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Invadopodia

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https://www.readbyqxmd.com/read/27921151/free-boundary-problem-for-cell-protrusion-formations-theoretical-and-numerical-aspects
#1
Olivier Gallinato, Masahito Ohta, Clair Poignard, Takashi Suzuki
In this paper, a free boundary problem for cell protrusion formation is studied theoretically and numerically. The cell membrane is precisely described thanks to a level set function, whose motion is due to specific signalling pathways. The aim is to model the chemical interactions between the cell and its environment, in the process of invadopodia or pseudopodia formation. The model consists of Laplace equation with Dirichlet condition inside the cell coupled to Laplace equation with Neumann condition in the outer domain...
December 5, 2016: Journal of Mathematical Biology
https://www.readbyqxmd.com/read/27912101/the-cytoskeleton-architecture-of-algivorous-protoplast-feeders-viridiraptoridae-rhizaria-indicates-actin-guided-perforation-of-prey-cell-walls
#2
Anna Busch, Sebastian Hess
Several distantly related, phagotrophic microeukaryotes share the remarkable ability to perforate foreign cell walls in a well-defined pattern to acquire protoplast material as food. The underlying cellular processes, especially the local application of cell wall degrading agents, are still unexplored. We examined the distribution of F-actin and alpha-tubulin in the algivorous, viridiraptorid amoeboflagellates Orciraptor agilis and Viridiraptor invadens over their life histories using phalloidin conjugates and immunolocalization...
October 31, 2016: Protist
https://www.readbyqxmd.com/read/27909076/rab40b-and-tks5-interact-at-invadopodia-during-metastasis
#3
(no author information available yet)
No abstract text is available yet for this article.
December 1, 2016: Journal of Cell Science
https://www.readbyqxmd.com/read/27901093/macrophage-dependent-tumor-cell-transendothelial-migration-is-mediated-by-notch1-mena-inv-initiated-invadopodium-formation
#4
Jeanine Pignatelli, Jose Javier Bravo-Cordero, Minna Roh-Johnson, Saumil J Gandhi, Yarong Wang, Xiaoming Chen, Robert J Eddy, Alice Xue, Robert H Singer, Louis Hodgson, Maja H Oktay, John S Condeelis
The process of intravasation involving transendothelial migration is a key step in metastatic spread. How the triple cell complex composed of a macrophage, Mena over-expressing tumor cell and endothelial cell, called the tumor microenvironment of metastasis (TMEM), facilitates tumor cell transendothelial migration is not completely understood. Previous work has shown that the physical contact between a macrophage and tumor cell results in the formation of invadopodia, actin-rich matrix degrading protrusions, important for tumor cell invasion and transendothelial migration and tumor cell dissemination...
November 30, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27888616/ccl2-egf-positive-feedback-loop-between-cancer-cells-and-macrophages-promotes-cell-migration-and-invasion-in-head-and-neck-squamous-cell-carcinoma
#5
Lu Gao, Feng-Qin Wang, Hui-Min Li, Jie-Gang Yang, Jian-Gang Ren, Ke-Fei He, Bing Liu, Wei Zhang, Yi-Fang Zhao
Head and neck squamous cell carcinoma (HNSCC) represents the most frequent malignancy in the head and neck region, and the survival rate has not been improved significantly over the past three decades. It has been reported the infiltrated macrophages contribute to the malignant progression of HNSCC. However, the crosstalk between macrophages and cancer cells remains poorly understood. In the present study, we explored interactions between monocytes/macrophages and HNSCC cells by establishing the direct co-culture system, and found that the crosstalk promoted the migration and invasion of cancer cells by enhancing the invadopodia formation through a CCL2/EGF positive feedback loop...
November 23, 2016: Oncotarget
https://www.readbyqxmd.com/read/27845909/platelet-derived-growth-factor-receptor-alpha-mediates-nodal-metastases-in-papillary-thyroid-cancer-by-driving-the-epithelial-mesenchymal-transition
#6
Esther Ekpe-Adewuyi, Ana Lopez-Campistrous, Xiaoyun Tang, David N Brindley, Todd P W McMullen
Recently platelet derived growth factor receptor-alpha (PDGFRα) was recognized as a potential target to treat aggressive papillary thyroid cancer given its strong association with lymph node metastases. However, it is unclear how PDGFRα potentiates metastases and if it works through the canonical MAPK pathway traditionally linked to PTC oncogenesis. We explored the phenotypic changes driven by PDGFRα activation in human papillary thyroid cancer (PTC) cells and the downstream signalling cascades through which they are effected...
