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Wound healing and oxygenation

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https://www.readbyqxmd.com/read/28098885/carboxamide-analog-itr-284-evokes-apoptosis-and-inhibits-migration-ability-in-human-lung-adenocarcinoma-a549-cells
#1
Jai-Sing Yang, Chia-An Lin, Chi-Cheng Lu, Yen-Fang Wen, Fuu-Jen Tsai, Shih-Chang Tsai
Lung adenocarcinoma is the most common type of lung cancer and found in both smokers and non-smokers, but the treatment of lung cancer is limited. ITR-284 has been shown to be a potent carboxamide-derived anticancer agent and to induce apoptosis in leukemia and colon cancer cells. However, little is known whether ITR-284 has anticancer activity in human lung adenocarcinoma cells through induction of apoptosis and suppression of migration in vitro. We showed that ITR-284 inhibited human lung cancer A549 cells using the thiazolyl blue tetrazolium bromide (MTT) assay and evoked apoptosis via the cell cycle distribution at S phase arrest...
January 16, 2017: Oncology Reports
https://www.readbyqxmd.com/read/28078186/soluble-receptor-for-advanced-glycation-end-products-improves-stromal-cell-derived-factor-1-activity-in-model-diabetic-environments
#2
Melissa Przyborowski Olekson, Renea A Faulknor, Henry C Hsia, Ann Marie Schmidt, François Berthiaume
Objective: In diabetes, hyperglycemia causes the accumulation of advanced glycation end products (AGEs) that trigger reactive oxygen species (ROS) generation through binding the receptor for AGEs (RAGE). Because exogenous growth factors have had little success in enhancing chronic wound healing, we investigated whether hyperglycemia-induced AGEs interfere with cellular responses to extracellular signals. We used stromal cell-derived factor-1 (SDF-1), an angiogenic chemokine also known to promote stem cell recruitment in skin wounds...
December 1, 2016: Advances in Wound Care
https://www.readbyqxmd.com/read/28070472/utilizing-the-veraflo%C3%A2-instillation-negative-pressure-wound-therapy-system-with-advanced-care-for-a-case-study
#3
Rita K Driver
BACKGROUND:  Mankind has always suffered wounds throughout time due to trauma, disease, and lifestyles. Many wounds are non-healing and have continued to be challenging. However, utilizing advanced wound care treatments, such as negative pressure wound treatment with instillation and dwell time (NPWTi-d), has proven beneficial. NPWTi-d is indicated in a variety of wounds, such as trauma, surgical, acute, pressure injuries, diabetic foot ulcers, and venous leg ulcers. Bacteria and bioburden interrupts wound healing by increasing the metabolic needs, ingesting, and robbing the necessary nutrients and oxygen...
November 30, 2016: Curēus
https://www.readbyqxmd.com/read/28057304/drosophila-as-a-model-for-human-diseases-focus-on-innate-immunity-in-barrier-epithelia
#4
P Bergman, S Seyedoleslami Esfahani, Y Engström
Epithelial immunity protects the host from harmful microbial invaders but also controls the beneficial microbiota on epithelial surfaces. When this delicate balance between pathogen and symbiont is disturbed, clinical disease often occurs, such as in inflammatory bowel disease, cystic fibrosis, or atopic dermatitis, which all can be in part linked to impairment of barrier epithelia. Many innate immune receptors, signaling pathways, and effector molecules are evolutionarily conserved between human and Drosophila...
2017: Current Topics in Developmental Biology
https://www.readbyqxmd.com/read/28049506/novel-derivative-of-aminobenzenesulfonamide-3c-induces-apoptosis-in-colorectal-cancer-cells-through-ros-generation-and-inhibits-cell-migration
#5
Khayal Al-Khayal, Ahmed Alafeefy, Mansoor-Ali Vaali-Mohammed, Amer Mahmood, Ahmed Zubaidi, Omar Al-Obeed, Zahid Khan, Maha Abdulla, Rehan Ahmad
BACKGROUND: Colorectal cancer (CRC) is the 3(rd) most common type of cancer worldwide. New anti-cancer agents are needed for treating late stage colorectal cancer as most of the deaths occur due to cancer metastasis. A recently developed compound, 3c has shown to have potent antitumor effect; however the mechanism underlying the antitumor effect remains unknown. METHODS: 3c-induced inhibition of proliferation was measured in the absence and presence NAC using MTT in HT-29 and SW620 cells and xCELLigence RTCA DP instrument...
