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https://www.readbyqxmd.com/read/28107483/genetic-divergence-in-domestic-japanese-quail-inferred-from-mitochondrial-dna-d-loop-and-microsatellite-markers
#1
Mitsuo Nunome, Mikiharu Nakano, Ryo Tadano, Ryoka Kawahara-Miki, Tomohiro Kono, Shinji Takahashi, Takaharu Kawashima, Akira Fujiwara, Keijiro Nirasawa, Makoto Mizutani, Yoichi Matsuda
To assess the genetic diversity of domestic Japanese quail (Coturnix japonica) populations, and their genetic relationships, we examined mitochondrial DNA (mtDNA) D-loop sequences and microsatellite markers for 19 Japanese quail populations. The populations included nine laboratory lines established in Japan (LWC, Quv, RWN, WE, AWE, AMRP, rb-TKP, NIES-L, and W), six meat-type quail lines reimported from Western countries (JD, JW, Estonia, NIES-Br, NIES-Fr, and NIES-Hn), one commercial population in Japan, and three wild quail populations collected from three Asian areas...
2017: PloS One
https://www.readbyqxmd.com/read/28106187/layer-separable-and-gap-tunable-topological-insulators
#2
Kai-Wei Chang, Wei Ji, Chao-Cheng Kaun
The way in which states of a topological insulator (TI) transform from monolayer to bulk is an important issue for applications in spintronics. However, unlike graphite, most layered materials are difficult to exfoliate. Using first-principles calculations, we predict that thallium selenide (TlSe) will be a layered TI with rather weak interlayer coupling and thus it should be exfoliated easily. The evolution of the topological states can also be driven by doping with indium (In) atoms or applying lateral strains...
January 20, 2017: Physical Chemistry Chemical Physics: PCCP
https://www.readbyqxmd.com/read/28105847/compact-ultrahigh-pressure-nanoflow-capillary-liquid-chromatograph
#3
Xiaofeng Zhao, Xiaofeng Xie, Sonika Sharma, Luke T Tolley, Alex Plistil, Hal E Barnett, Martin P Brisbin, Adam C Swensen, John C Price, Paul B Farnsworth, H Dennis Tolley, Stanley D Stearns, Milton L Lee
A compact ultrahigh-pressure nanoflow liquid chromatograph (LC) was developed with the purpose in mind of creating a portable system that could be easily moved to various testing locations or placed in close proximity to other instruments for optimal coupling, such as with mass spectrometry (MS). The system utilized innovative nanoflow pumps integrated with a very low volume stop-flow injector and mixing tee. The system weighed only 5.9 kg (13 lbs) or 4.5 kg (10 lbs) without a controller and could hold up to 1100 bar (16000 psi) of pressure...
January 3, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28105804/cavity-enhanced-raman-spectroscopy-in-the-biosciences-in-situ-multicomponent-and-isotope-selective-gas-measurements-to-study-hydrogen-production-and-consumption-by-escherichia-coli
#4
Thomas W Smith, Michael F A Hippler
Recently we introduced Cavity-Enhanced Raman Spectroscopy (CERS) with optical feedback cw-diode lasers as a sensitive analytical tool. Here we report improvements made on the technique and its first application in the biosciences for in situ, multicomponent and isotope selective gas measurements to study hydrogen production and consumption by Escherichia coli. Under anaerobic conditions, cultures grown on rich media supplemented with D-glucose or glycerol produce H2 and simultaneously consume some of it. By introducing D2 in the headspace, hydrogen production and consumption could be separated due to the distinct spectroscopic signatures of isotopomers...
January 10, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28104375/estimate-of-opportunistic-prostate-specific-antigen-testing-in-the-finnish-randomized-study-of-screening-for-prostate-cancer
#5
Tuomas P Kilpeläinen, Dimitri Pogodin-Hannolainen, Kimmo Kemppainen, Kirsi Talala, Jani Raitanen, Kimmo Taari, Paula Kujala, Teuvo L J Tammela, Anssi Auvinen
PURPOSE: Screening for prostate cancer (PCa) remains controversial even if the European Randomized Study of Screening for Prostate Cancer (ERSPC) has shown a 21% relative reduction in PCa mortality. The Finnish Randomized Study of Screening for Prostate Cancer is the largest component of the ERSPC and showed statistically non-significant 16% mortality benefit in a separate analysis. The purpose of this study was to estimate the degree of contamination in the control arm (CA) of the Finnish trial...
