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https://www.readbyqxmd.com/read/28743112/microrna-302b-3p-suppresses-cell-proliferation-through-akt-pathway-by-targeting-igf-1r-in-human-gastric-cancer
#1
Bo Guo, Zhenghao Zhao, Zhen Wang, Qian Li, Xiaofei Wang, Wenjing Wang, Tusheng Song, Che Huang
BACKGROUND/AIMS: MiR-302b is a major microRNA found in human embryonic stem cells and induced pluripotent stem cells. However, its function in gastric cancer progression remains unclear. METHODS: Quantitative reverse transcription-PCR was performed to detect the expression levels of miR-302b-3p in gastric cancer tissues. MTT, colony formation, and flow cytometer analyses were conducted to explore the function of miR-302b-3p in MKN-45/SGC-7901 cells. A dual-luciferase reporter was used to validate the bioinformatics-predicted target gene of miR-302b-3p...
July 25, 2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/28743103/lanthanum-chloride-inhibits-lps-mediated-expressions-of-pro-inflammatory-cytokines-and-adhesion-molecules-in-huvecs-involvement-of-nf-%C3%AE%C2%BAb-jmjd3-signaling
#2
Xia Chen, Min Xiu, Juanjuan Xing, Shaoqing Yu, Dinghong Min, Fei Guo
BACKGROUND/AIMS: To investigate the regulation of LaCl3 on lipopolysaccharides (LPS)-induced pro-inflammatory cytokines and adhesion molecules in human umbilical vein endothelial cells (HUVECs). METHODS: Primary cultured HUVECs were pretreated with 2.5 µM LaCl3 for 30 min followed by 1 µg/ml LPS for 2 h. Pro-inflammatory cytokine and adhesion molecule expressions were determined by real-time RT-PCR and ELISA. NF-κB/p65 nuclear translocation was examined by immunofluorescence and immuno-blot, and its DNA-binding activity was measured by chemiluminescence...
July 25, 2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/28743062/peripheral-leucocytes-and-tissue-gene-expression-of-granzyme-b-perforin-system-and-serpinb9-impact-on-inflammation-and-insulin-resistance-in-coronary-atherosclerosis
#3
Eman F Sanad, Nadia M Hamdy, Adel K El-Etriby, Samer A Sebak, Hala O El-Mesallamy
AIM: The imbalance between proapoptotic granzyme B (GZB)/perforin (PRF) system and proteinase inhibitor-9 (PI-9; serpinB9); the only known inhibitor of human GZB, has been demonstrated in atherosclerosis. However, their role in atherosclerosis with the impact of type 2 diabetes mellitus (DM) as well as their contribution to hallmarks of atherosclerosis is not clear. SUBJECTS AND METHODS: ELISA for serum insulin, high sensitivity C-reactive protein (hsCRP) and GZB levels in atherosclerotic coronary artery diseases (CAD) patients were estimated in comparison to apparently healthy controls, while GZB, PRF and PI-9 mRNA expression levels were quantified by Taqman RT-PCR in both peripheral leucocytes and atherosclerotic tissues...
July 12, 2017: Diabetes Research and Clinical Practice
https://www.readbyqxmd.com/read/28743044/electrophysiological-and-gene-expression-characterization-of-the-ontogeny-of-nestin-expressing-cells-in-the-adult-mouse-midbrain
#4
Anupama Dey, Parisa Farzanehfar, Elena V Gazina, Tim D Aumann
The birth of new neurons, or neurogenesis, in the adult midbrain is important for progressing dopamine cell-replacement therapies for Parkinson's disease. Most studies suggest newborn cells remain undifferentiated or differentiate into glia within the adult midbrain. However, some studies suggest nestin+neural precursor cells (NPCs) have a propensity to generate new neurons here. We sought to confirm this by administering tamoxifen to adult NesCreER(T2)/R26eYFP transgenic mice, which permanently labelled adult nestin-expressing cells and their progeny with enhanced yellow fluorescent protein (eYFP)...
