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https://www.readbyqxmd.com/read/29791788/shift-in-skin-microbiota-of-western-european-women-across-aging
#1
Romain Jugé, Pauline Rouaud-Tinguely, Josselin Breugnot, Katia Servaes, Christine Grimaldi, Marie-Paule Roth, Hélène Coppin, Brigitte Closs
AIMS: The objective of our study was to compare the microbiota diversity between two different age groups of Western European women. METHODS AND RESULTS: Skin-swab samples were collected directly on the forehead of 34 healthy Western European women: 17 younger (21-31 years old) and 17 older individuals (54-69 years old). Bacterial communities were evaluated using the 16S rRNA gene sequencing. Data revealed a higher alpha-diversity on the skin of older individuals compared to younger ones...
May 23, 2018: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/29786766/bacterial-dna-detected-on-pathologically-changed-heart-valves-using-16s-rrna-gene-amplification
#2
Miroslava Chalupova, Anna Skalova, Tomas Hajek, Lenka Geigerova, Dana Kralova, Pavel Liska, Hana Hecova, Jiri Molacek, Jaroslav Hrabak
Nowadays, dental diseases are one of the most common illnesses in the world. Some of them can lead to translocation of oral bacteria to the bloodstream causing intermittent bacteraemia. Therefore, a potential association between oral infection and cardiovascular diseases has been discussed in recent years as a result of adhesion of oral microbes to the heart valves. The aim of this study was to detect oral bacteria on pathologically changed heart valves not caused by infective endocarditis. In the study, patients with pathologically changed heart valves were involved...
May 22, 2018: Folia Microbiologica
https://www.readbyqxmd.com/read/29784985/chronic-rhinosinusitis-with-nasal-polyps-is-characterized-by-dysbacteriosis-of-the-nasal-microbiota
#3
Thanit Chalermwatanachai, Ramiro Vilchez-Vargas, Gabriele Holtappels, Tim Lacoere, Ruy Jáuregui, Frederiek-Maarten Kerckhof, Dietmar H Pieper, Tom Van de Wiele, Mario Vaneechoutte, Thibaut Van Zele, Claus Bachert
Chronic rhinosinusitis with nasal polyp (CRSwNP) patients are often characterized by asthma comorbidity and a type-2 inflammation of the sinonasal mucosa. The mucosal microbiota has been suggested to be implicated in the persistence of inflammation, but associations have not been well defined. To compare the bacterial communities of healthy subjects with CRSwNP patients, we collected nasal swabs from 17 healthy subjects, 21 CRSwNP patients without asthma (CRSwNP-A), and 20 CRSwNP patients with co-morbid asthma (CRSwNP+A)...
May 21, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29782471/new-approach-in-acne-therapy-a-specific-bacteriocin-activity-and-a-targeted-anti-il-8-property-in-just-1-probiotic-strain-the-l-salivarius-ls03
#4
Francesca Deidda, Angela Amoruso, Stefania Nicola, Teresa Graziano, Marco Pane, Luca Mogna
GOALS: The aim of this research was to assess the antibacterial activity of Lactobacillus salivarius LS03 (DSM 22776) against Propionibacterium acnes and its anti-inflammatory properties by inhibiting P. acnes-induced interleukin-8 (IL-8) release. BACKGROUND: Acne is the most common skin disease, causing significant psychosocial problems for those afflicted. Currently available agents for acne treatment, such as oral antibiotics, have limited use. Thus, development of novel agents to treat this disease is needed...
May 18, 2018: Journal of Clinical Gastroenterology
https://www.readbyqxmd.com/read/29780369/identification-and-removal-of-contaminant-sequences-from-ribosomal-gene-databases-lessons-from-the-census-of-deep-life
#5
Cody S Sheik, Brandi Kiel Reese, Katrina I Twing, Jason B Sylvan, Sharon L Grim, Matthew O Schrenk, Mitchell L Sogin, Frederick S Colwell
Earth's subsurface environment is one of the largest, yet least studied, biomes on Earth, and many questions remain regarding what microorganisms are indigenous to the subsurface. Through the activity of the Census of Deep Life (CoDL) and the Deep Carbon Observatory, an open access 16S ribosomal RNA gene sequence database from diverse subsurface environments has been compiled. However, due to low quantities of biomass in the deep subsurface, the potential for incorporation of contaminants from reagents used during sample collection, processing, and/or sequencing is high...
