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https://www.readbyqxmd.com/read/29778001/patchy-gold-coated-fe-3-o-4-nanospheres-with-enhanced-catalytic-activity-applied-for-paper-based-bipolar-electrode-electrochemiluminescence-aptasensors
#1
Xin Zhang, Ning Bao, Xiliang Luo, Shou-Nian Ding
In this work, novel multifunctional patchy gold coated Fe3 O4 hybrid nanoparticles (PG-Fe3 O4 NPs) have been successfully synthesized in aqueous medium via a facile adsorption-reduction method. A rational formation mechanism has been proposed by monitoring the morphological evolution. The PG-Fe3 O4 NPs retained the good magnetic property and exhibited excellent catalytical effeciency towards the electrochemical reduction of hydrogen peroxide. Chronoamperometric and amperometric experiments indicated a relatively high catalytic rate constant of 3...
May 10, 2018: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/29777313/gold-nanocluster-based-ratiometric-fluorescent-probes-for-hydrogen-peroxide-and-enzymatic-sensing-of-uric-acid
#2
Dongqin Yang, Minchuan Luo, Junwei Di, Yifeng Tu, Jilin Yan
A method is described for ratiometric fluorometric assays of H2 O2  by using two probes that have distinct response profiles. Under the catalytic action of ferrous ion, the 615 nm emission of protein-stabilized gold nanoclusters (under 365 nm photoexcitation) is quenched by H2 O2 , while an increased signal is generated with a peak at 450 nm by oxidizing coumarin with the H2 O2 /Fe(II) system to form a blue emitting fluorophore. These decrease/increase responses give a ratiometric signal. The ratio of the fluorescences at the two peaks are linearly related to the concentration of H2 O2 in the range from 0...
May 18, 2018: Mikrochimica Acta
https://www.readbyqxmd.com/read/29776543/a-near-infrared-ratiometric-turn-on-fluorescent-probe-for-in-vivo-imaging-of-hydrogen-peroxide-in-a-murine-model-of-acute-inflammation
#3
Jingli Hou, Meng Qian, Huanhuan Zhao, Yingchun Li, Yongfang Liao, Guifang Han, Zhelong Xu, Feng Wang, Yuguang Song, Yangping Liu
Much attention has been paid to develop optical probes for noninvasive, quantitative, in vivo monitoring of hydrogen peroxide (H2 O2 ) due to its important roles in the initiation and development of numerous diseases. Motivated to meet this need, we herein report the synthesis of a near-infrared (NIR) fluorescent probe (AB1) for H2 O2 by modulating intramolecular charge transfer (ICT) process of the dye 9H-1,3-Dichloro-7-hydroxy-9,9-dimethylacridine-2-one (DDAO). The probe AB1 exhibits both a large NIR fluorescence turn-on and a ratiometric response to H2 O2 with high sensitivity and specificity...
September 18, 2018: Analytica Chimica Acta
https://www.readbyqxmd.com/read/29775864/exogenous-hydrogen-peroxide-inhibits-primary-root-gravitropism-by-regulating-auxin-distribution-during-arabidopsis-seed-germination
#4
Lina Zhou, Hongzhou Hou, Tao Yang, Yuke Lian, Yan Sun, Zhiyuan Bian, Chongying Wang
Hydrogen peroxide (H2 O2 ) is the key factor in many physiological and metabolic processes in plants. During seed germination, exogenous H2 O2 application influences gravitropism and induces curvature of the primary root in grass pea and pea seedlings. However, it remains unclear whether and how this happens in the model plant Arabidopsis thaliana. In the present study, the effect of exogenous H2 O2 on the gravitropic response of primary roots during Arabidopsis seed germination was studied using histology and molecular biology approaches...
May 15, 2018: Plant Physiology and Biochemistry: PPB
https://www.readbyqxmd.com/read/29775686/the-comparative-efficacy-of-antiseptics-against-candida-auris-biofilms
#5
Ryan Kean, Emily McKloud, Eleanor M Townsend, Leighann Sherry, Christopher Delaney, Brian L Jones, Craig Williams, Gordon Ramage
Candida auris has emerged as a significant clinical entity due to its ability to cause outbreaks within the healthcare setting. A key feature of its nosocomial lifestyle is its ability to transfer between patients, yet little is known about the mechanisms behind this. A panel of C. auris clinical isolates was screened for their planktonic and sessile susceptibilities to skin disinfection challenge using povidone iodine, chlorhexidine and hydrogen peroxide. C. auris biofilms displayed increased tolerance to these strategies compared to planktonic cells...
