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https://www.readbyqxmd.com/read/28336770/ptac10-a-key-subunit-of-plastid-encoded-rna-polymerase-promotes-chloroplast-development
#1
Sun Hyun Chang, Sangyool Lee, Tae Young Um, Ju-Kon Kim, Yang Do Choi, Geupil Jang
Regulation of photosynthetic gene expression by plastid-encoded RNA polymerase (PEP) is essential for chloroplast development. The activity of PEP largely relies on at least 12 PEP-associated proteins (PAPs) encoded in the nuclear genome of plant cells. A recent model proposed that these PAPs regulate the establishment of the PEP complex through broad PAP-PEP or PAP-PAP interactions. In this study, we identified the Arabidopsis thaliana seedling lethal mutant ptac10-1, which has defects in chloroplast development, and found that the mutant phenotype is caused by the suppression of PLASTID S1 RNA-BINDING DOMAIN PROTEIN (pTAC10/PAP3)...
March 23, 2017: Plant Physiology
https://www.readbyqxmd.com/read/28319270/evaluation-of-a-transparent-perfluorodecalin-infused-patch-as-an-adjunct-to-laser-assisted-tattoo-removal-a-pivotal-trial
#2
Brian S Biesman, Cara Costner
BACKGROUND AND OBJECTIVE: Laser-assisted treatment of tattoos is well recognized to produce opaque epidermal whitening that prevents multiple sequential passes during a single treatment session. The amount of epidermal whitening produced in association with the procedure can be minimized by topical application of perfluorodecalin (PFD), which is an optical clearing agent. This pivotal trial assessed the ability of a transparent PFD-infused patch used in conjunction with a Q-switched nanosecond laser in the treatment of tattoos to permit multiple laser passes during a single 5 minute treatment session in comparison to the number of passes that could be completed using conventional treatment of the tattoo with the laser alone...
March 20, 2017: Lasers in Surgery and Medicine
https://www.readbyqxmd.com/read/28303471/effect-of-acidity-of-in-office-bleaching-gels-on-tooth-sensitivity-and-whitening-a-two-center-double-blind-randomized-clinical-trial
#3
A D Loguercio, F Servat, R Stanislawczuk, A Mena-Serrano, M Rezende, M V Prieto, V Cereño, M F Rojas, K Ortega, E Fernandez, A Reis
OBJECTIVES: The study aimed to compare the tooth sensitivity (TS) and bleaching efficacy of two hydrogen peroxide gels with different pHs (acid pH [Pola Office, SDI] and the neutral pH [Pola Office+, SDI]) used for in-office bleaching. MATERIALS AND METHODS: Fifty-four patients from Brazil and Chile, with right superior incisor darker than A2, were selected for this double-blind, split-mouth randomized trial. Teeth were bleached in two sessions, with 1-week interval...
March 17, 2017: Clinical Oral Investigations
https://www.readbyqxmd.com/read/28299326/production-of-human-cu-zn-sod-with-higher-activity-and-lower-toxicity-in-e-coli-via-mutation-of-free-cysteine-residues
#4
Kun Zhang, Yuejuan Zhang, Jing Zi, Xiaochang Xue, Yi Wan
Although, as an antioxidant enzyme, human Cu,Zn superoxide dismutase 1 (hSOD1) can mitigate damage to cell components caused by free radicals generated by aerobic metabolism, large-scale manufacturing and clinical use of hSOD1 are still limited by the challenge of rapid and inexpensive production of high-quality eukaryotic hSOD1 in recombinant forms. We have demonstrated previously that it is a promising strategy to increase the expression levels of soluble hSOD1 so as to increase hSOD1 yields in E. coli. In this study, a wild-type hSOD1 (wtSOD1) and three mutant SOD1s (mhSOD1s), in which free cysteines were substituted with serine, were constructed and their expression in soluble form was measured...
2017: BioMed Research International
https://www.readbyqxmd.com/read/28298992/effects-of-three-different-bleaching-agents-on-microhardness-and-roughness-of-composite-sample-surfaces-finished-with-different-polishing-techniques
#5
İhsan Yikilgan, Hanife Kamak, Sinem Akgul, Suat Ozcan, Oya Bala
BACKGROUND: The aim of this study was to evaluate effects of different polishing methods and whitening agents on surface hardness and roughness of nano-hybrid composite resin. MATERIAL AND METHODS: In total, one hundred twenty disc-shaped specimens were prepared to nano-hybrid composite (Charisma Diamond). 60 samples were used for microhardness measurements and the others were used for the evaluation of surface roughness. Samples were divided randomly into two subgroups (n = 30 each)...
