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https://www.readbyqxmd.com/read/28454458/survivin-downregulation-using-sirna-nanoliposomes-inhibits-cell-proliferation-and-promotes-the-apoptosis-of-mhcc-97h-hepatic-cancer-cells-an-in-vitro-and-in-vivo-study
#1
Ziqin Liu, Tianyou Wang, Zhaoxia Zhang, Suoqin Tang, Shunqiao Feng, Mei Yue, Mengze Hu, Litian Xuan, Yanfei Chen
At present, survivin is one of the most cancer-specific proteins that has been identified. The present study aimed to investigate the antitumor effects of novel survivin small interfering RNA (siRNA) nanoliposomes targeting survivin in human hepatocellular carcinoma MHCC-97H cells and xenograft mouse models. Survivin-targeted siRNA nanoliposomes were prepared and transfected into MHCC-97H cells and MHCC-97H-bearing nude mice. Survivin expression was analyzed using reverse transcription-quantitative polymerase chain reaction (RT-qPCR) and western blotting...
April 2017: Oncology Letters
https://www.readbyqxmd.com/read/28454443/mir-185-3p-regulates-the-invasion-and-metastasis-of-nasopharyngeal-carcinoma-by-targeting-wnt2b-in-vitro
#2
Chao Liu, Guo Li, Shuling Ren, Zhongwu Su, Yunyun Wang, Yongquan Tian, Yong Liu, Yuanzheng Qiu
MicroRNAs (miRs) have been recognised as important regulators of malignant behaviour in different types of human cancer, including nasopharyngeal carcinoma (NPC). A previous study by our group revealed that miR-185-3p regulates the radioresistance of NPC cells. The present study aimed to investigate the effect of miR-185-3p on NPC invasion and metastasis. Human NPC CNE-2 and 5-8F cell lines were transfected with a miR-185-3p mimic and miR-185-3p inhibitor, respectively, and their effects on the invasion and metastasis of these cells was assessed using a wound healing assay and Matrigel invasion assay...
April 2017: Oncology Letters
https://www.readbyqxmd.com/read/28454422/the-role-of-mrp1-in-the-multidrug-resistance-of-colorectal-cancer
#3
Dongxing Cao, Shaolan Qin, Yifei Mu, Ming Zhong
The role of multidrug resistance associated protein 1 (MRP1) in the multidrug resistance (MDR) of colorectal cancer (CRC) remains unclear. The present study aimed to investigate the effect of MRP1 in MDR CRC and its therapeutic potential for the treatment of patients with this disease. The human MDR CRC cell lines HCT-8 and Colo205 were established through stable exposure to 5-florouracil (5-FU) over a 5-month period. MRP1 was knocked-down in MDR CRC cells through the transfection of short hairpin RNA targeting MRP1 (shMRP1)...
April 2017: Oncology Letters
https://www.readbyqxmd.com/read/28454402/mir-215-suppresses-proliferation-and-migration-of-non-small-cell-lung-cancer-cells
#4
Xiaopan Cai, Dongyu Peng, Haifeng Wei, Xinghai Yang, Quan Huang, Zaijun Lin, Wei Xu, Ming Qian, Cheng Yang, Tielong Liu, Wangjun Yan, Jian Zhao
The expression of microRNA-215 (miR-215) in non-small cell lung cancer (NSCLC) tissues and the effects of miR-215 on the proliferation and migration of NSCLC cells were investigated. qRT-PCR was used to detect the expression of miR-215 in NSCLC tissues and paired normal tumor-adjacent lung tissues; MTT assay, transwell assay and soft-agar assay were used in vitro to evaluate the role of miR-215 on proliferation, migration and cell clonality on NSCLC cells, after transfecting miR-215 mimics to NSCLC cell line A549 or miR-215 to H1299...
April 2017: Oncology Letters
https://www.readbyqxmd.com/read/28454397/sphingosine-kinase-1-a-novel-independent-prognosis-biomarker-in-hepatocellular-carcinoma
#5
Huajie Cai, Xuemeng Xie, Ling Ji, Xiaojiao Ruan, Zhihai Zheng
Sphingosine kinase 1 (Sphk1) is an oncogenic kinase that is responsible for the phosphorylation of sphingosine to sphingosine-1-phosphate (S1P). Mounting evidence suggests that Sphk1 serves a crucial role in the proliferation and development of a variety of human cancer cells. However, the role of Sphk1 in hepatocellular carcinoma (HCC) has not been fully elucidated. Therefore, the expression of Sphk1 was examined in 127 formalin-fixed, paraffin-embedded HCC tissues using immunohistochemistry, and its clinical implications and prognostic significance were analyzed...
