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https://www.readbyqxmd.com/read/28819250/oblique-scanning-laser-microscopy-for-simultaneously-volumetric-structural-and-molecular-imaging-using-only-one-raster-scan
#1
Lei Zhang, Amalia Capilla, Weiye Song, Gustavo Mostoslavsky, Ji Yi
Multi-modal three dimensional (3D) optical imaging combining both structural sensitivity and molecular specificity is highly desirable in biomedical research. In this paper, we present a method termed oblique scanning laser microscopy (OSLM) to combine optical coherence tomography (OCT), for simultaneously volumetric structural and molecular imaging with cellular resolution in all three dimensions. Conventional 3D laser scanning fluorescence microscopy requires repeated optical sectioning to create z-stacks in depth...
August 17, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28816706/prognostic-factors-for-recurrence-after-fertility-preserving-surgery-in-patients-with-borderline-ovarian-tumors-a-systematic-review-and-meta-analysis-of-observational-studies
#2
Xiaobing Jiao, Jun Hu, Lirong Zhu
OBJECTIVE: The aim of this study was to find the unfavorable prognostic factors for recurrence after fertility-preserving surgery (FPS) in patients with borderline ovarian tumors (BOTs). METHODS: To perform a meta-analysis to compare the recurrence rates of BOT patients after FPS according to different prognostic factors, we searched PubMed, EMBASE, and Cochrane for observational studies. Odds ratios (ORs) with 95% confidence intervals (CIs) were calculated with a fixed-effects model...
August 14, 2017: International Journal of Gynecological Cancer
https://www.readbyqxmd.com/read/28814478/effect-of-patient-weight-on-first-pass-success-and-neuromuscular-blocking-agent-dosing-for-rapid-sequence-intubation-in-the-emergency-department
#3
Asad E Patanwala, John C Sakles
OBJECTIVES: The primary objective of this study was to determine the association between patient weight and first pass success (FPS) during rapid sequence intubation (RSI) in the ED. The secondary objective was to evaluate the association between patient weight and neuromuscular blocking agent (NMBA) dosing. METHODS: This was a retrospective cohort study conducted in a tertiary care academic ED. Consecutive adult patients who underwent RSI in the ED between January 2014 and June 2016 were included...
August 16, 2017: Emergency Medicine Journal: EMJ
https://www.readbyqxmd.com/read/28807959/referral-processes-and-wait-times-in-primary-care
#4
Ieva Neimanis, Kathryn Gaebel, Robert Dickson, Richard Levy, Cindy Goebel, Angelo Zizzo, Anne Woods, John Corsini
OBJECTIVE: To evaluate the response times to requests for consultations from FPs and the wait times for patient appointments. DESIGN: Mailed invitation to participate in a survey about non-FP specialist consultation requests from April 28 to May 9, 2014. SETTING: Hamilton, Ont. PARTICIPANTS: All active physicians with community practices from the Department of Family Medicine at St Joseph's Healthcare Hamilton and Hamilton Health Sciences...
August 2017: Canadian Family Physician Médecin de Famille Canadien
https://www.readbyqxmd.com/read/28806860/patient-caregiver-and-provider-perceptions-of-pain-and-pain-management-in-adolescents-and-young-adults-with-bleeding-disorders
#5
Angela Lambing, Cynthia D Nichols, James E Munn, Terry L Anderson, Bartholomew J Tortella, Michelle L Witkop
INTRODUCTION: Recurrent bleeding and associated pain are critical components in the management of bleeding disorders, yet scant data describe perceptions of pain in this patient population. OBJECTIVE: This study assessed perceptions of pain and pain management in adolescents and young adults (AYAs) with haemophilia or von Willebrand disease (VWD) to determine agreement/disagreement between patients, caregivers and health care providers. METHODS: Using an online questionnaire, AYA patients (N=89), their caregivers (N=77), and providers (N=54) reported on pain perception, pain treatment and pain control...
