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Cellular Imaging

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https://www.readbyqxmd.com/read/29793116/patterns-of-invasive-growth-in-malignant-gliomas-the-hippocampus-emerges-as-an-invasion-spared-brain-region
#1
Awais A Mughal, Lili Zhang, Artem Fayzullin, Andres Server, Yuping Li, Yingxi Wu, Rainer Glass, Torstein Meling, Iver A Langmoen, Trygve B Leergaard, Einar O Vik-Mo
BACKGROUND: Widespread infiltration of tumor cells into surrounding brain parenchyma is a hallmark of malignant gliomas, but little data exist on the overall invasion pattern of tumor cells throughout the brain. METHODS: We have studied the invasive phenotype of malignant gliomas in two invasive mouse models and patients. Tumor invasion patterns were characterized in a patient-derived xenograft mouse model using brain-wide histological analysis and magnetic resonance (MR) imaging...
May 21, 2018: Neoplasia: An International Journal for Oncology Research
https://www.readbyqxmd.com/read/29792352/probing-the-settlement-signals-of-amphibalanus-amphitrite
#2
Mado Kotsiri, Maria Protopapa, Gesthimani-Myrto Roumelioti, Athena Economou-Amilli, Eleni K Efthimiadou, Skarlatos G Dedos
To achieve their reproductive potential, barnacles combine tactile exploration of surface structural properties and integration of cellular signals originating from their antennular sensory setae within a developmentally defined, temporally narrow window of settlement opportunity. Behavioural assays with cyprids coupled with biometric analysis of scanning electron microscopy-acquired images in the presence of specific chemical compounds were used to investigate how settlement on a substratum is altered in response to the presence of these compounds...
May 24, 2018: Biofouling
https://www.readbyqxmd.com/read/29792273/heterogeneity-of-renal-cortical-oxygenation-seeing-is-believing
#3
Roger G Evans, Connie P C Ow
The limited spatial and temporal resolution of available methods for quantifying renal tissue oxygen tension is a major impediment to identification of the roles of renal hypoxia in kidney diseases. Intravital phosphorescence lifetime imaging microscopy allows cellular oxygen tension in the renal cortex of live animals to be resolved to the level of individual tubular cross-sections. This paves the way for future investigations of the spatial relationships between cellular hypoxia and pathophysiological events in kidney disease...
June 2018: Kidney International
https://www.readbyqxmd.com/read/29791140/correlative-stimulated-emission-depletion-and-scanning-ion-conductance-microscopy
#4
Philipp Hagemann, Astrid Gesper, Patrick Happel
Correlation microscopy combining fluorescence and scanning probe or electron microscopy is limited to fixed samples due to the sample preparation and nonphysiological imaging conditions required by most probe or electron microscopy techniques. Among the few scanning probe techniques that allow imaging of living cells under physiological conditions, scanning ion conductance microscopy (SICM) has been shown to be the technique that minimizes the impact on the investigated sample. However, combinations of SICM and fluorescence microscopy suffered from the mismatch in resolution due to the limited resolution of conventional light microscopy...
May 25, 2018: ACS Nano
https://www.readbyqxmd.com/read/29790797/curcumin-loaded-redox-responsive-mesoporous-silica-nanoparticles-for-targeted-breast-cancer-therapy
#5
Nana Li, Zhi Wang, Yongtai Zhang, Kai Zhang, Jianxu Xie, Ying Liu, Wansi Li, Nianping Feng
HYPOTHESIS: The antitumor applications of curcumin (CUR) are limited because of its low water solubility, poor stability, and low bioavailability. We developed novel nanocarrier systems for tumour targeting and controlled CUR release and evaluated their therapeutic efficacy. EXPERIMENTS: The surface of mesoporous silica nanoparticles (MSN) was modified with hyaluronan (HA) or polyethyleneimine-folic acid (PEI-FA) via disulfide bonds. The capacity of the resultant nanocarriers (MSN-HA and MSN-PEI-FA, respectively) for CUR delivery was evaluated in a breast cancer cell line and a mouse xenograft model...
