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https://www.readbyqxmd.com/read/28624679/neuraminidase-based-recombinant-virus-like-particles-protect-against-lethal-avian-influenza-a-h5n1-virus-infection-in-ferrets
#1
Gale E Smith, Xiangjie Sun, Yaohui Bai, Ye V Liu, Michael J Massare, Melissa B Pearce, Jessica A Belser, Taronna R Maines, Hannah M Creager, Gregory M Glenn, David Flyer, Peter Pushko, Min Z Levine, Terrence M Tumpey
Avian influenza A (H5N1) viruses represent a growing threat for an influenza pandemic. The presence of widespread avian influenza virus infections further emphasizes the need for vaccine strategies for control of pre-pandemic H5N1 and other avian influenza subtypes. Influenza neuraminidase (NA) vaccines represent a potential strategy for improving vaccines against avian influenza H5N1 viruses. To evaluate a strategy for NA vaccination, we generated a recombinant influenza virus-like particle (VLP) vaccine comprised of the NA protein of A/Indonesia/05/2005 (H5N1) virus...
June 15, 2017: Virology
https://www.readbyqxmd.com/read/28612701/neuraminidase-mediated-haemagglutination-of-recent-human-influenza-a-h3n2-viruses-is-determined-by-arginine-150-flanking-the-neuraminidase-catalytic-site
#2
Ramona Mögling, Mathilde J Richard, Stefan van der Vliet, Ruud van Beek, Eefje J A Schrauwen, Monique I Spronken, Guus F Rimmelzwaan, Ron A M Fouchier
Over the last decade, an increasing proportion of circulating human influenza A(H3N2) viruses exhibited haemagglutination activity that was sensitive to neuraminidase inhibitors. This change in haemagglutination as compared to older circulating A(H3N2) viruses prompted an investigation of the underlying molecular basis. Recent human influenza A(H3N2) viruses were found to agglutinate turkey erythrocytes in a manner that could be blocked with either oseltamivir or neuraminidase-specific antisera, indicating that agglutination was driven by neuraminidase, with a low or negligible contribution of haemagglutinin...
June 13, 2017: Journal of General Virology
https://www.readbyqxmd.com/read/28607101/positive-and-negative-control-pathways-by-blood-components-for-intermedilysin-production-in-streptococcus-intermedius
#3
Toshifumi Tomoyasu, Takahiro Yamasaki, Shinya Chiba, Shingo Kusaka, Atsushi Tabata, Robert A Whiley, Hideaki Nagamune
Streptococcus intermedius is an opportunistic bacterial pathogen secreting a human-specific cytolysin called intermedilysin (ILY) as a major pathogenic factor. This bacterium can degrade glycans into monosaccharides using two glycosidases, multisubstrate glycosidase A (MsgA) and neuraminidase (NanA). Here, we detected a strong hemolytic activity mediated by ILY when Sintermedius PC574 was cultured in fetal bovine serum (FBS) as compared to the standard culture medium. FBS-cultured cells also showed higher MsgA and NanA activity although overproduction of ILY in FBS was undetectable in mutants nanA-null and msgA-null...
June 12, 2017: Infection and Immunity
https://www.readbyqxmd.com/read/28603679/galactosialidosis-historic-aspects-and-overview-of-investigated-and-emerging-treatment-options
#4
Ida Annunziata, Alessandra d'Azzo
INTRODUCTION: Galactosialidosis is a glycoprotein storage disease caused by mutations in the CTSA gene, encoding lysosomal protective protein/cathepsin A (PPCA). The enzyme's catalytic activity is distinct from its protective function towards β-galactosidase (β-GAL) and neuraminidase 1 (NEU1), with which PPCA forms a complex. In this configuration the two glycosidases acquire their full activity and stability in lysosomes. Deficiency of PPCA results in combined NEU1/β-GAL deficiency...