August 14, 2017: Haemophilia: the Official Journal of the World Federation of Hemophilia
https://www.readbyqxmd.com/read/28805545/rural-family-physicians-are-twice-as-likely-to-use-telehealth-as-urban-family-physicians
#6
Anuradha Jetty, Miranda A Moore, Megan Coffman, Stephen Petterson, Andrew Bazemore
BACKGROUND: Telehealth has the potential to reduce health inequities and improve health outcomes among rural populations through increased access to physicians, specialists, and reduced travel time for patients. INTRODUCTION: Although rural telehealth services have expanded in several specialized areas, little is known about the attitudes, beliefs, and uptake of telehealth use in rural American primary care. This study characterizes the differences between rural and urban family physicians (FPs), their perceptions of telehealth use, and barriers to further adoption...
August 14, 2017: Telemedicine Journal and E-health: the Official Journal of the American Telemedicine Association
https://www.readbyqxmd.com/read/28795318/a-unified-methodology-based-on-sparse-field-level-sets-and-boosting-algorithms-for-false-positives-reduction-in-lung-nodules-detection
#7
Soudeh Saien, Hamid Abrishami Moghaddam, Mohsen Fathian
PURPOSE: This work aims to develop a unified methodology for the false positives reduction in lung nodules computer-aided detection schemes. METHODS: The 3D region of each detected nodule candidate is first reconstructed using the sparse field method for accurately segmenting the objects. This technique enhances the level set modeling by restricting the computations to a narrow band near the evolving curve. Then, a set of 2D and 3D relevant features are extracted for each segmented candidate...
August 9, 2017: International Journal of Computer Assisted Radiology and Surgery
https://www.readbyqxmd.com/read/28792483/monocular-stereo-measurement-using-high-speed-catadioptric-tracking
#8
Shaopeng Hu, Yuji Matsumoto, Takeshi Takaki, Idaku Ishi
This paper presents a novel concept of real-time catadioptric stereo tracking using a single ultrafast mirror-drive pan-tilt active vision system that can simultaneously switch between hundreds of different views in a second. By accelerating video-shooting, computation, and actuation at the millisecond-granularity level for time-division multithreaded processing in ultrafast gaze control, the active vision system can function virtually as two or more tracking cameras with different views. It enables a single active vision system to act as virtual left and right pan-tilt cameras that can simultaneously shoot a pair of stereo images for the same object to be observed at arbitrary viewpoints by switching the direction of the mirrors of the active vision system frame by frame...
August 9, 2017: Sensors
https://www.readbyqxmd.com/read/28774623/control-of-aliphatic-halogenated-dbp-precursors-with-multiple-drinking-water-treatment-processes-formation-potential-and-integrated-toxicity
#9
Yimeng Zhang, Wenhai Chu, Dechang Yao, Daqiang Yin
The comprehensive control efficiency for the formation potentials (FPs) of a range of regulated and unregulated halogenated disinfection by-products (DBPs) (including carbonaceous DBPs (C-DBPs), nitrogenous DBPs (N-DBPs), and iodinated DBPs (I-DBPs)) with the multiple drinking water treatment processes, including pre-ozonation, conventional treatment (coagulation-sedimentation, pre-sand filtration), ozone-biological activated carbon (O3-BAC) advanced treatment, and post-sand filtration, was investigated. The potential toxic risks of DBPs by combing their FPs and toxicity values were also evaluated...
August 2017: Journal of Environmental Sciences (China)
https://www.readbyqxmd.com/read/28771184/bacterial-phytochromes-cyanobacteriochromes-and-allophycocyanins-as-a-source-of-near-infrared-fluorescent-probes
#10
REVIEW
Olena S Oliinyk, Konstantin G Chernov, Vladislav V Verkhusha
Bacterial photoreceptors absorb light energy and transform it into intracellular signals that regulate metabolism. Bacterial phytochrome photoreceptors (BphPs), some cyanobacteriochromes (CBCRs) and allophycocyanins (APCs) possess the near-infrared (NIR) absorbance spectra that make them promising molecular templates to design NIR fluorescent proteins (FPs) and biosensors for studies in mammalian cells and whole animals. Here, we review structures, photochemical properties and molecular functions of several families of bacterial photoreceptors...
