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Nataly De Jesús Huertas Méndez, Yerly Vargas Casanova, Anyelith Katherine Gómez Chimbi, Edith Hernández, Aura Lucia Leal Castro, Javier Mauricio Melo Diaz, Zuly Jenny Rivera Monroy, Javier Eduardo García Castañeda
Linear, dimeric, tetrameric, and cyclic peptides derived from lactoferricin B-containing non-natural amino acids and the RWQWR motif were synthesized, purified, and characterized using RP-HPLC, MALDI-TOF mass spectrometry, and circular dichroism. The antibacterial activity of peptides against Escherichia coli ATCC 11775, Stenotrophomonas maltophilia ATCC 13636, and Salmonella enteritidis ATCC 13076 was evaluated. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were determined...
March 12, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
Ya Hao, Na Yang, Xiumin Wang, Da Teng, Ruoyu Mao, Xiao Wang, Zhanzhan Li, Jianhua Wang
Bovine lactoferricin (LfcinB) has potent antibacterial, antifungal and antiparasitic activities but is also hemolytic. Our objective was to identify LfcinB17-31 derivatives with reduced hemolysis and improved antimicrobial activity via substituting Cys3, Arg4, Gln7, Met10, and Gly14 with more hydrophobic residues. Two peptides, Lfcin4 and Lfcin5, showed higher activity against Staphylococcus aureus and Salmonella enteritidis and lower hemolytic activity than the parent peptide LfcinB17-31. These peptides permeabilized the outer and inner membranes of S...
March 13, 2017: Scientific Reports
Hsiu-Chin Huang, Hsuan Lin, Min-Chuan Huang
Lactoferricin B (LfcinB), a peptide of bovine lactoferrin (LfB), exhibits multiple biological functions, including antimicrobial, antiviral, antioxidant and immunomodulatory activities. However, the role of LfcinB-related peptides in melanogenesis remains unclear. In this study, a set of five LfcinB-related peptides was examined. We found that LfB17‑34, an 18-mer LfcinB-derived peptide, increased melanogenesis in B16F10 melanoma cells without significantly affecting cell viability. LfB17‑34 increased in vitro tyrosinase activity and melanin content in a dose-dependent manner...
March 2017: International Journal of Molecular Medicine
Amber M Bates, Jorge L Garaicoa, Carol L Fischer, Kim A Brogden
The underlying causes of denture stomatitis may be related to the long-term use of adhesives, which may predispose individuals to oral candidiasis. In this study, we hypothesize that antimicrobial peptides and antifungal antibiotics have diminished anti-Candida activities in denture adhesive. To show this, nine antimicrobial peptides and five antifungal antibiotics with and without 1.0% denture adhesive were incubated with Candida albicans strains ATCC 64124 and HMV4C in radial diffusion assays. In gels with 1...
February 6, 2017: Antibiotics
Rulan Jiang, Bo Lönnerdal
Lactoferrin (Lf) is an iron-binding glycoprotein that is present at high concentrations in milk. Bovine lactoferricin (LfcinB) is a peptide fragment generated by pepsin proteolysis of bovine lactoferrin (bLf). LfcinB consists of amino acid residues 17-41 proximal to the N-terminus of bLf and a disulfide bond between residues 19 and 36, forming a loop. Both bLf and LfcinB have been demonstrated to have antitumor activities. Colorectal cancer is the second most common cause of cancer death in developed countries...
February 2017: Biochemistry and Cell Biology, Biochimie et Biologie Cellulaire
Mauricio Arias, Ashley L Hilchie, Evan F Haney, Jan G M Bolscher, M Eric Hyndman, Robert E W Hancock, Hans J Vogel
Lactoferrin (LF) is a mammalian host defense glycoprotein with diverse biological activities. Peptides derived from the cationic region of LF possess cytotoxic activity against cancer cells in vitro and in vivo. Bovine lactoferricin (LFcinB), a peptide derived from bovine LF (bLF), exhibits broad-spectrum anticancer activity, while a similar peptide derived from human LF (hLF) is not as active. In this work, several peptides derived from the N-terminal regions of bLF and hLF were studied for their anticancer activities against leukemia and breast-cancer cells, as well as normal peripheral blood mononuclear cells...
February 2017: Biochemistry and Cell Biology, Biochimie et Biologie Cellulaire
Kenya E Fernandes, Dee A Carter
Lactoferrin is a multifunctional iron-binding glycoprotein belonging to the transferrin family. It is found abundantly in milk and is present as a major protein in human exocrine secretions where it plays a role in the innate immune response. Various antifungal functions of lactoferrin have been reported including a wide spectrum of activity across yeasts and molds and synergy with other antifungal drugs in combination therapy, and various modes of action have been proposed. Bioactive peptides derived from lactoferrin can also exhibit strong antifungal activity, with some surpassing the potency of the whole protein...
