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Vinculin antibody

Alexander W Peterson, Michael Halter, Alessandro Tona, Anne L Plant, John T Elliott
Surface plasmon resonance microscopy (SPRM) is a powerful label-free imaging technique with spatial resolution approaching the optical diffraction limit. The high sensitivity of SPRM to small changes in index of refraction at an interface allows imaging of dynamic protein structures within a cell. Visualization of subcellular features, such as focal adhesions (FAs), can be performed on live cells using a high numerical aperture objective lens with a digital light projector to precisely position the incident angle of the excitation light...
2018: Proceedings of SPIE
Prahalathan Pichavaram, Wen Yin, Kirk W Evanson, Jonathan H Jaggar, Salvatore Mancarella
Aims: Stromal interaction molecule 1 (STIM1) has emerged as an important player in the regulation of growth and proliferation of smooth muscle cells. Therefore, we hypothesized that STIM1 plays a crucial role in the maintenance of vascular integrity. The objective of this study was to evaluate whether reduced expression of STIM1 could modify the structure and function of the vasculature, leading to changes in blood pressure (BP). Methods and results: Smooth muscle-specific STIM1 knockout (sm-STIM1 KO) in mice resulted in arteries with ∼80% reduced STIM1 protein expression as compared with control mice...
April 1, 2018: Cardiovascular Research
Aristo Vojdani, Elroy Vojdani, Datis Kharrazian
AIM: To evaluate the measurement of zonulin level and antibodies of zonulin and other tight junction proteins in the blood of controls and celiac disease patients. METHODS: This study was conducted to assess the variability or stability of zonulin levels vs IgA and IgG antibodies against zonulin in blood samples from 18 controls at 0, 6, 24 and 30 h after blood draw. We also measured zonulin level as well as zonulin, occludin, vinculin, aquaporin 4 and glial fibrillary acidic protein antibodies in the sera of 30 patients with celiac disease and 30 controls using enzyme-linked immunosorbent assay methodology...
August 21, 2017: World Journal of Gastroenterology: WJG
Michael Friberg Bruun Nielsen, Michael Bau Mortensen, Sönke Detlefsen
Pancreatic stellate cells (PSCs) play a central role as source of fibrogenic cells in pancreatic cancer and chronic pancreatitis. In contrast to quiescent hepatic stellate cells (qHSCs), a specific marker for quiescent PSCs (qPSCs) that can be used in formalin-fixed and paraffin embedded (FFPE) normal human pancreatic tissue has not been identified. The aim of this study was to identify a marker enabling the identification of qPSCs in normal human FFPE pancreatic tissue. Immunohistochemical (IHC), double-IHC, immunofluorescence (IF) and double-IF analyses were carried out using a tissue microarray consisting of cores with normal human pancreatic tissue...
October 2017: Histochemistry and Cell Biology
Ali Rezaie, Sung Chul Park, Walter Morales, Emily Marsh, Anthony Lembo, Jae Hak Kim, Stacy Weitsman, Kathleen S Chua, Gillian M Barlow, Mark Pimentel
BACKGROUND: Antibodies to cytolethal distending toxin B (CdtB) and vinculin are novel biomarkers that rule-in and differentiate irritable bowel syndrome with diarrhea (IBS-D) from other causes of diarrhea and healthy controls. AIM: To determine whether these antibodies can also diagnose and differentiate other IBS subtypes. METHODS: Subjects with IBS-D based on Rome III criteria (n = 2375) were recruited from a large-scale multicenter clinical trial (TARGET 3)...
June 2017: Digestive Diseases and Sciences
M Schmulson, R Balbuena, C Corona de Law
INTRODUCTION: Circulating anti-CdtB/anti-vinculin antibodies have been validated as biomarkers to distinguish IBS-D from IBD, but there is no experience with them in Latin America. MATERIALS AND METHODS: The analysis was carried out on patients seen at a FGIDs/motility clinic over the last 7 months for diarrhea with abdominal pain and/or bloating who were tested for these antibodies. The patients were diagnosed according to the Rome III criteria or with organic disorders, and those presenting with IBS were further classified as post-infectious (PI) or non-PI-IBS...
October 2016: Revista de Gastroenterología de México
Mark Pimentel, Chris Purdy, Raf Magar, Ali Rezaie
PURPOSE: A high incidence of irritable bowel syndrome (IBS) is associated with significant medical costs. Diarrhea-predominant IBS (IBS-D) is diagnosed on the basis of clinical presentation and diagnostic test results and procedures that exclude other conditions. This study was conducted to estimate the potential cost savings of a novel IBS diagnostic blood panel that tests for the presence of antibodies to cytolethal distending toxin B and anti-vinculin associated with IBS-D. METHODS: A cost-minimization (CM) decision tree model was used to compare the costs of a novel IBS diagnostic blood panel pathway versus an exclusionary diagnostic pathway (ie, standard of care)...