January 3, 2017: BMC Cancer
https://www.readbyqxmd.com/read/28049004/microcirculatory-assessment-of-arterial-below-knee-stumps-near-infrared-spectroscopy-vs-transcutaneous-oxygen-tension-a-preliminary-study-in-prosthesis-users
#6
Davy Laroche, José-Luis Barnay, Bastien Tourlonias, Cyril Orta, Christine Obert, Jean-Marie Casillas
OBJECTIVES: To examine metrological properties of near-infrared spectroscopy (NIRS) vs. transcutaneous oxygen tension (TcPO2) for microcirculatory assessment of vascular transtibial stumps at the stabilized period of prosthesis fitting, as a preliminary step before exploring its ability to predict stump healing, considering the previously identified limits of TcPO2 (borderline area between 15 and 35 mmHg). DESIGN: Prospective single-centre observational study. SETTING: University-based rehabilitation center...
December 31, 2016: Archives of Physical Medicine and Rehabilitation
https://www.readbyqxmd.com/read/28042606/oxygen-sensing-difluoroboron-%C3%AE-diketonate-polylactide-materials-with-tunable-dynamic-ranges-for-wound-imaging
#7
Christopher A DeRosa, Scott A Seaman, Alexander S Mathew, Catherine M Gorick, Ziyi Fan, James N Demas, Shayn M Peirce, Cassandra L Fraser
Difluoroboron β-diketonate poly(lactic acid) materials exhibit both fluorescence (F) and oxygen sensitive room-temperature phosphorescence (RTP). Introduction of halide heavy atoms (Br and I) is an effective strategy to control the oxygen sensitivity in these materials. A series of naphthyl-phenyl (nbm) dye derivatives with hydrogen, bromide and iodide substituents were prepared for comparison. As nanoparticles, the hydrogen derivative was hypersensitive to oxygen (0-0.3%), while the bromide analogue was suited for hypoxia detection (0-3% O2)...
November 23, 2016: ACS Sensors
https://www.readbyqxmd.com/read/28034822/induced-oxidative-stress-management-in-wounds-through-phenolic-acids-engineered-fibrous-protein-an-in-vitro-assessment-using-polymorphonuclear-pmn-cells
#8
T Thiruselvi, S Thirupathi Kumara Raja, S K Shanuja, S Iswarya, A Gnanamani
The present study explores the preparation, characterization and the role of phenolic acid tethered fibrous protein in the management of induced oxidative stress studied under in vitro conditions. In brief, the biomaterial is prepared by engineering the fibrous protein with dihydroxy and trihydroxy phenolic acid moieties and subjected to characterization to ensure the tethering. The resultant biomaterial studied for its efficacy as a free radical scavenger using polymorphonuclear (PMN) cells with induced oxidative stress and also as an agent for cell migration using fibroblasts cells...
December 26, 2016: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28032556/combined-reflectance-and-raman-spectroscopy-to-assess-degree-of-in%C3%A2-vivo-angiogenesis-after-tissue-injury
#9
Shailesh Agarwal, William R Lloyd, Shawn J Loder, Michael T Chung, Charles Hwang, Michael D Morris, Benjamin Levi
BACKGROUND: Angiogenesis, the formation of blood vessels, is a critical aspect of wound healing. Disorders of wound healing are often characterized by lack of angiogenesis, a condition frequently observed in aging and diabetic patients. Current techniques for assessing blood at injury sites are limited to contrast-imaging, including angiography. However, these techniques do not directly observe oxygenation of blood and are not amenable to serial evaluation. A multimodal noninvasive reflectance and Raman spectrometer have been proposed to help clinicians as a point-of-care tool to interrogate local angiogenesis and tissue architecture, respectively...
September 19, 2016: Journal of Surgical Research
https://www.readbyqxmd.com/read/28012599/blood-oxidative-stress-blos-is-a-secondary-host-defense-system-responding-normally-to-anaerobic-wound-infection-and-inadvertently-to-dietary-ultra-exogenous-sulfide-formation-usf
#10
Peter G Stroot
Blood oxidative stress (BLOS) is the presence of white blood cells and platelets that are generating high levels of reactive oxygen species (ROS). A mathematical model links the level of BLOS or BLOS# and plasma sulfide concentration. An increase in the BLOS# reduces the plasma sulfide concentration. The reported maximum plasma sulfide concentration for defined health conditions were used to calculate the minimum BLOS#. Elevated BLOS generates high plasma concentration of ROS, which triggers multiple responses in the body that protect the host...
