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https://www.readbyqxmd.com/read/28223451/mycobacterium-tuberculosis-proteasome-accessory-factor-a-pafa-can-transfer-prokaryotic-ubiquitin-like-protein-pup-between-substrates
#1
Susan Zhang, Kristin E Burns-Huang, Guido V Janssen, Huilin Li, Huib Ovaa, Lizbeth Hedstrom, K Heran Darwin
The protein degradation machinery of Mycobacterium tuberculosis includes a proteasome and a ubiquitin-like protein (Pup). Proteasome accessory factor A (PafA) attaches Pup to proteins to target them for degradation by the proteasome. Free Pup is unstable and never observed in extracts of M. tuberculosis, an observation that led us to hypothesize that PafA may need alternative sources of Pup. Here, we show that PafA can move Pup from one proteasome substrate, inositol 1-phosphate synthetase (Ino1), to two different proteins, malonyl coenzyme A (CoA)-acyl carrier protein transacylase (FabD) and lonely guy (Log)...
February 21, 2017: MBio
https://www.readbyqxmd.com/read/28213383/development-of-a-direct-observation-of-procedural-skills-rubric-for-fourth-year-pharmacy-students-in-ambulatory-care-rotations
#2
Sara J Linedecker, Jamie Barner, Jennifer Ridings-Myhra, Aida Garza, Debra Lopez, William McIntyre
PURPOSE: The development and implementation of a pilot program requiring direct observation of procedural skills (DOPS) assessments of fourth-year pharmacy students during ambulatory care rotations are described. METHODS: All fourth-year pharmacy students at the University of Texas at Austin College of Pharmacy who engaged in a required advanced pharmacy practice experience (APPE) in ambulatory care during the period January-May 2015 participated in a DOPS pilot program...
March 1, 2017: American Journal of Health-system Pharmacy: AJHP
https://www.readbyqxmd.com/read/28192197/characterization-of-3-h-oxymorphone-binding-sites-in-mouse-brain-quantitative-autoradiography-in-opioid-receptor-knockout-mice
#3
Ji Hoon Yoo, Anna Borsodi, Géza Tóth, Sándor Benyhe, Robert Gaspar, Audrey Matifas, Brigitte L Kieffer, Athanasios Metaxas, Ian Kitchen, Alexis Bailey
Oxymorphone, one of oxycodone's metabolic products, is a potent opioid receptor agonist which is thought to contribute to the analgesic effect of its parent compound and may have high potential abuse liability. Nonetheless, the in vivo pharmacological binding profile of this drug is still unclear. This study uses mice lacking mu (MOP), kappa (KOP) or delta (DOP) opioid receptors as well as mice lacking all three opioid receptors to provide full characterisation of oxymorphone binding sites in the brain. Saturation binding studies using [(3)H]oxymorphone revealed high affinity binding sites in mouse brain displaying Kd of 1...
February 10, 2017: Neuroscience Letters
https://www.readbyqxmd.com/read/28190408/net-mineral-requirements-of-dairy-goats-during-pregnancy
#4
C J Härter, L D Lima, D S Castagnino, H O Silva, F O M Figueiredo, N R St-Pierre, K T Resende, I A M A Teixeira
Mineral requirements of pregnant dairy goats are still not well defined; therefore, we investigated the net Ca, P, Mg, Na and K requirements for pregnancy and for maintenance during pregnancy in two separate experiments. Experiment 1 was performed to estimate the net Ca, P, Mg, Na and K requirements in goats carrying single or twin fetuses from 50 to 140 days of pregnancy (DOP). The net mineral requirements for pregnancy were determined by measuring mineral deposition in gravid uterus and mammary gland after comparative slaughter...
February 13, 2017: Animal: An International Journal of Animal Bioscience
https://www.readbyqxmd.com/read/28187970/role-of-dop-on-the-alkaline-phosphatase-activity-of-size-fractionated-plankton-in-coastal-waters-in-the-nw-mediterranean-sea-toulon-bay-france
#5
Gérard Bogé, Magali Lespilette, Dominique Jamet, Jean-Louis Jamet
The particulate material was fractionated into 5 size classes (>90μ, 50-90μ, 6-50μ, 1-6μ, and <1μ). DOP was analysed as easily (DOPh, DOPpa) and less easily hydrolysable compounds (DOPox). Based on Vmax, 94% of the high affinity AP activity was due to <50μ cells and 77% to <1μ cells. 83% of the low affinity activity was due to >90μ cells. The high affinity activities were negatively correlated with DOP for the <50μ classes. These correlations came mostly from DOPox. They were more significant when NO3+NO2 concentrations were high, when DIP concentrations were low and when N/P ratio was >10...