August 3, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28753915/colorful-packages-encapsulation-of-fluorescent-proteins-in-complex-coacervate-core-micelles
#11
Antsje Nolles, Adrie H Westphal, J Mieke Kleijn, Willem J H van Berkel, Jan Willem Borst
Encapsulation of proteins can be beneficial for food and biomedical applications. To study their biophysical properties in complex coacervate core micelles (C3Ms), we previously encapsulated enhanced green fluorescent protein (EGFP) and its monomeric variant, mEGFP, with the cationic-neutral diblock copolymer poly(2-methyl-vinyl-pyridinium)n-b-poly(ethylene-oxide)m (P2MVPn-b-PEOm) as enveloping material. C3Ms with high packaging densities of fluorescent proteins (FPs) were obtained, resulting in a restricted orientational freedom of the protein molecules, influencing their structural and spectral properties...
July 19, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28743618/deep-architecture-neural-network-based-real-time-image-processing-for-image-guided-radiotherapy
#12
Shinichiro Mori
INTRODUCTION: To develop real-time image processing for image-guided radiotherapy, we evaluated several neural network models for use with different imaging modalities, including X-ray fluoroscopic image denoising. METHODS & MATERIALS: Setup images of prostate cancer patients were acquired with two oblique X-ray fluoroscopic units. Two types of residual network were designed: a convolutional autoencoder (rCAE) and a convolutional neural network (rCNN). We changed the convolutional kernel size and number of convolutional layers for both networks, and the number of pooling and upsampling layers for rCAE...
July 22, 2017: Physica Medica: PM
https://www.readbyqxmd.com/read/28741031/inhibition-of-serotonin-transporters-disrupts-the-enhancement-of-fear-memory-extinction-by-3-4-methylenedioxymethamphetamine-mdma
#13
Matthew B Young, Seth D Norrholm, Lara M Khoury, Tanja Jovanovic, Sheila A M Rauch, Collin M Reiff, Boadie W Dunlop, Barbara O Rothbaum, Leonard L Howell
RATIONALE: 3,4-Methylenedioxymethamphetamine (MDMA) persistently improves symptoms of post-traumatic stress disorder (PTSD) when combined with psychotherapy. Studies in rodents suggest that these effects can be attributed to enhancement of fear memory extinction. Therefore, MDMA may improve the effects of exposure-based therapy for PTSD, particularly in treatment-resistant patients. However, given MDMA's broad pharmacological profile, further investigation is warranted before moving to a complex clinical population...
July 24, 2017: Psychopharmacology
https://www.readbyqxmd.com/read/28736675/simulation-study-on-compressive-laminar-optical-tomography-for-cardiac-action-potential-propagation
#14
Takumi Harada, Naoki Tomii, Shota Manago, Etsuko Kobayashi, Ichiro Sakuma
To measure the activity of tissue at the microscopic level, laminar optical tomography (LOT), which is a microscopic form of diffuse optical tomography, has been developed. However, obtaining sufficient recording speed to determine rapidly changing dynamic activity remains major challenges. For a high frame rate of the reconstructed data, we here propose a new LOT method using compressed sensing theory, called compressive laminar optical tomography (CLOT), in which novel digital micromirror device-based illumination and data reduction in a single reconstruction are applied...
April 1, 2017: Biomedical Optics Express
https://www.readbyqxmd.com/read/28727705/activation-of-the-rage-stat3-pathway-in-the-dorsal-root-ganglion-contributes-to-the-persistent-pain-hypersensitivity-induced-by-lumbar-disc-herniation
#15
Xin-Sheng Zhang, Xiao Li, Hai-Jie Luo, Zhu-Xi Huang, Cui-Cui Liu, Qing Wan, Shu-Wei Xu, Shao-Ling Wu, Song-Jian Ke, Chao Ma
BACKGROUND: Clinically, chronic low back pain and sciatica associated with lumbar disc herniation (LDH) is a common musculoskeletal disorder. Due to the unawareness of detailed mechanisms, it is difficult to get an effective therapy. OBJECTIVE: The aim of the present study was to identify the role of the RAGE/STAT3 pathway in the dorsal root ganglion (DRG) on the formation and development of persistent pain hypersensitivity induced by LDH. STUDY DESIGN: Controlled animal study...
