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https://www.readbyqxmd.com/read/28936332/designing-brighter-near-infrared-fluorescent-proteins-insights-from-structural-and-biochemical-studies
#1
Mikhail Baloban, Daria M Shcherbakova, Sergei Pletnev, Vladimir Z Pletnev, J Clark Lagarias, Vladislav V Verkhusha
Brighter near-infrared (NIR) fluorescent proteins (FPs) are required for multicolor microscopy and deep-tissue imaging. Here, we present structural and biochemical analyses of three monomeric, spectrally distinct phytochrome-based NIR FPs, termed miRFPs. The miRFPs are closely related and differ by only a few amino acids, which define their molecular brightness, brightness in mammalian cells, and spectral properties. We have identified the residues responsible for the spectral red-shift, revealed a new chromophore bound simultaneously to two cysteine residues in the PAS and GAF domains in blue-shifted NIR FPs, and uncovered the importance of amino acid residues in the N-terminus of NIR FPs for their molecular and cellular brightness...
June 1, 2017: Chemical Science
https://www.readbyqxmd.com/read/28935867/s100a4-contributes-to-colitis-development-by-increasing-the-adherence-of-citrobacter-rodentium-in-intestinal-epithelial-cells
#2
Jinhua Zhang, Ying Jiao, Shasha Hou, Tian Tian, Qi Yuan, Huaijie Hao, Zhenlong Wu, Xuexiang Bao
S100A4 has been implicated in cancer and several inflammatory diseases, but its role in inflammatory bowel disease has not been well investigated. Here, upon infection with Citrobacter rodentium, a model for enteropathogenic Escherichia coli infection in humans, induced the infiltration of a large number of S100A4(+) cells into the colon in wild type (WT) mice. Deficiency of S100A4 reduced weight loss, bacterial colonization and colonic pathology. Furthermore, the expression of inflammatory cytokines and the recruitment of macrophages and neutrophils also decreased significantly in S100A4 knock out (S100A4 (-/-)) mice...
September 21, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28931523/contribution-of-myo-inositol-oxygenase-in-age-rage-mediated-renal-tubulo-interstitial-injury-in-the-context-of-diabetic-nephropathy
#3
Isha Sharma, Rashmi S Tupe, Aryana K Wallner, Yashpal S Kanwar
Advanced glycation end products (AGEs) play a role in pathogenesis of diabetic nephropathy (DN). Myo-inositol Oxygenase (MIOX) has been implicated in tubulo-interstitial injury in the context of DN. We investigated the effect of AGEs on MIOX expression and delineated mechanisms that lead to tubulo-interstitial injury. Status of MIOX, RAGE and relevant cellular signaling pathways activated following AGE:RAGE interaction were examined in tubular cells and kidneys of AGE-BSA treated mice. Solid phase assay revealed an enhanced binding of RAGE with AGE-BSA, -laminin and -collagen IV...
September 20, 2017: American Journal of Physiology. Renal Physiology
https://www.readbyqxmd.com/read/28924008/primary-generators-of-visually-evoked-field-potentials-recorded-in-the-macaque-auditory-cortex
#4
Yoshinao Kajikawa, John F Smiley, Charles E Schroeder
Prior studies have reported "local" field potential (LFP) responses to faces in the macaque auditory cortex and have suggested that such face-LFPs may be substrates of audiovisual integration. However, while field potentials (FPs) may reflect the synaptic currents of neurons near the recording electrode, due to the use of a distant reference electrode, they often reflect those of synaptic activity occurring in distant sites as well. Thus, FP recordings within a given brain region (e.g., auditory cortex) may be "contaminated" by activity generated elsewhere in the brain...
September 18, 2017: Journal of Neuroscience: the Official Journal of the Society for Neuroscience
https://www.readbyqxmd.com/read/28915265/the-accessory-parotid-gland-and-facial-process-of-the-parotid-gland-on-computed-tomography
#5
Dongbin Ahn, Chang Ki Yeo, Soon Yong Han, Jeong Kyu Kim
The purpose of this study was to determine the incidence of an anterior extension of the parotid gland, such as an accessory parotid gland (APG) or facial process (FP) and to evaluate its characteristics on computed tomography (CT) scans. We reviewed CT scans of 1,600 parotid glands from 800 patients. An APG on CT was defined as a soft-tissue mass of the same density as the main parotid gland, located at the anterior part of the main parotid gland, and completely separate from the main parotid gland. An FP was defined as a lobe of the parotid gland protruding anteriorly over the anterior edge of the ramus of the mandible on CT and showing continuity with the main gland...
