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https://www.readbyqxmd.com/read/29665894/-effect-of-oct4a-gene-on-the-biological-characteristics-of-k562-cells
#1
Fan-Jing Meng, Jiang Cao, Chong Chen, Qing-Yun Wu, Xu-Guang Song, Wei Chen, Kai-Lin Xu, Wan-Chuan Zhuang
OBJECTIVE: To evaluate biological effects of OCT4A gene on K562 cells and explore the molecular mechanism of K562 cell apoptosis. METHODS: Two recombinant lentiviral vectors were constructed, which could stablely up- regulate and down- regulate OCT4A protein. Recombinant lentivirus was generated by co-transfection of three-plasmids and transfec-ted into K562 cells. The experiments were divided into 5 groups: normal, pLVX-OCT4A-ZsGreen1, pLVX vector control, PLB-OCT4A shRNA and non-specific shRNA groups...
April 2018: Zhongguo Shi Yan Xue Ye Xue za Zhi
https://www.readbyqxmd.com/read/29661186/the-positive-role-of-vitronectin-in-radiation-induced-lung-toxicity-the-in-vitro-and-in-vivo-mechanism-study
#2
Tian-Le Shen, Mi-Na Liu, Qin Zhang, Wen Feng, Wen Yu, Xiao-Long Fu, Xu-Wei Cai
BACKGROUND: Radiation-induced lung toxicity (RILT) is a severe complication of radiotherapy in patients with thoracic tumors. Through proteomics, we have previously identified vitronectin (VTN) as a potential biomarker for patients with lung toxicity of grade ≥ 2 radiation. Herein, we explored the molecular mechanism of VTN in the process of RILT. METHODS: In this study, lentivirus encoding for VTN and VTN-specific siRNA were constructed and transfected into the cultured fibroblasts and C57BL mice...
April 16, 2018: Journal of Translational Medicine
https://www.readbyqxmd.com/read/29660328/antitumor-efficacy-of-vp22-cd-5-fc-suicide-gene-system-mediated-by-lentivirus-in-a-murine-uveal-melanoma-model
#3
Sisi Liu, Wenjie Song, Fusheng Liu, Junwen Zhang, Siquan Zhu
Uveal melanoma (UM) is the most common primary intraocular tumor in adults, which has high frequency of metastasis to the liver, typically causing a fatal outcome. Chemo-resistance remains a major obstacle in the therapeutic approach to UM, leaving limited choice for treating UM. Other possible treatments have been explored but the results are yet to be evident. To improve therapy for UM, transcriptional suicide genes were transfected into the OCM-1 cell line. In the current study, OCM-1 cells transfected with lentiviral-meditated EGFP, cytosine deaminase (CD)/EGFP, and VP22-CD/EGFP were established...
April 13, 2018: Experimental Eye Research
https://www.readbyqxmd.com/read/29659623/loss-of-cxcr6-coreceptor-usage-characterizes-pathogenic-lentiviruses
#4
Katherine S Wetzel, Yanjie Yi, Anjana Yadav, Anya M Bauer, Ezekiel A Bello, Dino C Romero, Frederic Bibollet-Ruche, Beatrice H Hahn, Mirko Paiardini, Guido Silvestri, Martine Peeters, Ronald G Collman
Pandemic HIV-1 originated from the cross-species transmission of SIVcpz, which infects chimpanzees, while SIVcpz itself emerged following the cross-species transmission and recombination of monkey SIVs, with env contributed by the SIVgsn/mus/mon lineage that infects greater spot-nosed, mustached and mona monkeys. SIVcpz and HIV-1 are pathogenic in their respective hosts, while the phenotype of their SIVgsn/mus/mon ancestors is unknown. However, two well-studied SIV infected natural hosts, sooty mangabeys (SMs) and African green monkeys (AGMs), typically remain healthy despite high viral loads; these species express low levels of the canonical coreceptor CCR5, and recent work shows that CXCR6 is a major coreceptor for SIV in these hosts...
April 16, 2018: PLoS Pathogens
https://www.readbyqxmd.com/read/29659618/vitamin-d-receptor-suppresses-proliferation-and-metastasis-in-renal-cell-carcinoma-cell-lines-via-regulating-the-expression-of-the-epithelial-ca2-channel-trpv5
#5
YongMing Chen, XinYu Liu, FaBiao Zhang, ShanFan Liao, XiYuan He, DeXiang Zhuo, HuaiBin Huang, YongYang Wu
We previously demonstrated that transient receptor potential vanilloid subfamily 5 (TRPV5) expression was decreased in renal cell carcinoma (RCC) compared with that in normal kidney tissues, a finding that was correlated with vitamin D receptor (VDR) expression, but further investigations is warranted. The aim of this study was to elucidate whether VDR could regulate the expression of TRPV5 and affect proliferation and metastasis in RCC. In this study, we used lentivirus to conduct the model of VDR overexpression and knockdown caki-1 and 786-O RCC cell lines in vitro...