April 2017: Oncology Letters
https://www.readbyqxmd.com/read/28454381/optimized-construction-of-muc1-vntrn-dna-vaccine-and-its-anti-pancreatic-cancer-efficacy
#6
Yuan-Feng Gong, Quan-Bo Zhou, Ya-Di Liao, Cong Mai, Tie-Jun Chen, Yun-Qiang Tang, Ru-Fu Chen
Considering mucin 1-variable number tandem repeat (MUC1-VNTRn) as a novel target for pancreatic cancer immunotherapy, the present study aimed to screen and identify the pVAX1-MUC1-VNTRn DNA vaccine with the strongest immunogenicity. Following construction of a pVAX1-MUC1-VNTRn plasmid, immature dendritic cells (DCs) were subjected to transfection, and mature DCs were then co-cultured with autologous T-cells. The numbers of cytotoxic T lymphocytes (CTLs) secreting interferon (IFN)-γ were determined using an enzyme-linked immunospot assay, and CytoTox(®) was also used to examine the MUC1-VNTRn-specific Lethal effect of CTLs on Capan2 cells...
April 2017: Oncology Letters
https://www.readbyqxmd.com/read/28454350/mir-455-5p-functions-as-a-potential-oncogene-by-targeting-galectin-9-in-colon-cancer
#7
Qianqian Yang, Chen Hou, Da Huang, Chunbo Zhuang, Weichao Jiang, Zhi Geng, Xiaobei Wang, Lihua Hu
Although there is evidence that galectin-9 is a critical factor in health and disease, the upstream regulatory microRNA (miRNA or miR) of the protein remains poorly defined. miR-455-5p is characterized as a tumor-associated miRNA in cancer research. However, the actual role of miR-455-5p with respect to inhibiting or promoting tumorigenesis in colon cancer is unclear. The present study aimed to investigate the expression, role and target regulation association of galectin-9 and miR-455-5p in colon cancer. Western blot analysis and reverse transcription-quantitative polymerase chain reaction were used for the detection of the expression levels of galectin-9 and miRNAs...
March 2017: Oncology Letters
https://www.readbyqxmd.com/read/28454346/microrna-134-targets-kras-to-suppress-breast-cancer-cell-proliferation-migration-and-invasion
#8
Xiaomei Su, Ling Zhang, Hua Li, Peng Cheng, Yajie Zhu, Zhen Liu, Yu Zhao, Hongyu Xu, Dong Li, Hui Gao, Tao Zhang
The expression patterns and functions of microRNA-134 (miR-134) have been previously studied in numerous types of cancer. To the best of our knowledge, this is the first study of miR-134 in human breast cancer. In the present study, the expression patterns, biological functions and underlying molecular mechanisms of miR-134 in human breast cancer were investigated. Reverse transcription-quantitative polymerase chain reaction evaluated the expression of miR-134 in human breast cancer tissues, matched normal adjacent tissues, breast cancer cell lines and a normal mammary epithelial cell line...
March 2017: Oncology Letters
https://www.readbyqxmd.com/read/28454344/galectin-7-promotes-the-invasiveness-of-human-oral-squamous-cell-carcinoma-cells-via-activation-of-erk-and-jnk-signaling
#9
Jia-Ping Guo, Xiao-Guang Li
Galectin-7 is a member of the β-galactoside-binding protein family, and is highly expressed in oral squamous cell carcinoma (OSCC). The aim of the present study was to investigate the effects of manipulating galectin-7 expression on the biological phenotype of human OSCC cells and the associated molecular mechanisms. Knockdown of endogenous galectin-7 via small interfering RNA (siRNA) was performed and cell proliferation, apoptosis, migration, and invasion were subsequently assessed. The data indicated that galectin-7 silencing had no impact on the proliferation or apoptosis of OSCC cells...
March 2017: Oncology Letters
https://www.readbyqxmd.com/read/28454339/puma-decreases-the-growth-of-prostate-cancer-pc-3-cells-independent-of-p53
#10
Zhengfei Shan, Qingzuo Liu, Yuling Li, Jitao Wu, Dekang Sun, Zhenli Gao
PUMA (p53 upregulated modulator of apoptosis), a member of the B-cell lymphoma 2 (Bcl-2) protein family, is a pro-apoptotic protein. PUMA expression is modulated by the tumor suppressor p53. PUMA has a role in rapid cell death via p53-dependent and -independent mechanisms. To evaluate whether p53 is required for PUMA-mediated apoptosis in prostate cancer cells, p53 protein was silenced in human prostate cancer PC-3 cells by using p53 small interfering RNA (siRNA). The interference efficiency of p53 on RNA and protein levels was detected by reverse transcription-quantitative polymerase chain reaction and western blotting...