2018: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29771360/patient-and-treatment-characteristics-by-infecting-organism-in-cerebrospinal-fluid-shunt-infection
#6
Tamara D Simon, Matthew P Kronman, Kathryn B Whitlock, Samuel R Browd, Richard Holubkov, John R W Kestle, Abhaya V Kulkarni, Marcie Langley, David D Limbrick, Thomas G Luerssen, Jerry Oakes, Jay Riva-Cambrin, Curtis Rozzelle, Chevis N Shannon, Mandeep Tamber, John C Wellons Iii, William E Whitehead, Nicole Mayer-Hamblett
Background: Previous studies of cerebrospinal fluid (CSF) shunt infection treatment have been limited in size and unable to compare patient and treatment characteristics by infecting organism. Our objective was to describe variation in patient and treatment characteristics for children with first CSF shunt infection, stratified by infecting organism subgroups outlined in the 2017 Infectious Disease Society of America's (IDSA) guidelines. Methods: We studied a prospective cohort of children <18 years of age undergoing treatment for first CSF shunt infection at one of 7 Hydrocephalus Clinical Research Network hospitals from April 2008 to December 2012...
May 15, 2018: Journal of the Pediatric Infectious Diseases Society
https://www.readbyqxmd.com/read/29755418/microbiome-and-culture-based-analysis-of-chronic-rhinosinusitis-compared-to-healthy-sinus-mucosa
#7
Kerstin Koeller, Daniel P R Herlemann, Tobias Schuldt, Attila Ovari, Ellen Guder, Andreas Podbielski, Bernd Kreikemeyer, Bernhard Olzowy
The role of bacteria in chronic rhinosinusitis (CRS) is still not well understood. Whole microbiome analysis adds new aspects to our current understanding that is mainly based on isolated bacteria. It is still unclear how the results of microbiome analysis and the classical culture based approaches interrelate. To address this, middle meatus swabs and tissue samples were obtained during sinus surgery in 5 patients with CRS with nasal polyps (CRSwNP), 5 patients with diffuse CRS without nasal polyps (CRSsNP), 5 patients with unilateral purulent maxillary CRS (upm CRS) and 3 patients with healthy sinus mucosa...
2018: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29750587/the-microbiologic-profile-of-dacryocystitis
#8
Stella Y Chung, Leon Rafailov, Roger E Turbin, Paul D Langer
PURPOSE: Recent studies suggest an increasing incidence of gram-negative bacteria and methicillin-resistant Staphylococcus aureus in dacryocystitis. Since patients are often treated empirically without culture data, a changing microbiologic profile will markedly affect the success of oral treatment. To provide current guidelines for the treatment of this common condition, we investigated the microbiology and antibiogram of dacryocystitis seen at our institution. METHODS: The charts of all patients presenting with acute and/or chronic dacryocystitis in University Hospital, Newark, from 2007 to 2015 were reviewed...
May 11, 2018: Orbit
https://www.readbyqxmd.com/read/29741277/rational-design-and-synthesis-of-modified-teixobactin-analogues-in-vitro-antibacterial-activity-against-staphylococcus-aureus-propionibacterium-acnes-and-pseudomonas-aeruginosa
#9
Vivian Ng, Sarah A Kuehne, Weng Chan
Teixobactin, a recently discovered depsipeptide that binds to bacterial lipid II and lipid III, provides a promising molecular scaffold for the design of new antimicrobials. Herein, we describe the synthesis and antimicrobial evaluation of systematically modified teixobactin analogues. The replacement of Ile11 residue with aliphatic isosteres, the modification of the guanidino group at residue 10 and the introduction of a rigidifying residue, dehydroamino acid into the macrocyclic ring generated useful structure-activity information...
May 9, 2018: Chemistry: a European Journal
https://www.readbyqxmd.com/read/29733991/varicella-zoster-virus-infection-and-osteomyelitis-of-the-skull-a-case-report
#10
Taylor Sommer, Michael Karsy, Meghan J Driscoll, Randy L Jensen
BACKGROUND: Varicella zoster virus (VZV) is a common herpesvirus infection that can result in acute varicella/chickenpox as well as delayed activation in herpes zoster/shingles. Ramsay-Hunt syndrome is a rare presentation of VZV reactivation, involving 1% of cases and resulting in lesion formation along the 7th cranial nerve distribution. We report the first case of a patient who presented with acute calvarial osteomyelitis after VZV reactivation and Propionibacterium acnes suprainfection...
