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https://www.readbyqxmd.com/read/28717570/chip-on-the-tip-compact-flexible-endoscopic-epifluorescence-video-microscope-for-in-vivo-imaging-in-medicine-and-biomedical-research
#1
Gregor Matz, Bernhard Messerschmidt, Werner Göbel, Severin Filser, Christian S Betz, Matthias Kirsch, Ortrud Uckermann, Marcel Kunze, Sven Flämig, André Ehrhardt, Klaus-Martin Irion, Mareike Haack, Mario M Dorostkar, Jochen Herms, Herbert Gross
We demonstrate a 60 mg light video-endomicroscope with a cylindrical shape of the rigid tip of only 1.6 mm diameter and 6.7 mm length. A novel implementation method of the illumination unit in the endomicroscope is presented. It allows for the illumination of the biological sample with fiber-coupled LED light at 455 nm and the imaging of the red-shifted fluorescence light above 500 nm in epi-direction. A large numerical aperture of 0.7 leads to a sub-cellular resolution and yields to high-contrast images within a field of view of 160 μm...
July 1, 2017: Biomedical Optics Express
https://www.readbyqxmd.com/read/28709324/geometric-structure-and-information-change-in-phase-transitions
#2
Eun-Jin Kim, Rainer Hollerbach
We propose a toy model for a cyclic order-disorder transition and introduce a geometric methodology to understand stochastic processes involved in transitions. Specifically, our model consists of a pair of forward and backward processes (FPs and BPs) for the emergence and disappearance of a structure in a stochastic environment. We calculate time-dependent probability density functions (PDFs) and the information length L, which is the total number of different states that a system undergoes during the transition...
June 2017: Physical Review. E
https://www.readbyqxmd.com/read/28704934/functioning-of-fluorescent-proteins-in-aggregates-in-anthozoa-species-and-in-recombinant-artificial-models
#3
Natalia V Povarova, Natalia D Petri, Anna E Blokhina, Alexey M Bogdanov, Nadya G Gurskaya, Konstantin A Lukyanov
Despite great advances in practical applications of fluorescent proteins (FPs), their natural function is poorly understood. FPs display complex spatio-temporal expression patterns in living Anthozoa coral polyps. Here we applied confocal microscopy, specifically, the fluorescence recovery after photobleaching (FRAP) technique to analyze intracellular localization and mobility of endogenous FPs in live tissues. We observed three distinct types of protein distributions in living tissues. One type of distribution, characteristic for Anemonia, Discosoma and Zoanthus, is free, highly mobile cytoplasmic localization...
July 12, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28703332/validation-of-biplane-high-speed-fluoroscopy-combined-with-two-different-non-invasive-tracking-methodologies-for-measuring-in-vivo-distal-limb-kinematics-of-the-horse
#4
S M Geiger, E Reich, P Böttcher, S Grund, J Hagen
BACKGROUND: Biplane high-speed fluoroscopy is a new method for gait analysis of the equine distal extremity. This is the first study validating the non-invasive tracking possibilities (Autoscoping and Scientific Rotoscoping) taking equine anatomy into account. OBJECTIVES: To determine the resolution with which Autoscoping and Scientific Rotoscoping depict motion of the equine phalanges in comparison to the invasive gold standard marker-based registration. STUDY DESIGN: Comparative ex vivo study...
July 13, 2017: Equine Veterinary Journal
https://www.readbyqxmd.com/read/28701915/web-camera-based-eye-tracking-to-assess-visual-memory-on-a-visual-paired-comparison-task
#5
Nicholas T Bott, Alex Lange, Dorene Rentz, Elizabeth Buffalo, Paul Clopton, Stuart Zola
Background: Web cameras are increasingly part of the standard hardware of most smart devices. Eye movements can often provide a noninvasive "window on the brain," and the recording of eye movements using web cameras is a burgeoning area of research. Objective: This study investigated a novel methodology for administering a visual paired comparison (VPC) decisional task using a web camera.To further assess this method, we examined the correlation between a standard eye-tracking camera automated scoring procedure [obtaining images at 60 frames per second (FPS)] and a manually scored procedure using a built-in laptop web camera (obtaining images at 3 FPS)...
