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Cellular Imaging

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https://www.readbyqxmd.com/read/29151166/t2-weighted-images-are-superior-to-other-mr-image-types-for-the-determination-of-diffuse-intrinsic-pontine-glioma-intratumoral-heterogeneity
#1
Stephen Harward, S Harrison Farber, Michael Malinzak, Oren Becher, Eric M Thompson
PURPOSE: Diffuse intrinsic pontine glioma (DIPG) remains the main cause of death in children with brain tumors. Given the inefficacy of numerous peripherally delivered agents to treat DIPG, convection enhanced delivery (CED) of therapeutic agents is a promising treatment modality. The purpose of this study was to determine which MR imaging type provides the best discrimination of intratumoral heterogeneity to guide future stereotactic implantation of CED catheters into the most cellular tumor regions...
November 18, 2017: Child's Nervous System: ChNS: Official Journal of the International Society for Pediatric Neurosurgery
https://www.readbyqxmd.com/read/29150867/near-infrared-diffuse-reflectance-signals-for-monitoring-spreading-depolarizations-and-progression-of-the-lesion-in-a-male-rat-focal-cerebral-ischemia-model
#2
Satoko Kawauchi, Izumi Nishidate, Hiroshi Nawashiro, Shunichi Sato
In ischemic stroke research, a better understanding of the pathophysiology and development of neuroprotection methods are crucial, for which in vivo imaging to monitor spreading depolarizations (SDs) and evolution of tissue damage is desired. Since these events are accompanied by cellular morphological changes, light-scattering signals, which are sensitive to cellular and subcellular morphology, can be used for monitoring them. In this study, we performed transcranial imaging of near-infrared (NIR) diffuse reflectance at ∼800 nm, which sensitively reflects light-scattering change, and examined how NIR reflectance is correlated with simultaneously measured cerebral blood flow (CBF) for a rat middle cerebral artery occlusion (MCAO) model...
November 18, 2017: Journal of Neuroscience Research
https://www.readbyqxmd.com/read/29150766/spinobot-an-mri-guided-needle-positioning-system-for-spinal-cellular-therapeutics
#3
Alexander Squires, John N Oshinski, Nicholas M Boulis, Zion Tsz Ho Tse
The neurodegenerative disease amyotrophic lateral sclerosis (ALS) results in the death of motor neurons in voluntary muscles. There are no cures for ALS and few available treatments. In studies with small animal models, injection of cellular therapeutics into the anterior horn of the spinal cord has been shown to inhibit the progression of ALS. It was hypothesized that spinal injection could be made faster and less invasive with the aid of a robot. The robotic system presented-SpinoBot-uses MRI guidance to position a needle for percutaneous injection into the spinal cord...
November 17, 2017: Annals of Biomedical Engineering
https://www.readbyqxmd.com/read/29150629/statistically-strong-label-free-quantitative-identification-of-native-fluorophores-in-a-biological-sample
#4
Saabah B Mahbub, Martin Plöschner, Martin E Gosnell, Ayad G Anwer, Ewa M Goldys
Bioimaging using endogenous cell fluorescence, without any external biomarkers makes it possible to explore cells and tissues in their original native state, also in vivo. In order to be informative, this label-free method requires careful multispectral or hyperspectral recording of autofluorescence images followed by unsupervised extraction (unmixing) of biochemical signatures. The unmixing is difficult due to the scarcity of biochemically pure regions in cells and also because autofluorescence is weak compared with signals from labelled cells, typically leading to low signal to noise ratio...
November 17, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29150182/mature-hippocampal-neurons-require-lis1-for-synaptic-integrity-implications-for-cognition
#5
Anamaria Sudarov, Xin-Jun Zhang, Leighton Braunstein, Eve LoCastro, Shawn Singh, Yu Taniguchi, Ashish Raj, Song-Hai Shi, Holly Moore, M Elizabeth Ross
BACKGROUND: Platelet-activating factor acetylhydrolase 1B1 (LIS1), a critical mediator of neuronal migration in developing brain, is expressed throughout life. However, relatively little is known about LIS1 function in the mature brain. We previously demonstrated that LIS1 involvement in the formation and turnover of synaptic protrusions and synapses of young brain after neuronal migration is complete. Here we examine the requirement for LIS1 to maintain hippocampal circuit function in adulthood...
