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https://www.readbyqxmd.com/read/29777963/red-wolf-canis-rufus-sperm-quality-and-quantity-is-affected-by-semen-collection-method-extender-components-and-post-thaw-holding-temperature
#1
Ashley D Franklin, William T Waddell, Karen L Goodrowe
Cryopreserving genetic resources is becoming increasingly important for species management. In the zoo-based red wolf (Canis rufus) population, inbreeding continues to increase in the absence of new founders. Through banking sperm, we preserve genetic diversity and create the ability to decrease inbreeding accumulation in the future. The quality and quantity of banked sperm can be affected by cryopreservation media and semen collection methods. This study's objectives were to further optimize semen extender used for red wolf sperm cryopreservation, investigate effects of post-thaw holding temperature, and to determine if urethral catheterization is an effective method for semen collection in this species...
May 16, 2018: Theriogenology
https://www.readbyqxmd.com/read/29771630/septins-regulate-junctional-integrity-of-endothelial-monolayers
#2
Joanna Kim, John A Cooper
Junctional integrity of endothelial monolayers is crucial to control movement of molecules and cells across the endothelium. Examining the structure and dynamics of cell junctions in endothelial monolayers, we discovered a role for septins. Contacts between adjacent endothelial cells were dynamic, with protrusions extending above or below neighboring cells. VE-cadherin was present at cell junctions, with a membrane-associated layer of F-actin. Septins localized at cell-junction membranes, in patterns distinct from VE-cadherin and F-actin...
May 17, 2018: Molecular Biology of the Cell
https://www.readbyqxmd.com/read/29760772/engineering-a-palette-of-eukaryotic-chromoproteins-for-bacterial-synthetic-biology
#3
Josefine Liljeruhm, Saskia K Funk, Sandra Tietscher, Anders D Edlund, Sabri Jamal, Pikkei Wistrand-Yuen, Karl Dyrhage, Arvid Gynnå, Katarina Ivermark, Jessica Lövgren, Viktor Törnblom, Anders Virtanen, Erik R Lundin, Erik Wistrand-Yuen, Anthony C Forster
Background: Coral reefs are colored by eukaryotic chromoproteins (CPs) that are homologous to green fluorescent protein. CPs differ from fluorescent proteins (FPs) by intensely absorbing visible light to give strong colors in ambient light. This endows CPs with certain advantages over FPs, such as instrument-free detection uncomplicated by ultra-violet light damage or background fluorescence, efficient Förster resonance energy transfer (FRET) quenching, and photoacoustic imaging. Thus, CPs have found utility as genetic markers and in teaching, and are attractive for potential cell biosensor applications in the field...
2018: Journal of Biological Engineering
https://www.readbyqxmd.com/read/29760272/physical-activity-assessment-and-counseling-in-quebec-family-medicine-groups
#4
Aurélie Baillot, Jean-Patrice Baillargeon, Alex Paré, Thomas G Poder, Christine Brown, Marie-France Langlois
OBJECTIVE: To determine how often primary health care providers (PHCPs) in family medicine groups (FMGs) assess physical activity (PA) levels, provide PA counseling (PAC), and refer patients to exercise professionals; to describe patients' PA levels, physical fitness, and satisfaction regarding their PA management in FMGs; to describe available PA materials in FMGs and PHCPs' PAC self-efficacy and PA knowledge; and to identify characteristics of patients and PHCPs that determine the assessment of PA and PAC provided by PHCPs...
May 2018: Canadian Family Physician Médecin de Famille Canadien
https://www.readbyqxmd.com/read/29760266/the-reality-of-urban-fps
#5
Francine Lemire
No abstract text is available yet for this article.
May 2018: Canadian Family Physician Médecin de Famille Canadien
https://www.readbyqxmd.com/read/29757279/engineering-golden-fluorescence-by-selective-pressure-incorporation-of-non-canonical-amino-acids-and-protein-analysis-by-mass-spectrometry-and-fluorescence
#6
Tobias Baumann, Franz-Josef Schmitt, Almut Pelzer, Vivian Jeanette Spiering, Georg Johannes Freiherr von Sass, Thomas Friedrich, Nediljko Budisa
Fluorescent proteins are fundamental tools for the life sciences, in particular for fluorescence microscopy of living cells. While wild-type and engineered variants of the green fluorescent protein from Aequorea victoria (avGFP) as well as homologs from other species already cover large parts of the optical spectrum, a spectral gap remains in the near-infrared region, for which avGFP-based fluorophores are not available. Red-shifted fluorescent protein (FP) variants would substantially expand the toolkit for spectral unmixing of multiple molecular species, but the naturally occurring red-shifted FPs derived from corals or sea anemones have lower fluorescence quantum yield and inferior photo-stability compared to the avGFP variants...
