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https://www.readbyqxmd.com/read/28892497/improved-dop-pcr-idop-pcr-a-robust-and-simple-wga-method-for-efficient-amplification-of-low-copy-number-genomic-dna
#1
Konstantin A Blagodatskikh, Vladimir M Kramarov, Ekaterina V Barsova, Alexey V Garkovenko, Dmitriy S Shcherbo, Andrew A Shelenkov, Vera V Ustinova, Maria R Tokarenko, Simon C Baker, Tatiana V Kramarova, Konstantin B Ignatov
Whole-genome amplification (WGA) techniques are used for non-specific amplification of low-copy number DNA, and especially for single-cell genome and transcriptome amplification. There are a number of WGA methods that have been developed over the years. One example is degenerate oligonucleotide-primed PCR (DOP-PCR), which is a very simple, fast and inexpensive WGA technique. Although DOP-PCR has been regarded as one of the pioneering methods for WGA, it only provides low genome coverage and a high allele dropout rate when compared to more modern techniques...
2017: PloS One
https://www.readbyqxmd.com/read/28885202/reference-range-and-variability-of-laser-doppler-fluxmetry
#2
C Mrowietz, R P Franke, G Pindur, U Wolf, F Jung
The Laser Doppler technique, a noninvasive method to estimate skin blood flow (LDF), is frequently used in research and clinical routine [1]. Here, the measurements were carried out with a new Laser Doppler system, the DOPsystem, which allows to measure frequency spectra in four different frequency windows according to the velocities in venules (low velocity), capillaries (low to medium velocities), and in arteries (with high and very high velocities). However, the diagnostic reliability or the effectiveness of the LDF has not yet been evaluated sufficiently, which is indispensable, where medical diagnostics and therapy controls are concerned...
September 4, 2017: Clinical Hemorheology and Microcirculation
https://www.readbyqxmd.com/read/28884722/-severe-carotid-stenosis-cerebral-autoregulation-in-the-ipsilateral-region
#3
V B Semenyutin, G А Asaturyan, А А Nikiforova, G К Panuntsev, V A Aliev, V B Iblyaminov, A V Savello, А Patzak, K V Laptev, I P Dudanov, О А Pavlov
AIM: To study cerebral autoregulation (CA) in region of the stenotic carotid artery. MATERIAL AND METHODS: The study involved 35 patients with critical stenosis of the carotid arteries, including 24 patients were asymptomatic and 11 patients with symptomatic course. Blood flow velocity in middle cerebral arteries was monitored using Multi Dop X (DWL, Germany) with simultaneous noninvasive systemic blood pressure registration (CNAP, Austria). CA was assessed by calculating the phase shift (PS) between spontaneous oscillations of blood flow velocity and blood pressure within the range of systemic Mayer waves (80-120 mHz)...
2017: Zhurnal Nevrologii i Psikhiatrii Imeni S.S. Korsakova
https://www.readbyqxmd.com/read/28870843/phosphatidylserine-a-cancer-cell-targeting-biomarker
#4
REVIEW
Bhupender Sharma, Shamsher S Kanwar
Cancer is a leading cause of mortality and morbidity globally. Many prominent cancer-associated molecules have been identified over the recent years which include EGFR, CD44, TGFbRII, HER2, miR-497, NMP22, BTA, Fibrin/FDP etc. These biomarkers are often used for screening, detection, diagnosis, prognosis, prediction and monitoring of cancer development. Phosphatidylserine (PS) is an essential component in all human cells which is present on the inner leaflet of the cell membrane. The oxidative stress causes exposure of PS on the surface of the vascular endothelium in the cancer cells (lung, breast, pancreatic, bladder, skin, brain metastasis, rectal adenocarcinoma etc...
September 1, 2017: Seminars in Cancer Biology
https://www.readbyqxmd.com/read/28864205/the-delta-opioid-receptor-agonist-knt-127-in-the-prelimbic-medial-prefrontal-cortex-attenuates-veratrine-induced-anxiety-like-behaviors-in-mice
#5
Akiyoshi Saitoh, Satoshi Suzuki, Akinobu Soda, Masanori Ohashi, Misa Yamada, Jun-Ichiro Oka, Hiroshi Nagase, Mitsuhiko Yamada
We previously reported that systemic administration of the selective delta opioid receptor (DOP) agonist KNT-127 produces potent anxiolytic-like effects in rats. Although a higher distribution pattern of DOPs was reported in the prelimbic medial prefrontal cortex (PL-PFC) of rodents, the role of DOPs in PL-PFC and in anxiolytic-like effects have not been well examined. Recently, we demonstrated that activation of PL-PFC with the sodium channel activator veratrine increases glutamatergic neurotransmission and produces anxiety-like behaviors in mice...