July 4, 2017: Stem Cell Research
https://www.readbyqxmd.com/read/28743037/exogenous-applications-of-polyamines-modulate-drought-responses-in-wheat-through-osmolytes-accumulation-increasing-free-polyamine-levels-and-regulation-of-polyamine-biosynthetic-genes
#5
Heba Talat Ebeed, Nemat Mohamed Hassan, Alshafei Mohammed Aljarani
Polyamines (PAs) can improve drought stress tolerance in plants; however, very limited information is available on the mechanism of action of exogenous application by different methods under drought stress in wheat. The present study investigates the mechanism through which seed priming and foliar spraying with PAs protect wheat plants from drought stress. 10 days old wheat seedlings were exposed to drought stress by withholding water alone or with 100 μM PAs solutions (putrescine, Put; spermine, Spm; and mixture of Put and Spm for 10 h seed-priming or three foliar sprays during withholding water...
July 15, 2017: Plant Physiology and Biochemistry: PPB
https://www.readbyqxmd.com/read/28743034/therapeutic-advances-in-musculoskeletal-aav-targeting-approaches
#6
REVIEW
Karen Bulaklak, Xiao Xiao
The use of recombinant adeno-associated viruses (rAAVs) is highly prevalent in musculoskeletal gene therapies due to their versatility, high transduction efficiency, natural tropism and vector genome persistence for years. As the largest organ in the body, treatment of skeletal muscle for widespread and sufficient therapeutic gene expression is highly challenging. In addition to disease-specific hurdles, vector genome loss, off-target gene transfer and immune responses to treatment can diminish the overall benefit of rAAV therapies...
July 22, 2017: Current Opinion in Pharmacology
https://www.readbyqxmd.com/read/28742980/disease-relevant-modifications-of-the-methylome-and-transcriptome-by-particulate-matter-pm2-5-from-biomass-combustion
#7
Katharina Heßelbach, Gwang-Jin Kim, Stephan Flemming, Thomas Häupl, Marc Bonin, Regina Dornhof, Stefan Günther, Irmgard Merfort, Matjaz Humar
Exposure to particulate matter (PM) is recognized as a major health hazard, but molecular responses are still insufficiently described. We analyzed the epigenetic impact of ambient PM2.5 from biomass combustion on the methylome of primary human bronchial epithelial BEAS-2B cells using the Illumina HumanMethylation450 BeadChip. The transcriptome was determined by the Affymetrix HG-U133 Plus 2.0 Array. PM2.5 induced genome wide alterations of the DNA methylation pattern, including differentially methylated CpGs in the promoter region associated with CpG islands...
July 25, 2017: Epigenetics: Official Journal of the DNA Methylation Society
https://www.readbyqxmd.com/read/28742942/regulation-of-transposable-elements-interplay-between-te-encoded-regulatory-sequences-and-host-specific-trans-acting-factors-in-drosophila-melanogaster
#8
Ana Marija Jakšić, Robert Kofler, Christian Schlötterer
Transposable elements (TEs) are mobile genetic elements that can move around the genome and their expression is one precondition for this mobility. Because the insertion of TEs in new genomic positions is largely deleterious, the molecular mechanisms for transcriptional suppression have been extensively studied. In contrast, very little is known about their primary transcriptional regulation. Here, we characterize the expression dynamics of TE families in Drosophila melanogaster across a broad temperature range (13-29°C)...