November 11, 2016: Oncotarget
https://www.readbyqxmd.com/read/27824079/mena-inv-dysregulates-cortactin-phosphorylation-to-promote-invadopodium-maturation
#7
Maxwell D Weidmann, Chinmay R Surve, Robert J Eddy, Xiaoming Chen, Frank B Gertler, Ved P Sharma, John S Condeelis
Invadopodia, actin-based protrusions of invasive carcinoma cells that focally activate extracellular matrix-degrading proteases, are essential for the migration and intravasation of tumor cells during dissemination from the primary tumor. We have previously shown that cortactin phosphorylation at tyrosine residues, in particular tyrosine 421, promotes actin polymerization at newly-forming invadopodia, promoting their maturation to matrix-degrading structures. However, the mechanism by which cells regulate the cortactin tyrosine phosphorylation-dephosphorylation cycle at invadopodia is unknown...
November 8, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27802184/the-role-of-tks-adaptor-proteins-in-invadopodia-formation-growth-and-metastasis-of-melanoma
#8
Shinji Iizuka, Christopher Abdullah, Matthew D Buschman, Begoña Diaz, Sara A Courtneidge
Metastatic cancer cells are characterized by their ability to degrade and invade through extracellular matrix. We previously showed that the Tks adaptor proteins, Tks4 and Tks5, are required for invadopodia formation and/or function in Src-transformed fibroblasts and a number of human cancer cell types. In this study, we investigated the role of Tks adaptor proteins in melanoma cell invasion and metastasis. Knockdown of either Tks4 or Tks5 in both mouse and human melanoma cell lines resulted in a decreased ability to form invadopodia and degrade extracellular matrix...
October 27, 2016: Oncotarget
https://www.readbyqxmd.com/read/27798239/phosphatidylinositol-4-kinase-ii%C3%AE-negatively-regulates-invadopodia-formation-and-suppresses-an-invasive-cellular-phenotype
#9
Ganiyu Olabanji Alli-Balogun, Christina A Gewinner, Ruth Jacobs, Janos Kriston-Vizi, Mark G Waugh, Shane Minogue
The type II PI 4-kinases enzymes synthesise the lipid phosphatidylinositol 4-phosphate (PI(4)P) which has been detected at the Golgi complex and endosomal compartments, and which recruits clathrin adaptors. Despite common mechanistic similarities between the isoforms, the extent of their redundancy is unclear.We found that depletion of PI4KIIα and PI4KIIβ using siRNA led to actin remodelling. Depletion of PI4KIIβ also induced the formation of invadopodia containing membrane type I matrix metalloproteinase (MT1-MMP)...
October 26, 2016: Molecular Biology of the Cell
https://www.readbyqxmd.com/read/27789576/the-role-and-regulation-of-rab40b-tks5-complex-during-invadopodia-formation-and-cancer-cell-invasion
#10
Abitha Jacob, Erik Linklater, Brian A Bayless, Traci Lyons, Rytis Prekeris
Invadopodia formation and extracellular matrix degradation are key events during cancer cell invasion, yet little is known about mechanisms mediating these processes. Here, we report that Rab40b plays a key role in mediating invadopodia function during breast cancer cell invasion. We also identify Tks5 (also known as SH3PXD2A), a known Src kinase substrate, as a new Rab40b effector protein and show that Tks5 functions as a tether that mediates Rab40b-dependent targeting of transport vesicles containing MMP2 and MMP9 to the extending invadopodia...
December 1, 2016: Journal of Cell Science
https://www.readbyqxmd.com/read/27780861/p70s6k1-s6k1-mediated-phosphorylation-regulates-phosphatidylinositol-4-phosphate-5-kinase-type-i-%C3%AE-degradation-and-cell-invasion
#11
Naser Jafari, Qiaodan Zheng, Liqing Li, Wei Li, Lei Qi, Jianyong Xiao, Tianyan Gao, Cai Huang
Phosphatidylinositol 4-phosphate 5-kinase type I γ (PIPKIγ90) ubiquitination and subsequent degradation regulate focal adhesion assembly, cell migration, and invasion. However, it is unknown how upstream signals control PIPKIγ90 ubiquitination or degradation. Here we show that p70S6K1 (S6K1), a downstream target of mechanistic target of rapamycin (mTOR), phosphorylates PIPKIγ90 at Thr-553 and Ser-555 and that S6K1-mediated PIPKIγ90 phosphorylation is essential for cell migration and invasion. Moreover, PIPKIγ90 phosphorylation is required for the development of focal adhesions and invadopodia, key machineries for cell migration and invasion...