April 27, 2018: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/29749485/gas%C3%A2-filled-ultrasound-microbubbles-enhance-the-immunoactivity-of-the-hsp70%C3%A2-magea1-fusion-protein-against-magea1%C3%A2-expressing-tumours
#7
Xing Gao, Yang Nan, Yuan Yuan, Xue Gong, Yuanyuan Sun, Huihui Zhou, Yujin Zong, Lijun Zhang, Ming Yu
Advanced malignant melanoma is characterized by rapid development, poor prognosis and insensitivity to chemoradiotherapy. Immunotherapy has become one of the primary clinical treatments for malignant melanomas. In recent decades, identifying specific tumour antigens and the enhanced immunoactivity of tumour vaccines has become critical for engineering successful tumour vaccines. As a widely used vaccine carrier, heat shock protein 70 (HSP70) clearly increases the immunogenicity of tumour antigens, such as melanoma‑associated antigen A1 (MAGEA1)...
May 9, 2018: Molecular Medicine Reports
https://www.readbyqxmd.com/read/29747061/serrated-epithelial-colorectal-polyps-hyperplastic-polyps-sessile-serrated-adenomas-with-perineurial-stroma-clinicopathological-and-molecular-analysis-of-a-new-series
#8
Katharina Erlenbach-Wünsch, Michel Bihl, Arndt Hartmann, Gabriel M Groisman, Michael Vieth, Abbas Agaimy
Serrated colorectal fibroblastic polyps (FPs) are rare benign mucosal lesions composed of serrated epithelial crypts separated and distorted by intimately associated bland spindle cell proliferations with perineurial-like phenotype. We herein describe 21 new FPs affecting 10 females and 9 males aged 45 to 80 yrs. (mean, 62 yrs). Lesions originated in the sigmoid colon/rectosigmoid junction (n = 16), rectum (n = 2), and other parts of the colon (n = 3). Most patients had additional synchronous or metachronous polyps: classical adenomas (12 patients), sessile serrated adenoma/SSA (1 patient), hyperplastic polyps/HPs (7 patients), both HPs and adenomas (6 patients) and colorectal cancer (2 patients)...
May 4, 2018: Annals of Diagnostic Pathology
https://www.readbyqxmd.com/read/29747014/development-of-a-national-cosmic-ray-dose-monitoring-system-with-health-canada-s-fixed-point-surveillance-network
#9
Chuanlei Liu, Weihua Zhang, Kurt Ungar, Ed Korpach, Brian White, Mike Benotto, Eric Pellerin
This work explores the application of Health Canada's Fixed Point Surveillance (FPS) network for cosmic ray monitoring and dose estimation purposes. This network is comprised of RS250 3 inch by 3 inch Sodium Iodide (NaI) spectroscopic dosimeters distributed throughout Canada. The RS250's high channel count rate responds to the electromagnetic and muonic components of cosmic ray shower. These count rates are used to infer cosmic ray doses throughout FPS locations. The derived dose was found to have an accuracy within 6...
May 7, 2018: Journal of Environmental Radioactivity
https://www.readbyqxmd.com/read/29739943/designer-cells-programming-quorum-sensing-interference-with-microbes
#10
Ferdinand Sedlmayer, Dennis Hell, Marius Müller, David Ausländer, Martin Fussenegger
Quorum sensing is a promising target for next-generation anti-infectives designed to address evolving bacterial drug resistance. The autoinducer-2 (AI-2) is a key quorum-sensing signal molecule which regulates bacterial group behaviors and is recognized by many Gram-negative and Gram-positive bacteria. Here we report a synthetic mammalian cell-based microbial-control device that detects microbial chemotactic formyl peptides through a formyl peptide sensor (FPS) and responds by releasing AI-2. The microbial-control device was designed by rewiring an artificial receptor-based signaling cascade to a modular biosynthetic AI-2 production platform...
May 8, 2018: Nature Communications
https://www.readbyqxmd.com/read/29736114/exploring-education-and-training-needs-in-palliative-care-among-family-physicians-in-mumbai-a-qualitative-study
#11
Anuja Damani, Arunangshu Ghoshal, Manjiri Dighe, Sunil Dhiliwal, Maryann Muckaden
Context: Patients with chronic life-limiting conditions on palliative care (PC) prefer to be treated at home. Medical care by family physicians (FPs) reduces demand on costly and busy hospital facilities. Working of PC team in collaboration with FPs is thus helpful in home-based management of patients. Aims: This study aimed at exploring the extent of knowledge of FPs about PC and the need for additional training. Settings and Design: Semi-structured interviews were conducted with ten FPs from two suburbs of Mumbai, currently served by home care services of a tertiary cancer care center...