2018: PloS One
https://www.readbyqxmd.com/read/29659560/androgen-receptor-is-inactivated-and-degraded-in-bladder-cancer-cells-by-phenyl-glucosamine-via-mir-449a-restoration
#6
Ju Guo, Jieping Hu, Runfu Cao, Qingsheng Chen, Kanghua Li
BACKGROUND Bladder cancer caused by exposure to aniline dyes, chronic cystitis, and smoking is detected in approximately 70 000 new cases annually. In the USA alone, it leads to 15 000 deaths every year. In the present study, we investigated the role of 3-((4'-amino-[1,1'-biphenyl]-4-yl)amino)-4-bromo-5-oxo-2,5-dihydrofuran-2-yl acetate (ABDHFA) in the inhibition of bladder cancer cell viability. MATERIAL AND METHODS Viability of cells was examined using MTT assay and distribution of cell cycle was assessed by flow cytometry...
April 16, 2018: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
https://www.readbyqxmd.com/read/29658564/c-terminal-binding-protein%C3%A2-2-mediates-cisplatin-chemoresistance-in-esophageal-cancer-cells-via-the-inhibition-of-apoptosis
#7
Hui Shi, Yinting Mao, Qianqian Ju, Yingcheng Wu, Wen Bai, Peiwen Wang, Yudong Zhang, Maorong Jiang
C-terminal binding protein‑2 (CtBP2) is a transcriptional co-repressor that is associated with tumorigenesis and tumor progression. It has been reported to predict a poor prognosis in several human cancers, including esophageal squamous cell carcinoma (ESCC). The present study aimed to investigate the involvement of CtBP2 in the cisplatin (DDP) resistance of the ECA109 ESCC cell line and its effect on the expression of apoptosis-associated proteins. Constructed recombinant lentiviruses were used for the knockdown or overexpression of CtBP2 in ECA109 cells, and the expression of CtBP2 was measured using reverse transcription-quantitative polymerase chain reaction and western blotting following transfection...
April 12, 2018: International Journal of Oncology
https://www.readbyqxmd.com/read/29657399/seroprevalence-of-small-ruminant-caprine-arthritis-encephalitis-lentivirus-among-goats-from-selected-small-ruminant-farms-in-selangor-malaysia
#8
Faez Firdaus Abdullah Jesse, Asinamai Athliamai Bitrus, Yusuf Abba, Veenosha Nehru Raju, Idris Umar Hambali, Innocent Damudu Peter, Abd Wahid Haron, Mohd Azmi Mohd Lila, Jefri Mohd Norsidin
Background and Aim: Caprine arthritis encephalitis (CAE) is an important viral disease of small ruminants particularly in dairy goats with severe social and economic implication. Hence, this study was designed to determine the seroprevalence of CAE virus (CAEV) among goat population in selected small ruminant farms in Selangor and the risk factors associated with the occurrence of the disease. Materials and Methods: Blood samples were collected from a total of 91 goats selected at random...
February 2018: Veterinary World
https://www.readbyqxmd.com/read/29655789/interleukin-enhanced-binding-factor-2-interacts-with-nlrp3-to-inhibit-the-nlrp3-inflammasome-activation
#9
Jing Jin, Aixin Li, Wenbiao Wang, Jianguo Wu
The activation of the NLRP3 inflammasome is a key process of host immune response that establishes the first line of defense against pathogen infections and cellular stresses, whereas excessive inflammasome activation may damage the hosts, and thus it must be precisely controlled. However, the mechanism underlying the repression of the NLRP3 inflammasome activation remains largely unknown. In this study, by establishing and using a reconstructed NLRP3 inflammasome activation system, we reveal that the NLRP3 inflammasome activation, pro-caspase-1 cleavage, and pro-interleukin-1β (pro-IL-1β) activation are repressed by the interleukin-enhanced binding factor 2 (ILF2)...
April 12, 2018: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29655784/aristolochic-acid-i-interferes-with-the-expression-of-blcap-tumor-suppressor-gene-in-human-cells
#10
Ying-Tzu Huang, Ting-Shuan Wu, Chuan-Chen Lu, Feng-Yih Yu, Biing-Hui Liu
Aristolochic acid I (AAI) is a phytocompound that is linked to the progressive renal disease and development of human urothelial carcinoma. The bladder cancer-associated protein (BLCAP) gene exhibits a tumor suppressor function in various tumors, including bladder carcinoma. This study evaluated the effect of AAI on BLCAP expression and its associated mechanism in human cells. Administering AAI to human embryonic kidney cells (HEK293), human proximal tubule epithelial cells (HK-2) and urinary bladder cancer cells (HT-1376) significantly reduced the expression of BLCAP mRNA and protein...