December 2, 2016: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/27776431/safflower-yellow-prevents-pulmonary-metastasis-of-breast-cancer-by-inhibiting-tumor-cell-invadopodia
#12
Huiying Fu, Renjie Wu, Yuanyuan Li, Lizong Zhang, Xiaofang Tang, Jue Tu, Weimin Zhou, Jianchao Wang, Qiyang Shou
Carthamus tinctorius L. is a traditional Chinese medicine that activates blood circulation and dissipates blood stasis, and has been extensively used as antitumor treatment in a clinical setting in single or in compound preparation form. However, empirical evidence and a better understanding of the possible mechanisms involved are still required. Here, we investigated the role of safflower yellow (SY), the active ingredient of C. tinctorius, in the pulmonary metastasis of breast cancer, and the underlying mechanism of action...
October 25, 2016: American Journal of Chinese Medicine
https://www.readbyqxmd.com/read/27771248/dynamin2-gtpase-contributes-to-invadopodia-formation-in-invasive-bladder-cancer-cells
#13
Yubai Zhang, Maya Nolan, Hiroshi Yamada, Masami Watanabe, Yasutomo Nasu, Kohji Takei, Tetsuya Takeda
Cancer cell invasion is mediated by actin-based membrane protrusions termed invadopodia. Invadopodia consist of "core" F-actin bundles associated with adhesive and proteolytic machineries promoting cell invasion by degrading extracellular matrix (ECM). Formation of the F-actin core in invadopodia is regulated by various actin-binding proteins including Arp2/3 complex and cortactin. Dynamin GTPase localizes to the invadopodia and is implicated in cancer cell invasion, but its precise role at the invadopodia remained elusive...
October 19, 2016: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/27770278/comprehensive-proteome-profiling-of-glioblastoma-derived-extracellular-vesicles-identifies-markers-for-more-aggressive-disease
#14
Duthika M Mallawaaratchy, Susannah Hallal, Ben Russell, Linda Ly, Saeideh Ebrahimkhani, Heng Wei, Richard I Christopherson, Michael E Buckland, Kimberley L Kaufman
Extracellular vesicles (EVs) play key roles in glioblastoma (GBM) biology and represent novel sources of biomarkers that are detectable in the peripheral circulation. Despite this notionally non-invasive approach to assess GBM tumours in situ, a comprehensive GBM EV protein signature has not been described. Here, EVs secreted by six GBM cell lines were isolated and analysed by quantitative high-resolution mass spectrometry. Overall, 844 proteins were identified in the GBM EV proteome, of which 145 proteins were common to EVs secreted by all cell lines examined; included in the curated EV compendium (Vesiclepedia_559; http://microvesicles...
October 21, 2016: Journal of Neuro-oncology
https://www.readbyqxmd.com/read/27765920/pak4-suppresses-pdz-rhogef-activity-to-drive-invadopodia-maturation-in-melanoma-cells
#15
Nicole S Nicholas, Aikaterini Pipili, Michaela S Lesjak, Simon M Ameer-Beg, Jenny L C Geh, Ciaran Healy, Alistair D MacKenzie Ross, Maddy Parsons, Frank O Nestle, Katie E Lacy, Claire M Wells
Cancer cells are thought to use actin rich invadopodia to facilitate matrix degradation. Formation and maturation of invadopodia requires the co-ordained activity of Rho-GTPases, however the molecular mechanisms that underlie the invadopodia lifecycle are not fully elucidated. Previous work has suggested a formation and disassembly role for Rho family effector p-21 activated kinase 1 (PAK1) however, related family member PAK4 has not been explored. Systematic analysis of isoform specific depletion using in vitro and in vivo invasion assays revealed there are differential invadopodia-associated functions...
