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https://www.readbyqxmd.com/read/28229416/human-induced-pluripotent-stem-cell-derived-mesenchymal-stem-cells-as-an-individual-specific-and-renewable-source-of-adult-stem-cells
#1
Glen Lester Sequiera, Sekaran Saravanan, Sanjiv Dhingra
This chapter deals with the employment of human-induced pluripotent stem cells (hiPSCs) as a candidate to differentiate into mesenchymal stem cells (MSCs). This would enable to help establish a regular source of human MSCs with the aim of avoiding the problems associated with procuring the MSCs either from different healthy individuals or patients, limited extraction potentials, batch-to-batch variations or from diverse sources such as bone marrow or adipose tissue. The procedures described herein allow for a guided and ensured approach for the regular maintenance of hiPSCs and their subsequent differentiation into MSCs using the prescribed medium...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28227530/linear-discriminant-analysis-for-symmetric-lifting-recognition-of-skilled-logistic-experts-by-center-of-pressure-trajectory
#2
Hieyong Jeong, Michiko Kido, Yuko Ohno, Hieyong Jeong, Michiko Kido, Yuko Ohno, Michiko Kido, Hieyong Jeong, Yuko Ohno
GOAL: The main purpose of the present study was to propose a recognizing method to analyze characteristics of symmetric lifting of skilled logistic experts by center of pressure (CoP) trajectories. Although it has been known that good posture helps reduce the intradiscal loads on lumbar discs, the most significant problem was that most of logistic workers did not know whether the current posture was proper or not. METHODS: The experiment of lifting was performed three times under 18 kg loads with closed eyes...
August 2016: Conference Proceedings: Annual International Conference of the IEEE Engineering in Medicine and Biology Society
https://www.readbyqxmd.com/read/28216821/innovative-modified-hair-follicle-harvesting-technique-with-reverse-rake-scalp-elevator-for-lower-occipital-donor-area-in-follicular-unit-extraction-hair-transplantation
#3
Chandrakant Rambhau Gharwade
Follicular unit extraction (FUE) is one of the widely practiced minimally invasive follicular harvesting techniques employed during hair transplantation. FUE technique has an advantage of utilising lower occipital area and supra-auricular region as a safe donor area described by Unger, in addition to the standard occipital donor area used in strip method (follicular unit transplant). Despite its potential advantages such as rapid recovery, minimal scarring and reduced post-operative pain; its widespread acceptance is limited due to various factors in variable contribution like steeper learning curve and potentially higher follicular transection rates (FTRs)...
September 2016: Indian Journal of Plastic Surgery: Official Publication of the Association of Plastic Surgeons of India
https://www.readbyqxmd.com/read/28215494/2d-versus-3d-in-laparoscopic-surgery-by-beginners-and-experts-a-randomized-controlled-trial-on-a-pelvitrainer-in-objectively-graded-surgical-steps
#4
Johannes Spille, Antonia Wenners, Ulrike von Hehn, Nicolai Maass, Ulrich Pecks, Liselotte Mettler, Ibrahim Alkatout
BACKGROUND AND OBJECTIVE: Progress in endoscopic surgery in the past few decades has led to the application of 3-dimensional (3D) procedures in operating rooms. This permits patient- and surgeon-friendly operations and also maximizes the superiority of laparoscopy over laparotomy. In this study, we compare 2-dimensional (2D) and 3D endoscopy techniques with regard to time, efficiency, optics, and handling by users with different degrees of experience at 4 difficulty levels. DESIGN: A randomized controlled trial on a pelvitrainer in objectively graded surgical steps for students and postgraduates...
February 16, 2017: Journal of Surgical Education
https://www.readbyqxmd.com/read/28210337/extraction-of-knowledge-from-the-topographic-attentive-mapping-network-and-its-application-in-skill-analysis-of-table-tennis
#5
Isao Hayashi, Masanori Fujii, Toshiyuki Maeda, Jasmin Leveille, Tokio Tasaka
The Topographic Attentive Mapping (TAM) network is a biologically-inspired classifier that bears similarities to the human visual system. In case of wrong classification during training, an attentional top-down signal modulates synaptic weights in intermediate layers to reduce the difference between the desired output and the classifier's output. When used in a TAM network, the proposed pruning algorithm improves classification accuracy and allows extracting knowledge as represented by the network structure...
