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https://www.readbyqxmd.com/read/29030872/survival-and-detection-of-bacillus-cereus-in-the-presence-of-escherichia-coli-salmonella-enteriditis-staphylococcus-aureus-pseudomonas-aeruginosa-and-candida-albicans-after-rechallenge-in-makeup-removers
#1
Nadine Yossa, Gabriela Arce, James Smiley, Mei-Chiung Jo Huang, Lanlan Yin, Rebecca Bell, Sandra Tallent, Eric Brown, Thomas Hammack
OBJECTIVE: Pathogenic contamination of cosmetics intended to be applied on or around the eye area, including make-up removers, may lead to severe eye infections. To assess the efficacy of antimicrobial preservatives in these products we investigated the survival and detection of Bacillus cereus F 4227A spiked into make-up removers, alone and in the presence of other relevant microorganisms. METHODS: Four brands of make-up removers, A, B, C and D, were challenged three times (day 0, day 7, and day 14) using B...
October 14, 2017: International Journal of Cosmetic Science
https://www.readbyqxmd.com/read/28977678/associations-between-resistance-phenotype-and-gene-expression-in-response-to-serial-exposure-to-oxacillin-and-ciprofloxacin-in-staphylococcus-aureus
#2
Md Jalal Uddin, Juhee Ahn
This study was designed to delineate the relationship between resistance phenotypes and gene expression in wild-type (SA(WT) ), oxacillin-induced (SA(OXA) ), ciprofloxacin-induced (SA(CIP) ), and clinically-acquired antibiotic-resistant Staphylococcus aureus (SA(CA) ) exposed to oxacillin (β-lactam) and ciprofloxacin (fluoroquinolone). The phenotypic response and gene expression were varied with the antibiotic exposure. SA(WT) was highly resistant to oxacillin (MIC=8 μg ml(-1) ) after serial exposure to oxacillin, while the oxacillin susceptibility was not changed in SA(WT) when exposed to ciprofloxacin (MIC=0...
October 4, 2017: Letters in Applied Microbiology
https://www.readbyqxmd.com/read/28949803/enterotoxin-production-and-antimicrobial-susceptibility-in-staphylococci-isolated-from-traditional-raw-milk-cheeses-in-serbia
#3
Snezana Bulajic, Svetlana Colovic, Dusan Misic, Jasna Djordjevic, Radoslava Savic-Radovanovic, Jelena Asanin, Tijana Ledina
This study was undertaken to determine the prevalence of coagulase positive staphylococci (CPS) by examining a total of 71 raw milk cheeses. Additionally, enterotoxigenicity, antimicrobial susceptibility and the presence of mecA and mecC genes in the staphylococcal isolates were investigated. The isolation and enumeration procedure of CPS followed the International Organization for Standardization (ISO) standard. The presumptive staphylococci were identified by matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) using the VITEK MS system...
September 26, 2017: Journal of Environmental Science and Health. Part. B, Pesticides, Food Contaminants, and Agricultural Wastes
https://www.readbyqxmd.com/read/28927452/vancomycin-heteroresistance-in-coagulase-negative-staphylococcus-blood-stream-infections-from-patients-of-intensive-care-units-in-mansoura-university-hospitals-egypt
#4
Ghada El-Saeed Mashaly, Rasha Hassan El-Mahdy
BACKGROUND: Vancomycin heteroresistance in coagulase negative Staphylococci (CoNS) is a recent health concern especially in serious infections like bloodstream infections as it may lead to failure of therapy. Little information is available about the prevalence vancomycin heteroresistance in CoNS causing bloodstream infections in intensive care units (ICUs) patients of Mansoura University Hospitals (MUHs). METHODS: This prospective study enrolled 743 blood samples collected from ICUs patients presented with clinical manifestations of bloodstream infections over the period extending from January 2014 to March 2016...
