Nurgulsim Kaster, Begenova Ainagul Baibolsynovna, Ijaz Ahmad, Kazhgaliyev Nurlybay Zhigerbayevich, Imbay Seisembay, Akhmetbekov Nurbolat, Shaikenova Kymbat Hamitovna, Omarova Karlygash Mirambekovna, Makhanbetova Aizhan Bekbolatovna, Amangaliyev T G, Ateikhan Bolatbek, Titanov Zhanat Yeginbaevich, Begenova Ainagul Baibolsynovna, Ahmed A El-Mansi, Zan Linsen
OBJECTIVE: The present study was executed to explore the molecular mechanism of FGF10 gene in bovine adipogenesis. METHODS: The bovine FGF10 gene was overexpressed through Ad-FGF10 or inhibited through siFGF10 and their NCs in bovine adipocytes, and the MOI, transfection efficiency, interference efficiency were evaluated through qRT-PCR, western blotting and fluorescence microscopy. The lipid droplets, TG content and the expression levels of adipogenic marker genes were measured during preadipocytes differentiation...
November 1, 2023: Animal bioscience