May 23, 2018: Artificial Cells, Nanomedicine, and Biotechnology
https://www.readbyqxmd.com/read/29789819/exploring-the-cellular-uptake-and-localisation-of-phosphorescent-rhenium-fac-tricarbonyl-metallosurfactants-as-a-function-of-lipophilicity
#6
Andrew J Hallett, Emeline Placet, Roxane Prieux, Danielle McCafferty, James A Platts, David Lloyd, Marc Isaacs, Anthony J Hayes, Simon J Coles, Mateusz B Pitak, Sarah Marchant, Stephen N Marriott, Rudolf K Allemann, Athanasia Dervisi, Ian A Fallis
A systematic study of the cellular uptake of emissive complexes as a function of their lipophilicity is presented. Here a series of amphiphilic rhenium fac-tricarbonyl bisimine complexes bearing axial substituted imidazole or thiazole ligands, [Re(bpy)(CO)3(ImCnHm)]+ {n = 1 m = 3 (1+), n = 4 m = 9 (2+), n = 8 m = 17 (3+), n = 12 m = 25 (4+), n = 16 m = 33 (5+), n = 2 m = 3 (6+); bpy = 2,2'-bipyridine, Im = imidazole} and [Re(bpy)(CO)3(L)]+ {L = 1-mesitylimidazole, ImMes (7+), 4,5-dimethylthiazole, dmt (8+) and 4-methyl-5-thiazole-ethanol, mte (9+)} is reported...
May 23, 2018: Dalton Transactions: An International Journal of Inorganic Chemistry
https://www.readbyqxmd.com/read/29788891/targeting-sugar-uptake-and-metabolism-for-cancer-identification-and-therapy-an-overview
#7
Marina Tanasova, Vagarshak V Begoyan, Lukasz J Weselinski
Metabolic deregulations have emerged as a cancer characteristic, opening a broad avenue for strategies and tools to target cancer through sugar uptake and metabolism. High expression levels of sugar transporters in cancer cells offered glycoconjugation as an approach to achieve enhanced cellular accumulation of drugs and imaging agents, with the sugar moiety anchoring the bioactive cargo to cancer cells. On the other hand, high demand for sugar nutrients in cancers provided a new avenue to target cancer cells with metabolic or sugar uptake inhibitors to induce cancer cells starvation or death...
May 22, 2018: Current Topics in Medicinal Chemistry
https://www.readbyqxmd.com/read/29786715/cancer-cell-membrane-coated-magnetic-nanoparticles-for-mr-nir-fluorescence-dual-modal-imaging-and-photodynamic-therapy
#8
Jiong Li, Xuandong Wang, Dongye Zheng, Xinyi Lin, Zuwu Wei, Da Zhang, Zhuanfang Li, Yun Zhang, Ming Wu, Xiaolong Liu
Theranostic nanoprobes integrated with dual-modal imaging and therapeutic functions, such as photodynamic therapy (PDT), have exhibited significant potency in cancer treatments due to their high imaging accuracy and non-invasive advantages for cancer elimination. However, biocompatibility and highly efficient accumulation of these nanoprobes in tumor are still unsatisfactory for clinical application. In this study, a photosensitizer -loaded magnetic nanobead with surface further coated with a layer of cancer cell membrane (SSAP-Ce6@CCM) was designed to improve the biocompatibility and cellular uptake and ultimately achieve enhanced MR/NIR fluorescence imaging and PDT efficacy...
May 22, 2018: Biomaterials Science
https://www.readbyqxmd.com/read/29786467/bombesin-functionalized-64-cu-copper-sulfide-nanoparticles-for-targeted-imaging-of-orthotopic-prostate-cancer
#9
Huawei Cai, Fang Xie, Aditi Mulgaonkar, Lihong Chen, Xiankai Sun, Jer-Tsong Hsieh, Fangyu Peng, Rong Tian, Lin Li, Changqiang Wu, Hua Ai
AIM: To synthesize and evaluate the imaging potential of Bom-PEG-[64 Cu]CuS nanoparticles (NPs) in orothotopic prostate tumor. MATERIALS & METHODS: [64 Cu]CuS NPs were synthesized in aqueous solution by 64 CuCl2 and Na2 S reaction. Then PEG linker with or without bombesin peptide were conjugated to the surface of [64 Cu]CuS NPs to produce Bom-PEG-[64 Cu]CuS and PEG-[64 Cu]CuS NPs. These two kinds of NPs were used for testing specific uptake in prostate cancer cells in vitro and imaging of orthotopic prostate tumor in vivo...
May 22, 2018: Nanomedicine
https://www.readbyqxmd.com/read/29786414/uncovering-the-design-principle-of-amino-acid-derived-photoluminescent-bio-dots-with-tailored-made-structure-properties-and-application-for-cellular-bioimaging
#10
Hesheng Victor Xu, Xinting Zheng, Yanli Zhao, Yen Nee Tan
Natural amino acids possess side chains with different functional groups (R groups), which make them excellent precursors for programmable synthesis of biomolecule-derived nanodots (bio-dots) with desired properties. Herein, we reported the first systematic study to uncover the material design rules of bio-dots synthesis from 20 natural α-amino acids via a green hydrothermal approach. The as-synthesized amino acids bio-dots (AA-dots) are comprehensively characterised to establish a structure-properties relationship between the amino acid precursors and the corresponding photoluminescent properties of AA-dots...