August 31, 2017: Behavioural Brain Research
https://www.readbyqxmd.com/read/28856827/transcriptomic-and-physiological-analyses-of-the-dinoflagellate-karenia-mikimotoi-reveal-non-alkaline-phosphatase-based-molecular-machinery-of-atp-utilization
#6
Hao Luo, Xin Lin, Ling Li, Lingxiao Lin, Chao Zhang, Senjie Lin
The ability to utilize dissolved organic phosphorus (DOP) is important for phytoplankton to survive the scarcity of dissolved inorganic phosphorus (DIP), and alkaline phosphatase (AP) has been the major research focus as a facilitating mechanism. Here we employed a unique molecular ecological approach and conducted a broader search for underpinning molecular mechanisms of adenosine triphosphate (ATP) utilization. Cultures of the dinoflagellate Karenia mikimotoi were set up in L1 medium (+P), DIP-depleted L1 medium (-P), and ATP-replacing-DIP medium (ATP)...
August 30, 2017: Environmental Microbiology
https://www.readbyqxmd.com/read/28855588/morphine-activation-of-mu-opioid-receptors-causes-disinhibition-of-neurons-in-the-ventral-tegmental-area-mediated-by-%C3%AE-arrestin2-and-c-src
#7
Fiona A Bull, Daniel T Baptista-Hon, Jeremy J Lambert, Wendy Walwyn, Tim G Hales
The tyrosine kinase, c-Src, participates in mu opioid receptor (MOP) mediated inhibition in sensory neurons in which β-arrestin2 (β-arr2) is implicated in its recruitment. Mice lacking β-arr2 exhibit increased sensitivity to morphine reinforcement; however, whether β-arr2 and/or c-Src participate in the actions of opioids in neurons within the reward pathway is unknown. It is also unclear whether morphine acts exclusively through MOPs, or involves delta opioid receptors (DOPs). We examined the involvement of MOPs, DOPs, β-arr2 and c-Src in the inhibition by morphine of GABAergic inhibitory postsynaptic currents (IPSCs) recorded from neurons in the mouse ventral tegmental area...
August 30, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28843691/dietary-supplementation-with-dried-olive-pomace-in-dairy-cows-modifies-the-composition-of-fatty-acids-and-the-aromatic-profile-in-milk-and-related-cheese
#8
F Castellani, A Vitali, N Bernardi, E Marone, F Palazzo, L Grotta, G Martino
This study aimed to evaluate the effect of dietary integration of dried olive pomace (DOP), a by-product of olive oil separation, on nutritional and aromatic properties of milk and cheese. Twenty dairy cows were divided into 2 groups that were balanced for milk yield, parity, and days in milk. The control group was fed a conventional diet (20 kg of dry matter/head per day), whereas the experimental group (EG) received the conventional diet supplemented with DOP as 10% of dry matter. During the trial, milk yield was recorded and the samples of milk, cheese, total mixed rations, and DOP were collected and analyzed to determine the chemical-nutritional composition and aromatic profile...
August 23, 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/28839284/a-strategy-for-nonmigrating-highly-plasticized-pvc
#9
Jun Yuan, Bin Cheng
Nonmigrating highly plasticized PVC was prepared based on a new compound that acts as a plasticizer that was derived from di(2-ethylhexyl) 4-hydrophthalate and chlorinated paraffin-52. The as-prepared PVC has a plasticizing efficiency as high as DOP and its migration is totally suppressed. Unlike other reported methods, this approach increases the interaction between phthalate and PVC to suppress its migration, not simply to enlarge its molecular size (or molecular weight). This methodology is highly versatile for producing the desired non-leaching PVC with a permanent plasticizer effect...
August 24, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28832014/terahertz-infrared-electrodynamics-of-single-wall-carbon-nanotube-films
#10
Elena S Zhukova, Artem K Grebenko, Anton V Bubis, Anatolii S Prokhorov, Mikhail A Belyanchikov, Alexey P Tsapenko, Evgenia Gilshteyn, Daria S Kopylova, Yury G Gladush, Anton S Anisimov, Vladimir B Anzin, Albert Nasibulin, Boris P Gorshunov
Broad-band (4 to 20 000 cm<sup>-1</sup>) spectra of real and imaginary conductance of a set of high-quality pristine and AuCl<sub>3</sub>-dopped single-walled carbon nanotube (SWCNT) films with different transparency are systematically measured. It is shown that while the high-energy (≥1 eV) response is determined by well-known interband transitions, the lower-energy electrodynamic properties of the films are fully dominated by unbound charge carriers. Their main spectral effect is seen as the free-carrier Drude-type contribution...
