi, Aiwen Lei
In this work, we describe a benign and efficient palladium catalyzed in-situ generated hydrogen peroxide from carbon monoxide, water and oxygen under mild conditions. This novel methodology combines catalytic hydrogen peroxides production with aerobic oxidation process in a sole reaction system. This system shows good functional group tolerance and provides a benign and efficient access to a variety of functionalized phenols. Furthermore, the in-situ generated hydroperoxide can be well used for triphenylphosphine oxidation,in which the TON is up to 194...
June 20, 2017: Chemistry: a European Journal
https://www.readbyqxmd.com/read/28630667/evaluation-of-radical-scavenging-system-in-amoeba-chaos-carolinense-during-nutrient-deprivation
#14
Yuru Deng, Edlyn Li-Hui Lee, Ketpin Chong, Zakaria A Almsherqi
The frequent appearance of non-lamellar membrane arrangements such as cubic membranes (CMs) in cells under stressed or pathological conditions points to an intrinsic cellular response mechanism. CM represents highly curved, three-dimensional nano-periodic structures that correspond to mathematically well-defined triply periodic minimal surfaces. Specifically, cellular membrane may transform into CM organization in response to pathological, inflammatory and oxidative stress conditions. CM organization, thus, may provide an advantage to cope with various types of stress...
August 6, 2017: Interface Focus
https://www.readbyqxmd.com/read/28629707/theoretical-investigation-of-the-selective-dehydration-and-dehydrogenation-of-ethanol-catalyzed-by-small-molecules
#15
Yanqun Wang, Yizhen Tang, Youxiang Shao
Catalytic dehydration and dehydrogenation reactions of ethanol have been investigated systematically using the ab initio quantum chemistry methods The catalysts include water, hydrogen peroxide, formic acid, phosphoric acid, hydrogen fluoride, ammonia, and ethanol itself. Moreover, a few clusters of water and ethanol were considered to simulate the catalytic mechanisms in supercritical water and supercritical ethanol. The barriers for both dehydration and dehydrogenation can be reduced significantly in the presence of the catalysts...
May 17, 2017: Journal of Molecular Graphics & Modelling
https://www.readbyqxmd.com/read/28629110/recycling-the-liquid-fraction-of-alkaline-hydrogen-peroxide-in-the-pretreatment-of-corn-stover
#16
Bárbara Ribeiro Alves Alencar, Alexandre Libanio Silva Reis, Raquel de Fatima Rodrigues de Souza, Marcos Antônio Morais, Rômulo Simões Cezar Menezes, Emmanuel Damilano Dutra
The aim of this study was to evaluate the influence of recycling the liquid fraction of pretreatment with alkaline hydrogen peroxide (AHP) on the hydrolysis of corn stover. Corn stover was pretreated in the traditional condition with 7.5% v/v H2O2. After pretreatment, the solids were separated from the liquid fraction and five successive reuse cycles of the liquid fraction were tested. The solid fraction from pretreatment in each recycle was submitted to enzymatic hydrolysis. The number of recycles had a linear negative effect (R(2)=0...
June 8, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28628881/photodegradation-and-ecotoxicology-of-acyclovir-in-water-under-uv254-and-uv254-h2o2-processes
#17
Danilo Russo, Antonietta Siciliano, Marco Guida, Emilia Galdiero, Angela Amoresano, Roberto Andreozzi, Nuno M Reis, Gianluca Li Puma, Raffaele Marotta
The photochemical and ecotoxicological fate of acyclovir (ACY) through UV254 direct photolysis and in the presence of hydroxyl radicals (UV254/H2O2 process) were investigated in a microcapillary film (MCF) array photoreactor, which provided ultrarapid and accurate photochemical reaction kinetics. The UVC phototransformation of ACY was found to be unaffected by pH in the range from 4.5 to 8.0 and resembled an apparent autocatalytic reaction. The proposed mechanism included the formation of a photochemical intermediate (ϕACY = (1...
June 7, 2017: Water Research
https://www.readbyqxmd.com/read/28628200/local-generation-and-imaging-of-hydrogen-peroxide-in-living-cells
#18
Yulia A Bogdanova, Carsten Schultz, Vsevolod V Belousov
Described here is a localized H2 O2 generation-detection system consisting of a yeast D-amino acid oxidase (DAAO) and two spectrally distinct variants of biosensor, HyPer2 and HyPerRed based on circularly permutated yellow and red fluorescent proteins, respectively, which enables spatiotemporal production and examination of the intracellular H2 O2 dynamics. The protocol describes using this system in a simple cell culture model. We provide detailed instructions on imaging of H2 O2 generated by the activated DAAO...
June 19, 2017: Current Protocols in Chemical Biology
https://www.readbyqxmd.com/read/28627877/detection-and-quantification-of-hydrogen-peroxide-in-aqueous-solutions-using-chemical-exchange-saturation-transfer-cest
#19
David Ryoo, Xiang Xu, Yuguo Li, Joel Tang, Jia Zhang, Peter C M van Zijl, Guanshu Liu
The development of new analytical methods to accurately quantify hydrogen peroxide is of great interest. In the current study, we developed a new magnetic resonance (MR) method for noninvasively quantifying hydrogen peroxide (H2O2) in aqueous solutions based on chemical exchange saturation transfer (CEST), an emerging MRI contrast mechanism. Our method can detect H2O2 by its specific CEST signal at ~6.2 ppm downfield from water resonance, with more than 1000 times signal amplification compared to the direct NMR detection...
June 19, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28627761/biological-effects-of-hydrogen-peroxide-administered-intratumorally-with-or-without-irradiation-in-murine-tumors
#20
Taiki Takaoka, Yuta Shibamoto, Masayuki Matsuo, Chikao Sugie, Taro Murai, Yasutaka Ogawa, Akifumi Miyakawa, Yoshihiko Manabe, Takuhito Kondo, Koichiro Nakajima, Dai Okazaki, Takahiro Tsuchiya
Despite insufficient laboratory data, radiotherapy after intratumoral injection of hydrogen peroxide (H2 O2 ) is increasingly being used clinically for radioresistant tumors. Especially, this treatment might become an alternative definitive treatment for early and advanced breast cancer in patients who refuse any type of surgery. The purpose of this study was to investigate the biological effects and appropriate combination methods of irradiation and H2 O2 in vivo. SCCVII tumor cells transplanted into the legs of C3H/HeN mice were used...
June 19, 2017: Cancer Science
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