Jovan S Brockett, Tad Manalo, Hala Zein-Sabatto, Jina Lee, Junnan Fang, Philip Chu, Harry Feng, Dattatraya Patil, Priscilla Davidson, Kenneth Ogan, Viraj A Master, John G Pattaras, David L Roberts, Sharon H Bergquist, Matthew A Reyna, John A Petros, Dorothy A Lerit, Rebecca S Arnold
Mutations in SETD2 are among the most prevalent drivers of renal cell carcinoma (RCC). We identified a novel single nucleotide polymorphism (SNP) in SETD2, E902Q, within a subset of RCC patients, which manifests as both an inherited or tumor-associated somatic mutation. To determine if the SNP is biologically functional, we used CRISPR-based genome editing to generate the orthologous mutation within the Drosophila melanogaster Set2 gene. In Drosophila, the homologous amino acid substitution, E741Q, reduces H3K36me3 levels comparable to Set2 knockdown, and this loss is rescued by reintroduction of a wild-type Set2 transgene...
January 30, 2024: Genetics