May 4, 2018: World Neurosurgery
https://www.readbyqxmd.com/read/29722425/comparative-study-of-the-bactericidal-effects-of-indocyanine-green-and-methyl-aminolevulinate-based-photodynamic-therapy-on-propionibacterium-acnes-as-a-new-treatment-for-acne
#11
Seung-Hwan Choi, Jeong-Wan Seo, Ki-Ho Kim
Acne vulgaris is one of the most common dermatological problems, and its therapeutic options include topical and systemic retinoids and antibiotics. However, increase in problems associated with acne treatment, such as side-effects from conventional agents and bacterial resistance to antibiotics, has led to greater use of photodynamic therapy. The purpose of this study was to compare the bactericidal effects of indocyanine green- and methyl aminolevulinate-based photodynamic therapy on Propionibacterium acnes...
May 3, 2018: Journal of Dermatology
https://www.readbyqxmd.com/read/29720593/nano-photothermal-ablation-effect-of-hydrophilic-and-hydrophobic-functionalized-gold-nanorods-on-staphylococcus-aureus-and-propionibacterium-acnes
#12
Nouf N Mahmoud, Alaaldin M Alkilany, Enam A Khalil, Amal G Al-Bakri
The potential photothermal bactericidal activity of hydrophilic functionalized poly ethylene glycol (PEG)-gold nanorods (GNR) and hydrophobic functionalized polystyrene (PS)-GNR was evaluated towards strains of Staphylococcus aureus (S. aureus) and Propionibacterium acnes (P. acnes) by measuring the percentage reduction of bacterial viable count upon GNR excitation with a near infra-red (NIR) laser beam. Our results suggest that functionalized GNR had a minimal bactericidal activity against S. aureus and P...
May 2, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29719771/bioremediation-of-oil-based-drill-cuttings-by-a-halophilic-consortium-isolated-from-oil-contaminated-saline-soil
#13
Maryam Rezaei Somee, Mahmoud Shavandi, Seyed Mohammad Mehdi Dastgheib, Mohammad Ali Amoozegar
Oil-based drill cuttings are hazardous wastes containing complex hydrocarbons, heavy metals, and brine. Their remediation is a crucial step before release to the environment. In this work, we enriched a halophilic consortium, from oil-polluted saline soil, which is capable of degrading diesel as the main pollutant of oil-based drill cuttings. The degradation ability of the consortium was evaluated in microcosms using two different diluting agents (fine sand and biologically active soil). During the bioremediation process, the bacterial community dynamics of the microcosms was surveyed using PCR amplification of a fragment of 16S rRNA gene followed by denaturing gradient gel electrophoresis (DGGE)...
May 2018: 3 Biotech
https://www.readbyqxmd.com/read/29707057/-propionibacterium-acnes-infections-in-patients-with-idiopathic-scoliosis-a-case-control-study-and-review-of-the-literature
#14
I Swarup, J Gruskay, M Price, J Yang, J Blanco, S Perlman, R Widmann
Purpose: Surgical site infection (SSI) caused by Propionibacterium acnes is an infrequent but devastating complication after spinal fusion. The purpose of this study was to identify risk factors for SSI with Propionibacterium acnes after spinal fusion for juvenile and adolescent idiopathic scoliosis (JIS and AIS). Methods: A case-control study was performed. Each case was matched 2:1 for age, gender and diagnosis. Retrospective chart review was performed to obtain relevant demographic, surgical and clinical data for all cases and controls...
April 1, 2018: Journal of Children's Orthopaedics
https://www.readbyqxmd.com/read/29704883/eps-production-by-propionibacterium-freudenreichii-facilitates-its-immobilization-for-propionic-acid-production
#15
Fabricio Dos Santos Belgrano, Brunno Renato Farias Verçoza, Juliany Cola Fernandes Rodrigues, Rajni Hatti-Kaul, Nei Pereira
AIMS: Immobilization of microbial cells is a useful strategy for developing high cell density bioreactors with improved stability and productivity for production of different chemicals. Functionalization of the immobilization matrix or biofilm forming property of some strains have been utilized for achieving cell attachment. The aim of the present study was to investigate the production of exopolysaccharide (EPS) by Propionibacterium freudenreichii C.I.P 59.32 and utilize this feature for immobilization of the cells on porous glass beads for production of propionic acid...