July 2016: Clinical Therapeutics
Madhumita Paul, Omar Skalli
Synemin has three splice variants (α, β, and L) with identical head and rod domains but with tail domains of varying size. α- and β-Synemin are larger than most intermediate filament proteins (1565 and 1253 amino acids, respectively) but L-synemin is shorter (339 amino acids). Synemin isoforms do not self-assemble into filaments but can copolymerize with vimentin and desmin. Synemin is present in all muscle cell types, in a few neural cell types, and in various other nonepithelial cell types. Synemin expression is regulated, sometimes in an isoform-specific manner, during development of the nervous system, in brain and breast cancer cells and during injuries to the brain and liver...
2016: Methods in Enzymology
Minako Beppu, Setsu Sawai, Mamoru Satoh, Masahiro Mori, Takahiro Kazami, Sonoko Misawa, Kazumoto Shibuya, Masumi Ishibashi, Kazuyuki Sogawa, Sayaka Kado, Yoshio Kodera, Fumio Nomura, Satoshi Kuwabara
To identify the target molecules of chronic inflammatory demyelinating polyneuropathy (CIDP), we used proteomic-based approach in the extracted proteins from porcine cauda equina. Two of 31 CIDP patients had markedly elevated serum autoantibodies against vinculin, a cell adhesion protein. Both of the patients with anti-vinculin antibodies had similar clinical manifestation, which are compatible with those of "typical" CIDP. Immunocytochemistry showed that vinculin was stained at the myelin sheath of the sciatic nerves by serum samples...
October 15, 2015: Journal of Neuroimmunology
Olaia Colinas, Alejandro Moreno-Domínguez, Hai-Lei Zhu, Emma J Walsh, M Teresa Pérez-García, Michael P Walsh, William C Cole
The myogenic response of resistance arterioles and small arteries involving constriction in response to intraluminal pressure elevation and dilation on pressure reduction is fundamental to local blood flow regulation in the microcirculation. Integrins have garnered considerable attention in the context of initiating the myogenic response, but evidence indicative of mechanotransduction by integrin adhesions, for example established changes in tyrosine phosphorylation of key adhesion proteins, has not been obtained to substantiate this interpretation...
October 1, 2015: Biochemical Pharmacology
Kyojiro Kawakami, Yasunori Fujita, Taku Kato, Kosuke Mizutani, Koji Kameyama, Hiroki Tsumoto, Yuri Miura, Takashi Deguchi, Masafumi Ito
Treatment with taxanes for castration-resistant prostate cancer often leads to the development of resistance. It has been recently demonstrated that exosomes present in the body fluids contain proteins and RNAs in the cells from which they are derived and could serve as a diagnostic marker for various diseases. In the present study, we aimed to identify proteins contained in exosomes that could be markers for progression and taxane-resistance of prostate cancer. Exosomes were isolated by differential centrifugation from the culture medium of taxane-resistant human prostate cancer PC-3 cells (PC-3R) and their parental PC-3 cells...
July 2015: International Journal of Oncology
Mark Pimentel, Walter Morales, Ali Rezaie, Emily Marsh, Anthony Lembo, James Mirocha, Daniel A Leffler, Zachary Marsh, Stacy Weitsman, Kathleen S Chua, Gillian M Barlow, Enoch Bortey, William Forbes, Allen Yu, Christopher Chang
Diarrhea-predominant irritable bowel syndrome (IBS) is diagnosed through clinical criteria after excluding "organic" conditions, and can be precipitated by acute gastroenteritis. Cytolethal distending toxin B (CdtB) is produced by bacteria that cause acute gastroenteritis, and a post-infectious animal model demonstrates that host antibodies to CdtB cross-react with vinculin in the host gut, producing an IBS-like phenotype. Therefore, we assessed circulating anti-CdtB and anti-vinculin antibodies as biomarkers for D-IBS in human subjects...
2015: PloS One
Dominga Iacobazzi, Indira Garaeva, Ambra Albertario, Myriam Cherif, Gianni D Angelini, Massimo Caputo, Mohamed T Ghorbel
BACKGROUND/AIM: Endothelial cell migration is required for physiological angiogenesis, but also contributes to various pathological conditions, including tumour vascularization. The mRNA expression of PP1cβ, the beta isoform of the catalytic PP1 subunit, was shown to be upregulated in chronic hypoxia. Since hypoxia is a major regulator of angiogenesis, the potential role of PP1cβ in angiogenesis was investigated. METHODS: We examined PP1cβ protein level in pediatric heart following chronic hypoxia and found PP1cβ upregulation in cyanotic compared with acyanotic myocardium...