September 19, 2017: Annals of Clinical Microbiology and Antimicrobials
https://www.readbyqxmd.com/read/28922025/prevalence-of-staphylococcal-enterotoxins-in-ready-to-eat-foods-sold-in-istanbul
#5
Beyza H Ulusoy, Burcu Çakmak Sancar, Muhsin Öztürk
The aim of this study was to investigate the prevalence of staphylococcal enterotoxins (SEs) in ready-to-eat (RTE) foods sold in Istanbul, Turkey. A total of 5,241 samples were randomly collected from various caterers, hotels, and restaurants from 2014 to 2016. The samples were classified into four groups: (i) various cooked RTE meat and vegetable meals, (ii) various RTE salads, charcuterie, and cold appetizers, (iii) various cooked RTE bakery products (pasta, pastries, pizza, pita, ravioli, etc.), and (iv) any cooked RTE sweets and desserts (pudding, custard, cream, ashura, etc...
October 2017: Journal of Food Protection
https://www.readbyqxmd.com/read/28865851/prevalence-antimicrobial-susceptibility-and-molecular-characterization-of-staphylococcus-aureus-isolated-from-dairy-herds-in-northern-china
#6
Huimin Liu, Songli Li, Lu Meng, Lei Dong, Shengguo Zhao, Xinyi Lan, Jiaqi Wang, Nan Zheng
Staphylococcus aureus is one of the main pathogens involved in dairy cow mastitis. Monitoring of antibiotic use would prove useful to assess the risk of Staph. aureus in raw milk. The objective of this work was to investigate the prevalence of Staph. aureus strais isolated from raw milk in northern China, and to characterize antimicrobial susceptibility of these strains and their key virulence genes. In total, 195 raw milk samples were collected from 195 dairy farms located in 4 cities of northern China from May to September 2015...
November 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/28845592/prevalence-of-%C3%AE-lactamase-genes-in-domestic-washing-machines-and-dishwashers-and-the-impact-of-laundering-processes-on-antibiotic-resistant-bacteria
#7
Laura Rehberg, André Frontzek, Åsa Melhus, Dirk P Bockmühl
AIMS: To investigate the prevalence of β-lactamase genes in domestic washing machines and dishwashers and the decontamination efficacy of laundering. METHODS AND RESULTS: In a first investigation, swab-samples from washing machines (n=29) and dishwashers (n=24) were analysed by real-time quantitative PCR to detect genes encoding β-lactamases. To test the impact of laundering on resistant bacteria, cotton test swatches were artificially contaminated with susceptible and resistant strains of Pseudomonas aeruginosa, Klebsiella pneumoniae and Staphylococcus aureus within a second investigation...
August 28, 2017: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/28761643/a-prospective-randomised-trial-of-isolated-pathogens-of-surgical-site-infections-ssi
#8
Konstantinos Alexiou, Ioannis Drikos, Maria Terzopoulou, Nikolaos Sikalias, Argyrios Ioannidis, Nikolaos Economou
BACKGROUND: Every surgical wound is colonized by bacteria, but only a small percentage displays symptoms of infection. The distribution of pathogens isolated in surgical site infections has not significantly changed over the last decades. Staph. Aureus, Coag(-) Staphylococci, Enterococcus spp and E. Coli are the main strains appearing. In addition, a continuously rising proportion of surgical site infections caused by resistant bacterial species (MRSA, C. Albicans) has been reported...
September 2017: Annals of Medicine and Surgery
https://www.readbyqxmd.com/read/28732197/wrecking-staph-s-rafts-staphylococcus-aureus-no-longer-unsinkable
#9
Rafi Rashid, Kimberly A Kline
Functional membrane microdomains (FMMs) serve to spatially restrict and coordinate a diversity of cellular functions. Flotillins serve as scaffolds within FMMs, and in this issue of Cell Chemical Biology, Koch et al. (2017) show that disrupting Staphylococcus aureus scaffolds via small molecules perturbs virulence gene expression and attenuates S. aureus virulence.
July 20, 2017: Cell Chemical Biology
https://www.readbyqxmd.com/read/28624271/coagulase-negative-staphylococci-species-affect-biofilm-formation-of-other-coagulase-negative-and-coagulase-positive-staphylococci
#10
Coralie Goetz, Yannick D N Tremblay, Daphnée Lamarche, Andréanne Blondeau, Annie M Gaudreau, Josée Labrie, François Malouin, Mario Jacques
Coagulase-negative staphylococci (CNS) are considered to be commensal bacteria in humans and animals, but are now also recognized as etiological agents in several infections, including bovine mastitis. Biofilm formation appears to be an important factor in CNS pathogenicity. Furthermore, some researchers have proposed that CNS colonization of the intramammary environment has a protective effect against other pathogens. The mechanisms behind the protective effect of CNS have yet to be characterized. The aim of this study was to evaluate the effect of CNS isolates with a weak-biofilm phenotype on the biofilm formation of other staphylococcal isolates...