January 2017: Medical Hypotheses
https://www.readbyqxmd.com/read/28005799/clinical-characteristics-and-treatment-of-osteopetrosis-complicated-by-osteomyelitis-of-the-mandible
#11
Hai-Jiang Sun, Lei Xue, Chuan-Bin Wu, Qing Zhou
Osteopetrosis represents a heterogeneous group of rare, hereditary bone disorders with variable clinical features, and an increase in bone density. Osteomyelitis of the jaws is a significant complication of osteopetrosis. In this article, a reported patient with osteopetrosis complicated by osteomyelitis of the mandible was examined. The patient was treated with intravenous antibiotic therapy, debridement of necrotic bone and hyperbaric oxygen therapy; in addition, the authors attempted to implant the calcium sulfate and vancomycin to reconstruct the bone defect...
November 2016: Journal of Craniofacial Surgery
https://www.readbyqxmd.com/read/27994436/role-of-endogenous-insulin-gene-enhancer-protein-isl-1-in-angiogenesis
#12
Si-Qi Xiong, Hai-Bo Jiang, Yan-Xiu Li, Hai-Bo Li, Hui-Zhuo Xu, Zhen-Kai Wu, Wei Zheng, Xiao-Bo Xia
OBJECTIVE: To elucidate the role of insulin gene enhancer protein ISL-1 (Islet-1) in angiogenesis and regulation of vascular endothelial growth factor (VEGF) expression in vitro and in vivo. METHODS: siRNA targeting Islet-1 was transfected to human umbilical vein endothelial cell lines (HUVECs). The expression of Islet-1 and VEGF in the cultured cells was measured using real-time PCR and immunoblotting. 3-[4,5-dimethylthiazol-2-yl]-2,5- diphenyltetrazolium bromide; thiazolyl blue (MTT) assay was used to analyze the proliferation of HUVECs affected by Islet-1...
2016: Molecular Vision
https://www.readbyqxmd.com/read/27992567/impact-of-hyperglycemia-and-low-oxygen-tension-on-adipose-derived-stem-cells-compared-with-dermal-fibroblasts-and-keratinocytes-importance-for-wound-healing-in-type-2-diabetes
#13
Aurore Lafosse, Cécile Dufeys, Christophe Beauloye, Sandrine Horman, Denis Dufrane
AIM: Adipose-derived stem cells (ASC) are currently proposed for wound healing in those with type 2 diabetes mellitus (T2DM). Therefore, this study investigated the impact of diabetes on adipose tissue in relation to ASC isolation, proliferation, and growth factor release and the impact of hyperglycemia and low oxygen tension (found in diabetic wounds) on dermal fibroblasts, keratinocytes, and ASC in vitro. METHODS: Different sequences of hypoxia and hyperglycemia were applied in vitro to ASC from nondiabetic (n = 8) or T2DM patients (n = 4) to study cell survival, proliferation, and growth factor release...
2016: PloS One
https://www.readbyqxmd.com/read/27990309/a-case-of-periodontal-necrosis-following-embolization-of-maxillary-artery-for-epistaxis
#14
Kohei Nishimoto, Ryosei Minoda, Ryoji Yoshida, Toshinori Hirai, Eiji Yumoto
Embolization of the maxillary artery (MA) is a common treatment modality for refractory epistaxis. Tissue necrosis after embolization of the MA is a rare complication. Here, we reported the first case of the development of necrosis of soft tissue and alveolar bone in the periodontium after embolization. A 48-year-old man with poor oral hygiene and a heavy smoking habit was referred to our clinic due to intractable epistaxis. After treatment with anterior-posterior nasal packing (AP nasal packing), the epistaxis relapsed...
2016: Case Reports in Otolaryngology
https://www.readbyqxmd.com/read/27988353/the-effects-of-urotensin-ii-on-migration-and-invasion-are-mediated-by-nadph-oxidase-derived-reactive-oxygen-species-through-the-c-jun-n-terminal-kinase-pathway-in-human-hepatoma-cells
#15
Ying-Ying Li, Zheng-Ming Shi, Xiao-Tong Yu, Ping Feng, Xue-Jiang Wang
AIMS: Urotensin II (UII) is a vasoactive neuropeptide involved in migration and invasion in various cell types. However, the effects of UII on human hepatoma cells still remain unclear. The aim of this study was to investigate the role and mechanism of UII on migration and invasion in human hepatoma cells. METHODS: Migration was measured by wound healing assays and a Transwell(®) methodology, and invasion was analyzed using Matrigel(®) invasion chambers. Reactive oxygen species (ROS) levels were detected using a 2', 7'-dichlorofluorescein diacetate probe, and flow cytometry, and protein expression levels were evaluated by western blotting...