July 25, 2017: Molecular Ecology
https://www.readbyqxmd.com/read/28742940/functional-analysis-of-the-pepper-protein-phosphatase-caaipp1-and-its-interacting-partner-caairf1-modulation-of-aba-signaling-and-the-drought-stress-response
#9
Woonhee Baek, Chae Woo Lim, Sung Chul Lee
Plant adaptive responses to abiotic stress are coordinated by restriction of plant growth and development. The plant hormone abscisic acid (ABA) is the key regulator of the response to abiotic stress, and its sensitivity determines abiotic stress tolerance levels. We previously showed that the E3 ubiquitin ligase CaAIRF1 functions as a positive regulator of ABA and drought stress via modulation of transcription and stability of the type 2C protein phosphatase CaADIP1. Here, we report the identification and functional analysis of a novel type 2C phosphatase, CaAIPP1 (Capsicum annuum CaAIRF1 Interacting Protein Phosphatase 1)...
July 25, 2017: Plant, Cell & Environment
https://www.readbyqxmd.com/read/28742933/gene-expression-is-more-strongly-influenced-by-age-than-caste-in-the-ant-lasius-niger
#10
Eric R Lucas, Jonathan Romiguier, Laurent Keller
Phenotypic plasticity, where a single genome can give rise to different phenotypes, underlies many remarkable features of the natural world and occurs in a wide range of organisms. Understanding the transcriptional differences that underlie plastic phenotypes remains a major unsolved problem in biology. In many ants, females can develop into either queens or workers, two phenotypes with different morphology, behaviour and longevity. In comparison to workers, queens are larger, more fecund and longer-lived. Here, we study gene expression differences between queens and workers in the ant Lasius niger...
July 25, 2017: Molecular Ecology
https://www.readbyqxmd.com/read/28742876/expression-of-uncharacterized-male-germ-cell-specific-genes-and-discovery-of-novel-sperm-tail-proteins-in-mice
#11
Jun Tae Kwon, Sera Ham, Suyeon Jeon, Youil Kim, Seungmin Oh, Chunghee Cho
The identification and characterization of germ cell-specific genes are essential if we hope to comprehensively understand the mechanisms of spermatogenesis and fertilization. Here, we searched the mouse UniGene databases and identified 13 novel genes as being putatively testis-specific or -predominant. Our in silico and in vitro analyses revealed that the expressions of these genes are testis- and germ cell-specific, and that they are regulated in a stage-specific manner during spermatogenesis. We generated antibodies against the proteins encoded by seven of the genes to facilitate their characterization in male germ cells...
2017: PloS One
https://www.readbyqxmd.com/read/28742872/the-wheat-wrky-transcription-factors-tawrky49-and-tawrky62-confer-differential-high-temperature-seedling-plant-resistance-to-puccinia-striiformis-f-sp-tritici
#12
Junjuan Wang, Fei Tao, Wei Tian, Zhongfeng Guo, Xianming Chen, Xiangming Xu, Hongsheng Shang, Xiaoping Hu
WRKY transcription factors (TFs) play crucial roles in plant resistance responses to pathogens. Wheat stripe rust, caused by the fungal pathogen Puccinia striiformis f. sp. tritici (Pst), is a destructive disease of wheat (Triticum aestivum) worldwide. In this study, the two WRKY genes TaWRKY49 and TaWRKY62 were originally identified in association with high-temperature seedling-plant resistance to Pst (HTSP) resistance in wheat cultivar Xiaoyan 6 by RNA-seq. Interestingly, the expression levels of TaWRKY49 and TaWRKY62 were down- and up-regulated, respectively, during HTSP resistance in response to Pst...
2017: PloS One
https://www.readbyqxmd.com/read/28742836/the-impact-of-met-igf-1-igf1r-expression-and-egfr-mutations-on-survival-of-patients-with-non-small-cell-lung-cancer
#13
Samer Al-Saad, Elin Richardsen, Thomas K Kilvaer, Tom Donnem, Sigve Andersen, Mehrdad Khanehkenari, Roy M Bremnes, Lill-Tove Busund
INTRODUCTION: To compare the efficacy of silver in situ hybridization (SISH) and immunohistochemistry (IHC) in detecting MET and IGF1R alterations and to investigate their prevalence and prognostic significance. A possible correlation between MET receptor expression, MET gene alterations and the two most frequent occurring EGFR gene mutations was also investigated. MATERIALS AND METHODS: Stage I to IIIA tumors from 326 patients with NSCLC were immunohistochemically tested for protein expression of MET and IGF-1...