January 16, 2017: Journal of Urology
https://www.readbyqxmd.com/read/28102960/nine-degrees-of-freedom-flexmap-for-a-cone-beam-computed-tomography-imaging-device-with-independently-movable-source-and-detector
#6
Peter Keuschnigg, Daniel Kellner, Karl Fritscher, Andrea Zechner, Ulrich Mayer, Philipp Huber, Felix Sedlmayer, Heinz Deutschmann, Philipp Steininger
PURPOSE: Couch-mounted cone-beam computed tomography (CBCT) imaging devices with independently rotatable x-ray source and flat-panel detector arms for acquisitions of arbitrary regions of interest (ROI) have recently been introduced in image-guided radiotherapy (IGRT). This work analyzes mechanical limitations and gravity-induced effects influencing the geometric accuracy of images acquired with arbitrary angular constellations of source and detector in nonisocentric trajectories, which is considered essential for IGRT...
January 2017: Medical Physics
https://www.readbyqxmd.com/read/28102834/basis-of-catalytic-assembly-of-the-mitotic-checkpoint-complex
#7
Alex C Faesen, Maria Thanasoula, Stefano Maffini, Claudia Breit, Franziska Müller, Suzan van Gerwen, Tanja Bange, Andrea Musacchio
Accurate genome inheritance by daughter cells requires that sister chromatids in the mother attach to microtubules emanating from opposite poles of the mitotic spindle (bi-orientation). A surveillance mechanism named the spindle assembly checkpoint (SAC) monitors the microtubule attachment process, temporarily halting sister chromatid separation and mitotic exit until completion of bi-orientation1. SAC failure results in abnormal chromosome numbers (aneuploidy), a hallmark of many tumours. The HORMA domain protein MAD2 is a subunit of the SAC effector mitotic checkpoint complex (MCC)...
January 19, 2017: Nature
https://www.readbyqxmd.com/read/28102733/plk1-polo-like-kinase-1-inhibits-mtor-complex-1-and-promotes-autophagy
#8
Stefanie Ruf, Alexander Martin Heberle, Miriam Langelaar-Makkinje, Sara Gelino, Deepti Wilkinson, Carolin Gerbeth, Jennifer Jasmin Schwarz, Birgit Holzwarth, Bettina Warscheid, Chris Meisinger, Marcel A T M van Vugt, Ralf Baumeister, Malene Hansen, Kathrin Thedieck
Mechanistic target of rapamycin complex 1 (MTORC1) and PLK1 (polo like kinase 1) are major drivers of cancer cell growth and proliferation, and inhibitors of both protein kinases are currently being investigated in clinical studies. To date, MTORC1's and PLK1's functions are mostly studied separately, and reports on their mutual crosstalk are scarce. Here, we identify PLK1 as a physical MTORC1 interactor in human cancer cells. PLK1 inhibition enhances MTORC1 activity under nutrient sufficiency and in starved cells, and PLK1 directly phosphorylates the MTORC1 component RPTOR/RAPTOR in vitro...
January 19, 2017: Autophagy
https://www.readbyqxmd.com/read/28102482/the-role-of-text-messaging-in-cardiovascular-risk-factor-optimization
#9
REVIEW
Harry Klimis, Mohammad Ehsan Khan, Cindy Kok, Clara K Chow
PURPOSE OF REVIEW: Many cases of CVD may be avoidable through lowering behavioural risk factors such as smoking and physical inactivity. Mobile health (mHealth) provides a novel opportunity to deliver cardiovascular prevention programs in a format that is potentially scalable. Here, we provide an overview of text messaging-based mHealth interventions in cardiovascular prevention. RECENT FINDINGS: Text messaging-based interventions appear effective on a range of behavioural risk factors and can effect change on multiple risk factors-e...
January 2017: Current Cardiology Reports
https://www.readbyqxmd.com/read/28101959/comprehensive-analysis-of-transcript-changes-associated-with-allograft-rejection-combining-universal-and-selective-features
#10
Philip F Halloran, Jeffery M Venner, Konrad S Famulski
We annotated the top transcripts associated with kidney transplant rejection by p-value, either universal for all rejection or selective for T cell-mediated (TCMR) or antibody-mediated rejection (ABMR). We used eight class comparison algorithms to interrogate microarray results from 703 biopsies, 205 with rejection. The positive comparators were all rejection, TCMR, or ABMR; the negative comparators varied from normal biopsies to all non-rejecting biopsies, including other diseases. The universal algorithm, rejection vs...