July 2017: Pain Physician
https://www.readbyqxmd.com/read/28722771/navigating-and-negotiating-information-and-support-experiences-of-first-time-mothers
#16
Megan Aston, Sheri Price, Joelle Monaghan, Meaghan Sim, Andrea Hunter, Victoria Little
AIM: To examine how first time mothers in Nova Scotia (NS) identified and prioritized their own postpartum needs and where they went for information and support, inclusive of informal or formal programs and services, social media, family and friends. BACKGROUND: The early postpartum period is an exciting, yet stressful life experience for first time mothers. Mothers have often turned to families, friends and health care professionals for support and information; however, these social networks look differently today due to changes in institutional policies and postpartum health care practices, as well as the emergence of online resources and networks...
July 19, 2017: Journal of Clinical Nursing
https://www.readbyqxmd.com/read/28717570/chip-on-the-tip-compact-flexible-endoscopic-epifluorescence-video-microscope-for-in-vivo-imaging-in-medicine-and-biomedical-research
#17
Gregor Matz, Bernhard Messerschmidt, Werner Göbel, Severin Filser, Christian S Betz, Matthias Kirsch, Ortrud Uckermann, Marcel Kunze, Sven Flämig, André Ehrhardt, Klaus-Martin Irion, Mareike Haack, Mario M Dorostkar, Jochen Herms, Herbert Gross
We demonstrate a 60 mg light video-endomicroscope with a cylindrical shape of the rigid tip of only 1.6 mm diameter and 6.7 mm length. A novel implementation method of the illumination unit in the endomicroscope is presented. It allows for the illumination of the biological sample with fiber-coupled LED light at 455 nm and the imaging of the red-shifted fluorescence light above 500 nm in epi-direction. A large numerical aperture of 0.7 leads to a sub-cellular resolution and yields to high-contrast images within a field of view of 160 μm...
July 1, 2017: Biomedical Optics Express
https://www.readbyqxmd.com/read/28709324/geometric-structure-and-information-change-in-phase-transitions
#18
Eun-Jin Kim, Rainer Hollerbach
We propose a toy model for a cyclic order-disorder transition and introduce a geometric methodology to understand stochastic processes involved in transitions. Specifically, our model consists of a pair of forward and backward processes (FPs and BPs) for the emergence and disappearance of a structure in a stochastic environment. We calculate time-dependent probability density functions (PDFs) and the information length L, which is the total number of different states that a system undergoes during the transition...
June 2017: Physical Review. E
https://www.readbyqxmd.com/read/28704934/functioning-of-fluorescent-proteins-in-aggregates-in-anthozoa-species-and-in-recombinant-artificial-models
#19
Natalia V Povarova, Natalia D Petri, Anna E Blokhina, Alexey M Bogdanov, Nadya G Gurskaya, Konstantin A Lukyanov
Despite great advances in practical applications of fluorescent proteins (FPs), their natural function is poorly understood. FPs display complex spatio-temporal expression patterns in living Anthozoa coral polyps. Here we applied confocal microscopy, specifically, the fluorescence recovery after photobleaching (FRAP) technique to analyze intracellular localization and mobility of endogenous FPs in live tissues. We observed three distinct types of protein distributions in living tissues. One type of distribution, characteristic for Anemonia, Discosoma and Zoanthus, is free, highly mobile cytoplasmic localization...
July 12, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28703332/validation-of-biplane-high-speed-fluoroscopy-combined-with-two-different-non-invasive-tracking-methodologies-for-measuring-in-vivo-distal-limb-kinematics-of-the-horse
#20
S M Geiger, E Reich, P Böttcher, S Grund, J Hagen
BACKGROUND: Biplane high-speed fluoroscopy is a new method for gait analysis of the equine distal extremity. This is the first study validating the non-invasive tracking possibilities (Autoscoping and Scientific Rotoscoping) taking equine anatomy into account. OBJECTIVES: To determine the resolution with which Autoscoping and Scientific Rotoscoping depict motion of the equine phalanges in comparison to the invasive gold standard marker-based registration. STUDY DESIGN: Comparative ex vivo study...
July 13, 2017: Equine Veterinary Journal
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