September 23, 2017: Biological Psychiatry
https://www.readbyqxmd.com/read/29148709/nir-emitting-nanoprobes-based-on-cyclic-rgd-motif-conjugated-pbs-quantum-dots-for-integrin-targeted-optical-bioimaging
#6
Nicoletta Depalo, Michela Corricelli, Ivan de Paola, Gianpiero Valente, Rosa M Iacobazzi, Emiliano Altamura, Doriana Debellis, Daniela Comegna, Elisabetta Fanizza, Nunzio Denora, Valentino Laquintana, Fabio Mavelli, Marinella Striccoli, Michele Saviano, Angela Agostiano, Annarita Del Gatto, Laura Zaccaro, Maria Lucia Curri
Here, silica coated PbS quantum dots (QDs) with photoluminesce emission properties in the near-infrared (NIR) region are proposed as potential effective single particle optical nanoprobes for future in vivo imaging of tumors. The dispersibility in aqueous medium of hydrophobic PbS QDs was accomplished by growing a silica shell on their surface by exploiting a base assisted water-in-oil microemulsion method. The silica coated PbS QDs were then conjugated with specifically designed cyclic arginine-glycine-aspartic acid (cRGD) peptide, that are able to specifically recognizes the αvβ3 integrins which is overexpressed in the angiogenic tumor induced vasculatures and on some solid tumours, in order to achieve a tumor-specific targeting...
November 17, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/29148428/-sup-135-sup-la-as-an-auger-electron-emitter-for-targeted-internal-radiotherapy
#7
Jesper Fonslet, Boon Quan Lee, Thuy A Tran, Mattia Siragusa, Mikael Jensen, Tibor Kibedi, Andrew E Stuchbery, Gregory W Severin
<b>Introduction:</b> <sup>135</sup>La has favorable nuclear and chemical properties for Auger-based targeted internal radiotherapy. Here we present detailed investigations of the production, emissions, imaging characteristics, and dosimetry related to <sup>135</sup>La therapy. <b>Methods and Results:</b> <sup>135</sup>La was produced by 16.5 MeV proton irradiation of metallic <sup>nat</sup>Ba on a medical cyclotron, and was isolated and purified by trap-and-release on weak cation-exchange resin...
November 17, 2017: Physics in Medicine and Biology
https://www.readbyqxmd.com/read/29147537/fluorinated-molecular-beacons-as-functional-dna-nanomolecules-for-cellular-imaging
#8
Cheng Jin, Ting Fu, Ruowen Wang, Hui Liu, Jianmei Zou, Zilong Zhao, Mao Ye, Xiaobing Zhang, Weihong Tan
Molecular beacons (MBs) are simple, but practical, fluorescent nanoprobes widely used to detect small molecules, nucleic acids and proteins. However, some challenges still remain when MBs are employed in complex biological environments, such as instability and non-target interference. To meet such challenges, we have designed and synthesized fluorinated molecular beacons (FMBs) as functional DNA nanomolecules for cellular imaging, in which the stem sequence is simply composed of artificial nucleotides with 3,5-bis(trifluoromethyl)benzene (F) as the surrogate base of natural A, T, C and G bases...
October 1, 2017: Chemical Science
https://www.readbyqxmd.com/read/29147529/ratiometric-photoacoustic-imaging-of-endoplasmic-reticulum-polarity-in-injured-liver-tissues-of-diabetic-mice
#9
Haibin Xiao, Chuanchen Wu, Ping Li, Wen Gao, Wen Zhang, Wei Zhang, Lili Tong, Bo Tang
As one of the complications of diabetes, liver injury results in significant hazards. Therefore, accurately diagnosing diabetes-induced liver injury beforehand is crucial for the warning and treatment of hepatic diseases. Diabetes-induced liver injury can cause changes in the microstructure and morphology of liver tissue, leading to changes in the hydrophilic and hydrophobic domains in the endoplasmic reticulum (ER), which is closely associated with changes in cellular ER polarity. So, differences in the ER polarity can indicate the degree of diabetes-induced liver injury...
October 1, 2017: Chemical Science
https://www.readbyqxmd.com/read/29146920/andy-s-algorithms-new-automated-digital-image-analysis-pipelines-for-fiji
#10
Andrew M K Law, Julia X M Yin, Lesley Castillo, Adelaide I J Young, Catherine Piggin, Samuel Rogers, Catherine Elizabeth Caldon, Andrew Burgess, Ewan K A Millar, Sandra A O'Toole, David Gallego-Ortega, Christopher J Ormandy, Samantha R Oakes
Quantification of cellular antigens and their interactions via antibody-based detection methods are widely used in scientific research. Accurate high-throughput quantitation of these assays using general image analysis software can be time consuming and challenging, particularly when attempted by users with limited image processing and analysis knowledge. To overcome this, we have designed Andy's Algorithms, a series of automated image analysis pipelines for FIJI, that permits rapid, accurate and reproducible batch-processing of 3,3'-diaminobenzidine (DAB) immunohistochemistry, proximity ligation assays (PLAs) and other common assays...