May 15, 2018: International Journal of Antimicrobial Agents
https://www.readbyqxmd.com/read/29775644/redox-signal-mediated-trpm2-promotes-ang-ii-induced-adipocyte-insulin-resistance-via-ca-2-dependent-camkii-jnk-cascade
#6
Min Gao, Yu Du, Jing-Wen Xie, Jing Xue, Yi-Ting Wang, Li Qin, Ming-Ming Ma, Yong-Bo Tang, Xiao-Yan Li
BACKGROUND AND OBJECTIVE: Redox-sensitive transient receptor potential melastatin 2 (TRPM2) is a Ca2+ -permeable, nonselective cation channel which plays a crucial role in various physiological processes. However, little is known whether TRPM2 is involved in adipocyte dysfunction during hypertension. In the present study, we determined the role of TRPM2 in angiotensin II (Ang II)-induced insulin resistance in adipocytes and the underlying mechanisms. METHODS: Ang II-induced adipocyte insulin resistant model was conducted...
May 15, 2018: Metabolism: Clinical and Experimental
https://www.readbyqxmd.com/read/29775394/degradation-of-ticarcillin-by-subcritial-water-oxidation-method-application-of-response-surface-methodology-and-artificial-neural-network-modeling
#7
Erdal Yabalak
This study was performed to investigate the mineralization of ticarcillin in the artificially prepared aqueous solution presenting ticarcillin contaminated waters, which constitute a serious problem for human health. 81.99% of total organic carbon removal, 79.65% of chemical oxygen demand removal, and 94.35% of ticarcillin removal were achieved by using eco-friendly, time-saving, powerful and easy-applying, subcritical water oxidation method in the presence of a safe-to-use oxidizing agent, hydrogen peroxide...
May 18, 2018: Journal of Environmental Science and Health. Part A, Toxic/hazardous Substances & Environmental Engineering
https://www.readbyqxmd.com/read/29774550/efficacy-of-vaporized-hydrogen-peroxide-for-repeated-sterilization-of-a-single-use-single-incision-laparoscopic-surgery-port
#8
Valery F Scharf, Brian Dent, Megan E Jacob, Brandon Moore
OBJECTIVE: To determine the ability of vaporized hydrogen peroxide (VHP) to sterilize a single-use single-incision laparoscopic surgery port and its associated components after repeated simulated uses. STUDY DESIGN: Prospective in vitro experimental study. SAMPLE POPULATION: Six single-use single-incision laparoscopic surgery ports with associated cannulas and insufflation tubing. METHODS: Ports, cannulas, and tubing were subjected to 10 cycles of simulated use, bacterial inoculation with Staphylococcus pseudintermedius and Escherichia coli, decontamination and sterilization, and testing via culture based on their treatment group designation...
May 17, 2018: Veterinary Surgery: VS
https://www.readbyqxmd.com/read/29772776/pyrrole-alkaloids-from-the-edible-mushroom-phlebopus-portentosus-with-their-bioactive-activities
#9
Zhaocui Sun, Meigeng Hu, Zhonghao Sun, Nailiang Zhu, Junshan Yang, Guoxu Ma, Xudong Xu
Seven pyrrole alkaloids, three of which are novel (phlebopines A⁻C ( 1 ⁻ 3 )), were isolated from the fruiting bodies of the edible mushroom Phlebopus portentosus . Their structures were determined on the basis of spectroscopic data. All the isolated compounds were tested for their neuroprotective properties and acetylcholine esterase (AChE) inhibition activities. Compound 7 displayed remarkable neuroprotective effects against hydrogen peroxide (H₂O₂)-induced neuronal-cell damage in human neuroblastoma SH-SY5Y cells...
May 17, 2018: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/29770941/degradation-of-ampicillin-antibiotic-by-electrochemical-processes-evaluation-of-antimicrobial-activity-of-treated-water
#10
Jorge Vidal, Cesar Huiliñir, Rocío Santander, Javier Silva-Agredo, Ricardo A Torres-Palma, Ricardo Salazar
Ampicillin (AMP) is an antibiotic widely used in hospitals and veterinary clinics around the world for treating infections caused by bacteria. Therefore, it is common to find traces of this antibiotic in wastewater from these entities. In this work, we studied the mineralization of this antibiotic in solution as well as the elimination of its antimicrobial activity by comparing different electrochemical advanced oxidation processes (EAOPs), namely electro-oxidation with hydrogen peroxide (EO-H2 O2 ), electro-Fenton (EF), and photo electro-Fenton (PEF)...