December 14, 2016: Peptides
https://www.readbyqxmd.com/read/27965040/hemodynamic-shear-stress-stimulates-migration-and-extravasation-of-tumor-cells-by-elevating-cellular-oxidative-level
#16
Shijun Ma, Afu Fu, Geraldine Giap Ying Chiew, Kathy Qian Luo
Cancer cells are shed into the blood stream and are exposed to hemodynamic shear stress during metastasis. It has been shown that shear stress can destroy circulating tumor cells (CTCs) both in vitro and in vivo. However, it remains unclear whether shear stress can modulate the properties and functions of tumor cells in a manner that might help CTCs to exit circulation. In this study, we established a microfluidic circulatory system to apply physiological fluid shear stress on breast cancer cells and demonstrated that an arterial level of shear stress significantly enhanced tumor cell migration in transwell and wound healing assays, and enhanced extravasation in a transendothelial assay...
December 10, 2016: Cancer Letters
https://www.readbyqxmd.com/read/27959618/mechanisms-of-organ-injury-and-repair-by-macrophages
#17
Kevin M Vannella, Thomas A Wynn
Macrophages regulate tissue regeneration following injury. They can worsen tissue injury by producing reactive oxygen species and other toxic mediators that disrupt cell metabolism, induce apoptosis, and exacerbate ischemic injury. However, they also produce a variety of growth factors, such as IGF-1, VEGF-α, TGF-β, and Wnt proteins that regulate epithelial and endothelial cell proliferation, myofibroblast activation, stem and tissue progenitor cell differentiation, and angiogenesis. Proresolving macrophages in turn restore tissue homeostasis by functioning as anti-inflammatory cells, and macrophage-derived matrix metalloproteinases regulate fibrin and collagen turnover...
December 7, 2016: Annual Review of Physiology
https://www.readbyqxmd.com/read/27959452/vitamin-k4-inhibits-the-proliferation-and-induces-apoptosis-of-u2os-osteosarcoma-cells-via-mitochondrial-dysfunction
#18
Weihua Di, Muhammad Khan, Yong Gao, Jing Cui, Deqiang Wang, Mingfen Qu, Liangtao Feng, Amara Maryam, Hongwen Gao
Vitamin K (VK) is a group of fat‑soluble vitamins, which serve important roles in blood coagulation and bone metabolism. A recent study reported that several VK subtypes possess antitumor properties, however the antitumor effects of VK in osteosarcoma are unknown. The present study aimed to identify the antitumor effects of VK in osteosarcoma and the possible underlying mechanism of action. The effect of VK4 on cell viability was determined using a 3‑(4,5‑dimethylthiazol‑2‑yl)‑2,5‑diphenyltetrazolium bromide (MTT) assay...
January 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/27938770/successful-healing-of-tracheal-radionecrosis-role-of-hyperbaric-oxygen-therapy
#19
Miguel Ariza-Prota, Arturo Morales, Jose Grajeda, Rosa López-Lisbona, Noelia Cubero, Jordi Dorca, Antoni Rosell
Hyperbaric oxygen therapy, the administration of 100% oxygen at pressures > 1 atm, is believed to promote wound healing by increasing angiogenesis and collagen synthesis. To our knowledge, this treatment modality has never been described in patients with tracheal radionecrosis. Here, we report the case of a 55-year-old man diagnosed with stage IIIB lung adenocarcinoma who was treated with chemotherapy and concomitant external intensity-modulated radiotherapy involving the left lung and mediastinum. Nine months later, he presented with neck pain, cough with mucopurulent sputum, and fever...
December 2016: Chest
https://www.readbyqxmd.com/read/27909641/a-case-series-of-complex-recalcitrant-wounds-treated-with-epidermal-grafts-harvested-from-an-automated-device
#20
Stephen S Cai, Arvind U Gowda, Karan Chopra, Rachel Waldman, Ronald P Silverman, Yvonne M Rasko
INTRODUCTION: Epidermal grafting has several advantages over full-thickness or split-thickness grafts in the treatment of complex non-healing wounds. These include the low risk of donor site complications, minimal patient discomfort, and abstention from the operating room. Traditionally, the lack of reliable epidermal harvesting techniques has limited its clinical utilization. The development of an automated suction blister epidermal graft (SBEG) harvesting device may facilitate clinical utilization of this technique...
October 30, 2016: Curēus
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