June 14, 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/28607765/paediatric-bone-and-joint-infection
#11
REVIEW
Alexios D Iliadis, Manoj Ramachandran
Despite advances in understanding and management, paediatric osteoarticular infections continue to pose diagnostic difficulties for clinicians. Delays in diagnosis can lead to potentially devastating morbidity.No single investigation, including joint aspiration, is sufficiently reliable to diagnose conclusively paediatric bone and joint infection. Diagnosis should be based on a combination of clinical signs, imaging and laboratory investigations. Algorithms should supplement, and not replace, clinical decision making in all cases...
January 2017: EFORT open reviews
https://www.readbyqxmd.com/read/28573492/bactericidal-activity-and-synergy-studies-of-peptide-ap-cect7121-against-multi-resistant-bacteria-isolated-from-human-and-animal-soft-tissue-infections
#12
Gastón Delpech, Mariana Bistoletti, Mónica Ceci, Sabina Lissarrague, Sergio Sánchez Bruni, Mónica Sparo
AP-CECT7121 is an antimicrobial peptide, produced by Enterococcus faecalis CECT7121, with bactericidal activity against Gram-positive bacteria. The aim of this study was to evaluate the bactericidal activity of AP-CECT7121, alone and with gentamicin, against multi-resistant bacteria isolated from human and animals with soft tissue infections. During the period 2014-2015, bacterial strains producing human and animal soft tissue infections were studied. Samples from patients attended at a general hospital and cattle from four dairies in the Province of Buenos Aires (Argentina) were included...
June 1, 2017: Probiotics and Antimicrobial Proteins
https://www.readbyqxmd.com/read/28527807/dna-carryover-in-milk-samples-from-routine-milk-recording-used-for-pcr-based-diagnosis-of-bovine-staphylococcus-aureus-mastitis
#13
Yasser S Mahmmod, Ilka C Klaas, Carsten Enevoldsen
Real-time PCR techniques are increasingly used to detect udder pathogens from milk samples collected non-aseptically at routine milk recording. The objectives of this study were (1) to estimate the statistical associations between cycle threshold (Ct) values for Staphylococcus aureus in non-aseptically collected composite samples taken at routine milk recording from cows milked consecutively with the same milking unit and milk meter; and (2) to formulate practical and plausible guidelines for understanding the diagnostic implications of PCR testing for Staph...
July 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/28450213/isolation-characterization-virulence-and-immunogenicity-testing-of-field-isolates-of-pasteurella-multocida-staphylococcus-aureus-and-streptococcus-agalactiae-in-laboratory-settings
#14
Qudratullah, G Muhammad, M Saqib, M Qamar Bilal
The present study was designed to investigate isolation, characterization, virulence and immunogenicity testing of field isolates of Pasteurella multocida, Staphylococcus aureus, and Streptococcus agalactiae in rabbits and mice. Isolates of P. multocida, S. aureus and Str. agalactiae recovered from field cases of Hemorragic septicemia and mastitis were scrutinized for virulence/pathogenicity and immunogenicity. Mouse LD50 of P. multocida showed that P. multocida isolate No.1 was more virulent than isolates No...
April 24, 2017: Acta Tropica
https://www.readbyqxmd.com/read/28434738/relationships-among-superantigen-toxin-gene-profiles-genotypes-and-pathogenic-characteristics-of-staphylococcus-aureus-isolates-from-bovine-mastitis
#15
Dong Wang, Limei Zhang, Changfu Yong, Mingliang Shen, Tariq Ali, Muhammad Shahid, Kun Han, Xuezhang Zhou, Bo Han
Staphylococcus aureus is one of the major etiological agents of bovine mastitis, harboring a wide variety of staphylococcal superantigen (SAg) toxin genes. The SAg toxin genes are reported to be closely associated with the pathogenicity of the Staph. aureus causing the bovine mastitis. This study was conducted to investigate SAg toxin gene profiles and to assess the relationships among SAg toxin genes, genotypes of Staph. aureus, and their pathogenic properties. A total of 327 quarter milk samples were collected from bovine mastitis cases for isolation and identification of pathogens...