May 17, 2018: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/29770908/level-of-hydrogen-peroxide-affects-expression-and-sub-cellular-localization-of-pax6
#11
Sachin Shukla, Rajnikant Mishra
The Pax6 is a multifunctional pairedbox and homeobox containing transcription factor which is involved in several functions of brain, eyes, and pancreas. It regulates expression of genes involved in cell proliferation, differentiation, inflammation, oxidative stress management, and neuropathy. Dynamic changes in the sub-cellular localization of Pax6 are proposed to regulate its activity, however, the underlying mechanism remains poorly understood. The oxidative stress mediated changes were studied in sub-cellular localization of Pax6 in cultured cells derived from the eye (cornea) and pancreas...
May 16, 2018: Molecular Biology Reports
https://www.readbyqxmd.com/read/29770867/dietary-supplementation-with-an-amino-acid-blend-enhances-intestinal-function-in-piglets
#12
Dan Yi, Baocheng Li, Yongqing Hou, Lei Wang, Di Zhao, Hongbo Chen, Tao Wu, Ying Zhou, Binying Ding, Guoyao Wu
The traditionally classified nutritionally non-essential amino acids are now known to be insufficiently synthesized for maximal growth and optimal health in piglets. This study determined the effects of dietary supplementation with an amino acid blend (AAB; glutamate:glutamine:glycine:arginine:N-acetylcysteine = 5:2:2:1:0.5) on piglet growth performance and intestinal functions. Sixteen piglets (24-day-old) were randomly assigned to a corn and soybean meal-based diet supplemented with 0.99% alanine (isonitrogenous control) or 1% AAB...
May 16, 2018: Amino Acids
https://www.readbyqxmd.com/read/29770859/ionic-liquid-coated-iron-nanoparticles-are-promising-peroxidase-mimics-for-optical-determination-of-h-2-o-2
#13
Faiza Zarif, Sajid Rauf, Muhammad Zahid Qureshi, Noor Samad Shah, Akhtar Hayat, Nawshad Muhammad, Abdur Rahim, Mian Hasnain Nawaz, Muhammad Nasir
Ionic liquid coated nanoparticles (IL-NPs) consisting of zero-valent iron are shown to display intrinsic peroxidase-like activity with enhanced potential to catalyze the oxidation of the chromogenic substrate 3,3',5,5'-tetramethylbenzidine (TMB) in the presence of hydrogen peroxide. This results in the formation of a blue green colored product that can be detected with bare eyes and quantified by photometry at 652 nm. The IL-NPs were further doped with bismuth to enhance its catalytic properties. The Bi-doped IL-NPs were characterized by FTIR, X-ray diffraction and scanning electron microscopy...
May 16, 2018: Mikrochimica Acta
https://www.readbyqxmd.com/read/29770164/aquaporin-membrane-channels-in-oxidative-stress-cell-signaling-and-aging-recent-advances-and-research-trends
#14
REVIEW
Grazia Tamma, Giovanna Valenti, Elena Grossini, Sandra Donnini, Angela Marino, Raul A Marinelli, Giuseppe Calamita
Reactive oxygen species (ROS) are produced as a result of aerobic metabolism and as by-products through numerous physiological and biochemical processes. While ROS-dependent modifications are fundamental in transducing intracellular signals controlling pleiotropic functions, imbalanced ROS can cause oxidative damage, eventually leading to many chronic diseases. Moreover, increased ROS and reduced nitric oxide (NO) bioavailability are main key factors in dysfunctions underlying aging, frailty, hypertension, and atherosclerosis...
2018: Oxidative Medicine and Cellular Longevity
https://www.readbyqxmd.com/read/29767342/characterization-of-xanthine-dehydrogenase-and-aldehyde-oxidase-of-marsupenaeus-japonicus-and-their-response-to-microbial-pathogen
#15
Yo Okamura, Mari Inada, Gehad Elsaid Elshopakey, Toshiaki Itami
Reactive oxygen species (ROS) play key roles in many physiological processes. In particular, the sterilization mechanism of bacteria using ROS in macrophages is a very important function for biological defense. Xanthine dehydrogenase (XDH) and aldehyde oxidase (AOX), members of the molybdo-flavoenzyme subfamily, are known to generate ROS. Although these enzymes occur in many vertebrates, some insects, and plants, little research has been conducted on XDHs and AOXs in crustaceans. Here, we cloned the entire cDNA sequences of XDH (MjXDH: 4328 bp) and AOX (MjAOX: 4425 bp) from Marsupenaeus japonicus (kuruma shrimp) using reverse transcriptase-polymerase chain reaction (RT-PCR) and random amplification of cDNA ends (RACE)...