March 2017: Journal of Clinical and Experimental Dentistry
https://www.readbyqxmd.com/read/28298972/effectiveness-of-sodium-bicarbonate-combined-with-hydrogen-peroxide-and-cpp-acpf-in-whitening-and-microhardness-of-enamel
#6
Farzaneh Ahrari, Nadia Hasanzadeh, Omid Rajabi, Zakiyeh Forouzannejad
BACKGROUND: This study investigated the effects of sodium bicarbonate (NaHCO3) combined with 1.5% hydrogen peroxide (H2O2) and casein phosphopeptide amorphous calcium phosphate fluoride (CPP-ACPF) on color and microhardness of enamel. MATERIAL AND METHODS: Seventy-five bovine incisors were immersed in a tea solution for 7.5 days. The specimens were randomly divided into five groups according to the whitening agent applied: 1) 94% NaHCO3, 2) a blend of 94% NaHCO3 and CPP-ACPF, 3) a blend of 94% NaHCO3 and 1...
March 2017: Journal of Clinical and Experimental Dentistry
https://www.readbyqxmd.com/read/28295030/plarmeb-integration-of-least-angle-regression-with-empirical-bayes-for-multilocus-genome-wide-association-studies
#7
J Zhang, J-Y Feng, Y-L Ni, Y-J Wen, Y Niu, C L Tamba, C Yue, Q Song, Y-M Zhang
Multilocus genome-wide association studies (GWAS) have become the state-of-the-art procedure to identify quantitative trait nucleotides (QTNs) associated with complex traits. However, implementation of multilocus model in GWAS is still difficult. In this study, we integrated least angle regression with empirical Bayes to perform multilocus GWAS under polygenic background control. We used an algorithm of model transformation that whitened the covariance matrix of the polygenic matrix K and environmental noise...
March 15, 2017: Heredity
https://www.readbyqxmd.com/read/28278663/melanogenesis-inhibitors-from-coix-lacryma-jobi-seeds-in-b16-f10-melanoma-cells
#8
Yhiya Amen, Enos Tangke Arung, Mohamed S Afifi, Ahmed F Halim, Ahmed Ashour, Ryoji Fujimoto, Takahiro Goto, Kuniyoshi Shimizu
An adenine derivative, 9-β-D-glucopyranosyl adenine, reported for the first time from a natural source, in addition to nine known compounds were isolated from the seeds of Coix lacryma-jobi. Their structures were elucidated based on extensive spectroscopic and chemical studies. The isolated compounds and the ethanol extract have been assayed for melanin inhibition using B16-F10 melanoma cell line. The results of our study suggested the potential use of Coix lacryma-jobi seeds as a skin whitening agent and reveal the seeds to be a rich source of important phytochemicals with melanogenesis inhibitory activity...
February 16, 2017: Natural Product Research
https://www.readbyqxmd.com/read/28278313/distribution-of-content-in-recently-viewed-scenes-whitens-perception
#9
April M Schweinhart, Patrick Shafto, Edward A Essock
Anisotropies in visual perception have often been presumed to reflect an evolutionary adaptation to an environment with a particular anisotropy. Here, we adapt observers to globally-atypical environments presented in virtual reality to assess the malleability of this well-known perceptual anisotropy. Results showed that the typical bias in orientation perception was in fact altered as a result of recent experience. Application of Bayesian modeling indicates that these global changes of the recently-viewed environment implicate a Bayesian prior matched to the recently experienced environment...
March 1, 2017: Journal of Vision
https://www.readbyqxmd.com/read/28278191/effect-of-tooth-whitening-strips-on-fatigue-resistance-and-flexural-strength-of-bovine-dentin-in-vitro
#10
Laura E Tam, Namhee Kim, Grace M De Souza
OBJECTIVE: To determine the effects of whitening strips on bovine dentin fatigue resistance and flexural strength in vitro. MATERIALS AND METHODS: A total of eighty bovine dentin specimens (2x2x17mm) were treated with either: control glycerine gel on plastic film wrap or whitening strips containing 9.5% hydrogen peroxide. Treatment was applied for 30 minutes, twice a day, for 1- or 4-weeks. After the last treatment, ten specimens per group were randomly selected to undergo fatigue testing (106 cycles, 3Hz, 20N) while the other ten were subjected to flexural strength testing after ten days of storage in artificial saliva...
