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Fast scan cyclic voltammetry

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https://www.readbyqxmd.com/read/29137797/fabrication-of-g-c3n4-nio-heterostructured-nanocomposite-modified-glassy-carbon-electrode-for-quercetin-biosensor
#1
S Selvarajan, A Suganthi, M Rajarajan
Herein, we report a one-pot synthesis of structurally uniform and electrochemically active graphitic carbon nitride/nickel oxide (g-C3N4/NiO) nanocomposite and an investigation on the electrocatalytic oxidation of quercetin (QR). The synthesized g-C3N4/NiO nanocomposite has uniform surface distribution, which was characterized with scanning electron microscopy (SEM). Moreover, the composition of synthesized g-C3N4/NiO nanocomposite was characterized by UV-vis-spectroscopy, X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR spectra), BET, SEM and HRTEM...
March 2018: Ultrasonics Sonochemistry
https://www.readbyqxmd.com/read/29136792/comparative-performances-of-a-bare-graphite-polyurethane-composite-electrode-unmodified-and-modified-with-graphene-and-carbon-nanotubes-in-the-electrochemical-determination-of-escitalopram
#2
Marina Baccarin, Priscila Cervini, Eder Tadeu Gomes Cavalheiro
A bare composite graphite-polyurethane electrode (EGPU) and two other modified with graphene (EGPU-GR) and functionalized multi-walled carbon nanotubes (EGPU-CNTs) were prepared and compared regarding their voltammetric response to escitalopran (EST). The modifiers were characterized by Raman spectroscopy and the resulting electrode materials by contact angle measurements with a hydrophilicity character in the ascending order for the composites: GPU > GPU-GR > GPU-CNTs and scanning electron microscopy (SEM)...
February 1, 2018: Talanta
https://www.readbyqxmd.com/read/29135225/regional-variations-of-spontaneous-transient-adenosine-release-in-brain-slices
#3
Scott T Lee, B Jill Venton
Transient adenosine signaling has been recently discovered in vivo, where the concentration is on average 180 nM and the duration only 3-4 seconds. Characterizing transient adenosine release in brain slices would facilitate pharmacological experiments testing formation and function of adenosine. Here, the goal was to compare the frequency, concentration, and duration of transient events in rat brain slices from the pre-frontal cortex (PFC), hippocampus (CA1), and thalamus using fast-scan cyclic voltammetry...
November 14, 2017: ACS Chemical Neuroscience
https://www.readbyqxmd.com/read/29109253/a-transient-dopamine-signal-encodes-subjective-value-and-causally-influences-demand-in-an-economic-context
#4
Scott A Schelp, Katherine J Pultorak, Dylan R Rakowski, Devan M Gomez, Gregory Krzystyniak, Raibatak Das, Erik B Oleson
The mesolimbic dopamine system is strongly implicated in motivational processes. Currently accepted theories suggest that transient mesolimbic dopamine release events energize reward seeking and encode reward value. During the pursuit of reward, critical associations are formed between the reward and cues that predict its availability. Conditioned by these experiences, dopamine neurons begin to fire upon the earliest presentation of a cue, and again at the receipt of reward. The resulting dopamine concentration scales proportionally to the value of the reward...
November 6, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/29090830/a-novel-biosensor-with-high-signal-to-noise-ratio-for-real-time-measurement-of-dopamine-levels-in-vivo
#5
Akimasa Ishida, Atsushi Imamura, Yoshitomo Ueda, Takeshi Shimizu, Ryosuke Marumoto, Cha-Gyun Jung, Hideki Hida
Fast-scan cyclic voltammetry (FSCV) is an established method for measuring dopamine (DA) levels in the brain in real time. However, it is difficult to discriminate DA from other monoamines such as serotonin (5-hydroxytryptamine, 5-HT) and norepinephrine (NE). We report a novel DA-specific biosensor consisting of a carbon-fiber electrode coated with an ion-exchange membrane, a layer containing monoamine oxidase B, and a cellulose membrane. We performed FSCV using the probe to monitor the amount of DA in vitro and in vivo...