2017: PloS One
https://www.readbyqxmd.com/read/28912565/real-time-image-processing-for-microscopy-based-label-free-imaging-flow-cytometry-in-a-microfluidic-chip
#6
Young Jin Heo, Donghyeon Lee, Junsu Kang, Keondo Lee, Wan Kyun Chung
Imaging flow cytometry (IFC) is an emerging technology that acquires single-cell images at high-throughput for analysis of a cell population. Rich information that comes from high sensitivity and spatial resolution of a single-cell microscopic image is beneficial for single-cell analysis in various biological applications. In this paper, we present a fast image-processing pipeline (R-MOD: Real-time Moving Object Detector) based on deep learning for high-throughput microscopy-based label-free IFC in a microfluidic chip...
September 14, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28910744/advanced-glycation-end-products-induced-il-6-and-vegf-a-production-and-apoptosis-in-osteocyte-like-mlo-y4-cells-by-activating-rage-and-erk1-2-p38-and-stat3-signalling-pathways
#7
Helin Chen, Wenjia Liu, Xiangnan Wu, Min Gou, Jiefei Shen, Hang Wang
Advanced glycation end products (AGEs) are involved in osteopenia in people with diabetes and the elderly. Interleukin-6 (IL-6) and vascular endothelial growth factor-A (VEGF-A) are potent regulators of bone metabolism, and in bone tissue, osteocytes are an important source of these regulators. However, whether AGEs can directly regulate IL-6 and VEGF-A secretion by osteocytes is unknown. In this study, we evaluated the effect of AGEs on IL-6 and VEGF- A production as well as apoptosis in osteocyte-like MLO-Y4 cells...
September 11, 2017: International Immunopharmacology
https://www.readbyqxmd.com/read/28904048/what-can-organizations-do-to-improve-family-physicians-interprofessional-collaboration-results-of-a-survey-of-primary-care-in-quebec
#8
Kadija Perreault, Raynald Pineault, Roxane Borgès Da Silva, Sylvie Provost, Debbie E Feldman
OBJECTIVE: To assess the degree of collaboration in primary health care organizations between FPs and other health care professionals; and to identify organizational factors associated with such collaboration. DESIGN: Cross-sectional survey. SETTING: Primary health care organizations in the Montreal and Monteregie regions of Quebec. PARTICIPANTS: Physicians or administrative managers from 376 organizations. MAIN OUTCOME MEASURES: Degree of collaboration between FPs and other specialists and between FPs and nonphysician health professionals...
September 2017: Canadian Family Physician Médecin de Famille Canadien
https://www.readbyqxmd.com/read/28898419/a-block-matching-based-approach-with-multiple-simultaneous-templates-for-the-real-time-2d-ultrasound-tracking-of-liver-vessels
#9
Andrew J Shepard, Bo Wang, Thomas K F Foo, Bryan P Bednarz
PURPOSE: The implementation of motion management techniques in radiation therapy can aid in mitigating uncertainties and reducing margins. For motion management to be effective, it is necessary to track key structures both accurately and at a real-time speed. Therefore, the focus of this work was to develop a 2D algorithm for the real-time tracking of ultrasound features to aid in radiation therapy motion management. MATERIALS AND METHODS: The developed algorithm utilized a similarity measure-based block matching algorithm incorporating training methods and multiple simultaneous templates...
September 12, 2017: Medical Physics
https://www.readbyqxmd.com/read/28881251/registration-and-fusion-quantification-of-augmented-reality-based-nasal-endoscopic-surgery
#10
Yakui Chu, Jian Yang, Shaodong Ma, Danni Ai, Wenjie Li, Hong Song, Liang Li, Duanduan Chen, Lei Chen, Yongtian Wang
This paper quantifies the registration and fusion display errors of augmented reality-based nasal endoscopic surgery (ARNES). We comparatively investigated the spatial calibration process for front-end endoscopy and redefined the accuracy level of a calibrated endoscope by using a calibration tool with improved structural reliability. We also studied how registration accuracy was combined with the number and distribution of the deployed fiducial points (FPs) for positioning and the measured registration time...