April 2018: Indian Journal of Palliative Care
https://www.readbyqxmd.com/read/29715165/implementation-of-a-low-frame-rate-protocol-and-noise-reduction-technology-to-minimize-radiation-dose-in-transcatheter-aortic-valve-replacement
#12
Davide Maccagni, Luciano Candilio, Azeem Latib, Cosmo Godino, Alaide Chieffo, Matteo Montorfano, Antonio Colombo, Lorenzo Azzalini
OBJECTIVES: Limiting radiation exposure is necessary in radiological procedures. This study evaluates the impact of a radiological low frame-rate protocol in a standard angiographic system and the implementation of a noise-reduction technology (NRT) on patient radiation exposure during transcatheter aortic valve replacement (TAVR). METHODS: Transfemoral TAVR procedures performed between February 2016 and February 2017 were analyzed according to two angiographic systems, Standard and NRT, and further divided in four subgroups: (1) Standard 15 frames per second (fps) with 15 fps for both fluoroscopy and cine acquisitions; (2) Standard 7...
May 2018: Journal of Invasive Cardiology
https://www.readbyqxmd.com/read/29710591/deactivation-of-wastewater-derived-n-nitrosodimethylamine-precursors-with-chlorine-dioxide-oxidation-and-the-effect-of-ph
#13
Habibullah Uzun, Daekyun Kim, Tanju Karanfil
In this study, the effect of chlorine dioxide (ClO2 ) oxidation on the deactivation of wastewater (WW)-derived N-nitrosodimethylamine (NDMA) precursors was investigated under various conditions (i.e., ClO2 application pH, dose and contact time). At pH 6.0, decreases in NDMA formation potentials (FPs) or occurrences (under uniform formation conditions [UFC]) were relatively low (<25%) with ClO2 oxidation regardless of WW-impact. A negative removal was also observed after ClO2 oxidation in some of the non-impacted waters...
April 24, 2018: Science of the Total Environment
https://www.readbyqxmd.com/read/29706254/mammography-clinical-image-quality-and-the-false-positive-rate-in-a-canadian-breast-cancer-screening-program
#14
Marie-Hélène Guertin, Isabelle Théberge, Hervé Tchala Vignon Zomahoun, Michel-Pierre Dufresne, Éric Pelletier, Jacques Brisson
PURPOSE: The study sought to determine if mammography quality is associated with the false positive (FP) rate in the Quebec breast cancer screening program in 2004 and 2005. METHODS: Mammography quality of a random sample of screen-film mammograms was evaluated by an expert radiologist following the criteria of the Canadian Association of Radiologists. For each screening examination, scores ranging from 1 (poor quality) to 5 (excellent quality) were attributed for positioning, compression, contrast, exposure level, sharpness, and artifacts...
May 2018: Canadian Association of Radiologists Journal, Journal L'Association Canadienne des Radiologistes
https://www.readbyqxmd.com/read/29706225/photophysical-behavior-of-mneongreen-an-evolutionarily-distant-green-fluorescent-protein
#15
Frederik Steiert, Eugene P Petrov, Peter Schultz, Petra Schwille, Thomas Weidemann
Fluorescent proteins (FPs) feature complex photophysical behavior that must be considered when studying the dynamics of fusion proteins in model systems and live cells. In this work, we characterize mNeonGreen (mNG), a recently introduced FP from the bilaterian Branchiostoma lanceolatum, in comparison to the well-known hydrozoan variants enhanced green fluorescent protein (EGFP) and Aequorea coerulescens GFP by steady-state spectroscopy and fluorescence correlation spectroscopy in solutions of different pH...