January 19, 2017: American Journal of Transplantation
https://www.readbyqxmd.com/read/28101745/emotional-awareness-in-depressive-and-anxiety-symptoms-in-youth-a-meta-analytic-review
#11
Lena Sendzik, Johanna Ö Schäfer, Andrea C Samson, Eva Naumann, Brunna Tuschen-Caffier
Emotion regulation is assumed to play an important role in depressive and anxiety symptoms in youth. However, the role of core components of emotion regulation, such as emotional awareness, is not well understood so far. Thus this meta-analysis aimed to examine the relationship between depressive and anxiety symptoms with emotional awareness in youth. A systematic literature search (PsycINFO, Medline, Google Scholar) identified 21 studies, from which 34 effect sizes were extracted. Results from random effects models showed that difficulties in emotional awareness were significantly correlated with a medium effect size for each, depressive and anxiety symptoms separately, and for their combined effects (overall outcome)...
January 18, 2017: Journal of Youth and Adolescence
https://www.readbyqxmd.com/read/28101178/endothelium-dependent-vasodilation-effects-of-panax-notoginseng-and-its-main-components-are-mediated-by-nitric-oxide-and-cyclooxygenase-pathways
#12
Yanyan Wang, Yu Ren, Leilei Xing, Xiangdong Dai, Sheng Liu, Bin Yu, Yi Wang
Panax notoginseng, a traditional Chinese herbal medicine, has been used for the treatment of cardiovascular diseases. The main bioactive components of this species are Panax notoginseng saponins (PNS). The present study aimed to investigate the effects of PNS and five of its main components (ginsenosides Rg1, Re, Rb1 and Rd, and notoginsenoside R1) on rat aorta rings pre-contracted with norepinephrine (NE) and to determine the underlying mechanism of action. Isolated aorta rings (with or without intact endothelium) from adult male Wistar rats were stimulated with NE to induce vasoconstriction, and subsequently treated with different concentrations of PNS and its five main components (Rg1, Re, Rb1, R1 and Rd) separately...
December 2016: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/28100712/salt-management-strategy-defines-the-stem-and-leaf-hydraulic-characteristics-of-six-mangrove-tree-species
#13
Guo-Feng Jiang, Uromi Manage Goodale, Yan-Yan Liu, Guang-You Hao, Kun-Fang Cao
Mangroves in hypersaline coastal habitats are under constant high xylem tension and face great risk of hydraulic dysfunction. To investigate the relationships between functional traits and salt management, we measured 20 hydraulic and photosynthetic traits in four salt-adapted (SA) and two non-SA (NSA) mangrove tree species in south China. The SA species included two salt secretors (SSs), Avicennia marina (Forsskål) Vierhapper and Aegiceras corniculatum (L.) Blanco and two salt excluders (SEs), Bruguiera gymnorrhiza (L...
January 18, 2017: Tree Physiology
https://www.readbyqxmd.com/read/28100636/centriole-splitting-caused-by-loss-of-the-centrosomal-linker-protein-c-nap1-reduces-centriolar-satellite-density-and-impedes-centrosome-amplification
#14
Anne-Marie Flanagan, Elena Stavenschi, Shivakumar Basavaraju, David Gaboriau, David A Hoey, Ciaran G Morrison
Duplication of the centrosomes is a tightly regulated process. Abnormal centrosome numbers can impair cell division and cause changes in how cells migrate. Duplicated centrosomes are held together by a proteinaceous linker made up of rootletin filaments anchored to the centrioles by C-NAP1. This linker is removed in a NEK2A kinase-dependent manner as mitosis begins. To explore C-NAP1 activities in regulating centrosome activities, we used genome editing to ablate it. C-NAP1 null cells were viable and had an increased frequency of premature centriole separation, accompanied by reduced density of the centriolar satellites, with re-expression of C-NAP1 rescuing both these phenotypes...
January 18, 2017: Molecular Biology of the Cell
https://www.readbyqxmd.com/read/28098823/integrative-mirna-gene-expression-analysis-enables-refinement-of-associated-biology-and-prediction-of-response-to-cetuximab-in-head-and-neck-squamous-cell-cancer
#15
Loris De Cecco, Marco Giannoccaro, Edoardo Marchesi, Paolo Bossi, Federica Favales, Laura D Locati, Lisa Licitra, Silvana Pilotti, Silvana Canevari
This paper documents the process by which we, through gene and miRNA expression profiling of the same samples of head and neck squamous cell carcinomas (HNSCC) and an integrative miRNA-mRNA expression analysis, were able to identify candidate biomarkers of progression-free survival (PFS) in patients treated with cetuximab-based approaches. Through sparse partial least square-discriminant analysis (sPLS-DA) and supervised analysis, 36 miRNAs were identified in two components that clearly separated long- and short-PFS patients...