April 28, 2018: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/29696720/bacterial-biofilms-a-possible-mechanism-for-chronic-infection-in-patients-with-lumbar-disc-herniation-a-prospective-proof-of-concept-study-using-fluorescence-in-situ-hybridization
#16
Søren Ohrt-Nissen, Blaine G Fritz, Jonas Walbom, Kasper N Kragh, Thomas Bjarnsholt, Benny Dahl, Claus Manniche
A relationship has been suggested between lumbar disc herniation (LDH) and chronic bacterial infection frequently involving Propionibacterium acnes, which is known to cause chronic infection through the formation of biofilm aggregates. The objective of the study was to assess whether a disc infection involving biofilm formation is present in patients with LDH. A total of 51 LDH patients and 14 controls were included. Bacterial DNA was detected by real-time polymerase chain reaction (PCR) in 16/51 samples in the LDH group and 7/14 controls (p = 0...
May 2018: APMIS: Acta Pathologica, Microbiologica, et Immunologica Scandinavica
https://www.readbyqxmd.com/read/29687917/pseudopropionibacterium-sp-nov-a-novel-red-pigmented-species-isolated-from-human-gingival-sulcus
#17
Masanori Saito, Noriko Shinozaki-Kuwahara, Osamu Tsudukibashi, Tomomi Hashizume-Takizawa, Ryoki Kobayashi, Tomoko Kurita-Ochiai
Strain SK-1T is a novel Gram stain-positive, pleomorphic, rod-shaped, non-spore forming, and non-motile organism, designated SK-1T , isolated from human gingival sulcus that produces acetic acid, propionic acid, lactic acid, and succinic acid as end products of glucose fermentation. Strain SK-1T had the closest relatedness to Pseudopropionibacterium (Propionibacterium) propionicum with sequence homologies of the 16S rRNA and RNA polymerase β subunit (rpoB) genes of 96.6% and 93.1%, respectively. The genomic DNA G + C content of the isolate was 61...
April 24, 2018: Microbiology and Immunology
https://www.readbyqxmd.com/read/29685273/silage-review-recent-advances-and-future-uses-of-silage-additives
#18
REVIEW
R E Muck, E M G Nadeau, T A McAllister, F E Contreras-Govea, M C Santos, L Kung
Additives have been available for enhancing silage preservation for decades. This review covers research studies published since 2000 that have investigated the efficacy of silage additives. The review has been divided into 6 categories of additives: homofermentative lactic acid bacteria (LAB), obligate heterofermentative LAB, combination inoculants containing obligate heterofermentative LAB plus homofermentative LAB, other inoculants, chemicals, and enzymes. The homofermentative LAB rapidly decrease pH and increase lactic acid relative to other fermentation products, although a meta-analysis indicated no reduction in pH in corn, sorghum, and sugarcane silages relative to untreated silages...
May 2018: Journal of Dairy Science
https://www.readbyqxmd.com/read/29684425/delivery-of-adapalene-using-a-novel-topical-gel-based-on-tea-tree-oil-nano-emulsion-permeation-antibacterial-and-safety-assessments
#19
Roqya Najafi-Taher, Behnaz Ghaemi, Amir Amani
The aim of present study was to design and optimize 0.1% adapalene loaded nano-emulsion to improve the efficacy of the drug efficacy and increase its user compliance. Effect of type and concentration of surfactants was studied on size of 0.1% adapalene loaded nano-emulsion. Optimized formulation was then evaluated for particle size, polydispersity index, morphology, viscosity, and pH. Subsequently, 1% carbopol® 934 was incorporated to the optimized formulation for preparation of its gel form. The efficacy and safety of 0...
April 20, 2018: European Journal of Pharmaceutical Sciences
https://www.readbyqxmd.com/read/29676446/an-approach-on-the-potential-use-of-probiotics-in-the-treatment-of-skin-conditions-acne-and-atopic-dermatitis
#20
REVIEW
Vitória H M Mottin, Edna S Suyenaga
Acne and Atopic Dermatitis (AD) are chronic inflammatory skin conditions with severe impact on a patient's life. Current treatments are related to adverse effects and do not represent a definitive cure. The present paper reviews the alterations in skin microbiome, specifically in acne and AD, and aims in searching for potential treatments based on benefic microorganisms, called probiotics. The review was made through bibliographic search of the main databases (Science Direct, PubMed, Scielo, Medline) between September 2015 and June 2016...
April 20, 2018: International Journal of Dermatology
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