April 20, 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/28381601/novel-multiplex-pcr-assay-for-detection-of-chlorhexidine-quaternary-ammonium-mupirocin-and-methicillin-resistance-genes-with-simultaneous-discrimination-of-staphylococcus-aureus-from-coagulase-negative-staphylococci
#16
Jo-Ann McClure, Johanna Zaal DeLongchamp, John M Conly, Kunyan Zhang
Methicillin-resistant Staphylococcus aureus (MRSA) is a clinically significant pathogen that is resistant to a wide variety of antibiotics and responsible for a large number of nosocomial infections worldwide. The Agency for Healthcare Research and Quality and the Centers for Disease Control and Prevention recently recommended the adoption of universal mupirocin-chlorhexidine decolonization of all admitted intensive care unit patients rather than MRSA screening with targeted treatments, which raises a serious concern about the selection of resistance to mupirocin and chlorhexidine in strains of staphylococci...
June 2017: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/28373644/modeling-nosocomial-infections-of-methicillin-resistant-staphylococcus-aureus-with-environment-contamination
#17
Lei Wang, Shigui Ruan
In this work, we investigate the role of environmental contamination on the clinical epidemiology of antibiotic-resistant bacteria in hospitals. Methicillin-resistant Staphylococcus aureus (MRSA) is a bacterium that causes infections in different parts of the body. It is tougher to treat than most strains of Staphylococcus aureus or staph, because it is resistant to some commonly used antibiotics. Both deterministic and stochastic models are constructed to describe the transmission characteristics of MRSA in hospital setting...
April 3, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28341052/factors-associated-with-intramammary-infection-in-dairy-cows-caused-by-coagulase-negative-staphylococci-staphylococcus-aureus-streptococcus-uberis-streptococcus-dysgalactiae-corynebacterium-bovis-or-escherichia-coli
#18
S Taponen, E Liski, A-M Heikkilä, S Pyörälä
The aim of this study was to determine risk factors for bovine intramammary infection (IMI) associated with the most common bacterial species in Finland. Large databases of the Finnish milk-recording system and results of microbiological analyses of mastitic milk samples from Valio Ltd. (Helsinki, Finland) were analyzed. The study group comprised 29,969 cows with IMI from 4,173 dairy herds. A cow with a quarter milk sample in which DNA of target species was detected in the PathoProof Mastitis PCR Assay (Thermo Fisher Scientific, Waltham, MA) was determined to have IMI...
January 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/28314156/an-explant-of-heifer-mammary-gland-to-study-the-immune-response-of-the-organ
#19
Giada Magro, Tiziana A L Brevini, Marcella De Maglie, Giulietta Minozzi, Eugenio Scanziani, Renata Piccinini
Continuous or primary epithelial cell lines have been extensively used to study the mammary gland immune response, but they are constituted by a single cell population. Our aim was to test whether an explant of heifer gland, where the tissue structure is maintained, might be a valid model to investigate the innate immune response to infection. The study was carried out on 2mm(3)-sections of heifer udders, in 2 consecutive trials, using LPS or LTA in the first trial and two different concentrations of Staphylococcus aureus (Staph...
March 6, 2017: Research in Veterinary Science
https://www.readbyqxmd.com/read/28274250/prosthetic-joint-infection-development-of-an-evidence-based-diagnostic-algorithm
#20
Heinrich M L Mühlhofer, Florian Pohlig, Karl-Georg Kanz, Ulrich Lenze, Florian Lenze, Andreas Toepfer, Sarah Kelch, Norbert Harrasser, Rüdiger von Eisenhart-Rothe, Johannes Schauwecker
BACKGROUND: Increasing rates of prosthetic joint infection (PJI) have presented challenges for general practitioners, orthopedic surgeons and the health care system in the recent years. The diagnosis of PJI is complex; multiple diagnostic tools are used in the attempt to correctly diagnose PJI. Evidence-based algorithms can help to identify PJI using standardized diagnostic steps. METHODS: We reviewed relevant publications between 1990 and 2015 using a systematic literature search in MEDLINE and PUBMED...
March 9, 2017: European Journal of Medical Research
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