September 27, 2016: Oncotarget
https://www.readbyqxmd.com/read/27694340/talin2-mediated-traction-force-drives-matrix-degradation-and-cell-invasion
#16
Lei Qi, Naser Jafari, Xiang Li, Zaozao Chen, Liqing Li, Vesa P Hytönen, Benjamin T Goult, Chang-Guo Zhan, Cai Huang
Talin binds to β-integrin tails to activate integrins, regulating cell migration, invasion and metastasis. There are two talin genes, TLN1 and TLN2, encoding talin1 and talin2, respectively. Talin1 regulates focal adhesion dynamics, cell migration and invasion, whereas the biological function of talin2 is not clear and, indeed, talin2 has been presumed to function redundantly with talin1. Here, we show that talin2 has a much stronger binding to β-integrin tails than talin1. Replacement of talin2 Ser339 with Cys significantly decreased its binding to β1-integrin tails to a level comparable to that of talin1...
October 1, 2016: Journal of Cell Science
https://www.readbyqxmd.com/read/27693494/bradykinin-promotes-migration-and-invasion-of-hepatocellular-carcinoma-cells-through-trpm7-and-mmp2
#17
Yijie Chen, Yang Yu, Shuya Sun, Zhaotong Wang, Peiqing Liu, Shenglan Liu, Jianmin Jiang
Tumor metastasis is the main reason of death for hepatocellular carcinoma (HCC) patients. Cell migration and invasion are two prerequisites for tumor metastasis, in which TRPM7 and MMPs play an important role. In our study, we found that bradykinin (BK) could upregulate the expression of TRPM7 and dynamically regulate the phosphorylation of non-muscle myosin IIA heavy chain (NMHC-IIA) Ser-1943 in HepG2 cells. The influx of Ca(2+) via TRPM7 was necessary for elevating the activity of m-calpain and μ-calpain...
September 30, 2016: Experimental Cell Research
https://www.readbyqxmd.com/read/27690729/endothelin-1-receptor-drives-invadopodia-exploiting-how-%C3%AE-arrestin-1-guides-the-way
#18
Anna Bagnato, Laura Rosanò
Metastatization is a complex multistep process requiring fine-tuned regulated cytoskeleton re-modeling, mediated by the cross-talk of actin with interacting partners, such as the Rho GTPases. Our expanding knowledge of invadopodia, small invasive membrane protrusions composed of a core of F-actin, actin regulators and actin-binding proteins, and hotspots for secretion of extracellular matrix (ECM) proteinases, contributes to clarify critical steps of the metastatic program. Growth factor receptors and their intermediate signaling molecules, along with matrix adhesion and rigidity, pH and hypoxia, act as drivers of cytoskeleton changes and invadopodia formation...
October 3, 2016: Small GTPases
https://www.readbyqxmd.com/read/27626172/nestin-suppression-attenuates-invasive-potential-of-endometrial-cancer-cells-by-downregulating-tgf-%C3%AE-signaling-pathway
#19
Amber A Bokhari, Tabari M Baker, Batsukh Dorjbal, Sana Waheed, Christopher M Zahn, Chad A Hamilton, G Larry Maxwell, Viqar Syed
Nestin, an intermediate filament protein and a stem cell marker is expressed in several tumors. Until recently, little was known about the expression levels and the role of Nestin in endometrial cancer. Compared to the immortalized endometrial epithelial cell line EM-E6/E7-TERT, endometrial cancer cell lines express high to moderate levels of Nestin. Furthermore, endometrial tumors and tumor cell lines have a cancer stem-like cell subpopulation expressing CD133. Among the cancer lines, AN3CA and KLE cells exhibited both a significantly higher number of CD133+ cells and expressed Nestin at higher levels than Ishikawa cells...
September 10, 2016: Oncotarget
https://www.readbyqxmd.com/read/27597754/5-inositol-phosphatase-ship2-recruits-mena-to-stabilize-invadopodia-for-cancer-cell-invasion
#20
Charles V Rajadurai, Serhiy Havrylov, Paula P Coelho, Colin D H Ratcliffe, Kossay Zaoui, Bruce H Huang, Anie Monast, Naila Chughtai, Veena Sangwan, Frank B Gertler, Peter M Siegel, Morag Park
Invadopodia are specialized membrane protrusions that support degradation of extracellular matrix (ECM) by cancer cells, allowing invasion and metastatic spread. Although early stages of invadopodia assembly have been elucidated, little is known about maturation of invadopodia into structures competent for ECM proteolysis. The localized conversion of phosphatidylinositol(3,4,5)-triphosphate and accumulation of phosphatidylinositol(3,4)-bisphosphate at invadopodia is a key determinant for invadopodia maturation...
September 12, 2016: Journal of Cell Biology
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