2017: Frontiers in Microbiology
Lixiang Meng, Geliang Xu, Jiansheng Li, Wenbin Liu, Weidong Jia, Jinliang Ma, Decheng Wei
Bovine lactoferricin P13 (LfcinB-P13) is a peptide derived from LfcinB. In the present study, the effect of LfcinB-P13 on the human liver cancer cell line SMMC7721 was investigated in vitro and in vivo. The results of the present study indicate that LfcinB-P13 significantly decreased SMMC7721 cell viability in vitro (P=0.032 vs. untreated cells), while exhibiting low cytotoxicity in the wild-type liver cell line L02. In addition, the rate of apoptosis in SMMC7721 cells was significantly increased following treatment with 40 and 60 µg/ml LfcinB-P13 (P=0...
January 2017: Oncology Letters
Paola Morici, Roberta Fais, Cosmeri Rizzato, Arianna Tavanti, Antonella Lupetti
The aim of this study was to evaluate the in vitro activity of the synthetic peptide hLF1-11 against biofilm produced by clinical isolates of Candida albicans with different fluconazole susceptibility. The antibiofilm activity of the peptide hLF1-11 was assessed in terms of reduction of biofilm cellular density, metabolic activity and sessile cell viability. The extent of morphogenesis in hLF1-11 treated and untreated biofilms was also investigated microscopically. Transcription levels of genes related to cell adhesion, hyphal development and extracellular matrix production were analysed by qRT-PCR in hLF1-11 treated and untreated biofilms...
2016: PloS One
Jorge L Garaicoa, Carol L Fischer, Amber M Bates, Julie Holloway, Gustavo Avila-Ortiz, Janet M Guthmiller, Georgia K Johnson, Clark Stanford, Kim A Brogden
PURPOSE: Several complications may arise in patients wearing complete prosthetic appliances, including denture-associated infections and mucosal stomatitis due to Candida species. This study evaluated the activity of anti-Candida agents in denture adhesive and the cytotoxicities of these preparations for primary human gingival epithelial (GE) keratinocytes. MATERIALS AND METHODS: The anti-Candida activities of antimicrobial peptides, antimicrobial lipids, and antifungal agents against C...
November 21, 2016: Journal of Prosthodontics: Official Journal of the American College of Prosthodontists
Tjitske Sijbrandij, Antoon J Ligtenberg, Kamran Nazmi, Enno C I Veerman, Jan G M Bolscher, Floris J Bikker
Lactoferrin (LF) is an important immune protein in neutrophils and secretory fluids of mammals. Bovine LF (bLF) harbours two antimicrobial stretches, lactoferricin and lactoferampin, situated in close proximity in the N1 domain. To mimic these antimicrobial domain parts a chimeric peptide (LFchimera) has been constructed comprising parts of both stretches (LFcin17-30 and LFampin265-284). To investigate the potency of this construct to combat a set of Gram positive and Gram negative bacteria which are regarded as simulants for biological warfare agents, the effect on bacterial killing, membrane permeability and membrane polarity were determined in comparison to the constituent peptides and the native bLF...
January 2017: World Journal of Microbiology & Biotechnology
Renxun Chen, Nerida Cole, Debarun Dutta, Naresh Kumar, Mark D P Willcox
Lactoferrin and lactoferricin were immobilized on glass surfaces via two linkers, 4-azidobenzoic acid (ABA) or 4-fluoro-3-nitrophenyl azide (FNA). The resulting surfaces were characterized by X-ray photoelectron spectroscopy (XPS) and contact angle measurements. The antimicrobial activity of the surfaces was determined using Pseudomonas aeruginosa and Staphylococcus aureus strains by fluorescence microscopy. Lactoferrin and lactoferricin immobilization was confirmed by XPS showing significant increases (p < 0...
October 19, 2016: Journal of Biomedical Materials Research. Part B, Applied Biomaterials
Woan-Sub Kim, Pyeung-Hyeun Kim, Kei-Ichi Shimazaki
The antimicrobial activity of bovine lactoferrin hydrolysates (bLFH) was measured against Pseudomonas strains (P. syringae and P. fluorescens) in vitro. To compare susceptibility to bLFH, minimal inhibitory concentration (MIC) values were determined using chemiluminescence assays and paper disc plate assays. Antimicrobial effect against P. fluorescens was not observed by either assay, suggesting that bLFH did not exhibit antimicrobial activity against P. fluorescens. However, a significant inhibition of P. syringae growth was observed in the presence of bLFH...