January 1, 2017: Journal of Human Kinetics
https://www.readbyqxmd.com/read/28210243/a-basic-guide-to-real-time-pcr-in-microbial-diagnostics-definitions-parameters-and-everything
#6
REVIEW
Petr Kralik, Matteo Ricchi
Real time PCR (quantitative PCR, qPCR) is now a well-established method for the detection, quantification, and typing of different microbial agents in the areas of clinical and veterinary diagnostics and food safety. Although the concept of PCR is relatively simple, there are specific issues in qPCR that developers and users of this technology must bear in mind. These include the use of correct terminology and definitions, understanding of the principle of PCR, difficulties with interpretation and presentation of data, the limitations of qPCR in different areas of microbial diagnostics and parameters important for the description of qPCR performance...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28203604/effect-of-mirrored-views-on-endoscopic-and-arthroscopic-skill-performance
#7
Emanuel Benninger, Christoph Meier, Stefan Wirth, Peter Philipp Koch, Dominik Meyer
BACKGROUND: Arthroscopic procedures may be technically challenging because of impaired vision, limited space, and the 2-dimensional vision of a 3-dimensional structure. Spatial orientation may get more complicated when the camera is pointing toward the surgeon. HYPOTHESIS: Spatial orientation and arthroscopic performance may be improved by simply mirroring the image on the monitor in different configurations regarding the position and orientation of camera and instrument...
February 2017: Orthopaedic Journal of Sports Medicine
https://www.readbyqxmd.com/read/28195962/ebus-stat-subscore-analysis-to-predict-the-efficacy-and-assess-the-validity-of-virtual-reality-simulation-for-ebus-tbna-training-among-experienced-bronchoscopists
#8
Simone Scarlata, Patrizio Palermo, Piero Candoli, Ariela Tofani, Tommasangelo Petitti, Lorenzo Corbetta
BACKGROUND: Linear endobronchial ultrasound transbronchial needle aspiration (EBUS-TBNA) represents a pivotal innovation in interventional pulmonology; determining the best approach to guarantee systematic and efficient training is expected to become a main issue in the forthcoming years. Virtual reality simulators have been proposed as potential EBUS-TBNA training instruments, to avoid unskilled beginners practicing directly in real-life settings. A validated and perfected simulation program could be used before allowing beginners to practice on patients...
February 10, 2017: Journal of Bronchology & Interventional Pulmonology
https://www.readbyqxmd.com/read/28184205/child-computer-interaction-at-the-beginner-stage-of-music-learning-effects-of-reflexive-interaction-on-children-s-musical-improvisation
#9
Anna Rita Addessi, Filomena Anelli, Diber Benghi, Anders Friberg
In this article children's musical improvisation is investigated through the "reflexive interaction" paradigm. We used a particular system, the MIROR-Impro, implemented in the framework of the MIROR project (EC-FP7), which is able to reply to the child playing a keyboard by a "reflexive" output, mirroring (with repetitions and variations) her/his inputs. The study was conducted in a public primary school, with 47 children, aged 6-7. The experimental design used the convergence procedure, based on three sample groups allowing us to verify if the reflexive interaction using the MIROR-Impro is necessary and/or sufficient to improve the children's abilities to improvise...
2017: Frontiers in Psychology
https://www.readbyqxmd.com/read/28168782/physiological-responses-during-the-practice-of-synchronized-swimming-a-systematic-review
#10
REVIEW
Kátia Ponciano, Maria Luiza de Jesus Miranda, Miwako Homma, João Marcelo Queiroz Miranda, Aylton J Figueira Júnior, Cássio De Miranda Meira Júnior, Danilo Sales Bocalini
This study is aimed at analysing the scientific literature related to physiological responses in synchronized swimmers, produced from 2006 to 2016. A systematic review was conducted using electronic databases (Google Scholar, PubMed, SportDiscus, Web Science, Scielo and Scopus) in national and international journals. The articles were selected using the following inclusion criteria: composed samples of synchronized swimmers of both sexes, articles in English, Spanish and Portuguese, published from 2006 to 2016...