2017: Frontiers in Neuroscience
https://www.readbyqxmd.com/read/28701459/staff-perceptions-of-community-health-centre-team-function-in-ontario
#6
Jennifer Rayner, Laura Muldoon
OBJECTIVE: To examine perceptions of different staff groups about team functioning in mature, community-governed, interprofessional primary health care practices. DESIGN: Cross-sectional online survey. SETTING: The 75 community health centres (CHCs) in Ontario at the time of the study, which have cared for people with barriers to access to traditional health services in community-governed, interprofessional settings, providing medical, social, and community services since the 1970s...
July 2017: Canadian Family Physician Médecin de Famille Canadien
https://www.readbyqxmd.com/read/28690368/on-the-impact-of-model-selection-on-predictor-identification-and-parameter-inference
#7
Ruth M Pfeiffer, Andrew Redd, Raymond J Carroll
We assessed the ability of several penalized regression methods for linear and logistic models to identify outcome-associated predictors and the impact of predictor selection on parameter inference for practical sample sizes. We studied effect estimates obtained directly from penalized methods (Algorithm 1), or by refitting selected predictors with standard regression (Algorithm 2). For linear models, penalized linear regression, elastic net, smoothly clipped absolute deviation (SCAD), least angle regression and LASSO had a low false negative (FN) predictor selection rates but false positive (FP) rates above 20 % for all sample and effect sizes...
2017: Computational Statistics
https://www.readbyqxmd.com/read/28683465/abca3-phenotype-in-non-small-cell-lung-cancer-indicates-poor-outcome
#8
Tobias R Overbeck, Johanna Arnemann, Regina Waldmann-Beushausen, Lorenz Trümper, Friedrich A Schöndube, Kirsten Reuter-Jessen, Bernhard C Danner
BACKGROUND: ATP-binding cassette transport protein A3 (ABCA3) is expressed in non-small cell lung cancer (NSCLC). We hypothesize that high-level ABCA3 expression may have a negative prognostic impact in patients with NSCLC. METHODS: In 89 patients with NSCLC and curative intended surgery, we analyzed postoperative immunohistochemistry staining of primary tumors (anti-ABCA3) and clinicopathological parameters. We used a unidimensional four point score (FPS) system for intensity assessment and, furthermore, a combined bidimensional scoring of intensity and quantity resulting in the positive index (PI)...
July 6, 2017: Oncology
https://www.readbyqxmd.com/read/28664403/rage-specific-inhibitor-fps-zm1-attenuates-ages-induced-neuroinflammation-and-oxidative-stress-in-rat-primary-microglia
#9
Chao Shen, Yingjuan Ma, Ziling Zeng, Qingqing Yin, Yan Hong, Xunyao Hou, Xueping Liu
Advanced glycation end products (AGEs) enhance microglial activation and intensify the inflammatory response and oxidative stress in the brain. This process may occur due to direct cytotoxicity or interacting with AGEs receptors (RAGE), which are expressed on the surface of microglia. FPS-ZM1 is a high-affinity but nontoxic RAGE-specific inhibitor that has been recently shown to attenuate the Aβ-induced inflammatory response by blocking the ligation of Aβ to RAGE. In this study, we further investigated the effect of FPS-ZM1 on the AGEs/RAGE interaction and downstream elevation of neuroinflammation and oxidative stress in primary microglia cells...
June 29, 2017: Neurochemical Research
https://www.readbyqxmd.com/read/28663636/comparison-of-a-new-auto-controlled-injection-system-with-traditional-syringe-for-mandibular-infiltrations-in-children-a-randomized-clinical-trial
#10
Vemula Deepak, Ramasubba Reddy Challa, Rekhalakshmi Kamatham, Sivakumar Nuvvula
BACKGROUND: Pain in the dental operatory can have a profound effect on the behavior of children. AIM: The aim of this study is to evaluate the pain perception while administering local infiltration, in children undergoing dental extractions, using a new auto-controlled injection system. MATERIALS AND METHODS: Children in the age range of 6-10 years with teeth indicated for extraction were recruited and allocated to either Group I, computer-controlled injection system (auto system with special cartridge and compatible disposable 30-gauge, 10 mm needles), or Group II, traditional system (30-gauge, 10 mm needle and disposable traditional syringe)...