May 22, 2018: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/29785968/force-activatable-coating-enables-high-resolution-cellular-force-imaging-directly-on-regular-cell-culture-surfaces
#11
Anwesha Sarkar, Yuanchang Zhao, Yongliang Wang, Xuefeng Wang
Integrin-transmitted cellular forces are crucial mechanical signals regulating a vast range of cell functions. Although various methods have been developed to visualize and quantify cellular forces at the cell-matrix interface, a method with high performance and low technical barrier is still in demand. Here we developed force-activatable coating (FAC) which can be simply coated on regular cell culture apparatus's surfaces by physical adsorption, and turn these surfaces to force reporting platforms that enable cellular force mapping directly by fluorescence imaging...
May 22, 2018: Physical Biology
https://www.readbyqxmd.com/read/29785469/early-detection-of-cerebral-infarction-after-focal-ischemia-using-a-new-mri-indicator
#12
Yukako Nakajo, Qiang Zhao, Jun-Ichiro Enmi, Hidehiro Iida, Jun C Takahashi, Hiroharu Kataoka, Keiko Yamato, Hiroji Yanamoto
Prolongation of the T2 relaxation time, an increase in T2-weighted signal intensity (T2-SI), and a decrease in the apparent diffusion coefficient (ADC) calculated from diffusion-weighted images (DWI) on magnetic resonance imaging (MRI) are conventional indicators of the vasogenic (interstitial) or cytotoxic (cellular) cerebral edema that develops after ischemic stroke. However, these parameters obtained on stroke imaging have not given us a precise threshold at which we can determine the viability or vulnerability of the tissue, allowing us to decide on an intervention that will help reversible tissue in the acute phase...
May 21, 2018: Molecular Neurobiology
https://www.readbyqxmd.com/read/29785434/integration-of-phospholipid-hyaluronic-acid-methotrexate-nanocarrier-assembly-and-amphiphilic-drug-drug-conjugate-for-synergistic-targeted-delivery-and-combinational-tumor-therapy
#13
Yang Li, Huabing Zhang, Yilin Chen, Jinyuan Ma, Jinyan Lin, Yinying Zhang, Zhongxiong Fan, Guanghao Su, Liya Xie, Xuan Zhu, Zhenqing Hou
Combinational cancer therapy has been considered as a promising strategy to achieve synergetic therapeutic effects and suppression of multidrug resistance. Herein, we adopted a combination of methotrexate (MTX), an antimetabolite acting on cytoplasm, and 10-hydroxycamptothecin (HCPT), an alkaloid acting on nuclei, to treat cancer. Given the different solubilities, membrane permeabilities, and anticancer mechanisms of both drugs, we developed a dual-targeting delivery system based on 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-hyaluronic acid (a principal ligand of CD44 receptors)-MTX (a selective ligand of folate receptors) nanoparticles, which was exploited to carry HCPT-MTX conjugate for synergistically boosting dual-drug co-delivery...
May 22, 2018: Biomaterials Science
https://www.readbyqxmd.com/read/29784761/slc15a2-and-slc15a4-mediate-the-transport-of-bacterially-derived-di-tripeptides-to-enhance-the-nucleotide-binding-oligomerization-domain-dependent-immune-response-in-mouse-bone-marrow-derived-macrophages
#14
Yongjun Hu, Feifeng Song, Huidi Jiang, Gabriel Nuñez, David E Smith
There is increasing evidence that proton-coupled oligopeptide transporters (POTs) can transport bacterially derived chemotactic peptides and therefore reside at the critical interface of innate immune responses and regulation. However, there is substantial contention regarding how these bacterial peptides access the cytosol to exert their effects and which POTs are involved in facilitating this process. Thus, the current study proposed to determine the (sub)cellular expression and functional activity of POTs in macrophages derived from mouse bone marrow and to evaluate the effect of specific POT deletion on the production of inflammatory cytokines in wild-type, Pept2 knockout and Pht1 knockout mice...