March 2017: Oncology Letters
https://www.readbyqxmd.com/read/28454323/microrna-29a-b-c-targets-inos-and-is-involved-in-protective-remote-ischemic-preconditioning-in-an-ischemia-reperfusion-rat-model-of-non-alcoholic-fatty-liver-disease
#11
Yun-Fei Duan, Dong-Lin Sun, Jing Chen, Feng Zhu, Yong An
Remote ischemic preconditioning (RIPC) protects against the injury that is incurred by ischemia and reperfusion (IR); however, the role of RIPC in liver IR injury in non-alcoholic fatty liver disease (NAFLD) remains unclear. In this study, a NAFLD rat model was utilized in a series of different surgical procedures and molecular experiments. Rats of the IR group and the RIPC+IR group exhibited more severe injury than NAFLD control rats (in which the liver was prodded following a median-incision laparotomy). The liver condition, measured by serum alanine transaminase and aspartate transaminase levels, of the RIPC+IR group was better than that of the IR group...
March 2017: Oncology Letters
https://www.readbyqxmd.com/read/28454319/construction-and-expression-of-a-lentivirus-expression-vector-carrying-the-vegf165-egfp-fusion-gene-in-breast-cancer-mcf-7-cells
#12
Minna Luo, Huan Huang, Lei Hou, Shan Shao, Shangke Huang, Xinhan Zhao
Vascular endothelial growth factor (VEGF)165 is one of the most abundant and potent angiogenic factors in both physiological and pathological conditions. However, the function and mechanism of VEGF165 in tumors and their environment remain to be elucidated. In the present study, a lentivirus vector (LV) that contained the VEGF165-enhanced green fluorescent protein (EGFP) fusion gene was constructed and transfected into the human breast cancer cell line MCF-7. Following transfection, the expression of VEGF165 in MCF-7 cells was detected by reverse transcription-quantitative polymerase chain reaction (RT-qPCR) and western blotting...
March 2017: Oncology Letters
https://www.readbyqxmd.com/read/28454275/variations-in-the-multimerization-region-of-the-helicobacter-pylori-cytotoxin-caga-affect-virulence
#13
Daiva Ahire, Tricia Alston, Robert Raffaniello
Helicobacter pylori colonizes the human stomach by infecting gastric epithelial cells. It is the primary cause of peptic ulcer disease and gastric cancer (GC). Cytotoxin-associated gene A (CagA) is a virulence factor produced by H. pylori. Strains positive for the CagA protein are associated with more severe gastric diseases. The 3' region of the cagA gene exhibits heterogeneity with respect to tyrosine phosphorylation motifs (EPIYA) and CagA multimerization motifs (CM). CagA proteins are categorized as either Western or Eastern based on EPIYA sequences...
March 2017: Oncology Letters
https://www.readbyqxmd.com/read/28454265/downregulation-of-rasal2-promotes-the-proliferation-epithelial-mesenchymal-transition-and-metastasis-of-colorectal-cancer-cells
#14
Zeming Jia, Weidong Liu, Liansheng Gong, Zhongfu Xiao
RAS protein activator like 2 (RASAL2) is a RAS-GTPase-activating protein and has recently been identified to be a tumor suppressor in various types of human cancer; however, the function of RASAL2 in colorectal carcinoma (CRC) remains unclear. In the present study, the function of RASAL2 in CRC cells was investigated using a RASAL2 loss-of-function cell model. RASAL2 short hairpin RNA was transfected into the human CRC cell lines LoVo, SW620 and HCT116, and the wild-type colon cell line NCM460. The subsequent downregulation of RASAL2 was evaluated using western blot and reverse transcription-quantitative polymerase chain reaction analyses...
March 2017: Oncology Letters
https://www.readbyqxmd.com/read/28454264/luteolin-a-novel-p90-ribosomal-s6-kinase-inhibitor-suppresses-proliferation-and-migration-in-leukemia-cells
#15
Lan Deng, Ling Jiang, Xianghua Lin, Kuo-Fu Tseng, Zhigang Lu, Xiuju Wang
Ribosomal S6 kinases (RSKs) are directly regulated by extracellular signal-regulated kinase (ERK) signaling and are implicated in cell growth, survival, motility and senescence. The present study observed that RSK1 was overexpressed in primary untreated leukemia patient bone marrow samples compared with the expression at the complete remission stage, using reverse transcription-quantitative polymerase chain reaction (RT-qPCR). In addition, a high RSK1 expression (relative expression ≥10) was associated with a significantly shorter overall survival (P=0...