February 6, 2017: Clinical Physiology and Functional Imaging
https://www.readbyqxmd.com/read/28168539/assessment-of-residents-readiness-to-perform-lumbar-puncture-a-validation-study
#11
Mikael Johannes Vuokko Henriksen Md, Troels Wienecke Md PhD, Helle Thagesen Md, Rikke Vita Borre Jacobsen Md PhD, Yousif Subhi Md, Charlotte Ringsted Md PhD, Lars Konge Md PhD
BACKGROUND: Lumbar puncture is a common procedure in many specialties. The procedure serves to diagnose life-threatening conditions, often requiring rapid performance. However, junior doctors possess uncertainties regarding performing the procedure and frequently perform below expectations. Hence, proper training and assessment of performance is crucial before entering clinical practice. OBJECTIVE: To develop and collect validity evidence for an assessment tool for lumbar puncture performance, including a standard to determine when trainees are ready for clinical practice...
February 6, 2017: Journal of General Internal Medicine
https://www.readbyqxmd.com/read/28118787/learning-curve-associated-with-an-automated-laparoscopic-suturing-device-compared-with-laparoscopic-suturing
#12
Steven G Leeds, Lizzy Wooley, Ganesh Sankaranarayanan, Yahya Daoud, James Fleshman, Sanket Chauhan
BACKGROUND: Laparoscopic suturing has proved to be a challenging skill to master which may prevent surgical procedures from being started, or completed, in a minimally invasive fashion. The aim of this study is to compare the learning curves between traditional laparoscopic techniques with a novel suturing device. METHODS: In this prospective single blinded nonrandomized controlled crossover study, we recruited 19 general surgery residents ranging from beginner (PGY1-2, n = 12) to advanced beginner (PGY3-5, n = 7)...
January 1, 2017: Surgical Innovation
https://www.readbyqxmd.com/read/28116390/recent-progress-in-cellulose-nanocrystals-sources-and-production
#13
REVIEW
Djalal Trache, M Hazwan Hussin, M K Mohamad Haafiz, Vijay Kumar Thakur
Cellulose nanocrystals, a class of fascinating bio-based nanoscale materials, have received a tremendous amount of interest both in industry and academia owing to its unique structural features and impressive physicochemical properties such as biocompatibility, biodegradability, renewability, low density, adaptable surface chemistry, optical transparency, and improved mechanical properties. This nanomaterial is a promising candidate for applications in fields such as biomedical, pharmaceuticals, electronics, barrier films, nanocomposites, membranes, supercapacitors, etc...
January 24, 2017: Nanoscale
https://www.readbyqxmd.com/read/28113800/computer-aided-classification-of-gastrointestinal-lesions-in-regular-colonoscopy
#14
Pablo Mesejo, Daniel Pizarro, Armand Abergel, Olivier Rouquette, Sylvain Beorchia, Laurent Poincloux, Adrien Bartoli
We have developed a technique to study how good computers can be at diagnosing gastrointestinal lesions from regular (white light and narrow banded) colonoscopic videos compared to two levels of clinical knowledge (expert and beginner). Our technique includes a novel tissue classification approach which may save clinician's time by avoiding chromoendoscopy, a time-consuming staining procedure using indigo carmine. Our technique also discriminates the severity of individual lesions in patients with many polyps, so that the gastroenterologist can directly focus on those requiring polypectomy...
March 29, 2016: IEEE Transactions on Medical Imaging
https://www.readbyqxmd.com/read/28108316/crispr-cas9-in-insects-applications-best-practices-and-biosafety-concerns
#15
REVIEW
Clauvis Nji Tizi Taning, Benigna Van Eynde, Na Yu, Sanyuan Ma, Guy Smagghe
Discovered as a bacterial adaptive immune system, CRISPR/Cas9 (clustered, regularly interspaced, short palindromic repeat/CRISPR associated) is being developed as an attractive tool in genome editing. Due to its high specificity and applicability, CRISPR/Cas9-mediated gene editing has been employed in a multitude of organisms and cells, including insects, for not only fundamental research such as gene function studies, but also applied research such as modification of organisms of economic importance. Despite the rapid increase in the use of CRISPR in insect genome editing, results still differ from each study, principally due to existing differences in experimental parameters, such as the Cas9 and guide RNA form, the delivery method, the target gene and off-target effects...
