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https://www.readbyqxmd.com/read/29090830/a-novel-biosensor-with-high-signal-to-noise-ratio-for-real-time-measurement-of-dopamine-levels-in-vivo
#1
Akimasa Ishida, Atsushi Imamura, Yoshitomo Ueda, Takeshi Shimizu, Ryosuke Marumoto, Cha-Gyun Jung, Hideki Hida
Fast-scan cyclic voltammetry (FSCV) is an established method for measuring dopamine (DA) levels in the brain in real time. However, it is difficult to discriminate DA from other monoamines such as serotonin (5-hydroxytryptamine, 5-HT) and norepinephrine (NE). We report a novel DA-specific biosensor consisting of a carbon-fiber electrode coated with an ion-exchange membrane, a layer containing monoamine oxidase B, and a cellulose membrane. We performed FSCV using the probe to monitor the amount of DA in vitro and in vivo...
November 1, 2017: Journal of Neuroscience Research
https://www.readbyqxmd.com/read/29063091/a-baseline-drift-detrending-technique-for-fast-scan-cyclic-voltammetry
#2
Mark DeWaele, Yoonbae Oh, Cheonho Park, Yu Min Kang, Hojin Shin, Charles D Blaha, Kevin E Bennet, In Young Kim, Kendall H Lee, Dong Pyo Jang
Fast scan cyclic voltammetry (FSCV) has been commonly used to measure extracellular neurotransmitter concentrations in the brain. Due to the unstable nature of the background currents inherent in FSCV measurements, analysis of FSCV data is limited to very short amounts of time using traditional background subtraction. In this paper, we propose the use of a zero-phase high pass filter (HPF) as the means to remove the background drift. Instead of the traditional method of low pass filtering across voltammograms to increase the signal to noise ratio, a HPF with a low cutoff frequency was applied to the temporal dataset at each voltage point to remove the background drift...
October 24, 2017: Analyst
https://www.readbyqxmd.com/read/28943284/evaluating-oral-flavorant-effects-on-nicotine-self-administration-behavior-and-phasic-dopamine-signaling
#3
Robert J Wickham, Eric J Nunes, Shannon Hughley, Phillip Silva, Sofia N Walton, Jinwoo Park, Nii A Addy
Understanding how tobacco product flavor additives, such as flavorants in electronic cigarettes, influence smoking behavior and addiction is critical for informing public health policy decisions regarding tobacco product regulation. Here, we developed a combined intraoral (i.o.) and intravenous (i.v.) self-administration paradigm in rats to determine how flavorants influence self-administration behavior. By combining i.o. flavorant delivery with fast scan cyclic voltammetry (FSCV) or i.v. nicotine self-administration in adult, male rats, we examined whether flavors alter phasic dopamine (DA) signaling and nicotine self-administration...
September 21, 2017: Neuropharmacology
https://www.readbyqxmd.com/read/28918253/deep-brain-stimulation-of-the-medial-forebrain-bundle-elevates-striatal-dopamine-concentration-without-affecting-spontaneous-or-reward-induced-phasic-release
#4
Marianne Klanker, Matthijs Feenstra, Ingo Willuhn, Damiaan Denys
Deep brain stimulation (DBS) of the medial forebrain bundle (MFB) induces rapid improvement of depressive symptoms in patients suffering from treatment-refractory major depressive disorder (MDD). It has been hypothesized that activation of the dopamine (DA) system contributes to this effect. To investigate whether DBS in the MFB affects DA release in the striatum, we combined DBS with fast-scan cyclic voltammetry (FSCV) in freely moving rats. Animals were implanted with a stimulating electrode at the border of the MFB and the ventral tegmental area, and a FSCV microelectrode in the ventromedial striatum to monitor extracellular DA during the acute onset of DBS and subsequent continued stimulation...
November 19, 2017: Neuroscience
https://www.readbyqxmd.com/read/28840722/multivariate-curve-resolution-for-signal-isolation-from-fast-scan-cyclic-voltammetric-data
#5
Justin A Johnson, Josh H Gray, Nathan T Rodeberg, R Mark Wightman
The use of multivariate analysis techniques, such as principal component analysis-inverse least-squares (PCA-ILS), has become standard for signal isolation from in vivo fast-scan cyclic voltammetric (FSCV) data due to its superior noise removal and interferent-detection capabilities. However, the requirement of collecting separate training data for PCA-ILS model construction increases experimental complexity and, as such, has been the source of recent controversy. Here, we explore an alternative method, multivariate curve resolution-alternating least-squares (MCR-ALS), to circumvent this issue while retaining the advantages of multivariate analysis...