February 7, 2017: Marine Pollution Bulletin
https://www.readbyqxmd.com/read/28185452/organic-nanowire-sio2-core-shell-microlasers-with-highly-polarized-and-narrow-emissions-for-biological-imaging
#6
Changfu Feng, Zhenzhen Xu, Xu Wang, Hongjuan Yang, Lemin Zheng, Hongbing Fu
Development of luminescence probes with polarized and narrow emissions simultaneously is helpful for removing multiply scattered light and enables multiplexing detection, but it remains challenging to use conventional organic dyes, fluorescence proteins, and quantum dots. Here, we demonstrated smart one-dimensional microlaser probes (MLPs) by coating a thin layer of silica shell on the surface of organic nanowires (ONWs) of 1,4-dimethoxy-2,5-di[4'-(methylthio)styryl]benzene (TDSB), namely, ONW@SiO2 core-shell structures...
February 20, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28160361/nonmigrating-equivalent-substitutes-for-pvc-dop-formulations-as-shown-by-a-tg-study-of-pvc-with-covalently-bound-peo-ppo-oligomers
#7
Rodrigo Navarro, Tülin Gacal, Melike Ocakoglu, Carolina García, Carlos Elvira, Alberto Gallardo, Helmut Reinecke
Monoamino functionalized ethylenoxide (EO)/propylenoxide oligomers (Jeffamine) are linked chemically to poly(vinyl chloride) (PVC) using trichlorotriazine chemistry in order to prepare nonmigrating internally plasticized materials. The dependence of the plasticizer efficiency on both the number of anchoring points to the chains and the PVC/plasticizer compatibility is investigated using oligomers of different molecular weight and hydrophilic-hydrophobic balance. Hydrophilic oligomers (containing predominantly EO) of molecular weights between 2000 and 5000 g mol(-1) exhibit excellent plasticizer efficiency, nearly identical to di-2-ethylhexylphthalate (DOP) in conventional PVC/DOP mixtures and may therefore be used as nonmigrating equivalents for DOP...
February 3, 2017: Macromolecular Rapid Communications
https://www.readbyqxmd.com/read/28157852/expression-of-the-degree-of-polarization-based-on-the-geometrical-optics-pbrdf-model
#8
Kai Wang, Jingping Zhu, Hong Liu, Bingzheng Du
An expression of the degree of polarization (DOP) based on the geometrical optics polarimetric bidirectional reflectance distribution function model is presented. In this expression, the DOP is related to the surface roughness and decreases at different reflection angles because diffuse reflection is taken into consideration. A shadowing/masking function introduced into the specular reflection expression makes the DOP values decrease as the angle of incidence or observation approaches grazing. Different kinds of materials were measured to validate the accuracy of this DOP expression...
February 1, 2017: Journal of the Optical Society of America. A, Optics, Image Science, and Vision
https://www.readbyqxmd.com/read/28153495/efficient-internalization-of-tat-peptide-in-zwitterionic-dopc-phospholipid-membrane-revealed-by-neutron-diffraction
#9
Xiaochao Chen, Shutao Liu, Bruno Deme, Viviana Cristiglio, Drew Marquardt, Richard Weller, Pingfan Rao, Yunqiang Wang, Jeremy Bradshaw
The aim of this study is to investigate the interactions between TAT peptides and a neutral DOPC bilayer by using neutron lamellar diffraction. The distribution of TAT peptides and the perturbation of water distribution across the DOPC bilayer were revealed. When compared to our previous study on an anionic DOPC/DOPS bilayer (X. Chen et al., Biochim Biophys Acta. 2013. 1828 (8), 1982-1988), a much deeper insertion of TAT peptides was found in the hydrophobic core of DOPC bilayer at a depth of 6.0Å from the center of the bilayer, a position close to the double bond of fatty acyl chain...