April 26, 2018: Biophysical Journal
https://www.readbyqxmd.com/read/29705968/performance-validation-of-an-amplicon-based-targeted-next-generation-sequencing-assay-and-mutation-profiling-of-648-chinese-colorectal-cancer-patients
#16
Yajian Wang, Haijing Liu, Yingyong Hou, Xiaoyan Zhou, Li Liang, Zhihong Zhang, Huaiyin Shi, Sanpeng Xu, Peizhen Hu, Zuyu Zheng, Rui Liu, Tingdong Tang, Feng Ye, Zhiyong Liang, Hong Bu
Next-generation sequencing (NGS) has become a promising approach for tumor somatic mutation detection. However, stringent validation is required for its application on clinical specimens, especially for low-quality formalin-fixed paraffin-embedded (FFPE) tissues. Here, we validated the performance of an amplicon-based targeted NGS assay, OncoAim™ DNA panel, on both commercial reference FFPE samples and clinical FFPE samples of Chinese colorectal cancer (CRC) patients. Then we profiled the mutation spectrum of 648 Chinese CRC patients in a multicenter study to explore its clinical utility...
April 28, 2018: Virchows Archiv: An International Journal of Pathology
https://www.readbyqxmd.com/read/29704328/complex-regression-doppler-optical-coherence-tomography
#17
Sahar Elahi, Shi Gu, Lars Thrane, Andrew Rollins, Michael Jenkins
We introduce a new method to measure Doppler shifts more accurately and extend the dynamic range of Doppler optical coherence tomography (OCT). The two-point estimate of the conventional Doppler method is replaced with a regression that is applied to high-density B-scans in polar coordinates. We built a high-speed OCT system using a 1.68-MHz Fourier domain mode locked laser to acquire high-density B-scans (16,000 A-lines) at high enough frame rates (∼100  fps) to accurately capture the dynamics of the beating embryonic heart...
April 2018: Journal of Biomedical Optics
https://www.readbyqxmd.com/read/29695748/ridge-furrow-with-plastic-film-and-straw-mulch-increases-water-availability-and-wheat-production-on-the-loess-plateau
#18
Gaoyuan Liu, Yuhuan Zuo, Qi Zhang, Lili Yang, Erlong Zhao, Lianyou Liang, Yan' An Tong
Mulching is critical for increasing water availability and hence winter wheat production in dryland farming systems. A two-year study was conducted to assess the effects of mulches on soil water storage (SWS), temperature, water use efficiency (WUE) and yields of winter wheat on the Loess Plateau. Four treatments were examined: conventional flat planting (CK), straw mulch (FPS), transparent plastic film mulch (FPP) and ridge-furrow with plastic film-mulched ridge and straw-mulched furrow (RFPS). Compared with CK, RFPS greatly increased SWS from 0-60 cm, FPP increased SWS from 0-40 cm, and FPS slightly increased SWS from 0-60 cm; however, FPP significantly (P < 0...
April 25, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29695100/design-and-performance-of-a-1-ms-high-speed-vision-chip-with-3d-stacked-140-gops-column-parallel-pes-%C3%A2
#19
Atsushi Nose, Tomohiro Yamazaki, Hironobu Katayama, Shuji Uehara, Masatsugu Kobayashi, Sayaka Shida, Masaki Odahara, Kenichi Takamiya, Shizunori Matsumoto, Leo Miyashita, Yoshihiro Watanabe, Takashi Izawa, Yoshinori Muramatsu, Yoshikazu Nitta, Masatoshi Ishikawa
We have developed a high-speed vision chip using 3D stacking technology to address the increasing demand for high-speed vision chips in diverse applications. The chip comprises a 1/3.2-inch, 1.27 Mpixel, 500 fps (0.31 Mpixel, 1000 fps, 2 &times; 2 binning) vision chip with 3D-stacked column-parallel Analog-to-Digital Converters (ADCs) and 140 Giga Operation per Second (GOPS) programmable Single Instruction Multiple Data (SIMD) column-parallel PEs for new sensing applications. The 3D-stacked structure and column parallel processing architecture achieve high sensitivity, high resolution, and high-accuracy object positioning...
April 24, 2018: Sensors
https://www.readbyqxmd.com/read/29694432/enhanced-production-of-polysaccharides-and-triterpenoids-in-ganoderma-lucidum-fruit-bodies-on-induction-with-signal-transduction-during-the-fruiting-stage
#20
Liyun Ye, Shengrong Liu, Fan Xie, Lili Zhao, Xiaoping Wu
Ganoderma lucidum is a medicinal mushroom that has been widely used in East Asia for the treatment of various diseases. The pharmacological activity of this fungus is primarily attributable to the polysaccharides and triterpenoids. In this study, to obtain the fruit bodies with improved content of active constituents, we examined the effect of salicylic acid (SA) and calcium ion on the biosynthesis of polysaccharides and triterpenoids by spraying the chemicals during the fruiting. To explore the underlying mechanisms for the variation, the transcripts of related genes involved in the polysaccharide and triterpenoid biosynthesis were measured...
2018: PloS One
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