May 16, 2018: Molecular Biology Reports
https://www.readbyqxmd.com/read/29767257/bmp%C3%A2-6-protects-retinal-pigment-epithelial-cells-from-oxidative-stress%C3%A2-induced-injury-by-inhibiting-the-mapk-signaling-pathways
#16
Li Chen, Ming Liu, Yan Luan, Yingfei Liu, Zhichao Zhang, Bo Ma, Xuan Liu, Yong Liu
Worldwide, neovascular age‑related macular degeneration (nAMD) is one of the most common causes of blindness in the elderly. In particular, degeneration of retinal pigment epithelial (RPE) cells represents the main pathological process in the development of nAMD, and oxidative stress serves a major role. The present study aimed to investigate the association between bone morphogenetic protein 6 (BMP‑6) and nAMD. BMP‑6 concentration was significantly reduced in patients with wet nAMD compared with in the control group...
May 14, 2018: International Journal of Molecular Medicine
https://www.readbyqxmd.com/read/29767231/bidirectional-modulation-of-insulin-action-by-reactive-oxygen-species-in-3t3%C3%A2-l1-adipocytes
#17
Mingfeng Ma, Yingyao Quan, Yong Li, Xu He, Jing Xiao, Meixiao Zhan, Wei Zhao, Yongjie Xin, Ligong Lu, Liangping Luo
Reactive oxygen species (ROS) serve an important role in glucose‑lipid metabolic regulation. In the present study, the results demonstrated that there was bidirectional regulation of insulin action in 3T3‑L1 adipocytes treated with ROS. Transient and acute ROS exposure improved insulin‑induced metabolic effects in 3T3‑L1 adipocytes. Hydrogen peroxide (H2O2), as a stable and diffusible ROS, diffused into adipocytes and altered intracellular redox homeostasis, resulting in oxidation and inactivation of phosphatase and tensin homologue deleted on chromosome 10 (PTEN)...
May 14, 2018: Molecular Medicine Reports
https://www.readbyqxmd.com/read/29767192/ultra-low-voltage-triggered-release-of-an-anti-cancer-drug-from-polypyrrole-nanoparticles
#18
Devleena Samanta, Niloufar Hosseini-Nassab, Aidan D McCarty, Richard N Zare
We have synthesized polypyrrole nanoparticles using three different oxidizing agents (hydrogen peroxide, chloroauric acid and ferric chloride) and shown that films assembled from these nanoparticles have significantly different drug release profiles. When ferric chloride is used as the oxidizing agent, it is possible to release drugs at voltages as low as -0.05 V, almost an order of magnitude lower than typically used voltages. These ultra-low voltage responsive nanoparticles widen the window of operation of conducting polymers and enable delivery of redox active drugs...
May 16, 2018: Nanoscale
https://www.readbyqxmd.com/read/29766712/highly-sensitive-membrane-based-pressure-sensors-meps-for-real-time-monitoring-of-catalytic-reactions
#19
Alessandra Zizzari, Monica Bianco, Loretta L Del Mercato, Antonio Sorarù, Mauro Carraro, Paolo Pellegrino, Elisabetta Perrone, Anna Grazia Monteduro, Marcella Bonchio, Rosaria Rinaldi, Ilenia Viola, Valentina Arima
Functional, flexible and integrated lab-on-chips, based on elastic membranes, are capable of fine response to external stimuli, so to pave the way for many applications as multiplexed sensors for a wide range of chemical, physical and biomedical processes. Here, we report on the use of elastic thin membranes (TMs), integrated with a reaction chamber, to fabricate a Membrane-based Pressure Sensor (MePS) for reaction monitoring. In particular, the TM becomes the key-element in the design of a highly sensitive MePS capable to monitor gaseous species production in dynamic and temporally fast processes with high resolution and reproducibility...
May 16, 2018: Analytical Chemistry
https://www.readbyqxmd.com/read/29766639/oxidation-resistance-1-is-a-novel-senolytic-target
#20
Xin Zhang, Suping Zhang, Xingui Liu, Yingying Wang, Jianhui Chang, Xuan Zhang, Samuel G Mackintosh, Alan J Tackett, Yonghan He, Dongwen Lv, Remi-Martin Laberge, Judith Campisi, Jianrong Wang, Guangrong Zheng, Daohong Zhou
The selective depletion of senescent cells (SCs) by small molecules, termed senolytic agents, is a promising therapeutic approach for treating age-related diseases and chemotherapy- and radiotherapy-induced side effects. Piperlongumine (PL) was recently identified as a novel senolytic agent. However, its mechanism of action and molecular targets in SCs was unknown and thus was investigated. Specifically, we used a PL-based chemical probe to pull-down PL-binding proteins from live cells and then mass spectrometry-based proteomic analysis to identify potential molecular targets of PL in SCs...
May 15, 2018: Aging Cell
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