2015: Cellular Physiology and Biochemistry
Jurgen van Heemst, Diahann T S L Jansen, Savvas Polydorides, Antonis K Moustakas, Marieke Bax, Anouk L Feitsma, Diënne G Bontrop-Elferink, Martine Baarse, Diane van der Woude, Gert-Jan Wolbink, Theo Rispens, Frits Koning, René R P de Vries, George K Papadopoulos, Georgios Archontis, Tom W Huizinga, René E Toes
The HLA locus is the strongest risk factor for anti-citrullinated protein antibody (ACPA)(+) rheumatoid arthritis (RA). Despite considerable efforts in the last 35 years, this association is poorly understood. Here we identify (citrullinated) vinculin, present in the joints of ACPA(+) RA patients, as an autoantigen targeted by ACPA and CD4(+) T cells. These T cells recognize an epitope with the core sequence DERAA, which is also found in many microbes and in protective HLA-DRB1*13 molecules, presented by predisposing HLA-DQ molecules...
2015: Nature Communications
Mark Pimentel, Walter Morales, Venkata Pokkunuri, Constantinos Brikos, Sun Moon Kim, Seong Eun Kim, Konstantinos Triantafyllou, Stacy Weitsman, Zachary Marsh, Emily Marsh, Kathleen S Chua, Shanthi Srinivasan, Gillian M Barlow, Christopher Chang
BACKGROUND: Acute gastroenteritis can precipitate irritable bowel syndrome (IBS) in humans. Cytolethal distending toxin is common to all pathogens causing gastroenteritis. Its active subunit, CdtB, is associated with post-infectious bowel changes in a rat model of Campylobacter jejuni infection, including small intestinal bacterial overgrowth (SIBO). AIM: To evaluate the role of host antibodies to CdtB in contributing to post-infectious functional sequelae in this rat model...
May 2015: Digestive Diseases and Sciences
Taneka D Jones, Hamed Naimipour, Shan Sun, Michael Cho, Satish B Alapati
INTRODUCTION: Cell adhesion and migration in bioactive scaffolds require actin cytoskeleton remodeling and focal adhesion formation. Additionally, human dental pulp stem cells (hDPSCs) undergo several changes in their mechanical properties during odontogenic differentiation. The effect of factors essential for odontogenesis on actin stress fiber elasticity and focal adhesion formation is not known. METHODS: Live hDPSCs cultured in odontogenic media were imaged for cytoskeleton changes using an atomic force microscope...
January 2015: Journal of Endodontics
Gideon Nesher
Giant cell arteritis (GCA) is considered to be a T cell-dependent disease. Autoantibodies have not consistently been found in GCA. The exception is antiphospholipid antibodies (APLA), which were found in 30-80% of GCA cases. Recently, efforts have been made to seek autoantibodies in GCA using newer methods of detection: serological identification of antigens by recombinant cDNA expression cloning, and a proteomic approach. In these studies, lamin C (a nuclear envelope antigen) was recognized by antibodies in 32% of GCA sera and none of the controls...
July 2014: Israel Medical Association Journal: IMAJ
Shao-liang Wang, Xiao-hua Shi, Zhi Yang, Yi-ming Zhang, Li-ru Shen, Ze-yuan Lei, Zhi-Qing Zhang, Cong Cao, Dong-li Fan
Medical device implants are drawing increasing amounts of interest from modern medical practitioners. However, this attention is not evenly spread across all such devices; most of these implantable devices can cause adverse reactions such as inflammation, fibrosis, thrombosis, and infection. In this work, the biocompatibility of silicone rubber (SR) was improved through carbon (C) ion implantation. Scanning electron microscopy (SEM), atomic force microscopy (AFM), X-ray photoelectron spectroscopy (XPS), and X-ray diffraction (XRD) results confirmed that these newly generated carbon-implanted silicone rubbers (C-SRs) had large, irregular peaks and deep valleys on their surfaces...
2014: PloS One
Fumihiko Katagiri, Masaharu Takagi, Minako Nakamura, Yoichiro Tanaka, Kentaro Hozumi, Yamato Kikkawa, Motoyoshi Nomizu
Laminins, major components of basement membrane, consist of three different subunits, α, β, and γ chains, and so far, five α, three β, and three γ chains have been identified. We have constructed synthetic peptide libraries derived from the laminin sequences and identified various cell-adhesive peptides. Ten active peptides from the laminin α chain sequences (α1-α5) were found to promote integrin-mediated cell adhesion. Previously, we found fourteen cell-adhesive peptides from the β1 chain sequence but their receptors have not been analyzed...
May 15, 2014: Archives of Biochemistry and Biophysics
Soo Youn Lee, Jung Mi Kim, Soo Young Cho, Hyun Suk Kim, Hee Sun Shin, Jeong Yong Jeon, Rukhsana Kausar, Seon Yong Jeong, Young Seek Lee, Myung Ae Lee
We recently reported that hNSCs (human neural stem cells) have the interesting characteristic of migration towards an intracranial glioma. However, the molecules and mechanisms responsible for tumour tropism are unclear. In the present study, we used microarray and proteomics analyses to identify a novel chemoattractant molecule, TIMP-1 (tissue inhibitor of metalloproteinase-1), secreted from human brain tumour tissues. We demonstrate that TIMP-1 significantly enhances hNSC adhesion and migration in a cell culture system...
May 1, 2014: Biochemical Journal
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