November 1, 2017: Journal of Neuroscience Research
https://www.readbyqxmd.com/read/29068793/use-of-fast-scan-cyclic-voltammetry-to-assess-phasic-dopamine-release-in-rat-models-of-early-postpartum-maternal-behavior-and-neglect
#6
Tatiana A Shnitko, Kyla D Mace, Kaitlin M Sullivan, W Kyle Martin, Elizabeth H Andersen, Sarah K Williams Avram, Josephine M Johns, Donita L Robinson
Maternal behavior (MB) is a complex response to infant cues, orchestrated by postpartum neurophysiology. Although mesolimbic dopamine contributes toward MB, little is known about real-time dopamine fluctuations during the postpartum period. Thus, we used fast-scan cyclic voltammetry to measure individual dopamine transients in the nucleus accumbens of early postpartum rats and compared them with dopamine transients in virgins and in postpartum females exposed to cocaine during pregnancy, which is known to disrupt MB...
December 2017: Behavioural Pharmacology
https://www.readbyqxmd.com/read/29066851/interaction-between-the-trace-amine-associated-receptor-1-and-the-dopamine-d2-receptor-controls-cocaine-s-neurochemical-actions
#7
Aman Asif-Malik, Marius C Hoener, Juan J Canales
Recent evidence suggests that the trace amine-associated receptor 1 (TAAR1) plays a pivotal role in the regulation of dopamine (DA) transmission and cocaine's actions. However, the underlying mechanisms through which TAAR1 activation mediates these effects have not yet been elucidated. Here, we used fast-scan cyclic voltammetry to measure DA dynamics and explore such mechanisms. We show, first, that the full TAAR1 agonist, RO5256390, dose-dependently blocked cocaine-induced inhibition of DA clearance in slices of the nucleus accumbens...
October 24, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29063091/a-baseline-drift-detrending-technique-for-fast-scan-cyclic-voltammetry
#8
Mark DeWaele, Yoonbae Oh, Cheonho Park, Yu Min Kang, Hojin Shin, Charles D Blaha, Kevin E Bennet, In Young Kim, Kendall H Lee, Dong Pyo Jang
Fast scan cyclic voltammetry (FSCV) has been commonly used to measure extracellular neurotransmitter concentrations in the brain. Due to the unstable nature of the background currents inherent in FSCV measurements, analysis of FSCV data is limited to very short amounts of time using traditional background subtraction. In this paper, we propose the use of a zero-phase high pass filter (HPF) as the means to remove the background drift. Instead of the traditional method of low pass filtering across voltammograms to increase the signal to noise ratio, a HPF with a low cutoff frequency was applied to the temporal dataset at each voltage point to remove the background drift...
October 24, 2017: Analyst
https://www.readbyqxmd.com/read/29056522/electrochemical-quantification-of-some-water-soluble-vitamins-in-commercial-multi-vitamin-using-poly-amino-acid-caped-by-graphene-quantum-dots-nanocomposite-as-dual-signal-amplification-elements
#9
Nasrin Shadjou, Mohammad Hasanzadeh, Ali Omari
Rapid analyses of some water soluble vitamins (Vitamin B2, B9, and C) in commercial multi vitamins could be routinely performed in analytical laboratories. This study reports on the electropolymerization of a low toxic and biocompatible polymer "poly aspartic acid-graphene quantum dots" as a novel strategy for surface modification of glassy carbon electrode and preparation a new interface for measurement of selected vitamins in commercial multi vitamins. Electrochemical deposition, as a well-controlled synthesis procedure, has been used for subsequently layer-by-layer preparation of graphene quantum dots nanostructures on a poly aspartic acid using cyclic voltammetry techniques in the regime of -1...
October 19, 2017: Analytical Biochemistry
https://www.readbyqxmd.com/read/29054857/diazepam-concurrently-increases-the-frequency-and-decreases-the-amplitude-of-dopamine-transient-release-events-in-the-nucleus-accumbens
#10
Scott A Schelp, Zachary D Brodnik, Dylan R Rakowski, Katherine J Pultorak, Asha T Sambells, Rodrigo A Espana, Erik B Oleson
Benzodiazepines are commonly prescribed anxiolytics that pose abuse liability in susceptible individuals. While it is well established that all drugs of abuse increase brain dopamine levels and benzodiazepines are allosteric modulators of the GABAAR, it remains unclear how they alter dopamine release. Using in vivo fast-scan cyclic voltammetry, we measured diazepam (trade name valium) induced changes in the frequency and amplitude of transient dopamine release events. We found that diazepam concurrently increase the frequency and decreases the amplitude of transient dopamine release events in the awake and freely-moving rat...