August 23, 2017: Nanotechnology
https://www.readbyqxmd.com/read/28818796/dispersion-induced-ozone-pretreatment-of-waste-activated-biosolids-arriving-biomethanation-modelling-parameters-energetic-and-cost-assessment
#11
R Yukesh Kannah, S Kavitha, J Rajesh Banu, Obulisamy Parthiba Karthikeyan, P Sivashanmugham
In this study, the phase separated effect of dispersion induced ozone pretreatment (DOP) was investigated. Solid reduction, biomass lysis and biomethane production were used as essential parameters to assess the potential of DOP over ozone pretreatment (OP). A higher suspended solid reduction of about 25.2% was achieved in DOP than OP 18%. The ozone dosage of 0.014gO3/g SS supported a maximal biomass lysis of about 32.8% when the biosolids were subjected to prior dispersion at 30s and 3000rpm. However, the same ozone dosage without phase separation achieved 9...
November 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28804148/critical-care-pharmacist-market-perceptions-comparison-of-critical-care-program-directors-and-directors-of-pharmacy
#12
David R Hager, Rosemary A Persaud, Ryan W Naseman, Kavish Choudhary, Kristen E Carter, Amanda Hansen
Background: While hospital beds continue to decline as patients previously treated as inpatients are stabilized in ambulatory settings, the number of critical care beds available in the United States continues to rise. Growth in pharmacy student graduation, postgraduate year 2 critical care (PGY2 CC) residency programs, and positions has also increased. There is a perception that the critical care trained pharmacist market is saturated, yet this has not been evaluated since the rise in pharmacy graduates and residency programs...
May 2017: Hospital Pharmacy
https://www.readbyqxmd.com/read/28804044/real-time-ultrasound-doppler-enhances-precision-in-image-guided-approaches-to-the-cerebello-pontine-angle
#13
Davit Baghdasaryan, Marcel Albrecht, Mihr Shahnazaryan, Steffen Rosahl
OBJECTIVE: To evaluate efficacy and reliability of intraoperative Doppler sonography in localizing the transverse and sigmoid sinuses during lateral suboccipital craniotomy. METHODS: A 16-Mhz intraoperative micro-Doppler ultrasound (16Mhz, Multi-Dop pro®, Compumedics, Germany) was applied to detect the medial border of the sigmoid sinus and the inferior border of the transverse sinus in 25 patients. Micro-Doppler measurements were compared to MR- and CT-based image-guidance (Kolibri®, Brainlab, Germany)...
August 10, 2017: World Neurosurgery
https://www.readbyqxmd.com/read/28802292/even-minimal-polishing-of-an-indian-parboiled-brown-rice-variety-leads-to-increased-glycemic-responses
#14
Shanmugam Shobana, Nagarajan Lakshmipriya, Mookambika Ramya Bai, Rajagopal Gayathri, Vaidya Ruchi, Vasudevan Sudha, Nagappa G Malleshi, Kamala Krishnaswamy, C-J K Henry, Ranjit Mohan Anjana, Ranjit Unnikrishnan, Viswanathan Mohan
BACKGROUND AND OBJECTIVES: To evaluate the effect of polishing on the glycemic properties of Indian parboiled brown rice. METHODS AND STUDY DESIGN: We evaluated the effect of different degrees of polishing on the glycemic and insulinemic responses of Bapatla (BPT-5204), Indian parboiled Indica rice variety. Brown rice (BR), under milled rice (UMR) and white rice (WR) with 2.3% and 9.7% degree of polishing (DOP), respectively, were prepared and evaluated for the glycemic properties...
2017: Asia Pacific Journal of Clinical Nutrition
https://www.readbyqxmd.com/read/28762066/zeta-potential-a-case-study-of-cationic-anionic-and-neutral-liposomes
#15
Mackensie C Smith, Rachael M Crist, Jeffrey D Clogston, Scott E McNeil
Zeta potential is often used to approximate a nanoparticle's surface charge, i.e., cationic, anionic, or neutral character, and has become a standard characterization technique to evaluate nanoparticle surfaces. While useful, zeta potential values provide only very general conclusions about surface charge character. Without a thorough understanding of the measurement parameters and limitations of the technique, these values can become meaningless. This case study attempts to explore the sensitivity of zeta potential measurement using specifically formulated cationic, anionic, and neutral liposomes...