2017: Expert Opinion on Orphan Drugs
https://www.readbyqxmd.com/read/28597818/enhanced-pathogenicity-and-neurotropism-of-mouse-adapted-h10n7-influenza-virus-are-mediated-by-novel-pb2-and-na-mutations
#5
Xuxiao Zhang, Guanlong Xu, Chenxi Wang, Ming Jiang, Weihua Gao, Mingyang Wang, Honglei Sun, Yipeng Sun, Kin-Chow Chang, Jinhua Liu, Juan Pu
The H10 subtype of avian influenza viruses (AIVs) circulates globally in wild birds and poultry, and this subtype has been shown to be increasingly prevalent in China. Among the various H10 viruses, H10N7 AIVs have caused repeated mammal and human infections. To investigate their genetic adaptation in mammals, we generated a mouse-adapted avian H10N7 variant (A/mallard/Beijing/27/2011-MA; BJ27-MA) which exhibited increased virulence in mice compared to wild-type virus and acquired neurotropism. Sequencing showed the absence of the widely recognized mammalian adaptation markers of E627K and D701N in PB2 in the mouse-adapted strain; instead, five amino acid mutations were identified: E158G and M631L in PB2; G218E in haemagglutinin (H3 numbering); and K110E and S453I in neuraminidase (NA)...
June 8, 2017: Journal of General Virology
https://www.readbyqxmd.com/read/28592535/newcastle-disease-virus-establishes-persistent-infection-in-tumor-cells-in-vitro-contribution-of-the-cleavage-site-of-fusion-protein-and-second-sialic-acid-binding-site-of-hemagglutinin-neuraminidase
#6
Udaya S Rangaswamy, Weijia Wang, Xing Cheng, Patrick McTamney, Danielle Carroll, Hong Jin
Newcastle Disease Virus (NDV) is an oncolytic virus being developed for the treatment of cancer. Following infection of an ovarian human cancer cell line (OVCAR3) with a recombinant low pathogenic NDV, persistent infection (PI) was established in a subset of tumor cells. PI cells exhibited resistance to superinfection with NDV, and an anti-viral state as demonstrated by upregulation of interferon and interferon induced genes such as Myxoma resistance gene 1 (Mx1) and Retinoic acid-inducing gene-I (RIG-I). Viruses released from PI cells induced higher cell to cell fusion following infection compared to the parental virus in two tumor cell lines tested, HT1080 and HeLa, and remained to be attenuated in chickens...
June 7, 2017: Journal of Virology
https://www.readbyqxmd.com/read/28587867/spatiotemporal-dynamics-of-influenza-a-h1n1-pdm09-in-brazil-during-the-pandemic-and-post-pandemic-periods
#7
Alessandra C B Manito, Tiago Gräf, Vagner R Lunge, Nilo Ikuta
Influenza A(H1N1)pdm09 was responsible for the first global flu pandemic in 21st century affecting all the world. In Brazil, A(H1N1)pdm09 is still circulating as a seasonal virus, causing deaths every year. Nevertheless, the viral diffusion process that yearly seeds new influenza strains in the country was not investigated yet. The aim of the current study was to describe the phylodynamics and phylogeography of influenza A(H1N1)pdm09 in Brazil between 2009 and 2014. Neuraminidase sequences from Brazil and other regions of the World were retrieved and analyzed...
June 3, 2017: Virus Research
https://www.readbyqxmd.com/read/28581345/development-and-identification-of-a-new-vero-cell-based-live-attenuated-influenza-b-vaccine-by-a-modified-classical-reassortment-method
#8
Fan Yang, Lei Ma, Jian Zhou, Yinjie Wu, Jingxia Gao, Shaohui Song, Xingliang Geng, Qi Guo, Zhuofan Li, Weidong Li, Guoyang Liao
OBJECTIVE: The goal of this study was to generate a new Vero and cold-adapted live attenuated influenza B vaccine and evaluate its safety and immunogenicity with experiments in a guinea pig model. METHODS: A modified classical reassortment method was used to generate the new vaccine strain. The donor strain was B/Yunnan/2/2005Vca(B), and the parental virus strain was B/Brisbane/60/2008wt. After co-infection in Vero cells, the prepared antibody serum inhibited the donor strain growth, and screening conditions inhibited the candidate virus growth, which induced the growth of the new reassortant virus B/Brisbane/60/2008Vca(B) grow...