April 2017: Anesthesia, Essays and Researches
https://www.readbyqxmd.com/read/28661886/family-physician-access-to-specialist-advice-by-telephone-reduction-in-unnecessary-specialist-consultations-and-emergency-department-visits
#11
Margot Wilson, Garey Mazowita, Andrew Ignaszewski, Adeera Levin, Clay Barber, David Thompson, Sandra Barr, Scott Lear, Robert D Levy
PROBLEM ADDRESSED: Timely access to specialist care is an important issue for patients with mild to moderate symptoms, and wait times for referrals are currently quite long. OBJECTIVE OF PROGRAM: To provide FPs with quick telephone access to other specialists for treatment advice for patients with nonserious conditions that they would otherwise refer to specialist care. PROGRAM DESCRIPTION: The RACE (Rapid Access to Consultative Expertise) program is a telephone hot-line providing FPs and nurse practitioners in the Vancouver, BC, area with timely access to specialist consultations...
November 2016: Canadian Family Physician Médecin de Famille Canadien
https://www.readbyqxmd.com/read/28658960/the-influence-of-game-genre-on-internet-gaming-disorder
#12
Euihyeon Na, Inyoung Choi, Taek-Ho Lee, Hyeseon Lee, Mi Jung Rho, Hyun Cho, Dong Jin Jung, Dai-Jin Kim
Background and aims Although Internet gaming disorder (IGD) has been investigated in detail, minimal research has been conducted regarding the influence of different game genres on IGD. The aim of this study is to compare the characteristics of members of game genre-specific groups with IGD and to identify factors associated with IGD status in each group in a large sample of adults. Methods Internet games were categorized into four genres: real-time strategy games, massive multiplayer online role-playing games (MMORPG), sport games, and first-person shooter (FPS) games...
June 29, 2017: Journal of Behavioral Addictions
https://www.readbyqxmd.com/read/28658810/quality-assessment-of-family-planning-sterilization-services-at-health-care-facilities-case-record-audit
#13
Medha Mathur, Ram Chandra Goyal, Navgeet Mathur
INTRODUCTION: Quality of sterilization services is a matter of concern in India because population control is a necessity. Family Planning Sterilization (FPS) services provided at public health care facilities need to be as per Standard Operating Procedures. AIM: To assess the quality of FPS services by audit of case records at selected health care facilities. MATERIALS AND METHODS: This cross-sectional study was conducted for two and a half year duration at selected public health care facilities of central India by simple random sampling where FPS services were provided...
May 2017: Journal of Clinical and Diagnostic Research: JCDR
https://www.readbyqxmd.com/read/28650801/300-fps-salient-object-detection-via-minimum-directional-contrast
#14
Xiaoming Huang, Yu-Jin Zhang
Global contrast considers the color difference between a target region or pixel and the rest of the image. It is frequently used to measure the saliency of the region or pixel. In previous global contrast-based methods, saliency is usually measured by the sum of contrast from the entire image. We find that the spatial distribution of contrast is one important cue of saliency that is neglected by previous works. Foreground pixel usually has high contrast from all directions, since it is surrounded by the background...
September 2017: IEEE Transactions on Image Processing: a Publication of the IEEE Signal Processing Society
https://www.readbyqxmd.com/read/28645008/effect-of-microrna-129-5p-targeting-hmgb1-rage-signaling-pathway-on-revascularization-in-a-collagenase-induced-intracerebral-hemorrhage-rat-model
#15
Xin-Long Ma, Shu-Ya Li, Feng Shang
BACKGROUND: This study aimed at exploring the effect of microRNA-129-5p (miR-129-5p) targeting high mobility group box-1 (HMGB1)-receptor for advanced glycation end-products (RAGE) signaling pathway on the revascularization in a collagenase-induced intracerebral hemorrhage (ICH) rat model. METHODS: OX26-pGFAP-IL, an immunoliposome expressing miR-129-5p was constructed. The collagenase-induced ICH rat models were successfully established by 96 Sprague Dawley (SD) rats, which were categorized into the sham group, ICH group, miR-129-5p group, negative control (NC) group, ethyl pyruvate (EP, an inhibitor of HMGB1) group and N-benzyl-4-chloro-N-cyclohe-xylbenzamide (FPS-ZM1, a RAGE receptor antagonist) group...