September 13, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28795565/in-vivo-ambient-serotonin-measurements-at-carbon-fiber-microelectrodes
#6
Aya Abdalla, Christopher W Atcherley, Pavithra Pathirathna, Srimal Samaranayake, Beidi Qiang, Edsel Peña, Stephen L Morgan, Michael L Heien, Parastoo Hashemi
The mechanisms that control extracellular serotonin levels in vivo are not well-defined. This shortcoming makes it very challenging to diagnose and treat the many psychiatric disorders in which serotonin is implicated. Fast-scan cyclic voltammetry (FSCV) can measure rapid serotonin release and reuptake events but cannot report critically important ambient serotonin levels. In this Article, we use fast-scan controlled adsorption voltammetry (FSCAV), to measure serotonin's steady-state, extracellular chemistry...
September 7, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28758383/single-organic-droplet-collision-voltammogram-via-electron-transfer-coupled-ion-transfer
#7
Cheng Liu, Pekka Peljo, Xinjian Huang, Wenxue Cheng, Lishi Wang, Haiqiang Deng
Single-emulsion toluene oil droplets (femtoliter) containing a hydrophobic redox probe that are dispersed in water stochastically collide with an ultramicroelectrode (UME). The fast-scan cyclic voltammetry (FSCV) or Fourier-transformed sinusoidal voltammetry (FTSV) is applied: the UME was scanned with a fast, repetitive triangular, or sinusoidal potential, and its current in time/frequency domains were monitored. The electron transfer at the UME/oil interface is coupled with ion transfer at the oil/water interface...
September 5, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28667509/hypocretin-receptor-1-blockade-produces-bimodal-modulation-of-cocaine-associated-mesolimbic-dopamine-signaling
#8
K A Levy, Z D Brodnik, J K Shaw, D A Perrey, Y Zhang, R A España
RATIONALE: Cocaine addiction is a chronic psychiatric disorder characterized by pathological motivation to obtain cocaine and behavioral and neurochemical hypersensitivity to cocaine-associated cues. These features of cocaine addiction are thought to be driven by aberrant phasic dopamine signaling. We previously demonstrated that blockade of the hypocretin receptor 1 (HCRTr1) attenuates cocaine self-administration and reduces cocaine-induced enhancement of dopamine signaling. Despite this evidence, the effects of HCRTr1 blockade on endogenous phasic dopamine release are unknown...
June 30, 2017: Psychopharmacology
https://www.readbyqxmd.com/read/28619545/blast-related-disinhibition-and-risk-seeking-in-mice-and-combat-veterans-potential-role-for-dysfunctional-phasic-dopamine-release
#9
A G Schindler, J S Meabon, K F Pagulayan, R C Hendrickson, K D Meeker, M Cline, G Li, C Sikkema, C W Wilkinson, D P Perl, M R Raskind, E R Peskind, J J Clark, D G Cook
Mild traumatic brain injury (mTBI) caused by exposure to high explosives has been called the "signature injury" of the wars in Iraq and Afghanistan. There is a wide array of chronic neurological and behavioral symptoms associated with blast-induced mTBI. However, the underlying mechanisms are not well understood. Here we used a battlefield-relevant mouse model of blast-induced mTBI and in vivo fast-scan cyclic voltammetry (FSCV) to investigate whether the mesolimbic dopamine system contributes to the mechanisms underlying blast-induced behavioral dysfunction...
October 2017: Neurobiology of Disease
https://www.readbyqxmd.com/read/28617576/ex-vivo-measurement-of-electrically-evoked-dopamine-release-in-zebrafish-whole-brain
#10
Mimi Shin, Thomas M Field, Chase S Stucky, Mia N Furgurson, Michael A Johnson
Zebrafish (Danio rerio) have recently emerged as useful model organism for the study of neuronal function. Here, fast-scan cyclic voltammetry (FSCV) at carbon-fiber microelectrodes was used to measure locally evoked dopamine release and uptake in zebrafish whole brain preparations and results were compared with those obtained from brain slices. Evoked dopamine release ([DA]max) was similar in whole brain and sagittal brain slice preparations (0.49 ± 0.13 μM in whole brain and 0.59 ± 0.28 μM in brain slices)...