2016: Korean Journal for Food Science of Animal Resources
Leonard T Nguyen, Hans J Vogel
Staphylokinase (Sak) is a plasminogen activator protein that is secreted by many Staphylococcus aureus strains. Sak also offers protection by binding and inhibiting specific antimicrobial peptides (AMPs). Here, we evaluate Sak as a more general interaction partner for AMPs. Studies with melittin, mCRAMP, tritrpticin and bovine lactoferricin indicate that the truncation of the first ten residues of Sak (SakΔN10), which occurs in vivo and uncovers important residues in a bulge region, improves its affinity for AMPs...
2016: Scientific Reports
Yide Qin, Juan Zhou, Wenxiao Zhang, Xue Yang, Jing Wang, Cai Wei, Fang Gu, Ting Lei
Some bioactive peptides derived from natural resources or synthesized by rational design have been shown to have very good anticancer effects. We designed and prepared an anticancer fusion peptide (ACFP) through genetic engineering and studied its antiovarian cancer activity by cell culture and lentivirus infection. Bovine lactoferricin (LfcinB) and hexapeptide (PGPIPN) were connected by a flexible link arm (GGGGS) to produce an ACFP gene. ACFP was inductively expressed in Escherichia coli by the recombinant plasmid pGEX-KG-ACFP...
June 26, 2016: Anti-cancer Agents in Medicinal Chemistry
Michael B Ellman, Jaesung Kim, Howard S An, Di Chen, Ranjan Kc, Xin Li, Guozhi Xiao, Dongyao Yan, Joon Suh, Andre J van Wijnen, James H-C Wang, Su-Gwan Kim, Hee-Jeong Im
No abstract text is available yet for this article.
June 25, 2016: Gene
Natascia Bruni, Maria Teresa Capucchio, Elena Biasibetti, Enrica Pessione, Simona Cirrincione, Leonardo Giraudo, Antonio Corona, Franco Dosio
Antimicrobial peptides (AMPs) represent a vast array of molecules produced by virtually all living organisms as natural barriers against infection. Among AMP sources, an interesting class regards the food-derived bioactive agents. The whey protein lactoferrin (Lf) is an iron-binding glycoprotein that plays a significant role in the innate immune system, and is considered as an important host defense molecule. In search for novel antimicrobial agents, Lf offers a new source with potential pharmaceutical applications...
June 11, 2016: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
François Villemot, Riccardo Capelli, Giorgio Colombo, Arjan van der Vaart
Improvements to the confinement method for the calculation of conformational free energy differences are presented. By taking advantage of phase space overlap between simulations at different frequencies, significant gains in accuracy and speed are reached. The optimal frequency spacing for the simulations is obtained from extrapolations of the confinement energy, and relaxation time analysis is used to determine time steps, simulation lengths, and friction coefficients. At postprocessing, interpolation of confinement energies is used to significantly reduce discretization errors in the calculation of conformational free energies...
June 14, 2016: Journal of Chemical Theory and Computation
Neelabh, Karuna Singh, Jyoti Rani
Emerging drug resistance varieties and hyper-virulent strains of microorganisms have compelled the scientific fraternity to develop more potent and less harmful therapeutics. Antimicrobial peptides could be one of such therapeutics. This review is an attempt to explore antifungal peptides naturally produced by prokaryotes as well as eukaryotes. They are components of innate immune system providing first line of defence against microbial attacks, especially in eukaryotes. The present article concentrates on types, structures, sources and mode of action of gene-encoded antifungal peptides such as mammalian defensins, protegrins, tritrpticins, histatins, lactoferricins, antifungal peptides derived from birds, amphibians, insects, fungi, bacteria and their synthetic analogues such as pexiganan, omiganan, echinocandins and Novexatin...
June 2016: Probiotics and Antimicrobial Proteins
Winston Yan Wang, Jack Ho Wong, Denis Tsz Ming Ip, David Chi Cheong Wan, Randy Chifai Cheung, Tzi Bun Ng
This study aimed to investigate fragments derived from human and bovine lactoferrins for ability to inhibit nuclear translocation of HIV-1 integrase. It was shown that human lactoferricin, human lactoferrin 1-11, and bovine lactoferrampin reduced nuclear distribution of HIV-1 integrase. Bovine lactoferrampin could inhibit both the activity and nuclear translocation of HIV-1 integrase. Human lactoferrampin, bovine lactoferricin, and bovine lactoferrin 1-11 had no effect on HIV-1 integrase nuclear translocation...
August 2016: Applied Biochemistry and Biotechnology
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