June 20, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/28643093/the-impact-of-trained-radiographers-as-concurrent-readers-on-performance-and-reading-time-of-experienced-radiologists-in-the-uk-lung-cancer-screening-ukls-trial
#16
Arjun Nair, Nicholas J Screaton, John A Holemans, Diane Jones, Leigh Clements, Bruce Barton, Natalie Gartland, Stephen W Duffy, David R Baldwin, John K Field, David M Hansell, Anand Devaraj
OBJECTIVES: To compare radiologists' performance reading CTs independently with their performance using radiographers as concurrent readers in lung cancer screening. METHODS: 369 consecutive baseline CTs performed for the UK Lung Cancer Screening (UKLS) trial were double-read by radiologists reading either independently or concurrently with a radiographer. In concurrent reading, the radiologist reviewed radiographer-identified nodules and then detected any additional nodules...
June 22, 2017: European Radiology
https://www.readbyqxmd.com/read/28638406/an-html5-based-pure-website-solution-for-rapidly-viewing-and-processing-large-scale-3d-medical-volume-reconstruction-on-mobile-internet
#17
Liang Qiao, Xin Chen, Ye Zhang, Jingna Zhang, Yi Wu, Ying Li, Xuemei Mo, Wei Chen, Bing Xie, Mingguo Qiu
This study aimed to propose a pure web-based solution to serve users to access large-scale 3D medical volume anywhere with good user experience and complete details. A novel solution of the Master-Slave interaction mode was proposed, which absorbed advantages of remote volume rendering and surface rendering. On server side, we designed a message-responding mechanism to listen to interactive requests from clients (Slave model) and to guide Master volume rendering. On client side, we used HTML5 to normalize user-interactive behaviors on Slave model and enhance the accuracy of behavior request and user-friendly experience...
2017: International Journal of Telemedicine and Applications
https://www.readbyqxmd.com/read/28637215/managerial-ownership-in-nursing-homes-staffing-quality-and-financial-performance
#18
Sean Shenghsiu Huang, John R Bowblis
Purpose of the Study: Ownership of nursing homes (NHs) has primarily focused broadly on differences between for-profit (FP), nonprofit (NFP), and government-operated facilities. Yet, among FPs, the understanding of detailed ownership structures at individual NHs is rather limited. Particularly, NH administrators may hold significant equity interests in their facilities, leading to heterogeneous financial incentives and NH outcomes. Through the principal-agent theory, this article studies how managerial ownership of individual facilities affects NH outcomes...
June 20, 2017: Gerontologist
https://www.readbyqxmd.com/read/28636361/machine-learning-assisted-approach-for-discovering-novel-inhibitors-targeting-bromodomain-containing-protein-4
#19
Jing Xing, Wenchao Lu, Rongfeng Liu, Yulan Wang, Yiqian Xie, Hao Zhang, Zhe Shi, Hao Jiang, Yu-Chih Liu, Kaixian Chen, Hualiang Jiang, Cheng Luo, Mingyue Zheng
Bromodomain-containing protein 4 (BRD4) is implicated in the pathogenesis of a number of different cancers, inflammatory diseases and heart failure. Much effort has been dedicated toward discovering novel scaffold BRD4 inhibitors (BRD4is) with different selectivity profiles and potential antiresistance properties. Structure-based drug design (SBDD) and virtual screening (VS) are the most frequently used approaches. Here, we demonstrate a novel, structure-based VS approach that uses machine-learning algorithms trained on the priori structure and activity knowledge to predict the likelihood that a compound is a BRD4i based on its binding pattern with BRD4...
July 10, 2017: Journal of Chemical Information and Modeling
https://www.readbyqxmd.com/read/28627330/gpu-accelerated-kernel-regression-reconstruction-for-freehand-3d-ultrasound-imaging
#20
Tiexiang Wen, Ling Li, Qingsong Zhu, Wenjian Qin, Jia Gu, Feng Yang, Yaoqin Xie
Volume reconstruction method plays an important role in improving reconstructed volumetric image quality for freehand three-dimensional (3D) ultrasound imaging. By utilizing the capability of programmable graphics processing unit (GPU), we can achieve a real-time incremental volume reconstruction at a speed of 25-50 frames per second (fps). After incremental reconstruction and visualization, hole-filling is performed on GPU to fill remaining empty voxels. However, traditional pixel nearest neighbor-based hole-filling fails to reconstruct volume with high image quality...
July 2017: Ultrasonic Imaging
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