November 16, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29146731/organic-anion-transporting-polypeptide-1b3-as-a-dual-reporter-gene-for-fluorescence-and-magnetic-resonance-imaging
#11
Menq-Rong Wu, Hon-Man Liu, Chen-Wen Lu, Way-Hone Shen, I-Jou Lin, Li-Wen Liao, Yi-You Huang, Ming-Jium Shieh, Jong-Kai Hsiao
Reporter proteins have broad applications in visualizing molecular events at the cellular, tissue and whole-body levels. Transmembrane transporters recognizing specific molecular domains are of particular interest because they enable the migration of signal-source molecules from the extracellular space to the cytoplasm for subsequent application in multimodality imaging. Organic anion-transporting polypeptides (OATPs) have demonstrated their MRI reporter efficacy. We further expanded their use as a dual-modality reporter in MRI and noninvasive in vivo imaging system (IVIS)...
November 16, 2017: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
https://www.readbyqxmd.com/read/29146716/dynamic-contrast-enhanced-mri-derived-intracellular-water-lifetime-%C3%AF-i-a-prognostic-marker-for-patients-with-head-and-neck-squamous-cell-carcinomas
#12
S Chawla, L A Loevner, S G Kim, W-T Hwang, S Wang, G Verma, S Mohan, V LiVolsi, H Quon, H Poptani
BACKGROUND AND PURPOSE: Shutter-speed model analysis of dynamic contrast-enhanced MR imaging allows estimation of mean intracellular water molecule lifetime (a measure of cellular energy metabolism) and volume transfer constant (a measure of hemodynamics). The purpose of this study was to investigate the prognostic utility of pretreatment mean intracellular water molecule lifetime and volume transfer constant in predicting overall survival in patients with squamous cell carcinomas of the head and neck and to stratify p16-positive patients based upon survival outcome...
November 16, 2017: AJNR. American Journal of Neuroradiology
https://www.readbyqxmd.com/read/29145733/harnessing-novel-imaging-approaches-to-guide-hiv-prevention-and-cure-discoveries-a-national-institutes-of-health-and-global-hiv-vaccine-enterprise-2017-meeting-report
#13
Brigitte Elisabeth Sanders-Beer, Yegor Voronin, David McDonald, Anjali Singh
Advances in imaging technologies have greatly increased our understanding of cellular and molecular interactions in humans and their corresponding animal models of infectious diseases. In the HIV/SIV field, imaging has provided key insights into mucosal viral transmission, local and systemic virus spread, host-virus dynamics, and chronic inflammation/immune activation and the resultant immunopathology. Recent developments in imaging applications are yielding physical, spatial, and temporal measurements to enhance insight into biological functions and disease processes, while retaining important cellular, microenvironmental, organ and intact organism contextual details...
November 16, 2017: AIDS Research and Human Retroviruses
https://www.readbyqxmd.com/read/29145487/defining-external-factors-that-determine-neuronal-survival-apoptosis-and-necrosis-during-excitotoxic-injury-using-a-high-content-screening-imaging-platform
#14
Ujval Anilkumar, Petronela Weisova, Jasmin Schmid, Tytus Bernas, Heinrich J Huber, Heiko Düssmann, Niamh M C Connolly, Jochen H M Prehn
Cell death induced by excessive glutamate receptor overactivation, excitotoxicity, has been implicated in several acute and chronic neurological disorders. While numerous studies have demonstrated the contribution of biochemically and genetically activated cell death pathways in excitotoxic injury, the factors mediating passive, excitotoxic necrosis are less thoroughly investigated. To address this question, we developed a high content screening (HCS) based assay to collect high volumes of quantitative cellular imaging data and elucidated the effects of intrinsic and external factors on excitotoxic necrosis and apoptosis...
2017: PloS One
https://www.readbyqxmd.com/read/29145453/the-antiviral-drug-tenofovir-an-inhibitor-of-pannexin-1-mediated-atp-release-prevents-liver-and-skin-fibrosis-by-downregulating-adenosine-levels-in-the-liver-and-skin
#15
Jessica L Feig, Aranzazu Mediero, Carmen Corciulo, Hailing Liu, Jin Zhang, Miguel Perez-Aso, Laura Picard, Tuere Wilder, Bruce Cronstein
BACKGROUND: Fibrosing diseases are a leading cause of morbidity and mortality worldwide and, therefore, there is a need for safe and effective antifibrotic therapies. Adenosine, generated extracellularly by the dephosphorylation of adenine nucleotides, ligates specific receptors which play a critical role in development of hepatic and dermal fibrosis. Results of recent clinical trials indicate that tenofovir, a widely used antiviral agent, reverses hepatic fibrosis/cirrhosis in patients with chronic hepatitis B infection...