2017: PloS One
https://www.readbyqxmd.com/read/28275277/attitudes-of-students-of-differenet-schools-of-university-of-zagreb-on-tooth-bleaching
#11
Dinka Diklić, Eva Klarić Sever, Nada Galić, Jelena Spajić, Katica Prskalo
OBJECTIVE: To compare the awareness that students from four different faculties within the University of Zagreb have of oral health and tooth bleaching procedure. MATERIALS AND METHODS: The study included 158 subjects (both male and female) - 38 students from the School of Dental Medicine and 40 students from each of the following faculties: the School of Medicine, the Faculty of Economics and the Faculty of Civil Engineering. The respondents were asked to fill out the survey with multiple choices by marking the answers they considered correct...
December 2016: Acta Stomatologica Croatica
https://www.readbyqxmd.com/read/28268138/chemical-and-bioactivity-of-flavanones-obtained-from-roots-of-dalea-pazensis-rusby
#12
María D Santi, Mariana A Peralta, Caterine S Mendoza, José Luis Cabrera, María G Ortega
Two new prenylated flavanones, pazentin A (3',4'-dihydroxy-6,2'-diprenylpinocembrin, 1) and pazentin B [4'-hydroxy-2'-methoxy-5'-(1‴, 1‴-dimethylallyl)-6-prenylpinocembrin, 2] together with two known ones (3 and 4) previously isolated from other Dalea species were obtained from the benzene extract of Dalea pazensis Rusby roots. The compounds were evaluated in vitro for their inhibition on mushroom tyrosinase enzyme and in relation to their effect on melanogenesis in B16 murine melanoma cells, by using a spectrophotometric method...
February 27, 2017: Bioorganic & Medicinal Chemistry Letters
https://www.readbyqxmd.com/read/28259254/effects-of-boiling-duration-in-processing-of-white-paeony-root-on-its-overall-quality-evaluated-by-ultra-high-performance-liquid-chromatography-quadrupole-time-of-flight-mass-spectrometry-based-metabolomics-analysis-and-high-performance-liquid-chromatography
#13
Kong Ming, Jun Xu, Huan-Huan Liu, Jin-Di Xu, Xiu-Yang Li, Min Lu, Chun-Ru Wang, Hu-Biao Chen, Song-Lin Li
Boiling processing is commonly used in post-harvest handling of White Paeony Root (WPR), in order to whiten the herbal materials and preserve the bright color, since such WPR is empirically considered to possess a higher quality. The present study was designed to investigate whether and how the boiling processing affects overall quality of WPR. First, an ultra-high performance liquid chromatography quadrupole/time-of-flight mass spectrometry-based metabolomics approach coupled with multivariate statistical analysis was developed to compare the holistic quality of boiled and un-boiled WPR samples...
January 2017: Chinese Journal of Natural Medicines
https://www.readbyqxmd.com/read/28258753/spatial-and-temporal-evolution-of-climatic-factors-and-its-impacts-on-potential-evapotranspiration-in-loess-plateau-of-northern-shaanxi-china
#14
C Li, P T Wu, X L Li, T W Zhou, S K Sun, Y B Wang, X B Luan, X Yu
Agriculture is very sensitive to climate change, and correct forecasting of climate change is a great help to accurate allocation of irrigation water. The use of irrigation water is influenced by crop water demand and precipitation. Potential evapotranspiration (ET0) is a measure of the ability of the atmosphere to remove water from the surface through the processes of evaporation and transpiration, assuming no control on water supply. It plays an important role in assessing crop water requirements, regional dry-wet conditions, and other factors of water resource management...
March 1, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/28242773/adipose-specific-deletion-of-kif5b-exacerbates-obesity-and-insulin-resistance-in-a-mouse-model-of-diet-induced-obesity
#15
Ju Cui, Jing Pang, Ya-Jun Lin, Huan Gong, Zhen-He Wang, Yun-Xuan Li, Jin Li, Zai Wang, Ping Jiang, Da-Peng Dai, Jian Li, Jian-Ping Cai, Jian-Dong Huang, Tie-Mei Zhang
Recent studies have shown that KIF5B (conventional kinesin heavy chain) mediates glucose transporter type 4 translocation and adiponectin secretion in 3T3-L1 adipocytes, suggesting an involvement of KIF5B in the homeostasis of metabolism. However, the in vivo physiologic function of KIF5B in adipose tissue remains to be determined. In this study, adipose-specific Kif5b knockout (F-K5bKO) mice were generated using the Cre-LoxP strategy. F-K5bKO mice had similar body weights to controls fed on a standard chow diet...