2017: PloS One
https://www.readbyqxmd.com/read/28742830/regulatory-effects-of-zinc-on-cadmium-induced-cytotoxicity-in-chronic-inflammation
#14
Paola Bonaventura, Aline Lamboux, Francis Albarède, Pierre Miossec
OBJECTIVES: Zinc (Zn) has major effects on immune system activation while Cadmium (Cd) has anti-inflammatory and anti-proliferative effects in several chronic inflammatory contexts. The aim of this work was to investigate by which mechanisms Zn could compete with Cd and eventually counteract its deleterious effects. Rheumatoid arthritis (RA) synoviocytes exposed to cytokines were used as a model of chronic inflammation; osteoarthritis (OA) synoviocytes were used as control. METHODS: Cell/medium fractionation constants were analyzed for different metals by inductively-coupled-plasma mass-spectrometry by comparison to the 70Zn spike...
2017: PloS One
https://www.readbyqxmd.com/read/28742823/genome-wide-identification-and-analysis-of-the-mads-box-gene-family-in-bread-wheat-triticum-aestivum-l
#15
Jian Ma, Yujie Yang, Wei Luo, Congcong Yang, Puyang Ding, Yaxi Liu, Linyi Qiao, Zhijian Chang, Hongwei Geng, Penghao Wang, Qiantao Jiang, Jirui Wang, Guoyue Chen, Yuming Wei, Youliang Zheng, Xiujin Lan
The MADS-box genes encode transcription factors with key roles in plant growth and development. A comprehensive analysis of the MADS-box gene family in bread wheat (Triticum aestivum) has not yet been conducted, and our understanding of their roles in stress is rather limited. Here, we report the identification and characterization of the MADS-box gene family in wheat. A total of 180 MADS-box genes classified as 32 Mα, 5 Mγ, 5 Mδ, and 138 MIKC types were identified. Evolutionary analysis of the orthologs among T...
2017: PloS One
https://www.readbyqxmd.com/read/28742792/evidence-for-genetic-association-between-chromosome-1q-loci-and-predisposition-to-colorectal-neoplasia
#16
Stephanie A Schubert, Dina Ruano, Fadwa A Elsayed, Arnoud Boot, Stijn Crobach, Arantza Farina Sarasqueta, Bruce Wolffenbuttel, Melanie M van der Klauw, Jan Oosting, Carli M Tops, Ronald van Eijk, Hans Fa Vasen, Rolf Ham Vossen, Maartje Nielsen, Sergi Castellví-Bel, Clara Ruiz-Ponte, Ian Tomlinson, Malcolm G Dunlop, Pavel Vodicka, Juul T Wijnen, Frederik J Hes, Hans Morreau, Noel Fcc de Miranda, Rolf H Sijmons, Tom van Wezel
BACKGROUND: A substantial fraction of familial colorectal cancer (CRC) and polyposis heritability remains unexplained. This study aimed to identify predisposing loci in patients with these disorders. METHODS: Homozygosity mapping was performed using 222 563 SNPs in 302 index patients with various colorectal neoplasms and 3367 controls. Linkage analysis, exome and whole-genome sequencing were performed in a family affected by microsatellite stable CRCs. Candidate variants were genotyped in 10 554 cases and 21 480 controls...