October 20, 2017: Journal of Pharmacology and Experimental Therapeutics
https://www.readbyqxmd.com/read/29030431/distinctive-modulation-of-dopamine-release-in-the-nucleus-accumbens-shell-mediated-by-dopamine-and-acetylcholine-receptors
#11
Jung Hoon Shin, Martin F Adrover, Veronica A Alvarez
Nucleus accumbens (NAc) shell shows unique dopamine (DA) signals in vivo and plays a unique role in DA dependent behaviors, such as reward-motivated learning and the response to drugs of abuse. A di-synaptic mechanism for DA release was reported and shown to require synchronized firing of cholinergic interneurons (CINs) and activation of nicotinic acetylcholine (ACh) receptors (nAChRs) in DA neuron (DAN) axons. The properties of this di-synaptic mechanism of DA transmission is not well understood in the NAc shell...
October 13, 2017: Journal of Neuroscience: the Official Journal of the Society for Neuroscience
https://www.readbyqxmd.com/read/29024860/facile-synthesis-n-doped-hollow-carbon-spheres-from-spherical-solid-silica
#12
K Wenelska, A Ottmann, D Moszyński, P Schneider, R Klingeler, E Mijowska
Nitrogen-doped core/shell carbon nanospheres (NHCS are prepared and their capability as an anode material in lithium-ion batteries is investigated. The synthesis methodology is based on a fast template route. The resulting molecular nanostructures are characterized by X-ray diffraction, transmission electron microscopy, thermal analysis, and nitrogen adsorption/desorption measurement as well as by cyclic voltammetry and galvanostatic cycling. The core/shell structure provides a rapid lithium transport pathway and boasts a highly reversible capacity...
October 5, 2017: Journal of Colloid and Interface Science
https://www.readbyqxmd.com/read/28971565/hypocretin-receptor-1-knockdown-in-the-ventral-tegmental-area-attenuates-mesolimbic-dopamine-signaling-and-reduces-motivation-for-cocaine
#13
David L Bernstein, Preeti S Badve, Jessica R Barson, Caroline E Bass, Rodrigo A España
The hypocretin receptor 1 (HCRTr1) is a critical participant in the regulation of motivated behavior. Previous observations demonstrate that acute pharmacological blockade of HCRTr1 disrupts dopamine (DA) signaling and the motivation for cocaine when delivered systemically or directly into the ventral tegmental area (VTA). To further examine the involvement of HCRTr1 in regulating reward and reinforcement processing, we employed an adeno-associated virus to express a short hairpin RNA designed to knock down HCRTr1...
October 2, 2017: Addiction Biology
https://www.readbyqxmd.com/read/28965353/corticosterone-regulates-both-naturally-occurring-and-cocaine-induced-dopamine-signaling-by-selectively-decreasing-dopamine-uptake
#14
Daniel S Wheeler, Amanda L Ebben, Beliz Kurtoglu, Marissa E Lovell, Austin T Bohn, Isabella A Jasek, David A Baker, John R Mantsch, Paul J Gasser, Robert A Wheeler
Stressful and aversive events promote maladaptive reward-seeking behaviors such as drug addiction by acting, in part, on the mesolimbic dopamine system. Using animal models, data from our laboratory and others show that stress and cocaine can interact to produce a synergistic effect on reward circuitry. This effect is also observed when the stress hormone corticosterone is administered directly into the nucleus accumbens (NAc), indicating that glucocorticoids act locally in dopamine terminal regions to enhance cocaine's effects on dopamine signaling...
October 1, 2017: European Journal of Neuroscience
https://www.readbyqxmd.com/read/28949036/subthalamic-nucleus-and-globus-pallidus-interna-influence-firing-of-tonically-active-neurons-in-the-primate-striatum-through-different-mechanisms
#15
Asuka Nakajima, Yasushi Shimo, Takanori Uka, Nobutaka Hattori
Both the subthalamic nucleus (STN) and the globus pallidus pars interna (GPi) are major targets for neuromodulation therapy for movement disorders. An example of such a therapy is deep brain stimulation (DBS). The striatum is the primary target for pharmacological treatment of these disorders. To further our understanding of both the functional relationships among motor nuclei and the mechanisms of therapies for movement disorders, it is important to clarify how changing the neuronal activity of one target, either by medication or by artificial electrical stimulation, affects the other connected nuclei...