February 27, 2017: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
https://www.readbyqxmd.com/read/28236822/a-simple-two-step-cloud-point-extraction-process-for-removing-fluorescent-whitening-agents-vbl-in-industrial-wastewater-and-recycling-of-surfactant
#16
Juan Han, Xu Tang, Yun Wang, Jing Li, Liang Ni, Lei Wang
  With the enhancement of people's environmental consciousness, the treatment of wastewater was studied as the focus of this paper. Here we present a simple two-step extraction to realize efficient separation of fluorescent whitening agents VBL and cyclic utilization of surfactant to reduce the cost of wastewater treatment and environmental pollution. Firstly, the removal of VBL has been achieved by CPE using TX-114 as nonionic surfactant. The results showed that complete extraction was possible using 1% (w/w) TX-114 for VBL concentration not exceeding 17...
March 1, 2017: Water Environment Research: a Research Publication of the Water Environment Federation
https://www.readbyqxmd.com/read/28222156/toothbrush-abrasivity-in-a-long-term-simulation-on-human-dentin-depends-on-brushing-mode-and-bristle-arrangement
#17
Mozhgan Bizhang, Ilka Schmidt, Yong-Hee Patricia Chun, Wolfgang H Arnold, Stefan Zimmer
OBJECTIVE: The aim of this study was to evaluate the susceptibility of dentin to brushing abrasion using four different toothbrushes (rotating-oscillating, sonic and two types of manual toothbrushes) with the same brushing forces. METHODS: Dentin samples (n = 72) were selected from 72 impacted third molars. Half of the surface of dentin samples was covered with an adhesive tape, creating a protected and a freely exposed area in the same specimen. Brushing was performed with either a: sonic (Sonicare PowerUp, Philips GmbH, Hamburg, Germany), b: oscillating-rotating (Oral B Vitality Precisions Clean, Procter & Gamble, Schwalbach am Taunus, Germany) or two different manual toothbrushes c: flat trim brush head toothbrush (Dr...
2017: PloS One
https://www.readbyqxmd.com/read/28219012/tyrosinase-catalyzed-oxidation-of-the-leukoderma-inducing-agent-raspberry-ketone-produces-e-4-3-oxo-1-butenyl-1-2-benzoquinone-implications-for-melanocyte-toxicity
#18
Shosuke Ito, Maki Hinoshita, Erina Suzuki, Makoto Ojika, Kazumasa Wakamatsu
The exposure of human skin to 4-(4-hydroxyphenyl)-2-butanone (raspberry ketone, RK) is known to cause chemical/occupational leukoderma. RK has a structure closely related to 4-(4-hydroxyphenyl)-2-butanol (rhododendrol), a skin whitening agent that was found to cause leukoderma in the skin of consumers in 2013. Rhododendrol is a good substrate for tyrosinase and causes a tyrosinase-dependent cytotoxicity to melanocytes, cells that are responsible for skin pigmentation. Therefore, it is expected that RK exerts its cytotoxicity to melanocytes through the tyrosinase-catalyzed oxidation to cytotoxic o-quinones...
March 6, 2017: Chemical Research in Toxicology
https://www.readbyqxmd.com/read/28198460/automated-detection-of-malarial-retinopathy-in-digital-fundus-images-for-improved-diagnosis-in-malawian-children-with-clinically-defined-cerebral-malaria
#19
Vinayak Joshi, Carla Agurto, Simon Barriga, Sheila Nemeth, Peter Soliz, Ian J MacCormick, Susan Lewallen, Terrie E Taylor, Simon P Harding
Cerebral malaria (CM), a complication of malaria infection, is the cause of the majority of malaria-associated deaths in African children. The standard clinical case definition for CM misclassifies ~25% of patients, but when malarial retinopathy (MR) is added to the clinical case definition, the specificity improves from 61% to 95%. Ocular fundoscopy requires expensive equipment and technical expertise not often available in malaria endemic settings, so we developed an automated software system to analyze retinal color images for MR lesions: retinal whitening, vessel discoloration, and white-centered hemorrhages...
February 15, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28195092/efficacy-of-in-house-fluorescent-stain-for-fungus
#20
K R L Surya Kirani, V Satya Chandrika
CONTEXT: Mycotic infections are gaining importance in the present day medicine, and definite demonstration of fungus is essential for diagnosis. Small numbers of organisms in the smear can be identified by fluorescence microscopy. Calcofluor white (CFW) fluorescent stain is a textile brightener mixed with Evans blue. It is expensive and not easily available. AIMS: (1) To assess the efficacy of in-house CFW fluorescent stain for fungus in relation to conventional CFW stain, histopathology, and culture...
January 2017: Indian Journal of Pathology & Microbiology
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