June 5, 2017: Expert Review of Vaccines
https://www.readbyqxmd.com/read/28580899/human-infection-with-highly-pathogenic-avian-influenza-a-h7n9-virus-china
#9
Changwen Ke, Chris Ka Pun Mok, Wenfei Zhu, Haibo Zhou, Jianfeng He, Wenda Guan, Jie Wu, Wenjun Song, Dayan Wang, Jiexiong Liu, Qinhan Lin, Daniel Ka Wing Chu, Lei Yang, Nanshan Zhong, Zifeng Yang, Yuelong Shu, Joseph Sriyal Malik Peiris
The recent increase in zoonotic avian influenza A(H7N9) disease in China is a cause of public health concern. Most of the A(H7N9) viruses previously reported have been of low pathogenicity. We report the fatal case of a patient in China who was infected with an A(H7N9) virus having a polybasic amino acid sequence at its hemagglutinin cleavage site (PEVPKRKRTAR/GL), a sequence suggestive of high pathogenicity in birds. Its neuraminidase also had R292K, an amino acid change known to be associated with neuraminidase inhibitor resistance...
July 17, 2017: Emerging Infectious Diseases
https://www.readbyqxmd.com/read/28580101/highly-specific-and-rapid-glycan-based-amperometric-detection-of-influenza-viruses
#10
Xikai Cui, Amrita Das, Abasaheb N Dhawane, Joyce Sweeney, Xiaohu Zhang, Vasanta Chivukula, Suri S Iyer
Rapid and precise detection of influenza viruses in a point of care setting is critical for applying appropriate countermeasures. Current methods such as nucleic acid or antibody based techniques are expensive or suffer from low sensitivity, respectively. We have developed an assay that uses glucose test strips and a handheld potentiostat to detect the influenza virus with high specificity. Influenza surface glycoprotein neuraminidase (NA), but not bacterial NA, cleaved galactose bearing substrates, 4,7di-OMe N-acetylneuraminic acid attached to the 3 or 6 position of galactose, to release galactose...
May 1, 2017: Chemical Science
https://www.readbyqxmd.com/read/28573862/fluorinated-amine-stereotriads-via-allene-amination
#11
Lu Liu, Nels C Gerstner, Lucas J Oxtoby, Ilia A Guzei, Jennifer M Schomaker
The incorporation of fluorine into organic scaffolds often improves the bioactivity of pharmaceutically relevant compounds. C-F/C-N/C-O stereotriad motifs are prevalent in antivirals, neuraminidase inhibitors, and modulators of androgen receptors, but are challenging to install. An oxidative allene amination strategy using Selectfluor rapidly delivers triply functionalized triads of the form C-F/C-N/C-O, exhibiting good scope and diastereoselectivity for all syn products. The resulting stereotriads are readily transformed into fluorinated pyrrolidines and protected α-, β-, and γ-amino acids...
June 2, 2017: Organic Letters
https://www.readbyqxmd.com/read/28561264/virulence-markers-associated-with-trueperella-pyogenes-infections-in-livestock-and-companion-animals
#12
R M Risseti, E Zastempowska, M Twarużek, H Lassa, J C F Pantoja, A P C de Vargas, S T Guerra, C A D Bolaños, C L de Paula, A C Alves, B S Colhado, F V R Portilho, C Tasca, G H B Lara, M G Ribeiro
Trueperella pyogenes is an opportunistic pathogen that causes diverse pyogenic infections in livestock. The genes that encode the exotoxin pyolysin (plo) and other putative factors that promote adhesion of pathogen to host cells (fimbriae fimA, fimC, fimE, fimG, neuraminidases nanH, nanP, and collagen-binding protein cbpA) have been associated with virulence, particularly in mastitis and uterus infections of dairy cows. However, the role of these virulence markers in the pathogenicity of the agent in domestic animals infections still is incompletely understood...