September 20, 2017: ACS Chemical Neuroscience
https://www.readbyqxmd.com/read/28605373/modeling-fast-scan-cyclic-voltammetry-data-from-electrically-stimulated-dopamine-neurotransmission-data-using-qnsim1-0
#11
Rashed Harun, Christine M Grassi, Miranda J Munoz, Amy K Wagner
Central dopaminergic (DAergic) pathways have an important role in a wide range of functions, such as attention, motivation, and movement. Dopamine (DA) is implicated in diseases and disorders including attention deficit hyperactivity disorder, Parkinson's disease, and traumatic brain injury. Thus, DA neurotransmission and the methods to study it are of intense scientific interest. In vivo fast-scan cyclic voltammetry (FSCV) is a method that allows for selectively monitoring DA concentration changes with fine temporal and spatial resolution...
June 5, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/28597913/inherent-vulnerabilities-in-monoaminergic-pathways-predict-the-emergence-of-depressive-impairments-in-an-animal-model-of-chronic-epilepsy
#12
Jesús-Servando Medel-Matus, Don Shin, Raman Sankar, Andrey Mazarati
The objective was to determine whether the depression comorbid with epilepsy could be predicted based on inherent premorbid patterns of monoaminergic transmission. In male Wistar rats, despair-like and anhedonia-like behaviors were examined using forced swimming and taste preference tests, respectively. Serotonergic raphe nucleus (RN)-prefrontal cortex (PFC) and dopaminergic ventral tegmental area (VTA)-nucleus accumbens (NAcc) pathways were interrogated by fast scan cyclic voltammetry (FSCV). The assays were performed before and 2 months after pilocarpine status epilepticus...
August 2017: Epilepsia
https://www.readbyqxmd.com/read/28488873/removal-of-differential-capacitive-interferences-in-fast-scan-cyclic-voltammetry
#13
Justin A Johnson, Caddy N Hobbs, R Mark Wightman
Due to its high spatiotemporal resolution, fast-scan cyclic voltammetry (FSCV) at carbon-fiber microelectrodes enables the localized in vivo monitoring of subsecond fluctuations in electroactive neurotransmitter concentrations. In practice, resolution of the analytical signal relies on digital background subtraction for removal of the large current due to charging of the electrical double layer as well as surface faradaic reactions. However, fluctuations in this background current often occur with changes in the electrode state or ionic environment, leading to nonspecific contributions to the FSCV data that confound data analysis...
June 6, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28315693/central-glp-1-receptor-activation-modulates-cocaine-evoked-phasic-dopamine-signaling-in-the-nucleus-accumbens-core
#14
Samantha M Fortin, Mitchell F Roitman
Drugs of abuse increase the frequency and magnitude of brief (1-3s), high concentration (phasic) dopamine release events in terminal regions. These are thought to be a critical part of drug reinforcement and ultimately the development of addiction. Recently, metabolic regulatory peptides, including the satiety signal glucagon-like peptide-1 (GLP-1), have been shown to modulate cocaine reward-driven behavior and sustained dopamine levels after cocaine administration. Here, we use fast-scan cyclic voltammetry (FSCV) to explore GLP-1 receptor (GLP-1R) modulation of dynamic dopamine release in the nucleus accumbens (NAc) during cocaine administration...
March 16, 2017: Physiology & Behavior
https://www.readbyqxmd.com/read/28225988/estimation-of-cardiovascular-risk-and-detection-of-subclinical-carotid-atheromatosis-in-patients-with-diabetes-without-a-history-of-cardiovascular-disease
#15
MULTICENTER STUDY
Walter Masson, Salvador De Francesca, Micaela Molinero, Daniel Siniawski, Andrés Mulassi, Frank Espinoza Morales, Melina Huerin, Martín Lobo, Graciela Molinero
Objectives: Cardiovascular risk estimated by several scores in patients with diabetes mellitus without a cardiovascular disease history and the association with carotid atherosclerotic plaque (CAP) were the aims of this study. Materials and methods: Cardiovascular risk was calculate using United Kingdom Prospective Diabetes Study (UKPDS) risk engine, Framingham risk score for cardiovascular (FSCV) and coronary disease (FSCD), and the new score (NS) proposed by the 2013 ACC/AHA Guideline on the Treatment of Blood Cholesterol...