2017: PloS One
https://www.readbyqxmd.com/read/29145428/abnormal-brain-white-matter-microstructure-is-associated-with-both-pre-hypertension-and-hypertension
#16
Hideaki Suzuki, He Gao, Wenjia Bai, Evangelos Evangelou, Ben Glocker, Declan P O'Regan, Paul Elliott, Paul M Matthews
OBJECTIVES: To characterize effects of chronically elevated blood pressure on the brain, we tested for brain white matter microstructural differences associated with normotension, pre-hypertension and hypertension in recently available brain magnetic resonance imaging data from 4659 participants without known neurological or psychiatric disease (62.3±7.4 yrs, 47.0% male) in UK Biobank. METHODS: For assessment of white matter microstructure, we used measures derived from neurite orientation dispersion and density imaging (NODDI) including the intracellular volume fraction (an estimate of neurite density) and isotropic volume fraction (an index of the relative extra-cellular water diffusion)...
2017: PloS One
https://www.readbyqxmd.com/read/29144609/internalization-kinetics-and-cytoplasmic-localization-of-functionalized-diatomite-nanoparticles-in-cancer-cells-by-raman-imaging
#17
Stefano Managò, Nunzia Migliaccio, Monica Terracciano, Michela Napolitano, Nicola M Martucci, Luca De Stefano, Ivo Rendina, Anna Chiara De Luca, Annalisa Lamberti, Ilaria Rea
Porous biosilica nanoparticles obtained from diatomites (DNPs) have been recently demonstrated to be non-toxic nanovectors of therapeutic agents in cancer cells. In this work, the internalization kinetics and intracellular spatial distribution of functionalized DNPs incubated with human lung epidermoid carcinoma cell line (H1355) up to 72 h are investigated by Raman imaging. The label-free Raman results are compared with confocal fluorescence microscopy and photoluminescence (PL) data. Raman bands specifically assigned to DNPs and cellular components provide evidence that the nanovectors are internalized and co-localize with lipid environments...
November 16, 2017: Journal of Biophotonics
https://www.readbyqxmd.com/read/29144410/evaluation-of-different-single-walled-carbon-nanotube-surface-coatings-for-single-particle-tracking-applications-in-biological-environments
#18
Zhenghong Gao, Noémie Danné, Antoine Guillaume Godin, Brahim Lounis, Laurent Cognet
Fluorescence imaging of biological systems down to the single-molecule level has generated many advances in cellular biology. For applications within intact tissue, single-walled carbon nanotubes (SWCNTs) are emerging as distinctive single-molecule nanoprobes, due to their near-infrared photoluminescence properties. For this, SWCNT surfaces must be coated using adequate molecular moieties. Yet, the choice of the suspension agent is critical since it influences both the chemical and emission properties of the SWCNTs within their environment...
November 16, 2017: Nanomaterials
https://www.readbyqxmd.com/read/29143844/charged-probes-turn-on-selective-fluorescence-for-rna
#19
Bahareh Shirinfar, Humaira Seema, Nisar Ahmed
RNA controls many biological processes. The selective detection and imaging of RNA molecules can provide information about their location, kinetics, and functions at the cellular level. The imidazolium-based positively charged probes would play a significant role in the deep investigation of RNA biomolecules to check their therapeutic potential and aid in the future rational molecular and drug design.
November 16, 2017: Organic & Biomolecular Chemistry
https://www.readbyqxmd.com/read/29143829/spectral-properties-of-ionic-benzotristhiazole-based-donor-acceptor-nlo-phores-in-polymer-matrices-and-their-one-and-two-photon-cellular-imaging-ability
#20
M Danko, P Hrdlovič, A Martinická, A Benda, M Cigáň
A series of ionic benzotristhiazolium (BTT) push-pull chromophores, with different nitrogen donor groups and different lengths of conjugated bridges, was successfully doped in polar polymer matrices (PVC and PSS). The spectral (photophysical) properties of their low concentration thin polymeric films are compared with those in solution and are discussed in terms of matrix polarity/viscosity influence, specific polymer-chromophore interaction, structure-spectral property relationship and Twisted Intramolecular Charge-Transfer (TICT) state formation...
November 16, 2017: Photochemical & Photobiological Sciences
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