August 3, 2017: Medical Image Analysis
https://www.readbyqxmd.com/read/28880156/a-high-resolution-mini-microscope-system-for-wireless-real-time-monitoring
#11
Zongjie Wang, Akash Boddeda, Benjamin Parker, Roya Samanipour, Sanjoy Ghosh, Frederic Menard, Keekyoung Kim
OBJECTIVE: Compact, cost-effective and high-performance microscope that enables the real-time imaging of cells and lab-on-a-chip devices is highly demanded for cell biology and biomedical engineering. This paper aims to present the design and application of an inexpensive wireless mini-microscope with resolution up to 2592 × 1944 pixels and speed up to 90 fps. METHODS: The mini-microscope system was built on a commercial embedded system (Raspberry Pi). We modified a camera module and adopted an inverse dual lens system to obtain the clear field of view and appropriate magnification for tens of micrometer objects...
September 4, 2017: IEEE Transactions on Bio-medical Engineering
https://www.readbyqxmd.com/read/28873733/bioaccessibility-and-antioxidant-activity-of-phenolic-compounds-in-frozen-pulps-of-brazilian-exotic-fruits-exposed-to-simulated-gastrointestinal-conditions
#12
Rodrigo Luiz Targino Dutra, Aline Macedo Dantas, Débora de Araújo Marques, Jéssica Dayanne Ferreira Batista, Bruno Raniere Lins de Albuquerque Meireles, Ângela Maria Tribuzy de Magalhães Cordeiro, Marciane Magnani, Graciele da Silva Campelo Borges
This study characterized the phenolic profile in siriguela, umbu-cajá and mangaba, as well in their frozen pulps (FPs). The bioaccessibility of phenolic compounds and the antioxidant activity of the FP dialysates were determined following exposure to simulated gastrointestinal conditions. The profile of phenolic compounds identified in fruit and FPs were relatively similar. After pulp processing, increases of 33.60% and 68.72% in free phenolics were observed for mangaba and umbu-cajá, respectively, whereas in siriguela, the free phenolic was reduced by 56...
October 2017: Food Research International
https://www.readbyqxmd.com/read/28867188/disruption-of-the-hydrogen-bonding-network-determines-the-ph-induced-non-fluorescent-state-of-the-fluorescent-protein-zsyellow-by-protonation-of-glu221
#13
Ji-Eun Bae, In Jung Kim, Ki Hyun Nam
Many fluorescent proteins (FPs) exhibit fluorescence quenching at a low pH. This pH-induced non-fluorescent state of an FP serves as a useful indicator of the cellular pH. ZsYellow is widely used as an optical marker in molecular biology, but its pH-induced non-fluorescent state has not been characterized. Here, we report the pH-dependent spectral properties of ZsYellow, which exhibited the pH-induced non-fluorescence state at a pH below 4.0. We determined the crystal structures of ZsYellow at pH 3.5 (non-fluorescence state) and 8...
August 31, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28867179/rage-maintains-high-levels-of-nf%C3%AE%C2%BAb-and-oncogenic-kras-activity-in-pancreatic-cancer
#14
Nancy Azizan, Melissa A Suter, Yan Liu, Craig D Logsdon
Oncogenic KRas activity is central to several cancer types including pancreatic ductal adenocarcinoma (PDAC) but has been determined to be "undruggable". Recent studies have indicated that oncogenic KRas is not constitutively active but relies on a feed-forward stimulatory mechanism involving NFκB mediated inflammation. In the current study, we investigated the role of the receptor for advanced glycation end-products (RAGE) in maintaining oncogenic signaling in PDAC. We observed that there was a shift in the levels of specific RAGE isoforms and altered cellular localization in PDAC...