April 12, 2018: Toxicology Letters
https://www.readbyqxmd.com/read/29653302/jembrana-disease-virus-vif-antagonizes-the-inhibition-of-bovine-apobec3-proteins-through-ubiquitin-mediate-protein-degradation
#11
Xing Su, Hong Wang, Xiaohong Zhou, Zhaolong Li, Baisong Zheng, Wenyan Zhang
Viral infectivity factor (Vif) encoded by lentiviruses is essential for viral replication and escaping from antiviral activity of host defensive factors APOBEC3. Jembrana disease virus (JDV) causes an acute disease syndrome with approximately 20% case fatality rate in Bali cattle. However, the interplay mechanism between JDV Vif and Bos taurus APOBEC3 (btA3) is poorly understood. In this study, we determined that JDV Vif recruits ElonginB, ElonginC(ELOB/C), Cul2 and RBX1 but without the need of CBF-β to form E3 ubiquitin ligase and induces the degradation of btA3 proteins...
April 10, 2018: Virology
https://www.readbyqxmd.com/read/29650696/npy-induces-stress-resilience-via-down-regulation-of-i-h-in-principal-neurons-of-rat-basolateral-amygdala
#12
Heika Silveira Villarroel, Maria Bompolaki, James Mackay, Ana Pamela Miranda Tapia, Sheldon D Michaelson, Randy J Leitermann, Robert A Marr, Janice H Urban, William F Colmers
Neuropeptide Y (NPY) expression is tightly linked with the development of stress resilience in rodents and humans. Local NPY injections targeting the basolateral amygdala (BLA) produce long-term behavioral stress resilience in male rats via an unknown mechanism. Previously, we showed that activation of NPY Y1 receptors hyperpolarizes BLA principal neurons (PNs) through inhibition of the hyperpolarization-activated, depolarizing H-current, Ih The present studies tested whether NPY treatment induces stress resilience by modulating Ih NPY (10 pmol) was delivered daily for 5 days bilaterally into the BLA to induce resilience; thereafter the electrophysiological properties of PNs and the expression of Ih in the BLA were characterized...
April 12, 2018: Journal of Neuroscience: the Official Journal of the Society for Neuroscience
https://www.readbyqxmd.com/read/29649409/the-forkhead-transcription-factor-foxc2-is-required-for-maintaining-murine-spermatogonial-stem-cells
#13
Chao Wei, Hao Lin, Sheng Cui
Continuous spermatogenesis from puberty to old age in males relies on spermatogonial stem cells (SSCs) that possess the property of self-renewal and differentiation. The delicate balance between self-renewal and differentiation is of great importance. In mice, SSCs exist as a subpopulation of undifferentiated spermatogonia. SSCs are controlled by intrinsic molecular pathways which can be activated by extrinsic signals. Our results here first show that the expression of forkhead box C2 (FOXC2) is restricted to GFRα1 positive spermatogonia in the testis...
March 13, 2018: Stem Cells and Development
https://www.readbyqxmd.com/read/29642583/feline-apobec3s-barriers-to-cross-species-transmission-of-fiv
#14
REVIEW
Zeli Zhang, Qinyong Gu, Daniela Marino, Kyeong-Lim Lee, Il-Keun Kong, Dieter Häussinger, Carsten Münk
The replication of lentiviruses highly depends on host cellular factors, which defines their species-specific tropism. Cellular restriction factors that can inhibit lentiviral replication were recently identified. Feline immunodeficiency virus (FIV) was found to be sensitive to several feline cellular restriction factors, such as apolipoprotein B mRNA-editing enzyme, catalytic polypeptide-like 3 (APOBEC3) and tetherin, but FIV evolved to counteract them. Here, we describe the molecular mechanisms by which feline APOBEC3 restriction factors inhibit FIV replication and discuss the molecular interaction of APOBEC3 proteins with the viral antagonizing protein Vif...
April 10, 2018: Viruses
https://www.readbyqxmd.com/read/29630117/urg4-silencing-suppresses-cell-proliferation-in-nasopharyngeal-carcinoma-through-induction-of-apoptosis
#15
X-Y Luo, B Yan, C-F Cai, A-M Chen, M Zhou
OBJECTIVE: To investigate the expression and function of up-regulator of gene-4 (URG4) in nasopharyngeal carcinoma (NPC). PATIENTS AND METHODS: Fresh NPC tumor tissue samples with paired adjacent normal nasopharyngeal tissues samples of 9 NPC patients were collected from NPC curative resection surgery. NPC cell lines (CNE1, CNE2 and HONE1) were cultured. Lentivirus-mediated URG4-specific short hairpin RNA (shRNA) stable transfection was done. The effect of URG4 on CNE4 and HONE1 cells viability was determined via 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT)...