January 14, 2017: Genes
https://www.readbyqxmd.com/read/28098756/detection-of-the-vascular-endothelial-growth-factor-with-a-novel-bioluminescence-resonance-energy-transfer-pair-using-a-two-component-system
#16
Tobias Wimmer, Eva Schroeter, Birgit Lorenz, Knut Stieger
In this paper we describe a two-component BRET (bioluminescence resonance energy transfer)-based method to detect vascular endothelial growth factor (VEGF) molecules in unknown samples as the basis for subsequent in vivo use. A luminescent VEGF binding molecule, which binds in the receptor binding motif of VEGF, is used as the energy donor, transferred to a fluorophore-coupled VEGF binding molecule (acceptor), which binds to the neuropilin binding motif of VEGF, thus enabling energy transfer from the donor to the acceptor molecule...
January 13, 2017: Sensors
https://www.readbyqxmd.com/read/28098500/exploring-black-college-females-perceptions-regarding-hiv-prevention-message-content
#17
Rasheeta Chandler-Coley, Henry Ross, Oluwatobi Ozoya, Celia Lescano, Timothy Flannigan
Media messages can facilitate the delivery of accurate information related to HIV and sexually transmitted infection. This study's purpose was to examine preexisting media campaigns from the iMPPACS study to assess age-, gender-, and culturally appropriate components identified by African American females who attend historically Black colleges/universities. In 3 separate focus group sessions, 31 Black female college students (M age = 20) viewed 4 vignettes and heard 3 audio-only clips, then ranked and commented on them based on perceived satisfaction with HIV prevention content and appropriateness of delivery...
January 18, 2017: Journal of Health Communication
https://www.readbyqxmd.com/read/28098023/key-parameters-for-behaviour-related-to-source-separation-of-household-organic-waste-a-case-study-in-hanoi-vietnam
#18
Kosuke Kawai, Luong Thi Mai Huong
Proper management of food waste, a major component of municipal solid waste (MSW), is needed, especially in developing Asian countries where most MSW is disposed of in landfill sites without any pretreatment. Source separation can contribute to solving problems derived from the disposal of food waste. An organic waste source separation and collection programme has been operated in model areas in Hanoi, Vietnam, since 2007. This study proposed three key parameters (participation rate, proper separation rate and proper discharge rate) for behaviour related to source separation of household organic waste, and monitored the progress of the programme based on the physical composition of household waste sampled from 558 households in model programme areas of Hanoi...
January 1, 2017: Waste Management & Research
https://www.readbyqxmd.com/read/28097282/casimir-effect-between-pinned-particles-in-two-dimensional-jammed-systems
#19
Juan-José Liétor-Santos, Justin C Burton
The Casimir effect arises when long-ranged fluctuations are geometrically confined between two surfaces, leading to a macroscopic force. Traditionally, these forces have been observed in quantum systems and near critical points in classical systems. Here we show the existence of Casimir-like forces between two pinned particles immersed in two-dimensional systems near the jamming transition. We observe two components to the total force: a short-ranged, depletion force and a long-ranged, repulsive Casimir-like force...
January 18, 2017: Soft Matter
https://www.readbyqxmd.com/read/28097277/combining-the-spin-separated-exact-two-component-relativistic-hamiltonian-with-the-equation-of-motion-coupled-cluster-method-for-the-treatment-of-spin-orbit-splittings-of-light-and-heavy-elements
#20
Zhanli Cao, Zhendong Li, Fan Wang, Wenjian Liu
The spin-separated exact two-component (X2C) relativistic Hamiltonian [sf-X2C+so-DKHn, J. Chem. Phys., 2012, 137, 154114] is combined with the equation-of-motion coupled-cluster method with singles and doubles (EOM-CCSD) for the treatment of spin-orbit splittings of open-shell molecular systems. Scalar relativistic effects are treated to infinite order from the outset via the spin-free part of the X2C Hamiltonian (sf-X2C), whereas the spin-orbit couplings (SOC) are handled at the CC level via the first-order Douglas-Kroll-Hess (DKH) type of spin-orbit operator (so-DKH1)...
January 18, 2017: Physical Chemistry Chemical Physics: PCCP
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