January 31, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28153287/development-of-a-disposable-and-low-cost-electrochemical-sensor-for-dopamine-detection-based-on-poly-pyrrole-3-carboxylic-acid-modified-electrochemically-over-oxidized-pencil-graphite-electrode
#10
Ali Özcan, Salih İlkbaş, Ayça Atılır Özcan
In this study, preparation of a single-use electrochemical sensor for the selective and sensitive determination of dopamine (DOP) was investigated by electrochemical polymerization of pyrrole-3-carboxylic acid on electrochemically over-oxidized pencil graphite electrode (p(P3CA)/EOPGE). Cyclic voltammetry measurements of Fe(CN)6(4-/3-) indicated that the electrochemically over-oxidized PGE (EOPGE) showed superior electron transfer characteristics according to bare PGE. The ionized carboxyl groups found in the structure of poly(pyrrole-3-carboxylic acid) (p(P3CA)) showed high affinity towards positively charged DOP...
April 1, 2017: Talanta
https://www.readbyqxmd.com/read/28147550/characterization-of-the-interface-of-binary-mixed-dopc-dops-liposomes-in-water-the-impact-of-charge-condensation
#11
Cornelis Lütgebaucks, Carlos Macias-Romero, Sylvie Roke
Solutions of liposomes composed of binary mixtures of anionic dioleoylphosphatidylserine (DOPS) and zwitterionic dioleoylphosphatidylcholine (DOPC) are investigated with label-free angle-resolved (AR) second harmonic scattering (SHS) and electrophoretic mobility measurements. The membrane surface potential is extracted from the AR-SHS response. The surface potential changes from -10 to -145 mV with varying DOPS content ( from 0% to 100%) and levels off already at ∼ 10 % DOPS content. The ζ-potential shows the same trend but with a drastically lower saturation value (-44 mV)...
January 28, 2017: Journal of Chemical Physics
https://www.readbyqxmd.com/read/28135799/amyloid-%C3%AE-ion-channels-in-a-membrane-comprising-brain-total-lipid-extracts
#12
Joon Lee, Young Hun Kim, Fernando T Arce, Alan L Gillman, Hyunbum Jang, Bruce L Kagan, Ruth Nussinov, Jerry Yang, Ratnesh Lal
Amyloid β (Aβ) oligomers are the predominant toxic species in the pathology of Alzheimer's disease. The prevailing mechanism for toxicity by Aβ oligomers includes ionic homeostasis destabilization in neuronal cells by forming ion channels. These channel structures have been previously studied in model lipid bilayers. In order to gain further insight into the interaction of Aβ oligomers with natural membrane compositions, we have examined the structures and conductivities of Aβ oligomers in a membrane composed of brain total lipid extract (BTLE)...
February 20, 2017: ACS Chemical Neuroscience
https://www.readbyqxmd.com/read/28124015/effect-of-formative-evaluation-using-direct-observation-of-procedural-skills-in-assessment-of-postgraduate-students-of-obstetrics-and-gynecology-prospective-study
#13
Naina Kumar, Namit Kant Singh, Samar Rudra, Swanand Pathak
INTRODUCTION: Direct Observation of Procedural Skills (DOPS) is a way of evaluating procedural skills through observation in the workplace. The purpose of this study was to assess the role of DOPS in teaching and assessment of postgraduate students and to know the effect of repeated DOPS on improvement of the skills and confidence of the students. METHODS: In both phases, significant difference was observed between the two groups on first DOPS comparison (1st phase: p=0...
January 2017: Journal of Advances in Medical Education & Professionalism
https://www.readbyqxmd.com/read/28119453/depupylase-dop-requires-inorganic-phosphate-in-the-active-site-for-catalysis
#14
Marcel Bolten, Christian Vahlensieck, Colette Lipp, Marc Leibundgut, Nenad Ban, Eilika Weber-Ban
Analogous to eukaryotic ubiquitination, proteins in actinobacteria can be post-translationally modified in a process referred to as pupylation, the covalent attachment of prokaryotic ubiquitin-like protein Pup to lysine side chains of the target protein via an isopeptide bond. Like in eukaryotes, an opposing activity counteracts the modification by specific cleavage of the isopeptide bond formed with Pup. However, the enzymes involved in pupylation and depupylation have evolved independently of ubiquitination and are related to the family of ATP-binding and hydrolyzing carboxylate-amine ligases of the glutamine synthetase type...
January 24, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28106808/anti-fatigue-effects-of-the-unique-polysaccharide-marker-of-dendrobium-officinale-on-balb-c-mice
#15
Wei Wei, Zhi-Peng Li, Tong Zhu, Hau-Yee Fung, Tin-Long Wong, Xin Wen, Dik-Lung Ma, Chung-Hang Leung, Quan-Bin Han
Dendrobium officinale extract shows potent anti-fatigue effects; however, the active substance responsible for these effects remains undetermined. A glucomannan with a huge molecular size of 730 kDa, called DOP, was identified as the unique authentication marker of this expensive herb. DOP exhibited immunomodulating effects on macrophages and lymphocytes in our previous study. Clinical reports also showed that people with fatigue syndrome have a disturbed immune system. Because DOP is the unique and dominant component of D...