July 25, 2017: British Journal of Cancer
https://www.readbyqxmd.com/read/28742689/lymphangiogenic-gene-expression-is-associated-with-lymph-node-recurrence-and-poor-prognosis-after-partial-hepatectomy-for-colorectal-liver-metastasis
#17
Thomas T Vellinga, Onno Kranenburg, Nicola Frenkel, Inge Ubink, Dieuwke Marvin, Klaas Govaert, Susanne van Schelven, Jeroen Hagendoorn, Inne H Borel Rinkes
OBJECTIVES: To investigate the relevance of lymphangiogenic gene expression in primary and liver metastasis of colorectal cancer (CRC) and identify determinants of lymphatic invasion. BACKGROUND: Lymphatic development promoting vascular endothelial growth factor C (VEGFC) is associated with poor outcome in primary CRC. For colorectal liver metastasis (CRLM), intrahepatic lymph invasion and lymph node metastasis are poor prognostic factors. Exact biological factors promoting lymphatic involvement remain elusive, just as the association with molecular subtypes of CRC...
July 24, 2017: Annals of Surgery
https://www.readbyqxmd.com/read/28742501/novel-insights-into-the-molecular-mechanisms-underlying-generalized-glucocorticoid-resistance-and-hypersensitivity-syndromes
#18
REVIEW
Nicolas C Nicolaides, Evangelia Charmandari
Glucocorticoids play a fundamental role in many physiologic functions and contribute substantially to the achievement of homeostasis. These pleiotropic glucocorticoid actions are mediated by a ubiquitously expressed transcription factor, the human glucocorticoid receptor (hGR), which may influence the transcription rate of numerous target genes, interact with other transcription factors, trigger the activation of several kinase pathways or modulate mitochondrial DNA expression. Any genetic defects in the NR3C1gene that encodes the hGR may cause Primary Generalized Glucocorticoid Resistance or Hypersensitivity Syndromes, two rare allostatic endocrinologic conditions characterized by partial impaired tissue sensitivity to glucocorticoids...
April 2017: Hormones: International Journal of Endocrinology and Metabolism
https://www.readbyqxmd.com/read/28742387/vitamin-d-loaded-nanostructured-lipid-carrier-nlc-a-new-strategy-for-enhancing-efficacy-of-doxorubicin-in-breast-cancer-treatment
#19
Mehdi Sabzichi, Jamal Mohammadian, Maryam Mohammadi, Farhad Jahanfar, Ali Akbar Movassagh Pour, Hamed Hamishehkar, Alireza Ostad-Rahimi
The dangerous dose-dependent side effects of anticancer agents triggered the finding of new approaches for elevated chemotherapy efficacy. This study investigated the potential application of nanostructured lipid careers (NLCs) in increasing vitamin D3 (VitD) effectiveness in breast cancer cell (MCF-7) in concurrent administration with doxorubicin (Dox). VitD-loaded NLCs were characterized by particle size, zeta potential, Fourier transform infrared spectroscopy, and scanning electron microscope. Cytotoxicity and molecular effects of formulation were evaluated by MTT, DAPI staining, flow cytometry, and real-time quantitative PCR assays...
July 25, 2017: Nutrition and Cancer
https://www.readbyqxmd.com/read/28742368/borapetoside-e-a-clerodane-diterpenoid-extracted-from-tinospora-crispa-improves-hyperglycemia-and-hyperlipidemia-in-high-fat-diet-induced-type-2-diabetes-mice
#20
Yuhui Xu, Yanfen Niu, Yuan Gao, Fang Wang, Wanying Qin, Yanting Lu, Jing Hu, Li Peng, Jikai Liu, Wenyong Xiong
An insidious increase in the incidence of obesity, insulin resistance, and hyperlipidemia has led to an epidemic of type 2 diabetes worldwide. Tinospora crispa (T. crispa) is a familiar plant traditionally used in herbal medicine for diabetes; however, the major active ingredients of this plant are still unclear. In this study, we identified the therapeutic effects of borapetoside E, a small molecule extracted from T. crispa, in high-fat-diet (HFD)-induced obesity in mice. The therapeutic effects of borapetoside E in HFD-induced obese mice were assessed physiologically, histologically, and biochemically following intraperitoneal injection...
July 25, 2017: Journal of Natural Products
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