May 31, 2017: Letters in Applied Microbiology
https://www.readbyqxmd.com/read/28555253/a-turkish-case-of-galactosialidosis-with-a-new-homozygous-mutation-in-ctsa-gene
#13
Ayşe Kartal, Kürşad Aydın
Galactosialidosis is an autosamal reressive lysosomal storage disease caused by a combined deficiency of lysosomal β-galactosidase and neuraminidase, due to a primary defect in protective protein/cathepsin A. Three subtypes are recognized: the early infantile type, the late infantile type, and the juvenile/adult type. We report here a female patient with early infantile galactosialidosis who was born at 35 weeks of gestation. After birth she remained at the neonatal intensive care unit. Physical examination revealed, coarse facial features, hepatomegaly, cardiac murmur and diffuse hypotonia...
May 30, 2017: Metabolic Brain Disease
https://www.readbyqxmd.com/read/28551451/a-sandwich-elisa-for-the-detection-of-neuraminidase-of-avian-influenza-a-h7n9-virus
#14
Yueyang Yu, Xi Zhang, Baihui Zhao, Ying Sun, Xiaoguang Zhang, Tian Bai, Jian Lu, Zi Li, Liqi Liu, Dayan Wang, Yuelong Shu, Jianfang Zhou, Kun Qin
Although exhibiting no or low virulence in poultry, avian influenza virus H7N9 has caused around 1400 confirmed human infections in China with a case-fatality rate of 30% since 2013. A highly pathogenic H7N9 virus (HP-H7), with the HA antigenicity distinct from the previous, were recently detected in patients and poultry. Therefore, convenient rapid diagnosis with reliability will allow early antiviral use and management for H7N9 infection. Here, a sandwich ELISA targeting the conserved viral antigen, neuraminidase (NA) was developed...
May 25, 2017: Journal of Virological Methods
https://www.readbyqxmd.com/read/28550754/linear-polysialoside-outperforms-dendritic-analogs-for-inhibition-of-influenza-virus-infection-in%C3%A2-vitro-and-in%C3%A2-vivo
#15
Sumati Bhatia, Daniel Lauster, Markus Bardua, Kai Ludwig, Stefano Angioletti-Uberti, Nicole Popp, Ute Hoffmann, Florian Paulus, Matthias Budt, Marlena Stadtmüller, Thorsten Wolff, Alf Hamann, Christoph Böttcher, Andreas Herrmann, Rainer Haag
Inhibition of influenza A virus infection by multivalent sialic acid inhibitors preventing viral hemagglutinin binding to host cells of the respiratory tract is a promising strategy. However, optimal geometry and optimal ligand presentation on multivalent scaffolds for efficient inhibition both in vitro and in vivo application are still unclear. Here, by comparing linear and dendritic polyglycerol sialosides (LPGSA and dPGSA) we identified architectural requirements and optimal ligand densities for an efficient multivalent inhibitor of influenza virus A/X31/1 (H3N2)...
May 20, 2017: Biomaterials
https://www.readbyqxmd.com/read/28539063/a-qsar-classification-model-for-neuraminidase-inhibitors-of-influenza-a-viruses-h1n1-based-on-weighted-penalized-support-vector-machine
#16
Z Y Algamal, M K Qasim, H T M Ali
Descriptor selection is a procedure widely used in chemometrics. The aim is to select the best subset of descriptors relevant to the quantitative structure-activity relationship (QSAR) study being considered. In this paper, a new descriptor selection method for the QSAR classification model is proposed by adding a new weight inside L1-norm. The experimental results from classifying the neuraminidase inhibitors of influenza A viruses (H1N1) demonstrate that the proposed method in the QSAR classification model performs effectively and competitively compared with other existing penalized methods in terms of classification performance and the number of selected descriptors...