May 21, 2018: Journal of Immunology: Official Journal of the American Association of Immunologists
https://www.readbyqxmd.com/read/29784380/fluorescence-activation-imaging-of-localization-distribution-and-level-of-mirna-in-various-organelles-inside-cells
#15
Chuanli Wu, Xiaoyan Liu, Yiyi Zheng, Wenjing He, Guancao Yang, Ping Wu, Chenxin Cai
This work reports an approach for imaging the localization, distribution, and level of miRNA in different organelles based on an activated fluorescence signal triggered by an alteration of the specific binding-induced conformation of the designed activatable probe. We selected miR-150 as an miRNA example to image its localization, distribution, and level in human cervical cancer cells (HeLa cells). The results indicate that miR-150 is localized and distributed in different subcellular organelles (mainly in mitochondria and lysosomes) and that its levels (actually its concentrations) in lysosomes are higher than those in mitochondria in both HeLa and MCF-7 cells...
August 15, 2018: Talanta
https://www.readbyqxmd.com/read/29782304/role-of-in-vivo-reflectance-confocal-microscopy-in-the-analysis-of-melanocytic-lesions
#16
Elena-Daniela Serban, Francesca Farnetani, Giovanni Pellacani, Maria Magdalena Constantin
Worldwide melanoma incidence and mortality are increasing (1). Despite the ongoing research, advanced melanoma is still incurable; therefore, the most appropriate solution seems to be early detection combined with complete surgical excision (2). Since the diagnostic protocol of suspicious lesions includes a complete excision with safety margins (2), the problem of unnecessary scarring is significant. The real challenge in this case is to have a properly formulated diagnosis before acquiring a biopsy. Currently available non-invasive techniques are coherence tomography, digital dermoscopy, and reflectance confocal microscopy...
April 2018: Acta Dermatovenerologica Croatica: ADC
https://www.readbyqxmd.com/read/29782020/whole-mount-immunofluorescence-and-follicle-quantification-of-cultured-mouse-ovaries
#17
Vera D Rinaldi, Jordana C Bloom, John C Schimenti
Research in the field of mammalian reproductive biology often involves evaluating the overall health of ovaries and testes. Specifically, in females, ovarian fitness is often assessed by visualizing and quantifying follicles and oocytes. Because the ovary is an opaque three-dimensional tissue, traditional approaches require laboriously slicing the tissue into numerous serial sections in order to visualize cells throughout the entire organ. Furthermore, because quantification by this method typically entails scoring only a subset of the sections separated by the approximate diameter of an oocyte, it is prone to inaccuracy...
May 2, 2018: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/29781999/preparation-of-murine-submandibular-salivary-gland-for-upright-intravital-microscopy
#18
Xenia Ficht, Flavian Thelen, Bettina Stolp, Jens V Stein
The submandibular salivary gland (SMG) is one of the three major salivary glands, and is of interest for many different fields of biological research, including cell biology, oncology, dentistry, and immunology. The SMG is an exocrine gland comprised of secretory epithelial cells, myofibroblasts, endothelial cells, nerves, and extracellular matrix. Dynamic cellular processes in the rat and mouse SMG have previously been imaged, mostly using inverted multi-photon microscope systems. Here, we describe a straightforward protocol for the surgical preparation and stabilization of the murine SMG in anesthetized mice for in vivo imaging with upright multi-photon microscope systems...
May 7, 2018: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/29781988/automated-slide-scanning-and-segmentation-in-fluorescently-labeled-tissues-using-a-widefield-high-content-analysis-system
#19
Candice C Poon, Vincent Ebacher, Katherine Liu, Voon Wee Yong, John James Patrick Kelly
Automated slide scanning and segmentation of fluorescently-labeled tissues is the most efficient way to analyze whole slides or large tissue sections. Unfortunately, many researchers spend large amounts of time and resources developing and optimizing workflows that are only relevant to their own experiments. In this article, we describe a protocol that can be used by those with access to a widefield high-content analysis system (WHCAS) to image any slide-mounted tissue, with options for customization within pre-built modules found in the associated software...
May 3, 2018: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/29781959/involvement-of-the-vgf-derived-peptide-tlqp-62-in-nerve-injury-induced-hypersensitivity-and-spinal-neuroplasticity
#20
Alexander G J Skorput, Xijing Zhang, Jonathan J Waataja, Cristina D Peterson, Maureen S Riedl, Kelley F Kitto, Hai Truong, Cecilia Huffman, Stephen R Salton, Carolyn A Fairbanks, Christopher N Honda, Lucy Vulchanova
Neuroplasticity in the dorsal horn following peripheral nerve damage contributes critically to the establishment of chronic pain. The neurosecretory protein VGF (non-acronymic) is rapidly and robustly upregulated following nerve injury, and therefore peptides generated from it are positioned to serve as signals for peripheral damage. The goal of this project was to understand the spinal modulatory effects of the C-terminal VGF derived peptide TLQP-62 at the cellular level and gain insight into the function of the peptide in the development of neuropathic pain...
May 16, 2018: Pain
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