January 18, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/28106305/diversity-oriented-peptide-stapling-a-third-generation-cuaac-stapling-and-functionalisation-strategy
#16
Thu Tran Phuong, Christian Ørnbøl Larsen, Tobias Røndbjerg, Martina De Foresta, Micha Ben Achim Kunze, Ales Marek, Jacob Hartvig Løper, Lotte-Emilie Boyhus, Astrid Knuhtsen, Kresten Lindorff-Larsen, Daniel Sejer Pedersen
The introduction of macrocyclic constraints in peptides (peptide stapling) is an important tool within peptide medicinal chemistry for stabilising and pre-organising peptides in a desired conformation. In recent years, the copper-catalysed azide-alkyne cycloaddition (CuAAC) has emerged as a powerful method for peptide stapling. However, to date CuAAC stapling has not provided a simple method for obtaining peptides that are easily diversified further. In the present study, we report a new diversity-oriented peptide stapling (DOPS) methodology based on CuAAC chemistry...
January 20, 2017: Chemistry: a European Journal
https://www.readbyqxmd.com/read/28087277/identification-of-ubact-a-ubiquitin-like-protein-along-with-other-homologous-components-of-a-conjugation-system-and-the-proteasome-in-different-gram-negative-bacteria
#17
Gilad Lehmann, Ronald G Udasin, Ido Livneh, Aaron Ciechanover
Systems analogous to the eukaryotic ubiquitin-proteasome system have been previously identified in Archaea, and Actinobacteria (gram-positive), but not in gram-negative bacteria. Here, we report the bioinformatic identification of a novel prokaryotic ubiquitin-like protein, which we name UBact. The phyletic distribution of UBact covers at least five gram-negative bacterial phyla, including Nitrospirae, Armatimonadetes, Verrucomicroba, Nitrospinae, and Planctomycetes. Additionally, it was identified in seven candidate (uncultured) phyla and one Archaeon...
January 10, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28069393/surface-enhanced-raman-spectroscopy-of-self-assembled-layers-of-lipid-molecules-on-nanostructured-au-and-ag-substrates
#18
Nora Slekiene, Lina Ramanauskaite, Valentinas Snitka
In this work surface enhanced Raman spectroscopy (SERS) has been used for the investigation of the self-assembled layers of lipid molecules (SALLMs) deposited on the nanostructured Au and Ag surfaces. The SALLMs were prepared from one part of 1,2-dioleoyl-sn-glycero-3-phospho-l-serine (DOPS) and four parts of 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC) lipids. The synthesis of Au and Ag SERS substrates was based on the direct gold and silver ions reduction onto HF etched silicon wafers. Au SERS substrates were not suitable for the formation of SALLMs because of the inappropriate contact angle of surface...
January 6, 2017: Chemistry and Physics of Lipids
https://www.readbyqxmd.com/read/28049031/the-efficacy-of-dynorphin-fragments-at-the-%C3%AE%C2%BA-%C3%AE-and-%C3%AE-opioid-receptor-in-transfected-hek-cells-and-in-an-animal-model-of-unilateral-peripheral-inflammation
#19
M Morgan, A Heffernan, F Benhabib, S Wagner, A K Hewavitharana, P N Shaw, P J Cabot
Dynorphin 1-17 is an endogenous peptide that is released at sites of inflammation by leukocytes, binding preferentially to κ-opioid receptors (KOP) to mediate nociception. We have previously shown that dynorphin 1-17 is rapidly biotransformed to smaller peptide fragments in inflamed tissue homogenate. This study aimed to determine the efficacy and potency of selected dynorphin fragments produced in an inflamed environment at the KOP, μ and δ-opioid receptors (MOP and DOP respectively) and in a model of inflammatory pain...
December 31, 2016: Peptides
https://www.readbyqxmd.com/read/27995824/not-all-dietary-diversity-scores-can-legitimately-be-interpreted-as-proxies-of-diet-quality
#20
Eric O Verger, Marie-Claude Dop, Yves Martin-Prével
No abstract text is available yet for this article.
December 20, 2016: Public Health Nutrition
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