March 2017: Archives of Endocrinology and Metabolism
https://www.readbyqxmd.com/read/28223145/rapid-induction-of-dopamine-sensitization-in-the-nucleus-accumbens-shell-induced-by-a-single-injection-of-cocaine
#16
Bryan F Singer, Myranda A Bryan, Pavlo Popov, Terry E Robinson, Brandon J Aragona
Repeated intermittent exposure to cocaine results in the neurochemical sensitization of dopamine (DA) transmission within the nucleus accumbens (NAc). Indeed, the excitability of DA neurons in the ventral tegmental area (VTA) is enhanced within hours of initial psychostimulant exposure. However, it is not known if this is accompanied by a comparably rapid change in the ability of cocaine to increase extracellular DA concentrations in the ventral striatum. To address this question we used fast-scan cyclic voltammetry (FSCV) in awake-behaving rats to measure DA responses in the NAc shell following an initial intravenous cocaine injection, and then again 2-h later...
May 1, 2017: Behavioural Brain Research
https://www.readbyqxmd.com/read/28196418/automated-algorithm-for-detection-of-transient-adenosine-release
#17
Ryan P Borman, Ying Wang, Michael D Nguyen, Mallikarjunarao Ganesana, Scott T Lee, B Jill Venton
Spontaneous adenosine release events have been discovered in the brain that last only a few seconds. The identification of these adenosine events from fast-scan cyclic voltammetry (FSCV) data is difficult due to the random nature of adenosine release. In this study, we develop an algorithm that automatically identifies and characterizes adenosine transient features, including event time, concentration, and duration. Automating the data analysis reduces analysis time from 10 to 18 h to about 40 min per experiment...
February 15, 2017: ACS Chemical Neuroscience
https://www.readbyqxmd.com/read/28186690/reduced-levels-of-cacna1c-attenuate-mesolimbic-dopamine-system-function
#18
C E Terrillion, D T Dao, R Cachope, M K Lobo, A C Puche, J F Cheer, T D Gould
Genetic variation in CACNA1C, which codes for the L-type calcium channel (LTCC) Cav 1.2, is associated with clinical diagnoses of bipolar disorder, depression and schizophrenia. Dysregulation of the mesolimbic-dopamine (ML-DA) system is linked to these syndromes and LTCCs are required for normal DAergic neurotransmission between the ventral tegmental area (VTA) and nucleus accumbens (NAc). It is unclear, however, how variations in CACNA1C genotype, and potential subsequent changes in expression levels in these regions, modify risk...
June 2017: Genes, Brain, and Behavior
https://www.readbyqxmd.com/read/28127962/hitchhiker-s-guide-to-voltammetry-acute-and-chronic-electrodes-for-in-vivo-fast-scan-cyclic-voltammetry
#19
REVIEW
Nathan T Rodeberg, Stefan G Sandberg, Justin A Johnson, Paul E M Phillips, R Mark Wightman
Fast-scan cyclic voltammetry (FSCV) has been used for over 20 years to study rapid neurotransmission in awake and behaving animals. These experiments were first carried out with carbon-fiber microelectrodes (CFMs) encased in borosilicate glass, which can be inserted into the brain through micromanipulators and guide cannulas. More recently, chronically implantable CFMs constructed with small diameter fused-silica have been introduced. These electrodes can be affixed in the brain with minimal tissue response, which permits longitudinal measurements of neurotransmission in single recording locations during behavior...
February 15, 2017: ACS Chemical Neuroscience
https://www.readbyqxmd.com/read/28044445/background-signal-as-an-in-situ-predictor-of-dopamine-oxidation-potential-improving-interpretation-of-fast-scan-cyclic-voltammetry-data
#20
Carl J Meunier, James G Roberts, Gregory S McCarty, Leslie A Sombers
Background-subtracted fast-scan cyclic voltammetry (FSCV) has emerged as a powerful analytical technique for monitoring subsecond molecular fluctuations in live brain tissue. Despite increasing utilization of FSCV, efforts to improve the accuracy of quantification have been limited due to the complexity of the technique and the dynamic recording environment. It is clear that variable electrode performance renders calibration necessary for accurate quantification; however, the nature of in vivo measurements can make conventional postcalibration difficult, or even impossible...
February 15, 2017: ACS Chemical Neuroscience
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