March 2017: Oncology Letters
https://www.readbyqxmd.com/read/28454247/ampd3-is-associated-with-the-malignant-characteristics-of-gastrointestinal-stromal-tumors
#16
Meihong Wong, Kohei Funasaka, Tomohiko Obayashi, Ryoji Miyahara, Yoshiki Hirooka, Michinari Hamaguchi, Hidemi Goto, Takeshi Senga
Gastrointestinal stromal tumors (GISTs) are mesenchymal tumors of the gastrointestinal tract. It is well known that activating mutations in the receptor tyrosine kinases KIT and platelet-derived growth factor receptor-α have essential roles in the pathogenesis of GISTs. The activation of these receptor protein kinases triggers multiple signaling pathways that promote cell proliferation and survival; however, the exact mechanism by which the activation of these kinases promotes the progression of GISTs remains uncertain...
March 2017: Oncology Letters
https://www.readbyqxmd.com/read/28454241/transthyretin-regulates-the-migration-and-invasion-of-jeg-3-cells
#17
Liyun Gong, Lei Zhu, Shuzhen Wang, Zhenyu Zhang
Preeclampsia (PE) is a pregnancy-specific disorder characterized by new-onset hypertension and proteinuria that occurs after 20 weeks of gestation. It involves several organs and continues to be a leading cause of maternal and perinatal morbidity and mortality worldwide. Shallow trophoblast invasion is a common pathological feature of PE. Transthyretin (TTR) is a 56-kDa homotetrameric protein that binds thyroid hormone and retinol binding protein. Dysregulated TTR expression has been found in cases of PE. The aim of the present study was to determine the functional role of TTR in the migration and invasion of JEG-3 choriocarcinoma cells...
March 2017: Oncology Letters
https://www.readbyqxmd.com/read/28454226/long-non-coding-rna-hotair-enhances-radioresistance-in-mda-mb231-breast-cancer-cells
#18
Yun Zhou, Chaoqun Wang, Xia Liu, Chengjun Wu, Haitao Yin
The aim of the present study was to investigate the radiosensitizing effects of homeobox (HOX) transcript antisense RNA (HOTAIR) long non-coding RNA on breast cancer tumor cells and examine the underlying mechanisms. Recombinant plasmid vectors containing HOTAIR gene were constructed and MDA-MB231 cells were transfected with these plasmids using liposomes. The cells were treated with radiation and cell apoptosis, proliferation, and double-stranded DNA breaks were examined. HOXD10, phosphorylated AKT (p-AKT) and p-BAD expression levels were measured using western blot analysis...
March 2017: Oncology Letters
https://www.readbyqxmd.com/read/28454222/effects-of-rna-silencing-of-matrix-metalloproteinase-2-on-the-growth-of-esophageal-carcinoma-cells-in-vivo
#19
Yu-Guang Shen, Wen Feng, Yi-Jun Xu, Na-Na Jiao, Da-Qiang Sun, Wen-Dong Qu, Quan Tang, Wei Xiong, Yang Tang, Yu Xia, Qing-Yong Cai, Da-Xing Liu, Xun Zhang, Gang Xu, Gui-You Liang
Esophageal carcinoma is one of the most common malignancies in China. Previous studies reported that matrix metalloproteinases (MMPs) have important roles in the progression and invasion of numerous types of solid tumors. Among the MMPs, MMP-2 has been closely associated with tumor growth and invasion. In the present study, a short hairpin RNA (shRNA) lentiviral expression vector targeting the MMP-2 gene was constructed in order to observe the inhibitory effect of MMP-2 gene silencing on the growth of the KYSE150 esophageal carcinoma cell line in vivo...
March 2017: Oncology Letters
https://www.readbyqxmd.com/read/28454118/simultaneous-exposure-to-fc%C3%AE-r-and-fc%C3%AE-r-on-monocytes-and-macrophages-enhances-antitumor-activity-in-vivo
#20
Bingyu Li, Lijun Xu, Fei Tao, Kun Xie, Zhiqiang Wu, You Li, Jie Li, Kaiming Chen, Chenyu Pi, Andrew Mendelsohn, James W Larrick, Hua Gu, Jianmin Fang
Therapeutic antibodies are effective for tumor immunotherapy and exhibit prominent clinical effects. All approved antibody therapeutics utilize IgG as the molecular format. Antibody-dependent cell-mediated cytotoxicity (ADCC) is a key mechanism for tumor cell killing by antibodies. For IgG antibodies, ADCC depends on FcγR-expressing cells, such as natural killer (NK) cells. However, in patients with a high tumor burden, antibody therapeutics may lose efficacy owing to exhaustion of FcγR-expressing effector cells as well as the inhibitory effects of certain FcγRs on effector cells...
April 10, 2017: Oncotarget
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