May 2017: SAR and QSAR in Environmental Research
https://www.readbyqxmd.com/read/28537546/biological-characterisation-of-the-emerged-highly-pathogenic-avian-influenza-hpai-a-h7n9-viruses-in-humans-in-mainland-china-2016-to-2017
#17
Wenfei Zhu, Jianfang Zhou, Zi Li, Lei Yang, Xiyan Li, Weijuan Huang, Sumei Zou, Wenbing Chen, Hejiang Wei, Jing Tang, Liqi Liu, Jie Dong, Dayan Wang, Yuelong Shu
With no or low virulence in poultry, avian influenza A(H7N9) virus has caused severe infections in humans. In the current fifth epidemic wave, a highly pathogenic avian influenza (HPAI) H7N9 virus emerged. The insertion of four amino acids (KRTA) at the haemagglutinin (HA) cleavage site enabled trypsin-independent infectivity of this virus. Although maintaining dual receptor-binding preference, its HA antigenicity was distinct from low-pathogenic avian influenza A(H7N9). The neuraminidase substitution R292K conferred a multidrug resistance phenotype...
May 11, 2017: Euro Surveillance: Bulletin Européen sur les Maladies Transmissibles, European Communicable Disease Bulletin
https://www.readbyqxmd.com/read/28536876/design-and-one-pot-synthesis-of-2-thiazolylhydrazone-derivatives-as-influenza-neuraminidase-inhibitors
#18
Keyang Yuan, Mengwu Xiao, Ying Tan, Jiao Ye, Yongle Xie, Xiaoxiao Sun, Aixi Hu, Wenwen Lian, Ailin Liu
Two series of novel 2-thiazolylhydrazone derivatives were designed and synthesized via one-pot reaction of benzaldehyde derivatives, [Formula: see text]-haloketones and thiosemicarbazide. The structures of compounds 1 and 2 were characterized by [Formula: see text] NMR and [Formula: see text] NMR, and compound 1g was further confirmed by X-ray crystallography. All of the target compounds were evaluated for their NA inhibitory activity against influenza viral neuraminidase (H1N1) in vitro, and the results showed that many compounds exhibited moderate to strong inhibitory activities against influenza viral neuraminidase (H1N1)...
May 23, 2017: Molecular Diversity
https://www.readbyqxmd.com/read/28534230/trueperella-pyogenes-isolated-from-a-brain-abscess-of-an-adult-roebuck-capreolus-capreolus
#19
Jörn-Peter Wickhorst, Abdulwahed Ahmed Hassan, Omar Hashim Sheet, Tobias Eisenberg, Osama Sammra, Mazen Alssahen, Christoph Lämmler, Ellen Prenger-Berninghoff, Michael Zschöck, Markus Timke, Amir Abdulmawjood
The present study was designed to characterize phenotypically and genotypically a Trueperella pyogenes strain isolated from a brain abscess of an adult roebuck (Capreolus capreolus). The species identity could be confirmed by phenotypical investigations, by MALDI-TOF MS analysis, and by sequencing the 16S ribosomal RNA (rRNA) gene, the 16S-23S rRNA intergenic spacer region (ISR); by sequencing the target genes rpoB, gap, and tuf; and by detection of T. pyogenes chaperonin-encoding gene cpn60 with a previously developed loop-mediated isothermal amplification (LAMP) assay...
May 23, 2017: Folia Microbiologica
https://www.readbyqxmd.com/read/28533468/vacuolation-activity-and-intracellular-trafficking-of-artb-the-binding-subunit-of-an-ab5-toxin-produced-by-salmonella-enterica-serovar-typhi
#20
Brock P Herdman, James C Paton, Hui Wang, Travis Beddoe, Adrienne W Paton
Various Salmonella enterica serovars, including S Typhi, encode an AB5 toxin (ArtAB), the A subunit of which is an ADP-ribosyltransferase related to the S1 subunit of pertussis toxin. However, although the A subunit is able to catalyse ADP-ribosylation of host G proteins, a cytotoxic phenotype has yet to be identified for the holotoxin. Here we show that its B subunit pentamer (ArtB) binds to receptors on the surface of Vero (African green monkey kidney), CHO (Chinese hamster ovary), U937 (human monocyte) and HBMEC (human brain microvascular endothelial) cell lines...
May 22, 2017: Infection and Immunity
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