March 2018: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/29629494/cold-stimuli-facilitate-inflammatory-responses-through-transient-receptor-potential-melastatin-8-trpm8-in-primary-airway-epithelial-cells-of-asthmatic-mice
#16
Haipei Liu, Li Hua, Quanhua Liu, Jun Pan, Yixiao Bao
Bronchial asthma is a chronic inflammatory airway disease that can be aggravated by cold air. However, its mechanism remains largely unknown. As a thermo-sensing cation channel, transient receptor potential melastatin 8 (TRPM8) can be activated by cold stimuli (8-22 °C) and cooling agents. Whereas TRPM8 activation leads to enhanced expression of inflammatory cytokines and mucus hypersecretion in human bronchial epithelial cell lines, no previous study has examined its role in regulating the cold-induced inflammatory responses and its mechanism in asthmatic airway epithelium...
April 9, 2018: Inflammation
https://www.readbyqxmd.com/read/29628991/retraction-note-to-lentivirus-mediated-short-hairpin-rna-targeting-protein-phosphatase-4-regulatory-subunit-1-inhibits-growth-in-breast-cancer
#17
Yuying Qi, Tinghui Hu, Kai Lin, Renqing Ye, Zuodong Ye
[This retracts the article on p. 218 in vol. 18, PMID: 26472971.].
March 2018: Journal of Breast Cancer
https://www.readbyqxmd.com/read/29628936/clinical-and-functional-characterization-of-a-missense-elf2-variant-in-a-canvas-family
#18
Hena Ahmad, Teresa Requena, Lidia Frejo, Marien Cobo, Alvaro Gallego-Martinez, Francisco Martin, Jose A Lopez-Escamez, Adolfo M Bronstein
Cerebellar ataxia with neuropathy and bilateral vestibular areflexia syndrome (CANVAS) is a rare disorder with an unknown etiology. We present a British family with presumed autosomal dominant CANVAS with incomplete penetrance and variable expressivity. Exome sequencing identified a rare missense variant in the ELF2 gene at chr4:g.140058846 C > T, c.10G > A, p.A4T which segregated in all affected patients. By using transduced BE (2)-M17 cells, we found that the mutated ELF2 (mt-ELF2) gene increased ATXN2 and reduced ELOVL5 gene expression, the causal genes of type 2 and type 38 spinocerebellar ataxias...
2018: Frontiers in Genetics
https://www.readbyqxmd.com/read/29623778/involvement-of-dopamine-system-in-the-regulation-of-the-brain-corticotropin-releasing-hormone-in-paraventricular-nucleus-in-a-rat-model-of-chronic-visceral-pain
#19
Ning-Ning Ji, Jie Kang, Rong Hua, Yong-Mei Zhang
Objective We aimed to investigate the mechanism of paraventricular nucleus (PVN) and ventral tegmental area (VTA) circuit in the pathogenesis of visceral pain-depression with a rat model induced by neonatal and adult colorectal distension (CRD). Methods Neonate male Sprague-Dayley (SD) rats underwent CRD on postnatal days 8, 10, and 12, and when matured, were tested for adult abdominal withdrawal reflex (AWR) scores to assess visceral hypersensitivity. The forced swimming test was employed to evaluate depression-like behaviors...
April 6, 2018: Neurological Research
https://www.readbyqxmd.com/read/29622770/tnf-%C3%AE-induces-human-neural-progenitor-cell-survival-after-oxygen-glucose-deprivation-by-activating-the-nf-%C3%AE%C2%BAb-pathway
#20
Miri Kim, Kwangsoo Jung, Il-Sun Kim, Il-Shin Lee, Younhee Ko, Jeong Eun Shin, Kook In Park
Neural progenitor cell (NPC) transplantation has been shown to be beneficial in the ischemic brain. However, the low survival rate of transplanted NPCs in an ischemic microenvironment limits their therapeutic effects. Tumor necrosis factor-alpha (TNF-α) is one of the proinflammatory cytokines involved in the pathogenesis of various injuries. On the other hand, several studies have shown that TNF-α influences the proliferation, survival, and differentiation of NPCs. Our study investigated the effect of TNF-α pretreatment on human NPCs (hNPCs) under ischemia-related conditions in vitro...
April 6, 2018: Experimental & Molecular Medicine
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