January 18, 2017: Journal of Insect Physiology
https://www.readbyqxmd.com/read/28097056/rhea-a-transparent-and-modular-r-pipeline-for-microbial-profiling-based-on-16s-rrna-gene-amplicons
#16
Ilias Lagkouvardos, Sandra Fischer, Neeraj Kumar, Thomas Clavel
The importance of 16S rRNA gene amplicon profiles for understanding the influence of microbes in a variety of environments coupled with the steep reduction in sequencing costs led to a surge of microbial sequencing projects. The expanding crowd of scientists and clinicians wanting to make use of sequencing datasets can choose among a range of multipurpose software platforms, the use of which can be intimidating for non-expert users. Among available pipeline options for high-throughput 16S rRNA gene analysis, the R programming language and software environment for statistical computing stands out for its power and increased flexibility, and the possibility to adhere to most recent best practices and to adjust to individual project needs...
2017: PeerJ
https://www.readbyqxmd.com/read/28080097/correction-to-elliott-and-leach-2016
#17
(no author information available yet)
Reports an error in "You must be lying because I don't understand you: Language proficiency and lie detection" by Elizabeth Elliott and Amy-May Leach (Journal of Experimental Psychology: Applied, 2016[Dec], Vol 22[4], 488-499). (The following abstract of the original article appeared in record 2016-59419-006.) We examined the impact of interviewees' language proficiencies on observers' lie detection performance. Observers (N = 132) were randomly assigned to make deception judgments about interviewees (N = 56) from Four proficiency groups (i...
January 12, 2017: Journal of Experimental Psychology. Applied
https://www.readbyqxmd.com/read/28064547/a-systematic-approach-to-autoinflammatory-syndromes-a-spelling-booklet-for-the-beginner
#18
Donato Rigante
Hallmark of autoinflammatory syndromes (AIS) is the periodic recurrence of 'sterile' inflammatory attacks characterized by fever and organ- or tissue-specific inflammation. Basic research projects over the last two decades have boosted our understanding of pathological pathways, mainly involving interleukin (IL)-1 biosynthesis, and also revealed that their dysregulation results from genetically-heterogeneous inborn errors of innate immunity and leads to multiple inflammatory phenotypes. Starting from the evidence of poor response to IL-1 inhibitors of some patients with multi-organ inflammation, further research studies have disclosed a crucial role for nuclear factor (NF)-κB and type I interferon (IFN) in specific AIS...
January 9, 2017: Expert Review of Clinical Immunology
https://www.readbyqxmd.com/read/28047592/su-g-tep1-07-investigation-of-rapidplan-based-plan-quality-for-breast-imrtsimultaneously-integrated-boost
#19
J Wang, W Hu, X Chen, Z Wu
PURPOSE: The aim of this study is to investigate the feasibility of using RapidPlan for breast cancer radiotherapy and to evaluate its performance for planners with different planning experiences. METHODS: A training database was collected with 80 expert plan datasets from patients previously received left breast conserving surgery and IMRT-simultaneously integrated boost radiotherapy. The models were created on the RapidPlan. Five patients from the training database and 5 external patients were used for internal and external validation, respectively...
June 2016: Medical Physics
https://www.readbyqxmd.com/read/28005009/computer-aided-classification-of-gastrointestinal-lesions-in-regular-colonoscopy
#20
Pablo Mesejo, Daniel Pizarro, Armand Abergel, Olivier Rouquette, Sylvain Beorchia, Laurent Poincloux, Adrien Bartoli
We have developed a technique to study how good computers can be at diagnosing gastrointestinal lesions from regular (white light and narrow banded) colonoscopic videos compared to two levels of clinical knowledge (expert and beginner). Our technique includes a novel tissue classification approach which may save clinician's time by avoiding chromoendoscopy, a time-consuming staining procedure using indigo carmine. Our technique also discriminates the severity of individual lesions in patients with many polyps, so that the gastroenterologist can directly focus on those requiring polypectomy...
September 2016: IEEE Transactions on Medical Imaging
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