August 31, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28863943/the-effect-of-testing-reliability-on-visual-field-sensitivity-in-normal-eyes-the-singapore-chinese-eye-study
#15
Nicholas Y Q Tan, Yih-Chung Tham, Victor Koh, Duc Quang Nguyen, Carol Y Cheung, Tin Aung, Tien Yin Wong, Ching-Yu Cheng
PURPOSE: To quantitatively determine how the reliability indices in standard automated perimetry (SAP) affect the global indices of visual field (VF) results in nonglaucomatous eyes. DESIGN: Observational, cross-sectional study. PARTICIPANTS: A total of 830 adults aged 40 to 80 years, without visual impairment, glaucoma, significant cataract, and major eye diseases, were selected from the population-based Singapore Chinese Eye Study (SCES)...
August 29, 2017: Ophthalmology
https://www.readbyqxmd.com/read/28863695/a-multi-frequency-electrical-impedance-tomography-system-for-real-time-2d-and-3d-imaging
#16
Yunjie Yang, Jiabin Jia
This paper presents the design and evaluation of a configurable, fast multi-frequency Electrical Impedance Tomography (mfEIT) system for real-time 2D and 3D imaging, particularly for biomedical imaging. The system integrates 32 electrode interfaces and the current frequency ranges from 10 kHz to 1 MHz. The system incorporates the following novel features. First, a fully adjustable multi-frequency current source with current monitoring function is designed. Second, a flexible switching scheme is developed for arbitrary sensing configuration and a semi-parallel data acquisition architecture is implemented for high-frame-rate data acquisition...
August 2017: Review of Scientific Instruments
https://www.readbyqxmd.com/read/28863200/autonomic-dysfunction-a-guide-for-fps
#17
Kristen Thornton, Marlon O Mitchell
Impotence, bladder dysfunction, GI symptoms, and orthostatic hypotension can signal autonomic dysfunction. Here's what you'll see and how to respond.
September 2017: Journal of Family Practice
https://www.readbyqxmd.com/read/28862719/kinematic-analysis-of-new-and-used-reciprocating-endodontic-motors-in-2-different-modes
#18
Özgür Irmak, Ekim O Orhan
PURPOSE: The actual reciprocating angles of endodontic motors might differ from the manufacturers' set values. This study analyzed the effect of clinical usage on the kinematics of reciprocating endodontic motors with 2 different reciprocal modes. METHODS: 1 new and 3 used reciprocating endodontic motors (X-Smart Plus, Dentsply Maillefer) with 2 different reciprocating modes, WaveOne mode (W-mode) or Reciproc mode (R-mode), were analyzed. An angle measurement disc was inserted into a contra-angle...
August 10, 2017: International Journal of Artificial Organs
https://www.readbyqxmd.com/read/28859297/amyloidogenic-medin-induces-endothelial-dysfunction-and-vascular-inflammation-through-the-receptor-for-advanced-glycation-endproducts
#19
Raymond Q Migrino, Hannah A Davies, Seth Truran, Nina Karamanova, Daniel A Franco, Thomas G Beach, Geidy E Serrano, Danh Truong, Mehdi Nikkhah, Jillian Madine
Aims: Medin is a common amyloidogenic protein in humans that accumulates in arteries with advanced age and has been implicated in vascular degeneration. Medin's effect on endothelial function remains unknown. The aims are to assess medin's effects on human arteriole endothelial function and identify potential mechanisms underlying medin-induced vascular injury. Methods and results: Ex vivo human adipose and leptomeningeal arterioles were exposed (1 h) to medin (0...
September 1, 2017: Cardiovascular Research
https://www.readbyqxmd.com/read/28851864/improved-split-fluorescent-proteins-for-endogenous-protein-labeling
#20
Siyu Feng, Sayaka Sekine, Veronica Pessino, Han Li, Manuel D Leonetti, Bo Huang
Self-complementing split fluorescent proteins (FPs) have been widely used for protein labeling, visualization of subcellular protein localization, and detection of cell-cell contact. To expand this toolset, we have developed a screening strategy for the direct engineering of self-complementing split FPs. Via this strategy, we have generated a yellow-green split-mNeonGreen21-10/11 that improves the ratio of complemented signal to the background of FP1-10-expressing cells compared to the commonly used split GFP1-10/11; as well as a 10-fold brighter red-colored split-sfCherry21-10/11...
August 29, 2017: Nature Communications
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