July 31, 2017: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/28755722/molecular-mechanism-of-glucose-6-phosphate-utilization-in-the-dinoflagellate-karenia-mikimotoi
#16
Chao Zhang, Hao Luo, Liangmin Huang, Senjie Lin
Phosphorus (P) is an essential nutrient for marine phytoplankton as for other living organisms, and the preferred form, dissolved inorganic phosphate (DIP), is often quickly depleted in the sunlit layer of the ocean. Phytoplankton have developed mechanisms to utilize organic forms of P (DOP). Hydrolysis of DOP to release DIP by alkaline phosphatase is believed to be the most common mechanism of DOP utilization. Little effort has been made, however, to understand other potential molecular mechanisms of utilizing different types of DOP...
July 2017: Harmful Algae
https://www.readbyqxmd.com/read/28747000/response-of-soil-dissolved-organic-matter-to-microplastic-addition-in-chinese-loess-soil
#17
Hongfei Liu, Xiaomei Yang, Guobin Liu, Chutao Liang, Sha Xue, Hao Chen, Coen J Ritsema, Violette Geissen
Plastic debris is accumulating in agricultural land due to the increased use of plastic mulches, which is causing serious environmental problems, especially for biochemical and physical properties of the soil. Dissolved organic matter (DOM) plays a central role in driving soil biogeochemistry, but little information is available on the effects of plastic residues, especially microplastic, on soil DOM. We conducted a soil-incubation experiment in a climate-controlled chamber with three levels of microplastic added to loess soil collected from the Loess Plateau in China: 0% (control, CK), 7% (M1) and 28% (M2) (w/w)...
October 2017: Chemosphere
https://www.readbyqxmd.com/read/28702673/unexpected-thermal-annealing-effects-on-the-viscosity-of-polymer-nanocomposites
#18
Fei Chen, Koshu Takatsuji, Dan Zhao, Xuanji Yu, Sanat K Kumar, Ophelia K C Tsui
The effects of thermal annealing, 12-50 K above the glass transition temperature, on the zero-shear viscosity, η, of polymer nanocomposites (PNCs) and the corresponding host polymers were studied. For all specimens, including neat and 4 wt% dioctyl phthalate (DOP)-plasticized polystyrene (PS), neat poly(methyl methacrylate) (PMMA), and PNCs containing bare and grafted silica nanoparticles in neat and DOP-plasticized PS, the η increased with time initially, and only asymptotically approached a steady-state value after thermal annealing for ∼100 to ∼200 h...
August 9, 2017: Soft Matter
https://www.readbyqxmd.com/read/28699350/analgesic-properties-of-opioid-nk1-multitarget-ligands-with-distinct-in-vitro-profiles-in-naive-and-chronic-constriction-injury-mice
#19
Joanna Starnowska, Roberto Costante, Karel Guillemyn, Katarzyna Popiolek-Barczyk, Nga N Chung, Carole Lemieux, Attila Keresztes, Joost Van Duppen, Adriano Mollica, John Streicher, Jozef Vanden Broeck, Peter W Schiller, Dirk Tourwé, Joanna Mika, Steven Ballet, Barbara Przewlocka
The lower efficacy of opioids in neuropathic pain may be due to the increased activity of pronociceptive systems such as substance P. We present evidence to support this hypothesis in this work from the spinal cord in a neuropathic pain model in mice. Biochemical analysis confirmed the elevated mRNA and protein level of pronociceptive substance P, the major endogenous ligand of the neurokinin-1 (NK1) receptor, in the lumbar spinal cord of chronic constriction injury (CCI)-mice. To improve opioid efficacy in neuropathic pain, novel compounds containing opioid agonist and neurokinin 1 (NK1) receptor antagonist pharmacophores were designed...
July 26, 2017: ACS Chemical Neuroscience
https://www.readbyqxmd.com/read/28694507/a-novel-highly-sensitive-no2-sensor-based-on-perovskite-na0-5-xbi0-5tio3-%C3%AE-electrolyte
#20
Yihong Xiao, Chufan Zhang, Xu Zhang, Guohui Cai, Yong Zheng, Ying Zheng, Fulan Zhong, Lilong Jiang
NOx is one of dangerous air pollutants, and the demands for reliable sensors to detect NOx are extremely urgent recently. Conventional fluorite-phase YSZ used for NOx sensor requires higher operating temperature to obtain desirable oxygen ion conductivity. In this work, perovskite-phase Na0.5Bi0.5TiO3 (NBT) oxygen conductor was chosen as the solid electrolyte to fabricate a novel highly sensitive NO2 sensor with CuO as the sensing electrode and Pt as reference electrode. Na dopped Na0.5Bi0.5TiO3 greatly improved the sensing performance of this sensor...
July 10, 2017: Scientific Reports
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