September 26, 2017: European Journal of Neuroscience
https://www.readbyqxmd.com/read/28943284/evaluating-oral-flavorant-effects-on-nicotine-self-administration-behavior-and-phasic-dopamine-signaling
#16
Robert J Wickham, Eric J Nunes, Shannon Hughley, Phillip Silva, Sofia N Walton, Jinwoo Park, Nii A Addy
Understanding how tobacco product flavor additives, such as flavorants in electronic cigarettes, influence smoking behavior and addiction is critical for informing public health policy decisions regarding tobacco product regulation. Here, we developed a combined intraoral (i.o.) and intravenous (i.v.) self-administration paradigm in rats to determine how flavorants influence self-administration behavior. By combining i.o. flavorant delivery with fast scan cyclic voltammetry (FSCV) or i.v. nicotine self-administration in adult, male rats, we examined whether flavors alter phasic dopamine (DA) signaling and nicotine self-administration...
September 21, 2017: Neuropharmacology
https://www.readbyqxmd.com/read/28930994/measuring-in-vivo-changes-in-extracellular-neurotransmitters-during-naturally-rewarding-behaviors-in-female-syrian-hamsters
#17
Kelsey M Moore, Brett T Himmler, Benjamin A Teplitzky, Matthew D Johnson, Robert L Meisel
The ability to measure neurotransmitter release on a rapid time scale allows patterns of neurotransmission to be linked to specific behaviors or manipulations; a powerful tool in elucidating underlying mechanisms and circuitry. While the technique of microdialysis has been used for decades to measure nearly any analyte of interest in the brain, this technique is limited in temporal resolution. Alternatively, fast scan cyclic voltammetry is both temporally precise and extremely sensitive; however, because this technically difficult method relies on the electroactivity of the analyte of interest, the possibility to detect nonelectroactive substances (e...
September 12, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/28918253/deep-brain-stimulation-of-the-medial-forebrain-bundle-elevates-striatal-dopamine-concentration-without-affecting-spontaneous-or-reward-induced-phasic-release
#18
Marianne Klanker, Matthijs Feenstra, Ingo Willuhn, Damiaan Denys
Deep brain stimulation (DBS) of the medial forebrain bundle (MFB) induces rapid improvement of depressive symptoms in patients suffering from treatment-refractory major depressive disorder (MDD). It has been hypothesized that activation of the dopamine (DA) system contributes to this effect. To investigate whether DBS in the MFB affects DA release in the striatum, we combined DBS with fast-scan cyclic voltammetry (FSCV) in freely moving rats. Animals were implanted with a stimulating electrode at the border of the MFB and the ventral tegmental area, and a FSCV microelectrode in the ventromedial striatum to monitor extracellular DA during the acute onset of DBS and subsequent continued stimulation...
November 19, 2017: Neuroscience
https://www.readbyqxmd.com/read/28911630/nonenzymatic-glucose-sensor-based-on-disposable-pencil-graphite-electrode-modified-by-copper-nanoparticles
#19
Sima Pourbeyram, Khadijeh Mehdizadeh
A nonenzymatic glucose sensor based on a disposable pencil graphite electrode (PGE) modified by copper nanoparticles [Cu(NP)] was prepared for the first time. The prepared Cu(NP) exhibited an absorption peak centered at ∼562 nm using UV-visible spectrophotometry and an almost homogenous spherical shape by scanning electron microscopy. Cyclic voltammetry of Cu(NP)-PGE showed an adsorption controlled charge transfer process up to 90.0 mVs(-1). The sensor was applied for the determination of glucose using an amperometry technique with a detection limit of [0...
October 2016: Journal of Food and Drug Analysis
https://www.readbyqxmd.com/read/28889259/an-ultrasensitive-and-selective-electrochemical-sensor-for-determination-of-estrone-3-sulfate-sodium-salt-based-on-molecularly-imprinted-polymer-modified-carbon-paste-electrode
#20
Han Song, Yuli Wang, Lu Zhang, Liping Tian, Jun Luo, Na Zhao, Yajie Han, Feilang Zhao, Xue Ying, Yingchun Li
A highly sensitive and selective electrochemical sensor based on carbon paste electrode (CPE) modified with molecularly imprinted polymers (MIPs) has been developed for the determination of estrone 3-sulfate sodium salt (ESS). MIPs were prepared in polar medium via bulk polymerization and characterized by scanning electron microscopy and infrared spectroscopy. Cyclic voltammetry was performed to the study preparation process and binding behavior of the MIP-modified CPE (MIP/CPE) toward ESS. The conditions for preparing MIPs and MIP/CPE as well as ESS detection were optimized...
September 9, 2017: Analytical and Bioanalytical Chemistry
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